AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44028

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44028.t1 MTR_1g081580 90.591 372 33 1 40 411 1 370 0.0 617
MS.gene44028.t1 MTR_7g114600 42.716 405 145 14 88 416 45 438 5.51e-86 270
MS.gene44028.t1 MTR_1g081560 37.113 291 137 9 127 412 123 372 2.57e-45 161
MS.gene44028.t1 MTR_1g081570 49.645 141 27 3 89 228 2 99 2.64e-31 115
MS.gene44028.t1 MTR_3g070120 37.037 135 75 3 136 270 153 277 1.09e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44028.t1 AT5G28040 42.021 376 170 14 82 417 60 427 2.04e-70 229
MS.gene44028.t1 AT5G28040 42.021 376 170 14 82 417 60 427 2.04e-70 229
MS.gene44028.t1 AT3G04930 40.749 427 176 18 59 412 29 451 1.28e-59 201
MS.gene44028.t1 AT3G04930 39.589 341 145 15 59 342 29 365 1.20e-41 152
MS.gene44028.t1 AT4G00390 32.271 251 140 7 24 272 51 273 2.14e-22 98.6
MS.gene44028.t1 AT1G61730 37.342 158 89 3 77 233 100 248 7.38e-22 97.1
MS.gene44028.t1 AT4G25210 42.991 107 56 2 131 233 136 241 1.99e-20 92.8
MS.gene44028.t1 AT4G01260 38.365 159 71 7 100 233 51 207 4.47e-18 85.1
MS.gene44028.t1 AT4G00610 32.394 142 87 2 95 236 108 240 6.43e-18 84.7
MS.gene44028.t1 AT1G44810 38.889 108 60 2 131 238 117 218 2.39e-16 79.7
MS.gene44028.t1 AT1G11510 33.962 159 78 2 136 294 109 240 2.00e-15 77.4
MS.gene44028.t1 AT1G11510 33.962 159 78 2 136 294 138 269 2.30e-15 77.4
MS.gene44028.t1 AT5G14280 36.957 92 58 0 138 229 63 154 4.31e-14 73.2
MS.gene44028.t1 AT4G00238 35.135 111 64 2 137 245 141 245 6.02e-14 73.2
MS.gene44028.t1 AT4G00250 36.111 108 63 1 137 244 116 217 9.10e-14 72.4
MS.gene44028.t1 AT2G25650 34.375 96 63 0 135 230 64 159 1.43e-13 72.4
MS.gene44028.t1 AT2G01370 32.743 113 71 3 129 239 72 181 2.30e-13 70.1
MS.gene44028.t1 AT4G00270 33.333 96 64 0 135 230 63 158 1.19e-12 68.9
MS.gene44028.t1 AT1G66420 33.051 118 65 3 136 246 87 197 1.41e-11 65.5

Find 137 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGTCAAATGAGAAGAATT+AGG 0.169998 1.3:-57570821 None:intergenic
CCTTACCAGAATCAAATTTC+TGG 0.175110 1.3:-57570661 None:intergenic
TAGGGTTAGGGTTGGGGTTT+GGG 0.179915 1.3:-57570802 None:intergenic
TTGAAATTTCACACAAAATC+TGG 0.187804 1.3:+57570720 MS.gene44028:CDS
TTAGGGTTAGGGTTGGGGTT+TGG 0.224438 1.3:-57570803 None:intergenic
TCTTCCATTGGCTCCCGTTT+TGG 0.265008 1.3:-57570413 None:intergenic
TTCATCCACATTGCTGCTAT+TGG 0.293962 1.3:-57570945 None:intergenic
ACCATGAGTGGGAGTCCTTT+TGG 0.299111 1.3:+57571073 MS.gene44028:CDS
AAGAATTAGGGTTAGGGTTG+GGG 0.305318 1.3:-57570808 None:intergenic
CTTATCAACAAGCTGGTTCT+TGG 0.314691 1.3:-57570603 None:intergenic
CAATGAATACTGTGCCATTT+GGG 0.343845 1.3:+57571131 MS.gene44028:CDS
CAGTCAAATGAGAAGAATTA+GGG 0.345673 1.3:-57570820 None:intergenic
GCTCTCCAGAAATTTGATTC+TGG 0.345969 1.3:+57570656 MS.gene44028:CDS
GAAGAATTAGGGTTAGGGTT+GGG 0.347770 1.3:-57570809 None:intergenic
TCAATGAATACTGTGCCATT+TGG 0.350621 1.3:+57571130 MS.gene44028:CDS
AAATGAGAAGAATTAGGGTT+AGG 0.361264 1.3:-57570815 None:intergenic
ATTGCTGCTATTGGTATTAC+CGG 0.364505 1.3:-57570936 None:intergenic
TCCTCCTATCATAACGATAC+TGG 0.368215 1.3:+57570536 MS.gene44028:CDS
GGAGGTGGGGACAGCGGCGG+TGG 0.386670 1.3:-57570330 None:intergenic
