Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43762.t1 | OMJ84645.1 | 40 | 95 | 52 | 2 | 22 | 116 | 44 | 133 | 3.80E-07 | 64.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43762.t1 | Q9FKI4 | 43.0 | 93 | 47 | 3 | 22 | 114 | 44 | 130 | 1.3e-08 | 60.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43762.t1 | A0A1R2C6T9 | 40.0 | 95 | 52 | 2 | 22 | 116 | 44 | 133 | 2.7e-07 | 64.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene43762.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43762.t1 | MTR_3g034200 | 40.625 | 96 | 51 | 3 | 21 | 116 | 43 | 132 | 5.62e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43762.t1 | AT5G13780 | 42.553 | 94 | 48 | 3 | 21 | 114 | 43 | 130 | 4.00e-12 | 60.8 |
Find 32 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCGTAATCCAAGGCCTTT+TGG | 0.266555 | 5.1:+54180312 | None:intergenic |
| AAACCTGTTCCAAATCCTTA+TGG | 0.271438 | 5.1:+54180643 | None:intergenic |
| CTTATCTTGCCTTTGAGTAC+TGG | 0.344775 | 5.1:-54180849 | MS.gene43762:CDS |
| GACTGCCGCCCATAAGGATT+TGG | 0.362443 | 5.1:-54180652 | MS.gene43762:CDS |
| CGCCCATAAGGATTTGGAAC+AGG | 0.390119 | 5.1:-54180646 | MS.gene43762:intron |
| GAAGGAGGAGGAGGTTAAGA+AGG | 0.410903 | 5.1:-54180754 | MS.gene43762:CDS |
| AACCTGTTCCAAATCCTTAT+GGG | 0.426781 | 5.1:+54180644 | None:intergenic |
| TTTATAAGCCGATATCTTCT+CGG | 0.436779 | 5.1:-54180253 | MS.gene43762:CDS |
| GCTTGTGACTGCCGCCCATA+AGG | 0.446228 | 5.1:-54180658 | MS.gene43762:CDS |
| TTATAAGCCGATATCTTCTC+GGG | 0.458239 | 5.1:-54180252 | MS.gene43762:CDS |
| GATTACGATATTTATGAAGC+TGG | 0.472558 | 5.1:-54180299 | MS.gene43762:CDS |
| TTGGTCTCCCGAGAAGATAT+CGG | 0.486784 | 5.1:+54180245 | None:intergenic |
| CTCTATTAAGAAGGAGGAGG+AGG | 0.487768 | 5.1:-54180763 | MS.gene43762:CDS |
| GGAGGAGGAGGTTAAGAAGG+AGG | 0.522170 | 5.1:-54180751 | MS.gene43762:CDS |
| AAGCTGGAATGTTGTACCTC+GGG | 0.522913 | 5.1:+54180345 | None:intergenic |
| CTCGCAACCTATCAGAGACT+TGG | 0.530721 | 5.1:-54180693 | MS.gene43762:CDS |
| TTATGTCTGCTCTATTAAGA+AGG | 0.559468 | 5.1:-54180772 | MS.gene43762:CDS |
| AGCGATACCAAGTCTCTGAT+AGG | 0.565478 | 5.1:+54180686 | None:intergenic |
| TTCATAAATATCGTAATCCA+AGG | 0.569142 | 5.1:+54180304 | None:intergenic |
| GCAAGATAAGATCATATTCG+CGG | 0.579984 | 5.1:+54180862 | None:intergenic |
| TTTCACGTCGCGGTCAGGAA+CGG | 0.583681 | 5.1:-54180816 | MS.gene43762:CDS |
| CTGCTCTATTAAGAAGGAGG+AGG | 0.589794 | 5.1:-54180766 | MS.gene43762:CDS |
| CACGTCGCGGTCAGGAACGG+CGG | 0.594718 | 5.1:-54180813 | MS.gene43762:CDS |
