AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40779

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40779.t1 MTR_5g095070 61.056 303 82 8 2 304 5 271 1.25e-111 326
MS.gene40779.t1 MTR_1g084150 76.824 233 28 2 150 356 1 233 1.25e-110 322
MS.gene40779.t1 MTR_5g080030 64.935 231 73 2 37 267 44 266 4.83e-98 296
MS.gene40779.t1 MTR_5g080030 53.254 169 58 6 189 356 264 412 6.76e-38 140
MS.gene40779.t1 MTR_4g015240 51.220 82 19 2 265 345 39 100 1.07e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCGTTGGATATGTTAAA+TGG 0.182684 1.2:-59366644 MS.gene40779:CDS
CACAATTCCATTGGTGAGTT+TGG 0.209381 1.2:+59369214 None:intergenic
TACTGGTTTATCTCAATTAA+TGG 0.258551 1.2:-59367652 MS.gene40779:CDS
TCCCTTCTTCCCAGTTGATT+TGG 0.271998 1.2:-59366675 MS.gene40779:CDS
CCTTGGGCGCTGCCGGTTGC+AGG 0.289338 1.2:+59368028 None:intergenic
TGTCAAACTAACTGAAGTTT+TGG 0.307954 1.2:-59369138 MS.gene40779:CDS
AGTTGTCTCTGCACTCAAAT+TGG 0.316435 1.2:-59368140 MS.gene40779:CDS
TTCTTGCTTTCCTCCACATA+TGG 0.365771 1.2:+59366926 None:intergenic
TTTGTAAAGGTACGCGTAAA+AGG 0.367652 1.2:-59367689 MS.gene40779:intron
TTCAGTTAGTTTGACATAGA+AGG 0.372838 1.2:+59369145 None:intergenic
CAGTAAGTATCCAAATCAAC+TGG 0.378693 1.2:+59366665 None:intergenic
TTCTACAGGTTTCCTGCAAC+CGG 0.391216 1.2:-59368040 MS.gene40779:CDS
GAAGAGCACTCAAGATCTTA+AGG 0.392322 1.2:-59366825 MS.gene40779:CDS
AGGTTGGTCTTCAAAACAAA+TGG 0.395740 1.2:-59368168 MS.gene40779:CDS
TTGAGAAATTGTACTTCTAC+AGG 0.399495 1.2:-59368054 MS.gene40779:CDS
AATCCTTATAACCTGTCATT+TGG 0.408344 1.2:+59367580 None:intergenic
CAACCGCTAGTATAGTCATT+TGG 0.413628 1.2:+59366616 None:intergenic
GGAGGAAAGCAAGAAGATAA+AGG 0.418994 1.2:-59366918 MS.gene40779:CDS
AATGGAAGGCAACAAAACTA+TGG 0.422265 1.2:-59369258 MS.gene40779:CDS
GTCTCTATCTCTAAGTCATC+AGG 0.427299 1.2:+59369167 None:intergenic
TGGTGACTTCCAAGTAAAAC+AGG 0.431064 1.2:+59368455 None:intergenic
TTAGATACCTTGGGCGCTGC+CGG 0.439165 1.2:+59368021 None:intergenic
CTGGTGAAAACCTCCTCTTC+TGG 0.440306 1.2:+59368435 None:intergenic
CCCATTGGACACAATTCCAT+TGG 0.444629 1.2:+59369205 None:intergenic
ATCCAAATCAACTGGGAAGA+AGG 0.454997 1.2:+59366673 None:intergenic
GCTCAAGATCCGGACATTGA+TGG 0.455328 1.2:-59367093 MS.gene40779:CDS
GTTCGTTGGATATGTTAAAT+GGG 0.459203 1.2:-59366643 MS.gene40779:CDS
TATTTGATATCCACTTTGTC+CGG 0.459963 1.2:+59367146 None:intergenic
AAGACAAAGAAAAGAAGAAA+CGG 0.466286 1.2:-59367182 MS.gene40779:CDS
TACGCATGGCTGTTGATGCT+TGG 0.488165 1.2:-59367059 MS.gene40779:CDS
CACCCGAGGTTCTTGACAGC+CGG 0.488188 1.2:+59367245 None:intergenic
TTTGGATACTTACTGTTCGT+TGG 0.489477 1.2:-59366657 MS.gene40779:CDS
AGCCGGCTGTCAAGAACCTC+GGG 0.489585 1.2:-59367247 MS.gene40779:CDS
ACAGAAGAATAGTTTGGTAG+TGG 0.492096 1.2:+59366752 None:intergenic
CCAATGGAATTGTGTCCAAT+GGG 0.492590 1.2:-59369205 MS.gene40779:CDS