TGATAGTGGTGGTCGGTGTT+TGG 0.389979 1.3:-57570281 None:intergenic
GTGGCGGAAGCAGCAGATCT+TGG 0.391074 1.3:+57571195 MS.gene44028:CDS
ATATGATATTATCTATTGAT+TGG 0.394784 1.3:-57571347 None:intergenic
GATAATCATCAGAATAATTC+CGG 0.395019 1.3:+57570917 MS.gene44028:CDS
GCCGGAGCCGGAGCCGGAGC+TGG 0.407264 1.3:+57571307 MS.gene44028:CDS
TGAAATTTCACACAAAATCT+GGG 0.411047 1.3:+57570721 MS.gene44028:CDS
CGGCTCCCCTGAACCAAAAC+GGG 0.425461 1.3:+57570400 MS.gene44028:CDS
ACCAGCTTGTTGATAAGCTT+CGG 0.428179 1.3:+57570609 MS.gene44028:CDS
ATTTCCTCCTCTTCATCAAC+AGG 0.428859 1.3:-57570776 None:intergenic
TGTAATTGAGAAACCCTTGC+AGG 0.430967 1.3:-57570499 None:intergenic
TGGTGAGTGGTGAAGGACAC+GGG 0.433734 1.3:-57570122 None:intergenic
AGGAGGTGAAGGAGCGTGAC+CGG 0.436671 1.3:-57570145 None:intergenic
TCCAGCTCCGGCTCCGGCTC+CGG 0.439639 1.3:-57571308 None:intergenic
GGAGCCGGAGCTGGAGGTAG+TGG 0.440600 1.3:+57571316 MS.gene44028:CDS
AATGAGAAGAATTAGGGTTA+GGG 0.441702 1.3:-57570814 None:intergenic
GCTGGAGCTGGAGCCGGAGC+CGG 0.442271 1.3:+57571295 MS.gene44028:CDS
GGGGACAGCGGCGGTGGCGA+AGG 0.444218 1.3:-57570324 None:intergenic
CGATCCAGTATCGTTATGAT+AGG 0.445812 1.3:-57570540 None:intergenic
CCAGAAATTTGATTCTGGTA+AGG 0.445820 1.3:+57570661 MS.gene44028:CDS
TTTGTGTGAAATTTCAAAAG+TGG 0.448452 1.3:-57570714 None:intergenic
GAAGGTTGACGGAGATTGTT+CGG 0.453008 1.3:-57570306 None:intergenic
GCTGGAGCCGGAGCCGGAGC+CGG 0.457654 1.3:+57571301 MS.gene44028:CDS
CTGTTTGACACCGGTGTTGA+AGG 0.458464 1.3:+57571036 MS.gene44028:CDS
CTGATGCGGAGGCGTGTGGT+AGG 0.462324 1.3:-57570364 None:intergenic
TGCCATGAGAGAGGAGGTGA+AGG 0.463374 1.3:-57570156 None:intergenic
AAGAGAAGACCAGCGATTCT+CGG 0.466132 1.3:+57570432 MS.gene44028:CDS
AACGGTAATTCAACGCCAAT+TGG 0.467910 1.3:+57570749 MS.gene44028:CDS
TCGCTGGTCTTCTCTTCCAT+TGG 0.470929 1.3:-57570425 None:intergenic
TCATCAACAGGTACACCAAT+TGG 0.471428 1.3:-57570764 None:intergenic
GAGTAACTCTTCAGTGTTGG+TGG 0.471480 1.3:-57570078 None:intergenic
GCGGCGGTGGCGAAGGTTGA+CGG 0.475689 1.3:-57570317 None:intergenic
TGGTTCAGGGGAGCCGTCGA+TGG 0.476050 1.3:-57570393 None:intergenic
CGATGTCGGCGTAATCATCA+TGG 0.477676 1.3:-57570208 None:intergenic
GAGATTGTTCGGTTTGATAG+TGG 0.479733 1.3:-57570295 None:intergenic
TTGAGATCGAATGCTGGAGC+TGG 0.483868 1.3:+57571283 MS.gene44028:CDS
TCGAATGCTGGAGCTGGAGC+CGG 0.484287 1.3:+57571289 MS.gene44028:CDS
ATTTCCACTACCTCCAGCTC+CGG 0.484287 1.3:-57571320 None:intergenic
TAGGGCTAAGATGACGGAGG+TGG 0.485162 1.3:-57570345 None:intergenic
CTCCTTCACCTCCTCTCTCA+TGG 0.487848 1.3:+57570154 MS.gene44028:CDS
ACTACCTCCAGCTCCGGCTC+CGG 0.492756 1.3:-57571314 None:intergenic
CGGTGTCAAACAGCTCTTCA+CGG 0.494859 1.3:-57571026 None:intergenic
GAAGCAGCAGATCTTGGAGC+TGG 0.497446 1.3:+57571201 MS.gene44028:CDS
CGCAATCTCGCAAGAGATCG+CGG 0.499446 1.3:+57570855 MS.gene44028:CDS
CACAAAATCTGGGGCAGCAA+CGG 0.505174 1.3:+57570731 MS.gene44028:CDS
TTTGAGTAACTCTTCAGTGT+TGG 0.509237 1.3:-57570081 None:intergenic
GAGATCGCGGGCAGCTGAGG+AGG 0.510050 1.3:+57570868 MS.gene44028:CDS
ACGGCTCCCCTGAACCAAAA+CGG 0.512535 1.3:+57570399 MS.gene44028:CDS
GAAGCGGTTGGAGCTGGTGC+AGG 0.515736 1.3:+57571234 MS.gene44028:CDS
