AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43540.t1 MTR_5g015490 97.691 433 8 1 1 431 59 491 0.0 880
MS.gene43540.t1 MTR_4g097080 69.799 447 108 10 2 431 68 504 0.0 615
MS.gene43540.t1 MTR_2g099110 54.798 396 154 7 50 431 68 452 3.57e-146 424
MS.gene43540.t1 MTR_2g089100 31.336 434 269 7 21 431 17 444 1.90e-62 209
MS.gene43540.t1 MTR_5g097480 30.000 390 252 7 48 430 169 544 8.02e-56 193
MS.gene43540.t1 MTR_2g082090 32.021 381 240 7 56 430 212 579 9.83e-56 194
MS.gene43540.t1 MTR_1g029420 31.234 381 245 8 55 430 224 592 1.05e-55 194
MS.gene43540.t1 MTR_5g094450 30.809 383 246 7 56 430 161 532 3.25e-54 189
MS.gene43540.t1 MTR_4g095500 28.665 457 279 10 17 431 18 469 2.77e-52 182
MS.gene43540.t1 MTR_3g089055 31.234 381 245 8 55 430 202 570 3.08e-52 184
MS.gene43540.t1 MTR_3g089055 31.234 381 245 8 55 430 197 565 3.45e-52 184
MS.gene43540.t1 MTR_3g056110 29.630 405 239 12 51 430 159 542 8.12e-51 180
MS.gene43540.t1 MTR_5g015950 28.442 443 257 12 36 431 18 447 7.04e-49 172
MS.gene43540.t1 MTR_3g053270 28.442 443 257 12 36 431 18 447 7.04e-49 172
MS.gene43540.t1 MTR_7g027190 31.234 397 230 12 55 430 299 673 2.09e-47 172
MS.gene43540.t1 MTR_3g065980 28.228 457 276 9 3 430 107 540 5.14e-46 167
MS.gene43540.t1 MTR_2g026250 29.444 360 233 8 78 430 251 596 1.29e-45 167
MS.gene43540.t1 MTR_4g133660 29.167 384 246 10 56 429 175 542 1.05e-44 163
MS.gene43540.t1 MTR_8g020840 31.436 404 234 13 50 430 165 548 4.89e-44 162
MS.gene43540.t1 MTR_8g442410 29.474 380 252 9 57 430 156 525 3.94e-42 156
MS.gene43540.t1 MTR_4g102790 27.979 386 256 11 55 431 291 663 5.32e-42 157
MS.gene43540.t1 MTR_7g074650 28.571 378 240 13 59 431 441 793 2.97e-41 156
MS.gene43540.t1 MTR_4g064200 29.058 382 251 9 56 431 284 651 1.24e-40 153
MS.gene43540.t1 MTR_4g077760 29.870 385 236 11 57 430 194 555 2.36e-38 146
MS.gene43540.t1 MTR_4g064150 26.842 380 270 4 55 431 360 734 3.10e-38 147
MS.gene43540.t1 MTR_5g009080 25.806 496 280 13 1 430 5 478 1.47e-37 142
MS.gene43540.t1 MTR_2g097310 26.579 380 263 9 56 431 272 639 6.18e-37 143
MS.gene43540.t1 MTR_2g097410 28.534 382 257 7 56 431 371 742 6.50e-37 143
MS.gene43540.t1 MTR_4g064120 28.205 351 240 7 55 400 253 596 2.51e-36 141
MS.gene43540.t1 MTR_4g064160 26.913 379 265 5 56 431 316 685 8.73e-35 137
MS.gene43540.t1 MTR_4g064180 28.205 351 240 7 55 400 253 596 9.20e-35 136
MS.gene43540.t1 MTR_7g062120 25.330 379 271 5 56 431 372 741 1.04e-34 137
MS.gene43540.t1 MTR_2g097350 25.526 380 267 8 56 431 274 641 1.79e-34 135
MS.gene43540.t1 MTR_4g104020 25.897 390 258 10 55 430 148 520 1.99e-34 134
MS.gene43540.t1 MTR_2g097467 25.654 382 263 7 56 431 289 655 3.07e-34 135
MS.gene43540.t1 MTR_2g097390 26.316 380 264 9 56 431 275 642 1.22e-33 133
MS.gene43540.t1 MTR_2g097463 26.178 382 261 7 56 431 290 656 3.54e-33 132
MS.gene43540.t1 MTR_2g097473 25.916 382 262 7 56 431 289 655 9.20e-33 131
MS.gene43540.t1 MTR_3g022830 23.765 425 259 9 57 430 26 436 3.56e-32 127
MS.gene43540.t1 MTR_4g076020 26.984 378 237 14 59 431 87 430 1.72e-31 125
MS.gene43540.t1 MTR_1g069725 27.034 381 244 14 56 429 109 462 1.38e-29 120
MS.gene43540.t1 MTR_3g072710 27.654 405 239 15 55 429 119 499 6.70e-28 115
MS.gene43540.t1 MTR_1g096030 27.094 406 241 14 55 430 79 459 1.34e-26 111
MS.gene43540.t1 MTR_4g076140 29.960 247 163 4 36 281 28 265 1.17e-25 108
MS.gene43540.t1 MTR_2g034260 24.479 384 270 8 57 430 211 584 1.01e-24 107
MS.gene43540.t1 MTR_8g093070 26.804 388 245 17 55 431 138 497 1.77e-24 105
MS.gene43540.t1 MTR_2g097380 26.852 324 210 9 59 380 227 525 2.17e-24 105
