AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40559

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40559.t1 MTR_4g102720 51.462 171 50 1 1 171 1 138 6.11e-53 181
MS.gene40559.t1 MTR_5g039760 41.584 202 92 3 2 201 5 182 9.42e-47 163
MS.gene40559.t1 MTR_7g074790 43.678 174 79 2 2 174 5 160 1.23e-39 145
MS.gene40559.t1 MTR_7g061980 50.442 113 55 1 4 115 7 119 9.27e-33 122
MS.gene40559.t1 MTR_0402s0040 38.674 181 105 4 7 183 26 204 2.37e-32 131
MS.gene40559.t1 MTR_1g083640 30.303 231 135 6 3 211 50 276 9.23e-23 99.4
MS.gene40559.t1 MTR_0558s0020 29.741 232 135 7 3 211 35 261 9.43e-23 99.4
MS.gene40559.t1 MTR_6g090505 27.508 309 195 9 36 322 5 306 6.80e-22 98.2
MS.gene40559.t1 MTR_5g015480 43.548 124 67 2 7 127 56 179 5.39e-20 90.5
MS.gene40559.t1 MTR_6g051425 29.224 219 138 7 6 211 13 227 1.73e-19 90.5
MS.gene40559.t1 MTR_2g099430 43.678 87 48 1 30 115 26 112 1.72e-16 76.3
MS.gene40559.t1 MTR_2g070590 38.095 105 62 2 7 108 17 121 2.00e-16 76.3
MS.gene40559.t1 MTR_0337s0040 32.624 141 91 2 75 212 84 223 4.28e-16 78.2
MS.gene40559.t1 MTR_8g446020 31.132 212 119 7 3 211 34 221 7.01e-16 78.6
MS.gene40559.t1 MTR_2g014100 29.469 207 120 6 3 187 43 245 7.36e-16 78.2
MS.gene40559.t1 MTR_1g008290 32.374 139 91 2 64 202 9 144 1.02e-15 75.1
MS.gene40559.t1 MTR_6g017200 43.038 79 44 1 7 84 58 136 2.71e-15 73.9
MS.gene40559.t1 MTR_1g013060 28.037 214 144 4 2 207 19 230 2.81e-14 73.2
MS.gene40559.t1 MTR_1g057920 43.038 79 44 1 7 84 34 112 3.72e-14 69.7
MS.gene40559.t1 MTR_7g113010 36.508 126 65 2 7 130 457 569 1.70e-12 70.9
MS.gene40559.t1 MTR_4g069880 45.312 64 35 0 2 65 5 68 1.91e-12 63.2
MS.gene40559.t1 MTR_1g070355 24.312 218 157 4 13 226 80 293 2.21e-12 70.5
MS.gene40559.t1 MTR_6g022070 26.364 110 81 0 216 325 34 143 5.68e-12 67.8
MS.gene40559.t1 MTR_7g446520 27.513 189 123 2 13 201 94 268 6.18e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40559.t1 AT4G38180 35.784 204 125 4 4 202 70 272 3.16e-29 124
MS.gene40559.t1 AT4G38180 27.632 228 126 3 216 404 408 635 2.19e-22 102
MS.gene40559.t1 AT4G15090 25.753 365 195 8 216 534 384 718 4.23e-28 120
MS.gene40559.t1 AT4G15090 27.962 211 148 4 4 211 48 257 4.90e-20 95.1
MS.gene40559.t1 AT4G15090 25.753 365 195 8 216 534 384 718 4.23e-28 120
MS.gene40559.t1 AT4G15090 27.962 211 148 4 4 211 48 257 4.90e-20 95.1
MS.gene40559.t1 AT4G15090 25.753 365 195 8 216 534 384 718 4.23e-28 120
MS.gene40559.t1 AT4G15090 27.962 211 148 4 4 211 48 257 4.90e-20 95.1
MS.gene40559.t1 AT4G15090 25.753 365 195 8 216 534 384 718 4.23e-28 120
MS.gene40559.t1 AT4G15090 27.962 211 148 4 4 211 48 257 4.90e-20 95.1
MS.gene40559.t1 AT4G15090 25.753 365 195 8 216 534 384 718 4.23e-28 120
MS.gene40559.t1 AT4G15090 27.962 211 148 4 4 211 48 257 4.90e-20 95.1
MS.gene40559.t1 AT3G22170 25.704 284 165 4 208 451 385 662 2.10e-24 108
MS.gene40559.t1 AT3G22170 25.704 284 165 4 208 451 385 662 2.10e-24 108
MS.gene40559.t1 AT4G38170 23.547 327 205 5 216 501 173 495 6.34e-24 106
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 339 616 3.20e-19 92.8
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 339 616 3.20e-19 92.8
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 282 559 4.11e-19 92.0
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 339 616 4.15e-19 92.0
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 339 616 4.15e-19 92.0
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 353 630 5.12e-19 92.0
MS.gene40559.t1 AT4G19990 25.260 289 161 6 223 467 339 616 5.44e-19 92.0
MS.gene40559.t1 AT2G27110 24.723 271 160 4 216 447 355 620 5.78e-18 88.6