TTCTTAATTAAAGCAGGAGC+CGG 0.497515 1.2:-59367264 MS.gene40779:intron
AGGAAGCAGAACTCGACTAC+TGG 0.510036 1.2:-59367669 MS.gene40779:CDS
CCTGCAACCGGCAGCGCCCA+AGG 0.510627 1.2:-59368028 MS.gene40779:intron
ACCAATGGAATTGTGTCCAA+TGG 0.516036 1.2:-59369206 MS.gene40779:CDS
ATCGAGCACATTCATGCTTG+AGG 0.516753 1.2:+59367121 None:intergenic
CAACAATGCAGCTGTGGAGT+TGG 0.523389 1.2:-59366729 MS.gene40779:CDS
TTGTTGACAGAAGAATAGTT+TGG 0.526138 1.2:+59366746 None:intergenic
TCCAAATCAACTGGGAAGAA+GGG 0.526508 1.2:+59366674 None:intergenic
TTCTGTCAACAATGCAGCTG+TGG 0.527890 1.2:-59366735 MS.gene40779:CDS
CATTTGGCTAGAGTAGTCCT+TGG 0.531068 1.2:+59367596 None:intergenic
TTGGCATCTGAAGGTGATGA+TGG 0.536024 1.2:+59369233 None:intergenic
TACTTGGAAGTCACCAGAAG+AGG 0.540823 1.2:-59368448 MS.gene40779:CDS
GATCATTGACAATAAACAGA+TGG 0.543332 1.2:-59366876 MS.gene40779:CDS
GGTTGGTCTTCAAAACAAAT+GGG 0.543380 1.2:-59368167 MS.gene40779:CDS
TCGATTAAAAGCTCAAGATC+CGG 0.550386 1.2:-59367103 MS.gene40779:CDS
TGATATCCACTTTGTCCGGT+AGG 0.555314 1.2:+59367150 None:intergenic
GAGCCGGCTGTCAAGAACCT+CGG 0.562075 1.2:-59367248 MS.gene40779:CDS
GACTACTCTAGCCAAATGAC+AGG 0.564216 1.2:-59367591 MS.gene40779:intron
AGAAGAGTACTGTTTCCCAT+TGG 0.564784 1.2:+59369190 None:intergenic
GTCTCTGCACTCAAATTGGA+AGG 0.566571 1.2:-59368136 MS.gene40779:CDS
AAGATCTTAAGGAGGAAAAG+AGG 0.571062 1.2:-59366814 MS.gene40779:CDS
AAACGGCGAGGTCCTCCTAC+CGG 0.572639 1.2:-59367165 MS.gene40779:CDS
TTCCTAGCTCAAACAATGGA+AGG 0.574513 1.2:-59369272 None:intergenic
TAGTCGTAACCGCACACACT+AGG 0.575038 1.2:+59366791 None:intergenic
GAATGTGGCAAAGGTTTCCA+AGG 0.580859 1.2:-59367613 MS.gene40779:CDS
GGGCACTGAAGATCTTAAGA+AGG 0.581617 1.2:-59366855 MS.gene40779:CDS
ATCATTGACAATAAACAGAT+GGG 0.586725 1.2:-59366875 MS.gene40779:CDS
AGATAATCTGAATGTGGCAA+AGG 0.590122 1.2:-59367622 MS.gene40779:CDS
TTGGAAGTCACCAGAAGAGG+AGG 0.590853 1.2:-59368445 MS.gene40779:CDS
TATCCACTTTGTCCGGTAGG+AGG 0.594215 1.2:+59367153 None:intergenic
TTGGTGAGTTTGGCATCTGA+AGG 0.614422 1.2:+59369224 None:intergenic
TCAGATGCCAAACTCACCAA+TGG 0.618895 1.2:-59369221 MS.gene40779:CDS
TTTCATGTAGCCATATGTGG+AGG 0.619171 1.2:-59366936 MS.gene40779:intron
GGTCCTCCTACCGGACAAAG+TGG 0.623840 1.2:-59367156 MS.gene40779:CDS
ACAAGTCTAAGATTGTCTCT+CGG 0.631739 1.2:+59368479 None:intergenic
AAGCATCAACAGCCATGCGT+AGG 0.637247 1.2:+59367061 None:intergenic
GAAAAGAGGCCTAGTGTGTG+CGG 0.639000 1.2:-59366800 MS.gene40779:CDS
AGTAAGTATCCAAATCAACT+GGG 0.663991 1.2:+59366666 None:intergenic
GAGCACTCAAGATCTTAAGG+AGG 0.665195 1.2:-59366822 MS.gene40779:CDS
GGTGCTTGTAGAAGCACCCG+AGG 0.679117 1.2:+59367231 None:intergenic
TGATGAAGATAATCTGAATG+TGG 0.680646 1.2:-59367628 MS.gene40779:CDS
AAAGAAAAGAAGAAACGGCG+AGG 0.681943 1.2:-59367177 MS.gene40779:CDS
