AlfalfaGEDB | MS.gene42025

M. sativa cultivar XinJiangDaYe / MS.gene42025

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42025.t1	GAU15485.1	76.8	99	23	0	20	118	66	164	4.20E-34	154.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42025.t1	O64647	66.3	101	33	1	19	118	71	171	5.9e-29	128.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42025.t1	A0A1S2XYS3	73.7	99	26	0	20	118	71	169	3.3e-33	150.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene42025.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42025.t1	MTR_1g101810	73.737	99	26	0	20	118	61	159	8.00e-48	156
MS.gene42025.t1	MTR_1g101810	73.737	99	26	0	20	118	91	189	1.82e-47	156
MS.gene42025.t1	MTR_8g033070	60.748	107	35	2	17	116	74	180	6.78e-39	133
MS.gene42025.t1	MTR_7g028160	62.651	83	31	0	19	101	46	128	1.15e-31	113
MS.gene42025.t1	MTR_2g006150	52.294	109	47	1	7	110	109	217	1.14e-28	108
MS.gene42025.t1	MTR_2g006150	52.294	109	47	1	7	110	109	217	1.60e-28	107
MS.gene42025.t1	MTR_1g038650	62.025	79	29	1	7	85	17	94	2.20e-27	101
MS.gene42025.t1	MTR_1g063870	71.429	63	18	0	23	85	49	111	2.95e-27	100
MS.gene42025.t1	MTR_5g039600	58.140	86	36	0	19	104	63	148	4.66e-27	103
MS.gene42025.t1	MTR_4g108370	65.823	79	22	1	12	85	81	159	2.31e-26	101
MS.gene42025.t1	MTR_1g114380	65.672	67	23	0	19	85	29	95	3.94e-26	98.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42025.t1	AT2G45680	66.337	101	33	1	19	118	71	171	1.02e-39	135
MS.gene42025.t1	AT5G51910	70.732	82	24	0	19	100	53	134	2.16e-36	125
MS.gene42025.t1	AT5G51910	70.732	82	24	0	19	100	53	134	2.16e-36	125
MS.gene42025.t1	AT1G69690	62.963	81	30	0	5	85	34	114	4.70e-30	110
MS.gene42025.t1	AT1G58100	72.727	66	18	0	20	85	57	122	5.83e-29	108
MS.gene42025.t1	AT3G27010	71.212	66	19	0	19	84	74	139	1.60e-28	105
MS.gene42025.t1	AT3G27010	71.212	66	19	0	19	84	74	139	1.60e-28	105
MS.gene42025.t1	AT3G47620	68.657	67	21	0	19	85	113	179	7.83e-28	106
MS.gene42025.t1	AT1G35560	72.727	66	18	0	20	85	52	117	1.11e-27	103
MS.gene42025.t1	AT5G08330	67.164	67	22	0	19	85	27	93	1.04e-26	99.4
MS.gene42025.t1	AT5G23280	65.217	69	24	0	17	85	33	101	1.74e-26	99.4
MS.gene42025.t1	AT1G72010	72.727	66	18	0	20	85	60	125	2.13e-25	98.6
MS.gene42025.t1	AT2G37000	72.881	59	16	0	23	81	43	101	3.53e-25	94.4
MS.gene42025.t1	AT5G41030	60.000	70	28	0	19	88	64	133	2.42e-24	93.6
MS.gene42025.t1	AT3G45150	49.275	69	35	0	14	82	8	76	4.01e-19	78.2

