AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44415.t1 MTR_0246s0020 92.651 381 27 1 1 381 1 380 0.0 657
MS.gene44415.t1 MTR_0246s0020 89.516 372 36 2 1 372 1 369 0.0 608
MS.gene44415.t1 MTR_6g047570 57.326 389 104 8 1 380 1 336 6.01e-135 389
MS.gene44415.t1 MTR_0011s0260 58.612 389 121 10 1 379 1 359 7.57e-127 369
MS.gene44415.t1 MTR_0011s0210 58.312 391 123 10 1 381 1 361 6.56e-126 367
MS.gene44415.t1 MTR_4g067010 52.972 387 105 10 1 378 1 319 5.52e-115 338
MS.gene44415.t1 MTR_4g098035 50.758 396 125 14 1 380 1 342 1.22e-108 322
MS.gene44415.t1 MTR_4g066460 53.213 389 113 10 1 380 1 329 3.79e-107 318
MS.gene44415.t1 MTR_4g066380 50.895 391 120 9 1 382 1 328 1.52e-104 311
MS.gene44415.t1 MTR_0246s0050 49.598 373 120 12 23 380 2 321 1.73e-99 298
MS.gene44415.t1 MTR_0250s0040 48.747 359 88 9 24 380 1 265 7.01e-91 274
MS.gene44415.t1 MTR_6g047550 57.091 275 73 7 1 265 1 240 3.73e-88 268
MS.gene44415.t1 MTR_5g014520 51.154 260 99 6 121 379 101 333 4.19e-67 216
MS.gene44415.t1 MTR_5g014560 49.174 242 96 7 147 379 84 307 3.40e-59 194
MS.gene44415.t1 MTR_5g014600 36.074 377 179 13 13 375 6 334 5.42e-53 179
MS.gene44415.t1 MTR_4g009540 49.206 189 91 3 191 376 145 331 7.50e-53 179
MS.gene44415.t1 MTR_2g010450 49.462 186 84 4 196 378 175 353 9.61e-53 179
MS.gene44415.t1 MTR_4g009110 39.925 268 124 5 116 376 113 350 2.68e-49 170
MS.gene44415.t1 MTR_2g104490 39.773 264 130 6 108 370 67 302 1.43e-48 167
MS.gene44415.t1 MTR_8g009020 38.603 272 126 8 111 371 77 318 5.03e-45 158
MS.gene44415.t1 MTR_4g009540 49.405 168 80 3 191 355 145 310 1.19e-44 156
MS.gene44415.t1 MTR_4g097940 47.090 189 87 6 185 370 142 320 7.71e-44 155
MS.gene44415.t1 MTR_4g092700 43.216 199 104 3 182 378 142 333 9.35e-44 155
MS.gene44415.t1 MTR_2g104550 38.462 273 142 6 108 379 68 315 1.71e-43 154
MS.gene44415.t1 MTR_4g097920 45.503 189 90 6 185 370 127 305 1.79e-43 153
MS.gene44415.t1 MTR_4g097920 45.503 189 90 6 185 370 142 320 2.47e-43 154
MS.gene44415.t1 MTR_4g097950 41.065 263 127 9 120 377 12 251 4.89e-43 150
MS.gene44415.t1 MTR_2g104490 38.554 249 124 6 108 355 67 287 5.86e-41 146
MS.gene44415.t1 MTR_5g014640 35.241 332 156 13 68 375 27 323 1.12e-40 146
MS.gene44415.t1 MTR_8g069740 37.745 204 115 4 153 354 72 265 2.21e-39 141
MS.gene44415.t1 MTR_2g104560 37.615 218 111 6 167 377 27 226 3.47e-39 140
MS.gene44415.t1 MTR_2g104650 39.450 218 127 3 159 374 53 267 1.38e-38 139
MS.gene44415.t1 MTR_7g080780 31.437 334 178 8 74 383 26 332 1.93e-37 138
MS.gene44415.t1 MTR_7g080780 31.437 334 178 8 74 383 13 319 2.25e-37 137
MS.gene44415.t1 MTR_2g104500 33.692 279 125 8 106 376 46 272 2.96e-32 122
MS.gene44415.t1 MTR_8g009010 33.453 278 118 7 99 371 52 267 2.00e-31 121
MS.gene44415.t1 MTR_2g104530 35.784 204 113 4 169 371 83 269 4.30e-31 120
MS.gene44415.t1 MTR_2g104590 33.778 225 110 7 161 374 26 222 8.99e-30 115
MS.gene44415.t1 MTR_4g009510 40.491 163 63 5 221 373 64 202 5.61e-27 106
MS.gene44415.t1 MTR_7g451650 38.272 162 67 4 221 373 57 194 4.14e-25 101
MS.gene44415.t1 MTR_5g014550 48.062 129 58 4 228 354 54 175 2.16e-23 97.1
MS.gene44415.t1 MTR_7g050350 37.179 156 65 4 227 373 1 132 2.85e-23 94.7
