AlfalfaGEDB | MS.gene42032

M. sativa cultivar XinJiangDaYe / MS.gene42032

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42032.t1	XP_027366958.1	73.3	105	28	0	13	117	78	182	1.90E-34	155.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42032.t1	Q9LT89	63.5	104	37	1	3	105	37	140	2.2e-28	126.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42032.t1	A0A151U6N1	72.4	105	29	0	13	117	1	105	2.3e-34	154.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene42032.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42032.t1	MTR_1g101810	67.521	117	37	1	2	117	43	159	1.08e-49	161
MS.gene42032.t1	MTR_1g101810	67.521	117	37	1	2	117	73	189	2.80e-49	160
MS.gene42032.t1	MTR_8g033070	64.356	101	29	2	22	115	80	180	3.79e-38	131
MS.gene42032.t1	MTR_7g028160	57.143	91	39	0	10	100	38	128	8.69e-31	110
MS.gene42032.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	6.09e-30	111
MS.gene42032.t1	MTR_2g006150	58.242	91	38	0	19	109	127	217	7.47e-30	111
MS.gene42032.t1	MTR_4g108370	65.000	80	22	1	11	84	80	159	8.77e-29	108
MS.gene42032.t1	MTR_1g038650	63.014	73	27	0	12	84	22	94	2.14e-28	103
MS.gene42032.t1	MTR_5g039600	56.522	92	34	1	18	103	57	148	2.28e-28	107
MS.gene42032.t1	MTR_1g114380	59.494	79	30	1	6	84	19	95	3.11e-27	101
MS.gene42032.t1	MTR_1g063870	69.841	63	19	0	22	84	49	111	1.68e-26	98.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42032.t1	AT2G45680	67.010	97	31	1	22	117	75	171	4.35e-38	131
MS.gene42032.t1	AT5G51910	65.000	100	34	1	1	99	35	134	1.22e-37	129
MS.gene42032.t1	AT5G51910	65.000	100	34	1	1	99	35	134	1.22e-37	129
MS.gene42032.t1	AT1G72010	73.913	69	18	0	16	84	57	125	5.56e-31	113
MS.gene42032.t1	AT1G69690	74.603	63	16	0	22	84	52	114	7.17e-29	107
MS.gene42032.t1	AT1G35560	72.727	66	18	0	19	84	52	117	1.69e-28	106
MS.gene42032.t1	AT1G58100	71.212	66	19	0	19	84	57	122	1.80e-28	107
MS.gene42032.t1	AT3G47620	59.091	88	31	1	2	84	92	179	2.60e-28	107
MS.gene42032.t1	AT5G23280	61.842	76	29	0	9	84	26	101	2.93e-28	103
MS.gene42032.t1	AT3G27010	65.278	72	25	0	12	83	68	139	1.10e-27	103
MS.gene42032.t1	AT3G27010	65.278	72	25	0	12	83	68	139	1.10e-27	103
MS.gene42032.t1	AT5G08330	65.217	69	24	0	16	84	25	93	2.84e-27	100
MS.gene42032.t1	AT5G41030	62.319	69	26	0	19	87	65	133	1.75e-25	96.3
MS.gene42032.t1	AT2G37000	71.186	59	17	0	22	80	43	101	2.58e-24	92.0
MS.gene42032.t1	AT3G45150	52.542	59	28	0	23	81	18	76	1.24e-17	74.3

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCGGTGGCGTTGATGATTGC+AGG	0.349030	6.4:+16709892	None:intergenic
GGCTATGGCCGGAATTATTC+CGG	0.369975	6.4:+16709867	None:intergenic
AGAAACAATAGGGTGGTTAC+TGG	0.376057	6.4:-16709926	MS.gene42032:CDS
CACCGGCACCGGAATAATTC+CGG	0.395013	6.4:-16709875	MS.gene42032:CDS
CAACTTTCCGCCCTCTGAAA+TGG	0.397867	6.4:+16709846	None:intergenic
GACCAAGATCTCTCGTCAAC+TGG	0.398427	6.4:+16709962	None:intergenic
AAGTCTGATGGAGAAACAAT+AGG	0.400346	6.4:-16709937	MS.gene42032:CDS
TTCCAGTTGACGAGAGATCT+TGG	0.406107	6.4:-16709964	MS.gene42032:CDS
GGTCTAGCTGCCGATGTTGT+TGG	0.423161	6.4:+16709817	None:intergenic
CTGTCATTTGTGGTTCCAGC+GGG	0.459106	6.4:+16710082	None:intergenic
TCGGCCGACACTGTCATTTG+TGG	0.461255	6.4:+16710072	None:intergenic
TTCAGAGGGCGGAAAGTTGA+AGG	0.466569	6.4:-16709842	MS.gene42032:CDS
TTGCTGACAGTGTGACTCTT+CGG	0.478708	6.4:+16710004	None:intergenic
AGCTAGACCAAACGGCCAAA+AGG	0.481264	6.4:-16709803	MS.gene42032:CDS
ATCCACAAATTAACCAATTG+AGG	0.488844	6.4:+16709756	None:intergenic
TTCGTGTGACGGTCTTCTGA+TGG	0.489473	6.4:+16710042	None:intergenic
ATCATCAACGCCACCGGCAC+CGG	0.490098	6.4:-16709886	MS.gene42032:CDS
CCCTCTGAAATGGCTATGGC+CGG	0.502325	6.4:+16709856	None:intergenic
TGTCAGCAACATGTGCATCT+AGG	0.504146	6.4:-16709990	MS.gene42032:CDS
AAAAGCAAGAGAGCCTCAAT+TGG	0.505951	6.4:-16709769	MS.gene42032:CDS
GGCCGGAATTATTCCGGTGC+CGG	0.511782	6.4:+16709873	None:intergenic
AGTCTGATGGAGAAACAATA+GGG	0.517180	6.4:-16709936	MS.gene42032:CDS
TCCGCCCTCTGAAATGGCTA+TGG	0.528278	6.4:+16709852	None:intergenic
AAAACAGACGTTGAACCCGC+TGG	0.542316	6.4:-16710097	MS.gene42032:CDS
GTCCCTCCACCTTCGTGTGA+CGG	0.543321	6.4:+16710031	None:intergenic
CGGAATTATTCCGGTGCCGG+TGG	0.551061	6.4:+16709876	None:intergenic
GATCTTGGTCACAAGTCTGA+TGG	0.556205	6.4:-16709949	MS.gene42032:CDS
AGACCGTCACACGAAGGTGG+AGG	0.561466	6.4:-16710034	MS.gene42032:CDS
ACATCGGCAGCTAGACCAAA+CGG	0.561909	6.4:-16709811	MS.gene42032:CDS
CAAACGGCCAAAAGGCCCCA+AGG	0.576905	6.4:-16709795	MS.gene42032:CDS
CCTGCAATCATCAACGCCAC+CGG	0.585604	6.4:-16709892	MS.gene42032:CDS
GACCGTCACACGAAGGTGGA+GGG	0.603506	6.4:-16710033	MS.gene42032:CDS
TCCGGCCATAGCCATTTCAG+AGG	0.619818	6.4:-16709857	MS.gene42032:CDS
GCCATAGCCATTTCAGAGGG+CGG	0.621097	6.4:-16709853	MS.gene42032:CDS
AGAAGACCGTCACACGAAGG+TGG	0.622175	6.4:-16710037	MS.gene42032:CDS
CCGGCCATAGCCATTTCAGA+GGG	0.636688	6.4:-16709856	MS.gene42032:CDS
ACTGTCATTTGTGGTTCCAG+CGG	0.655351	6.4:+16710081	None:intergenic
GTTGAAGGTTCCAACAACAT+CGG	0.665250	6.4:-16709827	MS.gene42032:CDS
GGAACCACAAATGACAGTGT+CGG	0.674834	6.4:-16710076	MS.gene42032:CDS
ATCAGAAGACCGTCACACGA+AGG	0.685962	6.4:-16710040	MS.gene42032:CDS
CTGATGGAGAAACAATAGGG+TGG	0.705438	6.4:-16709933	MS.gene42032:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCTGATGGAGAAACAAT+AGG	-	chr6.4:16709930-16709949	MS.gene42032:CDS	35.0%
	AGTCTGATGGAGAAACAATA+GGG	-	chr6.4:16709931-16709950	MS.gene42032:CDS	35.0%
!	GTCTTCTGATGGTTTCTTTT+CGG	+	chr6.4:16709817-16709836	None:intergenic	35.0%
	AAAAGCAAGAGAGCCTCAAT+TGG	-	chr6.4:16710098-16710117	MS.gene42032:CDS	40.0%
	AGAAACAATAGGGTGGTTAC+TGG	-	chr6.4:16709941-16709960	MS.gene42032:CDS	40.0%
	GTTGAAGGTTCCAACAACAT+CGG	-	chr6.4:16710040-16710059	MS.gene42032:CDS	40.0%
!!	CTCTCTTGCTTTTCTTCCTT+GGG	+	chr6.4:16710091-16710110	None:intergenic	40.0%
!!	TCTCTTGCTTTTCTTCCTTG+GGG	+	chr6.4:16710090-16710109	None:intergenic	40.0%
	CTGATGGAGAAACAATAGGG+TGG	-	chr6.4:16709934-16709953	MS.gene42032:CDS	45.0%
	GATCTTGGTCACAAGTCTGA+TGG	-	chr6.4:16709918-16709937	MS.gene42032:CDS	45.0%
	GGAACCACAAATGACAGTGT+CGG	-	chr6.4:16709791-16709810	MS.gene42032:CDS	45.0%
	TGTCAGCAACATGTGCATCT+AGG	-	chr6.4:16709877-16709896	MS.gene42032:CDS	45.0%
	TTCCAGTTGACGAGAGATCT+TGG	-	chr6.4:16709903-16709922	MS.gene42032:CDS	45.0%
	TTGCTGACAGTGTGACTCTT+CGG	+	chr6.4:16709866-16709885	None:intergenic	45.0%
!	ACTGTCATTTGTGGTTCCAG+CGG	+	chr6.4:16709789-16709808	None:intergenic	45.0%
!	TTTTCTTCCTTGGGGCCTTT+TGG	+	chr6.4:16710082-16710101	None:intergenic	45.0%
!!	GCTCTCTTGCTTTTCTTCCT+TGG	+	chr6.4:16710092-16710111	None:intergenic	45.0%
	AAAACAGACGTTGAACCCGC+TGG	-	chr6.4:16709770-16709789	MS.gene42032:CDS	50.0%
	ACATCGGCAGCTAGACCAAA+CGG	-	chr6.4:16710056-16710075	MS.gene42032:CDS	50.0%
	AGCTAGACCAAACGGCCAAA+AGG	-	chr6.4:16710064-16710083	MS.gene42032:CDS	50.0%
	ATCAGAAGACCGTCACACGA+AGG	-	chr6.4:16709827-16709846	MS.gene42032:CDS	50.0%
	GACCAAGATCTCTCGTCAAC+TGG	+	chr6.4:16709908-16709927	None:intergenic	50.0%
	GGCTATGGCCGGAATTATTC+CGG	+	chr6.4:16710003-16710022	None:intergenic	50.0%
	TTCAGAGGGCGGAAAGTTGA+AGG	-	chr6.4:16710025-16710044	MS.gene42032:CDS	50.0%
	TTCGTGTGACGGTCTTCTGA+TGG	+	chr6.4:16709828-16709847	None:intergenic	50.0%
!	CAACTTTCCGCCCTCTGAAA+TGG	+	chr6.4:16710024-16710043	None:intergenic	50.0%
!	CTGTCATTTGTGGTTCCAGC+GGG	+	chr6.4:16709788-16709807	None:intergenic	50.0%
	AGAAGACCGTCACACGAAGG+TGG	-	chr6.4:16709830-16709849	MS.gene42032:CDS	55.0%
	CCGGCCATAGCCATTTCAGA+GGG	-	chr6.4:16710011-16710030	MS.gene42032:CDS	55.0%
	CCTGCAATCATCAACGCCAC+CGG	-	chr6.4:16709975-16709994	MS.gene42032:CDS	55.0%
	GCCATAGCCATTTCAGAGGG+CGG	-	chr6.4:16710014-16710033	MS.gene42032:CDS	55.0%
	GGTCTAGCTGCCGATGTTGT+TGG	+	chr6.4:16710053-16710072	None:intergenic	55.0%
	TCCGGCCATAGCCATTTCAG+AGG	-	chr6.4:16710010-16710029	MS.gene42032:CDS	55.0%
!	CACCGGCACCGGAATAATTC+CGG	-	chr6.4:16709992-16710011	MS.gene42032:CDS	55.0%
!	TCGGCCGACACTGTCATTTG+TGG	+	chr6.4:16709798-16709817	None:intergenic	55.0%
!!	CCCTCTGAAATGGCTATGGC+CGG	+	chr6.4:16710014-16710033	None:intergenic	55.0%
!!	TCCGCCCTCTGAAATGGCTA+TGG	+	chr6.4:16710018-16710037	None:intergenic	55.0%
	AGACCGTCACACGAAGGTGG+AGG	-	chr6.4:16709833-16709852	MS.gene42032:CDS	60.0%
	ATCATCAACGCCACCGGCAC+CGG	-	chr6.4:16709981-16710000	MS.gene42032:CDS	60.0%
	CAAACGGCCAAAAGGCCCCA+AGG	-	chr6.4:16710072-16710091	MS.gene42032:CDS	60.0%
	GACCGTCACACGAAGGTGGA+GGG	-	chr6.4:16709834-16709853	MS.gene42032:CDS	60.0%
	GGCCGGAATTATTCCGGTGC+CGG	+	chr6.4:16709997-16710016	None:intergenic	60.0%
	GTCCCTCCACCTTCGTGTGA+CGG	+	chr6.4:16709839-16709858	None:intergenic	60.0%
!	CGGAATTATTCCGGTGCCGG+TGG	+	chr6.4:16709994-16710013	None:intergenic	60.0%
!	CTTGGGGCCTTTTGGCCGTT+TGG	+	chr6.4:16710074-16710093	None:intergenic	60.0%
!!	CCGGTGGCGTTGATGATTGC+AGG	+	chr6.4:16709978-16709997	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	16709765	16710124	16709765	ID=MS.gene42032
chr6.4	mRNA	16709765	16710124	16709765	ID=MS.gene42032.t1;Parent=MS.gene42032
chr6.4	exon	16709765	16710124	16709765	ID=MS.gene42032.t1.exon1;Parent=MS.gene42032.t1
chr6.4	CDS	16709765	16710124	16709765	ID=cds.MS.gene42032.t1;Parent=MS.gene42032.t1

Gene Sequence	>MS.gene42032 ATGGAAAAACAGACGTTGAACCCGCTGGAACCACAAATGACAGTGTCGGCCGAAAAGAAACCATCAGAAGACCGTCACACGAAGGTGGAGGGACGTGTCCGAAGAGTCACACTGTCAGCAACATGTGCATCTAGGATCTTCCAGTTGACGAGAGATCTTGGTCACAAGTCTGATGGAGAAACAATAGGGTGGTTACTGGAACAAGCTGAACCTGCAATCATCAACGCCACCGGCACCGGAATAATTCCGGCCATAGCCATTTCAGAGGGCGGAAAGTTGAAGGTTCCAACAACATCGGCAGCTAGACCAAACGGCCAAAAGGCCCCAAGGAAGAAAAGCAAGAGAGCCTCAATTGGTTAA
Protein sequence	>MS.gene42032.t1 MEKQTLNPLEPQMTVSAEKKPSEDRHTKVEGRVRRVTLSATCASRIFQLTRDLGHKSDGETIGWLLEQAEPAIINATGTGIIPAIAISEGGKLKVPTTSAARPNGQKAPRKKSKRASIG