AlfalfaGEDB | MS.gene40753

M. sativa cultivar XinJiangDaYe / MS.gene40753

KEGG: K13152
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF06220.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40753.t1	XP_003591237.1	86.7	128	11	2	1	128	1	122	2.10E-60	241.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40753.t1	Q0JP11	40.2	169	58	3	1	128	1	167	4.9e-29	128.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40753.t1	G7IAA7	86.7	128	11	2	1	128	1	122	1.5e-60	241.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene40753.t1	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40753.t1	MTR_1g084750	85.938	128	12	2	1	128	1	122	9.38e-70	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40753.t1	AT5G26749	39.456	147	70	2	1	128	1	147	2.72e-31	108
MS.gene40753.t1	AT5G26749	34.940	166	70	3	1	128	1	166	9.99e-27	97.8

Find 44 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACCTGGCATTCTTGTTAT+TGG	0.166998	1.2:-59055371	None:intergenic
TGCTGCTGCTGTTGTGTATT+GGG	0.205632	1.2:-59055347	None:intergenic
TACCGGCAGAGTGGCGTTTC+CGG	0.248184	1.2:-59055086	None:intergenic
AGAGTGGCGTTTCCGGTCTA+AGG	0.283984	1.2:-59055079	None:intergenic
TTGCTGCTGCTGTTGTGTAT+TGG	0.317673	1.2:-59055348	None:intergenic
GGAATAGAGTTCTGGTGTTC+GGG	0.348282	1.2:-59055153	None:intergenic
TGGAATAGAGTTCTGGTGTT+CGG	0.377509	1.2:-59055154	None:intergenic
ATCTATCCCCAGTCGACAAA+TGG	0.397656	1.2:-59055582	None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG	0.430967	1.2:-59055161	None:intergenic
GGAATCGTACCAACGAGCTT+TGG	0.434321	1.2:-59055124	None:intergenic
CAACCTCCCATTTGTCGACT+GGG	0.457123	1.2:+59055575	MS.gene40753:CDS
GTCACTGCAGCCTCCGCCTG+AGG	0.458331	1.2:+59055542	MS.gene40753:CDS
TCACTGCAGCCTCCGCCTGA+GGG	0.463350	1.2:+59055543	MS.gene40753:CDS
GTTCTGGTGTTCGGGTTTGA+AGG	0.464430	1.2:-59055145	None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG	0.466779	1.2:-59055180	None:intergenic
GGCTTGTTTGTGTTGAATAC+CGG	0.496651	1.2:-59055103	None:intergenic
TCTATCCCCAGTCGACAAAT+GGG	0.506048	1.2:-59055581	None:intergenic
GATACCATGGGAGTGTCGTT+CGG	0.508317	1.2:+59055507	MS.gene40753:CDS
GACCGGAAACGCCACTCTGC+CGG	0.512557	1.2:+59055084	MS.gene40753:CDS
CCAACCTCCCATTTGTCGAC+TGG	0.514767	1.2:+59055574	MS.gene40753:CDS
TCAGGCGGAGGCTGCAGTGA+CGG	0.518687	1.2:-59055540	None:intergenic
CTGCAGCCTCCGCCTGAGGG+TGG	0.519587	1.2:+59055546	MS.gene40753:CDS
AAGGGTTGATTAGATGGTGG+TGG	0.521880	1.2:-59055174	None:intergenic
TTCAAGATACACCCTTAGAC+CGG	0.529755	1.2:+59055067	MS.gene40753:CDS
GAGTATCCACCCTCAGGCGG+AGG	0.540027	1.2:-59055552	None:intergenic
AGGTTGGAGTATCCACCCTC+AGG	0.543831	1.2:-59055558	None:intergenic
ATATATGTAGGGAATATAGT+AGG	0.551592	1.2:+59055483	MS.gene40753:intron
CCAGTCGACAAATGGGAGGT+TGG	0.561835	1.2:-59055574	None:intergenic
ATAGTACCGTGTTGACGAAG+TGG	0.572113	1.2:-59055203	None:intergenic
CAACCAATAACAAGAATGCC+AGG	0.578455	1.2:+59055368	MS.gene40753:CDS
GAGTGGCGTTTCCGGTCTAA+GGG	0.584406	1.2:-59055078	None:intergenic
TTGACGAAGTGGACGCAGAA+GGG	0.593699	1.2:-59055192	None:intergenic
GTTGACGAAGTGGACGCAGA+AGG	0.597332	1.2:-59055193	None:intergenic