GTGGTGAGTGGTGAAGGACA+CGG 0.516740 1.3:-57570123 None:intergenic
ACGGTCTCTTCGATTAATCC+AGG 0.519165 1.3:-57571007 None:intergenic
CTCATGGCATTCTCTCGAAA+CGG 0.520566 1.3:+57570170 MS.gene44028:CDS
AGGGCTAAGATGACGGAGGT+GGG 0.524316 1.3:-57570344 None:intergenic
CAAGAGATCGCGGGCAGCTG+AGG 0.524761 1.3:+57570865 MS.gene44028:CDS
GTATTCGAAGCGGTTGGAGC+TGG 0.525755 1.3:+57571228 MS.gene44028:CDS
AGTGGTGGTCGGTGTTTGGT+TGG 0.541859 1.3:-57570277 None:intergenic
ATCGTTCTATGATCACATGA+AGG 0.546638 1.3:+57570559 MS.gene44028:CDS
TTCGGTTTGATAGTGGTGGT+CGG 0.548380 1.3:-57570288 None:intergenic
GCAATCTCGCAAGAGATCGC+GGG 0.549804 1.3:+57570856 MS.gene44028:CDS
TGAGATCAAGGTTGCGATCG+AGG 0.551018 1.3:+57571258 MS.gene44028:CDS
TGAACCAAAACGGGAGCCAA+TGG 0.551623 1.3:+57570409 MS.gene44028:CDS
CATGAAGGGGAAGATTCAGC+TGG 0.556910 1.3:+57570574 MS.gene44028:CDS
TCCGAAGCTTATCAACAAGC+TGG 0.557121 1.3:-57570610 None:intergenic
CGACGACGATTTAGAAGAAG+AGG 0.558405 1.3:+57570241 MS.gene44028:CDS
GATGACGGAGGTGGGGACAG+CGG 0.558674 1.3:-57570336 None:intergenic
GACGAAATCACGATCCTGCA+AGG 0.561286 1.3:+57570485 MS.gene44028:CDS
TCCAGTATCGTTATGATAGG+AGG 0.562775 1.3:-57570537 None:intergenic
AGAAGAATTAGGGTTAGGGT+TGG 0.562837 1.3:-57570810 None:intergenic
TGACCGGTGGTGAGTGGTGA+AGG 0.563496 1.3:-57570129 None:intergenic
GAGGAGTTGAGATCGAATGC+TGG 0.564654 1.3:+57571277 MS.gene44028:CDS
CAAATTTCTGGAGAGCAGTA+CGG 0.564956 1.3:-57570649 None:intergenic
GTCGTCTTCTCCCTCGATGT+CGG 0.573199 1.3:-57570222 None:intergenic
AATCATGCACATAATGTACC+TGG 0.574034 1.3:+57570989 MS.gene44028:CDS
GATGATTACGCCGACATCGA+GGG 0.574634 1.3:+57570212 MS.gene44028:CDS
ATTGTTCGGTTTGATAGTGG+TGG 0.575227 1.3:-57570292 None:intergenic
TCGGCGTAATCATCATGGGT+GGG 0.575332 1.3:-57570203 None:intergenic
TCGTTCTATGATCACATGAA+GGG 0.576436 1.3:+57570560 MS.gene44028:CDS
TTCGATAACTCCTTCAACAC+CGG 0.578505 1.3:-57571046 None:intergenic
GGAGCCGGAGCCGGAGCTGG+AGG 0.581056 1.3:+57571310 MS.gene44028:CDS
GTGTGGTAGGGCTAAGATGA+CGG 0.582706 1.3:-57570351 None:intergenic
CAGTGTTGGTGGCAATCAGA+AGG 0.582904 1.3:-57570067 None:intergenic
GGAGGTGTATTCGAAGCGGT+TGG 0.582984 1.3:+57571222 MS.gene44028:CDS
GAAAATCGTGATAGAAGTAA+CGG 0.584019 1.3:+57570965 MS.gene44028:CDS
TGATGCGGAGGCGTGTGGTA+GGG 0.584219 1.3:-57570363 None:intergenic
TGTCCTTCACCACTCACCAC+CGG 0.584407 1.3:+57570126 MS.gene44028:CDS
GTCGATGGTTGTTGCTGATG+CGG 0.592509 1.3:-57570378 None:intergenic
CGGCGTAATCATCATGGGTG+GGG 0.593339 1.3:-57570202 None:intergenic
AAAAGGCGCTGGATGAAAAG+TGG 0.594253 1.3:+57571176 MS.gene44028:CDS
GCTGGTGCAGGATGAGATCA+AGG 0.595852 1.3:+57571246 MS.gene44028:CDS
TCGAGAGAATGCCATGAGAG+AGG 0.597757 1.3:-57570165 None:intergenic
AGGTGAAGGAGCGTGACCGG+TGG 0.597834 1.3:-57570142 None:intergenic
ACCAAAAGGACTCCCACTCA+TGG 0.598783 1.3:-57571074 None:intergenic
TTATCGAATGCTACCATGAG+TGG 0.599446 1.3:+57571061 MS.gene44028:CDS
GTCGGCGTAATCATCATGGG+TGG 0.603570 1.3:-57570204 None:intergenic
TTTCGTCTTCTTCAGTCCAG+AGG 0.609633 1.3:-57570469 None:intergenic
GGAGCGTGACCGGTGGTGAG+TGG 0.612696 1.3:-57570135 None:intergenic
GGGCTAAGATGACGGAGGTG+GGG 0.612836 1.3:-57570343 None:intergenic
