AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 92 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGAACGTGGCTAATTTC+TGG 0.218063 1.3:+59247973 MS.gene44157:CDS
AGAGCATAGAGTTTGGTTTC+AGG 0.263168 1.3:+59247389 MS.gene44157:CDS
GGCGGTGGTTGCTTCTATTC+AGG 0.264711 1.3:+59247456 MS.gene44157:CDS
TGTTCTTCCACCACTGGTAC+TGG 0.276664 1.3:-59247918 None:intergenic
AATTAGCCACGTTCTGAATC+TGG 0.296437 1.3:-59247968 None:intergenic
GAGGAACCTGCTTTCGATAT+TGG 0.318864 1.3:+59248005 MS.gene44157:CDS
GAGTTGTGCGCATCATTAAT+TGG 0.323647 1.3:-59247852 None:intergenic
AAATTTGCTCGGCGAGCATT+TGG 0.329497 1.3:+59248286 MS.gene44157:CDS
GTCAAATAGAGCATAGAGTT+TGG 0.371033 1.3:+59247382 MS.gene44157:CDS
TTAAAACATGTGTCTCCAAC+TGG 0.380379 1.3:-59248097 None:intergenic
CGATGTTGAATACCCTCCTT+TGG 0.387542 1.3:-59246683 None:intergenic
GATCATTATGAGCAACCATT+AGG 0.389483 1.3:+59247495 MS.gene44157:CDS
CATTCCTGATGATGGTGATA+TGG 0.395267 1.3:+59247881 MS.gene44157:CDS
GCACGTGAGGCAGGTCGTTA+TGG 0.400336 1.3:+59248507 MS.gene44157:CDS
TCAGAACGTGGCTAATTTCT+GGG 0.416922 1.3:+59247974 MS.gene44157:CDS
GCTACCGCAGCGGCAGTTTG+TGG 0.431281 1.3:-59247945 None:intergenic
AATCGTAGGTTTAGACCTAA+TGG 0.433227 1.3:-59247510 None:intergenic
AAACATCCCGTTATCGTCAT+AGG 0.435778 1.3:-59247535 None:intergenic
AATTTGCTCGGCGAGCATTT+GGG 0.441935 1.3:+59248287 MS.gene44157:CDS
CATCATTAATTGGATTTGAC+GGG 0.443530 1.3:-59247842 None:intergenic
GCCTCACGTGCAGGACAGTT+GGG 0.444919 1.3:-59248495 None:intergenic
TCATTCCACTTAATATCGAT+TGG 0.456651 1.3:-59248361 None:intergenic
CGTTAGAGAGAACAGATTTG+TGG 0.458615 1.3:+59248435 MS.gene44157:CDS
TCCACCACTGGTACTGGGTC+AGG 0.460719 1.3:-59247912 None:intergenic
GTTCCACCGGGTTTCGAAGT+AGG 0.463343 1.3:+59247816 MS.gene44157:CDS
GATACCAGAGGGTCTAGGTT+TGG 0.463429 1.3:+59247411 MS.gene44157:CDS
GTTCTTCCACCACTGGTACT+GGG 0.464509 1.3:-59247917 None:intergenic
GATTGACCTATGACGATAAC+GGG 0.465542 1.3:+59247529 MS.gene44157:CDS
GCATCATTAATTGGATTTGA+CGG 0.475292 1.3:-59247843 None:intergenic
TGCCTCACGTGCAGGACAGT+TGG 0.476556 1.3:-59248496 None:intergenic
TTTAACGCTTTCTGCGTACA+TGG 0.478595 1.3:+59248115 MS.gene44157:CDS
AGTATCCAATCGATATTAAG+TGG 0.481943 1.3:+59248356 MS.gene44157:CDS
TCATCCATGTCAAGAGTGAA+TGG 0.486104 1.3:-59247567 None:intergenic
CTCATCTCGGATCGTGATGA+AGG 0.492063 1.3:-59248323 None:intergenic
TCTCCTCCCATTCCTGATGA+TGG 0.497542 1.3:+59247873 MS.gene44157:CDS
TCTAGGTTTGGAGTATGTTA+CGG 0.497585 1.3:+59247423 MS.gene44157:CDS
CGATTGACCTATGACGATAA+CGG 0.500068 1.3:+59247528 MS.gene44157:CDS
