AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43901

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43901.t1 MTR_5g035040 78.470 353 43 2 12 355 9 337 0.0 529
MS.gene43901.t1 MTR_5g035060 57.308 260 53 7 154 355 1 260 9.53e-77 239
MS.gene43901.t1 MTR_7g033775 73.333 135 33 2 5 137 15 148 4.15e-43 149
MS.gene43901.t1 MTR_4g105200 55.422 83 37 0 35 117 36 118 4.35e-24 99.0
MS.gene43901.t1 MTR_5g035050 87.755 49 6 0 307 355 29 77 2.13e-22 91.7
MS.gene43901.t1 MTR_6g005040 43.590 117 66 0 18 134 41 157 1.98e-16 77.4
MS.gene43901.t1 MTR_3g109190 35.135 111 71 1 27 137 24 133 4.86e-16 75.5
MS.gene43901.t1 MTR_1g090250 36.111 108 69 0 34 141 27 134 5.34e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43901.t1 AT1G24040 56.701 194 69 2 176 354 80 273 3.37e-67 216
MS.gene43901.t1 AT1G24040 56.701 194 69 2 176 354 80 273 3.37e-67 216
MS.gene43901.t1 AT1G24040 56.701 194 69 2 176 354 80 273 3.37e-67 216
MS.gene43901.t1 AT3G01570 43.885 139 75 3 8 146 7 142 2.95e-30 115
MS.gene43901.t1 AT3G27660 50.000 116 57 1 31 146 43 157 6.14e-21 90.1
MS.gene43901.t1 AT3G27660 50.000 116 57 1 31 146 43 157 6.14e-21 90.1
MS.gene43901.t1 AT5G40420 53.846 104 48 0 34 137 48 151 2.29e-19 85.9
MS.gene43901.t1 AT5G51210 39.773 88 53 0 30 117 17 104 2.59e-17 78.6
MS.gene43901.t1 AT4G25140 38.776 98 55 1 19 116 27 119 6.03e-14 70.1
MS.gene43901.t1 AT2G25890 44.828 87 48 0 31 117 44 130 3.03e-11 62.4
MS.gene43901.t1 AT2G25890 36.937 111 70 0 31 141 19 129 6.41e-11 60.5

Find 93 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAAATCCGGTTCAATTT+TGG 0.113243 5.2:+25474574 MS.gene43901:CDS
CTTGCTGGACTCTCCCTTCT+TGG 0.217986 5.2:+25474143 MS.gene43901:CDS
CCTGTAGAGGCCCTTGATCC+TGG 0.232255 5.2:-25474331 None:intergenic
ACATGTTGATATAACATTTC+GGG 0.273357 5.2:-25474673 None:intergenic
AACCAAAATTGAACCGGATT+TGG 0.284192 5.2:-25474576 None:intergenic
TTGAACCGGATTTGGTGTTT+TGG 0.312879 5.2:-25474568 None:intergenic
AACATGTTGATATAACATTT+CGG 0.331289 5.2:-25474674 None:intergenic
CAAGTGCTTGCCGTAATTAC+CGG 0.339584 5.2:+25474092 MS.gene43901:CDS
GTTCCAGCCACCATTGTCAT+TGG 0.345015 5.2:+25474224 MS.gene43901:CDS
TAATCAAATATTGTTTCATA+AGG 0.361459 5.2:-25474703 None:intergenic
TCTTGGAACACTCACTGGAC+TGG 0.377988 5.2:+25474160 MS.gene43901:CDS
GAGGACTCGACGAGTGGTTT+TGG 0.383378 5.2:-25474495 None:intergenic
CTTGGAACACTCACTGGACT+GGG 0.386557 5.2:+25474161 MS.gene43901:CDS
TGGCACTGTCCTTGTTCTCA+TGG 0.386698 5.2:+25474291 MS.gene43901:CDS
GGAAGGATTCGAGGTGTTGC+AGG 0.387564 5.2:-25474538 None:intergenic
GGTGTACTGCTTGCACTTGC+TGG 0.398295 5.2:+25474128 MS.gene43901:CDS
GTGTTGCAGGTTGGTTAGTT+CGG 0.403050 5.2:-25474525 None:intergenic
ATGGTGGCTGGAACAAGAAC+AGG 0.405324 5.2:-25474215 None:intergenic
TACTCGAACCAAAATTGAAC+CGG 0.409292 5.2:-25474582 None:intergenic
TACAGGAAGGCCGGTAATTA+CGG 0.409924 5.2:-25474102 None:intergenic
