Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44263.t1 | GAU23335.1 | 88.4 | 216 | 24 | 1 | 1 | 215 | 1 | 216 | 7.60E-95 | 356.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44263.t1 | P0CB25 | 46.2 | 210 | 92 | 6 | 4 | 204 | 38 | 235 | 2.2e-34 | 147.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44263.t1 | A0A2Z6MJD4 | 88.4 | 216 | 24 | 1 | 1 | 215 | 1 | 216 | 5.5e-95 | 356.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene44263.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44263.t1 | MTR_5g032375 | 87.907 | 215 | 18 | 2 | 8 | 215 | 1 | 214 | 2.95e-134 | 375 |
| MS.gene44263.t1 | MTR_5g066080 | 40.571 | 175 | 82 | 3 | 39 | 191 | 23 | 197 | 2.88e-26 | 102 |
| MS.gene44263.t1 | MTR_1g093750 | 37.126 | 167 | 87 | 3 | 50 | 205 | 156 | 315 | 1.12e-24 | 99.4 |
| MS.gene44263.t1 | MTR_3g070970 | 35.673 | 171 | 86 | 4 | 52 | 205 | 73 | 236 | 4.64e-23 | 94.0 |
| MS.gene44263.t1 | MTR_2g015890 | 37.589 | 141 | 80 | 2 | 41 | 180 | 56 | 189 | 5.53e-21 | 88.2 |
| MS.gene44263.t1 | MTR_2g091190 | 39.583 | 144 | 72 | 3 | 47 | 184 | 54 | 188 | 4.13e-20 | 85.5 |
| MS.gene44263.t1 | MTR_4g079760 | 35.256 | 156 | 90 | 2 | 41 | 192 | 57 | 205 | 1.35e-17 | 79.0 |
| MS.gene44263.t1 | MTR_2g015890 | 40.870 | 115 | 60 | 2 | 46 | 159 | 61 | 168 | 1.70e-17 | 78.2 |
| MS.gene44263.t1 | MTR_4g087920 | 36.364 | 143 | 78 | 4 | 46 | 182 | 68 | 203 | 3.10e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44263.t1 | AT4G38070 | 46.193 | 197 | 85 | 6 | 4 | 191 | 38 | 222 | 1.08e-46 | 155 |
| MS.gene44263.t1 | AT3G25710 | 41.611 | 149 | 77 | 3 | 39 | 184 | 119 | 260 | 1.34e-25 | 102 |
| MS.gene44263.t1 | AT1G68810 | 42.012 | 169 | 78 | 3 | 50 | 205 | 172 | 333 | 8.27e-25 | 100 |
| MS.gene44263.t1 | AT3G56770 | 33.333 | 174 | 93 | 6 | 47 | 207 | 39 | 202 | 3.65e-22 | 90.9 |
| MS.gene44263.t1 | AT2G40200 | 39.259 | 135 | 66 | 4 | 53 | 179 | 64 | 190 | 5.93e-20 | 85.5 |
| MS.gene44263.t1 | AT2G41130 | 35.838 | 173 | 93 | 5 | 46 | 207 | 60 | 225 | 1.42e-19 | 84.3 |
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGGCAGACAAATTGAATT+TGG | 0.253589 | 5.4:+22669996 | MS.gene44263:CDS |
| TGTCTGCCCCACCAGGAAAC+TGG | 0.346018 | 5.4:-22669984 | None:intergenic |
| TTGGACCCATAACACACTTC+TGG | 0.378699 | 5.4:-22670163 | None:intergenic |
| ACACTAACTTTGATCAAGTT+GGG | 0.389149 | 5.4:-22669610 | None:intergenic |
| AGGTGTTGAAAATGAAGGTT+TGG | 0.393224 | 5.4:+22670185 | MS.gene44263:CDS |
| AATGAAAGCCAAAGTTGTTA+AGG | 0.399548 | 5.4:+22670113 | MS.gene44263:CDS |
| GAAGAACCAGAAGTGTGTTA+TGG | 0.408049 | 5.4:+22670157 | MS.gene44263:CDS |
| CATCTCAACCTTAACAACTT+TGG | 0.408942 | 5.4:-22670121 | None:intergenic |
| AAGAACCAGAAGTGTGTTAT+GGG | 0.427563 | 5.4:+22670158 | MS.gene44263:CDS |
| TCAAAGGAGATCCAGTTTCC+TGG | 0.436396 | 5.4:+22669973 | MS.gene44263:CDS |
| GACACTAACTTTGATCAAGT+TGG | 0.443559 | 5.4:-22669611 | None:intergenic |