AGACATGAGATTGAACCCAG+AGG 0.774697 1.2:+59369112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAGATTTAATATATTGTAA+TGG - chr1.2:59367679-59367698 MS.gene40779:CDS 15.0%
!!! AAAGTTTTATGAGATAATAA+AGG - chr1.2:59367337-59367356 MS.gene40779:intron 15.0%
!!! AAGTTTTATGAGATAATAAA+GGG - chr1.2:59367338-59367357 MS.gene40779:intron 15.0%
!! AAATTAAATCATGTGATCAT+GGG + chr1.2:59367509-59367528 None:intergenic 20.0%
!! ATTATATAGACACAACATAT+TGG + chr1.2:59367608-59367627 None:intergenic 20.0%
!! TAATATAAATTAGTCAAGAG+AGG - chr1.2:59367624-59367643 MS.gene40779:CDS 20.0%
!! TGAAATGAAATTAAATCACT+CGG - chr1.2:59368060-59368079 MS.gene40779:CDS 20.0%
!! TTTATACCAAACATTTAAAG+AGG + chr1.2:59367224-59367243 None:intergenic 20.0%
!!! GATTTGTTAAATGAGTTTAA+AGG - chr1.2:59368558-59368577 MS.gene40779:intron 20.0%
! AAACATGCACTAAAAGAAAA+AGG - chr1.2:59367051-59367070 MS.gene40779:CDS 25.0%
! AAATGCCTTGTAAAAAACAT+TGG - chr1.2:59368016-59368035 MS.gene40779:intron 25.0%
! AACTCATTTAACAAATCACT+TGG + chr1.2:59368555-59368574 None:intergenic 25.0%
! AAGACAAAGAAAAGAAGAAA+CGG - chr1.2:59368706-59368725 MS.gene40779:intron 25.0%
! ATAAAAAATGTAGTTGGAGA+AGG - chr1.2:59367095-59367114 MS.gene40779:CDS 25.0%
! ATCATTGACAATAAACAGAT+GGG - chr1.2:59369013-59369032 MS.gene40779:intron 25.0%
! ATGCTTGATATGTAGATATT+TGG - chr1.2:59367949-59367968 MS.gene40779:intron 25.0%
! CAAATTAAATCATGTGATCA+TGG + chr1.2:59367510-59367529 None:intergenic 25.0%
! GAAGATAAAGGAAAAAAAGA+AGG - chr1.2:59368982-59369001 MS.gene40779:intron 25.0%
! TGTTTGGATATTTGGATTTA+CGG - chr1.2:59366956-59366975 MS.gene40779:intron 25.0%
! TTGTGGATAAAAAATGTAGT+TGG - chr1.2:59367089-59367108 MS.gene40779:CDS 25.0%
! TTTGGATTCTTAATTAAAGC+AGG - chr1.2:59368618-59368637 MS.gene40779:intron 25.0%
! TTTGTTCCTCTTTAAATGTT+TGG - chr1.2:59367215-59367234 MS.gene40779:CDS 25.0%
!! ATAAAGTAACAAACCTGTTT+TGG + chr1.2:59368488-59368507 None:intergenic 25.0%
!! TACAACACATTTCAGTTTTA+AGG + chr1.2:59367194-59367213 None:intergenic 25.0%
!! TTTTTCTTTTAGTGCATGTT+TGG + chr1.2:59367052-59367071 None:intergenic 25.0%
!!! TACTGGTTTATCTCAATTAA+TGG - chr1.2:59368236-59368255 MS.gene40779:intron 25.0%
!!! TTGTTTTCTTTGTCATTTGA+GGG + chr1.2:59368681-59368700 None:intergenic 25.0%
AATCCTTATAACCTGTCATT+TGG + chr1.2:59368311-59368330 None:intergenic 30.0%
AATGAAATTAAATCACTCGG+TGG - chr1.2:59368063-59368082 MS.gene40779:CDS 30.0%
AATTGATCTCTTAGATACCT+TGG + chr1.2:59367880-59367899 None:intergenic 30.0%
AGAAAAGAAAGGAGACTTTA+AGG + chr1.2:59368165-59368184 None:intergenic 30.0%
AGAATGTGCTTCAATTTAGA+AGG - chr1.2:59367262-59367281 MS.gene40779:intron 30.0%
AGACATGCATTTAGTCATAT+TGG - chr1.2:59367167-59367186 MS.gene40779:CDS 30.0%
AGTAAGTATCCAAATCAACT+GGG + chr1.2:59369225-59369244 None:intergenic 30.0%
ATTGATCTCTTAGATACCTT+GGG + chr1.2:59367879-59367898 None:intergenic 30.0%