MS.gene40559.t1 AT2G27110 24.723 271 160 4 216 447 355 620 5.78e-18 88.6
MS.gene40559.t1 AT2G27110 24.723 271 160 4 216 447 210 475 5.98e-18 88.6
MS.gene40559.t1 AT5G18960 25.087 287 162 7 214 457 503 779 2.96e-17 86.3
MS.gene40559.t1 AT5G18960 27.670 206 106 6 6 207 210 376 1.31e-12 71.6
MS.gene40559.t1 AT5G18960 25.087 287 162 7 214 457 503 779 2.96e-17 86.3
MS.gene40559.t1 AT5G18960 27.670 206 106 6 6 207 210 376 1.31e-12 71.6
MS.gene40559.t1 AT1G52520 22.337 291 173 5 219 458 407 695 6.10e-16 82.0
MS.gene40559.t1 AT1G76320 28.947 228 142 8 11 235 1 211 1.61e-15 80.9
MS.gene40559.t1 AT1G76320 28.947 228 142 8 11 235 1 211 1.61e-15 80.5
MS.gene40559.t1 AT1G76320 28.947 228 142 8 11 235 1 211 1.74e-15 80.5
MS.gene40559.t1 AT1G76320 28.947 228 142 8 11 235 1 211 1.74e-15 80.5
MS.gene40559.t1 AT3G06250 28.713 202 101 6 6 204 189 350 7.87e-12 68.9
MS.gene40559.t1 AT3G06250 28.713 202 101 6 6 204 189 350 7.87e-12 68.9
MS.gene40559.t1 AT3G06250 28.713 202 101 6 6 204 189 350 7.87e-12 68.9
MS.gene40559.t1 AT5G28530 28.070 171 115 5 5 168 53 222 8.91e-11 65.5
MS.gene40559.t1 AT5G28530 28.070 171 115 5 5 168 53 222 8.91e-11 65.5
MS.gene40559.t1 AT5G28530 28.070 171 115 5 5 168 53 222 9.18e-11 65.5
MS.gene40559.t1 AT5G28530 28.070 171 115 5 5 168 53 222 9.44e-11 65.5

Find 103 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCACCTCTTTAAAATATA+AGG 0.176322 2.3:-19664798 None:intergenic
CAGGCCTCCATTCAAGTTTC+TGG 0.199038 2.3:-19665728 None:intergenic
TTGAAGATGCTTGGGAGTTC+TGG 0.234863 2.3:+19663304 MS.gene40559:CDS
TTAAGAAACTCGACAGCTTC+TGG 0.244592 2.3:-19664957 None:intergenic
GACATCATATGAGTTGTTTC+TGG 0.246470 2.3:-19663582 None:intergenic
AAATTTGTCCCCGCTTTATT+AGG 0.254015 2.3:+19664910 MS.gene40559:CDS
CCGAAAACCATATCTACTTT+TGG 0.265147 2.3:-19663276 None:intergenic
TATCTCTTTAATATTGTTAG+AGG 0.283905 2.3:-19665057 None:intergenic
TATTGCCAAGAAAAGGAATA+TGG 0.289005 2.3:+19664641 MS.gene40559:CDS
ATCTCGAGCACAATGTTATA+AGG 0.298579 2.3:+19664887 MS.gene40559:CDS
CCTTGCTTCACAATTTGTTC+TGG 0.300300 2.3:-19663484 None:intergenic
CATATAGGTGCACTTGTAAT+TGG 0.302297 2.3:-19665449 None:intergenic
CTTAAAAGTCTTCATTCATA+TGG 0.313141 2.3:+19664755 MS.gene40559:CDS
CTTTGCACGTTTCTTTCTCT+TGG 0.317009 2.3:-19665180 None:intergenic
ATGAAGAAGACTTTGACATT+AGG 0.337357 2.3:+19664423 MS.gene40559:CDS
ACGGTGGTAAGGTTGGATTC+GGG 0.338362 2.3:+19663334 MS.gene40559:CDS
CAATTACAAGTGCACCTATA+TGG 0.342061 2.3:+19665450 MS.gene40559:CDS
TGTACAATATAAAGGAGAAA+TGG 0.347671 2.3:+19664388 MS.gene40559:CDS
TAAGGAAGTTGTTGATGAAT+TGG 0.347944 2.3:+19664977 MS.gene40559:CDS
GCATACAATCAAACCATCTA+TGG 0.348748 2.3:+19665482 MS.gene40559:CDS
TGTCGTAAATTTGAGAGCTT+TGG 0.348811 2.3:+19664714 MS.gene40559:CDS
GATGGAACATCTAGTCAATT+TGG 0.363982 2.3:+19665586 MS.gene40559:CDS
TGTTATTAGCTAACCATAGA+TGG 0.365422 2.3:-19665495 None:intergenic
TGAAGAAGACTTTGACATTA+GGG 0.366463 2.3:+19664424 MS.gene40559:CDS
TACGGTGGTAAGGTTGGATT+CGG 0.368578 2.3:+19663333 MS.gene40559:CDS
ATGGCAAGTGACCTTCGATT+TGG 0.384310 2.3:+19664668 MS.gene40559:CDS
TTTGTTCTGGTCTCTAGTCT+TGG 0.387348 2.3:-19663471 None:intergenic
ATGGTTCAGTTTCATCTTGT+AGG 0.389892 2.3:+19663388 MS.gene40559:CDS
TTGGAAGCCAAAAGTAGATA+TGG 0.391013 2.3:+19663269 MS.gene40559:CDS
GCAAAGAATGTACAATATAA+AGG 0.392434 2.3:+19664380 MS.gene40559:CDS
TCGGTAGCTTTGAAGATGCT+TGG 0.398691 2.3:+19663295 MS.gene40559:CDS
CGGTAGCTTTGAAGATGCTT+GGG 0.400248 2.3:+19663296 MS.gene40559:CDS
AGAAGCTGTCGAGTTTCTTA+AGG 0.400377 2.3:+19664959 MS.gene40559:CDS
GATACAAGATGAATGAAGAT+TGG 0.401677 2.3:+19663250 None:intergenic
GGCTTTAAGAAATGCATGTA+TGG 0.412867 2.3:+19664279 MS.gene40559:CDS
TATGCATTATTAAGCCATAT+AGG 0.412953 2.3:-19665464 None:intergenic
TATGATGTCTTCTCAACAAA+AGG 0.432515 2.3:+19663596 MS.gene40559:CDS
GTACAATATAAAGGAGAAAT+GGG 0.433816 2.3:+19664389 MS.gene40559:CDS
GATGGTGCAACACAAGTAAA+GGG 0.435850 2.3:+19665092 MS.gene40559:CDS
GTAAGGTTGGATTCGGGGTG+AGG 0.436672 2.3:+19663340 MS.gene40559:CDS
GAGAAAGAAGTTTGGTACAA+GGG 0.449206 2.3:+19663769 MS.gene40559:CDS
AAGAACAATTTGAAGATGGT+TGG 0.449713 2.3:+19664313 MS.gene40559:CDS
GTTCAAAACGTTGGAAAGGT+TGG 0.454209 2.3:+19665141 MS.gene40559:CDS
CTTATGTTATTGCCAAGAAA+AGG 0.457771 2.3:+19664634 MS.gene40559:CDS
GATTTACTGAGTAAAGAAGT+AGG 0.459354 2.3:+19663687 MS.gene40559:CDS
AGGTTGGGTACAAACGAAAC+TGG 0.462843 2.3:+19665157 MS.gene40559:CDS
AGTAGAGCTTCACAAACTAT+TGG 0.464878 2.3:+19664834 MS.gene40559:CDS
AGGTTTGTTTGCTGCAAAGA+AGG 0.468354 2.3:+19663408 MS.gene40559:CDS
TGATGGTGCAACACAAGTAA+AGG 0.471729 2.3:+19665091 MS.gene40559:CDS
TTGATCAAAAGAATTATCTT+CGG 0.474061 2.3:+19663739 MS.gene40559:CDS
ACAAATTGTGAAGCAAGGTT+AGG 0.474466 2.3:+19663489 MS.gene40559:CDS
TTCACAAATTCAAGAACCTA+TGG 0.484743 2.3:+19665211 MS.gene40559:CDS
ACATTGTAAATCACTAATGT+TGG 0.488437 2.3:-19665408 None:intergenic
TGGGAGTTCTGGAAAACTTA+CGG 0.491418 2.3:+19663315 MS.gene40559:CDS
CTTGTAATTGGCGATGCATA+TGG 0.492051 2.3:-19665437 None:intergenic
GAATGTGGATGGAAAATTGA+TGG 0.494783 2.3:+19663518 MS.gene40559:CDS
CTGGAAAACTTACGGTGGTA+AGG 0.495710 2.3:+19663323 MS.gene40559:CDS
TTCAAAACGTTGGAAAGGTT+GGG 0.496100 2.3:+19665142 MS.gene40559:CDS
CTCTTTAAAATATAAGGCTC+AGG 0.500537 2.3:-19664792 None:intergenic
GAGATATCAAGTACTAATGA+TGG 0.502322 2.3:+19665074 MS.gene40559:CDS
GATGAAGAACAATTTGAAGA+TGG 0.510431 2.3:+19664309 MS.gene40559:CDS
TTCTTGAATTTGTGAAGCAG+TGG 0.516062 2.3:-19665204 None:intergenic
ATTGCCAAGAAAAGGAATAT+GGG 0.518068 2.3:+19664642 MS.gene40559:CDS
AGAAAAGGAATATGGGAGAA+TGG 0.522592 2.3:+19664649 MS.gene40559:CDS
AGAAAGAAGTTTGGTACAAG+GGG 0.523858 2.3:+19663770 MS.gene40559:CDS
TAGTCATGTGGTAGAAGACT+TGG 0.525523 2.3:+19664860 MS.gene40559:CDS
AGAGAAAGAAGTTTGGTACA+AGG 0.525956 2.3:+19663768 MS.gene40559:CDS
ATAAGTAGTGAACAAGTGAA+TGG 0.530525 2.3:+19665032 MS.gene40559:CDS
AGTTTGGTACAAGGGGAGGC+TGG 0.532184 2.3:+19663777 MS.gene40559:CDS
AAAACTTACGGTGGTAAGGT+TGG 0.535426 2.3:+19663327 MS.gene40559:CDS
CGGTGGTAAGGTTGGATTCG+GGG 0.537232 2.3:+19663335 MS.gene40559:CDS
TGATAAGCATGGTAGAAAGT+TGG 0.539495 2.3:-19663840 None:intergenic
CGATTTCCTTTGTTGTACAC+CGG 0.540939 2.3:-19663661 None:intergenic
TGTGACGATCCTAATAAAGC+GGG 0.542482 2.3:-19664919 None:intergenic
TTAGGACTTAAGAATGTGGA+TGG 0.548535 2.3:+19663507 MS.gene40559:CDS
GATGGTACCAGAAACTTGAA+TGG 0.549108 2.3:+19665721 MS.gene40559:CDS
AAGATGTTGTAGAAGAAAAG+CGG 0.551004 2.3:+19664541 MS.gene40559:CDS
AAGGGGTTTAAGAACAAAGA+AGG 0.557368 2.3:+19665110 MS.gene40559:CDS
GCAAACGTAACTCGTGGTTG+TGG 0.557451 2.3:-19663560 None:intergenic
AAGAACAAAGAAGGTCCAAA+GGG 0.561141 2.3:+19665119 MS.gene40559:CDS
TTATAGGAGCAACTTGATGA+TGG 0.570385 2.3:+19665703 MS.gene40559:intron
TAAGAACAAAGAAGGTCCAA+AGG 0.577271 2.3:+19665118 MS.gene40559:CDS
AAGGGTTCAAAACGTTGGAA+AGG 0.583511 2.3:+19665137 MS.gene40559:CDS
GTCCAAAGGGTTCAAAACGT+TGG 0.588871 2.3:+19665132 MS.gene40559:CDS
TAGAAGAACAAAGGTAAGCA+AGG 0.590187 2.3:+19665311 MS.gene40559:intron
TTTATTAGGATCGTCACAGA+AGG 0.594701 2.3:+19664924 MS.gene40559:CDS
AAGAAGTTTGGTACAAGGGG+AGG 0.610658 2.3:+19663773 MS.gene40559:CDS
CCAGAACAAATTGTGAAGCA+AGG 0.610911 2.3:+19663484 MS.gene40559:CDS
AACTATTGGTGTTAGTCATG+TGG 0.613627 2.3:+19664848 MS.gene40559:CDS