GAAATTTCACACAAAATCTG+GGG 0.613296 1.3:+57570722 MS.gene44028:CDS
GATGTCGGCGTAATCATCAT+GGG 0.614670 1.3:-57570207 None:intergenic
CGTTCTATGATCACATGAAG+GGG 0.621138 1.3:+57570561 MS.gene44028:CDS
GACGGAGGTGGGGACAGCGG+CGG 0.622679 1.3:-57570333 None:intergenic
AGCTGGAGGTGTATTCGAAG+CGG 0.623559 1.3:+57571218 MS.gene44028:CDS
TGGTGTACCTGTTGATGAAG+AGG 0.630690 1.3:+57570769 MS.gene44028:CDS
GTTGCTGATGCGGAGGCGTG+TGG 0.634510 1.3:-57570368 None:intergenic
CGTGAAGAGCTGTTTGACAC+CGG 0.636688 1.3:+57571027 MS.gene44028:CDS
ACGAAATCACGATCCTGCAA+GGG 0.639447 1.3:+57570486 MS.gene44028:CDS
AGAGAATGCCATGAGAGAGG+AGG 0.642601 1.3:-57570162 None:intergenic
TGATGATTACGCCGACATCG+AGG 0.642948 1.3:+57570211 MS.gene44028:CDS
AGCTTGTTGATAAGCTTCGG+AGG 0.643387 1.3:+57570612 MS.gene44028:CDS
TATCGAATGCTACCATGAGT+GGG 0.643483 1.3:+57571062 MS.gene44028:CDS
GGCATTCTCTCGAAACGGCG+TGG 0.645669 1.3:+57570175 MS.gene44028:CDS
GCAGCAGATCTTGGAGCTGG+AGG 0.646814 1.3:+57571204 MS.gene44028:CDS
GGTGAGTGGTGAAGGACACG+GGG 0.670289 1.3:-57570121 None:intergenic
GCATCAGCAACAACCATCGA+CGG 0.685479 1.3:+57570380 MS.gene44028:CDS
AGGCGCTGGATGAAAAGTGG+CGG 0.689339 1.3:+57571179 MS.gene44028:CDS
TAATACCAATAGCAGCAATG+TGG 0.690177 1.3:+57570940 MS.gene44028:CDS
GATGGTTGTTGCTGATGCGG+AGG 0.694249 1.3:-57570375 None:intergenic
TGTACCTGTTGATGAAGAGG+AGG 0.712098 1.3:+57570772 MS.gene44028:CDS
TGGTAGGGCTAAGATGACGG+AGG 0.739720 1.3:-57570348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATAATCATCAGAATAATTC+CGG + chr1.3:57570917-57570936 MS.gene44028:CDS 25.0%
! TGAAATTTCACACAAAATCT+GGG + chr1.3:57570721-57570740 MS.gene44028:CDS 25.0%
! TTGAAATTTCACACAAAATC+TGG + chr1.3:57570720-57570739 MS.gene44028:CDS 25.0%
! TTTGTGTGAAATTTCAAAAG+TGG - chr1.3:57570717-57570736 None:intergenic 25.0%
AAATGAGAAGAATTAGGGTT+AGG - chr1.3:57570818-57570837 None:intergenic 30.0%
AATGAGAAGAATTAGGGTTA+GGG - chr1.3:57570817-57570836 None:intergenic 30.0%
CAGTCAAATGAGAAGAATTA+GGG - chr1.3:57570823-57570842 None:intergenic 30.0%
GAAAATCGTGATAGAAGTAA+CGG + chr1.3:57570965-57570984 MS.gene44028:CDS 30.0%
GAAATTTCACACAAAATCTG+GGG + chr1.3:57570722-57570741 MS.gene44028:CDS 30.0%
TCAGTCAAATGAGAAGAATT+AGG - chr1.3:57570824-57570843 None:intergenic 30.0%
!! ATTTGGGTTTTTGAATTTGG+GGG + chr1.3:57571147-57571166 MS.gene44028:CDS 30.0%
!! CATTTGGGTTTTTGAATTTG+GGG + chr1.3:57571146-57571165 MS.gene44028:CDS 30.0%
!! CCATTTGGGTTTTTGAATTT+GGG + chr1.3:57571145-57571164 MS.gene44028:CDS 30.0%
AATCATGCACATAATGTACC+TGG + chr1.3:57570989-57571008 MS.gene44028:CDS 35.0%
ATCGTTCTATGATCACATGA+AGG + chr1.3:57570559-57570578 MS.gene44028:CDS 35.0%
ATTGCTGCTATTGGTATTAC+CGG - chr1.3:57570939-57570958 None:intergenic 35.0%
CCAGAAATTTGATTCTGGTA+AGG + chr1.3:57570661-57570680 MS.gene44028:CDS 35.0%
CCCAAATTCAAAAACCCAAA+TGG - chr1.3:57571148-57571167 None:intergenic 35.0%
CCTTACCAGAATCAAATTTC+TGG - chr1.3:57570664-57570683 None:intergenic 35.0%
TAATACCAATAGCAGCAATG+TGG + chr1.3:57570940-57570959 MS.gene44028:CDS 35.0%
TCGTTCTATGATCACATGAA+GGG + chr1.3:57570560-57570579 MS.gene44028:CDS 35.0%
TTTGAGTAACTCTTCAGTGT+TGG - chr1.3:57570084-57570103 None:intergenic 35.0%
