AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40727.t1 MTR_3g109760 92.025 326 15 4 1 323 1 318 0.0 543
MS.gene40727.t1 MTR_1g006650 52.571 350 121 10 6 323 8 344 5.36e-106 313
MS.gene40727.t1 MTR_8g013950 44.809 366 135 14 1 323 1 342 2.15e-79 245
MS.gene40727.t1 MTR_4g071590 36.824 296 144 11 1 281 13 280 6.59e-43 150
MS.gene40727.t1 MTR_4g471590 36.824 296 144 11 1 281 13 280 6.59e-43 150
MS.gene40727.t1 MTR_8g076340 39.362 282 128 11 19 282 32 288 1.31e-41 146
MS.gene40727.t1 MTR_3g117380 39.852 271 142 10 19 282 43 299 2.99e-39 140
MS.gene40727.t1 MTR_1g094930 37.597 258 97 8 83 276 49 306 4.21e-37 135
MS.gene40727.t1 MTR_6g032830 37.054 224 92 5 104 278 35 258 6.00e-36 130
MS.gene40727.t1 MTR_7g085410 41.038 212 82 7 104 276 45 252 1.17e-34 127
MS.gene40727.t1 MTR_5g021340 40.708 226 96 7 90 282 150 370 1.70e-34 130
MS.gene40727.t1 MTR_1g094930 57.009 107 44 2 171 276 106 211 3.85e-34 125
MS.gene40727.t1 MTR_1g046680 78.873 71 15 0 211 281 171 241 4.85e-33 122
MS.gene40727.t1 MTR_5g007600 53.211 109 51 0 173 281 220 328 9.27e-33 124
MS.gene40727.t1 MTR_5g007600 49.206 126 49 2 151 275 109 220 1.07e-29 115
MS.gene40727.t1 MTR_1g116010 73.529 68 18 0 209 276 161 228 2.07e-29 113
MS.gene40727.t1 MTR_4g058910 62.963 81 30 0 212 292 314 394 9.52e-29 114
MS.gene40727.t1 MTR_2g094600 75.385 65 16 0 212 276 216 280 9.86e-29 112
MS.gene40727.t1 MTR_7g446110 54.430 79 33 2 201 278 175 251 1.56e-21 92.0
MS.gene40727.t1 MTR_7g446070 34.239 184 103 6 91 272 25 192 1.95e-20 87.8
MS.gene40727.t1 MTR_7g445950 33.702 181 87 6 95 272 30 180 1.15e-19 85.5
MS.gene40727.t1 MTR_7g445980 64.286 56 19 1 216 270 37 92 1.25e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40727.t1 AT4G32890 49.367 316 115 10 30 323 16 308 2.97e-86 262
MS.gene40727.t1 AT5G25830 46.893 354 130 11 2 323 4 331 7.41e-85 259
MS.gene40727.t1 AT3G60530 48.039 204 74 5 89 292 63 234 1.69e-51 170
MS.gene40727.t1 AT2G45050 47.907 215 76 7 89 289 65 257 1.39e-48 164
MS.gene40727.t1 AT4G36240 39.300 257 111 8 32 279 11 231 7.10e-38 135
MS.gene40727.t1 AT5G66320 39.768 259 98 7 76 292 87 329 1.60e-37 137
MS.gene40727.t1 AT5G66320 39.768 259 98 7 76 292 87 329 1.60e-37 137
MS.gene40727.t1 AT3G24050 77.941 68 15 0 211 278 193 260 7.07e-33 123
MS.gene40727.t1 AT1G08010 65.854 82 22 1 209 284 217 298 1.88e-31 120
MS.gene40727.t1 AT1G08010 65.854 82 22 1 209 284 217 298 1.88e-31 120
MS.gene40727.t1 AT1G08010 65.854 82 22 1 209 284 217 298 1.88e-31 120
MS.gene40727.t1 AT3G54810 75.000 68 17 0 212 279 229 296 2.75e-31 120
MS.gene40727.t1 AT3G54810 75.000 68 17 0 212 279 229 296 2.75e-31 120
MS.gene40727.t1 AT4G34680 42.935 184 74 7 99 281 96 249 3.44e-31 118
MS.gene40727.t1 AT4G34680 42.935 184 74 7 99 281 96 249 3.44e-31 118
MS.gene40727.t1 AT3G51080 73.913 69 18 0 212 280 221 289 1.07e-29 115
MS.gene40727.t1 AT1G08000 59.091 88 31 1 209 291 215 302 1.92e-29 114
MS.gene40727.t1 AT1G08000 59.091 88 31 1 209 291 215 302 1.92e-29 114
MS.gene40727.t1 AT3G45170 60.870 69 27 0 212 280 115 183 3.05e-25 100
MS.gene40727.t1 AT2G28340 66.667 63 21 0 214 276 193 255 5.72e-24 99.8

Find 92 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGATTCCTCAGCAAATT+TGG 0.139979 3.2:+88160261 None:intergenic