TCTGCGAGTAAACAGACTAT+TGG 0.411736 5.2:-25474626 None:intergenic
TTCGTCTCTGCAACATCAGC+AGG 0.418356 5.2:-25475913 None:intergenic
CTGTAGAGGCCCTTGATCCT+GGG 0.419299 5.2:-25474330 None:intergenic
CCTTAGGCCCTCAAAATCAT+TGG 0.420597 5.2:+25474462 MS.gene43901:CDS
TATTTGATTAAACGAAGGAT+TGG 0.433371 5.2:+25474716 MS.gene43901:CDS
ATTTGATTAAACGAAGGATT+GGG 0.434126 5.2:+25474717 MS.gene43901:CDS
TCCCTTCTTGGAACACTCAC+TGG 0.449866 5.2:+25474155 MS.gene43901:CDS
ATTCTTTCACTCTCCTCCTT+AGG 0.453455 5.2:+25474446 MS.gene43901:CDS
CTGCGAGTAAACAGACTATT+GGG 0.455560 5.2:-25474625 None:intergenic
GGAAGTAGAGGAAGGATTCG+AGG 0.455777 5.2:-25474547 None:intergenic
ATAAGCGTAAGGCGAATCCT+TGG 0.465649 5.2:-25474846 None:intergenic
ATTCCCTCCAGGAGGGGCAT+TGG 0.469054 5.2:+25475742 MS.gene43901:intron
TACTTCCAAAACACCAAATC+CGG 0.469207 5.2:+25474563 MS.gene43901:CDS
CAAGTGAGGAACTGATTTCT+CGG 0.471299 5.2:+25475782 MS.gene43901:CDS
CCGTGGATAAATCTCTTAGA+AGG 0.481490 5.2:+25474876 MS.gene43901:CDS
GGCACTGTCCTTGTTCTCAT+GGG 0.495063 5.2:+25474292 MS.gene43901:CDS
GCAAGCACTTGAGAAGTTGA+TGG 0.495981 5.2:-25474080 None:intergenic
CCAGGATCAAGGGCCTCTAC+AGG 0.506135 5.2:+25474331 MS.gene43901:CDS
TAAGCGTAAGGCGAATCCTT+GGG 0.511314 5.2:-25474845 None:intergenic
TCCAGTGAGTGTTCCAAGAA+GGG 0.516170 5.2:-25474156 None:intergenic
AATTACATACCCCAGGATCA+AGG 0.516525 5.2:+25474320 MS.gene43901:CDS
TTCGCCTTACGCTTATAGCT+TGG 0.517762 5.2:+25474853 MS.gene43901:CDS
TTTCTTCAGTTCTATTACCA+TGG 0.524414 5.2:+25474418 MS.gene43901:CDS
AGTTTACTTGCATTGCAGAA+TGG 0.524955 5.2:+25475822 MS.gene43901:CDS
GTCCAGTGAGTGTTCCAAGA+AGG 0.526230 5.2:-25474157 None:intergenic
TGCTTCACCCACTCCTCCCA+AGG 0.526357 5.2:+25474829 MS.gene43901:CDS
CCTCAACATCGTTATGAAGT+TGG 0.529388 5.2:+25474023 MS.gene43901:CDS
GGCTGAAGAGGATGAAAAGT+GGG 0.530470 5.2:-25474193 None:intergenic
AAGCCAATGACAATGGTGGC+TGG 0.533271 5.2:-25474227 None:intergenic
GAACAAGAACAGGGCTGAAG+AGG 0.534164 5.2:-25474205 None:intergenic
GGCGAATCCTTGGGAGGAGT+GGG 0.537228 5.2:-25474836 None:intergenic
GCAAGCAGTACACCGCCTAC+AGG 0.537513 5.2:-25474119 None:intergenic
TCTACAGGAGCAACTCGAGT+TGG 0.549387 5.2:+25474346 MS.gene43901:CDS
CAACTTCATAACGATGTTGA+GGG 0.550235 5.2:-25474022 None:intergenic
AGGCGAATCCTTGGGAGGAG+TGG 0.550564 5.2:-25474837 None:intergenic
AACAATATTTGATTAAACGA+AGG 0.558233 5.2:+25474711 MS.gene43901:CDS
CCTCCAGGAGGGGCATTGGA+CGG 0.559072 5.2:+25475746 MS.gene43901:intron
TTACGCTTATAGCTTGGCCG+TGG 0.563922 5.2:+25474859 MS.gene43901:CDS
AACTCGAGTTGCTCCTGTAG+AGG 0.565489 5.2:-25474344 None:intergenic
CAACATCGTTATGAAGTTGG+TGG 0.571137 5.2:+25474026 MS.gene43901:CDS
TTTGAAGGCATAGTTAGGAG+AGG 0.572561 5.2:-25475959 None:intergenic
GTTTGTGAGGACTCGACGAG+TGG 0.581088 5.2:-25474501 None:intergenic
TGTAGAGGCCCTTGATCCTG+GGG 0.582357 5.2:-25474329 None:intergenic
TGGTTAGTTCGGAGTTTGTG+AGG 0.583118 5.2:-25474514 None:intergenic