GGCGGAGGCTGCAGTGACGG+AGG	0.613435	1.2:-59055537	None:intergenic
GAATATAGTAGGAGATACCA+TGG	0.619077	1.2:+59055494	MS.gene40753:CDS
CTGCGTCCACTTCGTCAACA+CGG	0.619782	1.2:+59055197	MS.gene40753:CDS
ACAAACAAGCCAAAGCTCGT+TGG	0.650557	1.2:+59055115	MS.gene40753:CDS
ATTGCCGAACGACACTCCCA+TGG	0.650589	1.2:-59055511	None:intergenic
CAGAAGGGTTGATTAGATGG+TGG	0.659317	1.2:-59055177	None:intergenic
TTGGAGTATCCACCCTCAGG+CGG	0.670531	1.2:-59055555	None:intergenic
ATCCCCAGTCGACAAATGGG+AGG	0.671698	1.2:-59055578	None:intergenic
AACCTCCCATTTGTCGACTG+GGG	0.672189	1.2:+59055576	MS.gene40753:CDS
AATATAGTAGGAGATACCAT+GGG	0.697591	1.2:+59055495	MS.gene40753:CDS
TGTGTTGAATACCGGCAGAG+TGG	0.711189	1.2:-59055095	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTATAGTTTCTTTCTTTA+TGG	+	chr1.2:59055421-59055440	MS.gene40753:intron	15.0%
!!	CTAAGAAATGATAATTAATG+AGG	-	chr1.2:59055251-59055270	None:intergenic	20.0%
!	ATATATGTAGGGAATATAGT+AGG	+	chr1.2:59055483-59055502	MS.gene40753:intron	25.0%
!	ATCAGTAAATGAACAACTAT+CGG	-	chr1.2:59055403-59055422	None:intergenic	25.0%
!	ATGTACTATGCATATATGTA+GGG	+	chr1.2:59055472-59055491	MS.gene40753:intron	25.0%
!	TAACTCTCTCTGTATATTTA+GGG	+	chr1.2:59055287-59055306	MS.gene40753:intron	25.0%
!	TATGTACTATGCATATATGT+AGG	+	chr1.2:59055471-59055490	MS.gene40753:intron	25.0%
!	TTAACTCTCTCTGTATATTT+AGG	+	chr1.2:59055286-59055305	MS.gene40753:intron	25.0%
!!!	TCACAATAGTACTTTTTCAT+TGG	-	chr1.2:59055030-59055049	None:intergenic	25.0%
	AATATAGTAGGAGATACCAT+GGG	+	chr1.2:59055495-59055514	MS.gene40753:CDS	30.0%
	TCAGTAAATGAACAACTATC+GGG	-	chr1.2:59055402-59055421	None:intergenic	30.0%
!	ATTTAGGGATTTTGTCGTTA+TGG	+	chr1.2:59055302-59055321	MS.gene40753:intron	30.0%
	CAGTAAATGAACAACTATCG+GGG	-	chr1.2:59055401-59055420	None:intergenic	35.0%
	GAATATAGTAGGAGATACCA+TGG	+	chr1.2:59055494-59055513	MS.gene40753:CDS	35.0%
	CAACCAATAACAAGAATGCC+AGG	+	chr1.2:59055368-59055387	MS.gene40753:CDS	40.0%
	TTCAAGATACACCCTTAGAC+CGG	+	chr1.2:59055067-59055086	MS.gene40753:CDS	40.0%
!	GGCTTGTTTGTGTTGAATAC+CGG	-	chr1.2:59055106-59055125	None:intergenic	40.0%
!	TCACCTGGCATTCTTGTTAT+TGG	-	chr1.2:59055374-59055393	None:intergenic	40.0%
!	TGGAATAGAGTTCTGGTGTT+CGG	-	chr1.2:59055157-59055176	None:intergenic	40.0%
	ACAAACAAGCCAAAGCTCGT+TGG	+	chr1.2:59055115-59055134	MS.gene40753:CDS	45.0%
	TGCTGCTGCTGTTGTGTATT+GGG	-	chr1.2:59055350-59055369	None:intergenic	45.0%
	TTGCTGCTGCTGTTGTGTAT+TGG	-	chr1.2:59055351-59055370	None:intergenic	45.0%
!	AAGGGTTGATTAGATGGTGG+TGG	-	chr1.2:59055177-59055196	None:intergenic	45.0%
!	ACGCAGAAGGGTTGATTAGA+TGG	-	chr1.2:59055183-59055202	None:intergenic	45.0%
!	ATAGTACCGTGTTGACGAAG+TGG	-	chr1.2:59055206-59055225	None:intergenic	45.0%
!	CAGAAGGGTTGATTAGATGG+TGG	-	chr1.2:59055180-59055199	None:intergenic	45.0%
!	GGAATAGAGTTCTGGTGTTC+GGG	-	chr1.2:59055156-59055175	None:intergenic	45.0%
!!	ATGGTGGTGGAATAGAGTTC+TGG	-	chr1.2:59055164-59055183	None:intergenic	45.0%
	GGAATCGTACCAACGAGCTT+TGG	-	chr1.2:59055127-59055146	None:intergenic	50.0%
	TGTGTTGAATACCGGCAGAG+TGG	-	chr1.2:59055098-59055117	None:intergenic	50.0%
	TTGACGAAGTGGACGCAGAA+GGG	-	chr1.2:59055195-59055214	None:intergenic	50.0%