CTACATGAGGTGCGTGAAAT+CGG 0.267854 3.2:+88159634 None:intergenic
CACGCATTGCATAGTGTCTT+TGG 0.290696 3.2:+88159890 None:intergenic
ACAGTTGATATCTGGATTAA+AGG 0.306992 3.2:-88160215 MS.gene40727:CDS
CATAAGCTACAGTTGATATC+TGG 0.309428 3.2:-88160223 MS.gene40727:CDS
AAGGGCCGTCGGCAGAATTC+TGG 0.309787 3.2:+88161852 None:intergenic
CAGTGGCGCACCGGGCCATT+AGG 0.332794 3.2:-88159914 MS.gene40727:CDS
CATAGTGTCTTTGGACCTAA+TGG 0.339675 3.2:+88159899 None:intergenic
CACGTGTGCCTCCATGCAAC+TGG 0.370780 3.2:-88160099 MS.gene40727:CDS
GGGCCCTCATCATCTCCTTC+TGG 0.374473 3.2:+88159753 None:intergenic
ATTGTGGACAGAAGCTGGTT+TGG 0.397002 3.2:+88162074 None:intergenic
TTGCAACAATTGGTGTTGTT+GGG 0.408578 3.2:+88159733 None:intergenic
GTTGCAACAATTGGTGTTGT+TGG 0.409369 3.2:+88159732 None:intergenic
GACACTTGAGGGTGTATTGT+TGG 0.425894 3.2:+88160151 None:intergenic
TGCTTAGTCAACACAAATGT+TGG 0.431309 3.2:+88159812 None:intergenic
CATCACAGCATTATGTTTGA+AGG 0.433831 3.2:-88159701 MS.gene40727:CDS
TCTCTTTGATTTCTCTAATG+AGG 0.434233 3.2:-88161987 MS.gene40727:CDS
CGGCAAAAGCCCGTAGTAAA+CGG 0.443499 3.2:-88160123 MS.gene40727:CDS
TTCGAAAGTGGGGTCTTCAA+TGG 0.445894 3.2:+88161958 None:intergenic
TACATGAGGTGCGTGAAATC+GGG 0.448275 3.2:+88159635 None:intergenic
TTGCACCTTTCGATGTGAGT+TGG 0.456005 3.2:+88159787 None:intergenic
CTCCGGCGAACTCTCCGTTC+CGG 0.462198 3.2:-88161828 MS.gene40727:intron
CCACTGTGGAGTCTTATCAT+TGG 0.462705 3.2:+88159931 None:intergenic
ACGGAGAGTTCGCCGGAGAA+AGG 0.464710 3.2:+88161833 None:intergenic
AGATGTGGTGGTGGTGGTCA+TGG 0.465812 3.2:+88160048 None:intergenic
GTTTGTGGTGGAATTGTTGT+CGG 0.468374 3.2:+88162051 None:intergenic
ACAAATGTTGGGCTTGCTGC+AGG 0.469450 3.2:+88159824 None:intergenic
GTGGTGGTGGTGGTCATGGT+TGG 0.470501 3.2:+88160052 None:intergenic
TTGCCGGGACAGACACTTGA+GGG 0.471708 3.2:+88160140 None:intergenic
ATGATCTTGCGCAGCTAGAA+TGG 0.476849 3.2:-88160288 MS.gene40727:CDS
TCATCATCTCCTTCTGGCGT+CGG 0.486176 3.2:+88159759 None:intergenic
CGGAGAGTTCGCCGGAGAAA+GGG 0.487872 3.2:+88161834 None:intergenic
GCTTAGTCAACACAAATGTT+GGG 0.498811 3.2:+88159813 None:intergenic
AGCAGTGTTTACATTGTTTG+TGG 0.499828 3.2:+88162036 None:intergenic
TCTGCCGACGGCCCTTTCTC+CGG 0.501462 3.2:-88161845 MS.gene40727:CDS
GTGGGATTGGTTTCAAGAGG+AGG 0.506926 3.2:+88161905 None:intergenic
GTGGAATTGTTGTCGGATTG+TGG 0.509048 3.2:+88162058 None:intergenic
AGGAGGAGATTCTTCGAAAG+TGG 0.514544 3.2:+88161946 None:intergenic
GATGTTGTAGTTGCAACAAT+TGG 0.517809 3.2:+88159723 None:intergenic
ACACTTGAGGGTGTATTGTT+GGG 0.518688 3.2:+88160152 None:intergenic
AGACTCCACAGTGGCGCACC+GGG 0.518894 3.2:-88159922 MS.gene40727:CDS
AAGACTCCACAGTGGCGCAC+CGG 0.525177 3.2:-88159923 MS.gene40727:CDS
GAATCATTAGAGTTGGTAGG+AGG 0.531077 3.2:+88161929 None:intergenic
GCAAGAGCTCCGACGCCAGA+AGG 0.533988 3.2:-88159768 MS.gene40727:CDS
GTCGGATTGTGGACAGAAGC+TGG 0.535564 3.2:+88162069 None:intergenic
GGAGAATCATTAGAGTTGGT+AGG 0.541668 3.2:+88161926 None:intergenic
ATATCAACTGTAGCTTATGA+AGG 0.545098 3.2:+88160227 None:intergenic
AGGAGGAGAATCATTAGAGT+TGG 0.548174 3.2:+88161922 None:intergenic
CACACGTGACCGTTTACTAC+GGG 0.563431 3.2:+88160114 None:intergenic