GAATGTGCTTCAATTTAGAA+GGG - chr1.2:59367263-59367282 MS.gene40779:intron 30.0%
GATCATTGACAATAAACAGA+TGG - chr1.2:59369012-59369031 MS.gene40779:intron 30.0%
GATTACTAAAGTTCCAAAAC+AGG - chr1.2:59368472-59368491 MS.gene40779:CDS 30.0%
GTATCTAAGAGATCAATTTG+TGG - chr1.2:59367882-59367901 MS.gene40779:intron 30.0%
GTTCGTTGGATATGTTAAAT+GGG - chr1.2:59369245-59369264 MS.gene40779:CDS 30.0%
TATTTGATATCCACTTTGTC+CGG + chr1.2:59368745-59368764 None:intergenic 30.0%
TGATGAAGATAATCTGAATG+TGG - chr1.2:59368260-59368279 MS.gene40779:intron 30.0%
TGTTCGTTGGATATGTTAAA+TGG - chr1.2:59369244-59369263 MS.gene40779:CDS 30.0%
TTGAGAAATTGTACTTCTAC+AGG - chr1.2:59367834-59367853 MS.gene40779:intron 30.0%
TTGATCCACTGATATGTAAA+AGG - chr1.2:59368354-59368373 MS.gene40779:intron 30.0%
TTGTGGCAAAAACTACAAAT+TGG + chr1.2:59367023-59367042 None:intergenic 30.0%
TTGTTGACAGAAGAATAGTT+TGG + chr1.2:59369145-59369164 None:intergenic 30.0%
! ATGTCTCGATTTTTCTTTTG+TGG + chr1.2:59367153-59367172 None:intergenic 30.0%
! ATTGGTCTAGGAAAATGTAT+TGG - chr1.2:59368034-59368053 MS.gene40779:CDS 30.0%
! CTTGTAAAAAACATTGGTCT+AGG - chr1.2:59368022-59368041 MS.gene40779:intron 30.0%
! GTAGATATCTTGTTTCATTC+TGG - chr1.2:59367914-59367933 MS.gene40779:intron 30.0%
! TGTCAAACTAACTGAAGTTT+TGG - chr1.2:59366750-59366769 MS.gene40779:CDS 30.0%
! TTCAGTTAGTTTGACATAGA+AGG + chr1.2:59366746-59366765 None:intergenic 30.0%
!! CTTGTTTTCTTTGTCATTTG+AGG + chr1.2:59368682-59368701 None:intergenic 30.0%
!! TTATCATACCTGTTTTTCAG+TGG + chr1.2:59368868-59368887 None:intergenic 30.0%
!!! CTAGACCAATGTTTTTTACA+AGG + chr1.2:59368024-59368043 None:intergenic 30.0%
AAGATCTTAAGGAGGAAAAG+AGG - chr1.2:59369074-59369093 MS.gene40779:intron 35.0%
ACAGAAGAATAGTTTGGTAG+TGG + chr1.2:59369139-59369158 None:intergenic 35.0%
ACATATTGGATCGATAGACT+TGG + chr1.2:59367594-59367613 None:intergenic 35.0%
AGATAATCTGAATGTGGCAA+AGG - chr1.2:59368266-59368285 MS.gene40779:intron 35.0%
AGGTCAATATGTGGATAACA+GGG + chr1.2:59367486-59367505 None:intergenic 35.0%
ATCCAAATATCCAAACATGC+AGG + chr1.2:59366953-59366972 None:intergenic 35.0%
ATGGCTACATGAAAAAGTAG+AGG + chr1.2:59368946-59368965 None:intergenic 35.0%
CAGTAAGTATCCAAATCAAC+TGG + chr1.2:59369226-59369245 None:intergenic 35.0%
CTTTACAAAATGTAGGTGTG+AGG + chr1.2:59368188-59368207 None:intergenic 35.0%
GGTGTATCTTGTGAATTTAG+AGG - chr1.2:59368579-59368598 MS.gene40779:intron 35.0%
GTCAAGTAGAATTGAAACAG+AGG + chr1.2:59367378-59367397 None:intergenic 35.0%
TAGCCAAATGACAGGTTATA+AGG - chr1.2:59368305-59368324 MS.gene40779:intron 35.0%
TCACTTCTCATTAATGCATG+TGG - chr1.2:59367547-59367566 MS.gene40779:intron 35.0%
TCGATTAAAAGCTCAAGATC+CGG - chr1.2:59368785-59368804 MS.gene40779:intron 35.0%
TGCATGTTTGGATGAATTTG+TGG + chr1.2:59367040-59367059 None:intergenic 35.0%