AACTGTCCTGCACGTGAGGC+AGG 0.503776 1.3:+59248498 MS.gene44157:CDS
TTTATTTCCAGCAGTTCCAC+CGG 0.508663 1.3:+59247803 MS.gene44157:intron
TTCAGGATACCAGAGGGTCT+AGG 0.513988 1.3:+59247406 MS.gene44157:CDS
TACTCCAAACCTAGACCCTC+TGG 0.515790 1.3:-59247415 None:intergenic
ATTAGCCACGTTCTGAATCT+GGG 0.515846 1.3:-59247967 None:intergenic
CATATCACCATCATCAGGAA+TGG 0.516230 1.3:-59247880 None:intergenic
TGATAATGAGGAGGCTGCTA+TGG 0.520057 1.3:+59248028 MS.gene44157:CDS
TTTCGATATTGGTGATAATG+AGG 0.526872 1.3:+59248016 MS.gene44157:CDS
AGCTTACGGCTCGTGTCCCA+TGG 0.534486 1.3:+59247337 MS.gene44157:intron
TAACGACCTGCCTCACGTGC+AGG 0.537930 1.3:-59248504 None:intergenic
ACTACCATATCACCATCATC+AGG 0.542080 1.3:-59247885 None:intergenic
TCTCGGATCGTGATGAAGGT+TGG 0.548455 1.3:-59248319 None:intergenic
TAAAACATGTGTCTCCAACT+GGG 0.552768 1.3:-59248096 None:intergenic
ATATCACCATCATCAGGAAT+GGG 0.554219 1.3:-59247879 None:intergenic
TTGTACCAGCAAAATTTGCT+CGG 0.557897 1.3:+59248275 MS.gene44157:CDS
ATTCACTCTTGACATGGATG+AGG 0.568212 1.3:+59247569 MS.gene44157:CDS
GCTTCCATTCACTCTTGACA+TGG 0.570881 1.3:+59247563 MS.gene44157:CDS
GTATGTTACGGAGAGATCAA+TGG 0.580224 1.3:+59247435 MS.gene44157:CDS
ACAGATTTGTGGAAGGCACA+AGG 0.581028 1.3:+59248446 MS.gene44157:CDS
TGACATGCAACCTTGAGCTA+TGG 0.582304 1.3:-59247620 None:intergenic
GTTGCTCATAATGATCAAGC+AGG 0.585077 1.3:-59247488 None:intergenic
AATACCGATGTAGCTTTATG+CGG 0.587466 1.3:-59248388 None:intergenic
TTATCACCAATATCGAAAGC+AGG 0.592587 1.3:-59248011 None:intergenic
TTATTTCCAGCAGTTCCACC+GGG 0.594229 1.3:+59247804 MS.gene44157:intron
ACCCAACTGTCCTGCACGTG+AGG 0.595873 1.3:+59248494 MS.gene44157:CDS
CCACCGGGTTTCGAAGTAGG+AGG 0.598715 1.3:+59247819 MS.gene44157:CDS
TGCACATGGCAATCCCCAGT+TGG 0.598828 1.3:+59248082 MS.gene44157:CDS
AAAACATGTGTCTCCAACTG+GGG 0.599659 1.3:-59248095 None:intergenic
AGGTAACTAATCGTCAATTG+AGG 0.606077 1.3:+59247589 MS.gene44157:CDS
CGATATTGGTGATAATGAGG+AGG 0.613156 1.3:+59248019 MS.gene44157:CDS
TGTTCCTGACCCAGTACCAG+TGG 0.613436 1.3:+59247908 MS.gene44157:CDS
TACGGAGAGATCAATGGCGG+TGG 0.619550 1.3:+59247441 MS.gene44157:CDS
CAGATTTGTGGAAGGCACAA+GGG 0.620094 1.3:+59248447 MS.gene44157:CDS
TATCGTCATAGGTCAATCGT+AGG 0.623984 1.3:-59247524 None:intergenic
ACTCACGGTGGAACTCATCT+CGG 0.624872 1.3:-59248336 None:intergenic
TCCTGACCCAGTACCAGTGG+TGG 0.629390 1.3:+59247911 MS.gene44157:CDS
TAATATCGATTGGATACTCA+CGG 0.630661 1.3:-59248351 None:intergenic
AGAGAGAACAGATTTGTGGA+AGG 0.635968 1.3:+59248439 MS.gene44157:CDS
GTTTGGTTTCAGGATACCAG+AGG 0.643279 1.3:+59247399 MS.gene44157:CDS