| GATGAGGCGGTATACTCAAT+AGG | 0.446628 | 5.4:+22670257 | MS.gene44263:CDS |
| GGTGTTGAAAATGAAGGTTT+GGG | 0.450241 | 5.4:+22670186 | MS.gene44263:CDS |
| TTCAATTTGTCTGCCCCACC+AGG | 0.456050 | 5.4:-22669991 | None:intergenic |
| TACCGCCTCATCTTAAAACT+TGG | 0.469951 | 5.4:-22670246 | None:intergenic |
| GATATAGATGACATCAATCC+TGG | 0.505784 | 5.4:-22670075 | None:intergenic |
| AGACGTAGAATGCGAATCAA+TGG | 0.510341 | 5.4:+22669559 | MS.gene44263:CDS |
| AGGAAACTGGATCTCCTTTG+AGG | 0.538324 | 5.4:-22669971 | None:intergenic |
| CAAAGTTGTTAAGGTTGAGA+TGG | 0.542500 | 5.4:+22670122 | MS.gene44263:CDS |
| GTCCAAGGTGTTGAAAATGA+AGG | 0.548376 | 5.4:+22670180 | MS.gene44263:CDS |
| GGAAAAGTGTAATGATGAGG+AGG | 0.576454 | 5.4:+22670017 | MS.gene44263:CDS |
| TTGAGTTGTGAGGATAGACC+AGG | 0.582755 | 5.4:+22670057 | MS.gene44263:CDS |
| GGAGATCCAGTTTCCTGGTG+GGG | 0.584829 | 5.4:+22669978 | MS.gene44263:CDS |
| GGATCTCCTTTGAGGTAGCA+TGG | 0.586127 | 5.4:-22669963 | None:intergenic |
| AGAAGTGTGTTATGGGTCCA+AGG | 0.587934 | 5.4:+22670165 | MS.gene44263:CDS |
| CATGTTCGAAATTACTGTAA+CGG | 0.590992 | 5.4:+22669403 | MS.gene44263:CDS |
| CACTAACTTTGATCAAGTTG+GGG | 0.594450 | 5.4:-22669609 | None:intergenic |
| GGAGACTATCAAACAAGTGA+AGG | 0.601788 | 5.4:+22669903 | MS.gene44263:CDS |
| AGGAGATCCAGTTTCCTGGT+GGG | 0.605957 | 5.4:+22669977 | MS.gene44263:CDS |
| GTTGAGATGGTGACAGTGGG+TGG | 0.609342 | 5.4:+22670135 | MS.gene44263:CDS |
| GAAAAGTGTAATGATGAGGA+GGG | 0.610839 | 5.4:+22670018 | MS.gene44263:CDS |
| TAAGGTTGAGATGGTGACAG+TGG | 0.619246 | 5.4:+22670131 | MS.gene44263:CDS |
| TAACTTTGATCAAGTTGGGG+AGG | 0.625634 | 5.4:-22669606 | None:intergenic |
| GGACAAGGCATCAATACTAG+TGG | 0.628919 | 5.4:+22669882 | MS.gene44263:CDS |
| TATCTCGAAGCAAAGATCAA+AGG | 0.632574 | 5.4:+22669489 | MS.gene44263:CDS |
| AGATAGCCATGCTACCTCAA+AGG | 0.632769 | 5.4:+22669957 | MS.gene44263:CDS |
| TATTGATTGACAGAAGGACA+AGG | 0.644249 | 5.4:+22669867 | MS.gene44263:intron |
| AAGGTTGAGATGGTGACAGT+GGG | 0.645311 | 5.4:+22670132 | MS.gene44263:CDS |
| AAGGAGATCCAGTTTCCTGG+TGG | 0.648546 | 5.4:+22669976 | MS.gene44263:CDS |
| CTCGAAGCAAAGATCAAAGG+CGG | 0.651214 | 5.4:+22669492 | MS.gene44263:CDS |
| AGTTGGGGAGGATACTGCGG+AGG | 0.655539 | 5.4:-22669594 | None:intergenic |
| TTTGGAAAAGTGTAATGATG+AGG | 0.678742 | 5.4:+22670014 | MS.gene44263:CDS |
| GAAAGCTACATTGAGTTGTG+AGG | 0.689461 | 5.4:+22670047 | MS.gene44263:CDS |
| TCAAGTTGGGGAGGATACTG+CGG | 0.696028 | 5.4:-22669597 | None:intergenic |
| GTTGGGGAGGATACTGCGGA+GGG | 0.717546 | 5.4:-22669593 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGTTTTCATAAAGT+TGG | - | chr5.4:22669654-22669673 | None:intergenic | 15.0% |
| !! | AACAAATTCTAAATTTAGCA+AGG | - | chr5.4:22669848-22669867 | None:intergenic | 20.0% |
| !!! | TTATACTTTTGTGTATTACA+AGG | + | chr5.4:22669675-22669694 | MS.gene44263:intron | 20.0% |
| !!! | TTTTAGTAAAACACAATGAA+AGG | - | chr5.4:22669360-22669379 | None:intergenic | 20.0% |
