AlfalfaGEDB | MS.gene42030

M. sativa cultivar XinJiangDaYe / MS.gene42030

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42030.t1	XP_027366958.1	75.2	101	25	0	17	117	82	182	4.20E-34	154.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42030.t1	Q9LT89	65.4	104	35	1	3	105	37	140	1.6e-29	130.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42030.t1	A0A1S2XYS3	74.7	99	25	0	19	117	71	169	3.9e-34	153.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene42030.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42030.t1	MTR_1g101810	67.521	117	37	1	2	117	43	159	8.63e-50	161
MS.gene42030.t1	MTR_1g101810	67.521	117	37	1	2	117	73	189	2.09e-49	161
MS.gene42030.t1	MTR_8g033070	67.391	92	29	1	22	113	80	170	8.90e-38	130
MS.gene42030.t1	MTR_7g028160	58.242	91	38	0	10	100	38	128	4.62e-32	114
MS.gene42030.t1	MTR_1g038650	63.014	73	27	0	12	84	22	94	6.43e-29	105
MS.gene42030.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	1.62e-27	105
MS.gene42030.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	2.55e-27	104
MS.gene42030.t1	MTR_1g114380	60.274	73	29	0	12	84	23	95	2.94e-27	101
MS.gene42030.t1	MTR_5g039600	54.255	94	43	0	10	103	55	148	6.97e-27	102
MS.gene42030.t1	MTR_1g063870	69.841	63	19	0	22	84	49	111	9.69e-27	98.6
MS.gene42030.t1	MTR_4g108370	65.000	80	22	1	11	84	80	159	7.28e-26	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42030.t1	AT2G45680	68.041	97	30	1	22	117	75	171	3.62e-39	134
MS.gene42030.t1	AT5G51910	66.000	100	33	1	1	99	35	134	9.20e-39	132
MS.gene42030.t1	AT5G51910	66.000	100	33	1	1	99	35	134	9.20e-39	132
MS.gene42030.t1	AT1G72010	73.913	69	18	0	16	84	57	125	3.03e-31	114
MS.gene42030.t1	AT1G69690	74.603	63	16	0	22	84	52	114	3.05e-29	107
MS.gene42030.t1	AT1G35560	72.727	66	18	0	19	84	52	117	9.71e-29	106
MS.gene42030.t1	AT1G58100	71.212	66	19	0	19	84	57	122	1.08e-28	107
MS.gene42030.t1	AT5G23280	61.842	76	29	0	9	84	26	101	1.17e-28	104
MS.gene42030.t1	AT3G47620	59.091	88	31	1	2	84	92	179	1.38e-28	108
MS.gene42030.t1	AT3G27010	65.278	72	25	0	12	83	68	139	2.43e-28	105
MS.gene42030.t1	AT3G27010	65.278	72	25	0	12	83	68	139	2.43e-28	105
MS.gene42030.t1	AT5G08330	65.217	69	24	0	16	84	25	93	2.13e-27	101
MS.gene42030.t1	AT5G41030	60.870	69	27	0	19	87	65	133	4.20e-25	95.5
MS.gene42030.t1	AT2G37000	71.186	59	17	0	22	80	43	101	1.54e-24	92.4
MS.gene42030.t1	AT3G45150	54.237	59	27	0	23	81	18	76	3.69e-18	75.5