GTAGGAGGCAGAACTAACTC+TGG 0.615242 2.3:+19663705 MS.gene40559:CDS
TTTCTGGCAAACGTAACTCG+TGG 0.616832 2.3:-19663566 None:intergenic
CTGTGACGATCCTAATAAAG+CGG 0.623114 2.3:-19664920 None:intergenic
CGGGATGAAGTGTCAAACAC+AGG 0.627178 2.3:+19665353 MS.gene40559:CDS
ATTGAACTAGCTGATAGTGC+CGG 0.644559 2.3:+19663642 MS.gene40559:CDS
CAATATCTAGCTGATAAGCA+TGG 0.647406 2.3:-19663851 None:intergenic
ATAGTGCCGGTGTACAACAA+AGG 0.660446 2.3:+19663655 MS.gene40559:CDS
GGTACCAGAAACTTGAATGG+AGG 0.672855 2.3:+19665724 MS.gene40559:CDS
AGCATGCTTTGTGAAAAGCA+TGG 0.679180 2.3:+19664590 MS.gene40559:CDS
ATGGTGCAACACAAGTAAAG+GGG 0.679306 2.3:+19665093 MS.gene40559:CDS
AAGGTTAGGACTTAAGAATG+TGG 0.679784 2.3:+19663503 MS.gene40559:CDS
GTGACGATCCTAATAAAGCG+GGG 0.705450 2.3:-19664918 None:intergenic
TTACTGAGTAAAGAAGTAGG+AGG 0.709735 2.3:+19663690 MS.gene40559:CDS
GAGTTCTGGAAAACTTACGG+TGG 0.762461 2.3:+19663318 MS.gene40559:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATTAATAATACCAT+AGG - chr2.3:19665230-19665249 None:intergenic 10.0%
!!! TATTAAAAATTTTATGAGTA+AGG + chr2.3:19664485-19664504 MS.gene40559:CDS 10.0%
!! ACAAAAAAAAAGGAATATAA+AGG - chr2.3:19665290-19665309 None:intergenic 15.0%
!!! AATTATTTTGGTTTTTATGT+AGG + chr2.3:19665558-19665577 MS.gene40559:intron 15.0%
!!! ACTTGCATTTTTTAAATTAT+AGG + chr2.3:19665687-19665706 MS.gene40559:intron 15.0%
!!! ATTATTTTGGTTTTTATGTA+GGG + chr2.3:19665559-19665578 MS.gene40559:intron 15.0%
!!! GTTTAGTGTTTAAATTATTT+TGG + chr2.3:19665546-19665565 MS.gene40559:intron 15.0%
!!! TTGATTGATTACAATTATTT+TGG + chr2.3:19663915-19663934 MS.gene40559:intron 15.0%
!! TATCTCTTTAATATTGTTAG+AGG - chr2.3:19665060-19665079 None:intergenic 20.0%
!! TTAAACACTAAACATAGTTA+AGG - chr2.3:19665539-19665558 None:intergenic 20.0%
!! TTGATCAAAAGAATTATCTT+CGG + chr2.3:19663739-19663758 MS.gene40559:CDS 20.0%
! AAGGTTCCAAAAATATAAAG+AGG + chr2.3:19665330-19665349 MS.gene40559:CDS 25.0%
! ACACTTAGGAAATTTAATGA+AGG + chr2.3:19664236-19664255 MS.gene40559:intron 25.0%
! ACATTGTAAATCACTAATGT+TGG - chr2.3:19665411-19665430 None:intergenic 25.0%
! ACTTAGGAAATTTAATGAAG+GGG + chr2.3:19664238-19664257 MS.gene40559:intron 25.0%
! AGATTATACACACAGAAAAA+AGG + chr2.3:19663365-19663384 MS.gene40559:CDS 25.0%
! CAAAATGGAATTAAACACTT+AGG + chr2.3:19664222-19664241 MS.gene40559:intron 25.0%
! CACTTAGGAAATTTAATGAA+GGG + chr2.3:19664237-19664256 MS.gene40559:intron 25.0%
! CTTAAAAGTCTTCATTCATA+TGG + chr2.3:19664755-19664774 MS.gene40559:CDS 25.0%
! GAAAAAAAGAGAAAGAAGTT+TGG + chr2.3:19663761-19663780 MS.gene40559:CDS 25.0%
! GCAAAGAATGTACAATATAA+AGG + chr2.3:19664380-19664399 MS.gene40559:CDS 25.0%
! GTACAATATAAAGGAGAAAT+GGG + chr2.3:19664389-19664408 MS.gene40559:CDS 25.0%
! GTTCTTCTACACAAAAAAAA+AGG - chr2.3:19665300-19665319 None:intergenic 25.0%
! TATGCATTATTAAGCCATAT+AGG - chr2.3:19665467-19665486 None:intergenic 25.0%
! TGTACAATATAAAGGAGAAA+TGG + chr2.3:19664388-19664407 MS.gene40559:CDS 25.0%
!! AAATTTTATGAGTAAGGACT+TGG + chr2.3:19664491-19664510 MS.gene40559:CDS 25.0%
!!! TAGTTTTACTAGCTTATTGA+TGG + chr2.3:19665615-19665634 MS.gene40559:CDS 25.0%
!!! TTTTTTGTGTAGAAGAACAA+AGG + chr2.3:19665302-19665321 MS.gene40559:intron 25.0%
AAGAACAATTTGAAGATGGT+TGG + chr2.3:19664313-19664332 MS.gene40559:CDS 30.0%
AAGATGTTGTAGAAGAAAAG+CGG + chr2.3:19664541-19664560 MS.gene40559:CDS 30.0%
ATAAGTAGTGAACAAGTGAA+TGG + chr2.3:19665032-19665051 MS.gene40559:CDS 30.0%
ATGAAGAAGACTTTGACATT+AGG + chr2.3:19664423-19664442 MS.gene40559:CDS 30.0%
ATGGTGTTCAGAAAAATGAT+TGG + chr2.3:19664355-19664374 MS.gene40559:CDS 30.0%
