Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41996.t1 | RHN49639.1 | 70.5 | 149 | 28 | 2 | 2 | 135 | 7 | 154 | 8.60E-50 | 206.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41996.t1 | A0A396H8N5 | 70.5 | 149 | 28 | 2 | 2 | 135 | 7 | 154 | 6.2e-50 | 206.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene41996.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41996.t1 | MTR_7g117590 | 68.456 | 149 | 32 | 2 | 1 | 135 | 12 | 159 | 4.98e-67 | 201 |
| MS.gene41996.t1 | MTR_1g054470 | 56.250 | 128 | 30 | 4 | 6 | 133 | 28 | 129 | 2.44e-40 | 132 |
| MS.gene41996.t1 | MTR_2g012700 | 38.144 | 97 | 59 | 1 | 40 | 135 | 11 | 107 | 3.11e-22 | 85.9 |
| MS.gene41996.t1 | MTR_2g062330 | 40.385 | 104 | 57 | 3 | 33 | 135 | 27 | 126 | 1.29e-21 | 84.7 |
| MS.gene41996.t1 | MTR_5g071400 | 70.370 | 54 | 14 | 2 | 1 | 54 | 8 | 59 | 2.79e-19 | 77.4 |
| MS.gene41996.t1 | MTR_5g071400 | 76.316 | 38 | 7 | 2 | 17 | 54 | 1 | 36 | 2.79e-12 | 58.9 |
| MS.gene41996.t1 | MTR_0010s0310 | 31.765 | 85 | 55 | 1 | 52 | 133 | 151 | 235 | 5.08e-12 | 61.6 |
| MS.gene41996.t1 | MTR_6g087720 | 34.483 | 87 | 56 | 1 | 50 | 135 | 212 | 298 | 1.69e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCGGAAATGGGTGAAAAT+TGG | 0.219124 | 6.4:-17458274 | None:intergenic |
| CCATCAACTTCTTCTTCGTT+TGG | 0.276341 | 6.4:-17458306 | None:intergenic |
| CGAATCGTTGATGATGAAAA+TGG | 0.291801 | 6.4:+17458572 | MS.gene41996:CDS |
| CTTTGGATTGCAATCTTCTC+AGG | 0.365114 | 6.4:-17458524 | None:intergenic |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 6.4:+17458605 | MS.gene41996:CDS |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 6.4:+17458612 | MS.gene41996:CDS |
| ATTGATTCACATACATACTT+TGG | 0.390585 | 6.4:-17458541 | None:intergenic |
| ACCGGAAATGGGTGAAAATT+GGG | 0.397872 | 6.4:-17458273 | None:intergenic |
| GGCTGTTGGAAGGAATGTTC+TGG | 0.414323 | 6.4:+17458404 | MS.gene41996:CDS |
| CCAAACGAAGAAGAAGTTGA+TGG | 0.464081 | 6.4:+17458306 | MS.gene41996:CDS |
| ATTAGTTCTGCTACGGCTGT+TGG | 0.466648 | 6.4:+17458390 | MS.gene41996:CDS |
| TTTGAAACTGTGCAAATACT+GGG | 0.518523 | 6.4:-17458329 | None:intergenic |
| TGAAAATGTCTTCCTCTAGC+AGG | 0.518806 | 6.4:+17457813 | None:intergenic |
| GGCATCAATTCGTCCAACAC+TGG | 0.533070 | 6.4:-17458252 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 6.4:+17458616 | MS.gene41996:CDS |
| GTTCTGCTACGGCTGTTGGA+AGG | 0.576179 | 6.4:+17458394 | MS.gene41996:CDS |
| AGGGCAAAAGAACTCAAAGC+TGG | 0.576793 | 6.4:+17458674 | MS.gene41996:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 6.4:+17458383 | MS.gene41996:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 6.4:+17458629 | MS.gene41996:CDS |
