AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40761

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40761.t1 MTR_4g102720 50.296 169 50 2 30 197 3 138 1.38e-49 171
MS.gene40761.t1 MTR_5g039760 40.394 203 94 4 28 227 4 182 9.09e-44 154
MS.gene40761.t1 MTR_6g051425 33.091 275 172 5 33 296 13 286 1.28e-38 145
MS.gene40761.t1 MTR_0558s0020 33.708 267 148 8 30 272 35 296 1.27e-37 140
MS.gene40761.t1 MTR_7g074790 42.458 179 83 3 29 205 5 165 1.55e-36 135
MS.gene40761.t1 MTR_7g061980 50.442 113 54 2 31 141 7 119 1.29e-30 116
MS.gene40761.t1 MTR_0402s0040 38.674 181 104 5 34 209 26 204 8.96e-30 122
MS.gene40761.t1 MTR_8g446020 37.714 175 105 2 101 272 83 256 2.16e-29 117
MS.gene40761.t1 MTR_6g090505 31.418 261 156 5 67 306 10 268 4.80e-29 118
MS.gene40761.t1 MTR_1g083640 30.672 238 136 8 30 243 50 282 2.85e-24 103
MS.gene40761.t1 MTR_1g070355 28.632 234 160 4 40 268 80 311 2.64e-23 104
MS.gene40761.t1 MTR_5g015480 42.424 132 72 3 34 161 56 187 1.82e-20 91.7
MS.gene40761.t1 MTR_7g446510 32.927 164 110 0 117 280 166 329 6.98e-20 91.3
MS.gene40761.t1 MTR_1g086270 31.858 113 65 1 326 438 1 101 3.79e-18 80.5
MS.gene40761.t1 MTR_7g446480 32.317 164 111 0 117 280 151 314 7.36e-18 85.1
MS.gene40761.t1 MTR_7g446520 29.018 224 144 3 40 262 94 303 3.79e-17 83.2
MS.gene40761.t1 MTR_1g008290 33.094 139 90 2 90 228 9 144 7.26e-17 78.2
MS.gene40761.t1 MTR_4g035820 25.627 359 236 11 27 368 68 412 1.25e-15 80.5
MS.gene40761.t1 MTR_0337s0040 32.394 142 92 2 101 239 84 224 4.79e-15 75.1
MS.gene40761.t1 MTR_2g099430 42.529 87 48 1 57 141 26 112 9.16e-15 71.2
MS.gene40761.t1 MTR_2g070590 37.864 103 60 3 34 132 17 119 1.21e-14 70.9
MS.gene40761.t1 MTR_6g022070 23.810 168 123 1 326 493 1 163 1.67e-14 75.5
MS.gene40761.t1 MTR_1g013060 28.505 214 142 5 29 233 19 230 1.71e-14 73.6
MS.gene40761.t1 MTR_6g017200 41.772 79 44 2 34 110 58 136 6.30e-14 70.1
MS.gene40761.t1 MTR_2g014100 28.502 207 121 6 30 213 43 245 2.10e-13 70.9
MS.gene40761.t1 MTR_7g113010 37.600 125 64 2 34 156 457 569 4.99e-13 72.0
MS.gene40761.t1 MTR_1g057920 41.772 79 44 2 34 110 34 112 2.37e-12 64.3
MS.gene40761.t1 MTR_5g076070 22.907 227 166 5 40 260 59 282 1.73e-11 65.5
MS.gene40761.t1 MTR_1g019850 31.818 132 83 2 34 163 70 196 8.77e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40761.t1 AT4G38180 34.167 480 308 6 31 504 70 547 1.00e-90 295
MS.gene40761.t1 AT4G15090 29.897 485 334 5 31 511 48 530 1.15e-78 264
MS.gene40761.t1 AT4G15090 29.897 485 334 5 31 511 48 530 1.15e-78 264
MS.gene40761.t1 AT4G15090 29.897 485 334 5 31 511 48 530 1.15e-78 264
MS.gene40761.t1 AT4G15090 29.897 485 334 5 31 511 48 530 1.15e-78 264
MS.gene40761.t1 AT4G15090 29.897 485 334 5 31 511 48 530 1.15e-78 264
MS.gene40761.t1 AT3G22170 29.198 524 327 8 25 531 63 559 2.52e-67 233
