AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42755

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42755.t1 MTR_6g444980 65.464 388 78 18 1 345 1 375 1.30e-147 421
MS.gene42755.t1 MTR_6g444980 60.677 384 76 18 1 345 1 348 3.45e-129 373
MS.gene42755.t1 MTR_5g027440 45.798 238 92 11 13 219 1 232 1.05e-51 173
MS.gene42755.t1 MTR_2g086450 52.941 170 63 2 26 178 35 204 9.44e-51 174
MS.gene42755.t1 MTR_4g081710 55.195 154 61 1 29 174 10 163 1.71e-50 171
MS.gene42755.t1 MTR_6g032990 46.190 210 91 7 29 221 35 239 4.57e-50 172
MS.gene42755.t1 MTR_5g041350 55.414 157 61 2 29 176 8 164 3.97e-49 168
MS.gene42755.t1 MTR_2g027800 53.529 170 67 4 24 183 27 194 1.26e-46 160
MS.gene42755.t1 MTR_1g090670 52.381 168 67 4 15 173 23 186 2.11e-45 157
MS.gene42755.t1 MTR_7g088070 54.545 154 54 5 36 182 50 194 1.10e-44 154
MS.gene42755.t1 MTR_2g027860 59.398 133 51 2 36 167 49 179 8.83e-44 154
MS.gene42755.t1 MTR_7g088070 53.247 154 52 4 36 182 50 190 2.61e-43 151
MS.gene42755.t1 MTR_2g027800 47.191 178 52 4 24 183 27 180 1.27e-40 144
MS.gene42755.t1 MTR_7g098250 38.164 207 109 6 36 236 262 455 3.54e-36 137
MS.gene42755.t1 MTR_1g080330 40.237 169 85 4 2 167 148 303 6.29e-34 129
MS.gene42755.t1 MTR_7g115530 41.722 151 77 3 36 183 218 360 7.78e-34 129
MS.gene42755.t1 MTR_1g053835 42.657 143 71 3 32 171 254 388 6.31e-30 119
MS.gene42755.t1 MTR_7g093030 40.714 140 75 3 36 173 212 345 6.29e-29 115
MS.gene42755.t1 MTR_1g080330 39.865 148 73 4 2 146 148 282 1.97e-26 106
MS.gene42755.t1 MTR_7g115530 44.737 114 54 2 36 146 218 325 3.98e-26 106
MS.gene42755.t1 MTR_7g068600 43.182 132 69 2 36 164 13 141 2.93e-25 102
MS.gene42755.t1 MTR_1g053800 39.103 156 83 2 26 177 167 314 5.10e-25 103
MS.gene42755.t1 MTR_7g115530 63.158 57 21 0 36 92 218 274 8.63e-20 88.6
MS.gene42755.t1 MTR_7g115530 63.158 57 21 0 36 92 218 274 3.42e-19 87.8
MS.gene42755.t1 MTR_1g090670 46.087 115 49 4 68 173 1 111 4.49e-19 85.1
MS.gene42755.t1 MTR_7g115530 66.038 53 18 0 36 88 218 270 3.29e-18 83.6
MS.gene42755.t1 MTR_7g093030 57.895 57 24 0 36 92 212 268 5.61e-18 83.6
MS.gene42755.t1 MTR_7g115530 66.667 51 17 0 36 86 218 268 2.85e-17 81.3
MS.gene42755.t1 MTR_2g023580 50.000 78 37 1 37 112 56 133 8.54e-17 79.0
MS.gene42755.t1 MTR_3g450310 36.364 132 62 3 36 158 19 137 3.92e-16 76.6
MS.gene42755.t1 MTR_7g069660 55.357 56 25 0 37 92 72 127 1.69e-15 77.0
MS.gene42755.t1 MTR_3g064840 51.429 70 34 0 37 106 275 344 2.00e-15 77.4
MS.gene42755.t1 MTR_6g071625 56.667 60 26 0 37 96 203 262 2.23e-15 76.6
MS.gene42755.t1 MTR_1g112370 56.452 62 22 1 37 93 23 84 2.72e-15 76.3
MS.gene42755.t1 MTR_0223s0040 62.264 53 20 0 37 89 19 71 3.79e-15 75.1
MS.gene42755.t1 MTR_8g066310 58.929 56 23 0 37 92 174 229 6.25e-15 75.5
MS.gene42755.t1 MTR_5g029470 58.929 56 23 0 37 92 220 275 7.82e-15 75.5
MS.gene42755.t1 MTR_1g053830 49.091 55 28 0 38 92 20 74 1.07e-14 69.7
MS.gene42755.t1 MTR_1g021520 57.143 56 24 0 37 92 127 182 1.62e-14 73.6
MS.gene42755.t1 MTR_5g041350 71.739 46 13 0 131 176 8 53 1.88e-14 72.4
MS.gene42755.t1 MTR_8g086410 53.571 56 26 0 37 92 112 167 2.23e-14 73.9
MS.gene42755.t1 MTR_8g086410 53.571 56 26 0 37 92 110 165 2.25e-14 73.9
MS.gene42755.t1 MTR_5g041350 71.739 46 13 0 131 176 30 75 3.20e-14 72.0
MS.gene42755.t1 MTR_2g016220 55.357 56 25 0 37 92 90 145 3.25e-14 73.2
MS.gene42755.t1 MTR_2g016220 55.357 56 25 0 37 92 90 145 3.33e-14 73.2
MS.gene42755.t1 MTR_2g084230 55.357 56 25 0 37 92 98 153 4.11e-14 72.4
MS.gene42755.t1 MTR_2g084230 55.357 56 25 0 37 92 98 153 4.16e-14 73.2
MS.gene42755.t1 MTR_7g093010 37.500 128 68 4 36 158 213 333 4.43e-14 72.8
MS.gene42755.t1 MTR_4g121020 59.322 59 23 1 32 90 107 164 1.15e-13 72.4
MS.gene42755.t1 MTR_4g121020 59.322 59 23 1 32 90 211 268 1.39e-13 72.0
MS.gene42755.t1 MTR_4g121020 59.322 59 23 1 32 90 211 268 1.39e-13 72.0
