AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42308

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42308.t1 MTR_3g112300 91.443 596 38 5 1 592 1 587 0.0 1116
MS.gene42308.t1 MTR_3g112300 89.107 459 44 2 1 459 1 453 0.0 852
MS.gene42308.t1 MTR_3g112300 93.103 87 4 2 508 592 431 517 5.49e-43 161
MS.gene42308.t1 MTR_3g112300 93.112 392 27 0 1 392 1 392 0.0 768
MS.gene42308.t1 MTR_3g112300 92.722 371 27 0 1 371 1 371 0.0 725
MS.gene42308.t1 MTR_1g023050 61.420 648 173 19 1 592 1 627 0.0 697
MS.gene42308.t1 MTR_1g057790 55.556 279 109 5 162 430 161 434 2.84e-85 279
MS.gene42308.t1 MTR_4g051532 55.357 280 112 5 162 434 217 490 8.46e-85 279
MS.gene42308.t1 MTR_7g106320 54.717 265 110 4 163 420 172 433 2.32e-80 266
MS.gene42308.t1 MTR_8g078480 50.000 274 120 5 158 420 384 651 4.21e-80 269
MS.gene42308.t1 MTR_5g018860 50.183 273 119 4 161 423 337 602 4.38e-80 268
MS.gene42308.t1 MTR_8g098815 47.203 286 117 7 141 408 163 432 7.37e-75 250
MS.gene42308.t1 MTR_3g112290 45.392 293 126 6 156 431 159 434 2.73e-70 233
MS.gene42308.t1 MTR_7g065050 36.795 337 176 8 158 489 133 437 7.51e-60 207
MS.gene42308.t1 MTR_4g019450 42.366 262 124 3 158 415 192 430 2.27e-58 206
MS.gene42308.t1 MTR_2g095050 39.550 311 141 6 158 443 244 532 4.93e-57 202
MS.gene42308.t1 MTR_1g016490 39.847 261 145 6 159 414 240 493 4.74e-44 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42308.t1 AT1G75410 48.148 405 178 8 35 430 50 431 1.00e-106 331
MS.gene42308.t1 AT1G75410 48.148 405 178 8 35 430 50 431 1.00e-106 331
MS.gene42308.t1 AT1G19700 48.434 415 169 13 34 428 46 435 1.51e-106 331
MS.gene42308.t1 AT1G19700 48.434 415 169 13 34 428 46 435 1.51e-106 331
MS.gene42308.t1 AT1G19700 48.434 415 169 13 34 428 46 435 1.51e-106 331
MS.gene42308.t1 AT1G19700 48.434 415 169 13 34 428 46 435 1.51e-106 331
MS.gene42308.t1 AT1G19700 48.434 415 169 13 34 428 46 435 1.51e-106 331
MS.gene42308.t1 AT2G16400 44.301 465 171 14 141 592 93 482 2.64e-106 329
MS.gene42308.t1 AT4G34610 44.898 441 179 15 165 592 143 532 2.78e-101 317
MS.gene42308.t1 AT4G34610 44.898 441 179 15 165 592 143 532 2.78e-101 317
MS.gene42308.t1 AT4G34610 44.898 441 179 15 165 592 143 532 2.78e-101 317
MS.gene42308.t1 AT4G34610 44.898 441 179 15 165 592 143 532 2.78e-101 317
MS.gene42308.t1 AT1G75410 46.049 367 166 8 35 392 50 393 1.44e-85 272
MS.gene42308.t1 AT2G23760 52.290 262 103 5 165 413 240 492 3.89e-78 259
MS.gene42308.t1 AT2G23760 52.290 262 103 5 165 413 240 492 3.89e-78 259
MS.gene42308.t1 AT2G23760 52.290 262 103 5 165 413 240 492 3.89e-78 259
MS.gene42308.t1 AT2G35940 39.765 425 205 12 158 536 186 605 3.64e-77 258
MS.gene42308.t1 AT2G35940 39.765 425 205 12 158 536 186 605 3.64e-77 258
MS.gene42308.t1 AT2G35940 39.765 425 205 12 158 536 186 605 3.64e-77 258
MS.gene42308.t1 AT4G36870 49.064 267 113 5 161 414 311 567 3.88e-74 251
MS.gene42308.t1 AT4G36870 49.064 267 113 5 161 414 311 567 3.88e-74 251
MS.gene42308.t1 AT2G23760 43.631 314 103 6 165 413 240 544 4.41e-68 234
MS.gene42308.t1 AT2G27220 46.185 249 101 5 168 415 82 298 6.70e-66 221
MS.gene42308.t1 AT2G27220 46.185 249 101 5 168 415 82 298 6.70e-66 221
MS.gene42308.t1 AT2G27220 43.446 267 100 7 168 415 82 316 2.39e-62 213
MS.gene42308.t1 AT5G41410 39.319 323 146 7 232 538 265 553 2.23e-61 214
MS.gene42308.t1 AT1G75430 41.241 274 130 7 163 419 17 276 7.70e-61 204
MS.gene42308.t1 AT4G36870 45.935 246 110 5 161 393 311 546 1.26e-58 205
MS.gene42308.t1 AT4G36870 45.902 244 109 5 161 391 311 544 9.90e-58 203
MS.gene42308.t1 AT5G02030 42.701 274 120 6 158 415 166 418 1.06e-54 195
MS.gene42308.t1 AT2G27990 43.346 263 118 8 158 415 258 494 4.60e-51 185