TTTGGTTTCAGGATACCAGA+GGG 0.645318 1.3:+59247400 MS.gene44157:CDS
TCACCATCATCAGGAATGGG+AGG 0.646579 1.3:-59247876 None:intergenic
TAATTTCTGGGATGATGATG+AGG 0.648095 1.3:+59247986 MS.gene44157:CDS
GGAACTATGACCATAGCTCA+AGG 0.651950 1.3:+59247610 MS.gene44157:CDS
AAATCCACAAACTGCCGCTG+CGG 0.654912 1.3:+59247941 MS.gene44157:CDS
AAAACCGCATAAAGCTACAT+CGG 0.665649 1.3:+59248384 MS.gene44157:CDS
CTGAATCTGGGCTACCGCAG+CGG 0.666979 1.3:-59247955 None:intergenic
GGGCCTCCTACTTCGAAACC+CGG 0.671468 1.3:-59247822 None:intergenic
AATAGATGTGGTAGATGCTG+AGG 0.672789 1.3:+59248058 MS.gene44157:CDS
CATGGTGAAATGCAGCAACA+TGG 0.687154 1.3:+59248133 MS.gene44157:CDS
CCTCCTACTTCGAAACCCGG+TGG 0.702465 1.3:-59247819 None:intergenic
GGTAGCCCAGATTCAGAACG+TGG 0.706210 1.3:+59247962 MS.gene44157:CDS
TATGGATGTCGAAATAGATG+TGG 0.725685 1.3:+59248046 MS.gene44157:CDS
TGTTACGGAGAGATCAATGG+CGG 0.735510 1.3:+59247438 MS.gene44157:CDS
GTAGATGCTGAGGATGCACA+TGG 0.739903 1.3:+59248068 MS.gene44157:CDS
TATCGATTGGATACTCACGG+TGG 0.816467 1.3:-59248348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAGAAGAAAAAAAAAA+CGG - chr1.3:59246904-59246923 None:intergenic 10.0%
!!! ATGGTTTTTTTTTTTAAATA+AGG - chr1.3:59247076-59247095 None:intergenic 10.0%
!!! TGGTTTTTTTTTTTAAATAA+GGG - chr1.3:59247075-59247094 None:intergenic 10.0%
!!! TTATAGATTTTCAATACTTT+TGG + chr1.3:59247273-59247292 MS.gene44157:intron 15.0%
!! CTTTATACAGTTATTTATTG+TGG + chr1.3:59248195-59248214 MS.gene44157:intron 20.0%
!! TATTGAAAATCTATAAGCAA+AGG - chr1.3:59247269-59247288 None:intergenic 20.0%
! ACACAACAAAATACAAAGAA+TGG - chr1.3:59247120-59247139 None:intergenic 25.0%
! ATAAATAACTGTATAAAGCG+TGG - chr1.3:59248193-59248212 None:intergenic 25.0%
! GTGTATGAGAAACAAAATTA+TGG - chr1.3:59247095-59247114 None:intergenic 25.0%
! TACAGTTATTTATTGTGGTT+TGG + chr1.3:59248200-59248219 MS.gene44157:intron 25.0%
!!! TATTTTTACGAACGTTTCAA+AGG + chr1.3:59247360-59247379 MS.gene44157:CDS 25.0%
AAACGTTCGTAAAAATACCA+TGG - chr1.3:59247357-59247376 None:intergenic 30.0%
AACGTTCGTAAAAATACCAT+GGG - chr1.3:59247356-59247375 None:intergenic 30.0%
AGTATCCAATCGATATTAAG+TGG + chr1.3:59248356-59248375 MS.gene44157:CDS 30.0%
CATCATTAATTGGATTTGAC+GGG - chr1.3:59247845-59247864 None:intergenic 30.0%
CCTTCAAAAAAGAGAAAAAC+TGG - chr1.3:59247168-59247187 None:intergenic 30.0%
GCATCATTAATTGGATTTGA+CGG - chr1.3:59247846-59247865 None:intergenic 30.0%
GTTGTGTTGTGTCAAATAAA+TGG + chr1.3:59247133-59247152 MS.gene44157:intron 30.0%
TCATTCCACTTAATATCGAT+TGG - chr1.3:59248364-59248383 None:intergenic 30.0%
