AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42036

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42036.t1 MTR_6g043060 70.031 327 68 2 1 297 1 327 1.34e-163 458
MS.gene42036.t1 MTR_8g083100 74.909 275 25 1 23 253 23 297 2.56e-144 415
MS.gene42036.t1 MTR_8g083100 80.952 42 8 0 258 299 416 457 4.30e-15 75.5
MS.gene42036.t1 MTR_2g100680 57.191 299 81 3 1 254 12 308 4.74e-111 329
MS.gene42036.t1 MTR_6g044340 63.281 256 70 2 68 299 8 263 2.78e-108 315
MS.gene42036.t1 MTR_2g100670 55.124 283 95 6 1 254 14 293 6.23e-95 283
MS.gene42036.t1 MTR_2g100670 55.124 283 95 6 1 254 122 401 3.21e-93 282
MS.gene42036.t1 MTR_5g029680 50.237 211 91 4 22 218 32 242 1.76e-63 208
MS.gene42036.t1 MTR_8g087540 41.438 292 148 7 15 293 5 286 4.67e-59 194
MS.gene42036.t1 MTR_6g044430 46.774 248 44 5 100 295 14 225 4.01e-55 179
MS.gene42036.t1 MTR_4g077970 44.498 209 104 3 20 216 10 218 2.48e-54 184
MS.gene42036.t1 MTR_6g044440 54.190 179 29 2 174 299 1 179 2.46e-52 170
MS.gene42036.t1 MTR_1g102860 42.601 223 111 6 7 215 20 239 9.69e-50 168
MS.gene42036.t1 MTR_7g095680 44.928 207 104 4 19 215 46 252 2.30e-49 167
MS.gene42036.t1 MTR_3g104550 43.137 204 110 3 19 217 43 245 2.69e-49 168
MS.gene42036.t1 MTR_1g061170 40.000 200 117 2 20 216 47 246 4.70e-46 161
MS.gene42036.t1 MTR_1g071360 42.079 202 105 3 22 218 32 226 1.25e-45 157
MS.gene42036.t1 MTR_1g071360 37.344 241 135 4 22 253 29 262 1.54e-45 158
MS.gene42036.t1 MTR_8g105780 38.908 293 148 11 20 293 20 300 8.80e-45 156
MS.gene42036.t1 MTR_3g101870 65.686 102 35 0 12 113 14 115 1.17e-43 153
MS.gene42036.t1 MTR_6g086805 39.720 214 102 2 20 219 9 209 2.12e-41 146
MS.gene42036.t1 MTR_5g017470 60.377 106 42 0 17 122 4 109 2.70e-41 144
MS.gene42036.t1 MTR_5g089170 67.742 93 30 0 21 113 24 116 7.45e-41 142
MS.gene42036.t1 MTR_5g010680 58.333 108 45 0 6 113 8 115 4.36e-40 143
MS.gene42036.t1 MTR_2g017890 57.407 108 46 0 12 119 5 112 1.91e-39 142
MS.gene42036.t1 MTR_6g044350 54.795 146 32 3 174 285 1 146 2.23e-39 135
MS.gene42036.t1 MTR_0020s0020 61.290 93 36 0 21 113 32 124 7.35e-37 134
MS.gene42036.t1 MTR_1g100777 58.947 95 39 0 21 115 17 111 3.46e-36 132
MS.gene42036.t1 MTR_7g091370 56.989 93 40 0 21 113 21 113 4.37e-36 129
MS.gene42036.t1 MTR_4g088900 56.701 97 38 1 21 113 32 128 5.80e-33 120
MS.gene42036.t1 MTR_4g045757 60.870 69 26 1 12 79 3 71 6.16e-21 85.1
MS.gene42036.t1 MTR_6g044510 61.972 71 19 1 237 299 18 88 1.11e-20 84.7
MS.gene42036.t1 MTR_5g047190 44.330 97 47 2 30 119 1 97 1.25e-20 85.1
MS.gene42036.t1 MTR_6g044500 48.780 82 16 3 205 261 1 81 4.33e-13 64.3
MS.gene42036.t1 MTR_1g061170 29.560 159 101 4 61 216 1 151 1.67e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42036.t1 AT4G13980 55.128 234 103 2 1 232 1 234 4.59e-84 260
MS.gene42036.t1 AT4G17750 47.685 216 92 4 22 216 52 267 2.24e-58 194
