Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40889.t1 | XP_004513765.1 | 87 | 54 | 7 | 0 | 1 | 54 | 195 | 248 | 5.00E-18 | 100.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40889.t1 | Q8VZ42 | 52.7 | 55 | 21 | 1 | 1 | 55 | 190 | 239 | 3.1e-08 | 58.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40889.t1 | A0A392R514 | 89.5 | 57 | 6 | 0 | 1 | 57 | 59 | 115 | 1.0e-20 | 108.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene40889.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40889.t1 | MTR_0100s0160 | 91.011 | 89 | 2 | 1 | 1 | 83 | 193 | 281 | 7.37e-52 | 163 |
| MS.gene40889.t1 | MTR_7g091810 | 59.701 | 67 | 19 | 1 | 25 | 83 | 198 | 264 | 5.70e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40889.t1 | AT2G41835 | 50.562 | 89 | 33 | 2 | 1 | 83 | 190 | 273 | 4.78e-24 | 91.7 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAGTGATTGAGGAAAACT+TGG | 0.473429 | 1.4:-44052168 | None:intergenic |
| CACAGATCCCGTGTCCCTTG+TGG | 0.520415 | 1.4:+44052268 | MS.gene40889:CDS |
| GCGTGTCCTAAGTGTAGTAA+AGG | 0.526636 | 1.4:+44052242 | MS.gene40889:CDS |
| CCCTCTCAACATGCTCCACA+AGG | 0.529390 | 1.4:-44052283 | None:intergenic |
| AAAACTTGGCACCACATTGA+GGG | 0.531828 | 1.4:-44052154 | None:intergenic |
| GTGAATCCTTTACTACACTT+AGG | 0.538962 | 1.4:-44052248 | None:intergenic |
| ATCGATTAAAGTAGTGATTG+AGG | 0.539536 | 1.4:-44052177 | None:intergenic |
| GTGGTGAAATTAACCAAGCA+TGG | 0.560055 | 1.4:+44052075 | MS.gene40889:CDS |
| TGGTGAAATTAACCAAGCAT+GGG | 0.560973 | 1.4:+44052076 | MS.gene40889:CDS |
| GAAAACTTGGCACCACATTG+AGG | 0.568886 | 1.4:-44052155 | None:intergenic |
| AACATGCTCCACAAGGGACA+CGG | 0.573079 | 1.4:-44052276 | None:intergenic |
| GAGCAACGACAGTGGTCAAG+TGG | 0.579833 | 1.4:+44052124 | MS.gene40889:CDS |
| ACATGCTCCACAAGGGACAC+GGG | 0.604172 | 1.4:-44052275 | None:intergenic |
| ACTTAGGACACGCATCAGAT+CGG | 0.613401 | 1.4:-44052232 | None:intergenic |
| GAGCATGTTGAGAGGGAACA+TGG | 0.625868 | 1.4:+44052290 | MS.gene40889:CDS |
| CCCTTGTGGAGCATGTTGAG+AGG | 0.626858 | 1.4:+44052282 | MS.gene40889:CDS |
| CCTTGTGGAGCATGTTGAGA+GGG | 0.637426 | 1.4:+44052283 | MS.gene40889:CDS |
| GGGAGAAAGAGCAACGACAG+TGG | 0.676386 | 1.4:+44052116 | MS.gene40889:CDS |
| GGTGAAATTAACCAAGCATG+GGG | 0.687152 | 1.4:+44052077 | MS.gene40889:CDS |
| CCTCTCAACATGCTCCACAA+GGG | 0.702395 | 1.4:-44052282 | None:intergenic |
| GCTGGAATGTCAAAGTTGAG+TGG | 0.723894 | 1.4:+44052056 | None:intergenic |
| GTGAAATTAACCAAGCATGG+GGG | 0.745364 | 1.4:+44052078 | MS.gene40889:CDS |
| GTGGAGCAATGCCCTCAATG+TGG | 0.769864 | 1.4:+44052143 | MS.gene40889:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCGATTAAAGTAGTGATTG+AGG | - | chr1.4:44052180-44052199 | None:intergenic | 30.0% | |