TGTTTGTATTCTGTAGCTTA+CGG + chr1.3:59247323-59247342 MS.gene44157:intron 30.0%
! CAGCAGCATTAATTTGAAAT+TGG - chr1.3:59247725-59247744 None:intergenic 30.0%
! TAATATCGATTGGATACTCA+CGG - chr1.3:59248354-59248373 None:intergenic 30.0%
! TTTCGATATTGGTGATAATG+AGG + chr1.3:59248016-59248035 MS.gene44157:CDS 30.0%
!!! CCAGTTTTTCTCTTTTTTGA+AGG + chr1.3:59247165-59247184 MS.gene44157:intron 30.0%
AAAACCGCATAAAGCTACAT+CGG + chr1.3:59248384-59248403 MS.gene44157:CDS 35.0%
AAAATCTATAAGCAAAGGCG+AGG - chr1.3:59247264-59247283 None:intergenic 35.0%
AAATAAATCAGCAGCAGCAA+CGG - chr1.3:59247790-59247809 None:intergenic 35.0%
AAATCTATAAGCAAAGGCGA+GGG - chr1.3:59247263-59247282 None:intergenic 35.0%
AATCGTAGGTTTAGACCTAA+TGG - chr1.3:59247513-59247532 None:intergenic 35.0%
AATTAATGCTGCTGCTATTG+TGG + chr1.3:59247731-59247750 MS.gene44157:intron 35.0%
AGGTAACTAATCGTCAATTG+AGG + chr1.3:59247589-59247608 MS.gene44157:CDS 35.0%
ATATCACCATCATCAGGAAT+GGG - chr1.3:59247882-59247901 None:intergenic 35.0%
ATTAATGCTGCTGCTATTGT+GGG + chr1.3:59247732-59247751 MS.gene44157:intron 35.0%
GATCATTATGAGCAACCATT+AGG + chr1.3:59247495-59247514 MS.gene44157:CDS 35.0%
GTCAAATAGAGCATAGAGTT+TGG + chr1.3:59247382-59247401 MS.gene44157:CDS 35.0%
TAAAACATGTGTCTCCAACT+GGG - chr1.3:59248099-59248118 None:intergenic 35.0%
TATGGATGTCGAAATAGATG+TGG + chr1.3:59248046-59248065 MS.gene44157:CDS 35.0%
TTAAAACATGTGTCTCCAAC+TGG - chr1.3:59248100-59248119 None:intergenic 35.0%
TTATCACCAATATCGAAAGC+AGG - chr1.3:59248014-59248033 None:intergenic 35.0%
! AAGCTACATCGGTATTTTTC+AGG + chr1.3:59248395-59248414 MS.gene44157:CDS 35.0%
! AGCATCGCATTTTGAATCAT+TGG - chr1.3:59246750-59246769 None:intergenic 35.0%
! AGCTACATCGGTATTTTTCA+GGG + chr1.3:59248396-59248415 MS.gene44157:CDS 35.0%
! CAAAGTATCTAAATGTACGC+AGG - chr1.3:59247653-59247672 None:intergenic 35.0%
! TCTAGGTTTGGAGTATGTTA+CGG + chr1.3:59247423-59247442 MS.gene44157:CDS 35.0%
! TTGTACCAGCAAAATTTGCT+CGG + chr1.3:59248275-59248294 MS.gene44157:CDS 35.0%
!! AATACCGATGTAGCTTTATG+CGG - chr1.3:59248391-59248410 None:intergenic 35.0%
!! TAATTTCTGGGATGATGATG+AGG + chr1.3:59247986-59248005 MS.gene44157:CDS 35.0%
AAAACATGTGTCTCCAACTG+GGG - chr1.3:59248098-59248117 None:intergenic 40.0%
AAACATCCCGTTATCGTCAT+AGG - chr1.3:59247538-59247557 None:intergenic 40.0%
AATAGATGTGGTAGATGCTG+AGG + chr1.3:59248058-59248077 MS.gene44157:CDS 40.0%
AATGCAACATCCACCAGATA+TGG - chr1.3:59246819-59246838 None:intergenic 40.0%
ACTACCATATCACCATCATC+AGG - chr1.3:59247888-59247907 None:intergenic 40.0%