MS.gene40761.t1 AT3G22170 29.198 524 327 8 25 531 63 559 2.52e-67 233
MS.gene40761.t1 AT1G76320 29.916 478 320 7 38 511 1 467 8.19e-64 221
MS.gene40761.t1 AT1G76320 29.916 478 320 7 38 511 1 467 2.79e-63 221
MS.gene40761.t1 AT1G76320 29.916 478 320 7 38 511 1 467 2.79e-63 221
MS.gene40761.t1 AT1G76320 29.916 478 320 7 38 511 1 467 3.09e-63 220
MS.gene40761.t1 AT5G18960 28.631 482 284 12 33 504 210 641 2.65e-58 207
MS.gene40761.t1 AT5G18960 28.631 482 284 12 33 504 210 641 2.65e-58 207
MS.gene40761.t1 AT1G52520 28.600 493 313 12 34 512 85 552 2.54e-56 201
MS.gene40761.t1 AT3G06250 29.375 480 280 12 33 504 189 617 4.63e-56 201
MS.gene40761.t1 AT3G06250 29.375 480 280 12 33 504 189 617 4.63e-56 201
MS.gene40761.t1 AT3G06250 29.375 480 280 12 33 504 189 617 4.63e-56 201
MS.gene40761.t1 AT2G27110 28.632 475 306 9 33 504 48 492 6.41e-55 199
MS.gene40761.t1 AT2G27110 28.632 475 306 9 33 504 48 492 6.41e-55 199
MS.gene40761.t1 AT1G80010 28.350 515 342 14 33 531 94 597 6.70e-54 194
MS.gene40761.t1 AT2G27110 32.776 299 198 2 206 504 52 347 5.03e-51 186
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 27 481 4.11e-48 179
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 41 495 5.62e-48 178
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 27 481 5.77e-48 178
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 27 481 5.77e-48 178
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 27 481 9.41e-48 178
MS.gene40761.t1 AT4G19990 27.236 492 304 8 40 514 27 481 9.41e-48 178
MS.gene40761.t1 AT1G10240 27.727 440 300 9 34 460 48 482 1.12e-46 174
MS.gene40761.t1 AT1G10240 27.727 440 300 9 34 460 48 482 1.12e-46 174
MS.gene40761.t1 AT2G32250 25.941 478 305 8 38 511 1 433 6.07e-46 172
MS.gene40761.t1 AT2G32250 25.941 478 305 9 38 511 42 474 7.37e-46 172
MS.gene40761.t1 AT2G32250 25.941 478 305 8 38 511 1 433 7.69e-46 172
MS.gene40761.t1 AT2G32250 25.941 478 305 8 38 511 42 474 1.05e-45 172
MS.gene40761.t1 AT2G32250 25.941 478 305 8 38 511 42 474 1.05e-45 172
MS.gene40761.t1 AT2G32250 25.941 478 305 8 38 511 42 474 1.05e-45 172
MS.gene40761.t1 AT2G32250 25.941 478 305 9 38 511 42 474 1.21e-45 172
MS.gene40761.t1 AT2G32250 25.941 478 305 9 38 511 42 474 1.21e-45 172
MS.gene40761.t1 AT4G19990 27.193 456 280 7 74 514 6 424 1.33e-44 168
MS.gene40761.t1 AT4G38170 27.329 322 221 4 204 524 16 325 9.78e-44 163
MS.gene40761.t1 AT5G28530 25.290 431 294 12 32 440 53 477 2.15e-35 141
MS.gene40761.t1 AT5G28530 25.290 431 294 12 32 440 53 477 2.15e-35 141
MS.gene40761.t1 AT5G28530 25.290 431 294 12 32 440 53 477 2.40e-35 141
MS.gene40761.t1 AT5G28530 28.571 196 129 7 32 219 53 245 3.05e-12 69.7
MS.gene40761.t1 AT5G28530 28.571 196 129 7 32 219 53 245 3.28e-12 69.7

Find 93 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAACACCATACTTTATA+AGG 0.212221 1.2:+59162918 None:intergenic
