Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43263.t1 | XP_013457839.1 | 80.9 | 293 | 18 | 1 | 1 | 293 | 1 | 255 | 1.10E-104 | 389.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43263.t1 | Q9FJK3 | 29.1 | 110 | 74 | 3 | 1 | 109 | 1 | 107 | 2.0e-06 | 54.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43263.t1 | A0A072V1C0 | 80.9 | 293 | 18 | 1 | 1 | 293 | 1 | 255 | 8.0e-105 | 389.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene43263.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43263.t1 | MTR_4g107170 | 78.498 | 293 | 25 | 2 | 1 | 293 | 1 | 255 | 1.30e-165 | 460 |
| MS.gene43263.t1 | MTR_4g045977 | 47.801 | 341 | 114 | 6 | 1 | 293 | 1 | 325 | 8.56e-95 | 285 |
| MS.gene43263.t1 | MTR_2g049610 | 57.692 | 156 | 63 | 1 | 1 | 156 | 1 | 153 | 1.01e-58 | 188 |
| MS.gene43263.t1 | MTR_2g049630 | 46.939 | 196 | 97 | 5 | 1 | 191 | 1 | 194 | 4.50e-51 | 172 |
| MS.gene43263.t1 | MTR_2g049530 | 49.383 | 162 | 79 | 3 | 3 | 162 | 5 | 165 | 1.55e-46 | 155 |
| MS.gene43263.t1 | MTR_1g115290 | 61.538 | 117 | 43 | 1 | 38 | 152 | 2 | 118 | 2.55e-44 | 148 |
| MS.gene43263.t1 | MTR_6g018920 | 38.065 | 155 | 93 | 1 | 1 | 152 | 1 | 155 | 7.73e-35 | 129 |
| MS.gene43263.t1 | MTR_1g114730 | 41.290 | 155 | 88 | 1 | 1 | 152 | 1 | 155 | 2.12e-34 | 128 |
| MS.gene43263.t1 | MTR_6g005440 | 39.103 | 156 | 92 | 1 | 1 | 153 | 1 | 156 | 1.18e-33 | 125 |
| MS.gene43263.t1 | MTR_6g005450 | 36.774 | 155 | 95 | 1 | 1 | 152 | 1 | 155 | 9.14e-29 | 112 |
| MS.gene43263.t1 | MTR_3g466980 | 30.882 | 136 | 88 | 4 | 1 | 135 | 1 | 131 | 1.65e-13 | 68.6 |
| MS.gene43263.t1 | MTR_2g016210 | 25.161 | 155 | 105 | 3 | 2 | 148 | 3 | 154 | 1.04e-11 | 63.9 |
| MS.gene43263.t1 | MTR_3g031240 | 30.841 | 107 | 72 | 1 | 1 | 107 | 1 | 105 | 3.11e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43263.t1 | AT5G55690 | 30.464 | 151 | 94 | 2 | 1 | 147 | 1 | 144 | 5.37e-16 | 76.6 |
| MS.gene43263.t1 | AT5G55690 | 30.464 | 151 | 94 | 2 | 1 | 147 | 1 | 144 | 5.37e-16 | 76.6 |
Find 53 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACACTTGTAAGGTCTTC+TGG | 0.270047 | 8.4:-20121103 | None:intergenic |
| TCCTGAGATATTTGATGTTA+AGG | 0.348371 | 8.4:+20121168 | MS.gene43263:CDS |
| GCACTTTGGATGTATTTCAT+AGG | 0.357011 | 8.4:-20120941 | None:intergenic |
| GTCAACCTGATCAGTTTGTT+TGG | 0.365476 | 8.4:+20121764 | MS.gene43263:CDS |
| AGCCCACTAAGTTGGTTTGA+TGG | 0.416723 | 8.4:-20121463 | None:intergenic |
| ACCATCTCACTTGTATAATC+AGG | 0.418843 | 8.4:-20121514 | None:intergenic |
| GATCAACAAGTTGACTAAGT+TGG | 0.422061 | 8.4:-20121693 | None:intergenic |
| CTCCACAAGCCCACTAAGTT+GGG | 0.432529 | 8.4:-20121581 | None:intergenic |
| ACAATCTCACTCATATAATC+AGG | 0.433010 | 8.4:-20121625 | None:intergenic |
| GCAAGGAGTTTACACTTGTA+AGG | 0.440354 | 8.4:-20121111 | None:intergenic |
| AGTTGGTTTGCTGATGAACT+TGG | 0.448642 | 8.4:-20121676 | None:intergenic |
