AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41184

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41184.t1 MTR_4g063735 94.872 234 7 3 25 254 25 257 1.62e-164 455
MS.gene41184.t1 MTR_8g107210 56.383 94 35 2 12 99 80 173 6.78e-28 109
MS.gene41184.t1 MTR_4g084550 57.317 82 35 0 6 87 45 126 2.59e-27 103
MS.gene41184.t1 MTR_1g019130 62.500 72 27 0 20 91 85 156 6.26e-27 104
MS.gene41184.t1 MTR_1g019130 35.754 179 85 4 20 196 85 235 1.18e-26 103
MS.gene41184.t1 MTR_7g060630 66.667 66 22 0 23 88 6 71 6.81e-26 100
MS.gene41184.t1 MTR_5g021930 68.182 66 20 1 22 86 27 92 6.46e-25 100
MS.gene41184.t1 MTR_5g081990 51.899 79 37 1 13 90 20 98 3.73e-24 95.9
MS.gene41184.t1 MTR_8g095580 65.455 55 19 0 34 88 91 145 3.53e-20 84.7
MS.gene41184.t1 MTR_3g088485 63.636 55 20 0 34 88 89 143 7.07e-20 84.0
MS.gene41184.t1 MTR_2g015000 48.387 62 32 0 25 86 55 116 4.14e-14 71.6
MS.gene41184.t1 MTR_7g025010 45.161 62 34 0 25 86 47 108 2.28e-13 69.7
MS.gene41184.t1 MTR_1g115315 46.154 65 35 0 20 84 80 144 6.19e-13 67.0
MS.gene41184.t1 MTR_1g115315 44.776 67 37 0 20 86 80 146 7.51e-13 67.0
MS.gene41184.t1 MTR_4g088070 44.262 61 34 0 25 85 29 89 3.59e-12 65.9
MS.gene41184.t1 MTR_3g115620 40.909 66 39 0 20 85 77 142 1.11e-11 62.8
MS.gene41184.t1 MTR_7g023810 45.161 62 34 0 25 86 68 129 3.50e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41184.t1 AT5G59340 41.245 257 114 8 17 250 6 248 1.07e-53 174
MS.gene41184.t1 AT3G18010 45.312 128 57 2 20 134 71 198 5.77e-29 112
MS.gene41184.t1 AT1G46480 36.994 173 98 3 24 196 89 250 1.76e-26 103
MS.gene41184.t1 AT1G46480 65.672 67 23 0 24 90 89 155 3.24e-26 101
MS.gene41184.t1 AT2G28610 62.687 67 25 0 22 88 5 71 6.98e-25 99.4
MS.gene41184.t1 AT3G11260 50.000 80 40 0 13 92 12 91 8.24e-25 97.4
MS.gene41184.t1 AT5G05770 54.054 74 34 0 21 94 25 98 9.94e-25 95.5
MS.gene41184.t1 AT2G17950 63.636 66 23 1 22 86 35 100 2.19e-23 96.3
MS.gene41184.t1 AT2G01500 64.407 59 21 0 21 79 57 115 5.09e-22 92.4
MS.gene41184.t1 AT5G45980 51.613 62 30 0 25 86 55 116 7.81e-15 73.2
MS.gene41184.t1 AT1G20700 40.506 79 47 0 13 91 21 99 8.26e-15 70.1
MS.gene41184.t1 AT1G20700 40.506 79 47 0 13 91 83 161 1.94e-14 70.5
MS.gene41184.t1 AT1G20700 40.506 79 47 0 13 91 83 161 2.28e-14 70.5
MS.gene41184.t1 AT2G33880 48.387 62 32 0 25 86 55 116 9.06e-14 70.5
MS.gene41184.t1 AT2G33880 48.387 62 32 0 25 86 55 116 9.26e-14 70.5
MS.gene41184.t1 AT4G35550 40.278 72 43 0 14 85 88 159 3.18e-13 68.2
MS.gene41184.t1 AT1G20710 50.000 54 27 0 32 85 86 139 2.06e-11 62.0

Find 45 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATAGGTGAAGGATAAA+TGG 0.223363 4.1:+36826521 None:intergenic
AATCAAGATCATGGTTATAA+TGG 0.248654 4.1:-36825270 MS.gene41184:CDS
CTGTAACCAAAGTTATAGTT+AGG 0.305798 4.1:+36825438 None:intergenic
TTCTTCAGGACAAGGTTCTT+AGG 0.317828 4.1:-36825217 None:intergenic
AGTTCCACTGATACACATTC+TGG 0.341293 4.1:-36825315 MS.gene41184:CDS
AACATACCATTTGGACAAAT+AGG 0.347632 4.1:+36826507 None:intergenic
ACTGCATCCAACTGGCATAT+TGG 0.347683 4.1:-36825388 MS.gene41184:CDS
ACTGCAGGTAGAAGCACTTT+TGG 0.360138 4.1:+36825561 None:intergenic
TAGCTTATTTCATGACTTTG+TGG 0.366655 4.1:+36825597 None:intergenic
CCTTCACCTATTTGTCCAAA+TGG 0.376093 4.1:-36826513 MS.gene41184:intron
ATTAATGAGGAAGGTTCAAA+TGG 0.376394 4.1:-36826801 MS.gene41184:CDS
ATCAAGATCATGGTTATAAT+GGG 0.392817 4.1:-36825269 MS.gene41184:CDS
TGTGGTTGTGGTACGCAATA+TGG 0.424892 4.1:+36825615 None:intergenic
ATTTCATGACTTTGTGGTTG+TGG 0.425330 4.1:+36825603 None:intergenic
CAAATAGGTGAAGGATAAAT+GGG 0.425469 4.1:+36826522 None:intergenic
TCTTGTATTTCTTCAGCACT+TGG 0.437582 4.1:+36826687 None:intergenic
TTGTTTCCACTGCATCCAAC+TGG 0.438638 4.1:-36825396 MS.gene41184:CDS
AGAATCCCTAACTATAACTT+TGG 0.443156 4.1:-36825444 MS.gene41184:CDS
TTCCCTTCCAATATGCCAGT+TGG 0.473137 4.1:+36825381 None:intergenic
GCTGAGAAAGTTATGTCAAA+TGG 0.482284 4.1:-36825519 MS.gene41184:CDS
ACTGCTAGGCTTAGAGTGTA+TGG 0.483688 4.1:-36826660 MS.gene41184:CDS
TGTAACCAAAGTTATAGTTA+GGG 0.484125 4.1:+36825439 None:intergenic
TTGGAGAATTTGTATAAGCA+AGG 0.498360 4.1:-36826720 MS.gene41184:CDS
CATGATATCAATCAAGATCA+TGG 0.502262 4.1:-36825279 MS.gene41184:CDS
AAAAGAACAAATAAGTATGT+TGG 0.504717 4.1:-36826739 MS.gene41184:CDS
CATCCAACTGGCATATTGGA+AGG 0.509666 4.1:-36825384 MS.gene41184:CDS
TTACAGTAATCAAGAAACAT+TGG 0.520782 4.1:-36825421 MS.gene41184:CDS
GGTGAAGGATAAATGGGTTG+AGG 0.539516 4.1:+36826528 None:intergenic
TTAATGAGGAAGGTTCAAAT+GGG 0.550220 4.1:-36826800 MS.gene41184:CDS
AATAATGAGATTAATGAGGA+AGG 0.554945 4.1:-36826810 MS.gene41184:CDS
TTTAATAATTCTTCAGGACA+AGG 0.564633 4.1:-36825225 MS.gene41184:CDS
TTCCTTCTAAAACCTACTGC+AGG 0.572590 4.1:+36825546 None:intergenic
CTGAAACAATAGAAGACACC+TGG 0.584011 4.1:+36825349 None:intergenic
AAAGTGCTTCTACCTGCAGT+AGG 0.586289 4.1:-36825558 MS.gene41184:CDS
AATATGCCAGTTGGATGCAG+TGG 0.586423 4.1:+36825390 None:intergenic