ATGATTGATTACATTTATTT+TGG 0.214048 1.2:-59163346 MS.gene40761:CDS
TTGAAGATGCTTGGGAGTTC+TGG 0.234863 1.2:-59164035 MS.gene40761:CDS
GACAACATATGAGTTGTTTC+TGG 0.253501 1.2:+59163679 None:intergenic
TATTGCCAAGAAAAGGAATA+TGG 0.275021 1.2:-59162506 MS.gene40761:CDS
CCGAAAACCATGTCTACTTT+TGG 0.282647 1.2:+59163982 None:intergenic
CCGAAAACCATGTCTACTTT+TGG 0.282647 1.2:+59164063 None:intergenic
CCTTGCTTCACAATTTGTTC+TGG 0.300300 1.2:+59163777 None:intergenic
ATCAGATCACCAATGTGTTC+TGG 0.313904 1.2:-59163384 MS.gene40761:CDS
ATGAAGAAGACTTTGACATT+AGG 0.337357 1.2:-59162839 MS.gene40761:CDS
TTGAAGATGCTTGGGAGTTA+TGG 0.347667 1.2:-59163954 MS.gene40761:CDS
TGTACAATATAAAGGAGAAA+TGG 0.347671 1.2:-59162874 MS.gene40761:CDS
ACATCCCTCCTTATAAAGTA+TGG 0.358569 1.2:-59162926 MS.gene40761:CDS
TGAAGAAGACTTTGACATTA+GGG 0.366463 1.2:-59162838 MS.gene40761:CDS
ATGGCGAGTGACCTTCGATT+TGG 0.374680 1.2:-59162479 MS.gene40761:CDS
GCAAAGAATGTACAATATAA+AGG 0.392434 1.2:-59162882 MS.gene40761:CDS
TCGGTAGCTTTGAAGATGCT+TGG 0.398691 1.2:-59163963 MS.gene40761:CDS
TCGGTAGCTTTGAAGATGCT+TGG 0.398691 1.2:-59164044 MS.gene40761:CDS
ACGTGGCATTTGATGCAAAA+TGG 0.399037 1.2:-59163055 MS.gene40761:CDS
CGGTAGCTTTGAAGATGCTT+GGG 0.400248 1.2:-59163962 MS.gene40761:CDS
CGGTAGCTTTGAAGATGCTT+GGG 0.400248 1.2:-59164043 MS.gene40761:CDS
GATACAAGATGAATGAAGAT+TGG 0.401677 1.2:-59164089 None:intergenic
CATAAACCATGGTAAGCTTC+AGG 0.412043 1.2:+59163079 None:intergenic
GACTTTAAGAAATGCATGTA+TGG 0.419899 1.2:-59162983 MS.gene40761:CDS
AGTAGGAGGCAGAACTAACT+TGG 0.425960 1.2:-59163557 MS.gene40761:CDS
TCAGATCACCAATGTGTTCT+GGG 0.429444 1.2:-59163383 MS.gene40761:CDS
GTACAATATAAAGGAGAAAT+GGG 0.433816 1.2:-59162873 MS.gene40761:CDS
AGGTTTGTTTGTTGCAAAGA+AGG 0.437860 1.2:-59163853 MS.gene40761:CDS
TGGAATAAGTCGAAAATAGT+AGG 0.440115 1.2:+59162599 None:intergenic
TACGGTGGTAAGGTTGGATT+CGG 0.443498 1.2:-59163925 MS.gene40761:CDS
TACTGATCAAGCCAAATCAA+TGG 0.443726 1.2:-59163125 MS.gene40761:CDS
TGGGAGTTCTGGACAACTTA+CGG 0.445098 1.2:-59164024 MS.gene40761:CDS
AAGAACAATTTGAAGATGGT+TGG 0.449713 1.2:-59162949 MS.gene40761:CDS
GAGAAAGAAGTTTGGTACAA+GGG 0.449883 1.2:-59163492 MS.gene40761:CDS
GATTTACTGAGTAAAGAAGT+AGG 0.459354 1.2:-59163574 MS.gene40761:CDS
AAGATGTTGTAGAAGAAAAG+TGG 0.460842 1.2:-59162721 MS.gene40761:CDS
AGGATGGTTCGGTTTCTTGT+AGG 0.462507 1.2:-59163873 MS.gene40761:CDS
TTTGTTCTGGTCTCTAGTCT+AGG 0.464956 1.2:+59163790 None:intergenic
CTTACGGTGTGAATGAAGAT+TGG 0.472798 1.2:-59164008 MS.gene40761:CDS
TTGATCAAAAGAATTATCTT+CGG 0.474061 1.2:-59163522 MS.gene40761:CDS
ACAAATTGTGAAGCAAGGTT+AGG 0.474466 1.2:-59163772 MS.gene40761:CDS
ATCTACTTATTGCCAAGAAA+AGG 0.476346 1.2:-59162513 MS.gene40761:CDS
AATAGTAGGATTATAAAGCT+TGG 0.484405 1.2:+59162613 None:intergenic
ACACTTAGGAAATTTAATGA+AGG 0.489904 1.2:-59163026 MS.gene40761:CDS