AGAGAGAACAGATTTGTGGA+AGG + chr1.3:59248439-59248458 MS.gene44157:CDS 40.0%
ATTCACTCTTGACATGGATG+AGG + chr1.3:59247569-59247588 MS.gene44157:CDS 40.0%
CATATCACCATCATCAGGAA+TGG - chr1.3:59247883-59247902 None:intergenic 40.0%
CGATTGACCTATGACGATAA+CGG + chr1.3:59247528-59247547 MS.gene44157:CDS 40.0%
CGTTAGAGAGAACAGATTTG+TGG + chr1.3:59248435-59248454 MS.gene44157:CDS 40.0%
GAGTTGTGCGCATCATTAAT+TGG - chr1.3:59247855-59247874 None:intergenic 40.0%
GATTGACCTATGACGATAAC+GGG + chr1.3:59247529-59247548 MS.gene44157:CDS 40.0%
GTATGTTACGGAGAGATCAA+TGG + chr1.3:59247435-59247454 MS.gene44157:CDS 40.0%
GTTGCTCATAATGATCAAGC+AGG - chr1.3:59247491-59247510 None:intergenic 40.0%
TATCGTCATAGGTCAATCGT+AGG - chr1.3:59247527-59247546 None:intergenic 40.0%
TCAGAACGTGGCTAATTTCT+GGG + chr1.3:59247974-59247993 MS.gene44157:CDS 40.0%
TCATCCATGTCAAGAGTGAA+TGG - chr1.3:59247570-59247589 None:intergenic 40.0%
TTCAGAACGTGGCTAATTTC+TGG + chr1.3:59247973-59247992 MS.gene44157:CDS 40.0%
TTTAACGCTTTCTGCGTACA+TGG + chr1.3:59248115-59248134 MS.gene44157:CDS 40.0%
TTTATTTCCAGCAGTTCCAC+CGG + chr1.3:59247803-59247822 MS.gene44157:intron 40.0%
! AATTAGCCACGTTCTGAATC+TGG - chr1.3:59247971-59247990 None:intergenic 40.0%
! ACCAGATATGGAAGTGTATC+GGG - chr1.3:59246807-59246826 None:intergenic 40.0%
! AGAGCATAGAGTTTGGTTTC+AGG + chr1.3:59247389-59247408 MS.gene44157:CDS 40.0%
! ATTAGCCACGTTCTGAATCT+GGG - chr1.3:59247970-59247989 None:intergenic 40.0%
! CACCAGATATGGAAGTGTAT+CGG - chr1.3:59246808-59246827 None:intergenic 40.0%
! CGATATTGGTGATAATGAGG+AGG + chr1.3:59248019-59248038 MS.gene44157:CDS 40.0%
! TAGAGCACCATACCTAGTTT+GGG - chr1.3:59246716-59246735 None:intergenic 40.0%
! TTAGAGCACCATACCTAGTT+TGG - chr1.3:59246717-59246736 None:intergenic 40.0%
! TTTGGTTTCAGGATACCAGA+GGG + chr1.3:59247400-59247419 MS.gene44157:CDS 40.0%
!! CATTCCTGATGATGGTGATA+TGG + chr1.3:59247881-59247900 MS.gene44157:CDS 40.0%
!! GCTACATCGGTATTTTTCAG+GGG + chr1.3:59248397-59248416 MS.gene44157:CDS 40.0%
!! TGCATGCATTGATTCATCAG+TGG + chr1.3:59246838-59246857 MS.gene44157:intron 40.0%
AGACGAAGACGAAGATGAGT+CGG - chr1.3:59247679-59247698 None:intergenic 45.0%
CATGGTGAAATGCAGCAACA+TGG + chr1.3:59248133-59248152 MS.gene44157:CDS 45.0%
CGATACACTTCCATATCTGG+TGG + chr1.3:59246806-59246825 MS.gene44157:intron 45.0%
CGATGTTGAATACCCTCCTT+TGG - chr1.3:59246686-59246705 None:intergenic 45.0%
GAGGAACCTGCTTTCGATAT+TGG + chr1.3:59248005-59248024 MS.gene44157:CDS 45.0%
GCTTCCATTCACTCTTGACA+TGG + chr1.3:59247563-59247582 MS.gene44157:CDS 45.0%