MS.gene43540.t1 MTR_5g058860 27.778 396 248 14 55 429 118 496 3.49e-24 105
MS.gene43540.t1 MTR_2g034280 25.258 388 260 12 57 430 204 575 5.13e-24 105
MS.gene43540.t1 MTR_7g057230 24.816 407 279 9 34 429 266 656 8.91e-23 101
MS.gene43540.t1 MTR_2g034250 23.896 385 272 8 57 430 211 585 1.76e-22 100
MS.gene43540.t1 MTR_6g047750 26.166 386 254 11 57 430 296 662 2.71e-21 97.1
MS.gene43540.t1 MTR_1g086970 25.208 361 249 10 78 430 131 478 2.98e-20 93.2
MS.gene43540.t1 MTR_7g109580 23.670 376 274 6 57 430 190 554 1.10e-18 88.6
MS.gene43540.t1 MTR_4g026485 21.277 376 273 8 57 430 270 624 6.07e-16 80.5
MS.gene43540.t1 MTR_0092s0100 21.374 393 253 9 57 430 373 728 1.60e-13 73.2
MS.gene43540.t1 MTR_0092s0100 21.374 393 253 9 57 430 376 731 1.83e-13 72.8
MS.gene43540.t1 MTR_8g077940 23.037 382 258 10 54 430 191 541 3.26e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43540.t1 AT4G37650 65.865 416 124 7 29 431 120 530 0.0 554
MS.gene43540.t1 AT2G04890 31.649 376 241 7 56 430 53 413 2.53e-60 202
MS.gene43540.t1 AT1G21450 32.902 386 233 9 55 430 224 593 1.61e-52 186
MS.gene43540.t1 AT3G49950 31.818 396 246 9 50 431 24 409 2.64e-52 181
MS.gene43540.t1 AT5G48150 28.647 377 257 6 57 430 123 490 4.57e-52 182
MS.gene43540.t1 AT5G48150 28.647 377 257 6 57 430 123 490 4.57e-52 182
MS.gene43540.t1 AT5G48150 28.647 377 257 6 57 430 123 490 4.57e-52 182
MS.gene43540.t1 AT5G48150 28.647 377 257 6 57 430 123 490 4.57e-52 182
MS.gene43540.t1 AT1G50600 30.469 384 242 9 56 430 159 526 8.00e-51 179
MS.gene43540.t1 AT1G50600 30.469 384 242 9 56 430 230 597 1.95e-50 180
MS.gene43540.t1 AT1G50600 30.469 384 242 9 56 430 234 601 1.96e-50 180
MS.gene43540.t1 AT3G03450 30.570 386 248 7 49 430 176 545 2.46e-49 176
MS.gene43540.t1 AT1G66350 30.858 431 268 12 3 430 103 506 1.18e-48 173
MS.gene43540.t1 AT5G17490 31.783 387 242 8 46 430 150 516 4.09e-48 172
MS.gene43540.t1 AT2G01570 29.443 377 243 10 57 430 225 581 9.51e-43 159
MS.gene43540.t1 AT4G17230 26.371 383 261 8 56 431 158 526 1.61e-41 154
MS.gene43540.t1 AT4G17230 26.371 383 261 8 56 431 158 526 1.61e-41 154
MS.gene43540.t1 AT1G14920 29.708 377 242 9 57 430 173 529 1.62e-41 154
MS.gene43540.t1 AT1G50420 25.532 470 280 7 21 430 20 479 2.30e-41 153
MS.gene43540.t1 AT3G13840 30.982 397 230 14 50 430 142 510 7.00e-40 149
MS.gene43540.t1 AT3G54220 29.058 382 236 15 59 431 296 651 1.06e-38 148
MS.gene43540.t1 AT1G63100 29.412 391 247 11 55 429 276 653 4.06e-38 146
MS.gene43540.t1 AT1G63100 29.412 391 247 11 55 429 251 628 4.22e-38 146
MS.gene43540.t1 AT5G41920 29.243 383 240 14 55 430 42 400 1.47e-35 135
MS.gene43540.t1 AT1G55580 28.398 412 248 11 56 430 44 445 8.67e-35 134
MS.gene43540.t1 AT3G46600 27.559 381 261 8 55 431 3 372 1.33e-34 132
MS.gene43540.t1 AT2G29065 26.494 385 267 9 56 431 252 629 3.24e-34 135
MS.gene43540.t1 AT5G66770 28.191 376 243 10 64 430 227 584 7.05e-34 134
MS.gene43540.t1 AT2G37650 26.316 380 266 6 55 431 346 714 9.47e-34 134
MS.gene43540.t1 AT2G37650 26.316 380 266 6 55 431 334 702 9.57e-34 134
MS.gene43540.t1 AT3G46600 27.559 381 261 8 55 431 211 580 1.30e-33 133
MS.gene43540.t1 AT3G46600 27.559 381 261 8 55 431 179 548 1.47e-33 132
MS.gene43540.t1 AT5G52510 27.296 392 247 9 57 430 269 640 2.36e-33 132
MS.gene43540.t1 AT1G07530 25.526 380 270 6 56 431 396 766 5.60e-33 132
MS.gene43540.t1 AT2G29060 25.131 382 271 8 56 431 321 693 4.36e-30 123
MS.gene43540.t1 AT2G29060 25.131 382 271 8 56 431 284 656 4.38e-30 123
MS.gene43540.t1 AT1G07520 25.131 382 275 7 56 431 154 530 5.51e-30 122