GAATGTGGATGGAAAATTGA+TGG 0.494783 1.2:-59163743 MS.gene40761:CDS
GATGAAGAACAATTTGAAGA+TGG 0.510431 1.2:-59162953 MS.gene40761:CDS
CACTTAGGAAATTTAATGAA+GGG 0.513608 1.2:-59163025 MS.gene40761:CDS
TGGGAGTTATGGACAACTTA+CGG 0.518966 1.2:-59163943 MS.gene40761:CDS
ATTGCCAAGAAAAGGAATAT+GGG 0.520097 1.2:-59162505 MS.gene40761:CDS
AGAGAAAGAAGTTTGGTACA+AGG 0.525956 1.2:-59163493 MS.gene40761:CDS
GTAAGGTTGGATTCGGAGTG+AGG 0.527766 1.2:-59163918 MS.gene40761:CDS
ATGGACAACTTACGGTGGTA+AGG 0.530401 1.2:-59163935 MS.gene40761:CDS
TATGTTGTCTTCTCAACGAA+AGG 0.532534 1.2:-59163665 MS.gene40761:CDS
AAAGTATTGCAGTAAATAGC+CGG 0.537802 1.2:+59163465 None:intergenic
TGATAAGCATGGTAGAAAGT+TGG 0.539495 1.2:+59163421 None:intergenic
CAAAATGGAATTAAACACTT+AGG 0.542707 1.2:-59163040 MS.gene40761:CDS
TTAGGACTTAAGAATGTGGA+TGG 0.548535 1.2:-59163754 MS.gene40761:CDS
ACAACTTACGGTGGTAAGGT+TGG 0.552623 1.2:-59163931 MS.gene40761:CDS
AGAAAAGGAATATGGGAGAA+TGG 0.553822 1.2:-59162498 MS.gene40761:CDS
GCAAAAGTAACTCGTGGTTG+TGG 0.561945 1.2:+59163701 None:intergenic
AGTTTGGTACAAGGGAAGGC+CGG 0.568469 1.2:-59163484 MS.gene40761:CDS
CAGACCACTAGCAATCATCT+CGG 0.572687 1.2:-59163278 MS.gene40761:CDS
GAACACCATACTTTATAAGG+AGG 0.576736 1.2:+59162921 None:intergenic
ATGTGTTCTGGGCAGATGCT+AGG 0.582023 1.2:-59163372 MS.gene40761:CDS
AAGAAGTTTGGTACAAGGGA+AGG 0.587974 1.2:-59163488 MS.gene40761:CDS
CTTAGGAAATTTAATGAAGG+GGG 0.589615 1.2:-59163023 MS.gene40761:CDS
ACTTAGGAAATTTAATGAAG+GGG 0.591209 1.2:-59163024 MS.gene40761:CDS
CTTACCATGGTTTATGTACG+TGG 0.594189 1.2:-59163072 MS.gene40761:CDS
AGACCACTAGCAATCATCTC+GGG 0.603216 1.2:-59163277 MS.gene40761:CDS
AACACCATACTTTATAAGGA+GGG 0.603443 1.2:+59162922 None:intergenic
AAATTATGGCTCCTCGATGG+TGG 0.609394 1.2:+59163248 None:intergenic
CCAGAACAAATTGTGAAGCA+AGG 0.610911 1.2:-59163777 MS.gene40761:CDS
TTGGAAGCCAAAAGTAGACA+TGG 0.615146 1.2:-59163989 MS.gene40761:CDS
TTGGAAGCCAAAAGTAGACA+TGG 0.615146 1.2:-59164070 MS.gene40761:CDS
GCAATACTTTCAAAGAAAGA+CGG 0.623662 1.2:-59163452 MS.gene40761:CDS
GTAGGAGGCAGAACTAACTT+GGG 0.636519 1.2:-59163556 MS.gene40761:CDS
TTTCTGGCAAAAGTAACTCG+TGG 0.639142 1.2:+59163695 None:intergenic
GTTATGCCTGAAGCTTACCA+TGG 0.640428 1.2:-59163085 MS.gene40761:CDS
TAATATCTAGCTGATAAGCA+TGG 0.650741 1.2:+59163410 None:intergenic
AGCATCTGCCCAGAACACAT+TGG 0.654768 1.2:+59163375 None:intergenic
ATAGTGTCGGTGTACAACAA+AGG 0.658150 1.2:-59163606 MS.gene40761:CDS
AATGCCACGTACATAAACCA+TGG 0.659165 1.2:+59163068 None:intergenic
CAAACAACTCATACTCACGT+TGG 0.665525 1.2:+59162579 None:intergenic
GTAAGCTTCAGGCATAACCT+CGG 0.674454 1.2:+59163090 None:intergenic
AATCCCGAGATGATTGCTAG+TGG 0.674621 1.2:+59163274 None:intergenic
ATTGAACTAGCTGATAGTGT+CGG 0.677446 1.2:-59163619 MS.gene40761:CDS
AGCATGCTTTGTGAAAAGCA+TGG 0.679180 1.2:-59162555 MS.gene40761:CDS