ATTGCCAAGAAAAGGAATAT+GGG + chr2.3:19664642-19664661 MS.gene40559:CDS 30.0%
CTTAGGAAATTTAATGAAGG+GGG + chr2.3:19664239-19664258 MS.gene40559:intron 30.0%
CTTATGTTATTGCCAAGAAA+AGG + chr2.3:19664634-19664653 MS.gene40559:CDS 30.0%
GAGATATCAAGTACTAATGA+TGG + chr2.3:19665074-19665093 MS.gene40559:CDS 30.0%
GATGAAGAACAATTTGAAGA+TGG + chr2.3:19664309-19664328 MS.gene40559:CDS 30.0%
GATTTACTGAGTAAAGAAGT+AGG + chr2.3:19663687-19663706 MS.gene40559:CDS 30.0%
GTTCCAAAAATATAAAGAGG+CGG + chr2.3:19665333-19665352 MS.gene40559:CDS 30.0%
TACTGATCAAGACAAATCAA+TGG + chr2.3:19664136-19664155 MS.gene40559:intron 30.0%
TATACACACAGAAAAAAGGA+TGG + chr2.3:19663369-19663388 MS.gene40559:CDS 30.0%
TATGATGTCTTCTCAACAAA+AGG + chr2.3:19663596-19663615 MS.gene40559:CDS 30.0%
TATTGCCAAGAAAAGGAATA+TGG + chr2.3:19664641-19664660 MS.gene40559:CDS 30.0%
TGAAGAAGACTTTGACATTA+GGG + chr2.3:19664424-19664443 MS.gene40559:CDS 30.0%
TGATCAGTAAAAACTGTTTG+AGG - chr2.3:19664125-19664144 None:intergenic 30.0%
TGTTATTAGCTAACCATAGA+TGG - chr2.3:19665498-19665517 None:intergenic 30.0%
TTCACAAATTCAAGAACCTA+TGG + chr2.3:19665211-19665230 MS.gene40559:CDS 30.0%
TTCCAAAAATATAAAGAGGC+GGG + chr2.3:19665334-19665353 MS.gene40559:CDS 30.0%
! CCAAAAGTAGATATGGTTTT+CGG + chr2.3:19663276-19663295 MS.gene40559:CDS 30.0%
! TAAGGAAGTTGTTGATGAAT+TGG + chr2.3:19664977-19664996 MS.gene40559:CDS 30.0%
! TGTATTCTTTTCCAAATCGA+AGG - chr2.3:19664682-19664701 None:intergenic 30.0%
!! AACACCATACTTTTTAAGGA+GGG - chr2.3:19664343-19664362 None:intergenic 30.0%
!! AGCCTTATATTTTAAAGAGG+TGG + chr2.3:19664796-19664815 MS.gene40559:CDS 30.0%
!! CTCTTTAAAATATAAGGCTC+AGG - chr2.3:19664795-19664814 None:intergenic 30.0%
!! CTGAGCCTTATATTTTAAAG+AGG + chr2.3:19664793-19664812 MS.gene40559:CDS 30.0%
!! GTCCACCTCTTTAAAATATA+AGG - chr2.3:19664801-19664820 None:intergenic 30.0%
!! TCTGAACACCATACTTTTTA+AGG - chr2.3:19664347-19664366 None:intergenic 30.0%
AAGAACAAAGAAGGTCCAAA+GGG + chr2.3:19665119-19665138 MS.gene40559:CDS 35.0%
AAGGGGTTTAAGAACAAAGA+AGG + chr2.3:19665110-19665129 MS.gene40559:CDS 35.0%
AAGGTTAGGACTTAAGAATG+TGG + chr2.3:19663503-19663522 MS.gene40559:CDS 35.0%
ACAAATTGTGAAGCAAGGTT+AGG + chr2.3:19663489-19663508 MS.gene40559:CDS 35.0%
ACATCCCTCCTTAAAAAGTA+TGG + chr2.3:19664336-19664355 MS.gene40559:CDS 35.0%
AGAAAAGGAATATGGGAGAA+TGG + chr2.3:19664649-19664668 MS.gene40559:CDS 35.0%
AGAAAGAAGTTTGGTACAAG+GGG + chr2.3:19663770-19663789 MS.gene40559:CDS 35.0%
AGAGAAAGAAGTTTGGTACA+AGG + chr2.3:19663768-19663787 MS.gene40559:CDS 35.0%
AGTAGAGCTTCACAAACTAT+TGG + chr2.3:19664834-19664853 MS.gene40559:CDS 35.0%
ATCTCGAGCACAATGTTATA+AGG + chr2.3:19664887-19664906 MS.gene40559:CDS 35.0%
ATGGTTCAGTTTCATCTTGT+AGG + chr2.3:19663388-19663407 MS.gene40559:CDS 35.0%
CAATATCTAGCTGATAAGCA+TGG - chr2.3:19663854-19663873 None:intergenic 35.0%
CAATTACAAGTGCACCTATA+TGG + chr2.3:19665450-19665469 MS.gene40559:CDS 35.0%
CATATAGGTGCACTTGTAAT+TGG - chr2.3:19665452-19665471 None:intergenic 35.0%
GAATGTGGATGGAAAATTGA+TGG + chr2.3:19663518-19663537 MS.gene40559:CDS 35.0%
GAGAAAGAAGTTTGGTACAA+GGG + chr2.3:19663769-19663788 MS.gene40559:CDS 35.0%
GATGGAACATCTAGTCAATT+TGG + chr2.3:19665586-19665605 MS.gene40559:CDS 35.0%
GCATACAATCAAACCATCTA+TGG + chr2.3:19665482-19665501 MS.gene40559:CDS 35.0%
TAAGAACAAAGAAGGTCCAA+AGG + chr2.3:19665118-19665137 MS.gene40559:CDS 35.0%
TAGAAGAACAAAGGTAAGCA+AGG + chr2.3:19665311-19665330 MS.gene40559:intron 35.0%
TGATAAGCATGGTAGAAAGT+TGG - chr2.3:19663843-19663862 None:intergenic 35.0%
TGTCGTAAATTTGAGAGCTT+TGG + chr2.3:19664714-19664733 MS.gene40559:CDS 35.0%