! TCAATGAATACTGTGCCATT+TGG + chr1.3:57571130-57571149 MS.gene44028:CDS 35.0%
!! CAATGAATACTGTGCCATTT+GGG + chr1.3:57571131-57571150 MS.gene44028:CDS 35.0%
!! GCCATTTGGGTTTTTGAATT+TGG + chr1.3:57571144-57571163 MS.gene44028:CDS 35.0%
!! TTTGGGTTTTTGAATTTGGG+GGG + chr1.3:57571148-57571167 MS.gene44028:CDS 35.0%
AACGGTAATTCAACGCCAAT+TGG + chr1.3:57570749-57570768 MS.gene44028:CDS 40.0%
AAGAATTAGGGTTAGGGTTG+GGG - chr1.3:57570811-57570830 None:intergenic 40.0%
AGAAGAATTAGGGTTAGGGT+TGG - chr1.3:57570813-57570832 None:intergenic 40.0%
ATTTCCTCCTCTTCATCAAC+AGG - chr1.3:57570779-57570798 None:intergenic 40.0%
CGATCCAGTATCGTTATGAT+AGG - chr1.3:57570543-57570562 None:intergenic 40.0%
CGTTCTATGATCACATGAAG+GGG + chr1.3:57570561-57570580 MS.gene44028:CDS 40.0%
GAAGAATTAGGGTTAGGGTT+GGG - chr1.3:57570812-57570831 None:intergenic 40.0%
GCTCTCCAGAAATTTGATTC+TGG + chr1.3:57570656-57570675 MS.gene44028:CDS 40.0%
TATCGAATGCTACCATGAGT+GGG + chr1.3:57571062-57571081 MS.gene44028:CDS 40.0%
TCATCAACAGGTACACCAAT+TGG - chr1.3:57570767-57570786 None:intergenic 40.0%
TCCAGTATCGTTATGATAGG+AGG - chr1.3:57570540-57570559 None:intergenic 40.0%
TCCTCCTATCATAACGATAC+TGG + chr1.3:57570536-57570555 MS.gene44028:CDS 40.0%
TGTAATTGAGAAACCCTTGC+AGG - chr1.3:57570502-57570521 None:intergenic 40.0%
TTATCGAATGCTACCATGAG+TGG + chr1.3:57571061-57571080 MS.gene44028:CDS 40.0%
TTCATCCACATTGCTGCTAT+TGG - chr1.3:57570948-57570967 None:intergenic 40.0%
TTCGATAACTCCTTCAACAC+CGG - chr1.3:57571049-57571068 None:intergenic 40.0%
! CAAATTTCTGGAGAGCAGTA+CGG - chr1.3:57570652-57570671 None:intergenic 40.0%
! CTTATCAACAAGCTGGTTCT+TGG - chr1.3:57570606-57570625 None:intergenic 40.0%
! GAGATTGTTCGGTTTGATAG+TGG - chr1.3:57570298-57570317 None:intergenic 40.0%
!! ACCAGCTTGTTGATAAGCTT+CGG + chr1.3:57570609-57570628 MS.gene44028:CDS 40.0%
!! ATTGTTCGGTTTGATAGTGG+TGG - chr1.3:57570295-57570314 None:intergenic 40.0%
AAAAAGCTTCCGAGAATCGC+TGG - chr1.3:57570444-57570463 None:intergenic 45.0%
AAGAGAAGACCAGCGATTCT+CGG + chr1.3:57570432-57570451 MS.gene44028:CDS 45.0%
ACGAAATCACGATCCTGCAA+GGG + chr1.3:57570486-57570505 MS.gene44028:CDS 45.0%
ACGGTCTCTTCGATTAATCC+AGG - chr1.3:57571010-57571029 None:intergenic 45.0%
CGACGACGATTTAGAAGAAG+AGG + chr1.3:57570241-57570260 MS.gene44028:CDS 45.0%
CTCATGGCATTCTCTCGAAA+CGG + chr1.3:57570170-57570189 MS.gene44028:CDS 45.0%
GAAGGTTGACGGAGATTGTT+CGG - chr1.3:57570309-57570328 None:intergenic 45.0%
GATGTCGGCGTAATCATCAT+GGG - chr1.3:57570210-57570229 None:intergenic 45.0%
TCCGAAGCTTATCAACAAGC+TGG - chr1.3:57570613-57570632 None:intergenic 45.0%
TTTCGTCTTCTTCAGTCCAG+AGG - chr1.3:57570472-57570491 None:intergenic 45.0%
! GAGTAACTCTTCAGTGTTGG+TGG - chr1.3:57570081-57570100 None:intergenic 45.0%
! TGGTGTACCTGTTGATGAAG+AGG + chr1.3:57570769-57570788 MS.gene44028:CDS 45.0%
! TGTACCTGTTGATGAAGAGG+AGG + chr1.3:57570772-57570791 MS.gene44028:CDS 45.0%
!! AAAAGGCGCTGGATGAAAAG+TGG + chr1.3:57571176-57571195 MS.gene44028:CDS 45.0%
!! AGCTTGTTGATAAGCTTCGG+AGG + chr1.3:57570612-57570631 MS.gene44028:CDS 45.0%
!! TTCGGTTTGATAGTGGTGGT+CGG - chr1.3:57570291-57570310 None:intergenic 45.0%
ACCAAAAGGACTCCCACTCA+TGG - chr1.3:57571077-57571096 None:intergenic 50.0%