AAGGTTAGGACTTAAGAATG+TGG 0.679784 1.2:-59163758 MS.gene40761:CDS
AATGGCAAAAGCATTAGCCG+AGG 0.699479 1.2:-59163107 MS.gene40761:CDS
TTACTGAGTAAAGAAGTAGG+AGG 0.709735 1.2:-59163571 MS.gene40761:CDS
AAACTGCTGAGTCATACGAG+TGG 0.746891 1.2:-59163201 MS.gene40761:CDS
TCGGGATTTAACCACCATCG+AGG 0.749121 1.2:-59163259 MS.gene40761:CDS
GAGTTATGGACAACTTACGG+TGG 0.777362 1.2:-59163940 MS.gene40761:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAAAAATTTTATGAGTA+AGG - chr1.2:59163752-59163771 MS.gene40761:CDS 10.0%
!!! ATGATTGATTACATTTATTT+TGG - chr1.2:59163183-59163202 MS.gene40761:CDS 15.0%
!! TTGATCAAAAGAATTATCTT+CGG - chr1.2:59163007-59163026 MS.gene40761:CDS 20.0%
! AATAGTAGGATTATAAAGCT+TGG + chr1.2:59163919-59163938 None:intergenic 25.0%
! ACACTTAGGAAATTTAATGA+AGG - chr1.2:59163503-59163522 MS.gene40761:CDS 25.0%
! ACTTAGGAAATTTAATGAAG+GGG - chr1.2:59163505-59163524 MS.gene40761:CDS 25.0%
! AGATTATACACACAGAAAAA+AGG - chr1.2:59162636-59162655 MS.gene40761:CDS 25.0%
! CAAAATGGAATTAAACACTT+AGG - chr1.2:59163489-59163508 MS.gene40761:CDS 25.0%
! CACTTAGGAAATTTAATGAA+GGG - chr1.2:59163504-59163523 MS.gene40761:CDS 25.0%
! GAAAAAAAGAGAAAGAAGTT+TGG - chr1.2:59163029-59163048 MS.gene40761:CDS 25.0%
! GCAAAGAATGTACAATATAA+AGG - chr1.2:59163647-59163666 MS.gene40761:CDS 25.0%
! GTACAATATAAAGGAGAAAT+GGG - chr1.2:59163656-59163675 MS.gene40761:CDS 25.0%
! TGTACAATATAAAGGAGAAA+TGG - chr1.2:59163655-59163674 MS.gene40761:CDS 25.0%
!! AAATTTTATGAGTAAGGACT+TGG - chr1.2:59163758-59163777 MS.gene40761:CDS 25.0%
!!! TCTTCAGTCTTGAAATTTTT+TGG + chr1.2:59163866-59163885 None:intergenic 25.0%
AACACCATACTTTATAAGGA+GGG + chr1.2:59163610-59163629 None:intergenic 30.0%
AAGAACAATTTGAAGATGGT+TGG - chr1.2:59163580-59163599 MS.gene40761:CDS 30.0%
AAGATGTTGTAGAAGAAAAG+TGG - chr1.2:59163808-59163827 MS.gene40761:CDS 30.0%
ATCTACTTATTGCCAAGAAA+AGG - chr1.2:59164016-59164035 MS.gene40761:CDS 30.0%
ATGAAGAAGACTTTGACATT+AGG - chr1.2:59163690-59163709 MS.gene40761:CDS 30.0%
ATGGTGTTCAGAAAAATGAT+TGG - chr1.2:59163622-59163641 MS.gene40761:CDS 30.0%
ATTGCCAAGAAAAGGAATAT+GGG - chr1.2:59164024-59164043 MS.gene40761:CDS 30.0%
CTTAGGAAATTTAATGAAGG+GGG - chr1.2:59163506-59163525 MS.gene40761:CDS 30.0%
GATGAAGAACAATTTGAAGA+TGG - chr1.2:59163576-59163595 MS.gene40761:CDS 30.0%
GATTTACTGAGTAAAGAAGT+AGG - chr1.2:59162955-59162974 MS.gene40761:CDS 30.0%
GCAATACTTTCAAAGAAAGA+CGG - chr1.2:59163077-59163096 MS.gene40761:CDS 30.0%
TAATATCTAGCTGATAAGCA+TGG + chr1.2:59163122-59163141 None:intergenic 30.0%
TAATGCAGCTCCAAAAATTA+TGG + chr1.2:59163298-59163317 None:intergenic 30.0%
TATACACACAGAAAAAAGGA+TGG - chr1.2:59162640-59162659 MS.gene40761:CDS 30.0%
TATTGCCAAGAAAAGGAATA+TGG - chr1.2:59164023-59164042 MS.gene40761:CDS 30.0%
TCTGAACACCATACTTTATA+AGG + chr1.2:59163614-59163633 None:intergenic 30.0%