AATGGCCCGGTGCGCCACTG+TGG 0.564107 3.2:+88159917 None:intergenic
GGAGGAGATTCTTCGAAAGT+GGG 0.565310 3.2:+88161947 None:intergenic
TGTGGAGTCAGATACAAGTC+AGG 0.567755 3.2:-88159869 MS.gene40727:CDS
TACCGGAACGGAGAGTTCGC+CGG 0.569597 3.2:+88161826 None:intergenic
GACGCCAGAAGGAGATGATG+AGG 0.570929 3.2:-88159757 MS.gene40727:CDS
GAAGTGGGATTGGTTTCAAG+AGG 0.577002 3.2:+88161902 None:intergenic
AGAGATCTTCAACGATGAAG+TGG 0.579783 3.2:+88162005 None:intergenic
GTGAGAAGATGTGGTGGTGG+TGG 0.580310 3.2:+88160042 None:intergenic
GCACACGTGACCGTTTACTA+CGG 0.581455 3.2:+88160113 None:intergenic
AGGCGCGAAGTCCAGTTGCA+TGG 0.582876 3.2:+88160088 None:intergenic
TGTCTTTGGACCTAATGGCC+CGG 0.587867 3.2:+88159904 None:intergenic
GACCTCGGTGATGGAGGAGA+GGG 0.591558 3.2:-88159974 MS.gene40727:CDS
TTTGCCGGGACAGACACTTG+AGG 0.592935 3.2:+88160139 None:intergenic
ACACCCTCAAGTGTCTGTCC+CGG 0.593472 3.2:-88160143 MS.gene40727:CDS
AAAAGAGACCTCGGTGATGG+AGG 0.595010 3.2:-88159980 MS.gene40727:CDS
AATCAAGTAATCCTCACCGT+TGG 0.595692 3.2:+88159673 None:intergenic
AGAGTGAGAAGATGTGGTGG+TGG 0.596239 3.2:+88160039 None:intergenic
AAGTAATCCTCACCGTTGGA+TGG 0.600786 3.2:+88159677 None:intergenic
TATAATGGCACGTACCGGAA+CGG 0.606350 3.2:+88161814 None:intergenic
ACGCCAGAAGGAGATGATGA+GGG 0.609091 3.2:-88159756 MS.gene40727:CDS
AGACCTCGGTGATGGAGGAG+AGG 0.609530 3.2:-88159975 MS.gene40727:CDS
CTTGCTGCAGGTCTATACTC+AGG 0.609954 3.2:+88159836 None:intergenic
TTCGCCGGAGAAAGGGCCGT+CGG 0.611125 3.2:+88161841 None:intergenic
GCACTCCAACTCACATCGAA+AGG 0.612333 3.2:-88159792 MS.gene40727:CDS
AGTGTTTACATTGTTTGTGG+TGG 0.618078 3.2:+88162039 None:intergenic
ATCTTCAACGATGAAGTGGT+CGG 0.624703 3.2:+88162009 None:intergenic
ATCTTCTCACTCTGACACCA+TGG 0.629511 3.2:-88160029 MS.gene40727:CDS
TCGTGCCAGAATTCTGCCGA+CGG 0.642132 3.2:-88161857 MS.gene40727:CDS
GTCAGAGTGAGAAGATGTGG+TGG 0.644152 3.2:+88160036 None:intergenic
ATGTTTGAAGGACCATCCAA+CGG 0.648434 3.2:-88159689 MS.gene40727:CDS
CCAAAGCACCGTACTACATG+AGG 0.651273 3.2:+88159621 None:intergenic
ATGGATGTCCAAATTTGCTG+AGG 0.653086 3.2:-88160269 MS.gene40727:CDS
TGGTTGGAGAGAGAACTAGA+AGG 0.657111 3.2:+88160068 None:intergenic
CGCGAAGTCCAGTTGCATGG+AGG 0.662375 3.2:+88160091 None:intergenic
TACTTGATTCATCAACACGT+GGG 0.670141 3.2:-88159659 MS.gene40727:CDS
GGTGTCAGAGTGAGAAGATG+TGG 0.671117 3.2:+88160033 None:intergenic
TGAAGGACCATCCAACGGTG+AGG 0.675304 3.2:-88159684 MS.gene40727:CDS
AAGACACTATGCAATGCGTG+TGG 0.676419 3.2:-88159887 MS.gene40727:CDS
GAGGAGATTCTTCGAAAGTG+GGG 0.692052 3.2:+88161948 None:intergenic
TTACTTGATTCATCAACACG+TGG 0.700709 3.2:-88159660 MS.gene40727:CDS
GACCCTCTCCTCCATCACCG+AGG 0.717630 3.2:+88159972 None:intergenic
ATTCTGGCACGAATTGACGT+CGG 0.752925 3.2:+88161868 None:intergenic
CCAATGATAAGACTCCACAG+TGG 0.803218 3.2:-88159931 MS.gene40727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATAGTTAAAAAAAATAT+AGG + chr3.2:88160873-88160892 None:intergenic 10.0%
!! CTATAGTTAAAAAAAATATA+GGG + chr3.2:88160872-88160891 None:intergenic 10.0%
!! TATAGTTAAAAAAAATATAG+GGG + chr3.2:88160871-88160890 None:intergenic 10.0%
!! TATTATTTATATACAACAAT+TGG - chr3.2:88160010-88160029 MS.gene40727:CDS 10.0%
!! ATAGTTAAAAAAAATATAGG+GGG + chr3.2:88160870-88160889 None:intergenic 15.0%