| CAAAAAGTTCATGAAAGAAG+TGG | + | chr1.4:44052206-44052225 | MS.gene40889:CDS | 30.0% | |
| AGTAGTGATTGAGGAAAACT+TGG | - | chr1.4:44052171-44052190 | None:intergenic | 35.0% | |
| GTGAATCCTTTACTACACTT+AGG | - | chr1.4:44052251-44052270 | None:intergenic | 35.0% | |
| TGGTGAAATTAACCAAGCAT+GGG | + | chr1.4:44052076-44052095 | MS.gene40889:CDS | 35.0% | |
| AAAACTTGGCACCACATTGA+GGG | - | chr1.4:44052157-44052176 | None:intergenic | 40.0% | |
| GGTGAAATTAACCAAGCATG+GGG | + | chr1.4:44052077-44052096 | MS.gene40889:CDS | 40.0% | |
| GTGAAATTAACCAAGCATGG+GGG | + | chr1.4:44052078-44052097 | MS.gene40889:CDS | 40.0% | |
| GTGGTGAAATTAACCAAGCA+TGG | + | chr1.4:44052075-44052094 | MS.gene40889:CDS | 40.0% | |
| ACTTAGGACACGCATCAGAT+CGG | - | chr1.4:44052235-44052254 | None:intergenic | 45.0% | |
| GAAAACTTGGCACCACATTG+AGG | - | chr1.4:44052158-44052177 | None:intergenic | 45.0% | |
| GCGTGTCCTAAGTGTAGTAA+AGG | + | chr1.4:44052242-44052261 | MS.gene40889:CDS | 45.0% | |
| AACATGCTCCACAAGGGACA+CGG | - | chr1.4:44052279-44052298 | None:intergenic | 50.0% | |
| CCTCTCAACATGCTCCACAA+GGG | - | chr1.4:44052285-44052304 | None:intergenic | 50.0% | |
| CCTTGTGGAGCATGTTGAGA+GGG | + | chr1.4:44052283-44052302 | MS.gene40889:CDS | 50.0% | |
| ACATGCTCCACAAGGGACAC+GGG | - | chr1.4:44052278-44052297 | None:intergenic | 55.0% | |
| CCCTCTCAACATGCTCCACA+AGG | - | chr1.4:44052286-44052305 | None:intergenic | 55.0% | |
| CCCTTGTGGAGCATGTTGAG+AGG | + | chr1.4:44052282-44052301 | MS.gene40889:CDS | 55.0% | |
| GAGCAACGACAGTGGTCAAG+TGG | + | chr1.4:44052124-44052143 | MS.gene40889:CDS | 55.0% | |
| GGGAGAAAGAGCAACGACAG+TGG | + | chr1.4:44052116-44052135 | MS.gene40889:CDS | 55.0% | |
| GTGGAGCAATGCCCTCAATG+TGG | + | chr1.4:44052143-44052162 | MS.gene40889:CDS | 55.0% | |
| CACAGATCCCGTGTCCCTTG+TGG | + | chr1.4:44052268-44052287 | MS.gene40889:CDS | 60.0% | |
| CGCTTGATGTCCCCCATGCT+TGG | - | chr1.4:44052091-44052110 | None:intergenic | 60.0% | |
| GCATGGGGGACATCAAGCGA+TGG | + | chr1.4:44052092-44052111 | MS.gene40889:CDS | 60.0% | |
| GGGGGACATCAAGCGATGGT+GGG | + | chr1.4:44052096-44052115 | MS.gene40889:CDS | 60.0% | |
| TGGGGGACATCAAGCGATGG+TGG | + | chr1.4:44052095-44052114 | MS.gene40889:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 44052062 | 44052311 | 44052062 | ID=MS.gene40889 |
| chr1.4 | mRNA | 44052062 | 44052311 | 44052062 | ID=MS.gene40889.t1;Parent=MS.gene40889 |
| chr1.4 | exon | 44052062 | 44052311 | 44052062 | ID=MS.gene40889.t1.exon1;Parent=MS.gene40889.t1 |
| chr1.4 | CDS | 44052062 | 44052311 | 44052062 | ID=cds.MS.gene40889.t1;Parent=MS.gene40889.t1 |
| Gene Sequence |
| Protein sequence |