TGAAGAAGACTTTGACATTA+GGG - chr1.2:59163691-59163710 MS.gene40761:CDS 30.0%
TGATCAGTAAAAACTGTTTG+AGG + chr1.2:59163393-59163412 None:intergenic 30.0%
TGGAATAAGTCGAAAATAGT+AGG + chr1.2:59163933-59163952 None:intergenic 30.0%
! GACTTTAAGAAATGCATGTA+TGG - chr1.2:59163546-59163565 MS.gene40761:CDS 30.0%
! TGTATTCTTTTCCAAATCGA+AGG + chr1.2:59164064-59164083 None:intergenic 30.0%
!! AAAGTATTGCAGTAAATAGC+CGG + chr1.2:59163067-59163086 None:intergenic 30.0%
!! TAATGCTTTTGCCATTGATT+TGG + chr1.2:59163418-59163437 None:intergenic 30.0%
AAGGTTAGGACTTAAGAATG+TGG - chr1.2:59162771-59162790 MS.gene40761:CDS 35.0%
ACAAATTGTGAAGCAAGGTT+AGG - chr1.2:59162757-59162776 MS.gene40761:CDS 35.0%
ACACAGAAAAAAGGATGGTT+CGG - chr1.2:59162645-59162664 MS.gene40761:CDS 35.0%
ACATCCCTCCTTATAAAGTA+TGG - chr1.2:59163603-59163622 MS.gene40761:CDS 35.0%
AGAAAAGGAATATGGGAGAA+TGG - chr1.2:59164031-59164050 MS.gene40761:CDS 35.0%
AGAGAAAGAAGTTTGGTACA+AGG - chr1.2:59163036-59163055 MS.gene40761:CDS 35.0%
GAACACCATACTTTATAAGG+AGG + chr1.2:59163611-59163630 None:intergenic 35.0%
GAATGTGGATGGAAAATTGA+TGG - chr1.2:59162786-59162805 MS.gene40761:CDS 35.0%
GAGAAAGAAGTTTGGTACAA+GGG - chr1.2:59163037-59163056 MS.gene40761:CDS 35.0%
TACTGATCAAGCCAAATCAA+TGG - chr1.2:59163404-59163423 MS.gene40761:CDS 35.0%
TATGTTGTCTTCTCAACGAA+AGG - chr1.2:59162864-59162883 MS.gene40761:CDS 35.0%
TGATAAGCATGGTAGAAAGT+TGG + chr1.2:59163111-59163130 None:intergenic 35.0%
TTACTGAGTAAAGAAGTAGG+AGG - chr1.2:59162958-59162977 MS.gene40761:CDS 35.0%
TTAGGACTTAAGAATGTGGA+TGG - chr1.2:59162775-59162794 MS.gene40761:CDS 35.0%
! AGGTTTGTTTGTTGCAAAGA+AGG - chr1.2:59162676-59162695 MS.gene40761:CDS 35.0%
! ATTGAACTAGCTGATAGTGT+CGG - chr1.2:59162910-59162929 MS.gene40761:CDS 35.0%
! CCAAAAGTAGACATGGTTTT+CGG - chr1.2:59162466-59162485 MS.gene40761:CDS 35.0%
! GACAACATATGAGTTGTTTC+TGG + chr1.2:59162853-59162872 None:intergenic 35.0%
! TTCTCCCATATTCCTTTTCT+TGG + chr1.2:59164031-59164050 None:intergenic 35.0%
!!! ATTGATGGTGCATGATTTTG+TGG - chr1.2:59162801-59162820 MS.gene40761:CDS 35.0%
AAGAAGTTTGGTACAAGGGA+AGG - chr1.2:59163041-59163060 MS.gene40761:CDS 40.0%
AATGCCACGTACATAAACCA+TGG + chr1.2:59163464-59163483 None:intergenic 40.0%
ACGTGGCATTTGATGCAAAA+TGG - chr1.2:59163474-59163493 MS.gene40761:CDS 40.0%
AGCATGCTTTGTGAAAAGCA+TGG - chr1.2:59163974-59163993 MS.gene40761:CDS 40.0%
ATCAGATCACCAATGTGTTC+TGG - chr1.2:59163145-59163164 MS.gene40761:CDS 40.0%
CAAAAATTATGGCTCCTCGA+TGG + chr1.2:59163287-59163306 None:intergenic 40.0%
CAAACAACTCATACTCACGT+TGG + chr1.2:59163953-59163972 None:intergenic 40.0%
CATAAACCATGGTAAGCTTC+AGG + chr1.2:59163453-59163472 None:intergenic 40.0%
CCAGAACAAATTGTGAAGCA+AGG - chr1.2:59162752-59162771 MS.gene40761:CDS 40.0%
CTTACCATGGTTTATGTACG+TGG - chr1.2:59163457-59163476 MS.gene40761:CDS 40.0%