MS.gene42036.t1 AT1G32330 46.383 235 107 5 1 216 16 250 6.66e-58 193
MS.gene42036.t1 AT1G32330 46.383 235 107 5 1 216 16 250 8.23e-58 193
MS.gene42036.t1 AT5G16820 48.341 211 94 5 20 216 25 234 2.23e-57 192
MS.gene42036.t1 AT5G16820 48.341 211 94 5 20 216 25 234 2.23e-57 192
MS.gene42036.t1 AT3G02990 42.918 233 120 4 8 228 9 240 2.29e-57 191
MS.gene42036.t1 AT5G45710 37.179 312 157 8 14 295 5 307 3.08e-53 177
MS.gene42036.t1 AT5G45710 37.179 312 157 8 14 295 5 307 3.08e-53 177
MS.gene42036.t1 AT4G18880 47.393 211 101 3 16 220 9 215 3.93e-51 173
MS.gene42036.t1 AT2G26150 42.342 222 120 4 19 232 41 262 5.65e-50 169
MS.gene42036.t1 AT2G26150 42.342 222 120 4 19 232 41 262 5.65e-50 169
MS.gene42036.t1 AT3G22830 39.815 216 117 3 19 221 58 273 2.34e-46 161
MS.gene42036.t1 AT3G51910 41.232 211 107 5 22 226 29 228 3.23e-46 157
MS.gene42036.t1 AT5G03720 41.584 202 110 3 20 213 53 254 2.55e-45 158
MS.gene42036.t1 AT5G03720 41.584 202 110 3 20 213 53 254 2.55e-45 158
MS.gene42036.t1 AT3G24520 45.665 173 74 3 20 191 15 168 4.31e-44 153
MS.gene42036.t1 AT1G67970 44.792 192 93 5 20 201 17 205 9.60e-44 153
MS.gene42036.t1 AT1G46264 61.607 112 33 1 21 122 33 144 3.44e-43 151
MS.gene42036.t1 AT5G43840 41.711 187 97 3 21 205 18 194 1.73e-41 145
MS.gene42036.t1 AT5G54070 42.623 183 99 2 20 201 69 246 3.93e-40 143
MS.gene42036.t1 AT3G63350 31.295 278 164 7 19 295 25 276 1.52e-38 137
MS.gene42036.t1 AT4G11660 60.000 100 40 0 14 113 51 150 1.82e-37 137
MS.gene42036.t1 AT4G36990 56.481 108 45 1 8 115 2 107 4.52e-37 134
MS.gene42036.t1 AT5G62020 49.580 119 59 1 1 118 1 119 1.26e-36 133
MS.gene42036.t1 AT2G41690 50.435 115 57 0 22 136 40 154 1.82e-35 128
MS.gene42036.t1 AT4G18870 45.455 88 48 0 22 109 146 233 1.09e-21 92.8
MS.gene42036.t1 AT4G18870 44.792 96 52 1 14 109 5 99 2.19e-21 92.0
MS.gene42036.t1 AT1G77570 41.304 92 50 3 19 106 22 113 1.22e-15 73.2
MS.gene42036.t1 AT2G26150 32.075 159 100 4 82 232 49 207 2.64e-15 75.1
MS.gene42036.t1 AT2G26150 32.075 159 100 4 82 232 49 207 2.64e-15 75.1
MS.gene42036.t1 AT5G45710 28.182 220 119 9 106 295 1 211 1.39e-12 66.6
MS.gene42036.t1 AT5G45710 28.182 220 119 9 106 295 1 211 1.39e-12 66.6
MS.gene42036.t1 AT4G19630 32.990 97 62 1 19 112 8 104 3.54e-12 63.2

Find 72 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCAATGCTGCCCTTTC+TGG -0.010683 6.4:+16658839 None:intergenic
CTTCTTTGAGAAGGCTCTTC+AGG 0.211830 6.4:-16658672 MS.gene42036:CDS
ATAATTATAGCTTAAGAGTT+TGG 0.284728 6.4:+16658775 None:intergenic
GAAATTGCTGTGTTTGAAAT+AGG 0.299469 6.4:+16660045 None:intergenic
CCACAGATGAGATCGTTTCT+TGG 0.319260 6.4:-16660132 MS.gene42036:CDS
CAAAATCGAATCCATGGATT+TGG 0.330894 6.4:-16658621 MS.gene42036:CDS
AAGGAGTGAATGATGTGTTT+TGG 0.360172 6.4:-16658403 MS.gene42036:CDS
ATAACAAAAGCTTCATCGTT+TGG 0.362747 6.4:-16660102 MS.gene42036:CDS
GTCTGAACAGCATGGACTTT+CGG 0.368229 6.4:+16658367 None:intergenic
AGAAGAACGCGAGAGAATTC+AGG 0.373908 6.4:+16660073 None:intergenic
AAAATCAGATCCTGATCGTT+GGG 0.392684 6.4:-16658969 MS.gene42036:CDS
ACGTTACCTGAAGCCTCCTT+TGG 0.398346 6.4:+16658449 None:intergenic
GAAGCCTCCTTTGGATCAAC+GGG 0.411360 6.4:+16658458 None:intergenic
TAAGAAAAGGCGATTGTCTC+AGG 0.414889 6.4:-16658588 MS.gene42036:CDS
ACCAAGCTACCTTCGGCAAC+AGG 0.417714 6.4:+16658551 None:intergenic
AGCTTAAGAGTTTGGATTGA+AGG 0.419735 6.4:+16658783 None:intergenic
TGGAAACTTATAATAAGAAA+AGG 0.461564 6.4:-16658601 MS.gene42036:CDS
GCCGAGCAGCTGCTTCGACT+TGG 0.465666 6.4:+16658426 None:intergenic
TCTGCTTCCGTCGGCAGCAG+TGG 0.466428 6.4:+16660207 None:intergenic
CCACAGTCATAGTCATCCTC+CGG 0.469987 6.4:-16658876 MS.gene42036:CDS
TTCCCGCAAAATCGAATCCA+TGG 0.478636 6.4:-16658627 MS.gene42036:CDS
TTGCTGTGTTTGAAATAGGC+TGG 0.480359 6.4:+16660049 None:intergenic
GCTTAAGAGTTTGGATTGAA+GGG 0.480417 6.4:+16658784 None:intergenic
ATTATAAGTTTCCAAATCCA+TGG 0.481800 6.4:+16658610 None:intergenic
GTTGATCCAAAGGAGGCTTC+AGG 0.485386 6.4:-16658455 MS.gene42036:CDS
AGATCCAGAAAGGGCAGCAT+TGG 0.494908 6.4:-16658843 MS.gene42036:CDS
CGACGGAAGCAGAGAAGGTA+TGG 0.499479 6.4:-16660197 MS.gene42036:CDS
TGAGACAGAAAAGCTTCAGC+TGG 0.500611 6.4:-16658750 MS.gene42036:CDS
ATTGAGCTGACGGATGAAGC+TGG 0.506271 6.4:+16660021 None:intergenic
TTTGCAGAACTTCTTTGAGA+AGG 0.509914 6.4:-16658681 MS.gene42036:CDS
GCCTGTTGCCGAAGGTAGCT+TGG 0.510527 6.4:-16658552 MS.gene42036:CDS
TTCTCTCAGAATCTTTCTGA+AGG 0.511282 6.4:-16658512 MS.gene42036:CDS
AACTGACCCCGTTGATCCAA+AGG 0.528185 6.4:-16658465 MS.gene42036:CDS
CTTTCTGAAGGAGAACTGAC+AGG 0.534056 6.4:-16658500 MS.gene42036:CDS
GAAAATCAGATCCTGATCGT+TGG 0.539910 6.4:-16658970 MS.gene42036:CDS
GTCGAAGCAGCTGCTCGGCA+AGG 0.540320 6.4:-16658422 MS.gene42036:CDS
AAGGTCAACCAAGCTACCTT+CGG 0.541708 6.4:+16658544 None:intergenic
TCTGAGAGAAATTGCTAGGA+AGG 0.542451 6.4:+16658525 None:intergenic
TCATACGTCTTCTGAAGAAA+CGG 0.543831 6.4:+16660172 None:intergenic
TTCTACTGTAGATCCAGAAA+GGG 0.545124 6.4:-16658852 MS.gene42036:CDS
TCCAAGTCGAAGCAGCTGCT+CGG 0.548536 6.4:-16658427 MS.gene42036:CDS
TGAAGCCTCCTTTGGATCAA+CGG 0.548927 6.4:+16658457 None:intergenic
TCAGCGGCGATTGGAAGGCA+TGG 0.549084 6.4:-16658720 MS.gene42036:CDS
CCGGAGGATGACTATGACTG+TGG 0.551542 6.4:+16658876 None:intergenic
CACAGTCATAGTCATCCTCC+GGG 0.559092 6.4:-16658875 MS.gene42036:CDS
GTTCTACTGTAGATCCAGAA+AGG 0.564434 6.4:-16658853 MS.gene42036:CDS
AGATTCTGAGAGAAATTGCT+AGG 0.568077 6.4:+16658521 None:intergenic
GACTGTGGATAGGTTTCTTG+CGG 0.574041 6.4:+16658891 None:intergenic
TGCTGTGTTTGAAATAGGCT+GGG 0.577118 6.4:+16660050 None:intergenic
GCTGCCGACGGAAGCAGAGA+AGG 0.581055 6.4:-16660202 MS.gene42036:CDS
AGAAACGATCTCATCTGTGG+TGG 0.582151 6.4:+16660135 None:intergenic
AACAGCATGGACTTTCGGTA+AGG 0.583587 6.4:+16658372 None:intergenic
GATTGGAAGGCATGGAGAAG+AGG 0.593047 6.4:-16658712 MS.gene42036:CDS
CACATGCAGCCTGTTGCCGA+AGG 0.596050 6.4:-16658560 MS.gene42036:CDS
TCTTCATTGTCTGAACAGCA+TGG 0.597736 6.4:+16658359 None:intergenic
TTACATAGGTATTGAGCTGA+CGG 0.598207 6.4:+16660011 None:intergenic
TCATCAGCCACTGCTGCCGA+CGG 0.606067 6.4:-16660214 MS.gene42036:CDS
GGATGACTATGACTGTGGAT+AGG 0.617132 6.4:+16658881 None:intergenic
CATACCTTCTCTGCTTCCGT+CGG 0.623107 6.4:+16660198 None:intergenic
GAAACGATCTCATCTGTGGT+GGG 0.626600 6.4:+16660136 None:intergenic
AGAACGCGAGAGAATTCAGG+AGG 0.628084 6.4:+16660076 None:intergenic
AAATCAGATCCTGATCGTTG+GGG 0.629660 6.4:-16658968 MS.gene42036:CDS
CCAAGAAACGATCTCATCTG+TGG 0.633047 6.4:+16660132 None:intergenic
ATGCTGTTCAGACAATGAAG+AGG 0.637659 6.4:-16658357 MS.gene42036:CDS
GAACGCGAGAGAATTCAGGA+GGG 0.662504 6.4:+16660077 None:intergenic
TCTTCAGAAGACGTATGACA+TGG 0.665030 6.4:-16660167 MS.gene42036:CDS
AATCAGATCCTGATCGTTGG+GGG 0.675343 6.4:-16658967 MS.gene42036:CDS
TCTGGATCTACAGTAGAACC+CGG 0.676402 6.4:+16658857 None:intergenic
TGACCCCGTTGATCCAAAGG+AGG 0.688995 6.4:-16658462 MS.gene42036:CDS
GCAGTGGCTGATGATGATTG+TGG 0.706595 6.4:+16660223 None:intergenic
GGATCTACAGTAGAACCCGG+AGG 0.764137 6.4:+16658860 None:intergenic
AAGCCTCCTTTGGATCAACG+GGG 0.785647 6.4:+16658459 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTATAAATTTTTCAT+TGG + chr6.4:16659641-16659660 None:intergenic 10.0%
!! TTATCATATTTATATAGTTC+AGG + chr6.4:16659252-16659271 None:intergenic 15.0%
!!! ATGTATGTTTTAATTTGTTA+CGG - chr6.4:16659553-16659572 MS.gene42036:intron 15.0%
!! AGGATTAAACAATAAAAATC+AGG + chr6.4:16659281-16659300 None:intergenic 20.0%
!! ATAATTATAGCTTAAGAGTT+TGG + chr6.4:16659806-16659825 None:intergenic 20.0%
!! ATATTCTTGTAGAGATTTAA+TGG - chr6.4:16659478-16659497 MS.gene42036:intron 20.0%
!! ATTATTTCATTGAGTTTAGT+TGG - chr6.4:16658828-16658847 MS.gene42036:CDS 20.0%
!! CTTTATTAAACAAAAACTTC+AGG + chr6.4:16659457-16659476 None:intergenic 20.0%
!! TGGAAACTTATAATAAGAAA+AGG - chr6.4:16659977-16659996 MS.gene42036:intron 20.0%
!!! TTTGTTGTATTTGTATGATT+TGG - chr6.4:16658682-16658701 MS.gene42036:CDS 20.0%
! AAATAGAAATCATGCACTTA+TGG - chr6.4:16659393-16659412 MS.gene42036:intron 25.0%
! AATGGTAAATCGTTCAAAAT+AGG - chr6.4:16659496-16659515 MS.gene42036:intron 25.0%
! ATATTCAGAGACTAAATTGA+TGG + chr6.4:16659208-16659227 None:intergenic 25.0%
! ATTAGGTACAACTTTAGATT+TGG - chr6.4:16658805-16658824 MS.gene42036:CDS 25.0%
! ATTATAAGTTTCCAAATCCA+TGG + chr6.4:16659971-16659990 None:intergenic 25.0%
! TGATATCTAAGTCTTAGATA+GGG - chr6.4:16659365-16659384 MS.gene42036:intron 25.0%
! TGATTAGCAAGATAAGAATT+AGG - chr6.4:16658788-16658807 MS.gene42036:CDS 25.0%
! TTCAGTATAATCATATGTGT+CGG - chr6.4:16658994-16659013 MS.gene42036:intron 25.0%
! TTGATATCTAAGTCTTAGAT+AGG - chr6.4:16659364-16659383 MS.gene42036:intron 25.0%
!!! TGAGATATTATAGTCTTGTA+TGG - chr6.4:16658891-16658910 MS.gene42036:CDS 25.0%
AAAAAAATTTCTGCAGCATC+TGG - chr6.4:16659145-16659164 MS.gene42036:intron 30.0%
AGATCAAAAACATCTTCTGA+CGG - chr6.4:16659657-16659676 MS.gene42036:intron 30.0%
ATAACAAAAGCTTCATCGTT+TGG - chr6.4:16658476-16658495 MS.gene42036:CDS 30.0%
ATATAGTTCAGGAACTAAAC+CGG + chr6.4:16659241-16659260 None:intergenic 30.0%
ATCGTTCAAAATAGGTCTTA+TGG - chr6.4:16659504-16659523 MS.gene42036:intron 30.0%
CTTCATCGAATTTCAATTAG+TGG - chr6.4:16658716-16658735 MS.gene42036:CDS 30.0%
TCTGAATATCACACTATTTC+CGG - chr6.4:16659219-16659238 MS.gene42036:intron 30.0%
! GAAATTGCTGTGTTTGAAAT+AGG + chr6.4:16658536-16658555 None:intergenic 30.0%
!! TTTACGTGTGCTTTTTATCA+GGG - chr6.4:16659580-16659599 MS.gene42036:intron 30.0%
AAAACTTCAGGAAGCAAATC+TGG + chr6.4:16659445-16659464 None:intergenic 35.0%
AAAATCAGATCCTGATCGTT+GGG - chr6.4:16659609-16659628 MS.gene42036:intron 35.0%
AAATTAAGACCTACGAAGCA+AGG + chr6.4:16659062-16659081 None:intergenic 35.0%
AGCTTAAGAGTTTGGATTGA+AGG + chr6.4:16659798-16659817 None:intergenic 35.0%
CAAAATCGAATCCATGGATT+TGG - chr6.4:16659957-16659976 MS.gene42036:intron 35.0%
GACATGAGAATGTTGAAAGA+TGG - chr6.4:16658867-16658886 MS.gene42036:CDS 35.0%
GCTTAAGAGTTTGGATTGAA+GGG + chr6.4:16659797-16659816 None:intergenic 35.0%
TTCTACTGTAGATCCAGAAA+GGG - chr6.4:16659726-16659745 MS.gene42036:intron 35.0%
TTCTCTCAGAATCTTTCTGA+AGG - chr6.4:16660066-16660085 MS.gene42036:CDS 35.0%
! AAGGAGTGAATGATGTGTTT+TGG - chr6.4:16660175-16660194 MS.gene42036:CDS 35.0%
! AATCCATGGATTCGATTTTG+CGG + chr6.4:16659957-16659976 None:intergenic 35.0%
! AGATTCTGAGAGAAATTGCT+AGG + chr6.4:16660060-16660079 None:intergenic 35.0%
! AGGAGTGAATGATGTGTTTT+GGG - chr6.4:16660176-16660195 MS.gene42036:CDS 35.0%
! GCTTCGTAGGTCTTAATTTT+CGG - chr6.4:16659063-16659082 MS.gene42036:intron 35.0%
! TAGCGTGAATTGACTTACAT+AGG + chr6.4:16658584-16658603 None:intergenic 35.0%
! TCATACGTCTTCTGAAGAAA+CGG + chr6.4:16658409-16658428 None:intergenic 35.0%
! TTTGCAGAACTTCTTTGAGA+AGG - chr6.4:16659897-16659916 MS.gene42036:intron 35.0%
!! GTTTACGTGTGCTTTTTATC+AGG - chr6.4:16659579-16659598 MS.gene42036:intron 35.0%
!! TAGGTCTTAATTTTCGGTGT+GGG - chr6.4:16659069-16659088 MS.gene42036:intron 35.0%
!! TTACATAGGTATTGAGCTGA+CGG + chr6.4:16658570-16658589 None:intergenic 35.0%
AAATCAGATCCTGATCGTTG+GGG - chr6.4:16659610-16659629 MS.gene42036:intron 40.0%
ATGCTGTTCAGACAATGAAG+AGG - chr6.4:16660221-16660240 MS.gene42036:CDS 40.0%
GAAAATCAGATCCTGATCGT+TGG - chr6.4:16659608-16659627 MS.gene42036:intron 40.0%
GTTCTACTGTAGATCCAGAA+AGG - chr6.4:16659725-16659744 MS.gene42036:intron 40.0%
TAAGAAAAGGCGATTGTCTC+AGG - chr6.4:16659990-16660009 MS.gene42036:intron 40.0%
TCTGAGAGAAATTGCTAGGA+AGG + chr6.4:16660056-16660075 None:intergenic 40.0%
TCTTCAGAAGACGTATGACA+TGG - chr6.4:16658411-16658430 MS.gene42036:CDS 40.0%
TCTTCATTGTCTGAACAGCA+TGG + chr6.4:16660222-16660241 None:intergenic 40.0%
! ATCCATGGATTCGATTTTGC+GGG + chr6.4:16659956-16659975 None:intergenic 40.0%
! GTAGGTCTTAATTTTCGGTG+TGG - chr6.4:16659068-16659087 MS.gene42036:intron 40.0%
! TGCTGTGTTTGAAATAGGCT+GGG + chr6.4:16658531-16658550 None:intergenic 40.0%
! TGTGAATTGGTGGGTGATTT+AGG - chr6.4:16659094-16659113 MS.gene42036:intron 40.0%
! TTCAGCTGGAAGATTTTCAG+CGG - chr6.4:16659842-16659861 MS.gene42036:intron 40.0%
! TTGCTGTGTTTGAAATAGGC+TGG + chr6.4:16658532-16658551 None:intergenic 40.0%
!! CATCTGGATCTTGGTAATGA+TGG - chr6.4:16659161-16659180 MS.gene42036:intron 40.0%
AACAGCATGGACTTTCGGTA+AGG + chr6.4:16660209-16660228 None:intergenic 45.0%
AAGGTCAACCAAGCTACCTT+CGG + chr6.4:16660037-16660056 None:intergenic 45.0%
AATCAGATCCTGATCGTTGG+GGG - chr6.4:16659611-16659630 MS.gene42036:intron 45.0%
ACTTCAGGAAGCAAATCTGG+CGG + chr6.4:16659442-16659461 None:intergenic 45.0%
AGAAACGATCTCATCTGTGG+TGG + chr6.4:16658446-16658465 None:intergenic 45.0%
AGAAGAACGCGAGAGAATTC+AGG + chr6.4:16658508-16658527 None:intergenic 45.0%
CCAAGAAACGATCTCATCTG+TGG + chr6.4:16658449-16658468 None:intergenic 45.0%
CCACAGATGAGATCGTTTCT+TGG - chr6.4:16658446-16658465 MS.gene42036:CDS 45.0%
CGTAGACATCTCAAGCATCT+AGG - chr6.4:16659316-16659335 MS.gene42036:intron 45.0%
GAAACGATCTCATCTGTGGT+GGG + chr6.4:16658445-16658464 None:intergenic 45.0%
GACTGTGGATAGGTTTCTTG+CGG + chr6.4:16659690-16659709 None:intergenic 45.0%
GGATGACTATGACTGTGGAT+AGG + chr6.4:16659700-16659719 None:intergenic 45.0%
GTCTGAACAGCATGGACTTT+CGG + chr6.4:16660214-16660233 None:intergenic 45.0%
TCTGGATCTACAGTAGAACC+CGG + chr6.4:16659724-16659743 None:intergenic 45.0%
TGAAGCCTCCTTTGGATCAA+CGG + chr6.4:16660124-16660143 None:intergenic 45.0%
TGAGACAGAAAAGCTTCAGC+TGG - chr6.4:16659828-16659847 MS.gene42036:intron 45.0%
TTCCCGCAAAATCGAATCCA+TGG - chr6.4:16659951-16659970 MS.gene42036:intron 45.0%
TTCGTTGTTTGTGTCGTGTC+TGG + chr6.4:16658950-16658969 None:intergenic 45.0%
TTTCTGCAGCATCTGGATCT+TGG - chr6.4:16659152-16659171 MS.gene42036:intron 45.0%
! CTTCTTTGAGAAGGCTCTTC+AGG - chr6.4:16659906-16659925 MS.gene42036:intron 45.0%
! CTTTCTGAAGGAGAACTGAC+AGG - chr6.4:16660078-16660097 MS.gene42036:CDS 45.0%
AACTGACCCCGTTGATCCAA+AGG - chr6.4:16660113-16660132 MS.gene42036:CDS 50.0%
AAGCCTCCTTTGGATCAACG+GGG + chr6.4:16660122-16660141 None:intergenic 50.0%
ACGTTACCTGAAGCCTCCTT+TGG + chr6.4:16660132-16660151 None:intergenic 50.0%
AGAACGCGAGAGAATTCAGG+AGG + chr6.4:16658505-16658524 None:intergenic 50.0%
AGATCCAGAAAGGGCAGCAT+TGG - chr6.4:16659735-16659754 MS.gene42036:intron 50.0%
CACAGTCATAGTCATCCTCC+GGG - chr6.4:16659703-16659722 MS.gene42036:intron 50.0%
CATACCTTCTCTGCTTCCGT+CGG + chr6.4:16658383-16658402 None:intergenic 50.0%
CCACAGTCATAGTCATCCTC+CGG - chr6.4:16659702-16659721 MS.gene42036:intron 50.0%
CTACGATGCAGAAGAGAAGC+AGG + chr6.4:16659301-16659320 None:intergenic 50.0%
GAACGCGAGAGAATTCAGGA+GGG + chr6.4:16658504-16658523 None:intergenic 50.0%
GAAGCCTCCTTTGGATCAAC+GGG + chr6.4:16660123-16660142 None:intergenic 50.0%
GCAAGGACACTCCTCAGATT+AGG + chr6.4:16659045-16659064 None:intergenic 50.0%
GTGTGGGTGTGTGAATTGGT+GGG - chr6.4:16659085-16659104 MS.gene42036:intron 50.0%
GTTGATCCAAAGGAGGCTTC+AGG - chr6.4:16660123-16660142 MS.gene42036:CDS 50.0%
TCCTCAGATTAGGTGTGTCC+TGG + chr6.4:16659035-16659054 None:intergenic 50.0%
TTCTCCAATGCTGCCCTTTC+TGG + chr6.4:16659742-16659761 None:intergenic 50.0%
! ATTGAGCTGACGGATGAAGC+TGG + chr6.4:16658560-16658579 None:intergenic 50.0%
! GATTGGAAGGCATGGAGAAG+AGG - chr6.4:16659866-16659885 MS.gene42036:intron 50.0%
! GATTTTCAGCGGCGATTGGA+AGG - chr6.4:16659853-16659872 MS.gene42036:intron 50.0%
! GCAGTGGCTGATGATGATTG+TGG + chr6.4:16658358-16658377 None:intergenic 50.0%
! GGAAGATTTTCAGCGGCGAT+TGG - chr6.4:16659849-16659868 MS.gene42036:intron 50.0%
! TTCGGTGTGGGTGTGTGAAT+TGG - chr6.4:16659081-16659100 MS.gene42036:intron 50.0%
ACCAAGCTACCTTCGGCAAC+AGG + chr6.4:16660030-16660049 None:intergenic 55.0%
CAGGAAGCAAATCTGGCGGT+GGG + chr6.4:16659438-16659457 None:intergenic 55.0%
CCGGAGGATGACTATGACTG+TGG + chr6.4:16659705-16659724 None:intergenic 55.0%
CGACGGAAGCAGAGAAGGTA+TGG - chr6.4:16658381-16658400 MS.gene42036:CDS 55.0%
GATTAGGTGTGTCCTGGCGT+CGG + chr6.4:16659029-16659048 None:intergenic 55.0%
GCCAGGACACACCTAATCTG+AGG - chr6.4:16659031-16659050 MS.gene42036:intron 55.0%
GGATCTACAGTAGAACCCGG+AGG + chr6.4:16659721-16659740 None:intergenic 55.0%
GGTGTGGGTGTGTGAATTGG+TGG - chr6.4:16659084-16659103 MS.gene42036:intron 55.0%
TCAGGAAGCAAATCTGGCGG+TGG + chr6.4:16659439-16659458 None:intergenic 55.0%
TCCAAGTCGAAGCAGCTGCT+CGG - chr6.4:16660151-16660170 MS.gene42036:CDS 55.0%
TGACCCCGTTGATCCAAAGG+AGG - chr6.4:16660116-16660135 MS.gene42036:CDS 55.0%
TTGGCAAACCCCCAACGATC+AGG + chr6.4:16659622-16659641 None:intergenic 55.0%
!! GAGGAGTGTCCTTGCTTCGT+AGG - chr6.4:16659050-16659069 MS.gene42036:intron 55.0%
CACATGCAGCCTGTTGCCGA+AGG - chr6.4:16660018-16660037 MS.gene42036:CDS 60.0%
GCCTGTTGCCGAAGGTAGCT+TGG - chr6.4:16660026-16660045 MS.gene42036:CDS 60.0%
TCAGCGGCGATTGGAAGGCA+TGG - chr6.4:16659858-16659877 MS.gene42036:intron 60.0%
TCATCAGCCACTGCTGCCGA+CGG - chr6.4:16658364-16658383 MS.gene42036:CDS 60.0%
GCCGAGCAGCTGCTTCGACT+TGG + chr6.4:16660155-16660174 None:intergenic 65.0%
GCTGCCGACGGAAGCAGAGA+AGG - chr6.4:16658376-16658395 MS.gene42036:CDS 65.0%
GTCGAAGCAGCTGCTCGGCA+AGG - chr6.4:16660156-16660175 MS.gene42036:CDS 65.0%
TCTGCTTCCGTCGGCAGCAG+TGG + chr6.4:16658374-16658393 None:intergenic 65.0%
! CGGTGTTAGACACCGACGCC+AGG - chr6.4:16659014-16659033 MS.gene42036:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 16658343 16660257 16658343 ID=MS.gene42036
chr6.4 mRNA 16658343 16660257 16658343 ID=MS.gene42036.t1;Parent=MS.gene42036
chr6.4 exon 16660015 16660257 16660015 ID=MS.gene42036.t1.exon1;Parent=MS.gene42036.t1
chr6.4 CDS 16660015 16660257 16660015 ID=cds.MS.gene42036.t1;Parent=MS.gene42036.t1
chr6.4 exon 16658343 16658999 16658343 ID=MS.gene42036.t1.exon2;Parent=MS.gene42036.t1
chr6.4 CDS 16658343 16658999 16658343 ID=cds.MS.gene42036.t1;Parent=MS.gene42036.t1
Gene Sequence

>MS.gene42036

ATGAATTCTGCACCACAATCATCATCAGCCACTGCTGCCGACGGAAGCAGAGAAGGTATGGCACCGTTTCTTCAGAAGACGTATGACATGGTAGATGATCCCACCACAGATGAGATCGTTTCTTGGAGCTCTGATAACAAAAGCTTCATCGTTTGGAACCCTCCTGAATTCTCTCGCGTTCTTCTCCCAGCCTATTTCAAACACAGCAATTTCTCCAGCTTCATCCGTCAGCTCAATACCTATGTAAGTCAATTCACGCTATAAGCTTCTTTTGATTATTTCATTGTCACTTTGTATGATCAATTAGTTATTGTCTTTCACTGTGTTTTTGCTTGATTGTTTGTTGTATTTGTATGATTTGGTTTCATTGTGTCTTCATCGAATTTCAATTAGTGGATACTGATTATTATGATGATCATGATCATTAAGAGATTGATGAAAAAAATGATTAGCAAGATAAGAATTAGGTACAACTTTAGATTTGGATTATTTCATTGAGTTTAGTTGGAGATACAAAATGTTGAGACATGAGAATGTTGAAAGATGGATGAGATATTATAGTCTTGTATGGCACTTTTATTCTATGAAACATAAAACATAGACACCAGACACGACACAAACAACGAAACGCAAACAACGACAATGACATAATTCAGTATAATCATATGTGTCGGTGTTAGACACCGACGCCAGGACACACCTAATCTGAGGAGTGTCCTTGCTTCGTAGGTCTTAATTTTCGGTGTGGGTGTGTGAATTGGTGGGTGATTTAGGACTCTAAATGCACTCAAGAAAAACGATCAAAAAAATTTCTGCAGCATCTGGATCTTGGTAATGATGGTACATCGAATTATGAATAAAACCATCAATTTAGTCTCTGAATATCACACTATTTCCGGTTTAGTTCCTGAACTATATAAATATGATAAGTAGTTCCTGATTTTTATTGTTTAATCCTGCTTCTCTTCTGCATCGTAGACATCTCAAGCATCTAGGTTTTCATTTATTGAACTTGTTGAAATTGATATCTAAGTCTTAGATAGGGTTGAAAAATAGAAATCATGCACTTATGGCAATGATTAAAAACTAAGTCCCACCGCCAGATTTGCTTCCTGAAGTTTTTGTTTAATAAAGAATATTCTTGTAGAGATTTAATGGTAAATCGTTCAAAATAGGTCTTATGGATGTAATGATAAATAATTGTCTAAGAATGTATGTTTTAATTTGTTACGGTTTGTTTACGTGTGCTTTTTATCAGGGATTTCGAAAATCAGATCCTGATCGTTGGGGGTTTGCCAATGAAAAATTTATAAAAGATCAAAAACATCTTCTGACGGATATACACCGCAAGAAACCTATCCACAGTCATAGTCATCCTCCGGGTTCTACTGTAGATCCAGAAAGGGCAGCATTGGAGAAAGAAATAGAGAAGCTGTCTCGCAAGAAAAATGCCCTTCAATCCAAACTCTTAAGCTATAATTATCTTGAGACAGAAAAGCTTCAGCTGGAAGATTTTCAGCGGCGATTGGAAGGCATGGAGAAGAGGCAGACAAATTTGCAGAACTTCTTTGAGAAGGCTCTTCAGGATTCTTTCATTGTTGAACTTCTTTCCCGCAAAATCGAATCCATGGATTTGGAAACTTATAATAAGAAAAGGCGATTGTCTCAGGTTGATCACATGCAGCCTGTTGCCGAAGGTAGCTTGGTTGACCTTCCTAGCAATTTCTCTCAGAATCTTTCTGAAGGAGAACTGACAGGAATACAGACAAGAACTGACCCCGTTGATCCAAAGGAGGCTTCAGGTAACGTCCAAGTCGAAGCAGCTGCTCGGCAAGGAGTGAATGATGTGTTTTGGGAACAATTCCTTACCGAAAGTCCATGCTGTTCAGACAATGAAGAGGAAATGTTCAACTAA
Protein sequence

>MS.gene42036.t1

MNSAPQSSSATAADGSREGMAPFLQKTYDMVDDPTTDEIVSWSSDNKSFIVWNPPEFSRVLLPAYFKHSNFSSFIRQLNTYGFRKSDPDRWGFANEKFIKDQKHLLTDIHRKKPIHSHSHPPGSTVDPERAALEKEIEKLSRKKNALQSKLLSYNYLETEKLQLEDFQRRLEGMEKRQTNLQNFFEKALQDSFIVELLSRKIESMDLETYNKKRRLSQVDHMQPVAEGSLVDLPSNFSQNLSEGELTGIQTRTDPVDPKEASGNVQVEAAARQGVNDVFWEQFLTESPCCSDNEEEMFN