ATTACATACCCCAGGATCAA+GGG 0.590997 5.2:+25474321 MS.gene43901:CDS
GGATTCGAGGTGTTGCAGGT+TGG 0.596620 5.2:-25474534 None:intergenic
CCAACTTCATAACGATGTTG+AGG 0.597401 5.2:-25474023 None:intergenic
GAGTGAAAGAATTGCTGCCA+TGG 0.598782 5.2:-25474435 None:intergenic
TGGAACACTCACTGGACTGG+GGG 0.600169 5.2:+25474163 MS.gene43901:CDS
TACTCATAGTCATCGGAACA+TGG 0.601957 5.2:-25475992 None:intergenic
GGTGAAGAAATGCAGTTGGC+TGG 0.602521 5.2:+25474761 MS.gene43901:CDS
GCTTATGGTGGAGTGGTGGT+CGG 0.603978 5.2:+25474376 MS.gene43901:CDS
ATTCATGACCCATGAGAACA+AGG 0.605807 5.2:-25474300 None:intergenic
GGTCATGAATTACATACCCC+AGG 0.611661 5.2:+25474313 MS.gene43901:CDS
GTAATTACCGGCCTTCCTGT+AGG 0.612160 5.2:+25474104 MS.gene43901:CDS
TACACCGCCTACAGGAAGGC+CGG 0.615441 5.2:-25474111 None:intergenic
GGGCTGAAGAGGATGAAAAG+TGG 0.618573 5.2:-25474194 None:intergenic
AACAGCTTATGGTGGAGTGG+TGG 0.622508 5.2:+25474372 MS.gene43901:CDS
GACAGCAAAGCCAATGACAA+TGG 0.626525 5.2:-25474234 None:intergenic
CCTTCTAAGAGATTTATCCA+CGG 0.626878 5.2:-25474876 None:intergenic
ACGGCCAAGCTATAAGCGTA+AGG 0.627421 5.2:-25474857 None:intergenic
TGGTGGAGTGGTGGTCGGAG+TGG 0.638660 5.2:+25474381 MS.gene43901:CDS
GCAGTACACCGCCTACAGGA+AGG 0.642229 5.2:-25474115 None:intergenic
TTGGAACACTCACTGGACTG+GGG 0.646592 5.2:+25474162 MS.gene43901:CDS
ACGATGTTGAGGGGTAACAC+TGG 0.650096 5.2:-25474012 None:intergenic
TGATGGTGAAGAAATGCAGT+TGG 0.661675 5.2:+25474757 MS.gene43901:CDS
GCGTAAGGCGAATCCTTGGG+AGG 0.670081 5.2:-25474842 None:intergenic
AACTTCATAACGATGTTGAG+GGG 0.671058 5.2:-25474021 None:intergenic
AGCAAAGCCAATGACAATGG+TGG 0.674104 5.2:-25474231 None:intergenic
TGGTGGCTGGAACAAGAACA+GGG 0.708517 5.2:-25474214 None:intergenic
ATTACCGGCCTTCCTGTAGG+CGG 0.711293 5.2:+25474107 MS.gene43901:CDS
CTGGTATCGTAACAGCGTGG+TGG 0.712087 5.2:-25473993 None:intergenic
ACACTGGTATCGTAACAGCG+TGG 0.804350 5.2:-25473996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATATTGTTTCATA+AGG - chr5.2:25474706-25474725 None:intergenic 15.0%
!! AACAATATTTGATTAAACGA+AGG + chr5.2:25474711-25474730 MS.gene43901:CDS 20.0%
!! AACATGTTGATATAACATTT+CGG - chr5.2:25474677-25474696 None:intergenic 20.0%
!! AGCTTAATTATTTAAATTGC+AGG - chr5.2:25475569-25475588 None:intergenic 20.0%
!! TTTCGATTATTCAAAGATTA+AGG + chr5.2:25475057-25475076 MS.gene43901:intron 20.0%
!!! GAGATTTGTTTTGATAAAAA+TGG + chr5.2:25474791-25474810 MS.gene43901:CDS 20.0%
! ACATGTTGATATAACATTTC+GGG - chr5.2:25474676-25474695 None:intergenic 25.0%
! AGATTATGTAGGATATTTCT+TGG + chr5.2:25475434-25475453 MS.gene43901:intron 25.0%
! ATAAAGAACTAATTTGACCA+AGG + chr5.2:25474973-25474992 MS.gene43901:intron 25.0%
! ATAATAGCAACAAGAAAATG+TGG - chr5.2:25475270-25475289 None:intergenic 25.0%
! ATTTGATTAAACGAAGGATT+GGG + chr5.2:25474717-25474736 MS.gene43901:CDS 25.0%
! GATTATGTAGGATATTTCTT+GGG + chr5.2:25475435-25475454 MS.gene43901:intron 25.0%
! TAAAGAACTAATTTGACCAA+GGG + chr5.2:25474974-25474993 MS.gene43901:intron 25.0%