| AGTTGGTTTGATGGTGAACT+AGG | 0.459766 | 8.4:-20121454 | None:intergenic |
| TCTCCACAAGCCCACTAAGT+TGG | 0.464358 | 8.4:-20121582 | None:intergenic |
| TCTTTCCAAACAAACTGATC+AGG | 0.470431 | 8.4:-20121769 | None:intergenic |
| CTAAAATGTGGATGCCAAGT+TGG | 0.479466 | 8.4:-20121268 | None:intergenic |
| TAAAATGTGGATGCCAAGTT+GGG | 0.491000 | 8.4:-20121267 | None:intergenic |
| GCTTGCTTTATTGTGTATGA+TGG | 0.491871 | 8.4:+20121049 | MS.gene43263:CDS |
| TCATCATCAACCCAACTTAG+TGG | 0.494369 | 8.4:+20121571 | MS.gene43263:CDS |
| TCCTTAACATCAAATATCTC+AGG | 0.501707 | 8.4:-20121169 | None:intergenic |
| TCTCCACAAGCCCACTAAGT+TGG | 0.502348 | 8.4:-20121471 | None:intergenic |
| CTTTGAAAATAAGACAGAAA+AGG | 0.506269 | 8.4:+20121195 | MS.gene43263:CDS |
| TTGTAAGAAGTCTGGAGCAA+AGG | 0.512867 | 8.4:+20121027 | MS.gene43263:CDS |
| AACTTGTTGATCTTGATGAC+TGG | 0.515181 | 8.4:+20121704 | MS.gene43263:CDS |
| TCACCATCAAACCAACTTAG+TGG | 0.519319 | 8.4:+20121460 | MS.gene43263:CDS |
| TATTGCGAGATTTCGCACTT+TGG | 0.522492 | 8.4:-20120955 | None:intergenic |
| ATTGTGGATTTCACTAGTCT+TGG | 0.527589 | 8.4:+20121643 | MS.gene43263:CDS |
| CATCATCAACCCAACTTAGT+GGG | 0.531094 | 8.4:+20121572 | MS.gene43263:CDS |
| AACCCAACTTAGTGGGCTTG+TGG | 0.535603 | 8.4:+20121579 | MS.gene43263:CDS |
| TATAATCAGGATTAACAACT+TGG | 0.538229 | 8.4:-20121612 | None:intergenic |
| AAACCAACTTAGTGGGCTTG+TGG | 0.552156 | 8.4:+20121468 | MS.gene43263:CDS |
| AGGTCTTCTGGCCATGTCGT+AGG | 0.557005 | 8.4:-20121091 | None:intergenic |
| TGATTATATGAGTGAGATTG+TGG | 0.565961 | 8.4:+20121627 | MS.gene43263:CDS |
| TCATCCAAGTTACTAAAATG+TGG | 0.567439 | 8.4:-20121280 | None:intergenic |
| ATGGTGGATTTCACAAGTCT+TGG | 0.570027 | 8.4:+20121532 | MS.gene43263:CDS |
| TGGTTTGCTGATGAACTTGG+AGG | 0.570787 | 8.4:-20121673 | None:intergenic |
| ACTTTCAAACAAAGAAAGCA+TGG | 0.571291 | 8.4:+20120980 | MS.gene43263:CDS |
| TCAGTAAGATCTACCCAACT+TGG | 0.575658 | 8.4:+20121254 | MS.gene43263:CDS |
| TATAATCAGGATCAACAACT+TGG | 0.576059 | 8.4:-20121501 | None:intergenic |
| CACCATCAAACCAACTTAGT+GGG | 0.576425 | 8.4:+20121461 | MS.gene43263:CDS |
| AGTGGGCTTGTGGAGAGGAA+TGG | 0.577682 | 8.4:+20121478 | MS.gene43263:CDS |
| ATTCTGATACTCTTGAAGCA+AGG | 0.580425 | 8.4:-20121128 | None:intergenic |
| AACTTAGTGGGCTTGTGGAG+AGG | 0.584533 | 8.4:+20121473 | MS.gene43263:CDS |
| TGATTATACAAGTGAGATGG+TGG | 0.591946 | 8.4:+20121516 | MS.gene43263:CDS |
| ACAAATAGAAACTAGCACCT+TGG | 0.593239 | 8.4:+20121387 | MS.gene43263:CDS |
| AGTTGGGTTGATGATGAACT+AGG | 0.593899 | 8.4:-20121565 | None:intergenic |
| TGGTTTGATGGTGAACTAGG+AGG | 0.598871 | 8.4:-20121451 | None:intergenic |
| AATCATCTCACATATATCCA+AGG | 0.609547 | 8.4:-20121404 | None:intergenic |
| TGGATAAATCAATTAGATGA+CGG | 0.620408 | 8.4:+20121724 | MS.gene43263:CDS |
| AAACAAAGATTAAGAAAGTG+CGG | 0.626571 | 8.4:+20121221 | MS.gene43263:CDS |
| TCCTGATTATACAAGTGAGA+TGG | 0.678910 | 8.4:+20121513 | MS.gene43263:CDS |