AGAAGAAGAAGAATAATGGA+GGG 0.590346 4.1:-36826840 None:intergenic
AGAACCAGAATGTGTATCAG+TGG 0.599607 4.1:+36825311 None:intergenic
AGAGTGTATGGTCACATCGA+AGG 0.601558 4.1:-36826648 MS.gene41184:CDS
AAATATCTGCAATAGTCCAA+TGG 0.604277 4.1:-36825490 MS.gene41184:CDS
CCATTTGGACAAATAGGTGA+AGG 0.604508 4.1:+36826513 None:intergenic
GAATAATAATGAGATTAATG+AGG 0.604874 4.1:-36826814 MS.gene41184:CDS
AAATACAAGAAATCACTGCT+AGG 0.611419 4.1:-36826674 MS.gene41184:CDS
ATCCAACTGGCATATTGGAA+GGG 0.612491 4.1:-36825383 MS.gene41184:CDS
GGAAGGGAAAACAACTGACC+AGG 0.627194 4.1:-36825367 MS.gene41184:CDS
GTATTTCATCAAGTTCACGA+TGG 0.702362 4.1:-36826770 MS.gene41184:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACTAATAGTTAAAAACA+AGG + chr4.1:36825923-36825942 None:intergenic 15.0%
!! ATTATTAAAAAAGTCAAAGA+AGG + chr4.1:36826807-36826826 None:intergenic 15.0%
!! TTATTAAAAAAGTCAAAGAA+GGG + chr4.1:36826806-36826825 None:intergenic 15.0%
!!! AACTAAAAAAGCATTAAAAA+AGG - chr4.1:36826232-36826251 MS.gene41184:intron 15.0%
!!! GGTAATTGAATTATTATTTT+TGG - chr4.1:36826387-36826406 MS.gene41184:intron 15.0%
!!! TTGTTCTTATTATATTTTCA+TGG - chr4.1:36825633-36825652 MS.gene41184:intron 15.0%
!! AAAAGAACAAATAAGTATGT+TGG - chr4.1:36825304-36825323 MS.gene41184:CDS 20.0%
!! ACCAAAAATAAAATACTAGA+AGG + chr4.1:36826112-36826131 None:intergenic 20.0%
!! ATCTATATTGTGGTAAAATA+TGG - chr4.1:36825867-36825886 MS.gene41184:intron 20.0%
!! CAAAAACATTGATATGAAAA+TGG + chr4.1:36825616-36825635 None:intergenic 20.0%
!! GAATAATAATGAGATTAATG+AGG - chr4.1:36825229-36825248 MS.gene41184:CDS 20.0%
!! TCTATATTGTGGTAAAATAT+GGG - chr4.1:36825868-36825887 MS.gene41184:intron 20.0%
!! TGAAAATGAGATTTATTGAA+AGG + chr4.1:36826020-36826039 None:intergenic 20.0%
!!! ATACTTATTTGTTCTTTTGT+TGG + chr4.1:36825302-36825321 None:intergenic 20.0%
!!! ATGTTTTCATTATATACCAT+TGG + chr4.1:36826572-36826591 None:intergenic 20.0%
!!! GACTTTTTTAATAATTCTTC+AGG - chr4.1:36826812-36826831 MS.gene41184:CDS 20.0%
!!! TACTTATTTGTTCTTTTGTT+GGG + chr4.1:36825301-36825320 None:intergenic 20.0%
!!! TTCAATAAATCTCATTTTCA+TGG - chr4.1:36826020-36826039 MS.gene41184:intron 20.0%
! AAGGTTAATTTCTCCTAAAA+CGG + chr4.1:36826001-36826020 None:intergenic 25.0%
! AATAATGAGATTAATGAGGA+AGG - chr4.1:36825233-36825252 MS.gene41184:CDS 25.0%
! AATCAAGATCATGGTTATAA+TGG - chr4.1:36826773-36826792 MS.gene41184:CDS 25.0%
! ATCAAGATCATGGTTATAAT+GGG - chr4.1:36826774-36826793 MS.gene41184:CDS 25.0%