GGAACTATGACCATAGCTCA+AGG + chr1.3:59247610-59247629 MS.gene44157:CDS 45.0%
TCCCGATACACTTCCATATC+TGG + chr1.3:59246803-59246822 MS.gene44157:intron 45.0%
TCGTCTTCGTCATCTGATGA+TGG + chr1.3:59247699-59247718 MS.gene44157:intron 45.0%
TGACATGCAACCTTGAGCTA+TGG - chr1.3:59247623-59247642 None:intergenic 45.0%
TGTTACGGAGAGATCAATGG+CGG + chr1.3:59247438-59247457 MS.gene44157:CDS 45.0%
TTATTTCCAGCAGTTCCACC+GGG + chr1.3:59247804-59247823 MS.gene44157:intron 45.0%
! AAATTTGCTCGGCGAGCATT+TGG + chr1.3:59248286-59248305 MS.gene44157:CDS 45.0%
! AATTTGCTCGGCGAGCATTT+GGG + chr1.3:59248287-59248306 MS.gene44157:CDS 45.0%
! ACAGATTTGTGGAAGGCACA+AGG + chr1.3:59248446-59248465 MS.gene44157:CDS 45.0%
! CAGATTTGTGGAAGGCACAA+GGG + chr1.3:59248447-59248466 MS.gene44157:CDS 45.0%
! GGATTTTGTTCTTCCACCAC+TGG - chr1.3:59247927-59247946 None:intergenic 45.0%
! GTTTGGTTTCAGGATACCAG+AGG + chr1.3:59247399-59247418 MS.gene44157:CDS 45.0%
! TATCGATTGGATACTCACGG+TGG - chr1.3:59248351-59248370 None:intergenic 45.0%
! TGATAATGAGGAGGCTGCTA+TGG + chr1.3:59248028-59248047 MS.gene44157:CDS 45.0%
!! AAGGAGGGTATTCAACATCG+CGG + chr1.3:59246686-59246705 MS.gene44157:intron 45.0%
!! GTTGCTGTTGGTGGTGATAA+TGG + chr1.3:59247762-59247781 MS.gene44157:intron 45.0%
!!! ACATCGGTATTTTTCAGGGG+CGG + chr1.3:59248400-59248419 MS.gene44157:CDS 45.0%
AAATCCACAAACTGCCGCTG+CGG + chr1.3:59247941-59247960 MS.gene44157:CDS 50.0%
ACTCACGGTGGAACTCATCT+CGG - chr1.3:59248339-59248358 None:intergenic 50.0%
CGGTCAACCCAAACTAGGTA+TGG + chr1.3:59246706-59246725 MS.gene44157:intron 50.0%
CTCATCTCGGATCGTGATGA+AGG - chr1.3:59248326-59248345 None:intergenic 50.0%
GATACCAGAGGGTCTAGGTT+TGG + chr1.3:59247411-59247430 MS.gene44157:CDS 50.0%
GTAGATGCTGAGGATGCACA+TGG + chr1.3:59248068-59248087 MS.gene44157:CDS 50.0%
GTTCTTCCACCACTGGTACT+GGG - chr1.3:59247920-59247939 None:intergenic 50.0%
TACTCCAAACCTAGACCCTC+TGG - chr1.3:59247418-59247437 None:intergenic 50.0%
TCACCATCATCAGGAATGGG+AGG - chr1.3:59247879-59247898 None:intergenic 50.0%
TCTCCTCCCATTCCTGATGA+TGG + chr1.3:59247873-59247892 MS.gene44157:CDS 50.0%
TCTCGGATCGTGATGAAGGT+TGG - chr1.3:59248322-59248341 None:intergenic 50.0%
TGTTCTTCCACCACTGGTAC+TGG - chr1.3:59247921-59247940 None:intergenic 50.0%
TTCAGGATACCAGAGGGTCT+AGG + chr1.3:59247406-59247425 MS.gene44157:CDS 50.0%
!!! GGTATTTTTCAGGGGCGGTT+GGG + chr1.3:59248405-59248424 MS.gene44157:CDS 50.0%
!!! GTATTTTTCAGGGGCGGTTG+GGG + chr1.3:59248406-59248425 MS.gene44157:CDS 50.0%
CATCGCGGTCAACCCAAACT+AGG + chr1.3:59246701-59246720 MS.gene44157:intron 55.0%