| ! | AATGGTTAAATATGTTGATG+TGG | + | chr5.4:22669743-22669762 | MS.gene44263:intron | 25.0% |
| ! | ACTAAAATAGTTAGAACACA+GGG | + | chr5.4:22669373-22669392 | MS.gene44263:intron | 25.0% |
| ! | ACTATTATTGTAAGGAAGAA+TGG | + | chr5.4:22669725-22669744 | MS.gene44263:intron | 25.0% |
| ! | TAAATTTAGCAAGGTTGTTT+AGG | - | chr5.4:22669839-22669858 | None:intergenic | 25.0% |
| ! | TACTAAAATAGTTAGAACAC+AGG | + | chr5.4:22669372-22669391 | MS.gene44263:intron | 25.0% |
| !!! | ATTTGTTATTGATTGACAGA+AGG | + | chr5.4:22669861-22669880 | MS.gene44263:intron | 25.0% |
| AATGAAAGCCAAAGTTGTTA+AGG | + | chr5.4:22670113-22670132 | MS.gene44263:CDS | 30.0% | |
| ACACTAACTTTGATCAAGTT+GGG | - | chr5.4:22669613-22669632 | None:intergenic | 30.0% | |
| CATGTTCGAAATTACTGTAA+CGG | + | chr5.4:22669403-22669422 | MS.gene44263:CDS | 30.0% | |
| TTGCGATCACTATTATTGTA+AGG | + | chr5.4:22669717-22669736 | MS.gene44263:intron | 30.0% | |
| ! | TTTGGAAAAGTGTAATGATG+AGG | + | chr5.4:22670014-22670033 | MS.gene44263:CDS | 30.0% |
| !!! | ATAAGCCAAGTTTTAAGATG+AGG | + | chr5.4:22670241-22670260 | MS.gene44263:CDS | 30.0% |
| CAAAGTTGTTAAGGTTGAGA+TGG | + | chr5.4:22670122-22670141 | MS.gene44263:CDS | 35.0% | |
| CATCTCAACCTTAACAACTT+TGG | - | chr5.4:22670124-22670143 | None:intergenic | 35.0% | |
| GACACTAACTTTGATCAAGT+TGG | - | chr5.4:22669614-22669633 | None:intergenic | 35.0% | |
| GATATAGATGACATCAATCC+TGG | - | chr5.4:22670078-22670097 | None:intergenic | 35.0% | |
| TATCTCGAAGCAAAGATCAA+AGG | + | chr5.4:22669489-22669508 | MS.gene44263:CDS | 35.0% | |
| ! | AACCTTCATTTTCAACACCT+TGG | - | chr5.4:22670185-22670204 | None:intergenic | 35.0% |
| ! | AAGAACCAGAAGTGTGTTAT+GGG | + | chr5.4:22670158-22670177 | MS.gene44263:CDS | 35.0% |
| ! | CACTAACTTTGATCAAGTTG+GGG | - | chr5.4:22669612-22669631 | None:intergenic | 35.0% |
| ! | GAAAAGTGTAATGATGAGGA+GGG | + | chr5.4:22670018-22670037 | MS.gene44263:CDS | 35.0% |
| ! | GGTGTTGAAAATGAAGGTTT+GGG | + | chr5.4:22670186-22670205 | MS.gene44263:CDS | 35.0% |
| ! | TATTGATTGACAGAAGGACA+AGG | + | chr5.4:22669867-22669886 | MS.gene44263:intron | 35.0% |
| !! | AGGTGTTGAAAATGAAGGTT+TGG | + | chr5.4:22670185-22670204 | MS.gene44263:CDS | 35.0% |
| AGACGTAGAATGCGAATCAA+TGG | + | chr5.4:22669559-22669578 | MS.gene44263:CDS | 40.0% | |
| GGAGACTATCAAACAAGTGA+AGG | + | chr5.4:22669903-22669922 | MS.gene44263:CDS | 40.0% | |
| TACCGCCTCATCTTAAAACT+TGG | - | chr5.4:22670249-22670268 | None:intergenic | 40.0% | |
| TCACGATGCAGTCTAAATCA+TGG | + | chr5.4:22669778-22669797 | MS.gene44263:intron | 40.0% | |
| TGGGGCAGACAAATTGAATT+TGG | + | chr5.4:22669996-22670015 | MS.gene44263:CDS | 40.0% | |
| ! | GAAAGCTACATTGAGTTGTG+AGG | + | chr5.4:22670047-22670066 | MS.gene44263:CDS | 40.0% |
| ! | GAAGAACCAGAAGTGTGTTA+TGG | + | chr5.4:22670157-22670176 | MS.gene44263:CDS | 40.0% |
| ! | GGAAAAGTGTAATGATGAGG+AGG | + | chr5.4:22670017-22670036 | MS.gene44263:CDS | 40.0% |
| !! | GTCCAAGGTGTTGAAAATGA+AGG | + | chr5.4:22670180-22670199 | MS.gene44263:CDS | 40.0% |
| !! | TAACTTTGATCAAGTTGGGG+AGG | - | chr5.4:22669609-22669628 | None:intergenic | 40.0% |
| !!! | AGCCAAGTTTTAAGATGAGG+CGG | + | chr5.4:22670244-22670263 | MS.gene44263:CDS | 40.0% |
| AAGGTTGAGATGGTGACAGT+GGG | + | chr5.4:22670132-22670151 | MS.gene44263:CDS | 45.0% | |
| AGATAGCCATGCTACCTCAA+AGG | + | chr5.4:22669957-22669976 | MS.gene44263:CDS | 45.0% | |
| AGGAAACTGGATCTCCTTTG+AGG | - | chr5.4:22669974-22669993 | None:intergenic | 45.0% | |
| CTCGAAGCAAAGATCAAAGG+CGG | + | chr5.4:22669492-22669511 | MS.gene44263:CDS | 45.0% | |
| TAAGGTTGAGATGGTGACAG+TGG | + | chr5.4:22670131-22670150 | MS.gene44263:CDS | 45.0% | |
| TCAAAGGAGATCCAGTTTCC+TGG | + | chr5.4:22669973-22669992 | MS.gene44263:CDS | 45.0% | |
| TTGGACCCATAACACACTTC+TGG | - | chr5.4:22670166-22670185 | None:intergenic | 45.0% | |
| ! | AGAAGTGTGTTATGGGTCCA+AGG | + | chr5.4:22670165-22670184 | MS.gene44263:CDS | 45.0% |
| ! | GGACAAGGCATCAATACTAG+TGG | + | chr5.4:22669882-22669901 | MS.gene44263:CDS | 45.0% |
| ! | TTGAGTTGTGAGGATAGACC+AGG | + | chr5.4:22670057-22670076 | MS.gene44263:CDS | 45.0% |
| AAGGAGATCCAGTTTCCTGG+TGG | + | chr5.4:22669976-22669995 | MS.gene44263:CDS | 50.0% | |
| AGGAGATCCAGTTTCCTGGT+GGG | + | chr5.4:22669977-22669996 | MS.gene44263:CDS | 50.0% | |
| TTCAATTTGTCTGCCCCACC+AGG | - | chr5.4:22669994-22670013 | None:intergenic | 50.0% | |
| !! | GGATCTCCTTTGAGGTAGCA+TGG | - | chr5.4:22669966-22669985 | None:intergenic | 50.0% |
| !! | TCAAGTTGGGGAGGATACTG+CGG | - | chr5.4:22669600-22669619 | None:intergenic | 50.0% |
| GGAGATCCAGTTTCCTGGTG+GGG | + | chr5.4:22669978-22669997 | MS.gene44263:CDS | 55.0% | |
| GTTGAGATGGTGACAGTGGG+TGG | + | chr5.4:22670135-22670154 | MS.gene44263:CDS | 55.0% | |
| AGTTGGGGAGGATACTGCGG+AGG | - | chr5.4:22669597-22669616 | None:intergenic | 60.0% | |
| GTTGGGGAGGATACTGCGGA+GGG | - | chr5.4:22669596-22669615 | None:intergenic | 60.0% | |
| TGTCTGCCCCACCAGGAAAC+TGG | - | chr5.4:22669987-22670006 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 22669302 | 22670278 | 22669302 | ID=MS.gene44263 |
| chr5.4 | mRNA | 22669302 | 22670278 | 22669302 | ID=MS.gene44263.t1;Parent=MS.gene44263 |
| chr5.4 | exon | 22669302 | 22669319 | 22669302 | ID=MS.gene44263.t1.exon1;Parent=MS.gene44263.t1 |
| chr5.4 | CDS | 22669302 | 22669319 | 22669302 | ID=cds.MS.gene44263.t1;Parent=MS.gene44263.t1 |
| chr5.4 | exon | 22669394 | 22669624 | 22669394 | ID=MS.gene44263.t1.exon2;Parent=MS.gene44263.t1 |
| chr5.4 | CDS | 22669394 | 22669624 | 22669394 | ID=cds.MS.gene44263.t1;Parent=MS.gene44263.t1 |
| chr5.4 | exon | 22669880 | 22670278 | 22669880 | ID=MS.gene44263.t1.exon3;Parent=MS.gene44263.t1 |
| chr5.4 | CDS | 22669880 | 22670278 | 22669880 | ID=cds.MS.gene44263.t1;Parent=MS.gene44263.t1 |
| Gene Sequence |
| Protein sequence |