TGCTTCAATTTAGAAGGGAA+AGG - chr1.2:59367268-59367287 MS.gene40779:intron 35.0%
TGTACTTATTAGCTGCAATG+TGG - chr1.2:59368507-59368526 MS.gene40779:intron 35.0%
TGTGTGCATTATGCTATGTT+TGG - chr1.2:59366873-59366892 MS.gene40779:CDS 35.0%
TTAAATCATGTGATCATGGG+AGG + chr1.2:59367506-59367525 None:intergenic 35.0%
TTCCTGCATGTTTGGATATT+TGG - chr1.2:59366948-59366967 MS.gene40779:intron 35.0%
TTCTTAATTAAAGCAGGAGC+CGG - chr1.2:59368624-59368643 MS.gene40779:intron 35.0%
TTTGGATACTTACTGTTCGT+TGG - chr1.2:59369231-59369250 MS.gene40779:CDS 35.0%
! ACAAGTCTAAGATTGTCTCT+CGG + chr1.2:59367412-59367431 None:intergenic 35.0%
! AGACTTGTACCTGTTTTACT+TGG - chr1.2:59367424-59367443 MS.gene40779:intron 35.0%
! CTCACACCTACATTTTGTAA+AGG - chr1.2:59368186-59368205 MS.gene40779:intron 35.0%
! GCATAGATCTTTTCAACTTG+AGG + chr1.2:59367797-59367816 None:intergenic 35.0%
! TGCAACCTTTTACATATCAG+TGG + chr1.2:59368362-59368381 None:intergenic 35.0%
! TTTGTAAAGGTACGCGTAAA+AGG - chr1.2:59368199-59368218 MS.gene40779:intron 35.0%
!! AATGACTGAAAAAGCACTCA+AGG - chr1.2:59369189-59369208 MS.gene40779:CDS 35.0%
!! AGGTTGGTCTTCAAAACAAA+TGG - chr1.2:59367720-59367739 MS.gene40779:intron 35.0%
!! CTTTTTCATGTAGCCATATG+TGG - chr1.2:59368949-59368968 MS.gene40779:intron 35.0%
!! GGTTGGTCTTCAAAACAAAT+GGG - chr1.2:59367721-59367740 MS.gene40779:intron 35.0%
!!! ACTTTTTTGCCATCAATGTC+CGG + chr1.2:59368807-59368826 None:intergenic 35.0%
!!! ATTTGTTTTGAAGACCAACC+TGG + chr1.2:59367721-59367740 None:intergenic 35.0%
AAAGAAAAGAAGAAACGGCG+AGG - chr1.2:59368711-59368730 MS.gene40779:intron 40.0%
ACCAATGGAATTGTGTCCAA+TGG - chr1.2:59366682-59366701 MS.gene40779:CDS 40.0%
AGTTGTCTCTGCACTCAAAT+TGG - chr1.2:59367748-59367767 MS.gene40779:intron 40.0%
ATATCCAAACATGCAGGAAG+TGG + chr1.2:59366947-59366966 None:intergenic 40.0%
ATCCAAATCAACTGGGAAGA+AGG + chr1.2:59369218-59369237 None:intergenic 40.0%
CACAATTCCATTGGTGAGTT+TGG + chr1.2:59366677-59366696 None:intergenic 40.0%
CACTAGCTAGTCATAGTCAT+CGG - chr1.2:59367119-59367138 MS.gene40779:CDS 40.0%
CCAATGGAATTGTGTCCAAT+GGG - chr1.2:59366683-59366702 MS.gene40779:CDS 40.0%
CGCGTACCTTTACAAAATGT+AGG + chr1.2:59368195-59368214 None:intergenic 40.0%
GAGGTCAATATGTGGATAAC+AGG + chr1.2:59367487-59367506 None:intergenic 40.0%
GGAGGAAAGCAAGAAGATAA+AGG - chr1.2:59368970-59368989 MS.gene40779:intron 40.0%
GGATTCTCTTGAATTTGTCC+AGG - chr1.2:59367700-59367719 MS.gene40779:intron 40.0%
GGTGCTTATGCTGCAATATT+TGG - chr1.2:59368600-59368619 MS.gene40779:intron 40.0%
GTCTCTATCTCTAAGTCATC+AGG + chr1.2:59366724-59366743 None:intergenic 40.0%
TAGGTGTGAGGAGAAAAGAA+AGG + chr1.2:59368176-59368195 None:intergenic 40.0%
TCCAAATCAACTGGGAAGAA+GGG + chr1.2:59369217-59369236 None:intergenic 40.0%
TCTCTTGAATTTGTCCAGGT+TGG - chr1.2:59367704-59367723 MS.gene40779:intron 40.0%
