Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41501.t1 | KEH34477.1 | 85.7 | 133 | 19 | 0 | 1 | 133 | 29 | 161 | 2.40E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41501.t1 | Q9FJK3 | 44.1 | 127 | 70 | 1 | 1 | 127 | 29 | 154 | 1.2e-22 | 107.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41501.t1 | A0A072UY35 | 85.7 | 133 | 19 | 0 | 1 | 133 | 29 | 161 | 1.7e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene41501.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41501.t1 | MTR_3g467080 | 85.714 | 133 | 19 | 0 | 1 | 133 | 29 | 161 | 5.87e-81 | 235 |
| MS.gene41501.t1 | MTR_4g063790 | 76.692 | 133 | 31 | 0 | 1 | 133 | 29 | 161 | 5.97e-72 | 213 |
| MS.gene41501.t1 | MTR_3g465410 | 75.940 | 133 | 32 | 0 | 1 | 133 | 1 | 133 | 2.77e-70 | 207 |
| MS.gene41501.t1 | MTR_3g466980 | 53.968 | 126 | 57 | 1 | 1 | 126 | 29 | 153 | 7.25e-43 | 140 |
| MS.gene41501.t1 | MTR_3g065100 | 47.967 | 123 | 63 | 1 | 4 | 126 | 32 | 153 | 5.02e-36 | 124 |
| MS.gene41501.t1 | MTR_3g031100 | 45.455 | 132 | 72 | 0 | 1 | 132 | 29 | 160 | 2.69e-35 | 122 |
| MS.gene41501.t1 | MTR_4g032290 | 43.077 | 130 | 74 | 0 | 4 | 133 | 32 | 161 | 2.36e-32 | 114 |
| MS.gene41501.t1 | MTR_3g031240 | 46.512 | 129 | 69 | 0 | 1 | 129 | 29 | 157 | 3.24e-32 | 112 |
| MS.gene41501.t1 | MTR_4g032260 | 40.164 | 122 | 73 | 0 | 6 | 127 | 34 | 155 | 9.76e-31 | 108 |
| MS.gene41501.t1 | MTR_4g031910 | 42.742 | 124 | 71 | 0 | 4 | 127 | 32 | 155 | 1.65e-30 | 109 |
| MS.gene41501.t1 | MTR_2g016210 | 40.909 | 132 | 78 | 0 | 1 | 132 | 29 | 160 | 2.64e-30 | 109 |
| MS.gene41501.t1 | MTR_2g035580 | 47.200 | 125 | 65 | 1 | 2 | 126 | 30 | 153 | 1.49e-29 | 105 |
| MS.gene41501.t1 | MTR_4g032620 | 38.095 | 126 | 78 | 0 | 1 | 126 | 29 | 154 | 1.79e-29 | 107 |
| MS.gene41501.t1 | MTR_5g075380 | 37.302 | 126 | 78 | 1 | 1 | 126 | 29 | 153 | 8.87e-28 | 100 |
| MS.gene41501.t1 | MTR_8g036130 | 41.406 | 128 | 73 | 1 | 1 | 126 | 29 | 156 | 4.72e-27 | 100 |
| MS.gene41501.t1 | MTR_2g035610 | 47.253 | 91 | 48 | 0 | 4 | 94 | 32 | 122 | 2.10e-26 | 95.9 |
| MS.gene41501.t1 | MTR_3g466830 | 40.000 | 125 | 74 | 1 | 1 | 125 | 28 | 151 | 6.10e-25 | 93.2 |
| MS.gene41501.t1 | MTR_3g466900 | 34.921 | 126 | 81 | 1 | 4 | 129 | 2 | 126 | 7.30e-22 | 84.7 |
| MS.gene41501.t1 | MTR_3g466890 | 35.156 | 128 | 82 | 1 | 2 | 129 | 29 | 155 | 4.65e-20 | 80.9 |
| MS.gene41501.t1 | MTR_5g047580 | 36.508 | 126 | 78 | 2 | 1 | 126 | 29 | 152 | 1.24e-19 | 79.7 |
| MS.gene41501.t1 | MTR_3g466930 | 34.127 | 126 | 82 | 1 | 4 | 129 | 2 | 126 | 2.32e-19 | 78.2 |
| MS.gene41501.t1 | MTR_1g077320 | 35.484 | 124 | 76 | 3 | 4 | 126 | 32 | 152 | 2.85e-19 | 78.6 |
| MS.gene41501.t1 | MTR_1g077390 | 37.209 | 129 | 73 | 4 | 1 | 126 | 29 | 152 | 5.66e-18 | 75.5 |
| MS.gene41501.t1 | MTR_4g028720 | 32.540 | 126 | 83 | 2 | 4 | 129 | 32 | 155 | 1.29e-17 | 74.3 |
| MS.gene41501.t1 | MTR_1g077300 | 30.769 | 130 | 86 | 2 | 4 | 133 | 32 | 157 | 1.78e-17 | 73.9 |
| MS.gene41501.t1 | MTR_7g011950 | 31.746 | 126 | 84 | 2 | 4 | 129 | 32 | 155 | 2.43e-17 | 73.9 |
| MS.gene41501.t1 | MTR_7g055800 | 42.857 | 84 | 33 | 1 | 3 | 86 | 18 | 86 | 4.50e-17 | 71.2 |
| MS.gene41501.t1 | MTR_1g084950 | 33.065 | 124 | 79 | 3 | 4 | 126 | 32 | 152 | 1.02e-16 | 72.0 |
| MS.gene41501.t1 | MTR_1g090783 | 30.159 | 126 | 86 | 2 | 1 | 126 | 28 | 151 | 8.00e-15 | 67.0 |
| MS.gene41501.t1 | MTR_7g055940 | 42.105 | 76 | 29 | 1 | 3 | 78 | 18 | 78 | 1.35e-14 | 64.7 |
| MS.gene41501.t1 | MTR_1g090697 | 29.134 | 127 | 86 | 3 | 1 | 126 | 29 | 152 | 1.58e-13 | 63.9 |
| MS.gene41501.t1 | MTR_4g084780 | 55.556 | 54 | 24 | 0 | 46 | 99 | 1 | 54 | 1.95e-12 | 60.8 |
| MS.gene41501.t1 | MTR_4g028800 | 52.381 | 42 | 20 | 0 | 1 | 42 | 27 | 68 | 5.41e-12 | 57.8 |
| MS.gene41501.t1 | MTR_1g090710 | 27.344 | 128 | 88 | 3 | 1 | 126 | 29 | 153 | 1.47e-11 | 58.5 |
| MS.gene41501.t1 | MTR_4g019670 | 35.227 | 88 | 57 | 0 | 3 | 90 | 34 | 121 | 6.75e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41501.t1 | AT5G48670 | 44.000 | 125 | 67 | 2 | 4 | 127 | 32 | 154 | 2.62e-29 | 108 |
| MS.gene41501.t1 | AT5G27810 | 52.809 | 89 | 42 | 0 | 1 | 89 | 1 | 89 | 3.93e-28 | 100 |
| MS.gene41501.t1 | AT1G65300 | 39.394 | 132 | 78 | 2 | 2 | 133 | 29 | 158 | 4.70e-24 | 94.0 |
| MS.gene41501.t1 | AT1G65330 | 36.885 | 122 | 76 | 1 | 1 | 122 | 28 | 148 | 1.99e-19 | 81.6 |
| MS.gene41501.t1 | AT3G05860 | 47.191 | 89 | 43 | 1 | 1 | 85 | 29 | 117 | 2.03e-19 | 80.5 |
| MS.gene41501.t1 | AT3G05860 | 45.161 | 93 | 47 | 1 | 1 | 89 | 29 | 121 | 3.87e-19 | 80.5 |
| MS.gene41501.t1 | AT3G05860 | 45.161 | 93 | 47 | 1 | 1 | 89 | 29 | 121 | 5.17e-19 | 80.1 |
| MS.gene41501.t1 | AT5G26630 | 32.231 | 121 | 81 | 1 | 6 | 126 | 34 | 153 | 3.18e-18 | 77.4 |
| MS.gene41501.t1 | AT5G26650 | 31.783 | 129 | 87 | 1 | 1 | 129 | 27 | 154 | 7.73e-17 | 75.5 |
| MS.gene41501.t1 | AT1G31640 | 35.766 | 137 | 73 | 5 | 2 | 132 | 29 | 156 | 8.65e-16 | 72.8 |
| MS.gene41501.t1 | AT5G27960 | 29.921 | 127 | 88 | 1 | 1 | 127 | 27 | 152 | 9.29e-15 | 69.3 |
| MS.gene41501.t1 | AT2G28700 | 32.540 | 126 | 76 | 3 | 1 | 122 | 29 | 149 | 9.14e-13 | 63.9 |
| MS.gene41501.t1 | AT1G31630 | 32.374 | 139 | 74 | 6 | 3 | 133 | 30 | 156 | 4.39e-12 | 62.0 |
| MS.gene41501.t1 | AT1G22590 | 35.802 | 81 | 52 | 0 | 1 | 81 | 29 | 109 | 8.45e-12 | 59.3 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTCATTTCCTCCTTTC+TGG | 0.057843 | 3.1:+51505664 | None:intergenic |
| ATTCATACCACAGAATTTAA+TGG | 0.196701 | 3.1:-51505626 | MS.gene41501:CDS |
| CAGGTTTGGCCATCAGATTC+AGG | 0.224706 | 3.1:-51505821 | MS.gene41501:CDS |
| TATTCTTGAGGCAAAGTATT+GGG | 0.351669 | 3.1:-51505724 | MS.gene41501:CDS |
| GTTCAAGAGTTTGCATGAAC+TGG | 0.380322 | 3.1:-51505777 | MS.gene41501:CDS |
| GATGAAATTAGCACTCTTTG+TGG | 0.382102 | 3.1:-51505890 | MS.gene41501:CDS |
| CTGAATCTGATGGCCAAACC+TGG | 0.394792 | 3.1:+51505822 | None:intergenic |
| TTTGTTTCATCAACTGCTCA+TGG | 0.410143 | 3.1:+51505696 | None:intergenic |
| AATGAAATGATGAATGATAT+TGG | 0.442815 | 3.1:+51505646 | None:intergenic |
| AGGTTTGGCCATCAGATTCA+GGG | 0.444978 | 3.1:-51505820 | MS.gene41501:CDS |
| ACAATCCTCAAGCCCAGGTT+TGG | 0.451431 | 3.1:-51505835 | MS.gene41501:CDS |
| CTCTTATTGATGAGAATTTG+AGG | 0.451804 | 3.1:-51505565 | MS.gene41501:CDS |
| GGGTTAGAAGTGTGATATCC+AGG | 0.506627 | 3.1:-51505799 | MS.gene41501:CDS |
| TCTTATTGATGAGAATTTGA+GGG | 0.508869 | 3.1:-51505564 | MS.gene41501:CDS |
| GTATTCTTGAGGCAAAGTAT+TGG | 0.524622 | 3.1:-51505725 | MS.gene41501:CDS |
| ACTCTTTGTGGAATAGAAGC+TGG | 0.543874 | 3.1:-51505878 | MS.gene41501:CDS |
| TAAATATCCATTAAATTCTG+TGG | 0.554522 | 3.1:+51505619 | None:intergenic |
| GATGGCCAAACCTGGGCTTG+AGG | 0.558655 | 3.1:+51505830 | None:intergenic |
| GCAAAACAATCCTCAAGCCC+AGG | 0.573782 | 3.1:-51505840 | MS.gene41501:CDS |
| ACAAAGGAAGGAAACCAGAA+AGG | 0.579534 | 3.1:-51505678 | MS.gene41501:CDS |
| TTCATGCAAACTCTTGAACC+TGG | 0.581655 | 3.1:+51505781 | None:intergenic |
| TGAATCTGATGGCCAAACCT+GGG | 0.592740 | 3.1:+51505823 | None:intergenic |
| CTTCTAACCCCTGAATCTGA+TGG | 0.608216 | 3.1:+51505812 | None:intergenic |
| AAGGAAGGAAACCAGAAAGG+AGG | 0.615022 | 3.1:-51505675 | MS.gene41501:CDS |
| ATGAGCAGTTGATGAAACAA+AGG | 0.621497 | 3.1:-51505694 | MS.gene41501:CDS |
| GGTTTGGCCATCAGATTCAG+GGG | 0.621539 | 3.1:-51505819 | MS.gene41501:CDS |
| GCAGTTGATGAAACAAAGGA+AGG | 0.627441 | 3.1:-51505690 | MS.gene41501:CDS |
| CAAATTCTCATCAATAAGAG+CGG | 0.638387 | 3.1:+51505568 | None:intergenic |
| TATTGATGAGAATTTGAGGG+AGG | 0.641370 | 3.1:-51505561 | MS.gene41501:CDS |
| TGGATCAAGAGGTATTCTTG+AGG | 0.709854 | 3.1:-51505736 | MS.gene41501:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAATGATGAATGATAT+TGG | + | chr3.1:51505782-51505801 | None:intergenic | 20.0% |
| !! | TAAATATCCATTAAATTCTG+TGG | + | chr3.1:51505809-51505828 | None:intergenic | 20.0% |
| ! | ATTCATACCACAGAATTTAA+TGG | - | chr3.1:51505799-51505818 | MS.gene41501:CDS | 25.0% |
| ! | TCTTATTGATGAGAATTTGA+GGG | - | chr3.1:51505861-51505880 | MS.gene41501:CDS | 25.0% |
| ACTGGAACAAAGTAAAAAGA+TGG | - | chr3.1:51505666-51505685 | MS.gene41501:CDS | 30.0% | |
| CAAATTCTCATCAATAAGAG+CGG | + | chr3.1:51505860-51505879 | None:intergenic | 30.0% | |
| CTCTTATTGATGAGAATTTG+AGG | - | chr3.1:51505860-51505879 | MS.gene41501:CDS | 30.0% | |
| ! | TATTCTTGAGGCAAAGTATT+GGG | - | chr3.1:51505701-51505720 | MS.gene41501:CDS | 30.0% |
| GATGAAATTAGCACTCTTTG+TGG | - | chr3.1:51505535-51505554 | MS.gene41501:CDS | 35.0% | |
| GGAACAAAGTAAAAAGATGG+TGG | - | chr3.1:51505669-51505688 | MS.gene41501:CDS | 35.0% | |
| GTATTCTTGAGGCAAAGTAT+TGG | - | chr3.1:51505700-51505719 | MS.gene41501:CDS | 35.0% | |
| TATTGATGAGAATTTGAGGG+AGG | - | chr3.1:51505864-51505883 | MS.gene41501:CDS | 35.0% | |
| TTTGTTTCATCAACTGCTCA+TGG | + | chr3.1:51505732-51505751 | None:intergenic | 35.0% | |
| !! | ATGAGCAGTTGATGAAACAA+AGG | - | chr3.1:51505731-51505750 | MS.gene41501:CDS | 35.0% |
| !! | TAAAAAGATGGTGGATCAAG+AGG | - | chr3.1:51505678-51505697 | MS.gene41501:CDS | 35.0% |
| ACAAAGGAAGGAAACCAGAA+AGG | - | chr3.1:51505747-51505766 | MS.gene41501:CDS | 40.0% | |
| ACTCTTTGTGGAATAGAAGC+TGG | - | chr3.1:51505547-51505566 | MS.gene41501:CDS | 40.0% | |
| ATTGGTCATTTCCTCCTTTC+TGG | + | chr3.1:51505764-51505783 | None:intergenic | 40.0% | |
| GTTCAAGAGTTTGCATGAAC+TGG | - | chr3.1:51505648-51505667 | MS.gene41501:CDS | 40.0% | |
| TTCATGCAAACTCTTGAACC+TGG | + | chr3.1:51505647-51505666 | None:intergenic | 40.0% | |
| !! | GCAGTTGATGAAACAAAGGA+AGG | - | chr3.1:51505735-51505754 | MS.gene41501:CDS | 40.0% |
| !! | TGGATCAAGAGGTATTCTTG+AGG | - | chr3.1:51505689-51505708 | MS.gene41501:CDS | 40.0% |
| AAGGAAGGAAACCAGAAAGG+AGG | - | chr3.1:51505750-51505769 | MS.gene41501:CDS | 45.0% | |
| AGGTTTGGCCATCAGATTCA+GGG | - | chr3.1:51505605-51505624 | MS.gene41501:CDS | 45.0% | |
| CTTCTAACCCCTGAATCTGA+TGG | + | chr3.1:51505616-51505635 | None:intergenic | 45.0% | |
| TGAATCTGATGGCCAAACCT+GGG | + | chr3.1:51505605-51505624 | None:intergenic | 45.0% | |
| ! | GGGTTAGAAGTGTGATATCC+AGG | - | chr3.1:51505626-51505645 | MS.gene41501:CDS | 45.0% |
| ACAATCCTCAAGCCCAGGTT+TGG | - | chr3.1:51505590-51505609 | MS.gene41501:CDS | 50.0% | |
| CAGGTTTGGCCATCAGATTC+AGG | - | chr3.1:51505604-51505623 | MS.gene41501:CDS | 50.0% | |
| CTGAATCTGATGGCCAAACC+TGG | + | chr3.1:51505606-51505625 | None:intergenic | 50.0% | |
| GCAAAACAATCCTCAAGCCC+AGG | - | chr3.1:51505585-51505604 | MS.gene41501:CDS | 50.0% | |
| GGTTTGGCCATCAGATTCAG+GGG | - | chr3.1:51505606-51505625 | MS.gene41501:CDS | 50.0% | |
| GATGGCCAAACCTGGGCTTG+AGG | + | chr3.1:51505598-51505617 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 51505523 | 51505924 | 51505523 | ID=MS.gene41501 |
| chr3.1 | mRNA | 51505523 | 51505924 | 51505523 | ID=MS.gene41501.t1;Parent=MS.gene41501 |
| chr3.1 | exon | 51505523 | 51505924 | 51505523 | ID=MS.gene41501.t1.exon1;Parent=MS.gene41501.t1 |
| chr3.1 | CDS | 51505523 | 51505924 | 51505523 | ID=cds.MS.gene41501.t1;Parent=MS.gene41501.t1 |
| Gene Sequence |
| Protein sequence |