!! ATATAAAGTAAGAATTCTAA+TGG - chr3.2:88161175-88161194 MS.gene40727:intron 15.0%
!! ATATTTGTAATAAAAGCTAA+GGG + chr3.2:88160918-88160937 None:intergenic 15.0%
!! ATGTGACAAAATTAAAAATA+GGG + chr3.2:88160483-88160502 None:intergenic 15.0%
!! TATATTTGTAATAAAAGCTA+AGG + chr3.2:88160919-88160938 None:intergenic 15.0%
!! TATGTGACAAAATTAAAAAT+AGG + chr3.2:88160484-88160503 None:intergenic 15.0%
!!! AAATAAAGAGAGTATTATTT+AGG + chr3.2:88160087-88160106 None:intergenic 15.0%
!!! TATTATTTTTGTTTAGAGAA+GGG + chr3.2:88160957-88160976 None:intergenic 15.0%
!!! TCATTTTTAAGATACTATAT+CGG + chr3.2:88161148-88161167 None:intergenic 15.0%
!!! TCTTAAGATATACTTTTTTT+AGG + chr3.2:88161108-88161127 None:intergenic 15.0%
!!! TTATTATTTTTGTTTAGAGA+AGG + chr3.2:88160958-88160977 None:intergenic 15.0%
!! AGAAAATACATAACCTAATA+TGG - chr3.2:88161327-88161346 MS.gene40727:intron 20.0%
!! CAAAAGTAAAAAAATTGTCT+TGG + chr3.2:88160437-88160456 None:intergenic 20.0%
!! TCTTAAGACAACAATTAAAA+AGG - chr3.2:88161120-88161139 MS.gene40727:intron 20.0%
!! TTTCATAAGTATGACAATTA+TGG + chr3.2:88160276-88160295 None:intergenic 20.0%
!!! ATTAGGTTATGTATTTTCTA+AGG + chr3.2:88161326-88161345 None:intergenic 20.0%
!!! CAAGACAATTTTTTTACTTT+TGG - chr3.2:88160435-88160454 MS.gene40727:intron 20.0%
!!! TTCTTTTTATACTCCATATT+AGG + chr3.2:88161343-88161362 None:intergenic 20.0%
! AATGGAACAGATAAAATTGT+AGG + chr3.2:88160050-88160069 None:intergenic 25.0%
! ATAAGAAAATGTGAGGTATA+TGG - chr3.2:88160579-88160598 MS.gene40727:intron 25.0%
! TAAGAAAATGTGAGGTATAT+GGG - chr3.2:88160580-88160599 MS.gene40727:intron 25.0%
!! GAGTGAATTTTGAATTTCAA+GGG - chr3.2:88160610-88160629 MS.gene40727:intron 25.0%
!!! ATAGTCTTTTATTTAGTCGT+GGG - chr3.2:88160554-88160573 MS.gene40727:intron 25.0%
!!! GTTTTTGATTCAACGTTATT+TGG + chr3.2:88161541-88161560 None:intergenic 25.0%
!!! TATAGTCTTTTATTTAGTCG+TGG - chr3.2:88160553-88160572 MS.gene40727:intron 25.0%
AAATGATTCCTCAGCAAATT+TGG + chr3.2:88161470-88161489 None:intergenic 30.0%
ATATATATAATGGCACGTAC+CGG + chr3.2:88159922-88159941 None:intergenic 30.0%
ATATCAACTGTAGCTTATGA+AGG + chr3.2:88161504-88161523 None:intergenic 30.0%
ATCTGTTCCATTGAATTTGA+GGG - chr3.2:88160058-88160077 MS.gene40727:CDS 30.0%
CACACATATTAAAGAAAAGC+AGG + chr3.2:88160198-88160217 None:intergenic 30.0%
TATCTGTTCCATTGAATTTG+AGG - chr3.2:88160057-88160076 MS.gene40727:CDS 30.0%
! ACAGTTGATATCTGGATTAA+AGG - chr3.2:88161513-88161532 MS.gene40727:intron 30.0%
! GGAGTGAATTTTGAATTTCA+AGG - chr3.2:88160609-88160628 MS.gene40727:intron 30.0%
! TCTCTTTGATTTCTCTAATG+AGG - chr3.2:88159741-88159760 MS.gene40727:CDS 30.0%
AAAATTAAAAATAGGGCCCC+GGG + chr3.2:88160476-88160495 None:intergenic 35.0%
AGCAGTGTTTACATTGTTTG+TGG + chr3.2:88159695-88159714 None:intergenic 35.0%
AGTGTTTACATTGTTTGTGG+TGG + chr3.2:88159692-88159711 None:intergenic 35.0%
ATTGACGTCGGTGAAAAAAT+TGG + chr3.2:88159851-88159870 None:intergenic 35.0%
ATTGGTTAAGCAGCTAAAAG+AGG - chr3.2:88160352-88160371 MS.gene40727:intron 35.0%
CAAAATTAAAAATAGGGCCC+CGG + chr3.2:88160477-88160496 None:intergenic 35.0%
CATCACAGCATTATGTTTGA+AGG - chr3.2:88162027-88162046 MS.gene40727:CDS 35.0%
GAAAAAATTGGAAGTGGGAT+TGG + chr3.2:88159839-88159858 None:intergenic 35.0%