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCTTTGATGATTGC+AGG	0.311422	6.4:+16738854	None:intergenic
CACCGGCACCGGAGTAATTC+CGG	0.347482	6.4:-16738837	MS.gene42030:CDS
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.4:-16738888	MS.gene42030:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.4:+16738808	None:intergenic
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.4:+16738924	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.4:-16738899	MS.gene42030:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.4:-16738926	MS.gene42030:CDS
GGTCTAGCTGCCGATGTTGT+TGG	0.412258	6.4:+16738779	None:intergenic
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.4:-16738804	MS.gene42030:CDS
TTGCTGACAGTGTGACTCTT+CGG	0.478708	6.4:+16738966	None:intergenic
ATCATCAAAGCCACCGGCAC+CGG	0.480347	6.4:-16738848	MS.gene42030:CDS
GGCCGGAATTACTCCGGTGC+CGG	0.488436	6.4:+16738835	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.4:+16739004	None:intergenic
AGCTAGACCAGACGGCCAAA+AGG	0.501805	6.4:-16738765	MS.gene42030:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.4:+16738818	None:intergenic
TGTCAGCAACATGTGCATCT+AGG	0.504146	6.4:-16738952	MS.gene42030:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.4:-16738731	MS.gene42030:CDS
CTCCACAAATTAACCAATTG+AGG	0.513351	6.4:+16738718	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.4:-16738898	MS.gene42030:CDS
CGGAATTACTCCGGTGCCGG+TGG	0.525509	6.4:+16738838	None:intergenic
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.4:+16738814	None:intergenic
AAAACAGACGTTGAACCCAC+TGG	0.536961	6.4:-16739059	MS.gene42030:CDS
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.4:+16738993	None:intergenic
GGCTATGGCCGGAATTACTC+CGG	0.554734	6.4:+16738829	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.4:-16738911	MS.gene42030:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.4:-16738996	MS.gene42030:CDS
CAGACGGCCAAAAGGCCCCA+AGG	0.564342	6.4:-16738757	MS.gene42030:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.4:-16738995	MS.gene42030:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.4:-16738819	MS.gene42030:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.4:-16738815	MS.gene42030:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.4:-16738999	MS.gene42030:CDS
CCTGCAATCATCAAAGCCAC+CGG	0.628378	6.4:-16738854	MS.gene42030:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.4:-16738818	MS.gene42030:CDS
ACATCGGCAGCTAGACCAGA+CGG	0.655173	6.4:-16738773	MS.gene42030:CDS
GTTGAAGGTTCCAACAACAT+CGG	0.665250	6.4:-16738789	MS.gene42030:CDS
GGAACCACAAAAGACAGTGT+CGG	0.676346	6.4:-16739038	MS.gene42030:CDS
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.4:-16739002	MS.gene42030:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.4:-16738895	MS.gene42030:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.4:16738892-16738911	MS.gene42030:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.4:16738893-16738912	MS.gene42030:CDS	35.0%
!	GTCTTCTGATGGTTTCTTTT+CGG	+	chr6.4:16738779-16738798	None:intergenic	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.4:16739060-16739079	MS.gene42030:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.4:16738903-16738922	MS.gene42030:CDS	40.0%
	GTTGAAGGTTCCAACAACAT+CGG	-	chr6.4:16739002-16739021	MS.gene42030:CDS	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.4:16739053-16739072	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.4:16739052-16739071	None:intergenic	40.0%
	AAAACAGACGTTGAACCCAC+TGG	-	chr6.4:16738732-16738751	MS.gene42030:CDS	45.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.4:16738896-16738915	MS.gene42030:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.4:16738880-16738899	MS.gene42030:CDS	45.0%
	GGAACCACAAAAGACAGTGT+CGG	-	chr6.4:16738753-16738772	MS.gene42030:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.4:16738839-16738858	MS.gene42030:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.4:16738865-16738884	MS.gene42030:CDS	45.0%
	TTGCTGACAGTGTGACTCTT+CGG	+	chr6.4:16738828-16738847	None:intergenic	45.0%
!	ACTGTCTTTTGTGGTTCCAG+TGG	+	chr6.4:16738751-16738770	None:intergenic	45.0%
!	CTGTCTTTTGTGGTTCCAGT+GGG	+	chr6.4:16738750-16738769	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.4:16739044-16739063	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.4:16739054-16739073	None:intergenic	45.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.4:16738789-16738808	MS.gene42030:CDS	50.0%
	CCTGCAATCATCAAAGCCAC+CGG	-	chr6.4:16738937-16738956	MS.gene42030:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.4:16738870-16738889	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.4:16738987-16739006	MS.gene42030:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.4:16738790-16738809	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.4:16738986-16739005	None:intergenic	50.0%
	ACATCGGCAGCTAGACCAGA+CGG	-	chr6.4:16739018-16739037	MS.gene42030:CDS	55.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.4:16738792-16738811	MS.gene42030:CDS	55.0%
	AGCTAGACCAGACGGCCAAA+AGG	-	chr6.4:16739026-16739045	MS.gene42030:CDS	55.0%
	ATCATCAAAGCCACCGGCAC+CGG	-	chr6.4:16738943-16738962	MS.gene42030:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.4:16738973-16738992	MS.gene42030:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.4:16738976-16738995	MS.gene42030:CDS	55.0%
	GGCTATGGCCGGAATTACTC+CGG	+	chr6.4:16738965-16738984	None:intergenic	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.4:16739015-16739034	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.4:16738972-16738991	MS.gene42030:CDS	55.0%
!	TCGGCCGACACTGTCTTTTG+TGG	+	chr6.4:16738760-16738779	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.4:16738976-16738995	None:intergenic	55.0%
!!	CCGGTGGCTTTGATGATTGC+AGG	+	chr6.4:16738940-16738959	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.4:16738980-16738999	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.4:16738795-16738814	MS.gene42030:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.4:16738796-16738815	MS.gene42030:CDS	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.4:16738801-16738820	None:intergenic	60.0%
!	CACCGGCACCGGAGTAATTC+CGG	-	chr6.4:16738954-16738973	MS.gene42030:CDS	60.0%
	CAGACGGCCAAAAGGCCCCA+AGG	-	chr6.4:16739034-16739053	MS.gene42030:CDS	65.0%
	GGCCGGAATTACTCCGGTGC+CGG	+	chr6.4:16738959-16738978	None:intergenic	65.0%
!	CGGAATTACTCCGGTGCCGG+TGG	+	chr6.4:16738956-16738975	None:intergenic	65.0%
!	CTTGGGGCCTTTTGGCCGTC+TGG	+	chr6.4:16739036-16739055	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	16738727	16739086	16738727	ID=MS.gene42030
chr6.4	mRNA	16738727	16739086	16738727	ID=MS.gene42030.t1;Parent=MS.gene42030
chr6.4	exon	16738727	16739086	16738727	ID=MS.gene42030.t1.exon1;Parent=MS.gene42030.t1
chr6.4	CDS	16738727	16739086	16738727	ID=cds.MS.gene42030.t1;Parent=MS.gene42030.t1

Gene Sequence	>MS.gene42030 ATGGAAAAACAGACGTTGAACCCACTGGAACCACAAAAGACAGTGTCGGCCGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAAAGCCACCGGCACCGGAGTAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCGGCAGCTAGACCAGACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene42030.t1 MEKQTLNPLEPQKTVSAEKKPSEDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIIKATGTGVIPAIAISEGGKLKVPTTSAARPDGQKAPRKKSKRASIG