TTACTGAGTAAAGAAGTAGG+AGG + chr2.3:19663690-19663709 MS.gene40559:CDS 35.0%
TTAGGACTTAAGAATGTGGA+TGG + chr2.3:19663507-19663526 MS.gene40559:CDS 35.0%
TTATAGGAGCAACTTGATGA+TGG + chr2.3:19665703-19665722 MS.gene40559:intron 35.0%
TTCAAAACGTTGGAAAGGTT+GGG + chr2.3:19665142-19665161 MS.gene40559:CDS 35.0%
TTCTTGAATTTGTGAAGCAG+TGG - chr2.3:19665207-19665226 None:intergenic 35.0%
TTGGAAGCCAAAAGTAGATA+TGG + chr2.3:19663269-19663288 MS.gene40559:CDS 35.0%
TTTATTAGGATCGTCACAGA+AGG + chr2.3:19664924-19664943 MS.gene40559:CDS 35.0%
! AAATTTGTCCCCGCTTTATT+AGG + chr2.3:19664910-19664929 MS.gene40559:CDS 35.0%
! ATCAGATCACCAATGTGTTT+TGG + chr2.3:19663877-19663896 MS.gene40559:intron 35.0%
! ATCCCGCCTCTTTATATTTT+TGG - chr2.3:19665339-19665358 None:intergenic 35.0%
! CCGAAAACCATATCTACTTT+TGG - chr2.3:19663279-19663298 None:intergenic 35.0%
! GACATCATATGAGTTGTTTC+TGG - chr2.3:19663585-19663604 None:intergenic 35.0%
! GTTTTATGATCTTGCTTGTC+TGG - chr2.3:19663444-19663463 None:intergenic 35.0%
! TCAGATCACCAATGTGTTTT+GGG + chr2.3:19663878-19663897 MS.gene40559:intron 35.0%
! TTCTCCCATATTCCTTTTCT+TGG - chr2.3:19664649-19664668 None:intergenic 35.0%
!! AACTATTGGTGTTAGTCATG+TGG + chr2.3:19664848-19664867 MS.gene40559:CDS 35.0%
!! GAACACCATACTTTTTAAGG+AGG - chr2.3:19664344-19664363 None:intergenic 35.0%
!! GGCTTTAAGAAATGCATGTA+TGG + chr2.3:19664279-19664298 MS.gene40559:CDS 35.0%
!!! ATTGATGGTGCATGATTTTG+TGG + chr2.3:19663533-19663552 MS.gene40559:CDS 35.0%
!!! GGGGAATCTTATTTTCTTAC+TGG + chr2.3:19664258-19664277 MS.gene40559:CDS 35.0%
AAAACTTACGGTGGTAAGGT+TGG + chr2.3:19663327-19663346 MS.gene40559:CDS 40.0%
AAGGGTTCAAAACGTTGGAA+AGG + chr2.3:19665137-19665156 MS.gene40559:CDS 40.0%
ACATAAACCATGGTAAGCTC+CGG - chr2.3:19664187-19664206 None:intergenic 40.0%
AGAAGCTGTCGAGTTTCTTA+AGG + chr2.3:19664959-19664978 MS.gene40559:CDS 40.0%
AGCATGCTTTGTGAAAAGCA+TGG + chr2.3:19664590-19664609 MS.gene40559:CDS 40.0%
ATGGTGCAACACAAGTAAAG+GGG + chr2.3:19665093-19665112 MS.gene40559:CDS 40.0%
CAAAAATTACAGCTCCTCGA+TGG - chr2.3:19664019-19664038 None:intergenic 40.0%
CCAGAACAAATTGTGAAGCA+AGG + chr2.3:19663484-19663503 MS.gene40559:CDS 40.0%
CTGTGACGATCCTAATAAAG+CGG - chr2.3:19664923-19664942 None:intergenic 40.0%
CTTACCATGGTTTATGTACG+TGG + chr2.3:19664190-19664209 MS.gene40559:intron 40.0%
CTTGTAATTGGCGATGCATA+TGG - chr2.3:19665440-19665459 None:intergenic 40.0%
CTTTGCACGTTTCTTTCTCT+TGG - chr2.3:19665183-19665202 None:intergenic 40.0%
GATGGTACCAGAAACTTGAA+TGG + chr2.3:19665721-19665740 MS.gene40559:CDS 40.0%
GATGGTGCAACACAAGTAAA+GGG + chr2.3:19665092-19665111 MS.gene40559:CDS 40.0%
GTTCAAAACGTTGGAAAGGT+TGG + chr2.3:19665141-19665160 MS.gene40559:CDS 40.0%
TAGTCATGTGGTAGAAGACT+TGG + chr2.3:19664860-19664879 MS.gene40559:CDS 40.0%
TGTGACGATCCTAATAAAGC+GGG - chr2.3:19664922-19664941 None:intergenic 40.0%
TTAAGAAACTCGACAGCTTC+TGG - chr2.3:19664960-19664979 None:intergenic 40.0%
TTTGTTCTGGTCTCTAGTCT+TGG - chr2.3:19663474-19663493 None:intergenic 40.0%
! AGGTTTGTTTGCTGCAAAGA+AGG + chr2.3:19663408-19663427 MS.gene40559:CDS 40.0%
! ATTGAACTAGCTGATAGTGC+CGG + chr2.3:19663642-19663661 MS.gene40559:CDS 40.0%
! CATCGAGGAGCTGTAATTTT+TGG + chr2.3:19664017-19664036 MS.gene40559:intron 40.0%
! CCTTGCTTCACAATTTGTTC+TGG - chr2.3:19663487-19663506 None:intergenic 40.0%
! CGATTTCCTTTGTTGTACAC+CGG - chr2.3:19663664-19663683 None:intergenic 40.0%
! GTTTTTATGTAGGGAGACGA+TGG + chr2.3:19665568-19665587 MS.gene40559:intron 40.0%
! TGATGGTGCAACACAAGTAA+AGG + chr2.3:19665091-19665110 MS.gene40559:CDS 40.0%
!! TGGGAGTTCTGGAAAACTTA+CGG + chr2.3:19663315-19663334 MS.gene40559:CDS 40.0%