GATGTTGTAGTTGCAACAAT+TGG + chr3.2:88162008-88162027 None:intergenic 35.0%
GCTTAGTCAACACAAATGTT+GGG + chr3.2:88161918-88161937 None:intergenic 35.0%
GTGGGAAATAAGAAAATGTG+AGG - chr3.2:88160572-88160591 MS.gene40727:intron 35.0%
TACTTGATTCATCAACACGT+GGG - chr3.2:88162069-88162088 MS.gene40727:CDS 35.0%
TAGGTTTCCCTCAAATTCAA+TGG + chr3.2:88160068-88160087 None:intergenic 35.0%
TCGGTGAAAAAATTGGAAGT+GGG + chr3.2:88159844-88159863 None:intergenic 35.0%
TGCTTAGTCAACACAAATGT+TGG + chr3.2:88161919-88161938 None:intergenic 35.0%
TTACTTGATTCATCAACACG+TGG - chr3.2:88162068-88162087 MS.gene40727:CDS 35.0%
TTGACTCAACATGTGTAGTT+AGG - chr3.2:88159976-88159995 MS.gene40727:CDS 35.0%
! CATAAGCTACAGTTGATATC+TGG - chr3.2:88161505-88161524 MS.gene40727:intron 35.0%
!! TTGCAACAATTGGTGTTGTT+GGG + chr3.2:88161998-88162017 None:intergenic 35.0%
!!! TGAGGAATCATTTTCAAGTG+AGG - chr3.2:88161477-88161496 MS.gene40727:intron 35.0%
AAATTAAAAATAGGGCCCCG+GGG + chr3.2:88160475-88160494 None:intergenic 40.0%
AATCAAGTAATCCTCACCGT+TGG + chr3.2:88162058-88162077 None:intergenic 40.0%
AGAGATCTTCAACGATGAAG+TGG + chr3.2:88159726-88159745 None:intergenic 40.0%
AGGAGGAGAATCATTAGAGT+TGG + chr3.2:88159809-88159828 None:intergenic 40.0%
ATATCTTATGTTCGCCGTGT+AGG + chr3.2:88161285-88161304 None:intergenic 40.0%
ATCTTCAACGATGAAGTGGT+CGG + chr3.2:88159722-88159741 None:intergenic 40.0%
ATGTGAGGTATATGGGATAG+AGG - chr3.2:88160587-88160606 MS.gene40727:intron 40.0%
ATGTTTGAAGGACCATCCAA+CGG - chr3.2:88162039-88162058 MS.gene40727:CDS 40.0%
CATAGTGTCTTTGGACCTAA+TGG + chr3.2:88161832-88161851 None:intergenic 40.0%
CCTTATGATACACACCTACA+CGG - chr3.2:88161268-88161287 MS.gene40727:intron 40.0%
GTCGGTGAAAAAATTGGAAG+TGG + chr3.2:88159845-88159864 None:intergenic 40.0%
TCTCCAAGAAAAAGAGACCT+CGG - chr3.2:88161739-88161758 MS.gene40727:intron 40.0%
TGTGAGGTATATGGGATAGA+GGG - chr3.2:88160588-88160607 MS.gene40727:intron 40.0%
! ACACTTGAGGGTGTATTGTT+GGG + chr3.2:88161579-88161598 None:intergenic 40.0%
! AGGTCTCTTTTTCTTGGAGA+GGG + chr3.2:88161739-88161758 None:intergenic 40.0%
! GGAGAATCATTAGAGTTGGT+AGG + chr3.2:88159805-88159824 None:intergenic 40.0%
! GTTTGTGGTGGAATTGTTGT+CGG + chr3.2:88159680-88159699 None:intergenic 40.0%
!! ATGGATGTCCAAATTTGCTG+AGG - chr3.2:88161459-88161478 MS.gene40727:intron 40.0%
!! GAATCATTAGAGTTGGTAGG+AGG + chr3.2:88159802-88159821 None:intergenic 40.0%
!! GTTGCAACAATTGGTGTTGT+TGG + chr3.2:88161999-88162018 None:intergenic 40.0%
!!! TTTCTTGGAGAGGGCTTTTT+TGG + chr3.2:88161730-88161749 None:intergenic 40.0%
AAGACACTATGCAATGCGTG+TGG - chr3.2:88161841-88161860 MS.gene40727:CDS 45.0%
AAGTAATCCTCACCGTTGGA+TGG + chr3.2:88162054-88162073 None:intergenic 45.0%
AGAAAAAGAGACCTCGGTGA+TGG - chr3.2:88161745-88161764 MS.gene40727:intron 45.0%
AGGAGGAGATTCTTCGAAAG+TGG + chr3.2:88159785-88159804 None:intergenic 45.0%
ATCTTCTCACTCTGACACCA+TGG - chr3.2:88161699-88161718 MS.gene40727:intron 45.0%
ATGATCTTGCGCAGCTAGAA+TGG - chr3.2:88161440-88161459 MS.gene40727:intron 45.0%
ATTCTGGCACGAATTGACGT+CGG + chr3.2:88159863-88159882 None:intergenic 45.0%
ATTGTGGACAGAAGCTGGTT+TGG + chr3.2:88159657-88159676 None:intergenic 45.0%