! TATTCTTGGTCTAATTTGAT+TGG + chr5.2:25475003-25475022 MS.gene43901:intron 25.0%
! TATTTGATTAAACGAAGGAT+TGG + chr5.2:25474716-25474735 MS.gene43901:CDS 25.0%
! TTTCACATATAGATACACTT+GGG - chr5.2:25475341-25475360 None:intergenic 25.0%
!! CTACAAACTGAAACATTTTT+GGG + chr5.2:25475121-25475140 MS.gene43901:intron 25.0%
!! TTGGTCAAATTAGTTCTTTA+TGG - chr5.2:25474974-25474993 None:intergenic 25.0%
AAATGCAAATTTCAGCAACT+TGG - chr5.2:25474921-25474940 None:intergenic 30.0%
AATAAGCTGAAATCAGCTTA+TGG - chr5.2:25475186-25475205 None:intergenic 30.0%
CCAAAAATGTTTCAGTTTGT+AGG - chr5.2:25475123-25475142 None:intergenic 30.0%
CTTTCACATATAGATACACT+TGG - chr5.2:25475342-25475361 None:intergenic 30.0%
GATTAAGGCTGTATTTAGAT+TGG + chr5.2:25475072-25475091 MS.gene43901:intron 30.0%
GGTTACTTGAATCTTAAACT+TGG - chr5.2:25475593-25475612 None:intergenic 30.0%
TGCTGAAATTTGCATTTCTT+TGG + chr5.2:25474924-25474943 MS.gene43901:intron 30.0%
TGTGTTCAAAAACTATCCTT+GGG - chr5.2:25475219-25475238 None:intergenic 30.0%
TGTTTGAAAATGTGAGCTAT+GGG - chr5.2:25475641-25475660 None:intergenic 30.0%
TTCACATATAGATACACTTG+GGG - chr5.2:25475340-25475359 None:intergenic 30.0%
TTGTGTTCAAAAACTATCCT+TGG - chr5.2:25475220-25475239 None:intergenic 30.0%
TTTCTTCAGTTCTATTACCA+TGG + chr5.2:25474418-25474437 MS.gene43901:CDS 30.0%
! ATGTTGAACCAATGATTTTG+AGG - chr5.2:25474473-25474492 None:intergenic 30.0%
! CCTACAAACTGAAACATTTT+TGG + chr5.2:25475120-25475139 MS.gene43901:intron 30.0%
! GATATTTCTTGGGTGAATTT+TGG + chr5.2:25475445-25475464 MS.gene43901:intron 30.0%
! GCTTTTGTATACATGTTAAG+TGG - chr5.2:25475859-25475878 None:intergenic 30.0%
! TGTTGAACCAATGATTTTGA+GGG - chr5.2:25474472-25474491 None:intergenic 30.0%
! TTGCGACGAGAATATATTTT+CGG - chr5.2:25475614-25475633 None:intergenic 30.0%
!! TCATCAGTAGCAATTTTAGT+TGG + chr5.2:25475538-25475557 MS.gene43901:intron 30.0%
!!! CATCAGTAGCAATTTTAGTT+GGG + chr5.2:25475539-25475558 MS.gene43901:intron 30.0%
AAAGCTAGAACTACTAGATG+CGG + chr5.2:25475358-25475377 MS.gene43901:intron 35.0%
AACCAAAATTGAACCGGATT+TGG - chr5.2:25474579-25474598 None:intergenic 35.0%
AACTTCATAACGATGTTGAG+GGG - chr5.2:25474024-25474043 None:intergenic 35.0%
ACCAAGAATATGTATTCCCT+TGG - chr5.2:25474993-25475012 None:intergenic 35.0%
ACCAAGGGAATACATATTCT+TGG + chr5.2:25474989-25475008 MS.gene43901:intron 35.0%
ACTCTTCTGCTAGATTATGT+AGG + chr5.2:25475423-25475442 MS.gene43901:intron 35.0%
AGTTTACTTGCATTGCAGAA+TGG + chr5.2:25475822-25475841 MS.gene43901:CDS 35.0%
ATATTTAATTCCCTCCAGGA+GGG + chr5.2:25475735-25475754 MS.gene43901:intron 35.0%
ATTGGCTATATGCATATCCA+GGG + chr5.2:25475021-25475040 MS.gene43901:intron 35.0%
CAACTTCATAACGATGTTGA+GGG - chr5.2:25474025-25474044 None:intergenic 35.0%
GTGTTTGAAAATGTGAGCTA+TGG - chr5.2:25475642-25475661 None:intergenic 35.0%
TACTCGAACCAAAATTGAAC+CGG - chr5.2:25474585-25474604 None:intergenic 35.0%