MS.gene42755.t1 MTR_4g064730 53.623 69 30 2 23 90 117 184 2.82e-13 70.1
MS.gene42755.t1 MTR_8g086410 56.000 50 22 0 37 86 112 161 3.20e-13 70.5
MS.gene42755.t1 MTR_1g032570 61.818 55 20 1 36 90 131 184 3.46e-13 70.9
MS.gene42755.t1 MTR_2g016220 58.000 50 21 0 37 86 90 139 3.84e-13 70.1
MS.gene42755.t1 MTR_1g032570 61.818 55 20 1 36 90 200 253 4.29e-13 70.5
MS.gene42755.t1 MTR_7g089010 61.818 55 20 1 36 90 91 144 5.46e-13 68.9
MS.gene42755.t1 MTR_1g093080 60.000 55 22 0 36 90 211 265 9.54e-13 68.9
MS.gene42755.t1 MTR_2g034960 54.237 59 26 1 32 90 207 264 2.02e-12 68.6
MS.gene42755.t1 MTR_5g017980 60.000 55 22 0 36 90 193 247 2.50e-12 67.4
MS.gene42755.t1 MTR_5g054300 58.182 55 23 0 36 90 198 252 9.81e-12 65.9
MS.gene42755.t1 MTR_4g021790 52.542 59 27 1 32 90 164 221 1.01e-11 65.1
MS.gene42755.t1 MTR_4g086835 55.172 58 26 0 33 90 271 328 3.97e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42755.t1 AT1G79430 54.247 365 137 9 4 345 1 358 9.88e-115 337
MS.gene42755.t1 AT1G79430 47.333 300 129 8 68 345 1 293 7.41e-71 223
MS.gene42755.t1 AT3G12730 61.453 179 60 3 25 194 12 190 3.32e-67 211
MS.gene42755.t1 AT4G13640 46.097 269 106 6 26 269 27 281 4.57e-64 205
MS.gene42755.t1 AT3G24120 68.790 157 32 4 25 168 30 182 7.69e-63 202
MS.gene42755.t1 AT4G13640 45.588 272 106 7 26 269 27 284 5.45e-62 200
MS.gene42755.t1 AT3G24120 67.500 160 32 5 25 168 30 185 1.19e-60 197
MS.gene42755.t1 AT5G18240 54.082 196 70 6 25 207 34 222 3.27e-56 188
MS.gene42755.t1 AT5G18240 54.082 196 70 6 25 207 34 222 3.27e-56 188
MS.gene42755.t1 AT3G04030 58.580 169 58 3 25 181 34 202 1.06e-55 187
MS.gene42755.t1 AT5G18240 53.061 196 70 7 25 207 34 220 8.85e-53 179
MS.gene42755.t1 AT3G04030 57.396 169 59 4 25 181 34 201 1.46e-52 178
MS.gene42755.t1 AT5G18240 51.531 196 69 7 25 207 34 216 1.84e-49 170
MS.gene42755.t1 AT5G18240 51.531 196 69 7 25 207 34 216 1.84e-49 170
MS.gene42755.t1 AT3G04030 55.621 169 57 4 25 181 34 196 4.47e-49 169
MS.gene42755.t1 AT1G69580 49.412 170 72 2 29 184 27 196 1.44e-47 164
MS.gene42755.t1 AT1G69580 49.123 171 72 2 29 184 27 197 2.17e-47 163
MS.gene42755.t1 AT5G45580 41.629 221 103 6 26 220 13 233 1.11e-45 157
MS.gene42755.t1 AT5G45580 41.935 217 100 6 30 220 1 217 9.39e-45 154
MS.gene42755.t1 AT2G01060 58.333 132 53 1 36 167 15 144 3.38e-44 154
MS.gene42755.t1 AT3G12730 53.676 136 54 3 68 194 1 136 4.70e-37 132
MS.gene42755.t1 AT4G28610 39.881 168 91 3 36 199 225 386 7.71e-34 129
MS.gene42755.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene42755.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene42755.t1 AT3G13040 44.172 163 76 5 22 171 218 378 9.76e-34 129
MS.gene42755.t1 AT5G29000 41.290 155 88 2 13 167 168 319 9.13e-32 122
MS.gene42755.t1 AT5G29000 41.290 155 88 2 13 167 168 319 9.13e-32 122
MS.gene42755.t1 AT5G29000 41.290 155 88 2 13 167 211 362 1.56e-31 123
MS.gene42755.t1 AT5G06800 45.522 134 70 2 36 167 192 324 3.96e-30 118
MS.gene42755.t1 AT3G04450 42.963 135 71 2 36 167 198 329 4.04e-30 119
MS.gene42755.t1 AT3G04450 42.963 135 71 2 36 167 198 329 4.04e-30 119
MS.gene42755.t1 AT5G06800 46.617 133 65 3 36 167 192 319 4.87e-30 118
MS.gene42755.t1 AT3G04450 42.963 135 71 2 36 167 238 369 5.10e-30 119
MS.gene42755.t1 AT3G04030 52.381 126 48 3 68 181 1 126 1.43e-28 113
MS.gene42755.t1 AT2G20400 39.103 156 93 1 36 191 231 384 1.23e-27 112
MS.gene42755.t1 AT2G20400 38.365 159 89 2 36 191 231 383 1.29e-27 112
MS.gene42755.t1 AT5G18240 43.791 153 60 7 68 207 1 140 5.74e-23 97.8
MS.gene42755.t1 AT3G04030 48.413 126 47 4 68 181 1 120 2.53e-22 95.9
MS.gene42755.t1 AT2G01060 49.485 97 47 1 71 167 1 95 4.52e-21 90.9
MS.gene42755.t1 AT2G06020 59.375 64 26 0 29 92 79 142 7.01e-21 91.7
MS.gene42755.t1 AT5G06800 59.649 57 23 0 36 92 192 248 5.36e-18 83.2
MS.gene42755.t1 AT5G29000 48.750 80 38 2 13 92 211 287 1.91e-17 82.0
MS.gene42755.t1 AT3G04450 56.140 57 25 0 36 92 238 294 3.45e-17 81.6
MS.gene42755.t1 AT1G14600 50.685 73 31 1 22 89 4 76 1.75e-16 78.6
MS.gene42755.t1 AT2G40260 60.714 56 22 0 37 92 83 138 4.35e-16 79.3
MS.gene42755.t1 AT2G42660 58.929 56 23 0 37 92 51 106 5.75e-16 77.0
MS.gene42755.t1 AT4G04605 58.491 53 22 0 37 89 15 67 6.43e-16 76.3
MS.gene42755.t1 AT2G02060 57.627 59 25 0 35 93 28 86 1.50e-15 75.9
MS.gene42755.t1 AT2G02060 57.627 59 25 0 35 93 28 86 1.50e-15 75.9
MS.gene42755.t1 AT1G32240 56.667 60 26 0 37 96 214 273 5.11e-15 75.9
MS.gene42755.t1 AT5G16560 56.667 60 26 0 37 96 220 279 5.53e-15 75.9
MS.gene42755.t1 AT1G32240 58.929 56 23 0 37 92 214 269 6.50e-15 75.5
MS.gene42755.t1 AT2G38300 57.143 56 24 0 37 92 55 110 7.38e-15 75.1
MS.gene42755.t1 AT5G42630 57.143 56 24 0 37 92 106 161 1.36e-14 72.4
MS.gene42755.t1 AT5G42630 57.143 56 24 0 37 92 106 161 2.06e-14 72.8
MS.gene42755.t1 AT4G17695 42.857 84 45 1 37 117 165 248 2.44e-14 73.2
MS.gene42755.t1 AT3G46640 55.714 70 27 2 24 90 129 197 4.29e-14 72.8
MS.gene42755.t1 AT3G46640 55.714 70 27 2 24 90 129 197 4.29e-14 72.8
MS.gene42755.t1 AT3G46640 55.714 70 27 2 24 90 129 197 5.52e-14 72.4
MS.gene42755.t1 AT5G59570 54.286 70 28 2 24 90 126 194 8.76e-14 71.2
MS.gene42755.t1 AT5G59570 54.286 70 28 2 24 90 126 194 8.76e-14 71.2
MS.gene42755.t1 AT5G05090 61.818 55 20 1 36 90 81 134 1.66e-13 70.1
MS.gene42755.t1 AT3G10760 61.818 55 20 1 36 90 105 158 2.55e-13 70.5
MS.gene42755.t1 AT4G04580 47.059 68 33 1 37 104 15 79 4.69e-13 67.0
MS.gene42755.t1 AT2G40970 60.000 55 21 1 36 90 105 158 1.03e-12 67.8
MS.gene42755.t1 AT4G37180 55.172 58 26 0 38 95 212 269 1.04e-12 68.9
MS.gene42755.t1 AT4G37180 55.172 58 26 0 38 95 219 276 1.12e-12 68.6
MS.gene42755.t1 AT5G58080 31.481 162 99 5 33 190 191 344 1.43e-12 68.9
MS.gene42755.t1 AT3G25790 60.000 50 20 0 41 90 201 250 4.30e-12 67.0
MS.gene42755.t1 AT3G16857 56.364 55 23 1 36 90 167 220 9.56e-12 66.6
MS.gene42755.t1 AT1G49560 52.941 68 31 1 36 102 192 259 1.02e-11 65.5
MS.gene42755.t1 AT3G16857 56.364 55 23 1 36 90 237 290 1.13e-11 66.2
MS.gene42755.t1 AT3G16857 56.364 55 23 1 36 90 237 290 1.20e-11 66.2
MS.gene42755.t1 AT2G01760 54.545 55 24 1 36 90 135 188 2.19e-11 64.3
MS.gene42755.t1 AT5G44190 55.556 54 23 1 36 89 147 199 5.18e-11 63.9
MS.gene42755.t1 AT5G44190 55.556 54 23 1 36 89 147 199 6.44e-11 63.2
MS.gene42755.t1 AT1G13300 54.545 55 25 0 36 90 181 235 6.75e-11 63.2

Find 81 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCCACTTCCATTAAA+AGG 0.118507 8.2:-54955278 None:intergenic
TCATTATCATTCCAAATTAT+TGG 0.120463 8.2:-54955302 None:intergenic
AATGATTCACCTTTGAAATT+AGG 0.181239 8.2:-54955380 None:intergenic
TGATCTTAGGCTTCATGATT+TGG 0.243321 8.2:+54955322 MS.gene42755:CDS
ATCATAGTTGAGGGCTTCTT+TGG 0.252047 8.2:-54952912 None:intergenic
GTGGAAAGAGTCCAATAATT+TGG 0.261771 8.2:+54955291 MS.gene42755:CDS
TTTGGAATGATAATGATCTT+AGG 0.294293 8.2:+54955309 MS.gene42755:CDS
AAAATACATGCAAAGTATAT+TGG 0.312337 8.2:+54954986 MS.gene42755:CDS
AATGATGAAAATTTGTTTGT+TGG 0.313875 8.2:+54955239 MS.gene42755:CDS
ATTCAATGAGCAATCAATTA+AGG 0.320363 8.2:+54953633 MS.gene42755:CDS
CAGCATCAACAAAGCGTTCA+TGG 0.328267 8.2:-54953028 None:intergenic
CCACCTTAAGAGCCATCTTC+AGG 0.346223 8.2:+54953442 MS.gene42755:CDS
GATCTTAGGCTTCATGATTT+GGG 0.360986 8.2:+54955323 MS.gene42755:CDS
TTTGAACTGCAAAGAAATAC+TGG 0.365692 8.2:+54953785 MS.gene42755:CDS
TAATTTCAAAGGTGAATCAT+TGG 0.367186 8.2:+54955382 MS.gene42755:CDS
GTCCATCGAAGGCGTGGTTT+CGG 0.373372 8.2:-54952996 None:intergenic
AAAGCTTATCACACACTTGC+AGG 0.378431 8.2:+54955011 MS.gene42755:CDS
ATAGAGAGGCCAACCCTTGA+TGG 0.379955 8.2:+54955194 MS.gene42755:CDS
GAAACGAATTCTTTCATGTC+AGG 0.392772 8.2:-54955092 None:intergenic
AAAACAATCATGAGGGTTAT+GGG 0.394376 8.2:+54953398 MS.gene42755:CDS
GTTGATGCTGTTGCTCAGCT+TGG 0.397802 8.2:+54953041 MS.gene42755:CDS