MS.gene44415.t1 MTR_7g451650 37.179 156 65 4 227 373 1 132 2.85e-23 94.7
MS.gene44415.t1 MTR_4g009420 36.646 161 63 4 221 373 65 194 3.17e-23 96.3
MS.gene44415.t1 MTR_8g067280 34.146 164 93 3 190 340 448 609 1.31e-17 85.1
MS.gene44415.t1 MTR_1g072320 31.183 186 108 6 198 370 470 648 1.68e-17 84.7
MS.gene44415.t1 MTR_7g451650 35.417 144 60 4 221 355 57 176 3.20e-16 76.6
MS.gene44415.t1 MTR_8g024790 30.201 149 87 5 198 340 291 428 8.20e-16 79.0
MS.gene44415.t1 MTR_5g030430 31.111 180 109 3 190 356 479 656 2.31e-15 78.2
MS.gene44415.t1 MTR_8g027495 48.438 64 33 0 198 261 458 521 7.62e-14 73.6
MS.gene44415.t1 MTR_7g117670 53.571 56 26 0 198 253 402 457 1.13e-13 72.8
MS.gene44415.t1 MTR_7g096350 48.529 68 29 1 190 251 341 408 2.01e-13 72.0
MS.gene44415.t1 MTR_8g064700 25.444 169 112 4 191 352 237 398 2.74e-13 71.2
MS.gene44415.t1 MTR_8g024770 33.051 118 71 3 198 312 279 391 3.64e-13 70.9
MS.gene44415.t1 MTR_1g112680 24.713 174 107 4 190 340 309 481 1.38e-12 69.3
MS.gene44415.t1 MTR_3g030700 26.554 177 126 3 180 354 178 352 2.36e-12 68.2
MS.gene44415.t1 MTR_3g498695 24.339 189 138 3 183 366 283 471 1.27e-11 66.2
MS.gene44415.t1 MTR_7g110810 46.875 64 34 0 187 250 201 264 4.03e-11 64.3
MS.gene44415.t1 MTR_1g019240 36.538 104 63 2 153 254 49 151 4.86e-11 63.2
MS.gene44415.t1 MTR_3g498695 25.161 155 112 2 183 333 283 437 5.37e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44415.t1 AT4G37850 38.144 291 143 10 101 378 62 328 9.12e-50 171
MS.gene44415.t1 AT4G37850 38.144 291 143 10 101 378 62 328 9.12e-50 171
MS.gene44415.t1 AT2G22750 36.508 378 185 15 7 377 3 332 1.05e-43 155
MS.gene44415.t1 AT2G22750 45.408 196 105 2 183 377 110 304 1.12e-43 154
MS.gene44415.t1 AT2G22750 45.408 196 105 2 183 377 134 328 1.54e-43 154
MS.gene44415.t1 AT2G22750 45.408 196 104 3 183 377 110 303 2.68e-42 150
MS.gene44415.t1 AT2G22750 36.508 378 184 16 7 377 3 331 3.37e-42 150
MS.gene44415.t1 AT2G22760 41.753 194 104 5 186 375 107 295 7.00e-40 144
MS.gene44415.t1 AT2G22760 41.040 173 94 4 186 355 107 274 2.47e-33 126
MS.gene44415.t1 AT2G22770 34.211 304 158 11 82 373 44 317 3.57e-30 118
MS.gene44415.t1 AT4G17880 31.818 176 102 3 190 356 402 568 7.64e-16 79.7
MS.gene44415.t1 AT5G46760 29.648 199 104 7 190 359 401 592 1.01e-15 79.3
MS.gene44415.t1 AT1G32640 32.903 155 83 4 190 333 438 582 2.10e-15 78.2
MS.gene44415.t1 AT1G01260 35.772 123 51 3 192 314 427 521 9.02e-15 76.3
MS.gene44415.t1 AT1G01260 35.772 123 51 3 192 314 427 521 9.02e-15 76.3
MS.gene44415.t1 AT1G01260 35.772 123 51 3 192 314 427 521 9.02e-15 76.3
MS.gene44415.t1 AT4G16430 50.746 67 33 0 192 258 314 380 1.29e-14 75.5
MS.gene44415.t1 AT2G46510 45.946 74 34 1 190 257 381 454 1.95e-14 75.1
MS.gene44415.t1 AT1G63650 27.848 158 82 4 198 333 405 552 2.82e-13 71.6
MS.gene44415.t1 AT1G63650 27.848 158 82 4 198 333 405 552 2.82e-13 71.6
MS.gene44415.t1 AT1G63650 27.848 158 82 4 198 333 405 552 2.82e-13 71.6
MS.gene44415.t1 AT4G09820 29.814 161 73 4 189 340 352 481 7.20e-13 70.5
MS.gene44415.t1 AT2G16910 28.313 166 93 4 199 343 315 475 1.69e-12 69.3
MS.gene44415.t1 AT4G00870 33.333 117 75 1 199 315 250 363 2.84e-12 68.2