TACTTCCAAAACACCAAATC+CGG + chr5.2:25474563-25474582 MS.gene43901:CDS 35.0%
TCTGATATTTAATTCCCTCC+AGG + chr5.2:25475731-25475750 MS.gene43901:intron 35.0%
! AGCTCACATTTTCAAACACA+TGG + chr5.2:25475643-25475662 MS.gene43901:intron 35.0%
! CCTTCTAAGAGATTTATCCA+CGG - chr5.2:25474879-25474898 None:intergenic 35.0%
! TTTTATAGAGGAGTTGCTGA+TGG + chr5.2:25474740-25474759 MS.gene43901:CDS 35.0%
!!! ATTTGGTGTTTTGGAAGTAG+AGG - chr5.2:25474562-25474581 None:intergenic 35.0%
!!! CTGTTTTTGAAGGCATAGTT+AGG - chr5.2:25475967-25475986 None:intergenic 35.0%
AACTATCCTTGGGAGTTTGT+TGG - chr5.2:25475209-25475228 None:intergenic 40.0%
AAGCTAGAACTACTAGATGC+GGG + chr5.2:25475359-25475378 MS.gene43901:intron 40.0%
AATTACATACCCCAGGATCA+AGG + chr5.2:25474320-25474339 MS.gene43901:CDS 40.0%
ATTACATACCCCAGGATCAA+GGG + chr5.2:25474321-25474340 MS.gene43901:CDS 40.0%
ATTCATGACCCATGAGAACA+AGG - chr5.2:25474303-25474322 None:intergenic 40.0%
ATTCTTTCACTCTCCTCCTT+AGG + chr5.2:25474446-25474465 MS.gene43901:CDS 40.0%
CAACATCGTTATGAAGTTGG+TGG + chr5.2:25474026-25474045 MS.gene43901:CDS 40.0%
CCAACTTCATAACGATGTTG+AGG - chr5.2:25474026-25474045 None:intergenic 40.0%
CCGTGGATAAATCTCTTAGA+AGG + chr5.2:25474876-25474895 MS.gene43901:CDS 40.0%
CCTCAACATCGTTATGAAGT+TGG + chr5.2:25474023-25474042 MS.gene43901:CDS 40.0%
CTGCGAGTAAACAGACTATT+GGG - chr5.2:25474628-25474647 None:intergenic 40.0%
CTTATTCCAACAAACTCCCA+AGG + chr5.2:25475200-25475219 MS.gene43901:intron 40.0%
GATATTTAATTCCCTCCAGG+AGG + chr5.2:25475734-25475753 MS.gene43901:intron 40.0%
GTTGGAAAAACAGCTTATGG+TGG + chr5.2:25474364-25474383 MS.gene43901:CDS 40.0%
TATTTAATTCCCTCCAGGAG+GGG + chr5.2:25475736-25475755 MS.gene43901:intron 40.0%
TCTGCGAGTAAACAGACTAT+TGG - chr5.2:25474629-25474648 None:intergenic 40.0%
TTGGCTATATGCATATCCAG+GGG + chr5.2:25475022-25475041 MS.gene43901:intron 40.0%
! AAAAACAGCTTATGGTGGAG+TGG + chr5.2:25474369-25474388 MS.gene43901:CDS 40.0%
! CAAGTGAGGAACTGATTTCT+CGG + chr5.2:25475782-25475801 MS.gene43901:CDS 40.0%
! CACCAAATCCGGTTCAATTT+TGG + chr5.2:25474574-25474593 MS.gene43901:CDS 40.0%
! CATTGGACGGCATCTTTTAA+AGG + chr5.2:25475759-25475778 MS.gene43901:CDS 40.0%
! CGAGTTGGAAAAACAGCTTA+TGG + chr5.2:25474361-25474380 MS.gene43901:CDS 40.0%
! GATTGGCTATATGCATATCC+AGG + chr5.2:25475020-25475039 MS.gene43901:intron 40.0%
! TGATGGTGAAGAAATGCAGT+TGG + chr5.2:25474757-25474776 MS.gene43901:CDS 40.0%
! TTTGAAGGCATAGTTAGGAG+AGG - chr5.2:25475962-25475981 None:intergenic 40.0%
!! CGAAGGATTGGGTTTTATAG+AGG + chr5.2:25474728-25474747 MS.gene43901:CDS 40.0%
!! GCATCTTTTAAAGGCAAGTG+AGG + chr5.2:25475768-25475787 MS.gene43901:CDS 40.0%
!!! CATGGCACATCTGTTTTTGA+AGG - chr5.2:25475977-25475996 None:intergenic 40.0%
!!! TTGAACCGGATTTGGTGTTT+TGG - chr5.2:25474571-25474590 None:intergenic 40.0%
AGCAAAGCCAATGACAATGG+TGG - chr5.2:25474234-25474253 None:intergenic 45.0%
ATAAGCGTAAGGCGAATCCT+TGG - chr5.2:25474849-25474868 None:intergenic 45.0%
CAAGTGCTTGCCGTAATTAC+CGG + chr5.2:25474092-25474111 MS.gene43901:CDS 45.0%
CCTTAGGCCCTCAAAATCAT+TGG + chr5.2:25474462-25474481 MS.gene43901:CDS 45.0%
GACAGCAAAGCCAATGACAA+TGG - chr5.2:25474237-25474256 None:intergenic 45.0%
GAGTGAAAGAATTGCTGCCA+TGG - chr5.2:25474438-25474457 None:intergenic 45.0%
GCAAGCACTTGAGAAGTTGA+TGG - chr5.2:25474083-25474102 None:intergenic 45.0%
GGCTGAAGAGGATGAAAAGT+GGG - chr5.2:25474196-25474215 None:intergenic 45.0%
GGTCATGAATTACATACCCC+AGG + chr5.2:25474313-25474332 MS.gene43901:CDS 45.0%
TAAGCGTAAGGCGAATCCTT+GGG - chr5.2:25474848-25474867 None:intergenic 45.0%
TACAGGAAGGCCGGTAATTA+CGG - chr5.2:25474105-25474124 None:intergenic 45.0%
TCCAGTGAGTGTTCCAAGAA+GGG - chr5.2:25474159-25474178 None:intergenic 45.0%
TTCGCCTTACGCTTATAGCT+TGG + chr5.2:25474853-25474872 MS.gene43901:CDS 45.0%
! TGGTTAGTTCGGAGTTTGTG+AGG - chr5.2:25474517-25474536 None:intergenic 45.0%
!! ATGATTTTGAGGGCCTAAGG+AGG - chr5.2:25474462-25474481 None:intergenic 45.0%
!! CCAATGATTTTGAGGGCCTA+AGG - chr5.2:25474465-25474484 None:intergenic 45.0%
!! CTTTGCTGTCGCTGTCTTTT+TGG + chr5.2:25474247-25474266 MS.gene43901:CDS 45.0%
!! GTGTTGCAGGTTGGTTAGTT+CGG - chr5.2:25474528-25474547 None:intergenic 45.0%
!!! GGTGTTTTGGAAGTAGAGGA+AGG - chr5.2:25474558-25474577 None:intergenic 45.0%
AAGCCAATGACAATGGTGGC+TGG - chr5.2:25474230-25474249 None:intergenic 50.0%
ACACTGGTATCGTAACAGCG+TGG - chr5.2:25473999-25474018 None:intergenic 50.0%
ACGATGTTGAGGGGTAACAC+TGG - chr5.2:25474015-25474034 None:intergenic 50.0%
ACGGCCAAGCTATAAGCGTA+AGG - chr5.2:25474860-25474879 None:intergenic 50.0%
ATGGTGGCTGGAACAAGAAC+AGG - chr5.2:25474218-25474237 None:intergenic 50.0%
CTTGGAACACTCACTGGACT+GGG + chr5.2:25474161-25474180 MS.gene43901:CDS 50.0%
GAAACTACACATTCAGCCCC+TGG - chr5.2:25475041-25475060 None:intergenic 50.0%
GAACAAGAACAGGGCTGAAG+AGG - chr5.2:25474208-25474227 None:intergenic 50.0%
GGAAGTAGAGGAAGGATTCG+AGG - chr5.2:25474550-25474569 None:intergenic 50.0%
GGCACTGTCCTTGTTCTCAT+GGG + chr5.2:25474292-25474311 MS.gene43901:CDS 50.0%
GGGCTGAAGAGGATGAAAAG+TGG - chr5.2:25474197-25474216 None:intergenic 50.0%
GGTGAAGAAATGCAGTTGGC+TGG + chr5.2:25474761-25474780 MS.gene43901:CDS 50.0%
GTAATTACCGGCCTTCCTGT+AGG + chr5.2:25474104-25474123 MS.gene43901:CDS 50.0%
GTCCAGTGAGTGTTCCAAGA+AGG - chr5.2:25474160-25474179 None:intergenic 50.0%
GTTCCAGCCACCATTGTCAT+TGG + chr5.2:25474224-25474243 MS.gene43901:CDS 50.0%
TCCCTTCTTGGAACACTCAC+TGG + chr5.2:25474155-25474174 MS.gene43901:CDS 50.0%
TCTACAGGAGCAACTCGAGT+TGG + chr5.2:25474346-25474365 MS.gene43901:CDS 50.0%
TCTTGGAACACTCACTGGAC+TGG + chr5.2:25474160-25474179 MS.gene43901:CDS 50.0%
TGGCACTGTCCTTGTTCTCA+TGG + chr5.2:25474291-25474310 MS.gene43901:CDS 50.0%
TGGTGGCTGGAACAAGAACA+GGG - chr5.2:25474217-25474236 None:intergenic 50.0%
TTACGCTTATAGCTTGGCCG+TGG + chr5.2:25474859-25474878 MS.gene43901:CDS 50.0%
TTCGTCTCTGCAACATCAGC+AGG - chr5.2:25475916-25475935 None:intergenic 50.0%
TTGGAACACTCACTGGACTG+GGG + chr5.2:25474162-25474181 MS.gene43901:CDS 50.0%
! AACAGCTTATGGTGGAGTGG+TGG + chr5.2:25474372-25474391 MS.gene43901:CDS 50.0%
! AACTCGAGTTGCTCCTGTAG+AGG - chr5.2:25474347-25474366 None:intergenic 50.0%
!! TGTCGCTGTCTTTTTGGCGT+CGG + chr5.2:25474253-25474272 MS.gene43901:CDS 50.0%
!!! TTTTGGCGTCGGCAGCTTTT+GGG + chr5.2:25474264-25474283 MS.gene43901:CDS 50.0%
!!! TTTTTGGCGTCGGCAGCTTT+TGG + chr5.2:25474263-25474282 MS.gene43901:CDS 50.0%
ATTACCGGCCTTCCTGTAGG+CGG + chr5.2:25474107-25474126 MS.gene43901:CDS 55.0%
CTGGTATCGTAACAGCGTGG+TGG - chr5.2:25473996-25474015 None:intergenic 55.0%
CTGTAGAGGCCCTTGATCCT+GGG - chr5.2:25474333-25474352 None:intergenic 55.0%
CTTGCTGGACTCTCCCTTCT+TGG + chr5.2:25474143-25474162 MS.gene43901:CDS 55.0%
GGTGTACTGCTTGCACTTGC+TGG + chr5.2:25474128-25474147 MS.gene43901:CDS 55.0%
GTTTGTGAGGACTCGACGAG+TGG - chr5.2:25474504-25474523 None:intergenic 55.0%
TGTAGAGGCCCTTGATCCTG+GGG - chr5.2:25474332-25474351 None:intergenic 55.0%
! GAGGACTCGACGAGTGGTTT+TGG - chr5.2:25474498-25474517 None:intergenic 55.0%
! GCTTATGGTGGAGTGGTGGT+CGG + chr5.2:25474376-25474395 MS.gene43901:CDS 55.0%
! TGGAACACTCACTGGACTGG+GGG + chr5.2:25474163-25474182 MS.gene43901:CDS 55.0%
!! GGAAGGATTCGAGGTGTTGC+AGG - chr5.2:25474541-25474560 None:intergenic 55.0%
!! GGATTCGAGGTGTTGCAGGT+TGG - chr5.2:25474537-25474556 None:intergenic 55.0%
AGGCGAATCCTTGGGAGGAG+TGG - chr5.2:25474840-25474859 None:intergenic 60.0%
CCAGGATCAAGGGCCTCTAC+AGG + chr5.2:25474331-25474350 MS.gene43901:CDS 60.0%
CCTGTAGAGGCCCTTGATCC+TGG - chr5.2:25474334-25474353 None:intergenic 60.0%
GCAAGCAGTACACCGCCTAC+AGG - chr5.2:25474122-25474141 None:intergenic 60.0%
GCAGTACACCGCCTACAGGA+AGG - chr5.2:25474118-25474137 None:intergenic 60.0%
GCGTAAGGCGAATCCTTGGG+AGG - chr5.2:25474845-25474864 None:intergenic 60.0%
GGCGAATCCTTGGGAGGAGT+GGG - chr5.2:25474839-25474858 None:intergenic 60.0%
TACACCGCCTACAGGAAGGC+CGG - chr5.2:25474114-25474133 None:intergenic 60.0%
TGCTTCACCCACTCCTCCCA+AGG + chr5.2:25474829-25474848 MS.gene43901:CDS 60.0%
! ATTCCCTCCAGGAGGGGCAT+TGG + chr5.2:25475742-25475761 MS.gene43901:intron 60.0%
! GTCGGCAGCTTTTGGGCTGA+TGG + chr5.2:25474271-25474290 MS.gene43901:CDS 60.0%
ATGCCGTCCAATGCCCCTCC+TGG - chr5.2:25475752-25475771 None:intergenic 65.0%
CGTCCAATGCCCCTCCTGGA+GGG - chr5.2:25475748-25475767 None:intergenic 65.0%
TGGTGGAGTGGTGGTCGGAG+TGG + chr5.2:25474381-25474400 MS.gene43901:CDS 65.0%
!! CCTCCAGGAGGGGCATTGGA+CGG + chr5.2:25475746-25475765 MS.gene43901:intron 65.0%
CCGTCCAATGCCCCTCCTGG+AGG - chr5.2:25475749-25475768 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 25473978 25476005 25473978 ID=MS.gene43901
chr5.2 mRNA 25473978 25476005 25473978 ID=MS.gene43901.t1;Parent=MS.gene43901
chr5.2 exon 25473978 25474897 25473978 ID=MS.gene43901.t1.exon1;Parent=MS.gene43901.t1
chr5.2 CDS 25473978 25474897 25473978 ID=cds.MS.gene43901.t1;Parent=MS.gene43901.t1
chr5.2 exon 25475753 25476005 25475753 ID=MS.gene43901.t1.exon2;Parent=MS.gene43901.t1
chr5.2 CDS 25475753 25476005 25475753 ID=cds.MS.gene43901.t1;Parent=MS.gene43901.t1
Gene Sequence

>MS.gene43901

ATGTCAGAACTTACACCACCACGCTGTTACGATACCAGTGTTACCCCTCAACATCGTTATGAAGTTGGTGGAGTTACTACTCATTACTCAGAGAAAAGAAGTCCATCAACTTCTCAAGTGCTTGCCGTAATTACCGGCCTTCCTGTAGGCGGTGTACTGCTTGCACTTGCTGGACTCTCCCTTCTTGGAACACTCACTGGACTGGGGGTAACAAGCCCACTTTTCATCCTCTTCAGCCCTGTTCTTGTTCCAGCCACCATTGTCATTGGCTTTGCTGTCGCTGTCTTTTTGGCGTCGGCAGCTTTTGGGCTGATGGCACTGTCCTTGTTCTCATGGGTCATGAATTACATACCCCAGGATCAAGGGCCTCTACAGGAGCAACTCGAGTTGGAAAAACAGCTTATGGTGGAGTGGTGGTCGGAGTGGTGTAACATGAAAGTTTTCTTCAGTTCTATTACCATGGCAGCAATTCTTTCACTCTCCTCCTTAGGCCCTCAAAATCATTGGTTCAACATCACCAAAACCACTCGTCGAGTCCTCACAAACTCCGAACTAACCAACCTGCAACACCTCGAATCCTTCCTCTACTTCCAAAACACCAAATCCGGTTCAATTTTGGTTCGAGTAATGAGAGACAACGAAATTGACCCAATAGTCTGTTTACTCGCAGATGCGTTCGCTGAATTGATGTTGTTCCCGAAATGTTATATCAACATGTTGAGATTCCTTATGAAACAATATTTGATTAAACGAAGGATTGGGTTTTATAGAGGAGTTGCTGATGGTGAAGAAATGCAGTTGGCTGGCACTGTTGAGATTTGTTTTGATAAAAATGGTGTTAATTCTTCTCGTGCTTCACCCACTCCTCCCAAGGATTCGCCTTACGCTTATAGCTTGGCCGTGGATAAATCTCTTAGAAGGTAAGCATTATTTATTGCGTCCAAGTTGCTGAAATTTGCATTTCTTTGGTAAAATTAGCTCAAGTGTCATGAACCATAAAGAACTAATTTGACCAAGGGAATACATATTCTTGGTCTAATTTGATTGGCTATATGCATATCCAGGGGCTGAATGTGTAGTTTCGATTATTCAAAGATTAAGGCTGTATTTAGATTGGTTTATGAATAACTTATTTGAGCTTACCTACAAACTGAAACATTTTTGGGTGTGTTTGTTAGAGCTTATGAAAATAGTTTAAAACATGTCCATAAGCTGATTTCAGCTTATTCCAACAAACTCCCAAGGATAGTTTTTGAACACAATTTGCAAATTATATGAAAACAGCTTAACCACATTTTCTTGTTGCTATTATAAGTAGCTTAGACATAAGCGCTAAATTAAGTTGTTTATTCAAACATACCCCAAGTGTATCTATATGTGAAAGCTAGAACTACTAGATGCGGGTTAAATCGATATTCAGAAACTAGTTTGATTATTAATTGTTAACTCTTCTGCTAGATTATGTAGGATATTTCTTGGGTGAATTTTGGAGACAGCTTGTCAGTATTATATACTTACAACAATTTTAACTTTTCTGATCTTACAGCTAGATAAATATTTTCATCAGTAGCAATTTTAGTTGGGAAAACCTGCAATTTAAATAATTAAGCTACCAAGTTTAAGATTCAAGTAACCGAAAATATATTCTCGTCGCAAAATTCCCATAGCTCACATTTTCAAACACATGGTATGTCTTCTCATTAAGCAAAAGAAAAAAAATCGATTGTCTGTTTTAACTTTCTAAAAACGTGTTTCTGATATTTAATTCCCTCCAGGAGGGGCATTGGACGGCATCTTTTAAAGGCAAGTGAGGAACTGATTTCTCGGATGAGTTCATCAAGAGAAGTTTACTTGCATTGCAGAATGGTTGATGAAGCTCCACTTAACATGTATACAAAAGCTGATTACAAAGTTGTTACACAGCAGTATTTTAGTCCTGCTGATGTTGCAGAGACGAAAACATTTGATGTGCAAGAAACTTCCTCTCCTAACTATGCCTTCAAAAACAGATGTGCCATGTTCCGATGA
Protein sequence

>MS.gene43901.t1

MSELTPPRCYDTSVTPQHRYEVGGVTTHYSEKRSPSTSQVLAVITGLPVGGVLLALAGLSLLGTLTGLGVTSPLFILFSPVLVPATIVIGFAVAVFLASAAFGLMALSLFSWVMNYIPQDQGPLQEQLELEKQLMVEWWSEWCNMKVFFSSITMAAILSLSSLGPQNHWFNITKTTRRVLTNSELTNLQHLESFLYFQNTKSGSILVRVMRDNEIDPIVCLLADAFAELMLFPKCYINMLRFLMKQYLIKRRIGFYRGVADGEEMQLAGTVEICFDKNGVNSSRASPTPPKDSPYAYSLAVDKSLRRRGIGRHLLKASEELISRMSSSREVYLHCRMVDEAPLNMYTKADYKVVTQQYFSPADVAETKTFDVQETSSPNYAFKNRCAMFR