MS.gene43540.t1 AT1G07520 25.131 382 275 7 56 431 154 530 5.51e-30 122
MS.gene43540.t1 AT1G07520 25.131 382 275 7 56 431 318 694 5.84e-30 123
MS.gene43540.t1 AT1G07520 25.131 382 275 7 56 431 319 695 6.02e-30 123
MS.gene43540.t1 AT3G50650 28.934 394 238 12 54 430 174 542 6.09e-30 122
MS.gene43540.t1 AT5G59450 26.178 382 269 6 55 431 226 599 7.02e-30 122
MS.gene43540.t1 AT4G08250 27.047 403 261 16 40 429 94 476 4.90e-24 104
MS.gene43540.t1 AT4G00150 23.664 393 233 14 58 430 209 554 1.94e-13 72.8
MS.gene43540.t1 AT3G60630 24.919 309 199 12 136 430 333 622 4.13e-12 68.6

Find 115 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGGCTGATTTAAATTT+TGG 0.142361 5.3:-8428501 MS.gene43540:CDS
TGAATAACGTTGAATTTAAA+TGG 0.226774 5.3:+8428711 None:intergenic
GATTTCACCTTTGAATTAAC+TGG 0.256797 5.3:-8429263 MS.gene43540:CDS
AAGAGTGTTTGAGATGGTTT+AGG 0.270052 5.3:-8428446 MS.gene43540:CDS
GGCTTTGTTGAGGAGGTATA+AGG 0.272310 5.3:-8428208 MS.gene43540:CDS
TTCGGTCATTCGAGAGAATA+AGG 0.284815 5.3:+8429091 None:intergenic
ACCTTCGAAGGCTTCTAAAA+TGG 0.301815 5.3:+8428932 None:intergenic
GGGGTGTGAGTGATGGTGTG+GGG 0.332972 5.3:+8429300 None:intergenic
TGGGAGAGGGATAGTTGATT+TGG 0.342461 5.3:-8428352 MS.gene43540:CDS
GGGTGTGAGTGATGGTGTGG+GGG 0.342781 5.3:+8429301 None:intergenic
AAATCAGTTGTTGGTGGAGG+GGG 0.353733 5.3:+8429281 None:intergenic
AGGAAGTTTGTGATGATGTT+AGG 0.369294 5.3:-8428230 MS.gene43540:CDS
AGAGTGTTTGAGATGGTTTA+GGG 0.380120 5.3:-8428445 MS.gene43540:CDS
GAAGGTTCTGGTACCGGCTT+CGG 0.382427 5.3:+8429073 None:intergenic
GAGGTTTGAGGTTGACTATA+AGG 0.384294 5.3:+8429344 None:intergenic
CGTTGATCGCGTTGCGGCCG+AGG 0.391480 5.3:-8428542 MS.gene43540:CDS
AGCTAGGGTTCGGAAGGTTC+TGG 0.400190 5.3:+8429061 None:intergenic
CTTCCACGTCAAGAATATTC+CGG 0.409628 5.3:+8428155 None:intergenic
GTTAGTCCCTGGACTACTTT+CGG 0.410539 5.3:-8428975 MS.gene43540:CDS
GTTTGTAGTAATGGTGTTTG+AGG 0.420379 5.3:+8429325 None:intergenic
ACTATAAGGATAGTAGTGTT+TGG 0.430455 5.3:+8429358 None:intergenic
GAAGGAAATTGGTGCTAGAA+TGG 0.434419 5.3:-8428760 MS.gene43540:CDS
AACGAACTAAGCTCCCCTTA+TGG 0.445036 5.3:-8429152 MS.gene43540:CDS
GTAATGGTGTTTGAGGTTTG+AGG 0.447709 5.3:+8429332 None:intergenic
TTCTCTCGAATGACCGAAGC+CGG 0.449633 5.3:-8429086 MS.gene43540:CDS
TCACGGCGTTTGCATGGTGG+AGG 0.454306 5.3:-8428276 MS.gene43540:CDS
TTGATGCTTGAAAGGGAAGC+TGG 0.455749 5.3:-8428372 MS.gene43540:CDS
CAGTGACGATGGTGGTTAAA+CGG 0.455896 5.3:+8428808 None:intergenic
ATGTTAGGGCTTTGTTGAGG+AGG 0.458311 5.3:-8428215 MS.gene43540:CDS
TTTGTTCTATGTCACCATAA+GGG 0.462505 5.3:+8429138 None:intergenic
CTATAGCTCGTGCTGTTTCG+AGG 0.464786 5.3:+8429219 None:intergenic
CGGTGGTGTGGGCCAGTGCA+TGG 0.470323 5.3:-8428125 MS.gene43540:CDS
TGAAATCAGTTGTTGGTGGA+GGG 0.471301 5.3:+8429279 None:intergenic
CATTCAGTAACAGTTGGTAA+TGG 0.475130 5.3:-8428600 MS.gene43540:CDS
CATGCACTGGCCCACACCAC+CGG 0.476107 5.3:+8428126 None:intergenic
GTGAAATCAGTTGTTGGTGG+AGG 0.476494 5.3:+8429278 None:intergenic
GGAAGTTTGTGATGATGTTA+GGG 0.478484 5.3:-8428229 MS.gene43540:CDS
CACGGCGTTTGCATGGTGGA+GGG 0.480736 5.3:-8428275 MS.gene43540:CDS
AAACGCTCGTTACTCGTCTT+CGG 0.484291 5.3:+8428393 None:intergenic
ATGGTTTAGGGTTTACTTTG+AGG 0.492134 5.3:-8428433 MS.gene43540:CDS
GATGGATATGAATTTGTGAA+TGG 0.492688 5.3:-8428474 MS.gene43540:CDS
GACATGGCCGAAAGTAGTCC+AGG 0.499248 5.3:+8428968 None:intergenic
TCAAAGGTGAAATCAGTTGT+TGG 0.501730 5.3:+8429272 None:intergenic
TGTTTCGAGGAGGATGTTGT+TGG 0.505657 5.3:+8429232 None:intergenic
CCACTTTCCAGTTAATTCAA+AGG 0.510883 5.3:+8429256 None:intergenic