MS.gene44415.t1 AT5G41315 23.980 196 127 4 192 368 435 627 4.67e-12 67.8
MS.gene44415.t1 AT5G41315 23.980 196 127 4 192 368 443 635 4.70e-12 67.8
MS.gene44415.t1 AT5G41315 23.980 196 127 4 192 368 440 632 4.78e-12 67.8
MS.gene44415.t1 AT5G57150 28.655 171 105 4 196 356 53 216 1.69e-11 63.9
MS.gene44415.t1 AT5G57150 28.824 170 104 4 196 355 53 215 2.83e-11 63.2
MS.gene44415.t1 AT5G57150 29.070 172 105 4 196 357 52 216 3.21e-11 63.5
MS.gene44415.t1 AT5G57150 28.824 170 104 4 196 355 52 214 3.59e-11 62.8
MS.gene44415.t1 AT5G57150 28.824 170 104 4 196 355 53 215 3.63e-11 63.5
MS.gene44415.t1 AT4G29930 26.286 175 112 5 199 357 55 228 5.61e-11 62.8
MS.gene44415.t1 AT4G29930 26.012 173 111 5 199 355 55 226 6.97e-11 62.8
MS.gene44415.t1 AT4G29930 26.012 173 111 5 199 355 55 226 6.97e-11 62.8
MS.gene44415.t1 AT2G28160 26.220 164 110 3 179 341 117 270 8.67e-11 62.8
MS.gene44415.t1 AT2G28160 26.220 164 110 3 179 341 117 270 9.07e-11 62.8

Find 65 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8.2:-24090334 None:intergenic
GTGTTGGTAGGAGGAGAATT+TGG 0.206656 8.2:-24089497 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8.2:+24089067 MS.gene44415:CDS
GACATGAATGTTTCTCATTT+GGG 0.281293 8.2:-24089594 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8.2:-24089595 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8.2:-24089217 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8.2:-24089203 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8.2:+24090334 MS.gene44415:CDS
TGTTGGTAGGAGGAGAATTT+GGG 0.316269 8.2:-24089496 None:intergenic
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8.2:+24089769 MS.gene44415:CDS
GTTTCTTCTGATACGTTCAT+TGG 0.319465 8.2:-24089044 None:intergenic
CAGGAGTGAGTCCACAAAAC+TGG 0.341350 8.2:-24089523 None:intergenic
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8.2:+24090402 MS.gene44415:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8.2:-24089066 None:intergenic
GGCGTTGGCTCGAGACCTTT+TGG 0.371700 8.2:-24089678 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8.2:-24089431 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8.2:+24090401 MS.gene44415:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8.2:-24089706 None:intergenic
CTGAACTCAAACATTGTTGT+TGG 0.389224 8.2:-24089268 None:intergenic
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8.2:-24089745 None:intergenic
CATCATCATCAATGATACTA+TGG 0.407350 8.2:-24090199 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8.2:+24089614 MS.gene44415:CDS
GTTGGTGATAGTTGTGGAAT+TGG 0.425475 8.2:-24089294 None:intergenic
TGAACTCAAACATTGTTGTT+GGG 0.428381 8.2:-24089267 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8.2:+24090303 MS.gene44415:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8.2:+24090284 MS.gene44415:CDS
AGGAGTGAGTCCACAAAACT+GGG 0.451243 8.2:-24089522 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8.2:+24089615 MS.gene44415:CDS
TCATATGATGAGAGTATTGA+AGG 0.466240 8.2:+24090242 MS.gene44415:CDS
TGTTGTTGGGTTTGTTCTTG+TGG 0.480560 8.2:-24089254 None:intergenic