!! TTCCAACGTTTTGAACCCTT+TGG - chr2.3:19665137-19665156 None:intergenic 40.0%
AAACTGCTGAGTCATACGAG+TGG + chr2.3:19664060-19664079 MS.gene40559:intron 45.0%
AAATTACAGCTCCTCGATGG+TGG - chr2.3:19664016-19664035 None:intergenic 45.0%
AAGAAGTTTGGTACAAGGGG+AGG + chr2.3:19663773-19663792 MS.gene40559:CDS 45.0%
AATCCCGAGATGATTGCTAG+TGG - chr2.3:19663990-19664009 None:intergenic 45.0%
ACGTGGCACTTGATGCAAAA+TGG + chr2.3:19664207-19664226 MS.gene40559:intron 45.0%
AGACCACTAGCAATCATCTC+GGG + chr2.3:19663984-19664003 MS.gene40559:intron 45.0%
AGCATCTGCCCAAAACACAT+TGG - chr2.3:19663889-19663908 None:intergenic 45.0%
AGGTTGGGTACAAACGAAAC+TGG + chr2.3:19665157-19665176 MS.gene40559:CDS 45.0%
AGTGCCACGTACATAAACCA+TGG - chr2.3:19664197-19664216 None:intergenic 45.0%
ATGGCAAGTGACCTTCGATT+TGG + chr2.3:19664668-19664687 MS.gene40559:CDS 45.0%
CAGACCACTAGCAATCATCT+CGG + chr2.3:19663983-19664002 MS.gene40559:intron 45.0%
CATAAACCATGGTAAGCTCC+GGG - chr2.3:19664186-19664205 None:intergenic 45.0%
CTGGAAAACTTACGGTGGTA+AGG + chr2.3:19663323-19663342 MS.gene40559:CDS 45.0%
GGTACCAGAAACTTGAATGG+AGG + chr2.3:19665724-19665743 MS.gene40559:CDS 45.0%
GTCCAAAGGGTTCAAAACGT+TGG + chr2.3:19665132-19665151 MS.gene40559:CDS 45.0%
GTGACGATCCTAATAAAGCG+GGG - chr2.3:19664921-19664940 None:intergenic 45.0%
TACGGTGGTAAGGTTGGATT+CGG + chr2.3:19663333-19663352 MS.gene40559:CDS 45.0%
TTGAAGATGCTTGGGAGTTC+TGG + chr2.3:19663304-19663323 MS.gene40559:CDS 45.0%
! TTTCTGGCAAACGTAACTCG+TGG - chr2.3:19663569-19663588 None:intergenic 45.0%
!! AATGGCAAAAGCATTAGCCG+AGG + chr2.3:19664154-19664173 MS.gene40559:intron 45.0%
!! ATAGTGCCGGTGTACAACAA+AGG + chr2.3:19663655-19663674 MS.gene40559:CDS 45.0%
!! ATGTGTTTTGGGCAGATGCT+AGG + chr2.3:19663889-19663908 MS.gene40559:intron 45.0%
!! CGGTAGCTTTGAAGATGCTT+GGG + chr2.3:19663296-19663315 MS.gene40559:CDS 45.0%
!! GAGTTCTGGAAAACTTACGG+TGG + chr2.3:19663318-19663337 MS.gene40559:CDS 45.0%
!! TCGGTAGCTTTGAAGATGCT+TGG + chr2.3:19663295-19663314 MS.gene40559:CDS 45.0%
ACGGTGGTAAGGTTGGATTC+GGG + chr2.3:19663334-19663353 MS.gene40559:CDS 50.0%
CATTAGCCGAGGTTAATGCC+CGG + chr2.3:19664165-19664184 MS.gene40559:intron 50.0%
GCAAACGTAACTCGTGGTTG+TGG - chr2.3:19663563-19663582 None:intergenic 50.0%
GTAGGAGGCAGAACTAACTC+TGG + chr2.3:19663705-19663724 MS.gene40559:CDS 50.0%
TAAGCTCCGGGCATTAACCT+CGG - chr2.3:19664174-19664193 None:intergenic 50.0%
TCGGGATTTAACCACCATCG+AGG + chr2.3:19664002-19664021 MS.gene40559:intron 50.0%
TTAATGCCCGGAGCTTACCA+TGG + chr2.3:19664177-19664196 MS.gene40559:intron 50.0%
! CAGGCCTCCATTCAAGTTTC+TGG - chr2.3:19665731-19665750 None:intergenic 50.0%
! CGGGATGAAGTGTCAAACAC+AGG + chr2.3:19665353-19665372 MS.gene40559:CDS 50.0%
AGTTTGGTACAAGGGGAGGC+TGG + chr2.3:19663777-19663796 MS.gene40559:CDS 55.0%
CGGTGGTAAGGTTGGATTCG+GGG + chr2.3:19663335-19663354 MS.gene40559:CDS 55.0%
GTAAGGTTGGATTCGGGGTG+AGG + chr2.3:19663340-19663359 MS.gene40559:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 19663258 19665753 19663258 ID=MS.gene40559
chr2.3 mRNA 19663258 19665753 19663258 ID=MS.gene40559.t1;Parent=MS.gene40559
chr2.3 exon 19663258 19663890 19663258 ID=MS.gene40559.t1.exon1;Parent=MS.gene40559.t1
chr2.3 CDS 19663258 19663890 19663258 ID=cds.MS.gene40559.t1;Parent=MS.gene40559.t1
chr2.3 exon 19664258 19665232 19664258 ID=MS.gene40559.t1.exon2;Parent=MS.gene40559.t1
chr2.3 CDS 19664258 19665232 19664258 ID=cds.MS.gene40559.t1;Parent=MS.gene40559.t1
chr2.3 exon 19665314 19665503 19665314 ID=MS.gene40559.t1.exon3;Parent=MS.gene40559.t1
chr2.3 CDS 19665314 19665503 19665314 ID=cds.MS.gene40559.t1;Parent=MS.gene40559.t1