GAAATCAGTTGTTGGTGGAG+GGG 0.511614 5.3:+8429280 None:intergenic
GCATGGTGGAGGGTTTAATA+CGG 0.512491 5.3:-8428265 MS.gene43540:CDS
CTGATGCTGAAGCTAGGGTT+CGG 0.515216 5.3:+8429051 None:intergenic
TTCGGCCATGTCGCATGCAA+TGG 0.515651 5.3:-8428957 MS.gene43540:CDS
CCTTTGAATTAACTGGAAAG+TGG 0.516151 5.3:-8429256 MS.gene43540:CDS
TGATTTGGTGGCATGTGCTC+CGG 0.516469 5.3:-8428337 MS.gene43540:CDS
TTATCAAGGCCTCCATGCAC+TGG 0.517596 5.3:+8428113 None:intergenic
GGAAGGAACAACCGGTGGTG+TGG 0.520705 5.3:-8428137 MS.gene43540:CDS
GAGAGGGATAGTTGATTTGG+TGG 0.522738 5.3:-8428349 MS.gene43540:CDS
CTTGACGTGGAAGGAACAAC+CGG 0.523785 5.3:-8428145 MS.gene43540:CDS
AGGAAGGGTGGTCGATGATA+CGG 0.526878 5.3:-8428188 MS.gene43540:CDS
CTCCGTTCAATCGACTCTGC+CGG 0.527978 5.3:+8428318 None:intergenic
ACGAGCGTTTGATGCTTGAA+AGG 0.528089 5.3:-8428380 MS.gene43540:CDS
GGTTCGGAAGGTTCTGGTAC+CGG 0.529243 5.3:+8429067 None:intergenic
ATTCAACGTTATTCATCACT+CGG 0.529452 5.3:-8428703 MS.gene43540:CDS
TAGTCCAGGGACTAACCTCT+TGG 0.534652 5.3:+8428982 None:intergenic
GAAGGAACAACCGGTGGTGT+GGG 0.535300 5.3:-8428136 MS.gene43540:CDS
GTCGATTGAACGGAGAGAAA+CGG 0.535584 5.3:-8428310 MS.gene43540:CDS
TCAACCACTGTTACTATCCT+CGG 0.540676 5.3:+8428525 None:intergenic
TTGTTCTATGTCACCATAAG+GGG 0.542773 5.3:+8429139 None:intergenic
CTTGAAAGGGAAGCTGGGAG+AGG 0.543069 5.3:-8428366 MS.gene43540:CDS
CGGAATATTCTTGACGTGGA+AGG 0.543303 5.3:-8428154 MS.gene43540:CDS
CGAGCGTTTGATGCTTGAAA+GGG 0.543796 5.3:-8428379 MS.gene43540:CDS
GGAGAGAAACGGCGGTGAGA+TGG 0.549109 5.3:-8428299 MS.gene43540:CDS
CAACTTGGAATCACCTTCGA+AGG 0.551692 5.3:+8428920 None:intergenic
TTAACCACCATCGTCACTGC+TGG 0.551969 5.3:-8428804 MS.gene43540:CDS
GATAGTAACAGTGGTTGAAG+AGG 0.553960 5.3:-8428520 MS.gene43540:CDS
GCTTTGCATTCAGTAACAGT+TGG 0.557029 5.3:-8428606 MS.gene43540:CDS
GATATCAATTATGTGCAACT+TGG 0.559173 5.3:+8428905 None:intergenic
AGATGGTCACGGCGTTTGCA+TGG 0.559260 5.3:-8428282 MS.gene43540:CDS
CTTTGAATTAACTGGAAAGT+GGG 0.560664 5.3:-8429255 MS.gene43540:CDS
AATTCCAAGAGGTTAGTCCC+TGG 0.573670 5.3:-8428986 MS.gene43540:CDS
TGCTGAAGCTAGGGTTCGGA+AGG 0.575914 5.3:+8429055 None:intergenic
ATGATACGGTGTAATGATGC+CGG 0.576413 5.3:-8428174 MS.gene43540:CDS
TGGCGCCATTGCATGCGACA+TGG 0.579732 5.3:+8428952 None:intergenic
ACCACCATCGTCACTGCTGG+TGG 0.581472 5.3:-8428801 MS.gene43540:CDS
AGTAACAGTGGTTGAAGAGG+AGG 0.584206 5.3:-8428517 MS.gene43540:CDS
CTCCGGCAGAGTCGATTGAA+CGG 0.585476 5.3:-8428320 MS.gene43540:CDS
TGATGCTTGAAAGGGAAGCT+GGG 0.585745 5.3:-8428371 MS.gene43540:CDS
TGTTGAGGAGGTATAAGGAA+GGG 0.588341 5.3:-8428203 MS.gene43540:CDS
TTGTTGAGGAGGTATAAGGA+AGG 0.589974 5.3:-8428204 MS.gene43540:CDS
CGGAGTATCATCAGCACGAG+TGG 0.595472 5.3:+8428836 None:intergenic
GTAACAGTTGGTAATGGTAA+CGG 0.598007 5.3:-8428594 MS.gene43540:CDS
AAGGTGAAATCAGTTGTTGG+TGG 0.600375 5.3:+8429275 None:intergenic
TCATTCGAGAGAATAAGGCT+TGG 0.601622 5.3:+8429096 None:intergenic
TTGAAAGGGAAGCTGGGAGA+GGG 0.603420 5.3:-8428365 MS.gene43540:CDS
TTCAACGTTATTCATCACTC+GGG 0.607328 5.3:-8428702 MS.gene43540:CDS
ATGATGTTAGGGCTTTGTTG+AGG 0.607674 5.3:-8428218 MS.gene43540:CDS
CTCGTTTACAACAACTCATG+TGG 0.608017 5.3:-8429181 MS.gene43540:CDS
GTTTGGAAGAAGATGATGAA+AGG 0.615269 5.3:+8429375 None:intergenic
AAGCTATCAACGCGTCGCGA+CGG 0.617261 5.3:+8428565 None:intergenic
TGAGGAGGTATAAGGAAGGG+TGG 0.622191 5.3:-8428200 MS.gene43540:CDS
TAGCTTCGTTGATCGCGTTG+CGG 0.632402 5.3:-8428548 MS.gene43540:CDS
TGGTCACGGCGTTTGCATGG+TGG 0.640251 5.3:-8428279 MS.gene43540:CDS