| CGATGTAATCGATCCAGTGT+TGG | 0.595779 | 6.4:+17458239 | MS.gene41996:CDS |
| ATGGAGAAATACGTTGGAAG+CGG | 0.596476 | 6.4:+17458635 | MS.gene41996:CDS |
| AGAAATACGTTGGAAGCGGA+TGG | 0.602100 | 6.4:+17458639 | MS.gene41996:CDS |
| ACTGATACAAATAATTTGTG+TGG | 0.649733 | 6.4:-17458727 | None:intergenic |
| AAATTGGAGTTACCTGCTAG+AGG | 0.649849 | 6.4:-17457825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAACAATTTTTTTTTACA+AGG | + | chr6.4:17458119-17458138 | MS.gene41996:intron | 10.0% |
| !!! | CAAAAACAATTTTTTTTTAT+AGG | - | chr6.4:17457920-17457939 | None:intergenic | 10.0% |
| !!! | CTATAAAAAAAAATTGTTTT+TGG | + | chr6.4:17457918-17457937 | MS.gene41996:intron | 10.0% |
| !!! | TTGTAAAAAAAAATTGTTTT+TGG | - | chr6.4:17458120-17458139 | None:intergenic | 10.0% |
| !! | TGAATAAATAACACAAAAAT+TGG | - | chr6.4:17457844-17457863 | None:intergenic | 15.0% |
| !!! | AAAAAACAAAAAATTTTGCA+GGG | + | chr6.4:17458093-17458112 | MS.gene41996:intron | 15.0% |
| !!! | AAAAAACAAAAATTTTTGCA+GGG | - | chr6.4:17457945-17457964 | None:intergenic | 15.0% |
| !!! | ATTTTTGTGTTATTTATTCA+AGG | + | chr6.4:17457844-17457863 | MS.gene41996:intron | 15.0% |
| !!! | TAAAAAACAAAAAATTTTGC+AGG | + | chr6.4:17458092-17458111 | MS.gene41996:intron | 15.0% |
| !!! | TAAAAAACAAAAATTTTTGC+AGG | - | chr6.4:17457946-17457965 | None:intergenic | 15.0% |
| !! | ATAACCAAATGATTGAATAA+AGG | - | chr6.4:17458194-17458213 | None:intergenic | 20.0% |
| !! | ATTGAATAAAGGCTAAAATA+TGG | - | chr6.4:17458183-17458202 | None:intergenic | 20.0% |
| !! | ATTTATTCAAGGCTAAAATA+TGG | + | chr6.4:17457855-17457874 | MS.gene41996:intron | 20.0% |
| !!! | CAAAAACAAAATATTTTGCA+GGG | + | chr6.4:17457991-17458010 | MS.gene41996:intron | 20.0% |
| !!! | CAGTTTCAAAATTTTCAAAA+TGG | + | chr6.4:17458342-17458361 | MS.gene41996:CDS | 20.0% |
| !!! | GCAATTTTATTTGTAATTTG+TGG | + | chr6.4:17458453-17458472 | MS.gene41996:intron | 20.0% |
| !!! | TCAAAAACAAAATATTTTGC+AGG | + | chr6.4:17457990-17458009 | MS.gene41996:intron | 20.0% |
| ! | ATGCAAATCATCACAAAAAT+GGG | + | chr6.4:17458055-17458074 | MS.gene41996:intron | 25.0% |
| ! | TATGCAAATCATCACAAAAA+TGG | + | chr6.4:17458054-17458073 | MS.gene41996:intron | 25.0% |
| !! | AAAATGGTGATCATACTTTT+TGG | + | chr6.4:17458358-17458377 | MS.gene41996:CDS | 25.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | - | chr6.4:17458730-17458749 | None:intergenic | 25.0% |
| !! | ATTGATTCACATACATACTT+TGG | - | chr6.4:17458544-17458563 | None:intergenic | 25.0% |
| !! | TGAACCCATTTTGTAATTTT+TGG | - | chr6.4:17458018-17458037 | None:intergenic | 25.0% |
| !! | TTAGCCTTTATTCAATCATT+TGG | + | chr6.4:17458187-17458206 | MS.gene41996:intron | 25.0% |