CTTACGGTGTGAATGAAGAT+TGG - chr1.2:59162521-59162540 MS.gene40761:CDS 40.0%
TCAGATCACCAATGTGTTCT+GGG - chr1.2:59163146-59163165 MS.gene40761:CDS 40.0%
TGGGAGTTATGGACAACTTA+CGG - chr1.2:59162586-59162605 MS.gene40761:CDS 40.0%
TTGAAGATGCTTGGGAGTTA+TGG - chr1.2:59162575-59162594 MS.gene40761:CDS 40.0%
TTGGAAGCCAAAAGTAGACA+TGG - chr1.2:59162459-59162478 MS.gene40761:CDS 40.0%
TTTGTTCTGGTCTCTAGTCT+AGG + chr1.2:59162742-59162761 None:intergenic 40.0%
! CATCGAGGAGCCATAATTTT+TGG - chr1.2:59163285-59163304 MS.gene40761:CDS 40.0%
! CCGAAAACCATGTCTACTTT+TGG + chr1.2:59162469-59162488 None:intergenic 40.0%
! CCTTGCTTCACAATTTGTTC+TGG + chr1.2:59162755-59162774 None:intergenic 40.0%
! GTTTTATGATCTCGCTTGTC+TGG + chr1.2:59162712-59162731 None:intergenic 40.0%
! TTTCTGGCAAAAGTAACTCG+TGG + chr1.2:59162837-59162856 None:intergenic 40.0%
!! ATAGTGTCGGTGTACAACAA+AGG - chr1.2:59162923-59162942 MS.gene40761:CDS 40.0%
AAACTGCTGAGTCATACGAG+TGG - chr1.2:59163328-59163347 MS.gene40761:CDS 45.0%
AAATTATGGCTCCTCGATGG+TGG + chr1.2:59163284-59163303 None:intergenic 45.0%
AATCCCGAGATGATTGCTAG+TGG + chr1.2:59163258-59163277 None:intergenic 45.0%
ACAACTTACGGTGGTAAGGT+TGG - chr1.2:59162598-59162617 MS.gene40761:CDS 45.0%
AGACCACTAGCAATCATCTC+GGG - chr1.2:59163252-59163271 MS.gene40761:CDS 45.0%
AGTAGGAGGCAGAACTAACT+TGG - chr1.2:59162972-59162991 MS.gene40761:CDS 45.0%
ATGGACAACTTACGGTGGTA+AGG - chr1.2:59162594-59162613 MS.gene40761:CDS 45.0%
CAGACCACTAGCAATCATCT+CGG - chr1.2:59163251-59163270 MS.gene40761:CDS 45.0%
GAGTTATGGACAACTTACGG+TGG - chr1.2:59162589-59162608 MS.gene40761:CDS 45.0%
GCAAAAGTAACTCGTGGTTG+TGG + chr1.2:59162831-59162850 None:intergenic 45.0%
GTAAGCTTCAGGCATAACCT+CGG + chr1.2:59163442-59163461 None:intergenic 45.0%
GTAGGAGGCAGAACTAACTT+GGG - chr1.2:59162973-59162992 MS.gene40761:CDS 45.0%
GTTATGCCTGAAGCTTACCA+TGG - chr1.2:59163444-59163463 MS.gene40761:CDS 45.0%
TACGGTGGTAAGGTTGGATT+CGG - chr1.2:59162604-59162623 MS.gene40761:CDS 45.0%
TTGAAGATGCTTGGGAGTTC+TGG - chr1.2:59162494-59162513 MS.gene40761:CDS 45.0%
! GATGGTGCATGATTTTGTGG+AGG - chr1.2:59162804-59162823 MS.gene40761:CDS 45.0%
!! AATGGCAAAAGCATTAGCCG+AGG - chr1.2:59163422-59163441 MS.gene40761:CDS 45.0%
!! AGGATGGTTCGGTTTCTTGT+AGG - chr1.2:59162656-59162675 MS.gene40761:CDS 45.0%
!! CGGTAGCTTTGAAGATGCTT+GGG - chr1.2:59162486-59162505 MS.gene40761:CDS 45.0%
!! TCGGTAGCTTTGAAGATGCT+TGG - chr1.2:59162485-59162504 MS.gene40761:CDS 45.0%
!! TGGGAGTTCTGGACAACTTA+CGG - chr1.2:59162505-59162524 MS.gene40761:CDS 45.0%
AGCATCTGCCCAGAACACAT+TGG + chr1.2:59163157-59163176 None:intergenic 50.0%
AGTTTGGTACAAGGGAAGGC+CGG - chr1.2:59163045-59163064 MS.gene40761:CDS 50.0%
ATGGCGAGTGACCTTCGATT+TGG - chr1.2:59164050-59164069 MS.gene40761:CDS 50.0%
GTAAGGTTGGATTCGGAGTG+AGG - chr1.2:59162611-59162630 MS.gene40761:CDS 50.0%