MS.gene42308.t1 AT4G32980 37.736 265 141 5 156 414 195 441 3.31e-44 164
MS.gene42308.t1 AT4G32980 37.736 265 141 5 156 414 195 441 3.31e-44 164
MS.gene42308.t1 AT1G75430 34.071 226 118 7 163 371 17 228 6.68e-29 117
MS.gene42308.t1 AT2G27990 36.073 219 109 8 158 371 258 450 2.46e-25 109

Find 121 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTTTGGACATGGTTGC+TGG 0.171008 3.2:-89569727 MS.gene42308:CDS
GACTATCATTTCACAGATTC+AGG 0.242730 3.2:-89568538 MS.gene42308:CDS
TTAATGCACGTGTGCGTCTT+TGG 0.261906 3.2:-89569050 MS.gene42308:CDS
AGAGATTAATAGTAATAAAT+TGG 0.292200 3.2:-89568923 MS.gene42308:CDS
GCAAATTGTGGTGTCATCTT+TGG 0.309506 3.2:-89569740 MS.gene42308:CDS
CCCAACCAAGCATTCTCTTC+AGG 0.317247 3.2:-89570801 MS.gene42308:CDS
CATCTTTGGACATGGTTGCT+GGG 0.322770 3.2:-89569726 MS.gene42308:CDS
CCTTAAGATATTGAGAGTTT+AGG 0.323728 3.2:+89570319 None:intergenic
CCTAAACTCTCAATATCTTA+AGG 0.324664 3.2:-89570319 MS.gene42308:CDS
ATCCAACAAATTCTGTCTTT+CGG 0.327791 3.2:+89570104 None:intergenic
TTTCCATTCCATGTTGCTTT+CGG 0.331914 3.2:+89570262 None:intergenic
CTCCAAGGTAAAAGAGATTA+TGG 0.332690 3.2:-89568949 MS.gene42308:CDS
GAAATATACAAAGAAGAGTT+TGG 0.368762 3.2:-89569012 MS.gene42308:CDS
CGCAATGCAAGTGTAGTATA+TGG 0.380736 3.2:+89569688 None:intergenic
CACATATGATTTAACAGAGT+TGG 0.383789 3.2:-89568728 MS.gene42308:CDS
AAGTGATTCAAAGGAACCTT+GGG 0.404482 3.2:-89569612 MS.gene42308:CDS
ATTCTCTTCAGGGTCCTATA+TGG 0.406017 3.2:-89570790 MS.gene42308:CDS
CAGTCTATGCAAATAACTTC+GGG 0.408568 3.2:-89570174 MS.gene42308:CDS
GTTGCGAAAACACTCCCTTT+TGG 0.409394 3.2:+89570395 None:intergenic
TATGGTATCAGGTGGCAAAT+TGG 0.411576 3.2:-89569077 MS.gene42308:intron
AATGTGATAGATTGTGGAAA+TGG 0.412066 3.2:-89568820 MS.gene42308:CDS
TTGAGAGTTTAGGAAAATGT+TGG 0.412411 3.2:+89570329 None:intergenic
CGAAACCGGTGTCATTATAC+TGG 0.413951 3.2:+89570532 None:intergenic
GGAAAGCAACAACACATGTT+TGG 0.421673 3.2:-89568517 MS.gene42308:CDS
TCATTATACTGGTATTGAAA+TGG 0.422426 3.2:+89570543 None:intergenic
CCAACCAAGCATTCTCTTCA+GGG 0.428447 3.2:-89570800 MS.gene42308:CDS
AACTGGTTTGACAAGGAATC+AGG 0.432153 3.2:-89569215 MS.gene42308:intron
ACATATGATTTAACAGAGTT+GGG 0.435731 3.2:-89568727 MS.gene42308:CDS
CTGTTTCAATACTTCGTGCT+TGG 0.440367 3.2:-89569468 MS.gene42308:CDS
TCCTTTGAATCACTTGAACT+TGG 0.447044 3.2:+89569620 None:intergenic
ATTCATCAGCGATACGAAAC+CGG 0.447145 3.2:+89570518 None:intergenic
ATAATGCTAGCTAGGCAAAC+TGG 0.452403 3.2:-89569232 MS.gene42308:CDS
CCCTGAAGAGAATGCTTGGT+TGG 0.454991 3.2:+89570800 None:intergenic
TTGTATATTTCCTCAACCAT+TGG 0.456824 3.2:+89569024 None:intergenic
AGACATGCTTTCCATGCATT+CGG 0.460937 3.2:+89570440 None:intergenic
TTAGATATAGTAGTTCCCTT+TGG 0.461116 3.2:+89570483 None:intergenic
CAAAAGGCACTTCAGCATCT+TGG 0.463824 3.2:-89569538 MS.gene42308:CDS
GGAGGGACAGATGCATTAGA+AGG 0.464657 3.2:+89570564 None:intergenic
CAAATTCTGTCTTTCGGCTG+AGG 0.466044 3.2:+89570110 None:intergenic
TGCATGGAAAGCATGTCTTC+CGG 0.466072 3.2:-89570435 MS.gene42308:CDS
ATCTTTGGACATGGTTGCTG+GGG 0.467421 3.2:-89569725 MS.gene42308:CDS
CAGAGAAAATAATGCTAGCT+AGG 0.467771 3.2:-89569240 MS.gene42308:CDS
ACCAAGTTTAACACACCTAT+AGG 0.472281 3.2:-89570699 MS.gene42308:CDS
AGCCGAAAGACAGAATTTGT+TGG 0.478829 3.2:-89570106 MS.gene42308:CDS
CGAGGTGAACATATCCATAT+AGG 0.479681 3.2:+89570776 None:intergenic
ATAGTCACTGTGTCGCCTAT+AGG 0.482382 3.2:+89570684 None:intergenic
CAAGTGATTCAAAGGAACCT+TGG 0.483068 3.2:-89569613 MS.gene42308:CDS
TACATGTTGTCTAGTGGAAG+AGG 0.483872 3.2:+89568849 None:intergenic
CTCCTCAGATCACTGCCTGC+AGG 0.488491 3.2:-89570367 MS.gene42308:CDS
GCCTATAGGTGTGTTAAACT+TGG 0.490982 3.2:+89570698 None:intergenic
GGTAATGTCATGAAATCCTC+CGG 0.496563 3.2:+89570416 None:intergenic
GGATATCTCACAAGAAGGAT+TGG 0.496624 3.2:+89570131 None:intergenic
TCAGTCTATGCAAATAACTT+CGG 0.500629 3.2:-89570175 MS.gene42308:CDS
TCACAATGTCTCTCTTGCAT+TGG 0.504072 3.2:-89568674 MS.gene42308:CDS
GGATTGGAATTAGAATCATT+AGG 0.508994 3.2:+89570147 None:intergenic
AGCATTGGAATGCTCATCAA+TGG 0.509427 3.2:+89570656 None:intergenic
CCAACAGCTTAGGCAACAAA+AGG 0.510811 3.2:-89569554 MS.gene42308:CDS
CGAGACTAAGAGATAACTCT+TGG 0.512889 3.2:+89570598 None:intergenic
ACACCACAATTTGCATTCGA+TGG 0.516116 3.2:+89569749 None:intergenic
TACATGAGAGCTGTAAGCAT+TGG 0.517302 3.2:+89570641 None:intergenic
CCTGAAGAGAATGCTTGGTT+GGG 0.517326 3.2:+89570801 None:intergenic
GATTTCATGACATTACCAAA+AGG 0.521517 3.2:-89570410 MS.gene42308:CDS
TCATCTGAAAATACACTCCA+AGG 0.523657 3.2:-89568964 MS.gene42308:CDS
TGTCCGAAAGCAACATGGAA+TGG 0.523675 3.2:-89570265 MS.gene42308:CDS
CAAGAGTTATCTCTTAGTCT+CGG 0.528666 3.2:-89570597 MS.gene42308:CDS
AATTCTTGTCGTCTTCCAAA+GGG 0.529186 3.2:-89570498 MS.gene42308:CDS
GAATTCTTGTCGTCTTCCAA+AGG 0.533875 3.2:-89570499 MS.gene42308:CDS
TAAGCTGTTGGTCCACGTAC+CGG 0.539458 3.2:+89569566 None:intergenic
AAGAGTTATCTCTTAGTCTC+GGG 0.540585 3.2:-89570596 MS.gene42308:CDS
AGGACCCTGAAGAGAATGCT+TGG 0.541911 3.2:+89570796 None:intergenic
GTATTCCTTCTTGTTCCCCA+AGG 0.544341 3.2:+89569596 None:intergenic
ATTTCATGACATTACCAAAA+GGG 0.544569 3.2:-89570409 MS.gene42308:CDS
TCTTTGGACATGGTTGCTGG+GGG 0.546073 3.2:-89569724 MS.gene42308:CDS
ACCAAGTTCAAGTGATTCAA+AGG 0.549397 3.2:-89569621 MS.gene42308:CDS
GGTACGTGGACCAACAGCTT+AGG 0.555219 3.2:-89569564 MS.gene42308:CDS
TTGGTGTTATGCGGCAAGCA+TGG 0.558919 3.2:-89569519 MS.gene42308:CDS
CACTGCCTGCAGGATTCACA+AGG 0.565086 3.2:-89570357 MS.gene42308:CDS
ATTGAAAAGTGCCGAATGCA+TGG 0.565403 3.2:-89570451 MS.gene42308:CDS
CAACTCTGTTAAATCATATG+TGG 0.566114 3.2:+89568729 None:intergenic
TAATAATAATAATCAGACAT+TGG 0.566437 3.2:-89568590 MS.gene42308:CDS
TTCAGCATCTTGGTGTTATG+CGG 0.567244 3.2:-89569528 MS.gene42308:CDS
GATAATCCCAAAGATAGTGA+TGG 0.570048 3.2:-89570210 MS.gene42308:CDS
TTATACTGGTATTGAAATGG+AGG 0.572976 3.2:+89570546 None:intergenic
AAGAAGGAATACCTCGTCTC+CGG 0.574564 3.2:-89569585 MS.gene42308:CDS
AGGAACCTTGGGGAACAAGA+AGG 0.579072 3.2:-89569601 MS.gene42308:CDS
AATACCTCGTCTCCGGTACG+TGG 0.579283 3.2:-89569578 MS.gene42308:CDS
TCTTTGGAAACCAATGGTTG+AGG 0.583656 3.2:-89569034 MS.gene42308:CDS
TTGAGAAACTGTGAAAGCGA+TGG 0.592333 3.2:-89568649 MS.gene42308:CDS
GGTAACTTTACAACTGTGGA+TGG 0.593227 3.2:-89568706 MS.gene42308:CDS
GTCTATGCAAATAACTTCGG+GGG 0.594987 3.2:-89570172 MS.gene42308:CDS
ATGAATGGCATCATCAGATG+TGG 0.596558 3.2:+89568621 None:intergenic
CAATACCAGTATAATGACAC+CGG 0.603044 3.2:-89570537 MS.gene42308:CDS
GAAAATTGCATGGCAACCAA+AGG 0.604743 3.2:-89568798 MS.gene42308:CDS
GTTAATGTCCGAAAGCAACA+TGG 0.605991 3.2:-89570270 MS.gene42308:CDS
TCAATATCTTAAGGCTACAC+AGG 0.606571 3.2:-89570310 MS.gene42308:CDS
GATTATCCAATCCAATGTTG+CGG 0.607575 3.2:+89570226 None:intergenic
GGCAGTGATCTGAGGAGTGA+GGG 0.610386 3.2:+89570373 None:intergenic
TTTACAACTGTGGATGGTGG+TGG 0.611372 3.2:-89568700 MS.gene42308:CDS
GGTTGATACCAGTTACCCCA+AGG 0.615136 3.2:-89569266 MS.gene42308:intron
AACTTTACAACTGTGGATGG+TGG 0.616544 3.2:-89568703 MS.gene42308:CDS
CTAGGCAAACTGGTTTGACA+AGG 0.616619 3.2:-89569222 MS.gene42308:CDS
GCTGAGGATATCTCACAAGA+AGG 0.617588 3.2:+89570126 None:intergenic
GATTCATTGTAGTAATTGTG+AGG 0.618741 3.2:+89570741 None:intergenic
AGGCAGTGATCTGAGGAGTG+AGG 0.618773 3.2:+89570372 None:intergenic
TGGTCCACGTACCGGAGACG+AGG 0.620141 3.2:+89569574 None:intergenic
ATCCTGCAGGCAGTGATCTG+AGG 0.622811 3.2:+89570365 None:intergenic
TAGTAATTGTGAGGTAACAA+AGG 0.627033 3.2:+89570750 None:intergenic
TGTGGTGTCATCTTTGGACA+TGG 0.628842 3.2:-89569734 MS.gene42308:CDS
GTTGGGTAACTTTACAACTG+TGG 0.629995 3.2:-89568710 MS.gene42308:CDS
TATGGTTACCTCATCCAACA+TGG 0.631595 3.2:+89570062 None:intergenic
TGTGGAAATGGAAAATTGCA+TGG 0.642335 3.2:-89568808 MS.gene42308:CDS
TGTGCGTCTTTGGAAACCAA+TGG 0.645810 3.2:-89569040 MS.gene42308:CDS
ATGCAAGAGAGACATTGTGA+TGG 0.664466 3.2:+89568677 None:intergenic
TATACTGGTATTGAAATGGA+GGG 0.666797 3.2:+89570547 None:intergenic
GTGAGGTAACAAAGGACTCG+AGG 0.675570 3.2:+89570758 None:intergenic
CTGCCATCGAATGCAAATTG+TGG 0.686632 3.2:-89569752 MS.gene42308:CDS
ATGGAAAGCATGTCTTCCGG+AGG 0.687343 3.2:-89570432 MS.gene42308:CDS
TCTATGCAAATAACTTCGGG+GGG 0.707179 3.2:-89570171 MS.gene42308:CDS
AGTCTATGCAAATAACTTCG+GGG 0.713697 3.2:-89570173 MS.gene42308:CDS
AGTGATTCAAAGGAACCTTG+GGG 0.732244 3.2:-89569611 MS.gene42308:CDS
CAAGCATGGAGACCTCAGAG+AGG 0.733220 3.2:-89569505 MS.gene42308:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGATTAATAGTAATAAAT+TGG - chr3.2:89570367-89570386 MS.gene42308:CDS 15.0%
!! ATACAAATTAAATCCATAAA+AGG + chr3.2:89569274-89569293 None:intergenic 15.0%
!! TAATAATAATAATCAGACAT+TGG - chr3.2:89570700-89570719 MS.gene42308:CDS 15.0%
!!! ATCTTTTAATTTATATGGTA+TGG - chr3.2:89570195-89570214 MS.gene42308:CDS 15.0%
!!! ATTATTATGTCTTTTATGAA+TGG + chr3.2:89570687-89570706 None:intergenic 15.0%
!!! TTATCATATCTAACTTTTAT+TGG - chr3.2:89569358-89569377 MS.gene42308:intron 15.0%
!! AAATTAAATCCATAAAAGGA+AGG + chr3.2:89569270-89569289 None:intergenic 20.0%
!! GAAAATATAAAATTGAGTGA+AGG + chr3.2:89569908-89569927 None:intergenic 20.0%
!! GTATTATAAAGTAACACATA+CGG + chr3.2:89569869-89569888 None:intergenic 20.0%
!! TTCAACTTTCATTTCATAAT+TGG - chr3.2:89570003-89570022 MS.gene42308:intron 20.0%
!!! ATATTTTCAATTAGTTGCAA+TGG - chr3.2:89569920-89569939 MS.gene42308:intron 20.0%
!!! GTGTCATCTTTTAATTTATA+TGG - chr3.2:89570190-89570209 MS.gene42308:CDS 20.0%
! AATTTATATGGTATGGTATC+AGG - chr3.2:89570202-89570221 MS.gene42308:CDS 25.0%
! ACATATGATTTAACAGAGTT+GGG - chr3.2:89570563-89570582 MS.gene42308:CDS 25.0%
! AGTGCAAAAACTTTATCATA+AGG - chr3.2:89569310-89569329 MS.gene42308:intron 25.0%
! ATTTCATGACATTACCAAAA+GGG - chr3.2:89568881-89568900 MS.gene42308:CDS 25.0%
! GAAAAAAATGTGATAGATTG+TGG - chr3.2:89570464-89570483 MS.gene42308:CDS 25.0%
! GAAATATACAAAGAAGAGTT+TGG - chr3.2:89570278-89570297 MS.gene42308:CDS 25.0%
! TGAAGGATTAGAAATTTAGA+GGG + chr3.2:89569891-89569910 None:intergenic 25.0%
!! ATTATTTTCTCTGAATCCTT+GGG + chr3.2:89570043-89570062 None:intergenic 25.0%
!! TCATTATACTGGTATTGAAA+TGG + chr3.2:89568750-89568769 None:intergenic 25.0%
!!! AAGTATTGAAACAGAGTTTT+CGG + chr3.2:89569816-89569835 None:intergenic 25.0%
AATGTGATAGATTGTGGAAA+TGG - chr3.2:89570470-89570489 MS.gene42308:CDS 30.0%
ATCCAACAAATTCTGTCTTT+CGG + chr3.2:89569189-89569208 None:intergenic 30.0%
CAACTCTGTTAAATCATATG+TGG + chr3.2:89570564-89570583 None:intergenic 30.0%
CACATATGATTTAACAGAGT+TGG - chr3.2:89570562-89570581 MS.gene42308:CDS 30.0%
CCTAAACTCTCAATATCTTA+AGG - chr3.2:89568971-89568990 MS.gene42308:CDS 30.0%
CCTTAAGATATTGAGAGTTT+AGG + chr3.2:89568974-89568993 None:intergenic 30.0%
GAATAATTGTCACACACTAT+CGG + chr3.2:89569408-89569427 None:intergenic 30.0%
GATTCATTGTAGTAATTGTG+AGG + chr3.2:89568552-89568571 None:intergenic 30.0%
GATTTCATGACATTACCAAA+AGG - chr3.2:89568880-89568899 MS.gene42308:CDS 30.0%
GGATGCAATGCTAAATAATA+TGG + chr3.2:89569249-89569268 None:intergenic 30.0%
GGATTGGAATTAGAATCATT+AGG + chr3.2:89569146-89569165 None:intergenic 30.0%
GTGAAGGATTAGAAATTTAG+AGG + chr3.2:89569892-89569911 None:intergenic 30.0%
TAGTAATTGTGAGGTAACAA+AGG + chr3.2:89568543-89568562 None:intergenic 30.0%
TCAGTCTATGCAAATAACTT+CGG - chr3.2:89569115-89569134 MS.gene42308:intron 30.0%
TTAAAAGATGACACCACAAT+TGG + chr3.2:89570184-89570203 None:intergenic 30.0%
TTAGATATAGTAGTTCCCTT+TGG + chr3.2:89568810-89568829 None:intergenic 30.0%
TTGAGAGTTTAGGAAAATGT+TGG + chr3.2:89568964-89568983 None:intergenic 30.0%
TTGTATATTTCCTCAACCAT+TGG + chr3.2:89570269-89570288 None:intergenic 30.0%
! AGATTTTCCATCACTATCTT+TGG + chr3.2:89569090-89569109 None:intergenic 30.0%
! CATTATTTTCTCTGAATCCT+TGG + chr3.2:89570044-89570063 None:intergenic 30.0%
! CTCTTCTTTTATTCCATAGT+TGG - chr3.2:89569427-89569446 MS.gene42308:intron 30.0%
! GATTTTCCATCACTATCTTT+GGG + chr3.2:89569089-89569108 None:intergenic 30.0%
! TATACTGGTATTGAAATGGA+GGG + chr3.2:89568746-89568765 None:intergenic 30.0%
! TTATACTGGTATTGAAATGG+AGG + chr3.2:89568747-89568766 None:intergenic 30.0%
! TTATTTTCTCTGAATCCTTG+GGG + chr3.2:89570042-89570061 None:intergenic 30.0%
! TTTTTCTACATGTTGTCTAG+TGG + chr3.2:89570450-89570469 None:intergenic 30.0%
!! AGTATTGAAACAGAGTTTTC+GGG + chr3.2:89569815-89569834 None:intergenic 30.0%
AAAAATCCGCAACATTGGAT+TGG - chr3.2:89569058-89569077 MS.gene42308:CDS 35.0%
AAGAGTTATCTCTTAGTCTC+GGG - chr3.2:89568694-89568713 MS.gene42308:CDS 35.0%
AAGTGATTCAAAGGAACCTT+GGG - chr3.2:89569678-89569697 MS.gene42308:CDS 35.0%
AATTCTTGTCGTCTTCCAAA+GGG - chr3.2:89568792-89568811 MS.gene42308:CDS 35.0%
ACCAAGTTCAAGTGATTCAA+AGG - chr3.2:89569669-89569688 MS.gene42308:CDS 35.0%
ACCAAGTTTAACACACCTAT+AGG - chr3.2:89568591-89568610 MS.gene42308:CDS 35.0%
AGTCTATGCAAATAACTTCG+GGG - chr3.2:89569117-89569136 MS.gene42308:intron 35.0%
CAAAGGCTCAGCATAAAAAA+TGG - chr3.2:89570509-89570528 MS.gene42308:CDS 35.0%
CAAGAAAAAATCCGCAACAT+TGG - chr3.2:89569053-89569072 MS.gene42308:CDS 35.0%
CAAGAGTTATCTCTTAGTCT+CGG - chr3.2:89568693-89568712 MS.gene42308:CDS 35.0%
CAATACCAGTATAATGACAC+CGG - chr3.2:89568753-89568772 MS.gene42308:CDS 35.0%
CAGAGAAAATAATGCTAGCT+AGG - chr3.2:89570050-89570069 MS.gene42308:intron 35.0%
CAGTCTATGCAAATAACTTC+GGG - chr3.2:89569116-89569135 MS.gene42308:intron 35.0%
CTCCAAGGTAAAAGAGATTA+TGG - chr3.2:89570341-89570360 MS.gene42308:CDS 35.0%
CTGTAATCCTTTCTTTGTAC+TGG - chr3.2:89569486-89569505 MS.gene42308:CDS 35.0%
GACTATCATTTCACAGATTC+AGG - chr3.2:89570752-89570771 MS.gene42308:CDS 35.0%
GATAATCCCAAAGATAGTGA+TGG - chr3.2:89569080-89569099 MS.gene42308:intron 35.0%
GATTATCCAATCCAATGTTG+CGG + chr3.2:89569067-89569086 None:intergenic 35.0%
GCTGTCATAATAACCAACTA+TGG + chr3.2:89569443-89569462 None:intergenic 35.0%
TCAATATCTTAAGGCTACAC+AGG - chr3.2:89568980-89568999 MS.gene42308:CDS 35.0%
TCATCTGAAAATACACTCCA+AGG - chr3.2:89570326-89570345 MS.gene42308:CDS 35.0%
TCCTTTGAATCACTTGAACT+TGG + chr3.2:89569673-89569692 None:intergenic 35.0%
TGTGGAAATGGAAAATTGCA+TGG - chr3.2:89570482-89570501 MS.gene42308:CDS 35.0%
TTATATGGTATGGTATCAGG+TGG - chr3.2:89570205-89570224 MS.gene42308:CDS 35.0%
TTATTATGACAGCGATGGTT+TGG - chr3.2:89569450-89569469 MS.gene42308:CDS 35.0%
! ACTTGTTGCATTGCATAATC+AGG - chr3.2:89570150-89570169 MS.gene42308:CDS 35.0%
! GACCATAATCTCTTTTACCT+TGG + chr3.2:89570346-89570365 None:intergenic 35.0%
!! TTTCCATTCCATGTTGCTTT+CGG + chr3.2:89569031-89569050 None:intergenic 35.0%
!!! CATTTTTTATGCTGAGCCTT+TGG + chr3.2:89570511-89570530 None:intergenic 35.0%
AAAAACCTTGTGAATCCTGC+AGG + chr3.2:89568941-89568960 None:intergenic 40.0%
AACTTTACAACTGTGGATGG+TGG - chr3.2:89570587-89570606 MS.gene42308:CDS 40.0%
ACACCACAATTTGCATTCGA+TGG + chr3.2:89569544-89569563 None:intergenic 40.0%
AGACATGCTTTCCATGCATT+CGG + chr3.2:89568853-89568872 None:intergenic 40.0%
AGCCGAAAGACAGAATTTGT+TGG - chr3.2:89569184-89569203 MS.gene42308:intron 40.0%
AGTGATTCAAAGGAACCTTG+GGG - chr3.2:89569679-89569698 MS.gene42308:CDS 40.0%
ATAATGCTAGCTAGGCAAAC+TGG - chr3.2:89570058-89570077 MS.gene42308:intron 40.0%
ATGAATGGCATCATCAGATG+TGG + chr3.2:89570672-89570691 None:intergenic 40.0%
ATGCAAGAGAGACATTGTGA+TGG + chr3.2:89570616-89570635 None:intergenic 40.0%
ATTCATCAGCGATACGAAAC+CGG + chr3.2:89568775-89568794 None:intergenic 40.0%
ATTCTCTTCAGGGTCCTATA+TGG - chr3.2:89568500-89568519 MS.gene42308:CDS 40.0%
CAAGTGATTCAAAGGAACCT+TGG - chr3.2:89569677-89569696 MS.gene42308:CDS 40.0%
CCTACAACCAGTACAAAGAA+AGG + chr3.2:89569496-89569515 None:intergenic 40.0%
CGAGACTAAGAGATAACTCT+TGG + chr3.2:89568695-89568714 None:intergenic 40.0%
CGAGGTGAACATATCCATAT+AGG + chr3.2:89568517-89568536 None:intergenic 40.0%
CGCAATGCAAGTGTAGTATA+TGG + chr3.2:89569605-89569624 None:intergenic 40.0%
CTGTTTCAATACTTCGTGCT+TGG - chr3.2:89569822-89569841 MS.gene42308:intron 40.0%
GAAAATTGCATGGCAACCAA+AGG - chr3.2:89570492-89570511 MS.gene42308:CDS 40.0%
GAATTCTTGTCGTCTTCCAA+AGG - chr3.2:89568791-89568810 MS.gene42308:CDS 40.0%
GCCTATAGGTGTGTTAAACT+TGG + chr3.2:89568595-89568614 None:intergenic 40.0%
GGAAAGCAACAACACATGTT+TGG - chr3.2:89570773-89570792 MS.gene42308:CDS 40.0%
GGATATCTCACAAGAAGGAT+TGG + chr3.2:89569162-89569181 None:intergenic 40.0%
GGTAACTTTACAACTGTGGA+TGG - chr3.2:89570584-89570603 MS.gene42308:CDS 40.0%
GGTAATGTCATGAAATCCTC+CGG + chr3.2:89568877-89568896 None:intergenic 40.0%
GTCTATGCAAATAACTTCGG+GGG - chr3.2:89569118-89569137 MS.gene42308:intron 40.0%
GTTAATGTCCGAAAGCAACA+TGG - chr3.2:89569020-89569039 MS.gene42308:CDS 40.0%
GTTGGGTAACTTTACAACTG+TGG - chr3.2:89570580-89570599 MS.gene42308:CDS 40.0%
GTTGGTTATTATGACAGCGA+TGG - chr3.2:89569445-89569464 MS.gene42308:CDS 40.0%
TACATGAGAGCTGTAAGCAT+TGG + chr3.2:89568652-89568671 None:intergenic 40.0%
TACATGTTGTCTAGTGGAAG+AGG + chr3.2:89570444-89570463 None:intergenic 40.0%
TATGGTATCAGGTGGCAAAT+TGG - chr3.2:89570213-89570232 MS.gene42308:CDS 40.0%
TATGGTTACCTCATCCAACA+TGG + chr3.2:89569231-89569250 None:intergenic 40.0%
TCACAATGTCTCTCTTGCAT+TGG - chr3.2:89570616-89570635 MS.gene42308:CDS 40.0%
TCAGGTACAGTTACCAATTG+TGG - chr3.2:89570168-89570187 MS.gene42308:CDS 40.0%
TCTATGCAAATAACTTCGGG+GGG - chr3.2:89569119-89569138 MS.gene42308:intron 40.0%
TTGAGAAACTGTGAAAGCGA+TGG - chr3.2:89570641-89570660 MS.gene42308:CDS 40.0%
! AGCATTGGAATGCTCATCAA+TGG + chr3.2:89568637-89568656 None:intergenic 40.0%
! GACAAAGCTTTTGTCCATGT+TGG - chr3.2:89569214-89569233 MS.gene42308:intron 40.0%
! GCATTGCATCCTTCCTTTTA+TGG - chr3.2:89569258-89569277 MS.gene42308:intron 40.0%
! TCTTTGGAAACCAATGGTTG+AGG - chr3.2:89570256-89570275 MS.gene42308:CDS 40.0%
!! AACTGGTTTGACAAGGAATC+AGG - chr3.2:89570075-89570094 MS.gene42308:CDS 40.0%
!! ATTGAAAAGTGCCGAATGCA+TGG - chr3.2:89568839-89568858 MS.gene42308:CDS 40.0%
!! CCTTTCTTTGTACTGGTTGT+AGG - chr3.2:89569493-89569512 MS.gene42308:CDS 40.0%
!! GCAAATTGTGGTGTCATCTT+TGG - chr3.2:89569550-89569569 MS.gene42308:CDS 40.0%
!! TCTTTGTACTGGTTGTAGGT+AGG - chr3.2:89569497-89569516 MS.gene42308:CDS 40.0%
!! TTCAGCATCTTGGTGTTATG+CGG - chr3.2:89569762-89569781 MS.gene42308:CDS 40.0%
AAGAAGGAATACCTCGTCTC+CGG - chr3.2:89569705-89569724 MS.gene42308:CDS 45.0%
ATAGTCACTGTGTCGCCTAT+AGG + chr3.2:89568609-89568628 None:intergenic 45.0%
ATCTTTGGACATGGTTGCTG+GGG - chr3.2:89569565-89569584 MS.gene42308:CDS 45.0%
CAAATTCTGTCTTTCGGCTG+AGG + chr3.2:89569183-89569202 None:intergenic 45.0%
CATCTTTGGACATGGTTGCT+GGG - chr3.2:89569564-89569583 MS.gene42308:CDS 45.0%
CCAACAGCTTAGGCAACAAA+AGG - chr3.2:89569736-89569755 MS.gene42308:CDS 45.0%
CCAACCAAGCATTCTCTTCA+GGG - chr3.2:89568490-89568509 MS.gene42308:CDS 45.0%
CCTGAAGAGAATGCTTGGTT+GGG + chr3.2:89568492-89568511 None:intergenic 45.0%
CTGCCATCGAATGCAAATTG+TGG - chr3.2:89569538-89569557 MS.gene42308:CDS 45.0%
GCTGAGGATATCTCACAAGA+AGG + chr3.2:89569167-89569186 None:intergenic 45.0%
GTATTCCTTCTTGTTCCCCA+AGG + chr3.2:89569697-89569716 None:intergenic 45.0%
TCATCTTTGGACATGGTTGC+TGG - chr3.2:89569563-89569582 MS.gene42308:CDS 45.0%
TGTCCGAAAGCAACATGGAA+TGG - chr3.2:89569025-89569044 MS.gene42308:CDS 45.0%
TTAATGCACGTGTGCGTCTT+TGG - chr3.2:89570240-89570259 MS.gene42308:CDS 45.0%
! CCTTTTGTTGCCTAAGCTGT+TGG + chr3.2:89569739-89569758 None:intergenic 45.0%
! GCTTTTGTCCATGTTGGATG+AGG - chr3.2:89569220-89569239 MS.gene42308:CDS 45.0%
! GTTGCGAAAACACTCCCTTT+TGG + chr3.2:89568898-89568917 None:intergenic 45.0%
! TGCATGGAAAGCATGTCTTC+CGG - chr3.2:89568855-89568874 MS.gene42308:CDS 45.0%
! TGTGCGTCTTTGGAAACCAA+TGG - chr3.2:89570250-89570269 MS.gene42308:CDS 45.0%
! TTTACAACTGTGGATGGTGG+TGG - chr3.2:89570590-89570609 MS.gene42308:CDS 45.0%
!! CAAAAGGCACTTCAGCATCT+TGG - chr3.2:89569752-89569771 MS.gene42308:CDS 45.0%
!! CGAAACCGGTGTCATTATAC+TGG + chr3.2:89568761-89568780 None:intergenic 45.0%
!! CTAGGCAAACTGGTTTGACA+AGG - chr3.2:89570068-89570087 MS.gene42308:intron 45.0%
!! TGTGGTGTCATCTTTGGACA+TGG - chr3.2:89569556-89569575 MS.gene42308:CDS 45.0%
AGGAACCTTGGGGAACAAGA+AGG - chr3.2:89569689-89569708 MS.gene42308:CDS 50.0%
AGGACCCTGAAGAGAATGCT+TGG + chr3.2:89568497-89568516 None:intergenic 50.0%
CCCAACCAAGCATTCTCTTC+AGG - chr3.2:89568489-89568508 MS.gene42308:CDS 50.0%
CCCTGAAGAGAATGCTTGGT+TGG + chr3.2:89568493-89568512 None:intergenic 50.0%
CTCTGAATCCTTGGGGTAAC+TGG + chr3.2:89570035-89570054 None:intergenic 50.0%
GGAGGGACAGATGCATTAGA+AGG + chr3.2:89568729-89568748 None:intergenic 50.0%
GTGAGGTAACAAAGGACTCG+AGG + chr3.2:89568535-89568554 None:intergenic 50.0%
TAAGCTGTTGGTCCACGTAC+CGG + chr3.2:89569727-89569746 None:intergenic 50.0%
TCTTTGGACATGGTTGCTGG+GGG - chr3.2:89569566-89569585 MS.gene42308:CDS 50.0%
! ATGGAAAGCATGTCTTCCGG+AGG - chr3.2:89568858-89568877 MS.gene42308:CDS 50.0%
! GGTTGATACCAGTTACCCCA+AGG - chr3.2:89570024-89570043 MS.gene42308:intron 50.0%
!! TTGGTGTTATGCGGCAAGCA+TGG - chr3.2:89569771-89569790 MS.gene42308:CDS 50.0%
!! TTTTCGGGAAGTCCTCTCTG+AGG + chr3.2:89569800-89569819 None:intergenic 50.0%
AATACCTCGTCTCCGGTACG+TGG - chr3.2:89569712-89569731 MS.gene42308:CDS 55.0%
AGGCAGTGATCTGAGGAGTG+AGG + chr3.2:89568921-89568940 None:intergenic 55.0%
ATCCTGCAGGCAGTGATCTG+AGG + chr3.2:89568928-89568947 None:intergenic 55.0%
CAAGCATGGAGACCTCAGAG+AGG - chr3.2:89569785-89569804 MS.gene42308:intron 55.0%
CACTGCCTGCAGGATTCACA+AGG - chr3.2:89568933-89568952 MS.gene42308:CDS 55.0%
GGCAGTGATCTGAGGAGTGA+GGG + chr3.2:89568920-89568939 None:intergenic 55.0%
GGTACGTGGACCAACAGCTT+AGG - chr3.2:89569726-89569745 MS.gene42308:CDS 55.0%
CTCCTCAGATCACTGCCTGC+AGG - chr3.2:89568923-89568942 MS.gene42308:CDS 60.0%
TTGGACATGGTTGCTGGGGG+TGG - chr3.2:89569569-89569588 MS.gene42308:CDS 60.0%
TGGTCCACGTACCGGAGACG+AGG + chr3.2:89569719-89569738 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 89568483 89570829 89568483 ID=MS.gene42308
chr3.2 mRNA 89568483 89570829 89568483 ID=MS.gene42308.t1;Parent=MS.gene42308
chr3.2 exon 89570071 89570829 89570071 ID=MS.gene42308.t1.exon1;Parent=MS.gene42308.t1
chr3.2 CDS 89570071 89570829 89570071 ID=cds.MS.gene42308.t1;Parent=MS.gene42308.t1
chr3.2 exon 89569445 89569797 89569445 ID=MS.gene42308.t1.exon2;Parent=MS.gene42308.t1
chr3.2 CDS 89569445 89569797 89569445 ID=cds.MS.gene42308.t1;Parent=MS.gene42308.t1
chr3.2 exon 89569216 89569276 89569216 ID=MS.gene42308.t1.exon3;Parent=MS.gene42308.t1
chr3.2 CDS 89569216 89569276 89569216 ID=cds.MS.gene42308.t1;Parent=MS.gene42308.t1
chr3.2 exon 89568483 89569088 89568483 ID=MS.gene42308.t1.exon4;Parent=MS.gene42308.t1
chr3.2 CDS 89568483 89569088 89568483 ID=cds.MS.gene42308.t1;Parent=MS.gene42308.t1
Gene Sequence

>MS.gene42308

ATGTATCCCAACCAAGCATTCTCTTCAGGGTCCTATATGGATATGTTCACCTCGAGTCCTTTGTTACCTCACAATTACTACAATGAATCTGTAGCAGAACAAAATGAAACCAAGTTTAACACACCTATAGGCGACACAGTGACTATGCAGTCCATTGATGAGCATTCCAATGCTTACAGCTCTCATGTAGATGAGCAAAATATACAATGCCAAGAGTTATCTCTTAGTCTCGGGACACTACTGCCTTCTAATGCATCTGTCCCTCCATTTCAATACCAGTATAATGACACCGGTTTCGTATCGCTGATGAATTCTTGTCGTCTTCCAAAGGGAACTACTATATCTAACGAAGATGAATTGAAAAGTGCCGAATGCATGGAAAGCATGTCTTCCGGAGGATTTCATGACATTACCAAAAGGGAGTGTTTTCGCAACCCTCACTCCTCAGATCACTGCCTGCAGGATTCACAAGGTTTTTCCAACATTTTCCTAAACTCTCAATATCTTAAGGCTACACAGGAGTTACTTGATGAGATAGTTAATGTCCGAAAGCAACATGGAATGGAAAAGCAAGAAAAAATCCGCAACATTGGATTGGATAATCCCAAAGATAGTGATGGAAAATCTACTAGTCAGTCTATGCAAATAACTTCGGGGGGACCTAATGATTCTAATTCCAATCCTTCTTGTGAGATATCCTCAGCCGAAAGACAGAATTTGTTGGATAAGAAGACAAAGCTTTTGTCCATGTTGGATGAGGTAACCATATTATTTAGCATTGCATCCTTCCTTTTATGGATTTAATTTGTATGCTCTAACAGACTAACAGTGCAAAAACTTTATCATAAGGTTCTATCATCGAAAAAGTTTGACTATTATCATATCTAACTTTTATTGGTTAATAGTGTAAAATAATTTTATACCGATAGTGTGTGACAATTATTCTCTTCTTTTATTCCATAGTTGGTTATTATGACAGCGATGGTTTGGCTGATTGTCGAATCTGTAATCCTTTCTTTGTACTGGTTGTAGGTAGGCAAAAGATACAGACAGTACTGCCATCGAATGCAAATTGTGGTGTCATCTTTGGACATGGTTGCTGGGGGTGGAGCAGCAGAGCCATATACTACACTTGCATTGCGAACAGTTTCGCGTCAGTTTCGCTGCTTGCGCGATGCTATCAGCAACCAAGTTCAAGTGATTCAAAGGAACCTTGGGGAACAAGAAGGAATACCTCGTCTCCGGTACGTGGACCAACAGCTTAGGCAACAAAAGGCACTTCAGCATCTTGGTGTTATGCGGCAAGCATGGAGACCTCAGAGAGGACTTCCCGAAAACTCTGTTTCAATACTTCGTGCTTGGCTATTTGAACATTTTCTTAATCCGTATGTGTTACTTTATAATACCCTCTAAATTTCTAATCCTTCACTCAATTTTATATTTTCAATTAGTTGCAATGGTTTCATATTATGCTACTTTCATAATATTTTTTATCTATGTGTATTTTAATCAAATTCTTTTTCAACTTTCATTTCATAATTGGTTGATACCAGTTACCCCAAGGATTCAGAGAAAATAATGCTAGCTAGGCAAACTGGTTTGACAAGGAATCAGGTACAGTTATAATTAATCAAATAATTTTTTATGTGCAACATTATTTTCATTAAACTTGTTGCATTGCATAATCAGGTACAGTTACCAATTGTGGTGTCATCTTTTAATTTATATGGTATGGTATCAGGTGGCAAATTGGTTCATTAATGCACGTGTGCGTCTTTGGAAACCAATGGTTGAGGAAATATACAAAGAAGAGTTTGGTGATTCTGAAATGAACTGCAATTTTTCATCTGAAAATACACTCCAAGGTAAAAGAGATTATGGTCAAGAGATTAATAGTAATAAATTGGAAGAAGAGTCACATGACAATGTACAAATTACTTGTGATCATAGTGTACAAGCCTCTTCCACTAGACAACATGTAGAAAAAAATGTGATAGATTGTGGAAATGGAAAATTGCATGGCAACCAAAGGCTCAGCATAAAAAATGGCTTTTATTCACAAATAGATTCTGATTCTGCCACATATGATTTAACAGAGTTGGGTAACTTTACAACTGTGGATGGTGGTGGCCATCACAATGTCTCTCTTGCATTGGAGTTGAGAAACTGTGAAAGCGATGGATTAGCCACATCTGATGATGCCATTCATAAAAGACATAATAATAATAATCAGACATTGGTTTCTTCTTCACAGAGTACTGATTTACTAGACTATCATTTCACAGATTCAGGAAAGCAACAACACATGTTTGGCAATACTCATCTGTTGCATGAATTTGTCGTATGA
Protein sequence

>MS.gene42308.t1

MYPNQAFSSGSYMDMFTSSPLLPHNYYNESVAEQNETKFNTPIGDTVTMQSIDEHSNAYSSHVDEQNIQCQELSLSLGTLLPSNASVPPFQYQYNDTGFVSLMNSCRLPKGTTISNEDELKSAECMESMSSGGFHDITKRECFRNPHSSDHCLQDSQGFSNIFLNSQYLKATQELLDEIVNVRKQHGMEKQEKIRNIGLDNPKDSDGKSTSQSMQITSGGPNDSNSNPSCEISSAERQNLLDKKTKLLSMLDEVGKRYRQYCHRMQIVVSSLDMVAGGGAAEPYTTLALRTVSRQFRCLRDAISNQVQVIQRNLGEQEGIPRLRYVDQQLRQQKALQHLGVMRQAWRPQRGLPENSVSILRAWLFEHFLNPYPKDSEKIMLARQTGLTRNQVANWFINARVRLWKPMVEEIYKEEFGDSEMNCNFSSENTLQGKRDYGQEINSNKLEEESHDNVQITCDHSVQASSTRQHVEKNVIDCGNGKLHGNQRLSIKNGFYSQIDSDSATYDLTELGNFTTVDGGGHHNVSLALELRNCESDGLATSDDAIHKRHNNNNQTLVSSSQSTDLLDYHFTDSGKQQHMFGNTHLLHEFVV