chr2.3 exon 19665584 19665636 19665584 ID=MS.gene40559.t1.exon4;Parent=MS.gene40559.t1
chr2.3 CDS 19665584 19665636 19665584 ID=cds.MS.gene40559.t1;Parent=MS.gene40559.t1
chr2.3 exon 19665709 19665753 19665709 ID=MS.gene40559.t1.exon5;Parent=MS.gene40559.t1
chr2.3 CDS 19665709 19665753 19665709 ID=cds.MS.gene40559.t1;Parent=MS.gene40559.t1
Gene Sequence

>MS.gene40559

ATGAATGAAGATTGGAAGCCAAAAGTAGATATGGTTTTCGGTAGCTTTGAAGATGCTTGGGAGTTCTGGAAAACTTACGGTGGTAAGGTTGGATTCGGGGTGAGGAAAGATTATACACACAGAAAAAAGGATGGTTCAGTTTCATCTTGTAGGTTTGTTTGCTGCAAAGAAGGCATACGAAAGCCAGACAAGCAAGATCATAAAACAAAGAATCCAAGACTAGAGACCAGAACAAATTGTGAAGCAAGGTTAGGACTTAAGAATGTGGATGGAAAATTGATGGTGCATGATTTTGTGGAAGACCACAACCACGAGTTACGTTTGCCAGAAACAACTCATATGATGTCTTCTCAACAAAAGGTTTCTGAAATTCAATGTCAACAAATTGAACTAGCTGATAGTGCCGGTGTACAACAAAGGAAATCGTTTGATTTACTGAGTAAAGAAGTAGGAGGCAGAACTAACTCTGGTTTTATTCGTCTTGATCAAAAGAATTATCTTCGGAAAAAAAGAGAAAGAAGTTTGGTACAAGGGGAGGCTGGCTATTTACTGCAATACTTTCAAAGAAAGTCAGTAGAAAATCCAACTTTCTACCATGCTTATCAGCTAGATATTGAAGATCAGATCACCAATGTGTTTTGGGCAGATGCTAGGATGTTGATTGATTACAATTATTTTGGTGATGTTGTTTCTCTTGATTCAACATATTGCACAAATAGTTCACACAGACCACTAGCAATCATCTCGGGATTTAACCACCATCGAGGAGCTGTAATTTTTGGAGCTGCATTATTATATGATGAAACTGCTGAGTCATACGAGTGGCTCTTCGAGACATTTCTAGAAGCACATAAGCAGAAAATGCCTCAAACAGTTTTTACTGATCAAGACAAATCAATGGCAAAAGCATTAGCCGAGGTTAATGCCCGGAGCTTACCATGGTTTATGTACGTGGCACTTGATGCAAAATGGAATTAAACACTTAGGAAATTTAATGAAGGGGGAATCTTATTTTCTTACTGGCTTTAAGAAATGCATGTATGGTTATGAAGATGAAGAACAATTTGAAGATGGTTGGACATCCCTCCTTAAAAAGTATGGTGTTCAGAAAAATGATTGGTTGCAAAGAATGTACAATATAAAGGAGAAATGGGCATCTTGTTACATGAAGAAGACTTTGACATTAGGGATGCGAAGCACTCAACTTAGTGAAAGCGTGAATGCAGATATTAAAAATTTTATGAGTAAGGACTTGGATTTAATTAAATTTTTTGAGCGTTTTGAAGATGTTGTAGAAGAAAAGCGGTATAAAGAATTGAAGTGTGAATTTGAAGCATGCTTTGTGAAAAGCATGGACATACAAACATCATCATCTACTTATGTTATTGCCAAGAAAAGGAATATGGGAGAATGGCAAGTGACCTTCGATTTGGAAAAGAATACAATTTGTTGCTCTTGTCGTAAATTTGAGAGCTTTGGCATACTTTGTTGTCATTGCTTAAAAGTCTTCATTCATATGGACATTACTTCAGTGCCTGAGCCTTATATTTTAAAGAGGTGGACAAATATAGCAAGAAGTAGAGCTTCACAAACTATTGGTGTTAGTCATGTGGTAGAAGACTTGGATTTATCTCGAGCACAATGTTATAAGGAAATTTGTCCCCGCTTTATTAGGATCGTCACAGAAGGATGTAGAAGTCCAGAAGCTGTCGAGTTTCTTAAGGAAGTTGTTGATGAATTGGATGAGCAAATGTTGAAGTTTCAAAATATGCAAATAAGTAGTGAACAAGTGAATGGAACCTCTAACAATATTAAAGAGATATCAAGTACTAATGATGGTGCAACACAAGTAAAGGGGTTTAAGAACAAAGAAGGTCCAAAGGGTTCAAAACGTTGGAAAGGTTGGGTACAAACGAAACTGGCCAAGAGAAAGAAACGTGCAAAGTCCACTGCTTCACAAATTCAAGAACCTATGGTATTATTAATATATTTAGTTTTATTTTTTGTTTTTGTTGACTACATAAGCTCACCTTTATATTCCTTTTTTTTTGTGTAGAAGAACAAAGGTAAGCAAGGTTCCAAAAATATAAAGAGGCGGGATGAAGTGTCAAACACAGGTGCTTCACAAAGTCAAAAGCAATATGTTGTGTCCAACATTAGTGATTTACAATGTCAACAGCCATATGCATCGCCAATTACAAGTGCACCTATATGGCTTAATAATGCATACAATCAAACCATCTATGGTTAGCTAATAACAATTTGAATTTGTGAATGACCTTAACTATGTTTAGTGTTTAAATTATTTTGGTTTTTATGTAGGGAGACGATGGAACATCTAGTCAATTTGGTGTCGCTAGTTTTACTAGCTTATTGATGGTAAATTTCAAATTATACGATTCACTTTCAAATCTATATATATAATATTAACTTGCATTTTTTAAATTATAGGAGCAACTTGATGATGGTACCAGAAACTTGAATGGAGGCCTGTAG
Protein sequence

>MS.gene40559.t1

MNEDWKPKVDMVFGSFEDAWEFWKTYGGKVGFGVRKDYTHRKKDGSVSSCRFVCCKEGIRKPDKQDHKTKNPRLETRTNCEARLGLKNVDGKLMVHDFVEDHNHELRLPETTHMMSSQQKVSEIQCQQIELADSAGVQQRKSFDLLSKEVGGRTNSGFIRLDQKNYLRKKRERSLVQGEAGYLLQYFQRKSVENPTFYHAYQLDIEDQITNGESYFLTGFKKCMYGYEDEEQFEDGWTSLLKKYGVQKNDWLQRMYNIKEKWASCYMKKTLTLGMRSTQLSESVNADIKNFMSKDLDLIKFFERFEDVVEEKRYKELKCEFEACFVKSMDIQTSSSTYVIAKKRNMGEWQVTFDLEKNTICCSCRKFESFGILCCHCLKVFIHMDITSVPEPYILKRWTNIARSRASQTIGVSHVVEDLDLSRAQCYKEICPRFIRIVTEGCRSPEAVEFLKEVVDELDEQMLKFQNMQISSEQVNGTSNNIKEISSTNDGATQVKGFKNKEGPKGSKRWKGWVQTKLAKRKKRAKSTASQIQEPMKNKGKQGSKNIKRRDEVSNTGASQSQKQYVVSNISDLQCQQPYASPITSAPIWLNNAYNQTIYDDGTSSQFGVASFTSLLMEQLDDGTRNLNGGL