TCGGGATTTAACCACCATCG+AGG - chr1.2:59163270-59163289 MS.gene40761:CDS 50.0%
! ATGTGTTCTGGGCAGATGCT+AGG - chr1.2:59163157-59163176 MS.gene40761:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 59162448 59164103 59162448 ID=MS.gene40761
chr1.2 mRNA 59162448 59164103 59162448 ID=MS.gene40761.t1;Parent=MS.gene40761
chr1.2 exon 59162448 59164103 59162448 ID=MS.gene40761.t1.exon1;Parent=MS.gene40761.t1
chr1.2 CDS 59162448 59164103 59162448 ID=cds.MS.gene40761.t1;Parent=MS.gene40761.t1
Gene Sequence

>MS.gene40761

ATGAATGAAGATTGGAAGCCAAAAGTAGACATGGTTTTCGGTAGCTTTGAAGATGCTTGGGAGTTCTGGACAACTTACGGTGTGAATGAAGATTGGAAGCCAAAAGTAGACATGGTTTTCGGTAGCTTTGAAGATGCTTGGGAGTTATGGACAACTTACGGTGGTAAGGTTGGATTCGGAGTGAGGAAAGATTATACACACAGAAAAAAGGATGGTTCGGTTTCTTGTAGGTTTGTTTGTTGCAAAGAAGGCATACGAAAGCCAGACAAGCGAGATCATAAAACAAAGAATCCTAGACTAGAGACCAGAACAAATTGTGAAGCAAGGTTAGGACTTAAGAATGTGGATGGAAAATTGATGGTGCATGATTTTGTGGAGGACCACAACCACGAGTTACTTTTGCCAGAAACAACTCATATGTTGTCTTCTCAACGAAAGGTTTCTGAAACTCATTGTCAACAAATTGAACTAGCTGATAGTGTCGGTGTACAACAAAGGAAATCGTTTGATTTACTGAGTAAAGAAGTAGGAGGCAGAACTAACTTGGGTTTTATTCGTCTTGATCAAAAGAATTATCTTCGGAAAAAAAGAGAAAGAAGTTTGGTACAAGGGAAGGCCGGCTATTTACTGCAATACTTTCAAAGAAAGACGGTAGAAAATCCAACTTTCTACCATGCTTATCAGCTAGATATTAAAGATCAGATCACCAATGTGTTCTGGGCAGATGCTAGGATGATGATTGATTACATTTATTTTGGTGATGTTGTTTCTCTTGATTCAACATATTGCACAAATAGTTCGCACAGACCACTAGCAATCATCTCGGGATTTAACCACCATCGAGGAGCCATAATTTTTGGAGCTGCATTATTATACGACGAAACTGCTGAGTCATACGAGTGGCTCTTTGAGACATTTCTAGAAGCACATAAGCAGAAAATGCCTCAAACAGTTTTTACTGATCAAGCCAAATCAATGGCAAAAGCATTAGCCGAGGTTATGCCTGAAGCTTACCATGGTTTATGTACGTGGCATTTGATGCAAAATGGAATTAAACACTTAGGAAATTTAATGAAGGGGGAATCTTATTTTCTTACTGACTTTAAGAAATGCATGTATGGTTATGAAGATGAAGAACAATTTGAAGATGGTTGGACATCCCTCCTTATAAAGTATGGTGTTCAGAAAAATGATTGGTTGCAAAGAATGTACAATATAAAGGAGAAATGGGCATCTTGTTACATGAAGAAGACTTTGACATTAGGGATGCGAAACACTCAACTTAGTGAAAGTGTGAATGCAGATATTAAAAATTTTATGAGTAAGGACTTGGATTTAATTAAATTTTGTGAGCGTTTTGAAGATGTTGTAGAAGAAAAGTGGTATAAAGAATTGAAGTGTGAATTTGAAGCACGCCAAAAAATTTCAAGACTGAAGAACTCATATTCTGATATTTTGCAACAAGTGTCCAAGCTTTATAATCCTACTATTTTCGACTTATTCCAACGTGAGTATGAGTTGTTTGAAGCATGCTTTGTGAAAAGCATGGACATACAAACATCATTATCATCTACTTATTGCCAAGAAAAGGAATATGGGAGAATGGCGAGTGACCTTCGATTTGGAAAAGAATACAATTTCTTGCTCTTGTCGTAA
Protein sequence

>MS.gene40761.t1

MNEDWKPKVDMVFGSFEDAWEFWTTYGVNEDWKPKVDMVFGSFEDAWELWTTYGGKVGFGVRKDYTHRKKDGSVSCRFVCCKEGIRKPDKRDHKTKNPRLETRTNCEARLGLKNVDGKLMVHDFVEDHNHELLLPETTHMLSSQRKVSETHCQQIELADSVGVQQRKSFDLLSKEVGGRTNLGFIRLDQKNYLRKKRERSLVQGKAGYLLQYFQRKTVENPTFYHAYQLDIKDQITNVFWADARMMIDYIYFGDVVSLDSTYCTNSSHRPLAIISGFNHHRGAIIFGAALLYDETAESYEWLFETFLEAHKQKMPQTVFTDQAKSMAKALAEVMPEAYHGLCTWHLMQNGIKHLGNLMKGESYFLTDFKKCMYGYEDEEQFEDGWTSLLIKYGVQKNDWLQRMYNIKEKWASCYMKKTLTLGMRNTQLSESVNADIKNFMSKDLDLIKFCERFEDVVEEKWYKELKCEFEARQKISRLKNSYSDILQQVSKLYNPTIFDLFQREYELFEACFVKSMDIQTSLSSTYCQEKEYGRMASDLRFGKEYNFLLLS