AlfalfaGEDB | MS.gene44417

M. sativa cultivar XinJiangDaYe / MS.gene44417

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44417.t1	XP_013442259.1	72.2	180	23	2	1	153	201	380	2.20E-51	211.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44417.t1	Q9T072	42.0	174	77	1	1	150	155	328	3.8e-28	125.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44417.t1	A0A072TFG1	72.2	180	23	2	1	153	201	380	1.6e-51	211.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene44417.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44417.t1	MTR_0246s0020	80.000	180	9	1	1	153	201	380	3.49e-93	275
MS.gene44417.t1	MTR_0246s0020	72.093	172	19	2	1	145	201	370	4.03e-75	228
MS.gene44417.t1	MTR_0011s0260	68.391	174	32	2	1	151	186	359	4.06e-74	226
MS.gene44417.t1	MTR_0011s0210	67.614	176	34	2	1	153	186	361	4.36e-73	223
MS.gene44417.t1	MTR_0250s0040	64.571	175	39	2	1	152	91	265	4.95e-72	217
MS.gene44417.t1	MTR_6g047570	63.429	175	41	2	1	152	162	336	3.96e-70	214
MS.gene44417.t1	MTR_0246s0050	64.286	168	44	3	1	152	154	321	3.94e-68	209
MS.gene44417.t1	MTR_4g067010	64.118	170	41	2	1	150	150	319	5.34e-68	209
MS.gene44417.t1	MTR_4g098035	64.286	168	44	3	1	152	175	342	6.63e-68	209
MS.gene44417.t1	MTR_4g066460	62.209	172	45	3	1	152	158	329	4.52e-65	201

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44417.t1	AT4G37850	43.103	174	75	2	1	150	155	328	2.34e-39	135
MS.gene44417.t1	AT4G37850	43.103	174	75	2	1	150	155	328	2.34e-39	135
MS.gene44417.t1	AT2G22760	42.442	172	72	5	1	145	122	293	2.02e-37	130
MS.gene44417.t1	AT2G22750	42.614	176	74	2	1	149	129	304	2.47e-37	130
MS.gene44417.t1	AT2G22750	42.614	176	74	2	1	149	157	332	4.27e-37	130
MS.gene44417.t1	AT2G22750	42.614	176	74	2	1	149	153	328	4.56e-37	129
MS.gene44417.t1	AT2G22750	42.614	176	73	3	1	149	129	303	6.35e-36	126
MS.gene44417.t1	AT2G22750	42.614	176	73	3	1	149	157	331	1.20e-35	126
MS.gene44417.t1	AT2G22770	34.973	183	81	3	1	145	135	317	2.95e-31	114
MS.gene44417.t1	AT2G22760	41.176	153	64	4	1	127	122	274	2.59e-30	111

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Find 26 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTGCAAGCAAAAGTGTCA+GGG	0.633121	8.1:+24662477	MS.gene44417:intron
AATAGCAGTGTCTTACCCTT+TGG	0.385303	8.1:+24662593	MS.gene44417:CDS
AATGTCAATGGAATCCCCAA+AGG	0.614554	8.1:-24662609	None:intergenic
ACTTCAATGCTGCCATGCGT+AGG	0.601033	8.1:-24663637	None:intergenic
ACTTGTCAAGAACCTACGCA+TGG	0.686710	8.1:+24663625	MS.gene44417:CDS
AGAACAAAACAAGAAAACAA+AGG	0.679620	8.1:+24662340	MS.gene44417:CDS
AGCAATGATAGTAATGTCAA+TGG	0.495292	8.1:-24662621	None:intergenic
AGGGTACAACTTGAGCATAA+AGG	0.600355	8.1:+24663601	MS.gene44417:CDS
ATAGCAGTGTCTTACCCTTT+GGG	0.356671	8.1:+24662594	MS.gene44417:CDS
ATGTCAATGGAATCCCCAAA+GGG	0.596532	8.1:-24662608	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATATCATTAATCAATGCCC+CGG	+	chr8.1:24662923-24662942	MS.gene44417:intron	30.0%
AATTAATGAAATGCTAACCG+GGG	-	chr8.1:24662943-24662962	None:intergenic	30.0%
AGCAATGATAGTAATGTCAA+TGG	-	chr8.1:24662624-24662643	None:intergenic	30.0%
ATTATTAGGGTTATGCTAAC+CGG	+	chr8.1:24662776-24662795	MS.gene44417:intron	30.0%
CATCATCATCAATGATACTA+TGG	-	chr8.1:24662397-24662416	None:intergenic	30.0%
TAAAATTTGGTAGATGGACA+AGG	+	chr8.1:24662253-24662272	MS.gene44417:intron	30.0%
TTAGTGGCAATTATTAAGGT+AGG	-	chr8.1:24662667-24662686	None:intergenic	30.0%
AATGTGTATTAGTGTCGTGT+TGG	-	chr8.1:24662045-24662064	None:intergenic	35.0%
CACTTCACACTTATGTTTGA+AGG	+	chr8.1:24661990-24662009	MS.gene44417:intron	35.0%
TAACAAGTTTCTCAGAGTAC+CGG	-	chr8.1:24662798-24662817	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	24661908	24663682	24661908	ID=MS.gene44417
chr8.1	mRNA	24661908	24663682	24661908	ID=MS.gene44417.t1;Parent=MS.gene44417
chr8.1	exon	24661908	24661985	24661908	ID=MS.gene44417.t1.exon1;Parent=MS.gene44417.t1
chr8.1	CDS	24661908	24661985	24661908	ID=cds.MS.gene44417.t1;Parent=MS.gene44417.t1
chr8.1	exon	24662266	24662398	24662266	ID=MS.gene44417.t1.exon2;Parent=MS.gene44417.t1
chr8.1	CDS	24662266	24662398	24662266	ID=cds.MS.gene44417.t1;Parent=MS.gene44417.t1
chr8.1	exon	24662480	24662646	24662480	ID=MS.gene44417.t1.exon3;Parent=MS.gene44417.t1
chr8.1	CDS	24662480	24662646	24662480	ID=cds.MS.gene44417.t1;Parent=MS.gene44417.t1
chr8.1	exon	24663593	24663682	24663593	ID=MS.gene44417.t1.exon4;Parent=MS.gene44417.t1
chr8.1	CDS	24663593	24663682	24663593	ID=cds.MS.gene44417.t1;Parent=MS.gene44417.t1

Gene Sequence

>MS.gene44417

ATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCATACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTTATGCTAACCGGTACTCTGAGAAACTTGTTAAGAATAAAAAAAGGAATTTATTACCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTGTCTAGGGGCCAAACGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTGTTTTTTTTTTGGAAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTGTAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAACTTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATACTTCTTCTTTTGGGGTTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTTATATCAGGATGTTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA

Protein sequence

>MS.gene44417.t1

MAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHMQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSIQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE