Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41102.t1 | KEH41952.1 | 92.3 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 1.60E-38 | 168.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41102.t1 | A0A072VJI7 | 92.3 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 1.2e-38 | 168.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene41102.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41102.t1 | MTR_1g058090 | 92.308 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 6.91e-58 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAGTGATCATTTCTTTCA+TGG | 0.293801 | 1.3:-42650871 | None:intergenic |
| AGGAGGAGAAGAAATTGATT+TGG | 0.338250 | 1.3:+42650947 | MS.gene41102:CDS |
| TCTCCTCCTTGACTTGGACT+TGG | 0.356989 | 1.3:-42650933 | None:intergenic |
| ATTTCTTCTCCTCCTTGACT+TGG | 0.410406 | 1.3:-42650939 | None:intergenic |
| TCCTTGACTTGGACTTGGAT+TGG | 0.422024 | 1.3:-42650928 | None:intergenic |
| GACTCTCATGTGACCATTCA+AGG | 0.426545 | 1.3:-42650826 | None:intergenic |
| AGACTTATAACTTGAGGTCT+AGG | 0.432547 | 1.3:-42651016 | None:intergenic |
| TCAAGAGTGAATGCCTTGAA+TGG | 0.452902 | 1.3:+42650813 | MS.gene41102:CDS |
| GTGCTAGGAGTAGCCTTGGT+TGG | 0.456915 | 1.3:-42650984 | None:intergenic |
| GGCTACTCCTAGCACTAAGT+TGG | 0.507989 | 1.3:+42650992 | MS.gene41102:CDS |
| CTTCATAGACTTATAACTTG+AGG | 0.541664 | 1.3:-42651022 | None:intergenic |
| CTTAGTGCTAGGAGTAGCCT+TGG | 0.543078 | 1.3:-42650988 | None:intergenic |
| TTTGGTTCTTCGCCTCATGT+TGG | 0.548092 | 1.3:-42650899 | None:intergenic |
| ATGAATTTCCCTATGATCCA+TGG | 0.571015 | 1.3:-42650763 | None:intergenic |
| ATTGATTTGGAACTAAGACT+TGG | 0.574413 | 1.3:+42650960 | MS.gene41102:CDS |
| TCTAGGTCCAACTTAGTGCT+AGG | 0.576797 | 1.3:-42650999 | None:intergenic |
| ATGGTCACATGAGAGTCCAC+AGG | 0.587507 | 1.3:+42650832 | MS.gene41102:CDS |
| ACCAATCCAAGTCCAAGTCA+AGG | 0.611700 | 1.3:+42650927 | MS.gene41102:CDS |
| TGGTCACATGAGAGTCCACA+GGG | 0.650216 | 1.3:+42650833 | MS.gene41102:CDS |
| CACTACTCTCAACCAACATG+AGG | 0.659174 | 1.3:+42650887 | MS.gene41102:CDS |
| ACTAAGACTTGGTCCAACCA+AGG | 0.676612 | 1.3:+42650971 | MS.gene41102:CDS |
| AATCCAAGTCCAAGTCAAGG+AGG | 0.729846 | 1.3:+42650930 | MS.gene41102:CDS |
| TCACATGAGAGTCCACAGGG+AGG | 0.780305 | 1.3:+42650836 | MS.gene41102:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTAGTGATCATTTCTTTCA+TGG | - | chr1.3:42650874-42650893 | None:intergenic | 30.0% | |
| !! | ATTGATTTGGAACTAAGACT+TGG | + | chr1.3:42650960-42650979 | MS.gene41102:CDS | 30.0% |
| AGGAGGAGAAGAAATTGATT+TGG | + | chr1.3:42650947-42650966 | MS.gene41102:CDS | 35.0% | |
| CATTCACTCTTGAAAAAGTC+AGG | - | chr1.3:42650806-42650825 | None:intergenic | 35.0% | |
| ATTTCTTCTCCTCCTTGACT+TGG | - | chr1.3:42650942-42650961 | None:intergenic | 40.0% | |
| TCAAGAGTGAATGCCTTGAA+TGG | + | chr1.3:42650813-42650832 | MS.gene41102:CDS | 40.0% | |
| !!! | GACTTGGATTGGTGACTTTT+TGG | - | chr1.3:42650920-42650939 | None:intergenic | 40.0% |
| AATCCAAGTCCAAGTCAAGG+AGG | + | chr1.3:42650930-42650949 | MS.gene41102:CDS | 45.0% | |
| ACCAATCCAAGTCCAAGTCA+AGG | + | chr1.3:42650927-42650946 | MS.gene41102:CDS | 45.0% | |
| CACTACTCTCAACCAACATG+AGG | + | chr1.3:42650887-42650906 | MS.gene41102:CDS | 45.0% | |
| CCACAGGGAGGAGAAAAATA+AGG | + | chr1.3:42650848-42650867 | MS.gene41102:CDS | 45.0% | |
| GACTCTCATGTGACCATTCA+AGG | - | chr1.3:42650829-42650848 | None:intergenic | 45.0% | |
| TCTAGGTCCAACTTAGTGCT+AGG | - | chr1.3:42651002-42651021 | None:intergenic | 45.0% | |
| ! | ACTAAGACTTGGTCCAACCA+AGG | + | chr1.3:42650971-42650990 | MS.gene41102:CDS | 45.0% |
| !! | TCCTTGACTTGGACTTGGAT+TGG | - | chr1.3:42650931-42650950 | None:intergenic | 45.0% |
| !! | TTTGGTTCTTCGCCTCATGT+TGG | - | chr1.3:42650902-42650921 | None:intergenic | 45.0% |
| !!! | CCTTATTTTTCTCCTCCCTG+TGG | - | chr1.3:42650851-42650870 | None:intergenic | 45.0% |
| ATGGTCACATGAGAGTCCAC+AGG | + | chr1.3:42650832-42650851 | MS.gene41102:CDS | 50.0% | |
| CTTAGTGCTAGGAGTAGCCT+TGG | - | chr1.3:42650991-42651010 | None:intergenic | 50.0% | |
| GGCTACTCCTAGCACTAAGT+TGG | + | chr1.3:42650992-42651011 | MS.gene41102:CDS | 50.0% | |
| TGGTCACATGAGAGTCCACA+GGG | + | chr1.3:42650833-42650852 | MS.gene41102:CDS | 50.0% | |
| !! | TCTCCTCCTTGACTTGGACT+TGG | - | chr1.3:42650936-42650955 | None:intergenic | 50.0% |
| TCACATGAGAGTCCACAGGG+AGG | + | chr1.3:42650836-42650855 | MS.gene41102:CDS | 55.0% | |
| ! | GTGCTAGGAGTAGCCTTGGT+TGG | - | chr1.3:42650987-42651006 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 42650765 | 42651034 | 42650765 | ID=MS.gene41102 |
| chr1.3 | mRNA | 42650765 | 42651034 | 42650765 | ID=MS.gene41102.t1;Parent=MS.gene41102 |
| chr1.3 | exon | 42650765 | 42651034 | 42650765 | ID=MS.gene41102.t1.exon1;Parent=MS.gene41102.t1 |
| chr1.3 | CDS | 42650765 | 42651034 | 42650765 | ID=cds.MS.gene41102.t1;Parent=MS.gene41102.t1 |
| Gene Sequence |
| Protein sequence |