| TGGGTTGATGATGAACTAGG+AGG | 0.698401 | 8.4:-20121562 | None:intergenic |
| ATAATATTGAACCTACGACA+TGG | 0.717032 | 8.4:+20121080 | MS.gene43263:CDS |
| AAGTTGGGTAGATCTTACTG+AGG | 0.750378 | 8.4:-20121252 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACAAAGATTAAGAAAGTG+CGG | + | chr8.4:20121221-20121240 | MS.gene43263:CDS | 25.0% |
| ! | TATAATCAGGATTAACAACT+TGG | - | chr8.4:20121615-20121634 | None:intergenic | 25.0% |
| ! | TGGATAAATCAATTAGATGA+CGG | + | chr8.4:20121724-20121743 | MS.gene43263:CDS | 25.0% |
| !! | CTTTGAAAATAAGACAGAAA+AGG | + | chr8.4:20121195-20121214 | MS.gene43263:CDS | 25.0% |
| !!! | ATGATTGATTTTTCTGATCT+TGG | + | chr8.4:20121421-20121440 | MS.gene43263:CDS | 25.0% |
| AATCATCTCACATATATCCA+AGG | - | chr8.4:20121407-20121426 | None:intergenic | 30.0% | |
| ACAATCTCACTCATATAATC+AGG | - | chr8.4:20121628-20121647 | None:intergenic | 30.0% | |
| ACTTTCAAACAAAGAAAGCA+TGG | + | chr8.4:20120980-20120999 | MS.gene43263:CDS | 30.0% | |
| ATAATATTGAACCTACGACA+TGG | + | chr8.4:20121080-20121099 | MS.gene43263:CDS | 30.0% | |
| TATAATCAGGATCAACAACT+TGG | - | chr8.4:20121504-20121523 | None:intergenic | 30.0% | |
| TCATCCAAGTTACTAAAATG+TGG | - | chr8.4:20121283-20121302 | None:intergenic | 30.0% | |
| TCCTGAGATATTTGATGTTA+AGG | + | chr8.4:20121168-20121187 | MS.gene43263:CDS | 30.0% | |
| TCCTTAACATCAAATATCTC+AGG | - | chr8.4:20121172-20121191 | None:intergenic | 30.0% | |
| TGATTATATGAGTGAGATTG+TGG | + | chr8.4:20121627-20121646 | MS.gene43263:CDS | 30.0% | |
| ! | TCTGAATTTTGTAAGAAGTC+TGG | + | chr8.4:20121019-20121038 | MS.gene43263:CDS | 30.0% |
| ACAAATAGAAACTAGCACCT+TGG | + | chr8.4:20121387-20121406 | MS.gene43263:CDS | 35.0% | |
| ACCATCTCACTTGTATAATC+AGG | - | chr8.4:20121517-20121536 | None:intergenic | 35.0% | |
| ATTCTGATACTCTTGAAGCA+AGG | - | chr8.4:20121131-20121150 | None:intergenic | 35.0% | |
| GCACTTTGGATGTATTTCAT+AGG | - | chr8.4:20120944-20120963 | None:intergenic | 35.0% | |
| TAAAATGTGGATGCCAAGTT+GGG | - | chr8.4:20121270-20121289 | None:intergenic | 35.0% | |
| TCCTGATTATACAAGTGAGA+TGG | + | chr8.4:20121513-20121532 | MS.gene43263:CDS | 35.0% | |
| TCTTTCCAAACAAACTGATC+AGG | - | chr8.4:20121772-20121791 | None:intergenic | 35.0% | |
| TGATTATACAAGTGAGATGG+TGG | + | chr8.4:20121516-20121535 | MS.gene43263:CDS | 35.0% | |
| TTTACACTTGTAAGGTCTTC+TGG | - | chr8.4:20121106-20121125 | None:intergenic | 35.0% | |
| ! | ATTGTGGATTTCACTAGTCT+TGG | + | chr8.4:20121643-20121662 | MS.gene43263:CDS | 35.0% |
| ! | GATCAACAAGTTGACTAAGT+TGG | - | chr8.4:20121696-20121715 | None:intergenic | 35.0% |
| ! | GCTTGCTTTATTGTGTATGA+TGG | + | chr8.4:20121049-20121068 | MS.gene43263:CDS | 35.0% |
| !! | AACTTGTTGATCTTGATGAC+TGG | + | chr8.4:20121704-20121723 | MS.gene43263:CDS | 35.0% |
| !! | GCATCCACATTTTAGTAACT+TGG | + | chr8.4:20121276-20121295 | MS.gene43263:CDS | 35.0% |
| AAGTTGGGTAGATCTTACTG+AGG | - | chr8.4:20121255-20121274 | None:intergenic | 40.0% | |