AGAGAATGCCATGAGAGAGG+AGG - chr1.3:57570165-57570184 None:intergenic 50.0%
ATTTCCACTACCTCCAGCTC+CGG - chr1.3:57571323-57571342 None:intergenic 50.0%
CATGAAGGGGAAGATTCAGC+TGG + chr1.3:57570574-57570593 MS.gene44028:CDS 50.0%
CGATGTCGGCGTAATCATCA+TGG - chr1.3:57570211-57570230 None:intergenic 50.0%
CGTGAAGAGCTGTTTGACAC+CGG + chr1.3:57571027-57571046 MS.gene44028:CDS 50.0%
GACGAAATCACGATCCTGCA+AGG + chr1.3:57570485-57570504 MS.gene44028:CDS 50.0%
GAGGAGTTGAGATCGAATGC+TGG + chr1.3:57571277-57571296 MS.gene44028:CDS 50.0%
GATGATTACGCCGACATCGA+GGG + chr1.3:57570212-57570231 MS.gene44028:CDS 50.0%
GCATCAGCAACAACCATCGA+CGG + chr1.3:57570380-57570399 MS.gene44028:CDS 50.0%
GTCGATGGTTGTTGCTGATG+CGG - chr1.3:57570381-57570400 None:intergenic 50.0%
GTGTGGTAGGGCTAAGATGA+CGG - chr1.3:57570354-57570373 None:intergenic 50.0%
TAGGGTTAGGGTTGGGGTTT+GGG - chr1.3:57570805-57570824 None:intergenic 50.0%
TCGAGAGAATGCCATGAGAG+AGG - chr1.3:57570168-57570187 None:intergenic 50.0%
TCGGCGTAATCATCATGGGT+GGG - chr1.3:57570206-57570225 None:intergenic 50.0%
TGAACCAAAACGGGAGCCAA+TGG + chr1.3:57570409-57570428 MS.gene44028:CDS 50.0%
TGAGATCAAGGTTGCGATCG+AGG + chr1.3:57571258-57571277 MS.gene44028:CDS 50.0%
TGATGATTACGCCGACATCG+AGG + chr1.3:57570211-57570230 MS.gene44028:CDS 50.0%
TTAGGGTTAGGGTTGGGGTT+TGG - chr1.3:57570806-57570825 None:intergenic 50.0%
TTGAGATCGAATGCTGGAGC+TGG + chr1.3:57571283-57571302 MS.gene44028:CDS 50.0%
! ACCATGAGTGGGAGTCCTTT+TGG + chr1.3:57571073-57571092 MS.gene44028:CDS 50.0%
! AGCTGGAGGTGTATTCGAAG+CGG + chr1.3:57571218-57571237 MS.gene44028:CDS 50.0%
! AGTGGGAGTCCTTTTGGTCT+TGG + chr1.3:57571079-57571098 MS.gene44028:CDS 50.0%
! CACAAAATCTGGGGCAGCAA+CGG + chr1.3:57570731-57570750 MS.gene44028:CDS 50.0%
! CGGTGTCAAACAGCTCTTCA+CGG - chr1.3:57571029-57571048 None:intergenic 50.0%
! CTGTTTGACACCGGTGTTGA+AGG + chr1.3:57571036-57571055 MS.gene44028:CDS 50.0%
! GGGTTTTTGAATTTGGGGGG+CGG + chr1.3:57571151-57571170 MS.gene44028:CDS 50.0%
!! CAGTGTTGGTGGCAATCAGA+AGG - chr1.3:57570070-57570089 None:intergenic 50.0%
!! GTGGGAGTCCTTTTGGTCTT+GGG + chr1.3:57571080-57571099 MS.gene44028:CDS 50.0%
!! TCGCTGGTCTTCTCTTCCAT+TGG - chr1.3:57570428-57570447 None:intergenic 50.0%
!! TGATAGTGGTGGTCGGTGTT+TGG - chr1.3:57570284-57570303 None:intergenic 50.0%
!!! ATTGGCTCCCGTTTTGGTTC+AGG - chr1.3:57570410-57570429 None:intergenic 50.0%
!!! CTTTTGGTCTTGGGGGAAGA+GGG + chr1.3:57571089-57571108 MS.gene44028:CDS 50.0%
!!! TCTTCCATTGGCTCCCGTTT+TGG - chr1.3:57570416-57570435 None:intergenic 50.0%
!!! TGGGAGTCCTTTTGGTCTTG+GGG + chr1.3:57571081-57571100 MS.gene44028:CDS 50.0%
!!! TTGGCTCCCGTTTTGGTTCA+GGG - chr1.3:57570409-57570428 None:intergenic 50.0%
ACGGCTCCCCTGAACCAAAA+CGG + chr1.3:57570399-57570418 MS.gene44028:CDS 55.0%
CCTCTTCCCCCAAGACCAAA+AGG - chr1.3:57571091-57571110 None:intergenic 55.0%
CGCAATCTCGCAAGAGATCG+CGG + chr1.3:57570855-57570874 MS.gene44028:CDS 55.0%
CGGCGTAATCATCATGGGTG+GGG - chr1.3:57570205-57570224 None:intergenic 55.0%
CTCCTTCACCTCCTCTCTCA+TGG + chr1.3:57570154-57570173 MS.gene44028:CDS 55.0%
GAAGCAGCAGATCTTGGAGC+TGG + chr1.3:57571201-57571220 MS.gene44028:CDS 55.0%