TGACATTACTATCATTGCTC+AGG 0.494765 8.2:+24090433 MS.gene44415:CDS
AGCAATGATAGTAATGTCAA+TGG 0.495292 8.2:-24090429 None:intergenic
TTTGACTTTGAGTTTAAGGC+AGG 0.501893 8.2:-24089542 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8.2:-24089699 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8.2:-24089693 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8.2:+24089220 MS.gene44415:CDS
TCATCAATGATACTATGGTC+TGG 0.549545 8.2:-24090194 None:intergenic
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8.2:-24089226 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8.2:+24089054 MS.gene44415:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8.2:-24089590 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8.2:+24089694 MS.gene44415:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8.2:-24089443 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8.2:+24090403 MS.gene44415:CDS
TAAAATTTGGTAGATGGACA+AGG 0.590351 8.2:+24090058 MS.gene44415:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8.2:-24090416 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8.2:+24091409 MS.gene44415:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8.2:-24091445 None:intergenic
TGAAATTGAGAGACCAACCA+TGG 0.603140 8.2:+24089361 MS.gene44415:CDS
TGGTAGGAGGAGAATTTGGG+AGG 0.603292 8.2:-24089493 None:intergenic
GAATGAGTTGGTGATAGTTG+TGG 0.603767 8.2:-24089300 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8.2:+24090346 MS.gene44415:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8.2:+24090325 MS.gene44415:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8.2:+24090327 MS.gene44415:CDS
AATGTCAATGGAATCCCCAA+AGG 0.614554 8.2:-24090417 None:intergenic
TGTTTCATCAGTGAATGAGT+TGG 0.619624 8.2:-24089312 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8.2:-24089593 None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8.2:+24090285 MS.gene44415:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8.2:+24090157 MS.gene44415:CDS
GAGTGAGTCCACAAAACTGG+GGG 0.649560 8.2:-24089520 None:intergenic
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8.2:-24090174 None:intergenic
GGAGTGAGTCCACAAAACTG+GGG 0.660208 8.2:-24089521 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8.2:+24090326 MS.gene44415:CDS
AGAACAAAACAAGAAAACAA+AGG 0.679620 8.2:+24090145 MS.gene44415:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8.2:+24091433 MS.gene44415:CDS
AGTGAGTCCACAAAACTGGG+GGG 0.773710 8.2:-24089519 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAGTATAGAAATAATTTAT+TGG + chr8.2:24090992-24091011 MS.gene44415:intron 15.0%
!! TCAAATACTGTTATAAAATT+TGG + chr8.2:24090045-24090064 MS.gene44415:intron 15.0%
!! TTTAAACAATGAATATTTAG+TGG - chr8.2:24090491-24090510 None:intergenic 15.0%
!!! AACTTGTTAAGAATAAAAAA+AGG + chr8.2:24090617-24090636 MS.gene44415:intron 15.0%
!!! AAGTATTATTATTTTGTTTG+AGG + chr8.2:24090546-24090565 MS.gene44415:intron 15.0%
!!! ATTAAATGCACAAAATTTTA+AGG + chr8.2:24091053-24091072 MS.gene44415:intron 15.0%
!!! ATTATTACATTTTTTGTCTA+GGG + chr8.2:24090780-24090799 MS.gene44415:intron 15.0%
!! AAGGAATTTATTACCATAAA+AGG + chr8.2:24090636-24090655 MS.gene44415:intron 20.0%