AAACGGCGGTGAGATGGTCA+CGG 0.645102 5.3:-8428293 MS.gene43540:CDS
TAATACGGTGGCGTTTAGTG+AGG 0.648084 5.3:-8428250 MS.gene43540:CDS
AACAGTCCTAAAATTCCAAG+AGG 0.649327 5.3:-8428997 MS.gene43540:CDS
AGAACCACCAGCAGTGACGA+TGG 0.652328 5.3:+8428797 None:intergenic
TGGTGGAGGGTTTAATACGG+TGG 0.662791 5.3:-8428262 MS.gene43540:CDS
GTAATACTTATTGCACTCAG+TGG 0.666806 5.3:-8428881 MS.gene43540:CDS
ATGCCGGAATATTCTTGACG+TGG 0.670161 5.3:-8428158 MS.gene43540:CDS
ATGGTGGTTAAACGGAGGTG+CGG 0.674756 5.3:+8428816 None:intergenic
TAGCTCGTGCTGTTTCGAGG+AGG 0.677191 5.3:+8429222 None:intergenic
ACATGGCCGAAAGTAGTCCA+GGG 0.682120 5.3:+8428969 None:intergenic
TGGTGTGGGCCAGTGCATGG+AGG 0.695562 5.3:-8428122 MS.gene43540:CDS
GACGTGGAAGGAACAACCGG+TGG 0.699506 5.3:-8428142 MS.gene43540:CDS
GCGGCCGAGGATAGTAACAG+TGG 0.704672 5.3:-8428529 MS.gene43540:CDS
ACCACCAGCAGTGACGATGG+TGG 0.722652 5.3:+8428800 None:intergenic
GATTGAACGGAGAGAAACGG+CGG 0.736491 5.3:-8428307 MS.gene43540:CDS
TGACGATGGTGGTTAAACGG+AGG 0.736521 5.3:+8428811 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTCTGATTTAAATTTTT+TGG - chr5.3:8428829-8428848 MS.gene43540:CDS 15.0%
!! TGAATAACGTTGAATTTAAA+TGG + chr5.3:8428797-8428816 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG - chr5.3:8429013-8429032 MS.gene43540:CDS 20.0%
! CAAAAAGTTATGAAGGAAAT+TGG - chr5.3:8428734-8428753 MS.gene43540:CDS 25.0%
! TTCTGTTCAAAAAGTTATGA+AGG - chr5.3:8428727-8428746 MS.gene43540:CDS 25.0%
ACTATAAGGATAGTAGTGTT+TGG + chr5.3:8428150-8428169 None:intergenic 30.0%
ATTCAACGTTATTCATCACT+CGG - chr5.3:8428802-8428821 MS.gene43540:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG - chr5.3:8428250-8428269 MS.gene43540:CDS 30.0%
GAAAAATTCGCTAGACTTAT+GGG - chr5.3:8428767-8428786 MS.gene43540:CDS 30.0%
GATATCAATTATGTGCAACT+TGG + chr5.3:8428603-8428622 None:intergenic 30.0%
GATGGATATGAATTTGTGAA+TGG - chr5.3:8429031-8429050 MS.gene43540:CDS 30.0%
GATTTCACCTTTGAATTAAC+TGG - chr5.3:8428242-8428261 MS.gene43540:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG + chr5.3:8428370-8428389 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG + chr5.3:8428371-8428390 None:intergenic 30.0%
AACAGTCCTAAAATTCCAAG+AGG - chr5.3:8428508-8428527 MS.gene43540:CDS 35.0%
CCACTTTCCAGTTAATTCAA+AGG + chr5.3:8428252-8428271 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG - chr5.3:8428249-8428268 MS.gene43540:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG - chr5.3:8428766-8428785 MS.gene43540:CDS 35.0%
GTAATACTTATTGCACTCAG+TGG - chr5.3:8428624-8428643 MS.gene43540:CDS 35.0%
GTTTGGAAGAAGATGATGAA+AGG + chr5.3:8428133-8428152 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG + chr5.3:8428183-8428202 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG + chr5.3:8428236-8428255 None:intergenic 35.0%
TTCAACGTTATTCATCACTC+GGG - chr5.3:8428803-8428822 MS.gene43540:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG + chr5.3:8428369-8428388 None:intergenic 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG - chr5.3:8429060-8429079 MS.gene43540:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG - chr5.3:8429275-8429294 MS.gene43540:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG - chr5.3:8429072-8429091 MS.gene43540:CDS 35.0%
! GACTAACCTCTTGGAATTTT+AGG + chr5.3:8428517-8428536 None:intergenic 35.0%
! GGAAGTTTGTGATGATGTTA+GGG - chr5.3:8429276-8429295 MS.gene43540:CDS 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG + chr5.3:8428462-8428481 None:intergenic 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG - chr5.3:8429059-8429078 MS.gene43540:CDS 35.0%