| !!! | TTGTTTTTTAAAATAGTCCC+TGG | + | chr6.4:17457956-17457975 | MS.gene41996:intron | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr6.4:17458612-17458631 | MS.gene41996:CDS | 30.0% | |
| AGGGACCAAAAATTACAAAA+TGG | + | chr6.4:17458010-17458029 | MS.gene41996:intron | 30.0% | |
| GGGACCAAAAATTACAAAAT+GGG | + | chr6.4:17458011-17458030 | MS.gene41996:intron | 30.0% | |
| TGCAAATCATCACAAAAATG+GGG | + | chr6.4:17458056-17458075 | MS.gene41996:intron | 30.0% | |
| ! | TTTGAAACTGTGCAAATACT+GGG | - | chr6.4:17458332-17458351 | None:intergenic | 30.0% |
| !!! | TTTTGAAACTGTGCAAATAC+TGG | - | chr6.4:17458333-17458352 | None:intergenic | 30.0% |
| !!! | TTTTTAAAATAGTCCCTGGA+GGG | + | chr6.4:17457960-17457979 | MS.gene41996:intron | 30.0% |
| !!! | TTTTTTAAAATAGTCCCTGG+AGG | + | chr6.4:17457959-17457978 | MS.gene41996:intron | 30.0% |
| AATTGGATGGAGAAATACGT+TGG | + | chr6.4:17458629-17458648 | MS.gene41996:CDS | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr6.4:17458605-17458624 | MS.gene41996:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr6.4:17458616-17458635 | MS.gene41996:CDS | 35.0% | |
| ! | GATTTTCTTCTACCGGAAAT+GGG | - | chr6.4:17458287-17458306 | None:intergenic | 35.0% |
| ! | TCGTTTGGATTTTCTTCTAC+CGG | - | chr6.4:17458294-17458313 | None:intergenic | 35.0% |
| ! | TTTGTGATGATTTGCATACG+TGG | - | chr6.4:17458052-17458071 | None:intergenic | 35.0% |
| !! | CGAATCGTTGATGATGAAAA+TGG | + | chr6.4:17458572-17458591 | MS.gene41996:CDS | 35.0% |
| !! | CGGATGGTTTGATTTTGTTA+GGG | + | chr6.4:17458655-17458674 | MS.gene41996:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr6.4:17458383-17458402 | MS.gene41996:CDS | 35.0% |
| !! | TTTTGAAAATAGTCCCTCCA+GGG | - | chr6.4:17457976-17457995 | None:intergenic | 35.0% |
| !! | TTTTTGAAAATAGTCCCTCC+AGG | - | chr6.4:17457977-17457996 | None:intergenic | 35.0% |
| AAATTGGAGTTACCTGCTAG+AGG | - | chr6.4:17457828-17457847 | None:intergenic | 40.0% | |
| ACCGGAAATGGGTGAAAATT+GGG | - | chr6.4:17458276-17458295 | None:intergenic | 40.0% | |
| ATCATCACAAAAATGGGGTC+AGG | + | chr6.4:17458061-17458080 | MS.gene41996:intron | 40.0% | |
| ATGGAGAAATACGTTGGAAG+CGG | + | chr6.4:17458635-17458654 | MS.gene41996:CDS | 40.0% | |
| CAAAACGAGGCATATTTGCA+GGG | + | chr6.4:17458150-17458169 | MS.gene41996:intron | 40.0% | |
| CAAAACGAGGCATATTTGCA+GGG | - | chr6.4:17458169-17458150 | None:intergenic | 40.0% | |
| CAAGGACTAAAACCAAAACG+AGG | + | chr6.4:17458137-17458156 | MS.gene41996:intron | 40.0% | |
| CCAAACGAAGAAGAAGTTGA+TGG | + | chr6.4:17458306-17458325 | MS.gene41996:CDS | 40.0% | |
| CCATCAACTTCTTCTTCGTT+TGG | - | chr6.4:17458309-17458328 | None:intergenic | 40.0% | |