GAATTTGGGGGGCGGAGAAA+AGG + chr1.3:57571159-57571178 MS.gene44028:CDS 55.0%
GATGGTTGTTGCTGATGCGG+AGG - chr1.3:57570378-57570397 None:intergenic 55.0%
GCAATCTCGCAAGAGATCGC+GGG + chr1.3:57570856-57570875 MS.gene44028:CDS 55.0%
GCTGGTGCAGGATGAGATCA+AGG + chr1.3:57571246-57571265 MS.gene44028:CDS 55.0%
GTATTCGAAGCGGTTGGAGC+TGG + chr1.3:57571228-57571247 MS.gene44028:CDS 55.0%
GTCGGCGTAATCATCATGGG+TGG - chr1.3:57570207-57570226 None:intergenic 55.0%
GTCGTCTTCTCCCTCGATGT+CGG - chr1.3:57570225-57570244 None:intergenic 55.0%
GTGGTGAGTGGTGAAGGACA+CGG - chr1.3:57570126-57570145 None:intergenic 55.0%
TGCCATGAGAGAGGAGGTGA+AGG - chr1.3:57570159-57570178 None:intergenic 55.0%
TGGTGAGTGGTGAAGGACAC+GGG - chr1.3:57570125-57570144 None:intergenic 55.0%
TGTCCTTCACCACTCACCAC+CGG + chr1.3:57570126-57570145 MS.gene44028:CDS 55.0%
! AGTGGTGGTCGGTGTTTGGT+TGG - chr1.3:57570280-57570299 None:intergenic 55.0%
! GGAGGTGTATTCGAAGCGGT+TGG + chr1.3:57571222-57571241 MS.gene44028:CDS 55.0%
! TGGTAGGGCTAAGATGACGG+AGG - chr1.3:57570351-57570370 None:intergenic 55.0%
!! AGGCGCTGGATGAAAAGTGG+CGG + chr1.3:57571179-57571198 MS.gene44028:CDS 55.0%
!! AGGGCTAAGATGACGGAGGT+GGG - chr1.3:57570347-57570366 None:intergenic 55.0%
!! TAGGGCTAAGATGACGGAGG+TGG - chr1.3:57570348-57570367 None:intergenic 55.0%
!!! CCTTTTGGTCTTGGGGGAAG+AGG + chr1.3:57571088-57571107 MS.gene44028:CDS 55.0%
!!! GGAAGCTTTTTCAGCGCCTC+TGG + chr1.3:57570453-57570472 MS.gene44028:CDS 55.0%
!!! GGGAGTCCTTTTGGTCTTGG+GGG + chr1.3:57571082-57571101 MS.gene44028:CDS 55.0%
!!! TGGCTCCCGTTTTGGTTCAG+GGG - chr1.3:57570408-57570427 None:intergenic 55.0%
CGGCTCCCCTGAACCAAAAC+GGG + chr1.3:57570400-57570419 MS.gene44028:CDS 60.0%
GCAGCAGATCTTGGAGCTGG+AGG + chr1.3:57571204-57571223 MS.gene44028:CDS 60.0%
GGCATTCTCTCGAAACGGCG+TGG + chr1.3:57570175-57570194 MS.gene44028:CDS 60.0%
GGTCTTGGGGGAAGAGGGTT+TGG + chr1.3:57571094-57571113 MS.gene44028:CDS 60.0%
GGTGAGTGGTGAAGGACACG+GGG - chr1.3:57570124-57570143 None:intergenic 60.0%
TCGAATGCTGGAGCTGGAGC+CGG + chr1.3:57571289-57571308 MS.gene44028:CDS 60.0%
TGACCGGTGGTGAGTGGTGA+AGG - chr1.3:57570132-57570151 None:intergenic 60.0%
! AGGAGGTGAAGGAGCGTGAC+CGG - chr1.3:57570148-57570167 None:intergenic 60.0%
! GTGGCGGAAGCAGCAGATCT+TGG + chr1.3:57571195-57571214 MS.gene44028:CDS 60.0%
! TGATGCGGAGGCGTGTGGTA+GGG - chr1.3:57570366-57570385 None:intergenic 60.0%
!! GGGCTAAGATGACGGAGGTG+GGG - chr1.3:57570346-57570365 None:intergenic 60.0%
ACTACCTCCAGCTCCGGCTC+CGG - chr1.3:57571317-57571336 None:intergenic 65.0%
CAAGAGATCGCGGGCAGCTG+AGG + chr1.3:57570865-57570884 MS.gene44028:CDS 65.0%
GAAGCGGTTGGAGCTGGTGC+AGG + chr1.3:57571234-57571253 MS.gene44028:CDS 65.0%
TGGTTCAGGGGAGCCGTCGA+TGG - chr1.3:57570396-57570415 None:intergenic 65.0%
! AGGTGAAGGAGCGTGACCGG+TGG - chr1.3:57570145-57570164 None:intergenic 65.0%
! CTGATGCGGAGGCGTGTGGT+AGG - chr1.3:57570367-57570386 None:intergenic 65.0%
! GGGGGAAGAGGGTTTGGAGC+TGG + chr1.3:57571100-57571119 MS.gene44028:CDS 65.0%
! GTTGCTGATGCGGAGGCGTG+TGG - chr1.3:57570371-57570390 None:intergenic 65.0%
!! GATGACGGAGGTGGGGACAG+CGG - chr1.3:57570339-57570358 None:intergenic 65.0%
GAGATCGCGGGCAGCTGAGG+AGG + chr1.3:57570868-57570887 MS.gene44028:CDS 70.0%
GCGGCGGTGGCGAAGGTTGA+CGG - chr1.3:57570320-57570339 None:intergenic 70.0%
GGAGCCGGAGCTGGAGGTAG+TGG + chr1.3:57571316-57571335 MS.gene44028:CDS 70.0%
GGAGCGTGACCGGTGGTGAG+TGG - chr1.3:57570138-57570157 None:intergenic 70.0%
GACGGAGGTGGGGACAGCGG+CGG - chr1.3:57570336-57570355 None:intergenic 75.0%
GCTGGAGCTGGAGCCGGAGC+CGG + chr1.3:57571295-57571314 MS.gene44028:CDS 75.0%
TCCAGCTCCGGCTCCGGCTC+CGG - chr1.3:57571311-57571330 None:intergenic 75.0%
! GGGGGGCGGAGAAAAGGCGC+TGG + chr1.3:57571165-57571184 MS.gene44028:CDS 75.0%
! GCTGGAGCCGGAGCCGGAGC+CGG + chr1.3:57571301-57571320 MS.gene44028:CDS 80.0%
! GGAGCCGGAGCCGGAGCTGG+AGG + chr1.3:57571310-57571329 MS.gene44028:CDS 80.0%
! GGAGGTGGGGACAGCGGCGG+TGG - chr1.3:57570333-57570352 None:intergenic 80.0%
! GGGGACAGCGGCGGTGGCGA+AGG - chr1.3:57570327-57570346 None:intergenic 80.0%
! GCCGGAGCCGGAGCCGGAGC+TGG + chr1.3:57571307-57571326 MS.gene44028:CDS 85.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 57570056 57571357 57570056 ID=MS.gene44028
chr1.3 mRNA 57570056 57571357 57570056 ID=MS.gene44028.t1;Parent=MS.gene44028
chr1.3 exon 57570056 57571357 57570056 ID=MS.gene44028.t1.exon1;Parent=MS.gene44028.t1
chr1.3 CDS 57570056 57571357 57570056 ID=cds.MS.gene44028.t1;Parent=MS.gene44028.t1
Gene Sequence

>MS.gene44028

ATGCATTTTCTCCTTCTGATTGCCACCAACACTGAAGAGTTACTCAAACAACACAACATAACAAACCCCGTGTCCTTCACCACTCACCACCGGTCACGCTCCTTCACCTCCTCTCTCATGGCATTCTCTCGAAACGGCGTGGTTATCCCCACCCATGATGATTACGCCGACATCGAGGGAGAAGACGACGACGATTTAGAAGAAGAGGACAACTACTTTGTCCAACCAAACACCGACCACCACTATCAAACCGAACAATCTCCGTCAACCTTCGCCACCGCCGCTGTCCCCACCTCCGTCATCTTAGCCCTACCACACGCCTCCGCATCAGCAACAACCATCGACGGCTCCCCTGAACCAAAACGGGAGCCAATGGAAGAGAAGACCAGCGATTCTCGGAAGCTTTTTCAGCGCCTCTGGACTGAAGAAGACGAAATCACGATCCTGCAAGGGTTTCTCAATTACAACGCTAATCGTGAATCCTCCTATCATAACGATACTGGATCGTTCTATGATCACATGAAGGGGAAGATTCAGCTGGATTTCACCAAGAACCAGCTTGTTGATAAGCTTCGGAGGCTGAAGAAGAAGTACCGTACTGCTCTCCAGAAATTTGATTCTGGTAAGGATTTTACTTTCAAAAGTGCTCATGATCAAGCCACTTTTGAAATTTCACACAAAATCTGGGGCAGCAACGGTAATTCAACGCCAATTGGTGTACCTGTTGATGAAGAGGAGGAAATGAGCCCAAACCCCAACCCTAACCCTAATTCTTCTCATTTGACTGAGAAGAAAGCTGCGCAATCTCGCAAGAGATCGCGGGCAGCTGAGGAGGAAAAGCGTGAGTTGACTGATTCTAGAGATAATCATCAGAATAATTCCGGTAATACCAATAGCAGCAATGTGGATGAAAATCGTGATAGAAGTAACGGTAATCATGCACATAATGTACCTGGATTAATCGAAGAGACCGTGAAGAGCTGTTTGACACCGGTGTTGAAGGAGTTATCGAATGCTACCATGAGTGGGAGTCCTTTTGGTCTTGGGGGAAGAGGGTTTGGAGCTGGAGCTTTTTCAATGAATACTGTGCCATTTGGGTTTTTGAATTTGGGGGGCGGAGAAAAGGCGCTGGATGAAAAGTGGCGGAAGCAGCAGATCTTGGAGCTGGAGGTGTATTCGAAGCGGTTGGAGCTGGTGCAGGATGAGATCAAGGTTGCGATCGAGGAGTTGAGATCGAATGCTGGAGCTGGAGCCGGAGCCGGAGCCGGAGCTGGAGGTAGTGGAAATAATACCAATCAATAG
Protein sequence

>MS.gene44028.t1

MHFLLLIATNTEELLKQHNITNPVSFTTHHRSRSFTSSLMAFSRNGVVIPTHDDYADIEGEDDDDLEEEDNYFVQPNTDHHYQTEQSPSTFATAAVPTSVILALPHASASATTIDGSPEPKREPMEEKTSDSRKLFQRLWTEEDEITILQGFLNYNANRESSYHNDTGSFYDHMKGKIQLDFTKNQLVDKLRRLKKKYRTALQKFDSGKDFTFKSAHDQATFEISHKIWGSNGNSTPIGVPVDEEEEMSPNPNPNPNSSHLTEKKAAQSRKRSRAAEEEKRELTDSRDNHQNNSGNTNSSNVDENRDRSNGNHAHNVPGLIEETVKSCLTPVLKELSNATMSGSPFGLGGRGFGAGAFSMNTVPFGFLNLGGGEKALDEKWRKQQILELEVYSKRLELVQDEIKVAIEELRSNAGAGAGAGAGAGGSGNNTNQ