! CAAATAAATTACTCCATCTT+TGG + chr4.1:36826281-36826300 None:intergenic 25.0%
! TAAAACGGGATTTATATAGA+GGG + chr4.1:36825986-36826005 None:intergenic 25.0%
! TATAACCTAGAACTAGAAAA+AGG + chr4.1:36825573-36825592 None:intergenic 25.0%
! TATCTTTGTACGACATAAAA+TGG - chr4.1:36825763-36825782 MS.gene41184:intron 25.0%
! TGTAACCAAAGTTATAGTTA+GGG + chr4.1:36826607-36826626 None:intergenic 25.0%
! TTACAGTAATCAAGAAACAT+TGG - chr4.1:36826622-36826641 MS.gene41184:CDS 25.0%
! TTTAATAATTCTTCAGGACA+AGG - chr4.1:36826818-36826837 MS.gene41184:CDS 25.0%
!!! GCCTTCTAGTATTTTATTTT+TGG - chr4.1:36826108-36826127 MS.gene41184:intron 25.0%
!!! TTGTTGTTGTATCTTTTTCA+TGG - chr4.1:36825833-36825852 MS.gene41184:intron 25.0%
AAATACAAGAAATCACTGCT+AGG - chr4.1:36825369-36825388 MS.gene41184:CDS 30.0%
AAATATCTGCAATAGTCCAA+TGG - chr4.1:36826553-36826572 MS.gene41184:CDS 30.0%
AACAGAAACATAACCAAAGA+TGG - chr4.1:36826265-36826284 MS.gene41184:intron 30.0%
AACATACCATTTGGACAAAT+AGG + chr4.1:36825539-36825558 None:intergenic 30.0%
ACAAATAGGTGAAGGATAAA+TGG + chr4.1:36825525-36825544 None:intergenic 30.0%
AGAATCCCTAACTATAACTT+TGG - chr4.1:36826599-36826618 MS.gene41184:CDS 30.0%
AGGTTAATTTCTCCTAAAAC+GGG + chr4.1:36826000-36826019 None:intergenic 30.0%
ATTAATGAGGAAGGTTCAAA+TGG - chr4.1:36825242-36825261 MS.gene41184:CDS 30.0%
CAAATAGGTGAAGGATAAAT+GGG + chr4.1:36825524-36825543 None:intergenic 30.0%
CATGATATCAATCAAGATCA+TGG - chr4.1:36826764-36826783 MS.gene41184:CDS 30.0%
CCATATTTAAATTGTGCTAG+CGG - chr4.1:36825699-36825718 MS.gene41184:intron 30.0%
CTAAAACGGGATTTATATAG+AGG + chr4.1:36825987-36826006 None:intergenic 30.0%
CTGTAACCAAAGTTATAGTT+AGG + chr4.1:36826608-36826627 None:intergenic 30.0%
TAGCTTATTTCATGACTTTG+TGG + chr4.1:36826449-36826468 None:intergenic 30.0%
TTAATGAGGAAGGTTCAAAT+GGG - chr4.1:36825243-36825262 MS.gene41184:CDS 30.0%
TTCTGCAAACATCTATATTG+TGG - chr4.1:36825857-36825876 MS.gene41184:intron 30.0%
TTGGAGAATTTGTATAAGCA+AGG - chr4.1:36825323-36825342 MS.gene41184:CDS 30.0%
TTTCTTCATGCAAATGTGTT+TGG - chr4.1:36826170-36826189 MS.gene41184:intron 30.0%
! AAAACAAGGTGCATTTTCAA+CGG + chr4.1:36825909-36825928 None:intergenic 30.0%
! AACCTAATGAGTACTACTTT+TGG + chr4.1:36825674-36825693 None:intergenic 30.0%
! AAGGAAAGAATGTCTTTTAC+TGG - chr4.1:36825414-36825433 MS.gene41184:CDS 30.0%
! CTCTATATAAATCCCGTTTT+AGG - chr4.1:36825985-36826004 MS.gene41184:intron 30.0%
!!! TTCTTCCTTTTTCTAGTTCT+AGG - chr4.1:36825565-36825584 MS.gene41184:CDS 30.0%