Find 40 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCTTTGATGATTGC+AGG	0.311422	6.4:+16779531	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.4:+16779506	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.4:-16779565	MS.gene42025:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.4:+16779485	None:intergenic
CACCGGCACCGGAATAATTC+CGG	0.398178	6.4:-16779514	MS.gene42025:CDS
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.4:+16779601	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.4:-16779576	MS.gene42025:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.4:-16779603	MS.gene42025:CDS
GGTCTAGCTGCCGATGTTGT+TGG	0.412258	6.4:+16779456	None:intergenic
CTGTCATTTGTGGTTCCAGC+GGG	0.459106	6.4:+16779720	None:intergenic
TCGGCCGACACTGTCATTTG+TGG	0.461255	6.4:+16779710	None:intergenic
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.4:-16779481	MS.gene42025:CDS
ATCCACAAATTAACCAATTG+AGG	0.488844	6.4:+16779395	None:intergenic
ATCATCAAAGCCACCGGCAC+CGG	0.497106	6.4:-16779525	MS.gene42025:CDS
TTGCTGATAGTGTGACTCTT+CGG	0.499724	6.4:+16779643	None:intergenic
AGCTAGACCAGACGGCCAAA+AGG	0.501805	6.4:-16779442	MS.gene42025:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.4:+16779495	None:intergenic
TATCAGCAACATGTGCATCT+AGG	0.504289	6.4:-16779629	MS.gene42025:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.4:-16779408	MS.gene42025:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.4:+16779512	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.4:-16779575	MS.gene42025:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.4:+16779491	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.529649	6.4:+16779515	None:intergenic
AAAACAGACGTTGAACCCGC+TGG	0.542316	6.4:-16779735	MS.gene42025:CDS
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.4:+16779670	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.4:-16779588	MS.gene42025:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.4:-16779673	MS.gene42025:CDS
CAGACGGCCAAAAGGCCCCA+AGG	0.564342	6.4:-16779434	MS.gene42025:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.4:-16779672	MS.gene42025:CDS
AAAAGACCGTCACACGAAGG+TGG	0.615355	6.4:-16779676	MS.gene42025:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.4:-16779496	MS.gene42025:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.4:-16779492	MS.gene42025:CDS
CCTGCAATCATCAAAGCCAC+CGG	0.628378	6.4:-16779531	MS.gene42025:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.4:-16779495	MS.gene42025:CDS
ACATCGGCAGCTAGACCAGA+CGG	0.655173	6.4:-16779450	MS.gene42025:CDS
ACTGTCATTTGTGGTTCCAG+CGG	0.655351	6.4:+16779719	None:intergenic
GTTGAAGGTTCCAACAACAT+CGG	0.665250	6.4:-16779466	MS.gene42025:CDS
GGAACCACAAATGACAGTGT+CGG	0.674834	6.4:-16779714	MS.gene42025:CDS
ATCAAAAGACCGTCACACGA+AGG	0.679142	6.4:-16779679	MS.gene42025:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.4:-16779572	MS.gene42025:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GGTCTTTTGATTGTTCTTTT+CGG	+	chr6.4:16779460-16779479	None:intergenic	30.0%
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.4:16779572-16779591	MS.gene42025:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.4:16779573-16779592	MS.gene42025:CDS	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.4:16779740-16779759	MS.gene42025:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.4:16779583-16779602	MS.gene42025:CDS	40.0%
	GTTGAAGGTTCCAACAACAT+CGG	-	chr6.4:16779682-16779701	MS.gene42025:CDS	40.0%
	TATCAGCAACATGTGCATCT+AGG	-	chr6.4:16779519-16779538	MS.gene42025:CDS	40.0%
!	TTGCTGATAGTGTGACTCTT+CGG	+	chr6.4:16779508-16779527	None:intergenic	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.4:16779733-16779752	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.4:16779732-16779751	None:intergenic	40.0%
	ATCAAAAGACCGTCACACGA+AGG	-	chr6.4:16779469-16779488	MS.gene42025:CDS	45.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.4:16779576-16779595	MS.gene42025:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.4:16779560-16779579	MS.gene42025:CDS	45.0%
	GGAACCACAAATGACAGTGT+CGG	-	chr6.4:16779434-16779453	MS.gene42025:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.4:16779545-16779564	MS.gene42025:CDS	45.0%
!	ACTGTCATTTGTGGTTCCAG+CGG	+	chr6.4:16779432-16779451	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.4:16779724-16779743	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.4:16779734-16779753	None:intergenic	45.0%
	AAAACAGACGTTGAACCCGC+TGG	-	chr6.4:16779413-16779432	MS.gene42025:CDS	50.0%
	AAAAGACCGTCACACGAAGG+TGG	-	chr6.4:16779472-16779491	MS.gene42025:CDS	50.0%
	CCTGCAATCATCAAAGCCAC+CGG	-	chr6.4:16779617-16779636	MS.gene42025:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.4:16779550-16779569	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.4:16779645-16779664	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.4:16779667-16779686	MS.gene42025:CDS	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.4:16779666-16779685	None:intergenic	50.0%
!	CTGTCATTTGTGGTTCCAGC+GGG	+	chr6.4:16779431-16779450	None:intergenic	50.0%
	ACATCGGCAGCTAGACCAGA+CGG	-	chr6.4:16779698-16779717	MS.gene42025:CDS	55.0%
	AGCTAGACCAGACGGCCAAA+AGG	-	chr6.4:16779706-16779725	MS.gene42025:CDS	55.0%
	ATCATCAAAGCCACCGGCAC+CGG	-	chr6.4:16779623-16779642	MS.gene42025:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.4:16779653-16779672	MS.gene42025:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.4:16779656-16779675	MS.gene42025:CDS	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.4:16779695-16779714	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.4:16779652-16779671	MS.gene42025:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.4:16779634-16779653	MS.gene42025:CDS	55.0%
!	TCGGCCGACACTGTCATTTG+TGG	+	chr6.4:16779441-16779460	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.4:16779656-16779675	None:intergenic	55.0%
!!	CCGGTGGCTTTGATGATTGC+AGG	+	chr6.4:16779620-16779639	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.4:16779660-16779679	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.4:16779475-16779494	MS.gene42025:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.4:16779476-16779495	MS.gene42025:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.4:16779639-16779658	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.4:16779481-16779500	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.4:16779636-16779655	None:intergenic	60.0%
	CAGACGGCCAAAAGGCCCCA+AGG	-	chr6.4:16779714-16779733	MS.gene42025:CDS	65.0%
!	CTTGGGGCCTTTTGGCCGTC+TGG	+	chr6.4:16779716-16779735	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	16779404	16779766	16779404	ID=MS.gene42025
chr6.4	mRNA	16779404	16779766	16779404	ID=MS.gene42025.t1;Parent=MS.gene42025
chr6.4	exon	16779404	16779766	16779404	ID=MS.gene42025.t1.exon1;Parent=MS.gene42025.t1
chr6.4	CDS	16779404	16779766	16779404	ID=cds.MS.gene42025.t1;Parent=MS.gene42025.t1

Gene Sequence	>MS.gene42025 ATGCATGGAAAAACAGACGTTGAACCCGCTGGAACCACAAATGACAGTGTCGGCCGAAAAGAACAATCAAAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTATCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAAAGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCGGCAGCTAGACCAGACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene42025.t1 MHGKTDVEPAGTTNDSVGRKEQSKDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIIKATGTGIIPAIAISEGGKLKVPTTSAARPDGQKAPRKKSKRASIG