GCTGCTGCTATTGTGGGTTC+TGG + chr1.3:59247738-59247757 MS.gene44157:intron 55.0%
GGCGGTGGTTGCTTCTATTC+AGG + chr1.3:59247456-59247475 MS.gene44157:CDS 55.0%
GGTAGCCCAGATTCAGAACG+TGG + chr1.3:59247962-59247981 MS.gene44157:CDS 55.0%
GTTCCACCGGGTTTCGAAGT+AGG + chr1.3:59247816-59247835 MS.gene44157:CDS 55.0%
TACGGAGAGATCAATGGCGG+TGG + chr1.3:59247441-59247460 MS.gene44157:CDS 55.0%
TGCACATGGCAATCCCCAGT+TGG + chr1.3:59248082-59248101 MS.gene44157:CDS 55.0%
TGTTCCTGACCCAGTACCAG+TGG + chr1.3:59247908-59247927 MS.gene44157:CDS 55.0%
! GCTCGCCGAGCAAATTTTGC+TGG - chr1.3:59248283-59248302 None:intergenic 55.0%
!! GGTTCTGGTGTTGCTGTTGG+TGG + chr1.3:59247753-59247772 MS.gene44157:intron 55.0%
!! GTGGGTTCTGGTGTTGCTGT+TGG + chr1.3:59247750-59247769 MS.gene44157:intron 55.0%
!!! CGGTATTTTTCAGGGGCGGT+TGG + chr1.3:59248404-59248423 MS.gene44157:CDS 55.0%
AACTGTCCTGCACGTGAGGC+AGG + chr1.3:59248498-59248517 MS.gene44157:CDS 60.0%
ACCCAACTGTCCTGCACGTG+AGG + chr1.3:59248494-59248513 MS.gene44157:CDS 60.0%
AGCTTACGGCTCGTGTCCCA+TGG + chr1.3:59247337-59247356 MS.gene44157:intron 60.0%
CCACCGGGTTTCGAAGTAGG+AGG + chr1.3:59247819-59247838 MS.gene44157:CDS 60.0%
CCTCCTACTTCGAAACCCGG+TGG - chr1.3:59247822-59247841 None:intergenic 60.0%
CTGAATCTGGGCTACCGCAG+CGG - chr1.3:59247958-59247977 None:intergenic 60.0%
GCACGTGAGGCAGGTCGTTA+TGG + chr1.3:59248507-59248526 MS.gene44157:CDS 60.0%
GCCTCACGTGCAGGACAGTT+GGG - chr1.3:59248498-59248517 None:intergenic 60.0%
GGGCCTCCTACTTCGAAACC+CGG - chr1.3:59247825-59247844 None:intergenic 60.0%
TAACGACCTGCCTCACGTGC+AGG - chr1.3:59248507-59248526 None:intergenic 60.0%
TCCTGACCCAGTACCAGTGG+TGG + chr1.3:59247911-59247930 MS.gene44157:CDS 60.0%
TCGAAACCCGGTGGAACTGC+TGG - chr1.3:59247813-59247832 None:intergenic 60.0%
TGCCTCACGTGCAGGACAGT+TGG - chr1.3:59248499-59248518 None:intergenic 60.0%
! TCCACCACTGGTACTGGGTC+AGG - chr1.3:59247915-59247934 None:intergenic 60.0%
GCTACCGCAGCGGCAGTTTG+TGG - chr1.3:59247948-59247967 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59246675 59248534 59246675 ID=MS.gene44157
chr1.3 mRNA 59246675 59248534 59246675 ID=MS.gene44157.t1;Parent=MS.gene44157
chr1.3 exon 59246675 59246692 59246675 ID=MS.gene44157.t1.exon1;Parent=MS.gene44157.t1
chr1.3 CDS 59246675 59246692 59246675 ID=cds.MS.gene44157.t1;Parent=MS.gene44157.t1
chr1.3 exon 59247339 59247654 59247339 ID=MS.gene44157.t1.exon2;Parent=MS.gene44157.t1
chr1.3 CDS 59247339 59247654 59247339 ID=cds.MS.gene44157.t1;Parent=MS.gene44157.t1
chr1.3 exon 59247814 59248154 59247814 ID=MS.gene44157.t1.exon3;Parent=MS.gene44157.t1
chr1.3 CDS 59247814 59248154 59247814 ID=cds.MS.gene44157.t1;Parent=MS.gene44157.t1
chr1.3 exon 59248274 59248534 59248274 ID=MS.gene44157.t1.exon4;Parent=MS.gene44157.t1
chr1.3 CDS 59248274 59248534 59248274 ID=cds.MS.gene44157.t1;Parent=MS.gene44157.t1
Gene Sequence

>MS.gene44157

ATGGTTTTCCAAAGGAGGGTATTCAACATCGCGGTCAACCCAAACTAGGTATGGTGCTCTAATCTTCAACAACCAATGATTCAAAATGCGATGCTAAAAAGTTCTTCAGAATGATTGTTTTTTTTGTTTCCCGATACACTTCCATATCTGGTGGATGTTGCATTGCATGCATTGATTCATCAGTGGCACAGTATATTGATGACTCTGATTATGTTATTTTTTATTTCCGTTTTTTTTTTCTTCTTAAATACAGATTGTTCAATATTGTAGTTGTAGATTGCATGCATTGATTCATCAGTGGCTCAGTATATTGATGACTCTGATTATGTTATTTATTTTTTCTTTTTTTTTATTTTGTTTCTTAAATACATATTGTTCAATGTTGTAGTTGTAGATTCCCTTATTTAAAAAAAAAAACCATAATTTTGTTTCTCATACACTTCCATTCTTTGTATTTTGTTGTGTTGTGTCAAATAAATGGTTATAATTACCAGTTTTTCTCTTTTTTGAAGGATTATTACTAGTTTTGTATTGCTCTTCTTTTTTGTATAATTTCTTCGAAAAACACAACATTAAATAATAATACCCTCGCCTTTGCTTATAGATTTTCAATACTTTTGGTTGTAAACATACTGTTGTTGTTGTTGTTGTTTGTATTCTGTAGCTTACGGCTCGTGTCCCATGGTATTTTTACGAACGTTTCAAAGGTCAAATAGAGCATAGAGTTTGGTTTCAGGATACCAGAGGGTCTAGGTTTGGAGTATGTTACGGAGAGATCAATGGCGGTGGTTGCTTCTATTCAGGTCTTCAGAACCTGCTTGATCATTATGAGCAACCATTAGGTCTAAACCTACGATTGACCTATGACGATAACGGGATGTTTCATATGCTTCCATTCACTCTTGACATGGATGAGGTAACTAATCGTCAATTGAGGAACTATGACCATAGCTCAAGGTTGCATGTCAATGAGTTCCTGCGTACATTTAGATACTTTGATTCCGACTCATCTTCGTCTTCGTCTTCGTCTTCGTCATCTGATGATGGCCAATTTCAAATTAATGCTGCTGCTATTGTGGGTTCTGGTGTTGCTGTTGGTGGTGATAATGGTGCCGTTGCTGCTGCTGATTTATTTCCAGCAGTTCCACCGGGTTTCGAAGTAGGAGGCCCGTCAAATCCAATTAATGATGCGCACAACTCTCCTCCCATTCCTGATGATGGTGATATGGTAGTTGTTCCTGACCCAGTACCAGTGGTGGAAGAACAAAATCCACAAACTGCCGCTGCGGTAGCCCAGATTCAGAACGTGGCTAATTTCTGGGATGATGATGAGGAACCTGCTTTCGATATTGGTGATAATGAGGAGGCTGCTATGGATGTCGAAATAGATGTGGTAGATGCTGAGGATGCACATGGCAATCCCCAGTTGGAGACACATGTTTTAACGCTTTCTGCGTACATGGTGAAATGCAGCAACATGGTGAGTTACTTTGAAAAAATAAAAAAAAAACATATCCACGCTTTATACAGTTATTTATTGTGGTTTGGCAAGTGTGTGACTGTTGACATGAATTAATTATTGTTATCTGTCAACTGCAGCTTGTACCAGCAAAATTTGCTCGGCGAGCATTTGGGAATACAAGACCAACCTTCATCACGATCCGAGATGAGTTCCACCGTGAGTATCCAATCGATATTAAGTGGAATGAAAAACCGCATAAAGCTACATCGGTATTTTTCAGGGGCGGTTGGGGTGAATTCGTTAGAGAGAACAGATTTGTGGAAGGCACAAGGGTGTTGCTCACTTTAAACAAGACTAACCCAACTGTCCTGCACGTGAGGCAGGTCGTTATGGTGTAA
Protein sequence

>MS.gene44157.t1

MVFQRRLTARVPWYFYERFKGQIEHRVWFQDTRGSRFGVCYGEINGGGCFYSGLQNLLDHYEQPLGLNLRLTYDDNGMFHMLPFTLDMDEVTNRQLRNYDHSSRLHVNEFLPVPPGFEVGGPSNPINDAHNSPPIPDDGDMVVVPDPVPVVEEQNPQTAAAVAQIQNVANFWDDDEEPAFDIGDNEEAAMDVEIDVVDAEDAHGNPQLETHVLTLSAYMVKCSNMLVPAKFARRAFGNTRPTFITIRDEFHREYPIDIKWNEKPHKATSVFFRGGWGEFVRENRFVEGTRVLLTLNKTNPTVLHVRQVVMV