TGGTGACTTCCAAGTAAAAC+AGG + chr1.2:59367436-59367455 None:intergenic 40.0%
TTCTTGCTTTCCTCCACATA+TGG + chr1.2:59368965-59368984 None:intergenic 40.0%
TTTCATGTAGCCATATGTGG+AGG - chr1.2:59368952-59368971 MS.gene40779:intron 40.0%
! AGAATTGAAACAGAGGTGCA+TGG + chr1.2:59367371-59367390 None:intergenic 40.0%
! GGAAAAAGTGTGTGTACTTG+TGG - chr1.2:59367072-59367091 MS.gene40779:CDS 40.0%
!! AGAAGAGTACTGTTTCCCAT+TGG + chr1.2:59366701-59366720 None:intergenic 40.0%
!! GAAGAGCACTCAAGATCTTA+AGG - chr1.2:59369063-59369082 MS.gene40779:intron 40.0%
AGACATGAGATTGAACCCAG+AGG + chr1.2:59366779-59366798 None:intergenic 45.0%
ATCGAGCACATTCATGCTTG+AGG + chr1.2:59368770-59368789 None:intergenic 45.0%
CATTTGGCTAGAGTAGTCCT+TGG + chr1.2:59368295-59368314 None:intergenic 45.0%
CCCATTGGACACAATTCCAT+TGG + chr1.2:59366686-59366705 None:intergenic 45.0%
GAATGTGGCAAAGGTTTCCA+AGG - chr1.2:59368275-59368294 MS.gene40779:intron 45.0%
GACTACTCTAGCCAAATGAC+AGG - chr1.2:59368297-59368316 MS.gene40779:intron 45.0%
GAGCACTCAAGATCTTAAGG+AGG - chr1.2:59369066-59369085 MS.gene40779:intron 45.0%
GATCATGGGAGGTCAATATG+TGG + chr1.2:59367495-59367514 None:intergenic 45.0%
GATGTCTGCCACTGAAAAAC+AGG - chr1.2:59368857-59368876 MS.gene40779:intron 45.0%
GTCTCTGCACTCAAATTGGA+AGG - chr1.2:59367752-59367771 MS.gene40779:intron 45.0%
GTGGTGACACAAGTGTACAT+CGG - chr1.2:59368082-59368101 MS.gene40779:CDS 45.0%
TACTTGGAAGTCACCAGAAG+AGG - chr1.2:59367440-59367459 MS.gene40779:intron 45.0%
TCAGATGCCAAACTCACCAA+TGG - chr1.2:59366667-59366686 MS.gene40779:CDS 45.0%
TCCCTTCTTCCCAGTTGATT+TGG - chr1.2:59369213-59369232 MS.gene40779:CDS 45.0%
TGATATCCACTTTGTCCGGT+AGG + chr1.2:59368741-59368760 None:intergenic 45.0%
TGGATAACAGGGCAAATACC+TGG + chr1.2:59367475-59367494 None:intergenic 45.0%
TGGCAAAAAAGTCCTACGCA+TGG - chr1.2:59368815-59368834 MS.gene40779:intron 45.0%
TTCTACAGGTTTCCTGCAAC+CGG - chr1.2:59367848-59367867 MS.gene40779:intron 45.0%
TTCTGTCAACAATGCAGCTG+TGG - chr1.2:59369153-59369172 MS.gene40779:CDS 45.0%
TTGGCATCTGAAGGTGATGA+TGG + chr1.2:59366658-59366677 None:intergenic 45.0%
TTGGTGAGTTTGGCATCTGA+AGG + chr1.2:59366667-59366686 None:intergenic 45.0%
! ATTGCCACTTCCTGCATGTT+TGG - chr1.2:59366940-59366959 MS.gene40779:intron 45.0%
!! GGGCACTGAAGATCTTAAGA+AGG - chr1.2:59369033-59369052 MS.gene40779:intron 45.0%
!!! ACTGAAGTTTTGGACTCCTC+TGG - chr1.2:59366760-59366779 MS.gene40779:CDS 45.0%
!!! CTGAAGTTTTGGACTCCTCT+GGG - chr1.2:59366761-59366780 MS.gene40779:CDS 45.0%
AAGCATCAACAGCCATGCGT+AGG + chr1.2:59368830-59368849 None:intergenic 50.0%
AGGAAGCAGAACTCGACTAC+TGG - chr1.2:59368219-59368238 MS.gene40779:intron 50.0%
CAACAATGCAGCTGTGGAGT+TGG - chr1.2:59369159-59369178 MS.gene40779:CDS 50.0%
CTGGTGAAAACCTCCTCTTC+TGG + chr1.2:59367456-59367475 None:intergenic 50.0%
GAAAAGAGGCCTAGTGTGTG+CGG - chr1.2:59369088-59369107 MS.gene40779:intron 50.0%
GCTCAAGATCCGGACATTGA+TGG - chr1.2:59368795-59368814 MS.gene40779:intron 50.0%
TACGCATGGCTGTTGATGCT+TGG - chr1.2:59368829-59368848 MS.gene40779:intron 50.0%
TAGTCGTAACCGCACACACT+AGG + chr1.2:59369100-59369119 None:intergenic 50.0%
TATCCACTTTGTCCGGTAGG+AGG + chr1.2:59368738-59368757 None:intergenic 50.0%
TTGGAAGTCACCAGAAGAGG+AGG - chr1.2:59367443-59367462 MS.gene40779:intron 50.0%
! CAGAAGAGGAGGTTTTCACC+AGG - chr1.2:59367454-59367473 MS.gene40779:intron 50.0%
! TTAGATACCTTGGGCGCTGC+CGG + chr1.2:59367870-59367889 None:intergenic 55.0%
AAACGGCGAGGTCCTCCTAC+CGG - chr1.2:59368723-59368742 MS.gene40779:intron 60.0%
AGCCGGCTGTCAAGAACCTC+GGG - chr1.2:59368641-59368660 MS.gene40779:intron 60.0%
CACCCGAGGTTCTTGACAGC+CGG + chr1.2:59368646-59368665 None:intergenic 60.0%
GAGCCGGCTGTCAAGAACCT+CGG - chr1.2:59368640-59368659 MS.gene40779:intron 60.0%
GGTCCTCCTACCGGACAAAG+TGG - chr1.2:59368732-59368751 MS.gene40779:intron 60.0%
!! GGTGCTTGTAGAAGCACCCG+AGG + chr1.2:59368660-59368679 None:intergenic 60.0%
CCTGCAACCGGCAGCGCCCA+AGG - chr1.2:59367860-59367879 MS.gene40779:intron 75.0%
! CCTTGGGCGCTGCCGGTTGC+AGG + chr1.2:59367863-59367882 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 59366631 59369279 59366631 ID=MS.gene40779
chr1.2 mRNA 59366631 59369279 59366631 ID=MS.gene40779.t1;Parent=MS.gene40779
chr1.2 exon 59369116 59369279 59369116 ID=MS.gene40779.t1.exon1;Parent=MS.gene40779.t1
chr1.2 CDS 59369116 59369279 59369116 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
chr1.2 exon 59368435 59368510 59368435 ID=MS.gene40779.t1.exon2;Parent=MS.gene40779.t1
chr1.2 CDS 59368435 59368510 59368435 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
chr1.2 exon 59368029 59368188 59368029 ID=MS.gene40779.t1.exon3;Parent=MS.gene40779.t1
chr1.2 CDS 59368029 59368188 59368029 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
chr1.2 exon 59367592 59367702 59367592 ID=MS.gene40779.t1.exon4;Parent=MS.gene40779.t1
chr1.2 CDS 59367592 59367702 59367592 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
chr1.2 exon 59367032 59367273 59367032 ID=MS.gene40779.t1.exon5;Parent=MS.gene40779.t1
chr1.2 CDS 59367032 59367273 59367032 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
chr1.2 exon 59366631 59366948 59366631 ID=MS.gene40779.t1.exon6;Parent=MS.gene40779.t1
chr1.2 CDS 59366631 59366948 59366631 ID=cds.MS.gene40779.t1;Parent=MS.gene40779.t1
Gene Sequence

>MS.gene40779