!! ATAATTAATGAAATGCTAAC+CGG - chr8.2:24090753-24090772 None:intergenic 20.0%
!! ATATTTAGTGGCAATTATTA+AGG - chr8.2:24090479-24090498 None:intergenic 20.0%
!!! AACTTGTGTTGTTTTTTTTT+TGG + chr8.2:24090929-24090948 MS.gene44415:intron 20.0%
!!! ATTGTTATACTTTTGTAGAT+GGG + chr8.2:24091383-24091402 MS.gene44415:intron 20.0%
!!! CACTACTTTTTTTTTATATC+AGG + chr8.2:24091128-24091147 MS.gene44415:intron 20.0%
!!! CATTATTACATTTTTTGTCT+AGG + chr8.2:24090779-24090798 MS.gene44415:intron 20.0%
!!! TAATTTTTTTTTGTTGTTGC+AGG + chr8.2:24089157-24089176 MS.gene44415:intron 20.0%
!!! TATTGTTATACTTTTGTAGA+TGG + chr8.2:24091382-24091401 MS.gene44415:intron 20.0%
!!! TCTTTTTAGGAATTTTGTTT+AGG + chr8.2:24091291-24091310 MS.gene44415:intron 20.0%
!!! TGATTTGGTAAGAAAATTAA+AGG + chr8.2:24089082-24089101 MS.gene44415:intron 20.0%
!!! TTATTACATTTTTTGTCTAG+GGG + chr8.2:24090781-24090800 MS.gene44415:intron 20.0%
!!! TTTTCTATCTGTTTCTTTTT+AGG + chr8.2:24091278-24091297 MS.gene44415:intron 20.0%
! AATACAATTAAACAACTCGT+GGG - chr8.2:24091355-24091374 None:intergenic 25.0%
! AGAACAAAACAAGAAAACAA+AGG + chr8.2:24090145-24090164 MS.gene44415:CDS 25.0%
! AGTAGTGAAAAAATGTTTAG+TGG - chr8.2:24090522-24090541 None:intergenic 25.0%
! CTGTTATAAAATTTGGTAGA+TGG + chr8.2:24090052-24090071 MS.gene44415:intron 25.0%
! TAATACAATTAAACAACTCG+TGG - chr8.2:24091356-24091375 None:intergenic 25.0%
! TAATTAATGAAATGCTAACC+GGG - chr8.2:24090752-24090771 None:intergenic 25.0%
! TATTACACGTATTCATGAAA+GGG + chr8.2:24091232-24091251 MS.gene44415:intron 25.0%
! TTATTACACGTATTCATGAA+AGG + chr8.2:24091231-24091250 MS.gene44415:intron 25.0%
!! TAAGGAATACTTCTTCTTTT+GGG + chr8.2:24091071-24091090 MS.gene44415:intron 25.0%
!! TGTTGGCTGTTATAATTAAT+TGG - chr8.2:24091187-24091206 None:intergenic 25.0%
!! TTAAGGAATACTTCTTCTTT+TGG + chr8.2:24091070-24091089 MS.gene44415:intron 25.0%
!!! AAAGTATCGTCAATAATTGT+TGG - chr8.2:24091204-24091223 None:intergenic 25.0%
!!! AATAATTAACTTGTGCCTTA+AGG - chr8.2:24090972-24090991 None:intergenic 25.0%
!!! ATAATTAACTTGTGCCTTAA+GGG - chr8.2:24090971-24090990 None:intergenic 25.0%
AAAGATGAGTTAGATGATGA+TGG - chr8.2:24089446-24089465 None:intergenic 30.0%
AATATCATTAATCAATGCCC+CGG + chr8.2:24090731-24090750 MS.gene44415:intron 30.0%
AATTAATGAAATGCTAACCG+GGG - chr8.2:24090751-24090770 None:intergenic 30.0%
ACATGAATGTTTCTCATTTG+GGG - chr8.2:24089596-24089615 None:intergenic 30.0%
AGACATGAATGTTTCTCATT+TGG - chr8.2:24089598-24089617 None:intergenic 30.0%
AGCAATGATAGTAATGTCAA+TGG - chr8.2:24090432-24090451 None:intergenic 30.0%
ATTATTAGGGTTATGCTAAC+CGG + chr8.2:24090584-24090603 MS.gene44415:intron 30.0%
CAAAAACATCTCAAACCAAA+AGG + chr8.2:24089663-24089682 MS.gene44415:CDS 30.0%
CATCATCATCAATGATACTA+TGG - chr8.2:24090202-24090221 None:intergenic 30.0%
GACATGAATGTTTCTCATTT+GGG - chr8.2:24089597-24089616 None:intergenic 30.0%
TAAAATTTGGTAGATGGACA+AGG + chr8.2:24090058-24090077 MS.gene44415:intron 30.0%
TCTCAACTCAAAATCCTAAA+GGG + chr8.2:24089615-24089634 MS.gene44415:CDS 30.0%
TTAGTGGCAATTATTAAGGT+AGG - chr8.2:24090475-24090494 None:intergenic 30.0%
! AAGGAATACTTCTTCTTTTG+GGG + chr8.2:24091072-24091091 MS.gene44415:intron 30.0%