ATTTCATGACTTTGTGGTTG+TGG + chr4.1:36826443-36826462 None:intergenic 35.0%
CCGCTAGCACAATTTAAATA+TGG + chr4.1:36825702-36825721 None:intergenic 35.0%
CTAGTATTCTACTGTAACGT+TGG - chr4.1:36826366-36826385 MS.gene41184:intron 35.0%
GAAGTTGAGAACATACCATT+TGG + chr4.1:36825548-36825567 None:intergenic 35.0%
GCTGAGAAAGTTATGTCAAA+TGG - chr4.1:36826524-36826543 MS.gene41184:CDS 35.0%
GTATTTCATCAAGTTCACGA+TGG - chr4.1:36825273-36825292 MS.gene41184:CDS 35.0%
TCTTGTATTTCTTCAGCACT+TGG + chr4.1:36825359-36825378 None:intergenic 35.0%
TGCCAAAAGTAGTACTCATT+AGG - chr4.1:36825669-36825688 MS.gene41184:intron 35.0%
!! GAAGCACTTTTGGATTTTGA+GGG + chr4.1:36826475-36826494 None:intergenic 35.0%
!!! AGAAGCACTTTTGGATTTTG+AGG + chr4.1:36826476-36826495 None:intergenic 35.0%
AGAACCAGAATGTGTATCAG+TGG + chr4.1:36826735-36826754 None:intergenic 40.0%
AGTTCCACTGATACACATTC+TGG - chr4.1:36826728-36826747 MS.gene41184:CDS 40.0%
ATCCAACTGGCATATTGGAA+GGG - chr4.1:36826660-36826679 MS.gene41184:CDS 40.0%
CCATTTGGACAAATAGGTGA+AGG + chr4.1:36825533-36825552 None:intergenic 40.0%
CCTTCACCTATTTGTCCAAA+TGG - chr4.1:36825530-36825549 MS.gene41184:CDS 40.0%
CTGAAACAATAGAAGACACC+TGG + chr4.1:36826697-36826716 None:intergenic 40.0%
!! TTCCTTCTAAAACCTACTGC+AGG + chr4.1:36826500-36826519 None:intergenic 40.0%
!!! TACCTGCAGTAGGTTTTAGA+AGG - chr4.1:36826495-36826514 MS.gene41184:intron 40.0%
AATATGCCAGTTGGATGCAG+TGG + chr4.1:36826656-36826675 None:intergenic 45.0%
ACTGCATCCAACTGGCATAT+TGG - chr4.1:36826655-36826674 MS.gene41184:CDS 45.0%
ACTGCTAGGCTTAGAGTGTA+TGG - chr4.1:36825383-36825402 MS.gene41184:CDS 45.0%
AGAGTGTATGGTCACATCGA+AGG - chr4.1:36825395-36825414 MS.gene41184:CDS 45.0%
CATCCAACTGGCATATTGGA+AGG - chr4.1:36826659-36826678 MS.gene41184:CDS 45.0%
TGTGGTTGTGGTACGCAATA+TGG + chr4.1:36826431-36826450 None:intergenic 45.0%
TTCCCTTCCAATATGCCAGT+TGG + chr4.1:36826665-36826684 None:intergenic 45.0%
TTGTTTCCACTGCATCCAAC+TGG - chr4.1:36826647-36826666 MS.gene41184:CDS 45.0%
! AAAGTGCTTCTACCTGCAGT+AGG - chr4.1:36826485-36826504 MS.gene41184:intron 45.0%
!! AAATTGTGCTAGCGGACTTG+TGG - chr4.1:36825707-36825726 MS.gene41184:intron 45.0%
!! GGTGAAGGATAAATGGGTTG+AGG + chr4.1:36825518-36825537 None:intergenic 45.0%
!!! ACTGCAGGTAGAAGCACTTT+TGG + chr4.1:36826485-36826504 None:intergenic 45.0%
GGAAGGGAAAACAACTGACC+AGG - chr4.1:36826676-36826695 MS.gene41184:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 36825218 36826847 36825218 ID=MS.gene41184