CACGCATTGCATAGTGTCTT+TGG + chr3.2:88161841-88161860 None:intergenic 45.0%
CCAATGATAAGACTCCACAG+TGG - chr3.2:88161797-88161816 MS.gene40727:intron 45.0%
CCGTGTAGGTGTGTATCATA+AGG + chr3.2:88161271-88161290 None:intergenic 45.0%
CTACATGAGGTGCGTGAAAT+CGG + chr3.2:88162097-88162116 None:intergenic 45.0%
GAGGAGATTCTTCGAAAGTG+GGG + chr3.2:88159783-88159802 None:intergenic 45.0%
GGAGGAGATTCTTCGAAAGT+GGG + chr3.2:88159784-88159803 None:intergenic 45.0%
GTGGAATTGTTGTCGGATTG+TGG + chr3.2:88159673-88159692 None:intergenic 45.0%
TACATGAGGTGCGTGAAATC+GGG + chr3.2:88162096-88162115 None:intergenic 45.0%
TATAATGGCACGTACCGGAA+CGG + chr3.2:88159917-88159936 None:intergenic 45.0%
TGGATTCTTAACAAGTGCCC+CGG - chr3.2:88160455-88160474 MS.gene40727:intron 45.0%
TGGTTGGAGAGAGAACTAGA+AGG + chr3.2:88161663-88161682 None:intergenic 45.0%
TGTGGAGTCAGATACAAGTC+AGG - chr3.2:88161859-88161878 MS.gene40727:CDS 45.0%
TTCGAAAGTGGGGTCTTCAA+TGG + chr3.2:88159773-88159792 None:intergenic 45.0%
TTGCACCTTTCGATGTGAGT+TGG + chr3.2:88161944-88161963 None:intergenic 45.0%
! CCACTGTGGAGTCTTATCAT+TGG + chr3.2:88161800-88161819 None:intergenic 45.0%
! GACACTTGAGGGTGTATTGT+TGG + chr3.2:88161580-88161599 None:intergenic 45.0%
! GAGGTCTCTTTTTCTTGGAG+AGG + chr3.2:88161740-88161759 None:intergenic 45.0%
! TCACCGAGGTCTCTTTTTCT+TGG + chr3.2:88161745-88161764 None:intergenic 45.0%
!! GAAGTGGGATTGGTTTCAAG+AGG + chr3.2:88159829-88159848 None:intergenic 45.0%
!! AATAATTACTATATATATAA+TGG + chr3.2:88159932-88159951 None:intergenic 5.0%
!! TATAAATATATGATAAAAAA+AGG + chr3.2:88160537-88160556 None:intergenic 5.0%
!!! ATAAATTTTATTATATTTGA+TGG + chr3.2:88161042-88161061 None:intergenic 5.0%
!!! TAAAATGAATATTATTTTTA+AGG - chr3.2:88160303-88160322 MS.gene40727:intron 5.0%
ACGCCAGAAGGAGATGATGA+GGG - chr3.2:88161972-88161991 MS.gene40727:CDS 50.0%
AGAATGTTAACCAGTGCCCC+CGG - chr3.2:88160326-88160345 MS.gene40727:intron 50.0%
AGAGTGAGAAGATGTGGTGG+TGG + chr3.2:88161692-88161711 None:intergenic 50.0%
CACACGTGACCGTTTACTAC+GGG + chr3.2:88161617-88161636 None:intergenic 50.0%
CGGCAAAAGCCCGTAGTAAA+CGG - chr3.2:88161605-88161624 MS.gene40727:intron 50.0%
CTTGCTGCAGGTCTATACTC+AGG + chr3.2:88161895-88161914 None:intergenic 50.0%
GATTCTTAACAAGTGCCCCG+GGG - chr3.2:88160457-88160476 MS.gene40727:intron 50.0%
GCACACGTGACCGTTTACTA+CGG + chr3.2:88161618-88161637 None:intergenic 50.0%
GCACTCCAACTCACATCGAA+AGG - chr3.2:88161936-88161955 MS.gene40727:CDS 50.0%
GGATTCTTAACAAGTGCCCC+GGG - chr3.2:88160456-88160475 MS.gene40727:intron 50.0%
GGTGTCAGAGTGAGAAGATG+TGG + chr3.2:88161698-88161717 None:intergenic 50.0%
GTCAGAGTGAGAAGATGTGG+TGG + chr3.2:88161695-88161714 None:intergenic 50.0%
TAGCTGCTTAACCAATGCCC+CGG + chr3.2:88160348-88160367 None:intergenic 50.0%
TCATCATCTCCTTCTGGCGT+CGG + chr3.2:88161972-88161991 None:intergenic 50.0%
TGTCTTTGGACCTAATGGCC+CGG + chr3.2:88161827-88161846 None:intergenic 50.0%
! AAAAGAGACCTCGGTGATGG+AGG - chr3.2:88161748-88161767 MS.gene40727:intron 50.0%
! CGTTTACTACGGGCTTTTGC+CGG + chr3.2:88161607-88161626 None:intergenic 50.0%
! GTTTACTACGGGCTTTTGCC+GGG + chr3.2:88161606-88161625 None:intergenic 50.0%
!! ACAAATGTTGGGCTTGCTGC+AGG + chr3.2:88161907-88161926 None:intergenic 50.0%
!! GTGGGATTGGTTTCAAGAGG+AGG + chr3.2:88159826-88159845 None:intergenic 50.0%
!!! CTTGGAGAGGGCTTTTTTGG+TGG + chr3.2:88161727-88161746 None:intergenic 50.0%
AATGTTAACCAGTGCCCCCG+GGG - chr3.2:88160328-88160347 MS.gene40727:intron 55.0%
ACACCCTCAAGTGTCTGTCC+CGG - chr3.2:88161585-88161604 MS.gene40727:intron 55.0%
AGCTGCTTAACCAATGCCCC+GGG + chr3.2:88160347-88160366 None:intergenic 55.0%
GAATGTTAACCAGTGCCCCC+GGG - chr3.2:88160327-88160346 MS.gene40727:intron 55.0%
GACGCCAGAAGGAGATGATG+AGG - chr3.2:88161971-88161990 MS.gene40727:CDS 55.0%
GTCGGATTGTGGACAGAAGC+TGG + chr3.2:88159662-88159681 None:intergenic 55.0%
TCGTGCCAGAATTCTGCCGA+CGG - chr3.2:88159871-88159890 MS.gene40727:CDS 55.0%
TGAAGGACCATCCAACGGTG+AGG - chr3.2:88162044-88162063 MS.gene40727:CDS 55.0%
TTGCCGGGACAGACACTTGA+GGG + chr3.2:88161591-88161610 None:intergenic 55.0%
TTTGCCGGGACAGACACTTG+AGG + chr3.2:88161592-88161611 None:intergenic 55.0%
!! AGATGTGGTGGTGGTGGTCA+TGG + chr3.2:88161683-88161702 None:intergenic 55.0%
!! GTGAGAAGATGTGGTGGTGG+TGG + chr3.2:88161689-88161708 None:intergenic 55.0%
!!! GGGCTTTTTTGGTGGAGCCA+TGG + chr3.2:88161719-88161738 None:intergenic 55.0%
AAGACTCCACAGTGGCGCAC+CGG - chr3.2:88161805-88161824 MS.gene40727:intron 60.0%
AAGGGCCGTCGGCAGAATTC+TGG + chr3.2:88159879-88159898 None:intergenic 60.0%
ACGGAGAGTTCGCCGGAGAA+AGG + chr3.2:88159898-88159917 None:intergenic 60.0%
AGGCGCGAAGTCCAGTTGCA+TGG + chr3.2:88161643-88161662 None:intergenic 60.0%
CACGTGTGCCTCCATGCAAC+TGG - chr3.2:88161629-88161648 MS.gene40727:intron 60.0%
CGCGAAGTCCAGTTGCATGG+AGG + chr3.2:88161640-88161659 None:intergenic 60.0%
CGGAGAGTTCGCCGGAGAAA+GGG + chr3.2:88159897-88159916 None:intergenic 60.0%
CTGCTTAACCAATGCCCCGG+GGG + chr3.2:88160345-88160364 None:intergenic 60.0%
GCTGCTTAACCAATGCCCCG+GGG + chr3.2:88160346-88160365 None:intergenic 60.0%
GGGCCCTCATCATCTCCTTC+TGG + chr3.2:88161978-88161997 None:intergenic 60.0%
TACCGGAACGGAGAGTTCGC+CGG + chr3.2:88159905-88159924 None:intergenic 60.0%
!! AGACCTCGGTGATGGAGGAG+AGG - chr3.2:88161753-88161772 MS.gene40727:intron 60.0%
!! GACCTCGGTGATGGAGGAGA+GGG - chr3.2:88161754-88161773 MS.gene40727:intron 60.0%
!! GTGGTGGTGGTGGTCATGGT+TGG + chr3.2:88161679-88161698 None:intergenic 60.0%
AGACTCCACAGTGGCGCACC+GGG - chr3.2:88161806-88161825 MS.gene40727:intron 65.0%
CTCCGGCGAACTCTCCGTTC+CGG - chr3.2:88159900-88159919 MS.gene40727:CDS 65.0%
GACCCTCTCCTCCATCACCG+AGG + chr3.2:88161759-88161778 None:intergenic 65.0%
GCAAGAGCTCCGACGCCAGA+AGG - chr3.2:88161960-88161979 MS.gene40727:CDS 65.0%
TCTGCCGACGGCCCTTTCTC+CGG - chr3.2:88159883-88159902 MS.gene40727:CDS 65.0%
TTCGCCGGAGAAAGGGCCGT+CGG + chr3.2:88159890-88159909 None:intergenic 65.0%
AACCAGTGCCCCCGGGGCAT+TGG - chr3.2:88160334-88160353 MS.gene40727:intron 70.0%
AATGGCCCGGTGCGCCACTG+TGG + chr3.2:88161814-88161833 None:intergenic 70.0%
CAGTGGCGCACCGGGCCATT+AGG - chr3.2:88161814-88161833 MS.gene40727:intron 70.0%
! AACCAATGCCCCGGGGGCAC+TGG + chr3.2:88160339-88160358 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 88159634 88162116 88159634 ID=MS.gene40727
chr3.2 mRNA 88159634 88162116 88159634 ID=MS.gene40727.t1;Parent=MS.gene40727
chr3.2 exon 88161829 88162116 88161829 ID=MS.gene40727.t1.exon1;Parent=MS.gene40727.t1