AAACCTGAAGATGGCTCTTA+AGG 0.399926 8.2:-54953445 None:intergenic
TTTCAAGATCTTAACATTTG+TGG 0.407965 8.2:+54955137 MS.gene42755:CDS
ACAAATTTCAAGGGTATGAA+TGG 0.427658 8.2:+54955056 MS.gene42755:CDS
ATTTGTTTGTTGGAAAGAAA+AGG 0.433652 8.2:+54955249 MS.gene42755:CDS
GAGCCTCAATCCTCAATTGA+AGG 0.444605 8.2:-54954959 None:intergenic
TTGCTCATTGAATTCCTTGT+GGG 0.445168 8.2:-54953623 None:intergenic
AAATGAGGAAAATTATTAAG+AGG 0.450258 8.2:-54955116 None:intergenic
TTTGATCCATCTGTGAAGCT+TGG 0.458634 8.2:+54955467 MS.gene42755:CDS
GGTAGAAGCTCATCACCATT+TGG 0.458779 8.2:+54955524 MS.gene42755:CDS
TTCAAAAGCACCTTCAATTG+AGG 0.460223 8.2:+54954949 MS.gene42755:CDS
GCTGCAACAGCATTGATCAT+AGG 0.461064 8.2:-54955500 None:intergenic
GATGCTGTTGCTCAGCTTGG+AGG 0.468617 8.2:+54953044 MS.gene42755:CDS
AATTAGTATTCTCAACCAAA+TGG 0.471739 8.2:-54955539 None:intergenic
TCTTTCCACTTCCATTAAAA+GGG 0.471840 8.2:-54955277 None:intergenic
AGCAGCAGCAACAAATTTCA+AGG 0.477460 8.2:+54955046 MS.gene42755:CDS
GATGGCTCTTAAGGTGGTAA+AGG 0.478682 8.2:-54953436 None:intergenic
TTGAAATTAGGAACATCATC+AGG 0.480806 8.2:-54955368 None:intergenic
TGAGAATTCATCATAGTTGA+GGG 0.480954 8.2:-54952921 None:intergenic
ATAGTTGAGGGCTTCTTTGG+AGG 0.483070 8.2:-54952909 None:intergenic
TAGGCATTATAATTCCATCA+AGG 0.485394 8.2:-54955208 None:intergenic
ATGTGTGTTCAAGGTGACTC+TGG 0.488847 8.2:+54952954 MS.gene42755:CDS
GATAGACCCATGTGTGTTCA+AGG 0.494833 8.2:+54952945 MS.gene42755:CDS
CATTAATAGAAATTTAGGCT+TGG 0.499746 8.2:+54953592 MS.gene42755:intron
AGGCATTATAATTCCATCAA+GGG 0.502907 8.2:-54955207 None:intergenic
TAGTTGAGGGCTTCTTTGGA+GGG 0.504455 8.2:-54952908 None:intergenic
ATGGCTCTTAAGGTGGTAAA+GGG 0.506688 8.2:-54953435 None:intergenic
GCACCTTCAATTGAGGATTG+AGG 0.506889 8.2:+54954956 MS.gene42755:CDS
GATGATGTTCCTAATTTCAA+AGG 0.507643 8.2:+54955371 MS.gene42755:CDS
ATTATAATTCCATCAAGGGT+TGG 0.522631 8.2:-54955203 None:intergenic
ATCCGAAACCACGCCTTCGA+TGG 0.524006 8.2:+54952994 MS.gene42755:CDS
ATGATCAATGCTGTTGCAGC+TGG 0.528955 8.2:+54955503 MS.gene42755:CDS
GGCACCTCGTCTTCTATGAC+TGG 0.530737 8.2:+54953806 MS.gene42755:CDS
CACACTTGCAGGTGAAAACA+TGG 0.531497 8.2:+54955022 MS.gene42755:CDS
ATGTTAAGATCTTGAAAATG+AGG 0.534145 8.2:-54955131 None:intergenic
ATGAGAATTCATCATAGTTG+AGG 0.541419 8.2:-54952922 None:intergenic
CATGAGGGTTATGGGTGTGA+AGG 0.546666 8.2:+54953406 MS.gene42755:CDS
TGTGTGTTCAAACCTGAAGA+TGG 0.568193 8.2:-54953454 None:intergenic
GTTATTCCAAGCTTCACAGA+TGG 0.570892 8.2:-54955473 None:intergenic
AAACGAATTCTTTCATGTCA+GGG 0.571485 8.2:-54955091 None:intergenic
TCGGATCAGTGGTGAGGACA+AGG 0.575673 8.2:-54952977 None:intergenic
GGAGTTCGACGGTCCATCGA+AGG 0.577331 8.2:-54953007 None:intergenic
TCGACGGTCCATCGAAGGCG+TGG 0.581352 8.2:-54953002 None:intergenic
ATGAGGGTTATGGGTGTGAA+GGG 0.590465 8.2:+54953407 MS.gene42755:CDS
ATTGCTCATTGAATTCCTTG+TGG 0.591176 8.2:-54953624 None:intergenic
CCTGAAGATGGCTCTTAAGG+TGG 0.594196 8.2:-54953442 None:intergenic
TGCTCATTGAATTCCTTGTG+GGG 0.596962 8.2:-54953622 None:intergenic
AAGGGTATGAATGGAACTCA+AGG 0.599882 8.2:+54955065 MS.gene42755:CDS
AATGAGCAATCAATTAAGGA+TGG 0.599964 8.2:+54953637 MS.gene42755:CDS
AGAGTCACCTTGAACACACA+TGG 0.606753 8.2:-54952952 None:intergenic
ATCTTCATCAAAACATAGAG+AGG 0.609398 8.2:+54955180 MS.gene42755:CDS
AAGGCGTGGTTTCGGATCAG+TGG 0.610084 8.2:-54952988 None:intergenic
TCAATTAAGGATGGTATGAG+AGG 0.611305 8.2:+54953646 MS.gene42755:CDS
GCTTGGAAAGCAACCCCACA+AGG 0.615422 8.2:+54953609 MS.gene42755:CDS
CAATTGAGGATTGAGGCTCA+AGG 0.648335 8.2:+54954963 MS.gene42755:CDS
GCAGCAGCAACAAATTTCAA+GGG 0.652422 8.2:+54955047 MS.gene42755:CDS
GTGGTTTCGGATCAGTGGTG+AGG 0.654708 8.2:-54952983 None:intergenic
AAGACTGCATGAACAGCTAG+AGG 0.664015 8.2:+54954297 MS.gene42755:CDS
AAAGCGTTCATGGAGTTCGA+CGG 0.684910 8.2:-54953018 None:intergenic
GCGACCAGTCATAGAAGACG+AGG 0.697663 8.2:-54953810 None:intergenic
GAGTCACCTTGAACACACAT+GGG 0.714712 8.2:-54952951 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTAATTTTTTTATTATT+AGG - chr8.2:54954421-54954440 None:intergenic 0.0%
!!! ATTAAATTAATTTAATATTT+TGG + chr8.2:54954433-54954452 MS.gene42755:intron 0.0%
!! TAAATAAATTAAGAATCAAA+AGG - chr8.2:54953862-54953881 None:intergenic 10.0%
!! TTAAGACAAAAAAATATATA+TGG + chr8.2:54954881-54954900 MS.gene42755:intron 10.0%
!!! TTTTCATCATTATTATTATT+AGG - chr8.2:54955230-54955249 None:intergenic 10.0%
!! AAAAGAAAGAAAAGAATAAA+AGG - chr8.2:54953091-54953110 None:intergenic 15.0%
!! ATAAAATCAAAGAACTTAAA+AGG - chr8.2:54953563-54953582 None:intergenic 15.0%
!! ATTATTAGGAAAAAAAAAGT+CGG - chr8.2:54954407-54954426 None:intergenic 15.0%
!! TAAAATCAAAGAACTTAAAA+GGG - chr8.2:54953562-54953581 None:intergenic 15.0%
!!! CAATTTTTTTAGACATTTTT+AGG - chr8.2:54954579-54954598 None:intergenic 15.0%
!!! TTTTATTCTTTTCTTTCTTT+TGG + chr8.2:54953090-54953109 MS.gene42755:intron 15.0%
!!! TTTTGCATTAATAGAAATTT+AGG + chr8.2:54953587-54953606 MS.gene42755:intron 15.0%
!!! TTTTTGTTTTTGTTTTTTGA+GGG - chr8.2:54953237-54953256 None:intergenic 15.0%
!!! TTTTTTGTTTTTGTTTTTTG+AGG - chr8.2:54953238-54953257 None:intergenic 15.0%
!! AAAATACATGCAAAGTATAT+TGG + chr8.2:54954986-54955005 MS.gene42755:CDS 20.0%
!! AAATGAGGAAAATTATTAAG+AGG - chr8.2:54955119-54955138 None:intergenic 20.0%
!! AGTTACGATAAATACTTATT+TGG - chr8.2:54954510-54954529 None:intergenic 20.0%
!! AGTTATTAGTTATTATATGC+AGG - chr8.2:54954049-54954068 None:intergenic 20.0%
!! CATATTATAATGAGAAACTT+TGG + chr8.2:54954173-54954192 MS.gene42755:intron 20.0%
!! TCATTATCATTCCAAATTAT+TGG - chr8.2:54955305-54955324 None:intergenic 20.0%
!! TTGGTTAATAAACTTCAATA+AGG + chr8.2:54954735-54954754 MS.gene42755:intron 20.0%
!!! AATGATGAAAATTTGTTTGT+TGG + chr8.2:54955239-54955258 MS.gene42755:CDS 20.0%
!!! GTAACTATATTATTGAGTTT+TGG + chr8.2:54954524-54954543 MS.gene42755:intron 20.0%
!!! TTAATTTGTAGGTTTATATG+TGG + chr8.2:54954922-54954941 MS.gene42755:intron 20.0%
!!! TTTCAATATTAGTTTACTTG+TGG - chr8.2:54953898-54953917 None:intergenic 20.0%
!!! TTTTAAGAAAGATTTGAACT+CGG - chr8.2:54954781-54954800 None:intergenic 20.0%
! AATAATTATTCTTGGGGATT+TGG + chr8.2:54954134-54954153 MS.gene42755:intron 25.0%
! AATATATATGGTGCAAATGT+TGG + chr8.2:54954893-54954912 MS.gene42755:intron 25.0%
! ATGTTAAGATCTTGAAAATG+AGG - chr8.2:54955134-54955153 None:intergenic 25.0%
! ATTCAATGAGCAATCAATTA+AGG + chr8.2:54953633-54953652 MS.gene42755:CDS 25.0%
! CATTAATAGAAATTTAGGCT+TGG + chr8.2:54953592-54953611 MS.gene42755:intron 25.0%
! CTAAATGAGGTAAAGAAATT+TGG - chr8.2:54953496-54953515 None:intergenic 25.0%
! GTGTCTAAAAACTATACATT+TGG + chr8.2:54954476-54954495 MS.gene42755:intron 25.0%
! GTTAATAAACTTCAATAAGG+TGG + chr8.2:54954738-54954757 MS.gene42755:intron 25.0%
! TAATTTCAAAGGTGAATCAT+TGG + chr8.2:54955382-54955401 MS.gene42755:CDS 25.0%
! TGCTCACAATAATTATTCTT+GGG + chr8.2:54954127-54954146 MS.gene42755:intron 25.0%
! TTTCAAGATCTTAACATTTG+TGG + chr8.2:54955137-54955156 MS.gene42755:CDS 25.0%
!! AAAGCTAGTTTCTAATTTCT+AGG + chr8.2:54954618-54954637 MS.gene42755:intron 25.0%
!! ATTTGTTTGTTGGAAAGAAA+AGG + chr8.2:54955249-54955268 MS.gene42755:CDS 25.0%
!! TTTGGAATGATAATGATCTT+AGG + chr8.2:54955309-54955328 MS.gene42755:CDS 25.0%
!!! AATGATTCACCTTTGAAATT+AGG - chr8.2:54955383-54955402 None:intergenic 25.0%
!!! TTTTGTTTTCATGACAGTTT+CGG + chr8.2:54953760-54953779 MS.gene42755:intron 25.0%
AAAAACAATCATGAGGGTTA+TGG + chr8.2:54953397-54953416 MS.gene42755:CDS 30.0%
AAAACAATCATGAGGGTTAT+GGG + chr8.2:54953398-54953417 MS.gene42755:CDS 30.0%
AAACGAATTCTTTCATGTCA+GGG - chr8.2:54955094-54955113 None:intergenic 30.0%
AAATAACAAAGCGATTGAGT+TGG + chr8.2:54954716-54954735 MS.gene42755:intron 30.0%
AACCGTAACTAAAACATTGT+TGG + chr8.2:54954240-54954259 MS.gene42755:intron 30.0%
AATGAGCAATCAATTAAGGA+TGG + chr8.2:54953637-54953656 MS.gene42755:CDS 30.0%
AGACGATATGTATAGTGTTT+AGG + chr8.2:54954373-54954392 MS.gene42755:intron 30.0%
AGGCATTATAATTCCATCAA+GGG - chr8.2:54955210-54955229 None:intergenic 30.0%
ATCTTCATCAAAACATAGAG+AGG + chr8.2:54955180-54955199 MS.gene42755:CDS 30.0%
ATGAGAATTCATCATAGTTG+AGG - chr8.2:54952925-54952944 None:intergenic 30.0%
ATTATAATTCCATCAAGGGT+TGG - chr8.2:54955206-54955225 None:intergenic 30.0%
GATGATGTTCCTAATTTCAA+AGG + chr8.2:54955371-54955390 MS.gene42755:CDS 30.0%
GCTCACAATAATTATTCTTG+GGG + chr8.2:54954128-54954147 MS.gene42755:intron 30.0%
GTGCTCACAATAATTATTCT+TGG + chr8.2:54954126-54954145 MS.gene42755:intron 30.0%
TAGGCATTATAATTCCATCA+AGG - chr8.2:54955211-54955230 None:intergenic 30.0%
TCAAAGAACTTAAAAGGGAA+AGG - chr8.2:54953557-54953576 None:intergenic 30.0%
TCACAAAAGTGAACTAAATG+AGG - chr8.2:54953509-54953528 None:intergenic 30.0%
TCTACAAATTAACACACAAG+AGG - chr8.2:54953368-54953387 None:intergenic 30.0%
TCTTTCCACTTCCATTAAAA+GGG - chr8.2:54955280-54955299 None:intergenic 30.0%
TGAGAATTCATCATAGTTGA+GGG - chr8.2:54952924-54952943 None:intergenic 30.0%
TTAGTTACGGTTACATACAA+TGG - chr8.2:54954232-54954251 None:intergenic 30.0%
TTGAAATTAGGAACATCATC+AGG - chr8.2:54955371-54955390 None:intergenic 30.0%
TTTGAACTGCAAAGAAATAC+TGG + chr8.2:54953785-54953804 MS.gene42755:CDS 30.0%
! AAAAGGACTAACCCTTTTAA+TGG + chr8.2:54955266-54955285 MS.gene42755:CDS 30.0%
! ACAAATTTCAAGGGTATGAA+TGG + chr8.2:54955056-54955075 MS.gene42755:CDS 30.0%
! ATAACCCTCATGATTGTTTT+TGG - chr8.2:54953398-54953417 None:intergenic 30.0%
! ATTAGTTTACTTGTGGTTGA+GGG - chr8.2:54953891-54953910 None:intergenic 30.0%
! TATTAGTTTACTTGTGGTTG+AGG - chr8.2:54953892-54953911 None:intergenic 30.0%
! TCAATAAGGTGGAATTGTTT+TGG + chr8.2:54954749-54954768 MS.gene42755:intron 30.0%
!! GTTGGAAGTGATTAATTTGT+AGG + chr8.2:54954911-54954930 MS.gene42755:intron 30.0%
!!! CACCAACAATGTTTTAGTTA+CGG - chr8.2:54954245-54954264 None:intergenic 30.0%
ACTAAAATCTCTCGATCTTC+CGG + chr8.2:54954847-54954866 MS.gene42755:intron 35.0%
ATTGCTCATTGAATTCCTTG+TGG - chr8.2:54953627-54953646 None:intergenic 35.0%
CACTCCAAAAACAATCATGA+GGG + chr8.2:54953391-54953410 MS.gene42755:CDS 35.0%
CTCTTTCCACTTCCATTAAA+AGG - chr8.2:54955281-54955300 None:intergenic 35.0%
GAAACGAATTCTTTCATGTC+AGG - chr8.2:54955095-54955114 None:intergenic 35.0%
GATCTTAGGCTTCATGATTT+GGG + chr8.2:54955323-54955342 MS.gene42755:CDS 35.0%
GTGGAAAGAGTCCAATAATT+TGG + chr8.2:54955291-54955310 MS.gene42755:CDS 35.0%
TCAATTAAGGATGGTATGAG+AGG + chr8.2:54953646-54953665 MS.gene42755:CDS 35.0%
TGATCTTAGGCTTCATGATT+TGG + chr8.2:54955322-54955341 MS.gene42755:CDS 35.0%
TGTCTTAATCCTATGATTGC+CGG - chr8.2:54954869-54954888 None:intergenic 35.0%
TTGCTCATTGAATTCCTTGT+GGG - chr8.2:54953626-54953645 None:intergenic 35.0%
TTTGTAGGTTTATATGTGGC+AGG + chr8.2:54954926-54954945 MS.gene42755:intron 35.0%
! ACTAACCCTTTTAATGGAAG+TGG + chr8.2:54955272-54955291 MS.gene42755:CDS 35.0%
! CATTGTTGGTGCAACATTTT+AGG + chr8.2:54954254-54954273 MS.gene42755:intron 35.0%
!! TGTATAGAGCTAGTCTTCTA+TGG + chr8.2:54954345-54954364 MS.gene42755:intron 35.0%
!! TTCAAAAGCACCTTCAATTG+AGG + chr8.2:54954949-54954968 MS.gene42755:CDS 35.0%
!!! ACATTTTAGGATGCAAATGG+AGG + chr8.2:54954267-54954286 MS.gene42755:intron 35.0%
!!! GCAACATTTTAGGATGCAAA+TGG + chr8.2:54954264-54954283 MS.gene42755:intron 35.0%
AAACCTGAAGATGGCTCTTA+AGG - chr8.2:54953448-54953467 None:intergenic 40.0%
AAAGCTTATCACACACTTGC+AGG + chr8.2:54955011-54955030 MS.gene42755:CDS 40.0%
AGCAGCAGCAACAAATTTCA+AGG + chr8.2:54955046-54955065 MS.gene42755:CDS 40.0%
AGGAGAAGATGAGTACTATC+AGG - chr8.2:54953071-54953090 None:intergenic 40.0%
ATCATAGTTGAGGGCTTCTT+TGG - chr8.2:54952915-54952934 None:intergenic 40.0%
ATGGCTCTTAAGGTGGTAAA+GGG - chr8.2:54953438-54953457 None:intergenic 40.0%
CCACTCCAAAAACAATCATG+AGG + chr8.2:54953390-54953409 MS.gene42755:CDS 40.0%
GCAGCAGCAACAAATTTCAA+GGG + chr8.2:54955047-54955066 MS.gene42755:CDS 40.0%
GTTATTCCAAGCTTCACAGA+TGG - chr8.2:54955476-54955495 None:intergenic 40.0%
TGCTCATTGAATTCCTTGTG+GGG - chr8.2:54953625-54953644 None:intergenic 40.0%
TGTGTGTTCAAACCTGAAGA+TGG - chr8.2:54953457-54953476 None:intergenic 40.0%
TTTGATCCATCTGTGAAGCT+TGG + chr8.2:54955467-54955486 MS.gene42755:CDS 40.0%
! AAGGGTATGAATGGAACTCA+AGG + chr8.2:54955065-54955084 MS.gene42755:CDS 40.0%
!!! CCTCATGATTGTTTTTGGAG+TGG - chr8.2:54953393-54953412 None:intergenic 40.0%
AAGACTGCATGAACAGCTAG+AGG + chr8.2:54954297-54954316 MS.gene42755:CDS 45.0%
AGAGTCACCTTGAACACACA+TGG - chr8.2:54952955-54952974 None:intergenic 45.0%
ATAGTTGAGGGCTTCTTTGG+AGG - chr8.2:54952912-54952931 None:intergenic 45.0%
ATGAGGGTTATGGGTGTGAA+GGG + chr8.2:54953407-54953426 MS.gene42755:CDS 45.0%
ATGATCAATGCTGTTGCAGC+TGG + chr8.2:54955503-54955522 MS.gene42755:CDS 45.0%
ATGTGTGTTCAAGGTGACTC+TGG + chr8.2:54952954-54952973 MS.gene42755:CDS 45.0%
CAATTGAGGATTGAGGCTCA+AGG + chr8.2:54954963-54954982 MS.gene42755:CDS 45.0%
CACACTTGCAGGTGAAAACA+TGG + chr8.2:54955022-54955041 MS.gene42755:CDS 45.0%
GAGCCTCAATCCTCAATTGA+AGG - chr8.2:54954962-54954981 None:intergenic 45.0%
GAGTCACCTTGAACACACAT+GGG - chr8.2:54952954-54952973 None:intergenic 45.0%
GATAGACCCATGTGTGTTCA+AGG + chr8.2:54952945-54952964 MS.gene42755:CDS 45.0%
GATGGCTCTTAAGGTGGTAA+AGG - chr8.2:54953439-54953458 None:intergenic 45.0%
GCACCTTCAATTGAGGATTG+AGG + chr8.2:54954956-54954975 MS.gene42755:CDS 45.0%
GGTAGAAGCTCATCACCATT+TGG + chr8.2:54955524-54955543 MS.gene42755:CDS 45.0%
TAGTTGAGGGCTTCTTTGGA+GGG - chr8.2:54952911-54952930 None:intergenic 45.0%
! AAAGCGTTCATGGAGTTCGA+CGG - chr8.2:54953021-54953040 None:intergenic 45.0%
! CAGCATCAACAAAGCGTTCA+TGG - chr8.2:54953031-54953050 None:intergenic 45.0%
!! GCTGCAACAGCATTGATCAT+AGG - chr8.2:54955503-54955522 None:intergenic 45.0%
!! TTAGAAAATAAAAATATTTA+AGG - chr8.2:54953694-54953713 None:intergenic 5.0%
ATAGAGAGGCCAACCCTTGA+TGG + chr8.2:54955194-54955213 MS.gene42755:CDS 50.0%
CATGAGGGTTATGGGTGTGA+AGG + chr8.2:54953406-54953425 MS.gene42755:CDS 50.0%
CCACCTTAAGAGCCATCTTC+AGG + chr8.2:54953442-54953461 MS.gene42755:CDS 50.0%
CCTGAAGATGGCTCTTAAGG+TGG - chr8.2:54953445-54953464 None:intergenic 50.0%
CTCGATCTTCCGGCAATCAT+AGG + chr8.2:54954857-54954876 MS.gene42755:intron 50.0%
GTTGATGCTGTTGCTCAGCT+TGG + chr8.2:54953041-54953060 MS.gene42755:CDS 50.0%
AAGGCGTGGTTTCGGATCAG+TGG - chr8.2:54952991-54953010 None:intergenic 55.0%
ATCCGAAACCACGCCTTCGA+TGG + chr8.2:54952994-54953013 MS.gene42755:CDS 55.0%
GATGCTGTTGCTCAGCTTGG+AGG + chr8.2:54953044-54953063 MS.gene42755:CDS 55.0%
GCGACCAGTCATAGAAGACG+AGG - chr8.2:54953813-54953832 None:intergenic 55.0%
GCTTGGAAAGCAACCCCACA+AGG + chr8.2:54953609-54953628 MS.gene42755:CDS 55.0%
GGCACCTCGTCTTCTATGAC+TGG + chr8.2:54953806-54953825 MS.gene42755:CDS 55.0%
GTGGTTTCGGATCAGTGGTG+AGG - chr8.2:54952986-54953005 None:intergenic 55.0%
TCGGATCAGTGGTGAGGACA+AGG - chr8.2:54952980-54952999 None:intergenic 55.0%
! GTCCATCGAAGGCGTGGTTT+CGG - chr8.2:54952999-54953018 None:intergenic 55.0%
GGAGTTCGACGGTCCATCGA+AGG - chr8.2:54953010-54953029 None:intergenic 60.0%
TCGACGGTCCATCGAAGGCG+TGG - chr8.2:54953005-54953024 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 54952894 54955550 54952894 ID=MS.gene42755
chr8.2 mRNA 54952894 54955550 54952894 ID=MS.gene42755.t1;Parent=MS.gene42755
chr8.2 exon 54952894 54953074 54952894 ID=MS.gene42755.t1.exon1;Parent=MS.gene42755.t1
chr8.2 CDS 54952894 54953074 54952894 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
chr8.2 exon 54953387 54953463 54953387 ID=MS.gene42755.t1.exon2;Parent=MS.gene42755.t1
chr8.2 CDS 54953387 54953463 54953387 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
chr8.2 exon 54953601 54953667 54953601 ID=MS.gene42755.t1.exon3;Parent=MS.gene42755.t1
chr8.2 CDS 54953601 54953667 54953601 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
chr8.2 exon 54953777 54953843 54953777 ID=MS.gene42755.t1.exon4;Parent=MS.gene42755.t1
chr8.2 CDS 54953777 54953843 54953777 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
chr8.2 exon 54954276 54954318 54954276 ID=MS.gene42755.t1.exon5;Parent=MS.gene42755.t1
chr8.2 CDS 54954276 54954318 54954276 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
chr8.2 exon 54954948 54955550 54954948 ID=MS.gene42755.t1.exon6;Parent=MS.gene42755.t1
chr8.2 CDS 54954948 54955550 54954948 ID=cds.MS.gene42755.t1;Parent=MS.gene42755.t1
Gene Sequence

>MS.gene42755

ATGGAAAGAATGTTCCCTCCAAAGAAGCCCTCAACTATGATGAATTCTCATGATAGACCCATGTGTGTTCAAGGTGACTCTGGCCTTGTCCTCACCACTGATCCGAAACCACGCCTTCGATGGACCGTCGAACTCCATGAACGCTTTGTTGATGCTGTTGCTCAGCTTGGAGGACCTGATAGTACTCATCTTCTCCTTTTATTCTTTTCTTTCTTTTGGTGCAAACTACATAACTAGAAAGAAAAAAAATACAAGTTTTTTTTTTGAAGAATATAACTAGAAGAAAAATGAAATTATGAAACTCTTCTTTAATTTTATCACTAAATTTTGTGATGAGTAGCCCTCAAAAAACAAAAACAAAAAATTGTGATGAGTGAATATAAATATAATTTATTTATTAGTCTTTAATTATATATTTTTTCTAGTAATATAATTTGCAACTTTTTTTCTAGTAATTTAATTTTTTTTTTTCCTCTTGTGTGTTAATTTGTAGAAGCCACTCCAAAAACAATCATGAGGGTTATGGGTGTGAAGGGTCTAACCCTTTACCACCTTAAGAGCCATCTTCAGGTTTGAACACACAAACTATATATTATTTTCCAAATTTCTTTACCTCATTTAGTTCACTTTTGTGACACTATCAATTTTTTTTTCTTTATACCTTTCCCTTTTAAGTTCTTTGATTTTATTTTCTTTTGCATTAATAGAAATTTAGGCTTGGAAAGCAACCCCACAAGGAATTCAATGAGCAATCAATTAAGGATGGTATGAGAGGTGACATATAACACTTTAATGTTCCTTAAATATTTTTATTTTCTAAGCTTTTATCTCTTTACACTTTGTTGAATTCACATGTATTATAACACTTTTGTTTTCATGACAGTTTCGGCTTTTGAACTGCAAAGAAATACTGGCACCTCGTCTTCTATGACTGGTCGCAACATGAATGAGTATGTTAATAATTACCTTTTGATTCTTAATTTATTTATTCAAACCCTCAACCACAAGTAAACTAATATTGAAAAGTATATAGTTTCTTCTCTCTTCTCTATACTTTCAGTCTTTTATTACTTTTTATTCTTTTTGTTTAGAGAAATTTTTCTTTAATTTGAATTTTTCTAGATGAATTGTTACATGAATCTATACTTAGTGCCTGCATATAATAACTAATAACTTCAACATGTATTTTCTTGCATTTTTATTAATTAATTTCAATTCATTTTTTTTTAATTGTGCTCACAATAATTATTCTTGGGGATTTGGATACATTAATTTAACTCATATTATAATGAGAAACTTTGGTCGATGTTTTAGTTGTACTGTGTTTTGTATGAGCCATTGTATGTAACCGTAACTAAAACATTGTTGGTGCAACATTTTAGGATGCAAATGGAGGTGCACAGAAGACTGCATGAACAGCTAGAGGTATACACAGCTACAAGTATCTCTAGTGTATAGAGCTAGTCTTCTATGGTCATAAGACGATATGTATAGTGTTTAGGCACACATACCGACTTTTTTTTTCCTAATAATAAAAAAATTAAATTAATTTAATATTTTGGAGACAAGTGCATCATTGTGTGTGTCTAAAAACTATACATTTGGTCTTGTGACCAAATAAGTATTTATCGTAACTATATTATTGAGTTTTGGTACTGCTAAGATGCAAAAAAGTTGAATTACCTAAAAATGTCTAAAAAAATTGAGAAAGTGAAAATTAAAACAAAGCTAGTTTCTAATTTCTAGGAGTTAACAACACTAAAATTAAATAACAATAATATTTTTCAATTAAATTAACTTTTTCAAGTATTATATGTCTGTCAAATAACAAAGCGATTGAGTTGGTTAATAAACTTCAATAAGGTGGAATTGTTTTGGAGAAATCCGAGTTCAAATCTTTCTTAAAACAGTGTTTTAATAGACTTTATTTATCTTCTGATTGAACTATAGATTACTAAAATCTCTCGATCTTCCGGCAATCATAGGATTAAGACAAAAAAATATATATGGTGCAAATGTTGGAAGTGATTAATTTGTAGGTTTATATGTGGCAGGTTCAAAAGCACCTTCAATTGAGGATTGAGGCTCAAGGAAAATACATGCAAAGTATATTGGAGAAAGCTTATCACACACTTGCAGGTGAAAACATGGCAGCAGCAGCAACAAATTTCAAGGGTATGAATGGAACTCAAGGCATCCCTGACATGAAAGAATTCGTTTCTCCTCTTAATAATTTTCCTCATTTTCAAGATCTTAACATTTGTGGTAGTGATGATGAACAACTTGATCTTCATCAAAACATAGAGAGGCCAACCCTTGATGGAATTATAATGCCTAATAATAATAATGATGAAAATTTGTTTGTTGGAAAGAAAAGGACTAACCCTTTTAATGGAAGTGGAAAGAGTCCAATAATTTGGAATGATAATGATCTTAGGCTTCATGATTTGGGAACAACTTCATCATGCATTAGTCCTGATGATGTTCCTAATTTCAAAGGTGAATCATTGGATAGATGTGATGAAAGTGATTCTATTGTTGATATTTATGACACAAAAGTGAGTGAGAAGAAATTTGATCCATCTGTGAAGCTTGGAATAACATTACCTATGATCAATGCTGTTGCAGCTGGTAGAAGCTCATCACCATTTGGTTGA
Protein sequence

>MS.gene42755.t1

MERMFPPKKPSTMMNSHDRPMCVQGDSGLVLTTDPKPRLRWTVELHERFVDAVAQLGGPDKATPKTIMRVMGVKGLTLYHLKSHLQKFRLGKQPHKEFNEQSIKDGMRVSAFELQRNTGTSSSMTGRNMNEMQMEVHRRLHEQLEVQKHLQLRIEAQGKYMQSILEKAYHTLAGENMAAAATNFKGMNGTQGIPDMKEFVSPLNNFPHFQDLNICGSDDEQLDLHQNIERPTLDGIIMPNNNNDENLFVGKKRTNPFNGSGKSPIIWNDNDLRLHDLGTTSSCISPDDVPNFKGESLDRCDESDSIVDIYDTKVSEKKFDPSVKLGITLPMINAVAAGRSSSPFG