!	AACCTCCCATTTGTCGACTG+GGG	+	chr1.2:59055576-59055595	MS.gene40753:CDS	50.0%
!	CAACCTCCCATTTGTCGACT+GGG	+	chr1.2:59055575-59055594	MS.gene40753:CDS	50.0%
!	GTTCTGGTGTTCGGGTTTGA+AGG	-	chr1.2:59055148-59055167	None:intergenic	50.0%
!!	GATACCATGGGAGTGTCGTT+CGG	+	chr1.2:59055507-59055526	MS.gene40753:CDS	50.0%
	AACTATCGGGGTAGCTCACC+TGG	-	chr1.2:59055389-59055408	None:intergenic	55.0%
	AGAGTGGCGTTTCCGGTCTA+AGG	-	chr1.2:59055082-59055101	None:intergenic	55.0%
	ATCCCCAGTCGACAAATGGG+AGG	-	chr1.2:59055581-59055600	None:intergenic	55.0%
	ATTGCCGAACGACACTCCCA+TGG	-	chr1.2:59055514-59055533	None:intergenic	55.0%
	CCAACCTCCCATTTGTCGAC+TGG	+	chr1.2:59055574-59055593	MS.gene40753:CDS	55.0%
	CCAGTCGACAAATGGGAGGT+TGG	-	chr1.2:59055577-59055596	None:intergenic	55.0%
	CTGCGTCCACTTCGTCAACA+CGG	+	chr1.2:59055197-59055216	MS.gene40753:CDS	55.0%
	GAGTGGCGTTTCCGGTCTAA+GGG	-	chr1.2:59055081-59055100	None:intergenic	55.0%
	GTTGACGAAGTGGACGCAGA+AGG	-	chr1.2:59055196-59055215	None:intergenic	55.0%
!	AGGTTGGAGTATCCACCCTC+AGG	-	chr1.2:59055561-59055580	None:intergenic	55.0%
!	TTGGAGTATCCACCCTCAGG+CGG	-	chr1.2:59055558-59055577	None:intergenic	55.0%
	TACCGGCAGAGTGGCGTTTC+CGG	-	chr1.2:59055089-59055108	None:intergenic	60.0%
	GACCGGAAACGCCACTCTGC+CGG	+	chr1.2:59055084-59055103	MS.gene40753:CDS	65.0%
	GAGTATCCACCCTCAGGCGG+AGG	-	chr1.2:59055555-59055574	None:intergenic	65.0%
	TCACTGCAGCCTCCGCCTGA+GGG	+	chr1.2:59055543-59055562	MS.gene40753:CDS	65.0%
	TCAGGCGGAGGCTGCAGTGA+CGG	-	chr1.2:59055543-59055562	None:intergenic	65.0%
	GTCACTGCAGCCTCCGCCTG+AGG	+	chr1.2:59055542-59055561	MS.gene40753:CDS	70.0%
	CTGCAGCCTCCGCCTGAGGG+TGG	+	chr1.2:59055546-59055565	MS.gene40753:CDS	75.0%
	GGCGGAGGCTGCAGTGACGG+AGG	-	chr1.2:59055540-59055559	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	59055024	59055602	59055024	ID=MS.gene40753
chr1.2	mRNA	59055024	59055602	59055024	ID=MS.gene40753.t1;Parent=MS.gene40753
chr1.2	exon	59055024	59055218	59055024	ID=MS.gene40753.t1.exon1;Parent=MS.gene40753.t1
chr1.2	CDS	59055024	59055218	59055024	ID=cds.MS.gene40753.t1;Parent=MS.gene40753.t1
chr1.2	exon	59055308	59055389	59055308	ID=MS.gene40753.t1.exon2;Parent=MS.gene40753.t1
chr1.2	CDS	59055308	59055389	59055308	ID=cds.MS.gene40753.t1;Parent=MS.gene40753.t1
chr1.2	exon	59055493	59055602	59055493	ID=MS.gene40753.t1.exon3;Parent=MS.gene40753.t1
chr1.2	CDS	59055493	59055602	59055493	ID=cds.MS.gene40753.t1;Parent=MS.gene40753.t1

Gene Sequence

>MS.gene40753

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGTACTATCAATTTATATTTAAATTTGCATCCTCATTAATTATCATTTCTTAGTGCTCTATAAGTCGATTAACTCTCTCTGTATATTTAGGGATTTTGTCGTTATGGCAATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAGCAACCAATAACAAGAATGCCAGGTGAGCTACCCCGATAGTTGTTCATTTACTGATTTATAGTTTCTTTCTTTATGGTGTTGAATTCATGATTGATATTAATGTTATGTACTATGCATATATGTAGGGAATATAGTAGGAGATACCATGGGAGTGTCGTTCGGCAATTTGCCTCCGTCACTGCAGCCTCCGCCTGAGGGTGGATACTCCAACCTCCCATTTGTCGACTGGGGATAG

Protein sequence

>MS.gene40753.t1

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGNSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFVDWG