AlfalfaGEDB | MS.gene42969

M. sativa cultivar XinJiangDaYe / MS.gene42969

KEGG: K14514
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0110165 GO:0140110
PFAM: PF04873.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42969.t1	RHN52064.1	86	228	22	1	1	218	4	231	4.50E-111	410.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42969.t1	Q9SLH0	53.7	227	93	2	1	215	107	333	1.3e-66	254.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42969.t1	G7KNC0	86.0	228	22	1	1	218	94	321	3.3e-111	410.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene42969.t1	TF	EIL

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42969.t1	MTR_6g060600	85.965	228	22	1	1	218	94	321	1.92e-143	415
MS.gene42969.t1	MTR_6g460640	85.526	228	23	1	1	218	94	321	8.04e-143	414
MS.gene42969.t1	MTR_3g010790	58.667	225	81	3	1	215	4	226	9.82e-88	268
MS.gene42969.t1	MTR_3g078770	54.783	230	86	4	1	218	109	332	4.75e-83	259
MS.gene42969.t1	MTR_5g087790	54.667	225	91	2	1	215	106	329	1.34e-81	255
MS.gene42969.t1	MTR_2g098470	55.838	197	74	3	1	187	97	290	3.23e-70	225
MS.gene42969.t1	MTR_3g087530	49.751	201	78	5	1	184	86	280	1.90e-64	206
MS.gene42969.t1	MTR_8g091370	49.239	197	86	4	1	184	76	271	2.73e-61	198
MS.gene42969.t1	MTR_1g024420	70.000	40	2	1	1	30	69	108	1.22e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42969.t1	AT2G27050	58.706	201	73	1	1	191	107	307	1.38e-80	251
MS.gene42969.t1	AT3G20770	58.621	203	69	3	1	191	106	305	3.29e-78	246
MS.gene42969.t1	AT5G10120	54.592	196	77	2	1	184	80	275	5.18e-70	221
MS.gene42969.t1	AT1G73730	54.822	197	74	3	1	187	97	288	1.92e-69	222
MS.gene42969.t1	AT1G73730	54.822	197	74	3	1	187	85	276	1.95e-69	222
MS.gene42969.t1	AT5G65100	51.546	194	83	2	1	183	110	303	1.21e-63	207
MS.gene42969.t1	AT5G21120	51.648	182	86	2	9	188	133	314	6.45e-59	194
MS.gene42969.t1	AT5G21120	51.648	182	86	2	9	188	126	307	7.03e-59	193
MS.gene42969.t1	AT5G21120	51.648	182	86	2	9	188	126	307	7.03e-59	193

Find 54 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAGATAAACTCAGTATTA+AGG	0.259065	6.3:-62771102	MS.gene42969:CDS
TTCAGCAAGTGAAGTGTAAT+TGG	0.259507	6.3:+62771416	None:intergenic
CGGAGATTTCCATTGGAAAA+AGG	0.266811	6.3:-62771326	MS.gene42969:CDS
GTATTAAGGAAACATCTATT+TGG	0.288262	6.3:-62771088	MS.gene42969:CDS
AGATTATGAGGCTTTCTATA+AGG	0.304992	6.3:+62771224	None:intergenic
GCTAAATACGAAACAGAAAA+TGG	0.309146	6.3:-62771476	MS.gene42969:CDS
GAACTACCCGACACCATATT+AGG	0.324492	6.3:-62771392	MS.gene42969:CDS
CCTCCACCATGGTGGCCAAC+TGG	0.352128	6.3:-62771299	MS.gene42969:CDS
GGTTTGGAAGGTTTATGTTT+TGG	0.353709	6.3:-62771195	MS.gene42969:CDS
ATAGTTAAATTTGACATAAA+CGG	0.356609	6.3:-62771512	MS.gene42969:CDS
TTCCTAATCTTATGAACATT+AGG	0.362413	6.3:+62771149	None:intergenic
GATTATGAGGCTTTCTATAA+GGG	0.363151	6.3:+62771225	None:intergenic
CAAACCTTCTTCAGATTATG+AGG	0.369249	6.3:+62771212	None:intergenic
TAGTTAAATTTGACATAAAC+GGG	0.385553	6.3:-62771511	MS.gene42969:CDS
CTCATAATCTGAAGAAGGTT+TGG	0.391254	6.3:-62771211	MS.gene42969:CDS
CAAATTATGACAAGTTGAAT+GGG	0.402527	6.3:-62771442	MS.gene42969:CDS
AGTTATGATTCTCGTGTTGA+AGG	0.407240	6.3:-62771005	MS.gene42969:CDS
ACAAATTATGACAAGTTGAA+TGG	0.414722	6.3:-62771443	MS.gene42969:CDS
CGCCATGCTTCTTTCCCAGT+TGG	0.424734	6.3:+62771284	None:intergenic
TGAGAGAAGTGAACCTAATA+TGG	0.432462	6.3:+62771379	None:intergenic
TTGTCTGATGAACCACTCAT+TGG	0.468990	6.3:+62771557	None:intergenic
CTCCTAATGTTCATAAGATT+AGG	0.475251	6.3:-62771151	MS.gene42969:CDS
TCTTTCCCAGTTGGCCACCA+TGG	0.483254	6.3:+62771293	None:intergenic
CATCAGACAATTTACGAGGC+TGG	0.493913	6.3:-62771544	MS.gene42969:CDS
AGAAGCATGGCGGAATGAGA+TGG	0.495357	6.3:-62771273	MS.gene42969:CDS
CTCCACCATGGTGGCCAACT+GGG	0.496691	6.3:-62771298	MS.gene42969:CDS
CCAGTTGGCCACCATGGTGG+AGG	0.511934	6.3:+62771299	None:intergenic
AATGGAAATCTCCGTTGTGG+TGG	0.514931	6.3:+62771335	None:intergenic
AGTGAACCTAATATGGTGTC+GGG	0.518068	6.3:+62771386	None:intergenic
ACCACAACGGAGATTTCCAT+TGG	0.522988	6.3:-62771333	MS.gene42969:CDS
TAATCTGAAGAAGGTTTGGA+AGG	0.542382	6.3:-62771207	MS.gene42969:CDS
GAAGCATGGCGGAATGAGAT+GGG	0.544110	6.3:-62771272	MS.gene42969:CDS
AAGTGAACCTAATATGGTGT+CGG	0.558954	6.3:+62771385	None:intergenic
AAAGCCTCATAATCTGAAGA+AGG	0.560995	6.3:-62771216	MS.gene42969:CDS
GGAACATAGTCAGACAATCA+AGG	0.561355	6.3:-62771130	MS.gene42969:CDS
TCGTATTTAGCAATTGCCGC+TGG	0.562404	6.3:+62771488	None:intergenic
TTCCCAGTTGGCCACCATGG+TGG	0.564701	6.3:+62771296	None:intergenic
TCCAATGGAAATCTCCGTTG+TGG	0.567968	6.3:+62771332	None:intergenic
GGCCAACTGGGAAAGAAGCA+TGG	0.577061	6.3:-62771286	MS.gene42969:CDS
ATGGGATTTAGTGAAGATCC+TGG	0.582998	6.3:-62771254	MS.gene42969:CDS
ATTATGAGGCTTTCTATAAG+GGG	0.590034	6.3:+62771226	None:intergenic
AAGTAATTATGTGCTTGTTG+AGG	0.609719	6.3:-62770982	MS.gene42969:CDS
ATGGAAATCTCCGTTGTGGT+GGG	0.614439	6.3:+62771336	None:intergenic
CAGACAATTTACGAGGCTGG+TGG	0.628174	6.3:-62771541	MS.gene42969:CDS
ATTTGACATAAACGGGCCAG+CGG	0.631675	6.3:-62771504	MS.gene42969:CDS
CGTTGTGGTGGGTCACAATG+CGG	0.634330	6.3:+62771347	None:intergenic
CGCATTGTGACCCACCACAA+CGG	0.638363	6.3:-62771346	MS.gene42969:CDS
TTATGAGGCTTTCTATAAGG+GGG	0.640626	6.3:+62771227	None:intergenic
AAATTATGACAAGTTGAATG+GGG	0.682321	6.3:-62771441	MS.gene42969:CDS
AAGGCATTCCTCCACCATGG+TGG	0.706316	6.3:-62771307	MS.gene42969:CDS
ATGGAAGTTTGTGATGCTCG+TGG	0.707841	6.3:-62771584	MS.gene42969:CDS
GCTCGTGGCAAACCAATGAG+TGG	0.727185	6.3:-62771569	MS.gene42969:CDS
GGTTCATCAGACAATTTACG+AGG	0.727611	6.3:-62771548	MS.gene42969:CDS
CAACTGGGAAAGAAGCATGG+CGG	0.774037	6.3:-62771283	MS.gene42969:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAGTTAAATTTGACATAAA+CGG	-	chr6.3:62771030-62771049	MS.gene42969:CDS	15.0%
!!	TAGTTAAATTTGACATAAAC+GGG	-	chr6.3:62771031-62771050	MS.gene42969:CDS	20.0%
!	ACAAGATAAACTCAGTATTA+AGG	-	chr6.3:62771440-62771459	MS.gene42969:CDS	25.0%
!	GTATTAAGGAAACATCTATT+TGG	-	chr6.3:62771454-62771473	MS.gene42969:CDS	25.0%
!	TCATAACTTGAGAAAAATTC+AGG	+	chr6.3:62771525-62771544	None:intergenic	25.0%
!	TTCCTAATCTTATGAACATT+AGG	+	chr6.3:62771396-62771415	None:intergenic	25.0%
!!	AAATTATGACAAGTTGAATG+GGG	-	chr6.3:62771101-62771120	MS.gene42969:CDS	25.0%
!!	ACAAATTATGACAAGTTGAA+TGG	-	chr6.3:62771099-62771118	MS.gene42969:CDS	25.0%
!!	CAAATTATGACAAGTTGAAT+GGG	-	chr6.3:62771100-62771119	MS.gene42969:CDS	25.0%
	AGATTATGAGGCTTTCTATA+AGG	+	chr6.3:62771321-62771340	None:intergenic	30.0%
	ATTATGAGGCTTTCTATAAG+GGG	+	chr6.3:62771319-62771338	None:intergenic	30.0%
	CTCCTAATGTTCATAAGATT+AGG	-	chr6.3:62771391-62771410	MS.gene42969:CDS	30.0%
	GATTATGAGGCTTTCTATAA+GGG	+	chr6.3:62771320-62771339	None:intergenic	30.0%
	GCTAAATACGAAACAGAAAA+TGG	-	chr6.3:62771066-62771085	MS.gene42969:CDS	30.0%
!	AAGTAATTATGTGCTTGTTG+AGG	-	chr6.3:62771560-62771579	MS.gene42969:CDS	30.0%
!!	AATGATTTTGATGTTGTGGA+AGG	-	chr6.3:62771585-62771604	MS.gene42969:CDS	30.0%
	AAAGCCTCATAATCTGAAGA+AGG	-	chr6.3:62771326-62771345	MS.gene42969:CDS	35.0%
	AAGTGAACCTAATATGGTGT+CGG	+	chr6.3:62771160-62771179	None:intergenic	35.0%
	AGTTATGATTCTCGTGTTGA+AGG	-	chr6.3:62771537-62771556	MS.gene42969:CDS	35.0%
	CAAACCTTCTTCAGATTATG+AGG	+	chr6.3:62771333-62771352	None:intergenic	35.0%
	CTCATAATCTGAAGAAGGTT+TGG	-	chr6.3:62771331-62771350	MS.gene42969:CDS	35.0%
	TAATCTGAAGAAGGTTTGGA+AGG	-	chr6.3:62771335-62771354	MS.gene42969:CDS	35.0%
	TGAGAGAAGTGAACCTAATA+TGG	+	chr6.3:62771166-62771185	None:intergenic	35.0%
	TTATGAGGCTTTCTATAAGG+GGG	+	chr6.3:62771318-62771337	None:intergenic	35.0%
	TTCAGCAAGTGAAGTGTAAT+TGG	+	chr6.3:62771129-62771148	None:intergenic	35.0%
!	GGTTTGGAAGGTTTATGTTT+TGG	-	chr6.3:62771347-62771366	MS.gene42969:CDS	35.0%
!!	GGCAAATGATTTTGATGTTG+TGG	-	chr6.3:62771581-62771600	MS.gene42969:CDS	35.0%
	AGTGAACCTAATATGGTGTC+GGG	+	chr6.3:62771159-62771178	None:intergenic	40.0%
	ATGGGATTTAGTGAAGATCC+TGG	-	chr6.3:62771288-62771307	MS.gene42969:CDS	40.0%
	CGGAGATTTCCATTGGAAAA+AGG	-	chr6.3:62771216-62771235	MS.gene42969:CDS	40.0%
	GGAACATAGTCAGACAATCA+AGG	-	chr6.3:62771412-62771431	MS.gene42969:CDS	40.0%
	GGTTCATCAGACAATTTACG+AGG	-	chr6.3:62770994-62771013	MS.gene42969:CDS	40.0%
	TTGTCTGATGAACCACTCAT+TGG	+	chr6.3:62770988-62771007	None:intergenic	40.0%
!!!	GAAGGTTTATGTTTTGGCAG+CGG	-	chr6.3:62771353-62771372	MS.gene42969:CDS	40.0%
	AATGGAAATCTCCGTTGTGG+TGG	+	chr6.3:62771210-62771229	None:intergenic	45.0%
	ACCACAACGGAGATTTCCAT+TGG	-	chr6.3:62771209-62771228	MS.gene42969:CDS	45.0%
	ATGGAAATCTCCGTTGTGGT+GGG	+	chr6.3:62771209-62771228	None:intergenic	45.0%
	ATGGAAGTTTGTGATGCTCG+TGG	-	chr6.3:62770958-62770977	MS.gene42969:CDS	45.0%
	ATTTGACATAAACGGGCCAG+CGG	-	chr6.3:62771038-62771057	MS.gene42969:CDS	45.0%
	CATCAGACAATTTACGAGGC+TGG	-	chr6.3:62770998-62771017	MS.gene42969:CDS	45.0%
	GAACTACCCGACACCATATT+AGG	-	chr6.3:62771150-62771169	MS.gene42969:CDS	45.0%
	TCCAATGGAAATCTCCGTTG+TGG	+	chr6.3:62771213-62771232	None:intergenic	45.0%
	TCGTATTTAGCAATTGCCGC+TGG	+	chr6.3:62771057-62771076	None:intergenic	45.0%
!	GGAGGAATGCCTTTTTCCAA+TGG	+	chr6.3:62771228-62771247	None:intergenic	45.0%
!!	AAAAAGGCATTCCTCCACCA+TGG	-	chr6.3:62771232-62771251	MS.gene42969:CDS	45.0%
	CAGACAATTTACGAGGCTGG+TGG	-	chr6.3:62771001-62771020	MS.gene42969:CDS	50.0%
	GAGGCTTTCTATAAGGGGGT+AGG	+	chr6.3:62771314-62771333	None:intergenic	50.0%
	TTTCTATAAGGGGGTAGGCC+AGG	+	chr6.3:62771309-62771328	None:intergenic	50.0%
!	AGAAGCATGGCGGAATGAGA+TGG	-	chr6.3:62771269-62771288	MS.gene42969:CDS	50.0%
!	CAACTGGGAAAGAAGCATGG+CGG	-	chr6.3:62771259-62771278	MS.gene42969:CDS	50.0%
!	GAAGCATGGCGGAATGAGAT+GGG	-	chr6.3:62771270-62771289	MS.gene42969:CDS	50.0%
	CGCATTGTGACCCACCACAA+CGG	-	chr6.3:62771196-62771215	MS.gene42969:CDS	55.0%
	CGCCATGCTTCTTTCCCAGT+TGG	+	chr6.3:62771261-62771280	None:intergenic	55.0%
	GCTCGTGGCAAACCAATGAG+TGG	-	chr6.3:62770973-62770992	MS.gene42969:CDS	55.0%
	GGCCAACTGGGAAAGAAGCA+TGG	-	chr6.3:62771256-62771275	MS.gene42969:CDS	55.0%
	TCTTTCCCAGTTGGCCACCA+TGG	+	chr6.3:62771252-62771271	None:intergenic	55.0%
!	AAGGCATTCCTCCACCATGG+TGG	-	chr6.3:62771235-62771254	MS.gene42969:CDS	55.0%
!!	CGTTGTGGTGGGTCACAATG+CGG	+	chr6.3:62771198-62771217	None:intergenic	55.0%
	CTCCACCATGGTGGCCAACT+GGG	-	chr6.3:62771244-62771263	MS.gene42969:CDS	60.0%
	TTCCCAGTTGGCCACCATGG+TGG	+	chr6.3:62771249-62771268	None:intergenic	60.0%
	CCAGTTGGCCACCATGGTGG+AGG	+	chr6.3:62771246-62771265	None:intergenic	65.0%
	CCTCCACCATGGTGGCCAAC+TGG	-	chr6.3:62771243-62771262	MS.gene42969:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	62770955	62771609	62770955	ID=MS.gene42969
chr6.3	mRNA	62770955	62771609	62770955	ID=MS.gene42969.t1;Parent=MS.gene42969
chr6.3	exon	62770955	62771609	62770955	ID=MS.gene42969.t1.exon1;Parent=MS.gene42969.t1
chr6.3	CDS	62770955	62771609	62770955	ID=cds.MS.gene42969.t1;Parent=MS.gene42969.t1

Gene Sequence

>MS.gene42969

ATGATGGAAGTTTGTGATGCTCGTGGCAAACCAATGAGTGGTTCATCAGACAATTTACGAGGCTGGTGGAAAGATATAGTTAAATTTGACATAAACGGGCCAGCGGCAATTGCTAAATACGAAACAGAAAATGGAGTTACAACAACAAATTATGACAAGTTGAATGGGGAACCAATTACACTTCACTTGCTGAATGAACTACCCGACACCATATTAGGTTCACTTCTCTCATCATTAGTGCCGCATTGTGACCCACCACAACGGAGATTTCCATTGGAAAAAGGCATTCCTCCACCATGGTGGCCAACTGGGAAAGAAGCATGGCGGAATGAGATGGGATTTAGTGAAGATCCTGGCCTACCCCCTTATAGAAAGCCTCATAATCTGAAGAAGGTTTGGAAGGTTTATGTTTTGGCAGCGGTTATTAAACATATGTCTCCTAATGTTCATAAGATTAGGAACATAGTCAGACAATCAAGGAGTTTACAAGATAAACTCAGTATTAAGGAAACATCTATTTGGTTTGCTATTATTAATCATGAAGAAACAATTGCAAGAAAAATACATCCTGAATTTTTCTCAAGTTATGATTCTCGTGTTGAAGGAAGTAATTATGTGCTTGTTGAGGCAAATGATTTTGATGTTGTGGAAGGTG

Protein sequence

>MS.gene42969.t1

MMEVCDARGKPMSGSSDNLRGWWKDIVKFDINGPAAIAKYETENGVTTTNYDKLNGEPITLHLLNELPDTILGSLLSSLVPHCDPPQRRFPLEKGIPPPWWPTGKEAWRNEMGFSEDPGLPPYRKPHNLKKVWKVYVLAAVIKHMSPNVHKIRNIVRQSRSLQDKLSIKETSIWFAIINHEETIARKIHPEFFSSYDSRVEGSNYVLVEANDFDVVEG