| CGCTGAAATCAGTCACGTCG+TGG | 0.599192 | 5.1:-54180718 | MS.gene43762:CDS |
| TGTTCATCTCCAGTACTCAA+AGG | 0.610835 | 5.1:+54180840 | None:intergenic |
| GGCGGCCGTCGCAATATCAT+CGG | 0.612517 | 5.1:-54180795 | MS.gene43762:CDS |
| AAAGCTGGAATGTTGTACCT+CGG | 0.618480 | 5.1:+54180344 | None:intergenic |
| ACATCTCAGCGCTCGTCCCG+AGG | 0.619758 | 5.1:-54180361 | MS.gene43762:CDS |
| ACGAGCGCTGAGATGTACTC+AGG | 0.636447 | 5.1:+54180368 | None:intergenic |
| TGTCTGCTCTATTAAGAAGG+AGG | 0.648646 | 5.1:-54180769 | MS.gene43762:CDS |
| CTGTTCCAAATCCTTATGGG+CGG | 0.673914 | 5.1:+54180647 | None:intergenic |
| CATAACCGATGATATTGCGA+CGG | 0.693605 | 5.1:+54180790 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATATAGAATTACAGAACT+AGG | + | chr5.1:54180586-54180605 | None:intergenic | 25.0% |
| ! | CATATAGAATTACAGAACTA+GGG | + | chr5.1:54180585-54180604 | None:intergenic | 25.0% |
| ! | GTTCTGTAATTCTATATGTT+AGG | - | chr5.1:54180587-54180606 | MS.gene43762:intron | 25.0% |
| ! | TTCATAAATATCGTAATCCA+AGG | + | chr5.1:54180816-54180835 | None:intergenic | 25.0% |
| GATTACGATATTTATGAAGC+TGG | - | chr5.1:54180818-54180837 | MS.gene43762:CDS | 30.0% | |
| TTATGTCTGCTCTATTAAGA+AGG | - | chr5.1:54180345-54180364 | MS.gene43762:CDS | 30.0% | |
| TTTATAAGCCGATATCTTCT+CGG | - | chr5.1:54180864-54180883 | MS.gene43762:CDS | 30.0% | |
| ! | AGCTTTCATTTTGTTCCAAA+AGG | - | chr5.1:54180790-54180809 | MS.gene43762:CDS | 30.0% |
| !!! | TTTTGGAACAAAATGAAAGC+TGG | + | chr5.1:54180791-54180810 | None:intergenic | 30.0% |
| AAACCTGTTCCAAATCCTTA+TGG | + | chr5.1:54180477-54180496 | None:intergenic | 35.0% | |
| AACCTGTTCCAAATCCTTAT+GGG | + | chr5.1:54180476-54180495 | None:intergenic | 35.0% | |
| GCAAGATAAGATCATATTCG+CGG | + | chr5.1:54180258-54180277 | None:intergenic | 35.0% | |
| TTATAAGCCGATATCTTCTC+GGG | - | chr5.1:54180865-54180884 | MS.gene43762:CDS | 35.0% | |
| AAAGCTGGAATGTTGTACCT+CGG | + | chr5.1:54180776-54180795 | None:intergenic | 40.0% | |
| CATAACCGATGATATTGCGA+CGG | + | chr5.1:54180330-54180349 | None:intergenic | 40.0% | |
| CTTATCTTGCCTTTGAGTAC+TGG | - | chr5.1:54180268-54180287 | MS.gene43762:CDS | 40.0% | |
| TGTCTGCTCTATTAAGAAGG+AGG | - | chr5.1:54180348-54180367 | MS.gene43762:CDS | 40.0% | |
| TGTTCATCTCCAGTACTCAA+AGG | + | chr5.1:54180280-54180299 | None:intergenic | 40.0% | |
| ! | AGATGAACATTTTCACGTCG+CGG | - | chr5.1:54180291-54180310 | MS.gene43762:CDS | 40.0% |
| ! | CATTTTGTTCCAAAAGGCCT+TGG | - | chr5.1:54180796-54180815 | MS.gene43762:CDS | 40.0% |
| ! | TATCGTAATCCAAGGCCTTT+TGG | + | chr5.1:54180808-54180827 | None:intergenic | 40.0% |
| AAGCTGGAATGTTGTACCTC+GGG | + | chr5.1:54180775-54180794 | None:intergenic | 45.0% | |
| AGCGATACCAAGTCTCTGAT+AGG | + | chr5.1:54180434-54180453 | None:intergenic | 45.0% | |
| CTCTATTAAGAAGGAGGAGG+AGG | - | chr5.1:54180354-54180373 | MS.gene43762:CDS | 45.0% | |
| CTGCTCTATTAAGAAGGAGG+AGG | - | chr5.1:54180351-54180370 | MS.gene43762:CDS | 45.0% | |
| CTGTTCCAAATCCTTATGGG+CGG | + | chr5.1:54180473-54180492 | None:intergenic | 45.0% | |
| TTGGTCTCCCGAGAAGATAT+CGG | + | chr5.1:54180875-54180894 | None:intergenic | 45.0% | |
| CTCGCAACCTATCAGAGACT+TGG | - | chr5.1:54180424-54180443 | MS.gene43762:intron | 50.0% | |
| GAAGGAGGAGGAGGTTAAGA+AGG | - | chr5.1:54180363-54180382 | MS.gene43762:CDS | 50.0% | |
| ! | AACATTTTCACGTCGCGGTC+AGG | - | chr5.1:54180296-54180315 | MS.gene43762:CDS | 50.0% |
| ! | CGCCCATAAGGATTTGGAAC+AGG | - | chr5.1:54180471-54180490 | MS.gene43762:intron | 50.0% |
| CGCTGAAATCAGTCACGTCG+TGG | - | chr5.1:54180399-54180418 | MS.gene43762:intron | 55.0% | |
| GACTGCCGCCCATAAGGATT+TGG | - | chr5.1:54180465-54180484 | MS.gene43762:intron | 55.0% | |
| GGAGGAGGAGGTTAAGAAGG+AGG | - | chr5.1:54180366-54180385 | MS.gene43762:CDS | 55.0% | |
| TTTCACGTCGCGGTCAGGAA+CGG | - | chr5.1:54180301-54180320 | MS.gene43762:CDS | 55.0% | |
| !! | ACGAGCGCTGAGATGTACTC+AGG | + | chr5.1:54180752-54180771 | None:intergenic | 55.0% |
| GCTTGTGACTGCCGCCCATA+AGG | - | chr5.1:54180459-54180478 | MS.gene43762:intron | 60.0% | |
| GGCGGCCGTCGCAATATCAT+CGG | - | chr5.1:54180322-54180341 | MS.gene43762:CDS | 60.0% | |
| ACATCTCAGCGCTCGTCCCG+AGG | - | chr5.1:54180756-54180775 | MS.gene43762:CDS | 65.0% | |
| CACGTCGCGGTCAGGAACGG+CGG | - | chr5.1:54180304-54180323 | MS.gene43762:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 54180241 | 54180898 | 54180241 | ID=MS.gene43762 |
| chr5.1 | mRNA | 54180241 | 54180898 | 54180241 | ID=MS.gene43762.t1;Parent=MS.gene43762 |
| chr5.1 | exon | 54180647 | 54180898 | 54180647 | ID=MS.gene43762.t1.exon1;Parent=MS.gene43762.t1 |
| chr5.1 | CDS | 54180647 | 54180898 | 54180647 | ID=cds.MS.gene43762.t1;Parent=MS.gene43762.t1 |
| chr5.1 | exon | 54180241 | 54180399 | 54180241 | ID=MS.gene43762.t1.exon2;Parent=MS.gene43762.t1 |
| chr5.1 | CDS | 54180241 | 54180399 | 54180241 | ID=cds.MS.gene43762.t1;Parent=MS.gene43762.t1 |
| Gene Sequence |
| Protein sequence |