chr4.1 mRNA 36825218 36826847 36825218 ID=MS.gene41184.t1;Parent=MS.gene41184
chr4.1 exon 36826514 36826847 36826514 ID=MS.gene41184.t1.exon1;Parent=MS.gene41184.t1
chr4.1 CDS 36826514 36826847 36826514 ID=cds.MS.gene41184.t1;Parent=MS.gene41184.t1
chr4.1 exon 36825218 36825648 36825218 ID=MS.gene41184.t1.exon2;Parent=MS.gene41184.t1
chr4.1 CDS 36825218 36825648 36825218 ID=cds.MS.gene41184.t1;Parent=MS.gene41184.t1
Gene Sequence

>MS.gene41184

ATGGAGGGTGAGAATAATAATGAGATTAATGAGGAAGGTTCAAATGGGAATAATAGTATTTCATCAAGTTCACGATGGAACCCAACAAAAGAACAAATAAGTATGTTGGAGAATTTGTATAAGCAAGGAATAAAGACACCAAGTGCTGAAGAAATACAAGAAATCACTGCTAGGCTTAGAGTGTATGGTCACATCGAAGGAAAGAATGTCTTTTACTGGTTTCAGAATCACAAAGCTAGACAGAGACAGAAACAGAAGCAAGAAAGTATTGCTTATTTCAATCGACTTCTTCATAGACCTCAACCCATTTATCCTTCACCTATTTGTCCAAATGGTATGTTCTCAACTTCTTCCTTTTTCTAGTTCTAGGTTATAAATTTTACATATACTTTATTCCATTTTCATATCAATGTTTTTGTTCTTATTATATTTTCATGGAAAAAAATTTCTATGCCAAAAGTAGTACTCATTAGGTTTTCAGCCATATTTAAATTGTGCTAGCGGACTTGTGGTTATGCTGCAAACTTTTTAATTTTTTTTTTTTTTATCTTTGTACGACATAAAATGGTTTATAATAGAATATTATGATGTAATTTGATTCATGTAGCGAACTTGTTGTTGTTGTATCTTTTTCATGGATTCTGCAAACATCTATATTGTGGTAAAATATGGGAAGATGCAGCTAATTCCGTTGAAAATGCACCTTGTTTTTAACTATTAGTTATTTGTTACATGTTAATTCAATCTCAGTTTAGAAGACATTAGCCCTCTATATAAATCCCGTTTTAGGAGAAATTAACCTTTCAATAAATCTCATTTTCATGGAAATTAATTTCTGTAATTTTTTATTTCAAATAAGTTAATTAGTTTATTCATAATTATCTTTTAATGCCTTCTAGTATTTTATTTTTGGTATTTAGTTGATTAAATATTTCTTGATATATTTCTTGCGTTTCTTCATGCAAATGTGTTTGGTGTATGTGTTCATAAAAAAGATTAACAAATCAAGATCTTAACTAAAAAAGCATTAAAAAAGGATAACTTCAAAACAGAAACATAACCAAAGATGGAGTAATTTATTTGCTCTGTGTTTTTGTTACAAATTAAATATACTTTTTAATCTAATAATGTGTTTTTTTTTACACATTCTAGTATTCTACTGTAACGTTGGTAATTGAATTATTATTTTTGGTTTTCAGCTATCTGTGCACCATATTGCGTACCACAACCACAAAGTCATGAAATAAGCTATTACCCTCAAAATCCAAAAGTGCTTCTACCTGCAGTAGGTTTTAGAAGGAATCACGCTGAGAAAGTTATGTCAAATGGCATGTCAAATATCTGCAATAGTCCAATGGTATATAATGAAAACATGCAACAGAGAATCCCTAACTATAACTTTGGTTACAGTAATCAAGAAACATTGGATTTGTTTCCACTGCATCCAACTGGCATATTGGAAGGGAAAACAACTGACCAGGTGTCTTCTATTGTTTCAGTTTCTGCTGATAGTTCCACTGATACACATTCTGGTTCTTCTCATCATCATGATATCAATCAAGATCATGGTTATAATGGGAACAAACCCTTCTTTGACTTTTTTAATAATTCTTCAGGACAAGGTTCTTAG
Protein sequence

>MS.gene41184.t1

MEGENNNEINEEGSNGNNSISSSSRWNPTKEQISMLENLYKQGIKTPSAEEIQEITARLRVYGHIEGKNVFYWFQNHKARQRQKQKQESIAYFNRLLHRPQPIYPSPICPNAICAPYCVPQPQSHEISYYPQNPKVLLPAVGFRRNHAEKVMSNGMSNICNSPMVYNENMQQRIPNYNFGYSNQETLDLFPLHPTGILEGKTTDQVSSIVSVSADSSTDTHSGSSHHHDINQDHGYNGNKPFFDFFNNSSGQGS