! GTTAACGAAGTTTCCTTTTA+TGG - chr8.2:24090652-24090671 None:intergenic 30.0%
! TGAACTCAAACATTGTTGTT+GGG - chr8.2:24089270-24089289 None:intergenic 30.0%
!! TCATATGATGAGAGTATTGA+AGG + chr8.2:24090242-24090261 MS.gene44415:CDS 30.0%
!!! AACATTGATTTTGAAAGCGA+GGG - chr8.2:24090828-24090847 None:intergenic 30.0%
!!! ACTTTTGACTCTTTTGACTT+TGG + chr8.2:24089335-24089354 MS.gene44415:CDS 30.0%
!!! CTGTTTTGACTTTGAGTTTA+AGG - chr8.2:24089549-24089568 None:intergenic 30.0%
!!! TAAAAAGCGTTTGGAAGTTT+TGG + chr8.2:24090121-24090140 MS.gene44415:CDS 30.0%
!!! TGTTGCAAAGTTTTGAGTTT+TGG - chr8.2:24089645-24089664 None:intergenic 30.0%
!!! TTTTTTAACATGTGCCCTAA+GGG + chr8.2:24091095-24091114 MS.gene44415:intron 30.0%
!!! TTTTTTGTTGTTGCAGGAAA+TGG + chr8.2:24089163-24089182 MS.gene44415:intron 30.0%
AAACTTCCTTGATGCTAATG+AGG + chr8.2:24089220-24089239 MS.gene44415:CDS 35.0%
AATGTGTATTAGTGTCGTGT+TGG - chr8.2:24089850-24089869 None:intergenic 35.0%
CACTTCACACTTATGTTTGA+AGG + chr8.2:24089795-24089814 MS.gene44415:intron 35.0%
CATTAGCATCAAGGAAGTTT+AGG - chr8.2:24089220-24089239 None:intergenic 35.0%
CTGAACTCAAACATTGTTGT+TGG - chr8.2:24089271-24089290 None:intergenic 35.0%
GTCTCAACTCAAAATCCTAA+AGG + chr8.2:24089614-24089633 MS.gene44415:CDS 35.0%
GTTTCTTCTGATACGTTCAT+TGG - chr8.2:24089047-24089066 None:intergenic 35.0%
TAACAAGTTTCTCAGAGTAC+CGG - chr8.2:24090606-24090625 None:intergenic 35.0%
TACTATCATTGCTCAGGTAA+AGG + chr8.2:24090439-24090458 MS.gene44415:intron 35.0%
TATCAGAAGAAACCAGCAAA+TGG + chr8.2:24089054-24089073 MS.gene44415:CDS 35.0%
TCATCAATGATACTATGGTC+TGG - chr8.2:24090197-24090216 None:intergenic 35.0%
TCTTGTTCCTAACCTGAAAA+AGG + chr8.2:24089769-24089788 MS.gene44415:CDS 35.0%
TGACATTACTATCATTGCTC+AGG + chr8.2:24090433-24090452 MS.gene44415:CDS 35.0%
TGTTTCATCAGTGAATGAGT+TGG - chr8.2:24089315-24089334 None:intergenic 35.0%
! AACAATTGTTTTCAGCTCCA+TGG - chr8.2:24089381-24089400 None:intergenic 35.0%
! ATACTTTTGTAGATGGGTGA+AGG + chr8.2:24091389-24091408 MS.gene44415:intron 35.0%
! TACTTTTGTAGATGGGTGAA+GGG + chr8.2:24091390-24091409 MS.gene44415:intron 35.0%
! TTTGACTTTGAGTTTAAGGC+AGG - chr8.2:24089545-24089564 None:intergenic 35.0%
!! AAGTTTAGGTTGCATTCTTC+AGG - chr8.2:24089206-24089225 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG + chr8.2:24090954-24090973 MS.gene44415:intron 35.0%
!! GAAAGAGCTTAAAAAGCGTT+TGG + chr8.2:24090112-24090131 MS.gene44415:CDS 35.0%
!! TGGAGAAAGTTTTGGTGAAA+TGG - chr8.2:24089426-24089445 None:intergenic 35.0%
!! TGTGAAGTGTTACCTTTTTC+AGG - chr8.2:24089784-24089803 None:intergenic 35.0%
!! TTGAGTGCTATTTAGCTAGT+GGG - chr8.2:24089980-24089999 None:intergenic 35.0%
!! TTTGAGTGCTATTTAGCTAG+TGG - chr8.2:24089981-24090000 None:intergenic 35.0%
!!! AGTGTTACCTTTTTCAGGTT+AGG - chr8.2:24089779-24089798 None:intergenic 35.0%
!!! CAACATTGATTTTGAAAGCG+AGG - chr8.2:24090829-24090848 None:intergenic 35.0%
!!! GATGATGATGGAGAAAGTTT+TGG - chr8.2:24089434-24089453 None:intergenic 35.0%
!!! GTTTTTTAACATGTGCCCTA+AGG + chr8.2:24091094-24091113 MS.gene44415:intron 35.0%
!!! TGAGTTTTGGATAACCCTTT+AGG - chr8.2:24089632-24089651 None:intergenic 35.0%