| CTTTGGATTGCAATCTTCTC+AGG | - | chr6.4:17458527-17458546 | None:intergenic | 40.0% | |
| TACCGGAAATGGGTGAAAAT+TGG | - | chr6.4:17458277-17458296 | None:intergenic | 40.0% | |
| TAGGGACTAAAACCAAAACG+AGG | - | chr6.4:17457901-17457920 | None:intergenic | 40.0% | |
| TCATCACAAAAATGGGGTCA+GGG | + | chr6.4:17458062-17458081 | MS.gene41996:intron | 40.0% | |
| ! | GGATTTTCTTCTACCGGAAA+TGG | - | chr6.4:17458288-17458307 | None:intergenic | 40.0% |
| !! | GCGGATGGTTTGATTTTGTT+AGG | + | chr6.4:17458654-17458673 | MS.gene41996:CDS | 40.0% |
| AGAAATACGTTGGAAGCGGA+TGG | + | chr6.4:17458639-17458658 | MS.gene41996:CDS | 45.0% | |
| AGGGCAAAAGAACTCAAAGC+TGG | + | chr6.4:17458674-17458693 | MS.gene41996:CDS | 45.0% | |
| CATCGCTGCAGTTACAACAA+CGG | - | chr6.4:17458224-17458243 | None:intergenic | 45.0% | |
| CCAAAACGAGGCATATTTGC+AGG | + | chr6.4:17458149-17458168 | MS.gene41996:intron | 45.0% | |
| CCAAAACGAGGCATATTTGC+AGG | - | chr6.4:17458168-17458149 | None:intergenic | 45.0% | |
| CGATGTAATCGATCCAGTGT+TGG | + | chr6.4:17458239-17458258 | MS.gene41996:CDS | 45.0% | |
| GTTGGAAGGAATGTTCTGGT+TGG | + | chr6.4:17458408-17458427 | MS.gene41996:intron | 45.0% | |
| ! | CCTGCAAATATGCCTCGTTT+TGG | + | chr6.4:17457886-17457905 | MS.gene41996:intron | 45.0% |
| ! | CCTGCAAATATGCCTCGTTT+TGG | - | chr6.4:17457905-17457886 | None:intergenic | 45.0% |
| ! | GCCCAATTTTCACCCATTTC+CGG | + | chr6.4:17458272-17458291 | MS.gene41996:CDS | 45.0% |
| !! | ATTAGTTCTGCTACGGCTGT+TGG | + | chr6.4:17458390-17458409 | MS.gene41996:CDS | 45.0% |
| !!! | AAAAACAATTTTTTTTTATA+GGG | - | chr6.4:17457919-17457938 | None:intergenic | 5.0% |
| GGCATCAATTCGTCCAACAC+TGG | - | chr6.4:17458255-17458274 | None:intergenic | 50.0% | |
| GGCTGTTGGAAGGAATGTTC+TGG | + | chr6.4:17458404-17458423 | MS.gene41996:CDS | 50.0% | |
| GTTCTGCTACGGCTGTTGGA+AGG | + | chr6.4:17458394-17458413 | MS.gene41996:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 17457818 | 17458751 | 17457818 | ID=MS.gene41996 |
| chr6.4 | mRNA | 17457818 | 17458751 | 17457818 | ID=MS.gene41996.t1;Parent=MS.gene41996 |
| chr6.4 | exon | 17457818 | 17457834 | 17457818 | ID=MS.gene41996.t1.exon1;Parent=MS.gene41996.t1 |
| chr6.4 | CDS | 17457818 | 17457834 | 17457818 | ID=cds.MS.gene41996.t1;Parent=MS.gene41996.t1 |
| chr6.4 | exon | 17458239 | 17458425 | 17458239 | ID=MS.gene41996.t1.exon2;Parent=MS.gene41996.t1 |
| chr6.4 | CDS | 17458239 | 17458425 | 17458239 | ID=cds.MS.gene41996.t1;Parent=MS.gene41996.t1 |
| chr6.4 | exon | 17458518 | 17458751 | 17458518 | ID=MS.gene41996.t1.exon3;Parent=MS.gene41996.t1 |
| chr6.4 | CDS | 17458518 | 17458751 | 17458518 | ID=cds.MS.gene41996.t1;Parent=MS.gene41996.t1 |
| Gene Sequence |
| Protein sequence |