ATGGAAGGCAACAAAACTATGGTTCCATCATCACCTTCAGATGCCAAACTCACCAATGGAATTGTGTCCAATGGGAAACAGTACTCTTCTCCTGATGACTTAGAGATAGAGACCTTCTATGTCAAACTAACTGAAGTTTTGGACTCCTCTGGGTTCAATCTCATGTCTGTTTCTTCAATCAGCTTCTTACTTTCTGTTTTGCTTTTATTTTTTTCGTTTGTATTTGCATGAGTGTTTTTACTTGTGTGCATTATGCTATGTTTGGTTTTACAAAATTTGCATGCAAAAAACGTCAAGCATTTTTTTTTAATTGCCACTTCCTGCATGTTTGGATATTTGGATTTACGGTAAATTTGTAAAAATAACAGTGACATACTCGATTTTATTCTCCAATTTGTAGTTTTTGCCACAAATTCATCCAAACATGCACTAAAAGAAAAAGGAAAAAGTGTGTGTACTTGTGGATAAAAAATGTAGTTGGAGAAGGTCACTAGCTAGTCATAGTCATCGGCAAAGTCACCACAAAAGAAAAATCGAGACATGCATTTAGTCATATTGGTCCTTAAAACTGAAATGTGTTGTAATTTGTTCCTCTTTAAATGTTTGGTATAAAGAATGTCACTAGAATAGTAGAATGTGCTTCAATTTAGAAGGGAAAGGTGATGTGATACATGAAACATGAAATGTTATTGACTTTATTTTATATAAAGTTTTATGAGATAATAAAGGGTATATGACCATGCACCTCTGTTTCAATTCTACTTGACAGTTTCAATGTCCGAGAGACAATCTTAGACTTGTACCTGTTTTACTTGGAAGTCACCAGAAGAGGAGGTTTTCACCAGGTATTTGCCCTGTTATCCACATATTGACCTCCCATGATCACATGATTTAATTTGTTGTATTTGTTATATGTTCACTTCTCATTAATGCATGTGGCTTCTATTATTTGCATGCACACCAAGTCTATCGATCCAATATGTTGTGTCTATATAATATAAATTAGTCAAGAGAGGCTTTTAATTAAGTAATTTTTCATATGAATTGTTCAGATTTAATATATTGTAATGGATTCTCTTGAATTTGTCCAGGTTGGTCTTCAAAACAAATGGGCTGAAGTTGTCTCTGCACTCAAATTGGAAGGCAACAATGCAATGTTATCTCCTCAAGTTGAAAAGATCTATGCAACACTTCTATACAAATTTGAGAAATTGTACTTCTACAGGTTTCCTGCAACCGGCAGCGCCCAAGGTATCTAAGAGATCAATTTGTGGTTATTTTAAGTAGATATCTTGTTTCATTCTGGTACTTCACTTAGATGCTTGATATGTAGATATTTGGTAGTTTATACATAATATGATTTTAATTTACAACTAGTAATAGAAAAATGCCTTGTAAAAAACATTGGTCTAGGAAAATGTATTGGATATGAAATGAAATTAAATCACTCGGTGGTGACACAAGTGTACATCGGATCATTGTATGATGTTTTTATTACATTTTTGTTTTAAGATGAAAATGTGTTGTTTAACCTTAAAGTCTCCTTTCTTTTCTCCTCACACCTACATTTTGTAAAGGTACGCGTAAAAGGAAGCAGAACTCGACTACTGGTTTATCTCAATTAATGGCTGATGAAGATAATCTGAATGTGGCAAAGGTTTCCAAGGACTACTCTAGCCAAATGACAGGTTATAAGGATTTAGAATATTTATGCAATGAAAATTTGATCCACTGATATGTAAAAGGTTGCACTCAAGTACTAATGAATTAACTAGCTAACTAGCTGAATCAAAACTAATATCACAACATAACACAATTTATCAACTAATCATGATAACTGAGATTACTAAAGTTCCAAAACAGGTTTGTTACTTTATGTACTTATTAGCTGCAATGTGGACATTTTTTTTCTTTAGTAACTCCAAGTGATTTGTTAAATGAGTTTAAAGGTGTATCTTGTGAATTTAGAGGTGCTTATGCTGCAATATTTGGATTCTTAATTAAAGCAGGAGCCGGCTGTCAAGAACCTCGGGTGCTTCTACAAGCACCCTCAAATGACAAAGAAAACAAGAAACAAGACAAAGAAAAGAAGAAACGGCGAGGTCCTCCTACCGGACAAAGTGGATATCAAATATTCCTCAAGCATGAATGTGCTCGATTAAAAGCTCAAGATCCGGACATTGATGGCAAAAAAGTCCTACGCATGGCTGTTGATGCTTGGCAGAAGATGTCTGCCACTGAAAAACAGGTATGATAAATAAAATTGACATAATATCGATCAAATATCGAACAATTTATATTTTTGCAATTAGCCTCTACTTTTTCATGTAGCCATATGTGGAGGAAAGCAAGAAGATAAAGGAAAAAAAGAAGGAAGCAGTGATCATTGACAATAAACAGATGGGCACTGAAGATCTTAAGAAGGATGAAAAGAAGAGCACTCAAGATCTTAAGGAGGAAAAGAGGCCTAGTGTGTGCGGTTACGACTACTGTGCAACTTCGCAACCACTACCAAACTATTCTTCTGTCAACAATGCAGCTGTGGAGTTGGCTTTGAAAATGACTGAAAAAGCACTCAAGGATCCCTTCTTCCCAGTTGATTTGGATACTTACTGTTCGTTGGATATGTTAAATGGGAAATCCAAATGA
Protein sequence

>MS.gene40779.t1

MEGNKTMVPSSPSDAKLTNGIVSNGKQYSSPDDLEIETFYVKLTEVLDSSGFNLIFNVRETILDLYLFYLEVTRRGGFHQVGLQNKWAEVVSALKLEGNNAMLSPQVEKIYATLLYKFEKLYFYRFPATGSAQGTRKRKQNSTTGLSQLMADEDNLNVAKVSKDYSSQMTAGAGCQEPRVLLQAPSNDKENKKQDKEKKKRRGPPTGQSGYQIFLKHECARLKAQDPDIDGKKVLRMAVDAWQKMSATEKQPYVEESKKIKEKKKEAVIIDNKQMGTEDLKKDEKKSTQDLKEEKRPSVCGYDYCATSQPLPNYSSVNNAAVELALKMTEKALKDPFFPVDLDTYCSLDMLNGKSK