AATAGCAGTGTCTTACCCTT+TGG + chr8.2:24090401-24090420 MS.gene44415:CDS 40.0%
AATGTCAATGGAATCCCCAA+AGG - chr8.2:24090420-24090439 None:intergenic 40.0%
ACACGTATTCATGAAAGGGA+GGG + chr8.2:24091236-24091255 MS.gene44415:intron 40.0%
ACTCTTCCTCATTAGCATCA+AGG - chr8.2:24089229-24089248 None:intergenic 40.0%
AGGGTACAACTTGAGCATAA+AGG + chr8.2:24091409-24091428 MS.gene44415:CDS 40.0%
ATAGCAGTGTCTTACCCTTT+GGG + chr8.2:24090402-24090421 MS.gene44415:CDS 40.0%
ATGTCAATGGAATCCCCAAA+GGG - chr8.2:24090419-24090438 None:intergenic 40.0%
CAAATCAGAAAGCCATTTGC+TGG - chr8.2:24089069-24089088 None:intergenic 40.0%
CAGCAAATGGCTTTCTGATT+TGG + chr8.2:24089067-24089086 MS.gene44415:CDS 40.0%
GCTTTCTTTCAGCCATGATA+TGG - chr8.2:24089709-24089728 None:intergenic 40.0%
TACACGTATTCATGAAAGGG+AGG + chr8.2:24091235-24091254 MS.gene44415:intron 40.0%
TGAAATTGAGAGACCAACCA+TGG + chr8.2:24089361-24089380 MS.gene44415:CDS 40.0%
TGAATGTTTCTCATTTGGGG+TGG - chr8.2:24089593-24089612 None:intergenic 40.0%
TGGTTTCTTCAGAACAACCA+CGG - chr8.2:24090177-24090196 None:intergenic 40.0%
TTACTGTCGATGTTGTGTCT+TGG + chr8.2:24089919-24089938 MS.gene44415:intron 40.0%
TTCAGCCATGATATGGTCAT+CGG - chr8.2:24089702-24089721 None:intergenic 40.0%
! ATTGTTTTCAGCTCCATGGT+TGG - chr8.2:24089377-24089396 None:intergenic 40.0%
! CACTTTTGCTTGCACTTGAA+AGG - chr8.2:24090282-24090301 None:intergenic 40.0%
! TGTTGGTAGGAGGAGAATTT+GGG - chr8.2:24089499-24089518 None:intergenic 40.0%
! TGTTGTTGGGTTTGTTCTTG+TGG - chr8.2:24089257-24089276 None:intergenic 40.0%
!! AAGTGCAAGCAAAAGTGTCA+GGG + chr8.2:24090285-24090304 MS.gene44415:CDS 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG - chr8.2:24091112-24091131 None:intergenic 40.0%
!! GAATGAGTTGGTGATAGTTG+TGG - chr8.2:24089303-24089322 None:intergenic 40.0%
!! GTTGGTGATAGTTGTGGAAT+TGG - chr8.2:24089297-24089316 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG - chr8.2:24091113-24091132 None:intergenic 40.0%
!! TGAGTGCTATTTAGCTAGTG+GGG - chr8.2:24089979-24089998 None:intergenic 40.0%
ACTTGTCAAGAACCTACGCA+TGG + chr8.2:24091433-24091452 MS.gene44415:CDS 45.0%
AGGAGTGAGTCCACAAAACT+GGG - chr8.2:24089525-24089544 None:intergenic 45.0%
CTGCAAGAGCAATGAAGCTT+TGG - chr8.2:24089748-24089767 None:intergenic 45.0%
GAAAACAAAGGTAGAGTCCG+TGG + chr8.2:24090157-24090176 MS.gene44415:CDS 45.0%
TAGCAGTGTCTTACCCTTTG+GGG + chr8.2:24090403-24090422 MS.gene44415:CDS 45.0%
! ATTCACTGCCAGAAGCACAA+GGG + chr8.2:24090326-24090345 MS.gene44415:CDS 45.0%
! CAGGGAAAGAGATGCTGATT+CGG + chr8.2:24090303-24090322 MS.gene44415:CDS 45.0%
! GTGTTGGTAGGAGGAGAATT+TGG - chr8.2:24089500-24089519 None:intergenic 45.0%
!! CAAGTGCAAGCAAAAGTGTC+AGG + chr8.2:24090284-24090303 MS.gene44415:CDS 45.0%
!! GGGAGGGCGTATTTTATTCT+AGG + chr8.2:24091252-24091271 MS.gene44415:intron 45.0%
!! ACTAATTATTATTAATTATT+AGG + chr8.2:24090570-24090589 MS.gene44415:intron 5.0%
!! CTAATTATTATTAATTATTA+GGG + chr8.2:24090571-24090590 MS.gene44415:intron 5.0%
ACTTCAATGCTGCCATGCGT+AGG - chr8.2:24091448-24091467 None:intergenic 50.0%
AGTGAGTCCACAAAACTGGG+GGG - chr8.2:24089522-24089541 None:intergenic 50.0%
CAACGCCGATGACCATATCA+TGG + chr8.2:24089694-24089713 MS.gene44415:CDS 50.0%
CAGGAGTGAGTCCACAAAAC+TGG - chr8.2:24089526-24089545 None:intergenic 50.0%
CATGATATGGTCATCGGCGT+TGG - chr8.2:24089696-24089715 None:intergenic 50.0%
GAGTGAGTCCACAAAACTGG+GGG - chr8.2:24089523-24089542 None:intergenic 50.0%
GGAGTGAGTCCACAAAACTG+GGG - chr8.2:24089524-24089543 None:intergenic 50.0%
GGGGATTCTGGTGAAAGTCA+TGG + chr8.2:24090346-24090365 MS.gene44415:CDS 50.0%
GGTCATCTGAGTGACACGTT+TGG - chr8.2:24090807-24090826 None:intergenic 50.0%
TGGTAGGAGGAGAATTTGGG+AGG - chr8.2:24089496-24089515 None:intergenic 50.0%
! GATTCACTGCCAGAAGCACA+AGG + chr8.2:24090325-24090344 MS.gene44415:CDS 50.0%
! TTCACTGCCAGAAGCACAAG+GGG + chr8.2:24090327-24090346 MS.gene44415:CDS 50.0%
CCAGAATCCCCTTGTGCTTC+TGG - chr8.2:24090337-24090356 None:intergenic 55.0%
! ACCAACACCCCCCAGTTTTG+TGG + chr8.2:24089512-24089531 MS.gene44415:CDS 55.0%
! CCAGAAGCACAAGGGGATTC+TGG + chr8.2:24090334-24090353 MS.gene44415:CDS 55.0%
! TCCACAAAACTGGGGGGTGT+TGG - chr8.2:24089516-24089535 None:intergenic 55.0%
!! CAAAACTGGGGGGTGTTGGT+AGG - chr8.2:24089512-24089531 None:intergenic 55.0%
!! AACTGGGGGGTGTTGGTAGG+AGG - chr8.2:24089509-24089528 None:intergenic 60.0%
!!! GGCGTTGGCTCGAGACCTTT+TGG - chr8.2:24089681-24089700 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 24089023 24091490 24089023 ID=MS.gene44415
chr8.2 mRNA 24089023 24091490 24089023 ID=MS.gene44415.t1;Parent=MS.gene44415
chr8.2 exon 24089023 24089088 24089023 ID=MS.gene44415.t1.exon1;Parent=MS.gene44415.t1
chr8.2 CDS 24089023 24089088 24089023 ID=cds.MS.gene44415.t1;Parent=MS.gene44415.t1
chr8.2 exon 24089179 24089790 24089179 ID=MS.gene44415.t1.exon2;Parent=MS.gene44415.t1
chr8.2 CDS 24089179 24089790 24089179 ID=cds.MS.gene44415.t1;Parent=MS.gene44415.t1
chr8.2 exon 24090071 24090454 24090071 ID=MS.gene44415.t1.exon3;Parent=MS.gene44415.t1
chr8.2 CDS 24090071 24090454 24090071 ID=cds.MS.gene44415.t1;Parent=MS.gene44415.t1
chr8.2 exon 24091401 24091490 24091401 ID=MS.gene44415.t1.exon4;Parent=MS.gene44415.t1
chr8.2 CDS 24091401 24091490 24091401 ID=cds.MS.gene44415.t1;Parent=MS.gene44415.t1
Gene Sequence

>MS.gene44415

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGTAAGAAAATTAAAGGCAAATTAACATATTATTGATTATATAAGTAAATTCTTTATCATTTTAAATTTTAATTTTTTTTTGTTGTTGCAGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCATACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTTATGCTAACCGGTACTCTGAGAAACTTGTTAAGAATAAAAAAAGGAATTTATTACCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTGTCTAGGGGCCAAACGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTGTTTTTTTTTTGGAAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTGTAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAACTTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATACTTCTTCTTTTGGGGTTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTTATATCAGGATGTTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>MS.gene44415.t1

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTFDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSIQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE