AlfalfaGEDB | MS.gene41591

M. sativa cultivar XinJiangDaYe / MS.gene41591

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41591.t1	RHN67787.1	85	153	23	0	1	153	3	155	6.90E-66	260

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41591.t1	Q9FJK3	34.1	126	82	1	2	127	32	156	1.2e-13	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41591.t1	G7J0M2	85.0	153	23	0	1	153	31	183	5.0e-66	260.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene41591.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41591.t1	MTR_3g031240	84.967	153	23	0	1	153	31	183	5.95e-84	244
MS.gene41591.t1	MTR_3g031100	48.148	135	70	0	3	137	33	167	1.19e-39	134
MS.gene41591.t1	MTR_3g466980	48.387	124	63	1	2	125	32	154	1.13e-34	120
MS.gene41591.t1	MTR_4g063790	43.609	133	75	0	2	134	32	164	5.95e-33	114
MS.gene41591.t1	MTR_3g465410	43.609	133	75	0	2	134	4	136	2.43e-32	112
MS.gene41591.t1	MTR_3g467080	43.411	129	73	0	2	130	32	160	1.13e-29	106
MS.gene41591.t1	MTR_2g016210	41.045	134	79	0	2	135	32	165	3.58e-29	107
MS.gene41591.t1	MTR_3g065100	38.889	126	76	1	2	127	32	156	2.50e-28	105
MS.gene41591.t1	MTR_3g466830	42.105	133	70	3	2	131	31	159	3.65e-26	97.4
MS.gene41591.t1	MTR_3g466930	40.310	129	74	2	1	128	1	127	5.30e-26	95.9
MS.gene41591.t1	MTR_3g466900	41.085	129	73	2	1	128	1	127	1.07e-25	95.1
MS.gene41591.t1	MTR_4g032620	36.090	133	85	0	2	134	32	164	1.43e-25	97.4
MS.gene41591.t1	MTR_4g032290	35.878	131	84	0	2	132	32	162	9.35e-23	90.1
MS.gene41591.t1	MTR_4g031910	35.433	127	82	0	2	128	32	158	1.89e-22	89.4
MS.gene41591.t1	MTR_3g466890	38.760	129	76	2	1	128	30	156	9.80e-22	85.9
MS.gene41591.t1	MTR_5g075380	35.772	123	78	1	2	124	32	153	3.20e-21	84.7
MS.gene41591.t1	MTR_4g032260	33.065	124	83	0	2	125	32	155	5.93e-21	84.0
MS.gene41591.t1	MTR_8g036130	36.842	133	82	1	2	132	32	164	1.16e-20	85.1
MS.gene41591.t1	MTR_2g035610	42.169	83	48	0	2	84	32	114	6.53e-20	80.1
MS.gene41591.t1	MTR_1g090710	35.433	127	81	1	2	128	32	157	1.67e-17	74.7
MS.gene41591.t1	MTR_2g035580	37.302	126	74	2	2	125	32	154	1.67e-17	74.7
MS.gene41591.t1	MTR_1g077300	35.772	123	75	2	2	124	32	150	2.37e-16	71.6
MS.gene41591.t1	MTR_1g084950	34.921	126	80	2	2	127	32	155	6.30e-16	70.9
MS.gene41591.t1	MTR_1g090783	37.692	130	73	5	2	128	31	155	1.53e-14	67.0
MS.gene41591.t1	MTR_1g077320	33.333	123	80	2	2	124	32	152	2.17e-14	66.6
MS.gene41591.t1	MTR_5g047580	34.109	129	77	3	2	127	32	155	2.74e-14	66.2
MS.gene41591.t1	MTR_7g055800	36.585	82	37	1	3	84	20	86	9.27e-14	63.2
MS.gene41591.t1	MTR_1g077390	32.520	123	81	2	2	124	32	152	1.52e-13	64.3
MS.gene41591.t1	MTR_7g011950	32.520	123	81	2	2	124	32	152	6.13e-13	62.8
MS.gene41591.t1	MTR_1g090697	29.921	127	87	2	2	128	32	156	7.91e-12	60.1
MS.gene41591.t1	MTR_4g019670	32.955	88	59	0	2	89	35	122	8.44e-12	62.0
MS.gene41591.t1	MTR_4g028720	30.894	123	83	2	2	124	32	152	2.81e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41591.t1	AT5G48670	34.328	134	83	2	2	133	32	162	2.96e-19	82.4
MS.gene41591.t1	AT5G27810	43.023	86	49	0	2	87	4	89	1.32e-18	76.6
MS.gene41591.t1	AT1G65300	38.372	86	53	0	2	87	31	116	8.20e-16	72.8
MS.gene41591.t1	AT3G05860	32.787	122	77	2	2	121	32	150	1.12e-15	71.2
MS.gene41591.t1	AT3G05860	32.520	123	78	2	2	122	32	151	2.27e-15	71.2
MS.gene41591.t1	AT3G05860	32.787	122	77	2	2	121	32	150	2.76e-15	70.9
MS.gene41591.t1	AT1G65330	29.104	134	92	2	3	135	32	163	6.27e-15	70.1
MS.gene41591.t1	AT5G26650	37.209	86	54	0	2	87	30	115	7.80e-15	70.9
MS.gene41591.t1	AT5G27960	34.884	86	56	0	2	87	30	115	9.62e-14	67.4
MS.gene41591.t1	AT5G26630	30.769	130	77	3	4	127	34	156	4.86e-13	64.3
MS.gene41591.t1	AT5G06500	31.858	113	60	3	2	112	32	129	8.71e-13	63.9
MS.gene41591.t1	AT2G28700	29.839	124	80	2	2	122	32	151	3.60e-12	63.2
MS.gene41591.t1	AT1G31640	32.558	86	57	1	2	87	31	115	7.95e-11	59.3

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACCAAAAGTTGGATTTAA+TGG	0.132212	3.1:-50087574	MS.gene41591:CDS
AATCCATTAAATCCAACTTT+TGG	0.339004	3.1:+50087571	None:intergenic
ATCAAGTCCGTAACTTCATT+AGG	0.342103	3.1:+50087618	None:intergenic
GAAGCTAGATAAAATTCATT+TGG	0.361908	3.1:+50087501	None:intergenic
GAGGTTTGGCCTTCTCCAAC+TGG	0.364214	3.1:-50087858	MS.gene41591:CDS
GAGCATCCTTTGTGGTATAG+AGG	0.426750	3.1:-50087919	MS.gene41591:CDS
ACCATCATGAAGTTGAGGTT+TGG	0.438425	3.1:-50087872	MS.gene41591:CDS
AACGAATTGAGCATCCTTTG+TGG	0.463741	3.1:-50087927	MS.gene41591:CDS
CAATTGAATCCTCTTGAGCT+TGG	0.465024	3.1:+50087547	None:intergenic
GTCCGTAACTTCATTAGGAT+TGG	0.474726	3.1:+50087623	None:intergenic
CTTGATTAATGAGAAATTGA+AGG	0.475026	3.1:-50087601	MS.gene41591:CDS
GACCAATCCTAATGAAGTTA+CGG	0.478007	3.1:-50087625	MS.gene41591:CDS
GGAAAACAAGAAGAAAGAAA+TGG	0.479640	3.1:-50087706	MS.gene41591:CDS
GAAGGAAGTCTACCAAAAGT+TGG	0.485133	3.1:-50087583	MS.gene41591:CDS
TGAAAACCATCATGAAGTTG+AGG	0.549600	3.1:-50087877	MS.gene41591:CDS
GCCAAACCTCAACTTCATGA+TGG	0.552073	3.1:+50087871	None:intergenic
ATCATCAAAATTAAATTCAG+TGG	0.561119	3.1:+50087656	None:intergenic
AGATCAGTATCACAAACTAA+AGG	0.570022	3.1:-50087730	MS.gene41591:CDS
GAGTAGTAAGTGTGTTGTCA+AGG	0.577943	3.1:-50087836	MS.gene41591:CDS
CTTACTACTCCAGTTGGAGA+AGG	0.592421	3.1:+50087849	None:intergenic
TCAGTATCACAAACTAAAGG+TGG	0.593746	3.1:-50087727	MS.gene41591:CDS
AACACACTTACTACTCCAGT+TGG	0.596457	3.1:+50087843	None:intergenic
AATGGATTTCCAAGCTCAAG+AGG	0.613698	3.1:-50087556	MS.gene41591:CDS
CACAAGCCTCTATACCACAA+AGG	0.687096	3.1:+50087913	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCATCAAAATTAAATTCAG+TGG	+	chr3.1:50087774-50087793	None:intergenic	20.0%
!	CTTGATTAATGAGAAATTGA+AGG	-	chr3.1:50087826-50087845	MS.gene41591:CDS	25.0%
!	GAAGCTAGATAAAATTCATT+TGG	+	chr3.1:50087929-50087948	None:intergenic	25.0%
!!	AATCCATTAAATCCAACTTT+TGG	+	chr3.1:50087859-50087878	None:intergenic	25.0%
	AGATCAGTATCACAAACTAA+AGG	-	chr3.1:50087697-50087716	MS.gene41591:CDS	30.0%
	CTACCAAAAGTTGGATTTAA+TGG	-	chr3.1:50087853-50087872	MS.gene41591:CDS	30.0%
	GGAAAACAAGAAGAAAGAAA+TGG	-	chr3.1:50087721-50087740	MS.gene41591:CDS	30.0%
!!!	CCTTGTTCTTTTTTCATGTT+CGG	+	chr3.1:50087630-50087649	None:intergenic	30.0%
	ATCAAGTCCGTAACTTCATT+AGG	+	chr3.1:50087812-50087831	None:intergenic	35.0%
	CCGAACATGAAAAAAGAACA+AGG	-	chr3.1:50087627-50087646	MS.gene41591:CDS	35.0%
	GACCAATCCTAATGAAGTTA+CGG	-	chr3.1:50087802-50087821	MS.gene41591:CDS	35.0%
	TCAGTATCACAAACTAAAGG+TGG	-	chr3.1:50087700-50087719	MS.gene41591:CDS	35.0%
	TGAAAAAAGAACAAGGATGC+TGG	-	chr3.1:50087634-50087653	MS.gene41591:CDS	35.0%
	TGAAAACCATCATGAAGTTG+AGG	-	chr3.1:50087550-50087569	MS.gene41591:CDS	35.0%
	AACACACTTACTACTCCAGT+TGG	+	chr3.1:50087587-50087606	None:intergenic	40.0%
	AACGAATTGAGCATCCTTTG+TGG	-	chr3.1:50087500-50087519	MS.gene41591:CDS	40.0%
	ACCATCATGAAGTTGAGGTT+TGG	-	chr3.1:50087555-50087574	MS.gene41591:CDS	40.0%
	CAATTGAATCCTCTTGAGCT+TGG	+	chr3.1:50087883-50087902	None:intergenic	40.0%
	GAAGGAAGTCTACCAAAAGT+TGG	-	chr3.1:50087844-50087863	MS.gene41591:CDS	40.0%
	GAGTAGTAAGTGTGTTGTCA+AGG	-	chr3.1:50087591-50087610	MS.gene41591:CDS	40.0%
	GTCCGTAACTTCATTAGGAT+TGG	+	chr3.1:50087807-50087826	None:intergenic	40.0%
!	AATGGATTTCCAAGCTCAAG+AGG	-	chr3.1:50087871-50087890	MS.gene41591:CDS	40.0%
	CACAAGCCTCTATACCACAA+AGG	+	chr3.1:50087517-50087536	None:intergenic	45.0%
	CTTACTACTCCAGTTGGAGA+AGG	+	chr3.1:50087581-50087600	None:intergenic	45.0%
	GAGCATCCTTTGTGGTATAG+AGG	-	chr3.1:50087508-50087527	MS.gene41591:CDS	45.0%
	GCCAAACCTCAACTTCATGA+TGG	+	chr3.1:50087559-50087578	None:intergenic	45.0%
!	GAGGTTTGGCCTTCTCCAAC+TGG	-	chr3.1:50087569-50087588	MS.gene41591:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	50087494	50087955	50087494	ID=MS.gene41591
chr3.1	mRNA	50087494	50087955	50087494	ID=MS.gene41591.t1;Parent=MS.gene41591
chr3.1	exon	50087494	50087955	50087494	ID=MS.gene41591.t1.exon1;Parent=MS.gene41591.t1
chr3.1	CDS	50087494	50087955	50087494	ID=cds.MS.gene41591.t1;Parent=MS.gene41591.t1

Gene Sequence

>MS.gene41591

ATGATCAACGAATTGAGCATCCTTTGTGGTATAGAGGCTTGTGCAATCATTTTTGATGAAAACCATCATGAAGTTGAGGTTTGGCCTTCTCCAACTGGAGTAGTAAGTGTGTTGTCAAGGTTCTCGAATTTATCCGAACATGAAAAAAGAACAAGGATGCTGGATTTAGAGAGCTACTTGAAACAAAGAATTGAGAAAGCTCAAGATCAGTATCACAAACTAAAGGTGGAAAACAAGAAGAAAGAAATGGCTGAATATATAAACAAATATATGTGTGCCACTGAATTTAATTTTGATGATGCTCACTTGACCAATCCTAATGAAGTTACGGACTTGATTAATGAGAAATTGAAGGAAGTCTACCAAAAGTTGGATTTAATGGATTTCCAAGCTCAAGAGGATTCAATTGATTTTGATGATATTGATTTCGATCCAAATGAATTTTATCTAGCTTCTCCATAA

Protein sequence

>MS.gene41591.t1

MINELSILCGIEACAIIFDENHHEVEVWPSPTGVVSVLSRFSNLSEHEKRTRMLDLESYLKQRIEKAQDQYHKLKVENKKKEMAEYINKYMCATEFNFDDAHLTNPNEVTDLINEKLKEVYQKLDLMDFQAQEDSIDFDDIDFDPNEFYLASP