chr3.2 CDS 88161829 88162116 88161829 ID=cds.MS.gene40727.t1;Parent=MS.gene40727.t1
chr3.2 exon 88159634 88160317 88159634 ID=MS.gene40727.t1.exon2;Parent=MS.gene40727.t1
chr3.2 CDS 88159634 88160317 88159634 ID=cds.MS.gene40727.t1;Parent=MS.gene40727.t1
Gene Sequence

>MS.gene40727

ATGGAAGCTCAAGAGTTTTTCCAAACCAGCTTCTGTCCACAATCCGACAACAATTCCACCACAAACAATGTAAACACTGCTGCTTCCGACCACTTCATCGTTGAAGATCTCTTTGATTTCTCTAATGAGGATGTTGCCATTGAAGACCCCACTTTCGAAGAATCTCCTCCTACCAACTCTAATGATTCTCCTCCTCTTGAAACCAATCCCACTTCCAATTTTTTCACCGACGTCAATTCGTGCCAGAATTCTGCCGACGGCCCTTTCTCCGGCGAACTCTCCGTTCCGGTACGTGCCATTATATATATAGTAATTATTGTACATATGTAGTTAGTTTTCTTTTTGACTCAACATGTGTAGTTAGGTAGCTTATTGATATTATTTATATACAACAATTGGCTTGTTTTTACTATCCTACAATTTTATCTGTTCCATTGAATTTGAGGGAAACCTAAATAATACTCTCTTTATTTCACAATATCTGTCACATTTGTAAAAACAATTGTTCAAAAAAATTGATAAGAGCAAACAGTTAATAAAGTTAATTTAGTAAAATCGATACCTGCTTTTCTTTAATATGTGTGAAACTAAAAAACGTGATAGTTATTTTGAAATTGAGTGATATTATTACTCACTCATCCATAATTGTCATACTTATGAAATTTTGTCTAAAATGAATATTATTTTTAAGGAGAATGTTAACCAGTGCCCCCGGGGCATTGGTTAAGCAGCTAAAAGAGGTAAGATTTTATTAAAAAGTTGTGTAATAATTAGTTAGTCAAAAGTATCAGTTTATATTTCCAAGACAATTTTTTTACTTTTGGATTCTTAACAAGTGCCCCGGGGCCCTATTTTTAATTTTGTCACATATTCGATAACGTTGATATAATGAATTATTATCCTTTTTTTATCATATATTTATAGTCTTTTATTTAGTCGTGGGAAATAAGAAAATGTGAGGTATATGGGATAGAGGGAGTGAATTTTGAATTTCAAGGGTTGAATGTAAAGTCAATGAGTAAACAGTTTACTTGTAAATATTTTAATTTTAATCTAATTAATTAGTTAGAAAATATATTACAATGATTAAATGCACACGAAATGAAAATTTTAATTATTTATTTATCATACAAAAATAAATGTACATTTTTTTAAATTATCATTTTATTTTATTCTTTTAAAATAGTACTCTCTCTAAATGTCACATTTATATTTATTTTTTTAAATGATACTCCCCCTATATTTTTTTTAACTATAGTCAAATCTATTTCAATTAAGAAACCCTTAGCTTTTATTACAAATATAAATAAGTGCAATATTCCCTTCTCTAAACAAAAATAATAATAAGTGCAATAAGCTAATAAATAGTTAAGAAAATACTCTAGAAAAATAAAAATAAAACGCTCCATCAAATATAATAAAATTTATTTTTATTCTCATTATGTGTAAATAATCTTAAGATAACACTTTTCCTAAAAAAAGTATATCTTAAGACAACAATTAAAAAGGAACCGATATAGTATCTTAAAAATGATAGTATTATATAAAGTAAGAATTCTAATGGAGACAAAAAAAAAAGAAGTAAGAAGTCTCATAAAAAAATAAAATTATGTAAGAATTAGTTAAATTTTTTTCCTTATGATACACACCTACACGGCGAACATAAGATATTTTTATTTATAGTTTATTCCTTAGAAAATACATAACCTAATATGGAGTATAAAAAGAAAAAAGAAAAAAAAAACATATGTTAATATTTTCTTGTTAATTGACATATTTTAAATTTTTTATTTGTGAAGCAATATGATGATCTTGCGCAGCTAGAATGGATGTCCAAATTTGCTGAGGAATCATTTTCAAGTGAGGACCTTCATAAGCTACAGTTGATATCTGGATTAAAGGCACCAAATAACGTTGAATCAAAAACTCATGAAGAACGCAACCCAACAATACACCCTCAAGTGTCTGTCCCGGCAAAAGCCCGTAGTAAACGGTCACGTGTGCCTCCATGCAACTGGACTTCGCGCCTTCTAGTTCTCTCTCCAACCATGACCACCACCACCACATCTTCTCACTCTGACACCATGGCTCCACCAAAAAAGCCCTCTCCAAGAAAAAGAGACCTCGGTGATGGAGGAGAGGGTCGCAAGTGTCTTCATTGCGCCAATGATAAGACTCCACAGTGGCGCACCGGGCCATTAGGTCCAAAGACACTATGCAATGCGTGTGGAGTCAGATACAAGTCAGGTCGACTCGTACCTGAGTATAGACCTGCAGCAAGCCCAACATTTGTGTTGACTAAGCACTCCAACTCACATCGAAAGGTGCAAGAGCTCCGACGCCAGAAGGAGATGATGAGGGCCCAACAACACCAATTGTTGCAACTACAACATCATCACAGCATTATGTTTGAAGGACCATCCAACGGTGAGGATTACTTGATTCATCAACACGTGGGACCCGATTTCACGCACCTCATGTAG
Protein sequence

>MS.gene40727.t1

MEAQEFFQTSFCPQSDNNSTTNNVNTAASDHFIVEDLFDFSNEDVAIEDPTFEESPPTNSNDSPPLETNPTSNFFTDVNSCQNSADGPFSGELSVPQYDDLAQLEWMSKFAEESFSSEDLHKLQLISGLKAPNNVESKTHEERNPTIHPQVSVPAKARSKRSRVPPCNWTSRLLVLSPTMTTTTTSSHSDTMAPPKKPSPRKRDLGDGGEGRKCLHCANDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVQELRRQKEMMRAQQHQLLQLQHHHSIMFEGPSNGEDYLIHQHVGPDFTHLM