AlfalfaGEDB | MS.gene42033

M. sativa cultivar XinJiangDaYe / MS.gene42033

KEGG: -
GO: -
PFAM: PF03634.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42033.t1	XP_027366958.1	63.1	130	47	1	2	130	69	198	5.70E-37	163.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42033.t1	Q9LT89	59.7	119	42	2	12	124	48	166	3.2e-28	125.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42033.t1	A0A1S2XYS3	68.7	115	36	0	16	130	70	184	3.9e-35	157.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene42033.t1	TF	TCP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42033.t1	MTR_1g101810	71.074	121	35	0	10	130	54	174	9.56e-54	172
MS.gene42033.t1	MTR_1g101810	71.074	121	35	0	10	130	84	204	2.40e-53	171
MS.gene42033.t1	MTR_8g033070	56.637	113	41	3	20	124	80	192	1.13e-35	125
MS.gene42033.t1	MTR_7g028160	56.471	85	37	0	20	104	50	134	1.63e-27	102
MS.gene42033.t1	MTR_1g038650	57.143	84	36	0	3	86	15	98	3.35e-26	98.6
MS.gene42033.t1	MTR_1g114380	64.286	70	25	0	17	86	30	99	4.19e-25	95.9
MS.gene42033.t1	MTR_5g039600	50.000	98	49	0	4	101	51	148	8.69e-25	97.8
MS.gene42033.t1	MTR_2g006150	51.515	99	48	0	9	107	119	217	1.03e-24	97.8
MS.gene42033.t1	MTR_2g006150	51.515	99	48	0	9	107	119	217	1.29e-24	97.4
MS.gene42033.t1	MTR_4g108370	63.291	79	23	1	10	82	81	159	4.16e-24	95.9
MS.gene42033.t1	MTR_1g063870	60.811	74	28	1	9	82	39	111	3.26e-23	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42033.t1	AT2G45680	51.538	130	56	2	8	130	57	186	1.50e-37	130
MS.gene42033.t1	AT5G51910	58.824	119	43	2	12	124	48	166	1.01e-36	127
MS.gene42033.t1	AT5G51910	58.824	119	43	2	12	124	48	166	1.01e-36	127
MS.gene42033.t1	AT1G72010	69.118	68	21	0	15	82	58	125	4.90e-27	103
MS.gene42033.t1	AT1G69690	63.014	73	27	0	14	86	46	118	8.52e-26	99.4
MS.gene42033.t1	AT5G23280	59.494	79	30	1	4	82	25	101	9.64e-26	97.8
MS.gene42033.t1	AT5G08330	68.182	66	21	0	17	82	28	93	1.72e-25	96.7
MS.gene42033.t1	AT1G35560	67.647	68	22	0	17	84	52	119	3.47e-25	97.8
MS.gene42033.t1	AT1G58100	51.648	91	44	0	17	107	57	147	8.38e-25	97.4
MS.gene42033.t1	AT3G27010	66.154	65	22	0	17	81	75	139	3.29e-24	94.7
MS.gene42033.t1	AT3G27010	66.154	65	22	0	17	81	75	139	3.29e-24	94.7
MS.gene42033.t1	AT3G47620	63.158	76	22	1	13	82	104	179	1.92e-23	94.4
MS.gene42033.t1	AT5G41030	64.615	65	23	0	17	81	65	129	5.27e-23	90.5
MS.gene42033.t1	AT2G37000	71.930	57	16	0	20	76	43	99	1.34e-22	88.2
MS.gene42033.t1	AT3G45150	49.231	65	33	0	16	80	13	77	9.81e-17	72.4

Find 42 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTCTTGCTTCTCTTCCTT+GGG	0.135432	6.4:+16702586	None:intergenic
GCTCTCTTGCTTCTCTTCCT+TGG	0.271797	6.4:+16702585	None:intergenic
GTCAAAGGAAGGAAGGTTTA+AGG	0.276144	6.4:-16702649	MS.gene42033:CDS
AAATGGCGAATTTGTAGATT+TGG	0.320257	6.4:-16702559	MS.gene42033:CDS
GACTATGGCCGGAGTTATTC+TGG	0.327684	6.4:+16702674	None:intergenic
ATCCTCTCTGCGCATGTTGC+CGG	0.335378	6.4:+16702795	None:intergenic
CTTGGGGCATCTTGGCAGTC+TGG	0.341285	6.4:+16702603	None:intergenic
TTCTCTTCCTTGGGGCATCT+TGG	0.351064	6.4:+16702595	None:intergenic
TTCTTGCGACGGTCTTTAGA+TGG	0.354098	6.4:+16702849	None:intergenic
TCCTTCCTTTGACACGACTA+TGG	0.389640	6.4:+16702659	None:intergenic
ATCTACAAATTCGCCATTTG+CGG	0.396268	6.4:+16702563	None:intergenic
TTAGATGGTTTCTTTGCTGC+CGG	0.451557	6.4:+16702864	None:intergenic
TCCTTTGACACGACTATGGC+CGG	0.464731	6.4:+16702663	None:intergenic
GGCCGGAGTTATTCTGGTGC+CGG	0.477309	6.4:+16702680	None:intergenic
AGAAACAATAAAGTGGTTGT+TGG	0.479416	6.4:-16702733	MS.gene42033:CDS
AGAAGCAAGAGAGCCGCAAA+TGG	0.498351	6.4:-16702576	MS.gene42033:CDS
TTTCAGTTGACAAGGGAGCT+TGG	0.499064	6.4:-16702771	MS.gene42033:CDS
GGTCTAGCCGCCGATGATGT+TGG	0.499400	6.4:+16702624	None:intergenic
AGACCGTCGCAAGAAGGTGG+AGG	0.533151	6.4:-16702841	MS.gene42033:CDS
GAGCTTGGTCACAAGTCTGC+TGG	0.540791	6.4:-16702756	MS.gene42033:CDS
AGAGGATCTTTCAGTTGACA+AGG	0.553516	6.4:-16702779	MS.gene42033:CDS
CGGAGTTATTCTGGTGCCGG+TGG	0.560701	6.4:+16702683	None:intergenic
TCCGGCCATAGTCGTGTCAA+AGG	0.562881	6.4:-16702664	MS.gene42033:CDS
CCGGTGGCTTCAATGATCGC+AGG	0.563711	6.4:+16702699	None:intergenic
ATCTAAAGACCGTCGCAAGA+AGG	0.565841	6.4:-16702847	MS.gene42033:CDS
GCCATAGTCGTGTCAAAGGA+AGG	0.574007	6.4:-16702660	MS.gene42033:CDS
GTTTAAGGTTCCAACATCAT+CGG	0.575076	6.4:-16702634	MS.gene42033:CDS
TAAAGACCGTCGCAAGAAGG+TGG	0.576894	6.4:-16702844	MS.gene42033:CDS
AGATATGACAATGACAGTGC+CGG	0.584868	6.4:-16702883	MS.gene42033:CDS
CACCGGCACCAGAATAACTC+CGG	0.585207	6.4:-16702682	MS.gene42033:CDS
TCTCTTGCTTCTCTTCCTTG+GGG	0.585699	6.4:+16702587	None:intergenic
GTCCCTCCACCTTCTTGCGA+CGG	0.586255	6.4:+16702838	None:intergenic
CCTGCGATCATTGAAGCCAC+CGG	0.601370	6.4:-16702699	MS.gene42033:CDS
CTGCTGGAGAAACAATAAAG+TGG	0.601560	6.4:-16702740	MS.gene42033:CDS
GACCGTCGCAAGAAGGTGGA+GGG	0.613899	6.4:-16702840	MS.gene42033:CDS
TAGTCGTGTCAAAGGAAGGA+AGG	0.620317	6.4:-16702656	MS.gene42033:CDS
GTTGCCGGTAGTGTGACTCT+CGG	0.621625	6.4:+16702810	None:intergenic
GAGGATCTTTCAGTTGACAA+GGG	0.630963	6.4:-16702778	MS.gene42033:CDS
TGTTCCGAGAGTCACACTAC+CGG	0.631779	6.4:-16702814	MS.gene42033:CDS
TAAGGTTCCAACATCATCGG+CGG	0.648326	6.4:-16702631	MS.gene42033:CDS
CAGACTGCCAAGATGCCCCA+AGG	0.661341	6.4:-16702602	MS.gene42033:CDS
TACCGGCAACATGCGCAGAG+AGG	0.687193	6.4:-16702797	MS.gene42033:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATGGCGAATTTGTAGATT+TGG	-	chr6.4:16702877-16702896	MS.gene42033:CDS	30.0%
	AGAAACAATAAAGTGGTTGT+TGG	-	chr6.4:16702703-16702722	MS.gene42033:CDS	30.0%
	ATCTACAAATTCGCCATTTG+CGG	+	chr6.4:16702876-16702895	None:intergenic	35.0%
!	GTTTAAGGTTCCAACATCAT+CGG	-	chr6.4:16702802-16702821	MS.gene42033:CDS	35.0%
	AGAGGATCTTTCAGTTGACA+AGG	-	chr6.4:16702657-16702676	MS.gene42033:CDS	40.0%
	AGATATGACAATGACAGTGC+CGG	-	chr6.4:16702553-16702572	MS.gene42033:CDS	40.0%
	CTGCTGGAGAAACAATAAAG+TGG	-	chr6.4:16702696-16702715	MS.gene42033:CDS	40.0%
	GAGGATCTTTCAGTTGACAA+GGG	-	chr6.4:16702658-16702677	MS.gene42033:CDS	40.0%
	GTCAAAGGAAGGAAGGTTTA+AGG	-	chr6.4:16702787-16702806	MS.gene42033:CDS	40.0%
	TTAGATGGTTTCTTTGCTGC+CGG	+	chr6.4:16702575-16702594	None:intergenic	40.0%
	ATCTAAAGACCGTCGCAAGA+AGG	-	chr6.4:16702589-16702608	MS.gene42033:CDS	45.0%
	CTCTCTTGCTTCTCTTCCTT+GGG	+	chr6.4:16702853-16702872	None:intergenic	45.0%
	TAAGGTTCCAACATCATCGG+CGG	-	chr6.4:16702805-16702824	MS.gene42033:CDS	45.0%
	TAGTCGTGTCAAAGGAAGGA+AGG	-	chr6.4:16702780-16702799	MS.gene42033:CDS	45.0%
	TCCTTCCTTTGACACGACTA+TGG	+	chr6.4:16702780-16702799	None:intergenic	45.0%
	TCTCTTGCTTCTCTTCCTTG+GGG	+	chr6.4:16702852-16702871	None:intergenic	45.0%
	TTCTTGCGACGGTCTTTAGA+TGG	+	chr6.4:16702590-16702609	None:intergenic	45.0%
	TTTCAGTTGACAAGGGAGCT+TGG	-	chr6.4:16702665-16702684	MS.gene42033:CDS	45.0%
	AGAAGCAAGAGAGCCGCAAA+TGG	-	chr6.4:16702860-16702879	MS.gene42033:CDS	50.0%
	GACTATGGCCGGAGTTATTC+TGG	+	chr6.4:16702765-16702784	None:intergenic	50.0%
	GCCATAGTCGTGTCAAAGGA+AGG	-	chr6.4:16702776-16702795	MS.gene42033:CDS	50.0%
	GCTCTCTTGCTTCTCTTCCT+TGG	+	chr6.4:16702854-16702873	None:intergenic	50.0%
	TAAAGACCGTCGCAAGAAGG+TGG	-	chr6.4:16702592-16702611	MS.gene42033:CDS	50.0%
	TCCTTTGACACGACTATGGC+CGG	+	chr6.4:16702776-16702795	None:intergenic	50.0%
	TGTTCCGAGAGTCACACTAC+CGG	-	chr6.4:16702622-16702641	MS.gene42033:CDS	50.0%
!	TTCTCTTCCTTGGGGCATCT+TGG	+	chr6.4:16702844-16702863	None:intergenic	50.0%
	ATCCTCTCTGCGCATGTTGC+CGG	+	chr6.4:16702644-16702663	None:intergenic	55.0%
	CACCGGCACCAGAATAACTC+CGG	-	chr6.4:16702754-16702773	MS.gene42033:CDS	55.0%
	CCTGCGATCATTGAAGCCAC+CGG	-	chr6.4:16702737-16702756	MS.gene42033:CDS	55.0%
	GAGCTTGGTCACAAGTCTGC+TGG	-	chr6.4:16702680-16702699	MS.gene42033:CDS	55.0%
	GTTGCCGGTAGTGTGACTCT+CGG	+	chr6.4:16702629-16702648	None:intergenic	55.0%
	TCCGGCCATAGTCGTGTCAA+AGG	-	chr6.4:16702772-16702791	MS.gene42033:CDS	55.0%
	AGACCGTCGCAAGAAGGTGG+AGG	-	chr6.4:16702595-16702614	MS.gene42033:CDS	60.0%
	CAGACTGCCAAGATGCCCCA+AGG	-	chr6.4:16702834-16702853	MS.gene42033:CDS	60.0%
	CCGGTGGCTTCAATGATCGC+AGG	+	chr6.4:16702740-16702759	None:intergenic	60.0%
	GACCGTCGCAAGAAGGTGGA+GGG	-	chr6.4:16702596-16702615	MS.gene42033:CDS	60.0%
	GGCCGGAGTTATTCTGGTGC+CGG	+	chr6.4:16702759-16702778	None:intergenic	60.0%
	GGTCTAGCCGCCGATGATGT+TGG	+	chr6.4:16702815-16702834	None:intergenic	60.0%
	GTCCCTCCACCTTCTTGCGA+CGG	+	chr6.4:16702601-16702620	None:intergenic	60.0%
	TACCGGCAACATGCGCAGAG+AGG	-	chr6.4:16702639-16702658	MS.gene42033:CDS	60.0%
!	CGGAGTTATTCTGGTGCCGG+TGG	+	chr6.4:16702756-16702775	None:intergenic	60.0%
!	CTTGGGGCATCTTGGCAGTC+TGG	+	chr6.4:16702836-16702855	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	16702533	16702925	16702533	ID=MS.gene42033
chr6.4	mRNA	16702533	16702925	16702533	ID=MS.gene42033.t1;Parent=MS.gene42033
chr6.4	exon	16702533	16702925	16702533	ID=MS.gene42033.t1.exon1;Parent=MS.gene42033.t1
chr6.4	CDS	16702533	16702925	16702533	ID=cds.MS.gene42033.t1;Parent=MS.gene42033.t1

Gene Sequence

>MS.gene42033

ATGTTGAACATTGTAGAGCAAGATATGACAATGACAGTGCCGGCAGCAAAGAAACCATCTAAAGACCGTCGCAAGAAGGTGGAGGGACGTGTTCCGAGAGTCACACTACCGGCAACATGCGCAGAGAGGATCTTTCAGTTGACAAGGGAGCTTGGTCACAAGTCTGCTGGAGAAACAATAAAGTGGTTGTTGGAACAAGCTGAACCTGCGATCATTGAAGCCACCGGCACCAGAATAACTCCGGCCATAGTCGTGTCAAAGGAAGGAAGGTTTAAGGTTCCAACATCATCGGCGGCTAGACCAGACTGCCAAGATGCCCCAAGGAAGAGAAGCAAGAGAGCCGCAAATGGCGAATTTGTAGATTTGGATGATCAAGATTCAATTGCTTCCTGA

Protein sequence

>MS.gene42033.t1

MLNIVEQDMTMTVPAAKKPSKDRRKKVEGRVPRVTLPATCAERIFQLTRELGHKSAGETIKWLLEQAEPAIIEATGTRITPAIVVSKEGRFKVPTSSAARPDCQDAPRKRSKRAANGEFVDLDDQDSIAS