!! CATTCAGTAACAGTTGGTAA+TGG - chr5.3:8428905-8428924 MS.gene43540:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG - chr5.3:8429004-8429023 MS.gene43540:CDS 35.0%
!! GTAACAGTTGGTAATGGTAA+CGG - chr5.3:8428911-8428930 MS.gene43540:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG - chr5.3:8429053-8429072 MS.gene43540:CDS 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG + chr5.3:8428463-8428482 None:intergenic 35.0%
AAGGTGAAATCAGTTGTTGG+TGG + chr5.3:8428233-8428252 None:intergenic 40.0%
ATGATACGGTGTAATGATGC+CGG - chr5.3:8429331-8429350 MS.gene43540:CDS 40.0%
CTCGTTTACAACAACTCATG+TGG - chr5.3:8428324-8428343 MS.gene43540:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG + chr5.3:8429353-8429372 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG - chr5.3:8428985-8429004 MS.gene43540:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG - chr5.3:8428899-8428918 MS.gene43540:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG + chr5.3:8428983-8429002 None:intergenic 40.0%
TCATTCGAGAGAATAAGGCT+TGG + chr5.3:8428412-8428431 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG + chr5.3:8428417-8428436 None:intergenic 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG + chr5.3:8428576-8428595 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG + chr5.3:8428164-8428183 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG + chr5.3:8428176-8428195 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG + chr5.3:8428229-8428248 None:intergenic 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG - chr5.3:8429302-8429321 MS.gene43540:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG - chr5.3:8429301-8429320 MS.gene43540:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG - chr5.3:8429287-8429306 MS.gene43540:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG - chr5.3:8428745-8428764 MS.gene43540:CDS 40.0%
AAACGCTCGTTACTCGTCTT+CGG + chr5.3:8429115-8429134 None:intergenic 45.0%
AACGAACTAAGCTCCCCTTA+TGG - chr5.3:8428353-8428372 MS.gene43540:CDS 45.0%
ACGAGCGTTTGATGCTTGAA+AGG - chr5.3:8429125-8429144 MS.gene43540:CDS 45.0%
AGTAACAGTGGTTGAAGAGG+AGG - chr5.3:8428988-8429007 MS.gene43540:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG - chr5.3:8429347-8429366 MS.gene43540:CDS 45.0%
CAACTTGGAATCACCTTCGA+AGG + chr5.3:8428588-8428607 None:intergenic 45.0%
CGAGCGTTTGATGCTTGAAA+GGG - chr5.3:8429126-8429145 MS.gene43540:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG - chr5.3:8429351-8429370 MS.gene43540:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG - chr5.3:8429195-8429214 MS.gene43540:CDS 45.0%
GTTAGTCCCTGGACTACTTT+CGG - chr5.3:8428530-8428549 MS.gene43540:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG - chr5.3:8429255-8429274 MS.gene43540:CDS 45.0%
TGATGCTTGAAAGGGAAGCT+GGG - chr5.3:8429134-8429153 MS.gene43540:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG - chr5.3:8429153-8429172 MS.gene43540:CDS 45.0%
TGTTTCGAGGAGGATGTTGT+TGG + chr5.3:8428276-8428295 None:intergenic 45.0%
TTGATGCTTGAAAGGGAAGC+TGG - chr5.3:8429133-8429152 MS.gene43540:CDS 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG + chr5.3:8428227-8428246 None:intergenic 45.0%
! AATTCCAAGAGGTTAGTCCC+TGG - chr5.3:8428519-8428538 MS.gene43540:CDS 45.0%
! CAGTGACGATGGTGGTTAAA+CGG + chr5.3:8428700-8428719 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG + chr5.3:8428228-8428247 None:intergenic 45.0%
! GCATGGTGGAGGGTTTAATA+CGG - chr5.3:8429240-8429259 MS.gene43540:CDS 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG + chr5.3:8428230-8428249 None:intergenic 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG - chr5.3:8429290-8429309 MS.gene43540:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG - chr5.3:8429156-8429175 MS.gene43540:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG - chr5.3:8428572-8428591 MS.gene43540:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG - chr5.3:8429297-8429316 MS.gene43540:CDS 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG + chr5.3:8428192-8428211 None:intergenic 45.0%
ACATGGCCGAAAGTAGTCCA+GGG + chr5.3:8428539-8428558 None:intergenic 50.0%
AGGAAGGGTGGTCGATGATA+CGG - chr5.3:8429317-8429336 MS.gene43540:CDS 50.0%
ATGGTGGTTAAACGGAGGTG+CGG + chr5.3:8428692-8428711 None:intergenic 50.0%
CTGATGCTGAAGCTAGGGTT+CGG + chr5.3:8428457-8428476 None:intergenic 50.0%
CTTGACGTGGAAGGAACAAC+CGG - chr5.3:8429360-8429379 MS.gene43540:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG - chr5.3:8429198-8429217 MS.gene43540:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG - chr5.3:8428957-8428976 MS.gene43540:CDS 50.0%
TAGTCCAGGGACTAACCTCT+TGG + chr5.3:8428526-8428545 None:intergenic 50.0%
TGGTGGAGGGTTTAATACGG+TGG - chr5.3:8429243-8429262 MS.gene43540:CDS 50.0%
TTAACCACCATCGTCACTGC+TGG - chr5.3:8428701-8428720 MS.gene43540:CDS 50.0%
TTCTCTCGAATGACCGAAGC+CGG - chr5.3:8428419-8428438 MS.gene43540:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG - chr5.3:8429140-8429159 MS.gene43540:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG + chr5.3:8428650-8428669 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG + chr5.3:8428697-8428716 None:intergenic 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG - chr5.3:8429305-8429324 MS.gene43540:CDS 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG + chr5.3:8428289-8428308 None:intergenic 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG - chr5.3:8429168-8429187 MS.gene43540:CDS 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG - chr5.3:8428646-8428665 MS.gene43540:CDS 50.0%
AAACGGCGGTGAGATGGTCA+CGG - chr5.3:8429212-8429231 MS.gene43540:CDS 55.0%
AAGCTATCAACGCGTCGCGA+CGG + chr5.3:8428943-8428962 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG + chr5.3:8428711-8428730 None:intergenic 55.0%
AGATGGTCACGGCGTTTGCA+TGG - chr5.3:8429223-8429242 MS.gene43540:CDS 55.0%
AGCTAGGGTTCGGAAGGTTC+TGG + chr5.3:8428447-8428466 None:intergenic 55.0%
CTCCGGCAGAGTCGATTGAA+CGG - chr5.3:8429185-8429204 MS.gene43540:CDS 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG - chr5.3:8429139-8429158 MS.gene43540:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG - chr5.3:8429369-8429388 MS.gene43540:CDS 55.0%
GACATGGCCGAAAGTAGTCC+AGG + chr5.3:8428540-8428559 None:intergenic 55.0%
TGCTGAAGCTAGGGTTCGGA+AGG + chr5.3:8428453-8428472 None:intergenic 55.0%
TTCGGCCATGTCGCATGCAA+TGG - chr5.3:8428548-8428567 MS.gene43540:CDS 55.0%
! CGGAGTATCATCAGCACGAG+TGG + chr5.3:8428672-8428691 None:intergenic 55.0%
! CTCCGTTCAATCGACTCTGC+CGG + chr5.3:8429190-8429209 None:intergenic 55.0%
! GGTTCGGAAGGTTCTGGTAC+CGG + chr5.3:8428441-8428460 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG + chr5.3:8428435-8428454 None:intergenic 55.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG + chr5.3:8428286-8428305 None:intergenic 55.0%
ACCACCAGCAGTGACGATGG+TGG + chr5.3:8428708-8428727 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG - chr5.3:8428704-8428723 MS.gene43540:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG - chr5.3:8429363-8429382 MS.gene43540:CDS 60.0%
GCGGCCGAGGATAGTAACAG+TGG - chr5.3:8428976-8428995 MS.gene43540:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG - chr5.3:8429368-8429387 MS.gene43540:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG - chr5.3:8429206-8429225 MS.gene43540:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG - chr5.3:8429229-8429248 MS.gene43540:CDS 60.0%
TGGCGCCATTGCATGCGACA+TGG + chr5.3:8428556-8428575 None:intergenic 60.0%
TGGTCACGGCGTTTGCATGG+TGG - chr5.3:8429226-8429245 MS.gene43540:CDS 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG + chr5.3:8428210-8428229 None:intergenic 60.0%
! CACGGCGTTTGCATGGTGGA+GGG - chr5.3:8429230-8429249 MS.gene43540:CDS 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG + chr5.3:8428209-8428228 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG + chr5.3:8428208-8428227 None:intergenic 60.0%
! GGGTGTGAGTGATGGTGTGG+GGG + chr5.3:8428207-8428226 None:intergenic 60.0%
TGGTGTGGGCCAGTGCATGG+AGG - chr5.3:8429383-8429402 MS.gene43540:CDS 65.0%
! CATGCACTGGCCCACACCAC+CGG + chr5.3:8429382-8429401 None:intergenic 65.0%
! GGTGGAGGGGGTGTGAGTGA+TGG + chr5.3:8428215-8428234 None:intergenic 65.0%
CGGTGGTGTGGGCCAGTGCA+TGG - chr5.3:8429380-8429399 MS.gene43540:CDS 70.0%
CGTTGATCGCGTTGCGGCCG+AGG - chr5.3:8428963-8428982 MS.gene43540:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 8428116 8429411 8428116 ID=MS.gene43540
chr5.3 mRNA 8428116 8429411 8428116 ID=MS.gene43540.t1;Parent=MS.gene43540
chr5.3 exon 8428116 8429411 8428116 ID=MS.gene43540.t1.exon1;Parent=MS.gene43540.t1
chr5.3 CDS 8428116 8429411 8428116 ID=cds.MS.gene43540.t1;Parent=MS.gene43540.t1
Gene Sequence

>MS.gene43540

ATGGATGAAGAAGACCTTTCATCATCTTCTTCCAAACACTACTATCCTTATAGTCAACCTCAAACCTCAAACACCATTACTACAAACACCCCCACACCATCACTCACACCCCCTCCACCAACAACTGATTTCACCTTTGAATTAACTGGAAAGTGGGCCAACAACATCCTCCTCGAAACAGCACGAGCTATAGCAGAAAAAAACAGCACTCGTTTACAACAACTCATGTGGATGCTCAACGAACTAAGCTCCCCTTATGGTGACATAGAACAAAAACTAGCTGCATATTTTCTCCAAGCCTTATTCTCTCGAATGACCGAAGCCGGTACCAGAACCTTCCGAACCCTAGCTTCAGCATCAGAAAAAACTTGCTCATTCGAGTCAACAAGAAAAACAGTCCTAAAATTCCAAGAGGTTAGTCCCTGGACTACTTTCGGCCATGTCGCATGCAATGGCGCCATTTTAGAAGCCTTCGAAGGTGATTCCAAGTTGCACATAATTGATATCAGTAATACTTATTGCACTCAGTGGCCAACGCTTTTTGAAGCGTTGGCCACTCGTGCTGATGATACTCCGCACCTCCGTTTAACCACCATCGTCACTGCTGGTGGTTCTGTTCAAAAAGTTATGAAGGAAATTGGTGCTAGAATGGAAAAATTCGCTAGACTTATGGGAGTTCCATTTAAATTCAACGTTATTCATCACTCGGGCGATCTTTCTGATTTAAATTTTTTGGATTTAGATATTAAAGAAGATGAAGCTTTAGCTGTTAATTGTGTTAATGCTTTGCATTCAGTAACAGTTGGTAATGGTAACGGTAATAACCGTCGCGACGCGTTGATAGCTTCGTTGATCGCGTTGCGGCCGAGGATAGTAACAGTGGTTGAAGAGGAGGCTGATTTAAATTTTGGTAATGATGGATATGAATTTGTGAATGGTTTTGAAGAGTGTTTGAGATGGTTTAGGGTTTACTTTGAGGCATTAGAAGAGAGTTTTCCGAAGACGAGTAACGAGCGTTTGATGCTTGAAAGGGAAGCTGGGAGAGGGATAGTTGATTTGGTGGCATGTGCTCCGGCAGAGTCGATTGAACGGAGAGAAACGGCGGTGAGATGGTCACGGCGTTTGCATGGTGGAGGGTTTAATACGGTGGCGTTTAGTGAGGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGGAGGTATAAGGAAGGGTGGTCGATGATACGGTGTAATGATGCCGGAATATTCTTGACGTGGAAGGAACAACCGGTGGTGTGGGCCAGTGCATGGAGGCCTTGA
Protein sequence

>MS.gene43540.t1

MDEEDLSSSSSKHYYPYSQPQTSNTITTNTPTPSLTPPPPTTDFTFELTGKWANNILLETARAIAEKNSTRLQQLMWMLNELSSPYGDIEQKLAAYFLQALFSRMTEAGTRTFRTLASASEKTCSFESTRKTVLKFQEVSPWTTFGHVACNGAILEAFEGDSKLHIIDISNTYCTQWPTLFEALATRADDTPHLRLTTIVTAGGSVQKVMKEIGARMEKFARLMGVPFKFNVIHHSGDLSDLNFLDLDIKEDEALAVNCVNALHSVTVGNGNGNNRRDALIASLIALRPRIVTVVEEEADLNFGNDGYEFVNGFEECLRWFRVYFEALEESFPKTSNERLMLEREAGRGIVDLVACAPAESIERRETAVRWSRRLHGGGFNTVAFSEEVCDDVRALLRRYKEGWSMIRCNDAGIFLTWKEQPVVWASAWRP