| CACCATCAAACCAACTTAGT+GGG | + | chr8.4:20121461-20121480 | MS.gene43263:CDS | 40.0% | |
| CATCATCAACCCAACTTAGT+GGG | + | chr8.4:20121572-20121591 | MS.gene43263:CDS | 40.0% | |
| CTAAAATGTGGATGCCAAGT+TGG | - | chr8.4:20121271-20121290 | None:intergenic | 40.0% | |
| GCAAGGAGTTTACACTTGTA+AGG | - | chr8.4:20121114-20121133 | None:intergenic | 40.0% | |
| TATTGCGAGATTTCGCACTT+TGG | - | chr8.4:20120958-20120977 | None:intergenic | 40.0% | |
| TCACCATCAAACCAACTTAG+TGG | + | chr8.4:20121460-20121479 | MS.gene43263:CDS | 40.0% | |
| TCAGTAAGATCTACCCAACT+TGG | + | chr8.4:20121254-20121273 | MS.gene43263:CDS | 40.0% | |
| TCATCATCAACCCAACTTAG+TGG | + | chr8.4:20121571-20121590 | MS.gene43263:CDS | 40.0% | |
| ! | GTCAACCTGATCAGTTTGTT+TGG | + | chr8.4:20121764-20121783 | MS.gene43263:CDS | 40.0% |
| ! | TTGTAAGAAGTCTGGAGCAA+AGG | + | chr8.4:20121027-20121046 | MS.gene43263:CDS | 40.0% |
| !! | AGTTGGGTTGATGATGAACT+AGG | - | chr8.4:20121568-20121587 | None:intergenic | 40.0% |
| !! | AGTTGGTTTGATGGTGAACT+AGG | - | chr8.4:20121457-20121476 | None:intergenic | 40.0% |
| !! | AGTTGGTTTGCTGATGAACT+TGG | - | chr8.4:20121679-20121698 | None:intergenic | 40.0% |
| !! | ATGGTGGATTTCACAAGTCT+TGG | + | chr8.4:20121532-20121551 | MS.gene43263:CDS | 40.0% |
| !! | AAACCAACTTAGTGGGCTTG+TGG | + | chr8.4:20121468-20121487 | MS.gene43263:CDS | 45.0% |
| !! | AGATGACGGTGTTTTGTGCT+GGG | + | chr8.4:20121738-20121757 | MS.gene43263:CDS | 45.0% |
| !! | AGCCCACTAAGTTGGTTTGA+TGG | - | chr8.4:20121466-20121485 | None:intergenic | 45.0% |
| !! | TAGATGACGGTGTTTTGTGC+TGG | + | chr8.4:20121737-20121756 | MS.gene43263:CDS | 45.0% |
| !! | TGGGTTGATGATGAACTAGG+AGG | - | chr8.4:20121565-20121584 | None:intergenic | 45.0% |
| !! | TGGTTTGATGGTGAACTAGG+AGG | - | chr8.4:20121454-20121473 | None:intergenic | 45.0% |
| !! | TGGTTTGCTGATGAACTTGG+AGG | - | chr8.4:20121676-20121695 | None:intergenic | 45.0% |
| CTCCACAAGCCCACTAAGTT+GGG | - | chr8.4:20121473-20121492 | None:intergenic | 50.0% | |
| TCTCCACAAGCCCACTAAGT+TGG | - | chr8.4:20121474-20121493 | None:intergenic | 50.0% | |
| !! | AACCCAACTTAGTGGGCTTG+TGG | + | chr8.4:20121579-20121598 | MS.gene43263:CDS | 50.0% |
| !! | AACTTAGTGGGCTTGTGGAG+AGG | + | chr8.4:20121473-20121492 | MS.gene43263:CDS | 50.0% |
| AGGTCTTCTGGCCATGTCGT+AGG | - | chr8.4:20121094-20121113 | None:intergenic | 55.0% | |
| ! | AGTGGGCTTGTGGAGAGGAA+TGG | + | chr8.4:20121478-20121497 | MS.gene43263:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 20120923 | 20121804 | 20120923 | ID=MS.gene43263 |
| chr8.4 | mRNA | 20120923 | 20121804 | 20120923 | ID=MS.gene43263.t1;Parent=MS.gene43263 |
| chr8.4 | exon | 20120923 | 20121804 | 20120923 | ID=MS.gene43263.t1.exon1;Parent=MS.gene43263.t1 |
| chr8.4 | CDS | 20120923 | 20121804 | 20120923 | ID=cds.MS.gene43263.t1;Parent=MS.gene43263.t1 |
| Gene Sequence |
| Protein sequence |
