AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44418

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44418.t1 MTR_4g097950 90.789 228 18 2 2 228 26 251 8.67e-129 374
MS.gene44418.t1 MTR_5g014600 53.571 224 94 4 6 225 116 333 8.50e-62 205
MS.gene44418.t1 MTR_5g014520 47.788 226 102 5 2 224 115 327 9.72e-47 165
MS.gene44418.t1 MTR_4g092700 54.749 179 70 4 45 222 158 326 1.10e-46 165
MS.gene44418.t1 MTR_2g010450 51.397 179 77 4 45 222 177 346 1.53e-46 165
MS.gene44418.t1 MTR_0250s0040 44.845 194 96 3 33 225 76 259 4.85e-43 153
MS.gene44418.t1 MTR_0246s0020 43.697 238 111 7 3 224 142 372 5.23e-42 154
MS.gene44418.t1 MTR_4g009540 42.857 217 107 5 15 222 118 326 4.53e-40 147
MS.gene44418.t1 MTR_5g014560 43.655 197 102 3 29 225 115 302 1.84e-39 145
MS.gene44418.t1 MTR_6g047570 44.681 188 93 3 37 223 151 328 8.73e-39 144
MS.gene44418.t1 MTR_0246s0020 43.750 224 103 7 3 210 142 358 1.90e-38 144
MS.gene44418.t1 MTR_4g009540 44.279 201 95 5 15 206 118 310 5.87e-38 141
MS.gene44418.t1 MTR_4g067010 43.617 188 86 3 42 225 144 315 9.87e-37 138
MS.gene44418.t1 MTR_0011s0210 45.251 179 87 2 45 222 183 351 1.40e-36 138
MS.gene44418.t1 MTR_0011s0260 46.369 179 85 3 45 222 183 351 1.50e-36 138
MS.gene44418.t1 MTR_4g097940 44.199 181 82 4 45 221 155 320 1.95e-36 137
MS.gene44418.t1 MTR_4g066460 44.271 192 95 2 34 225 144 323 4.26e-36 136
MS.gene44418.t1 MTR_2g104550 41.256 223 116 6 8 228 104 313 7.43e-36 135
MS.gene44418.t1 MTR_4g009110 37.443 219 129 3 5 222 134 345 9.24e-36 136
MS.gene44418.t1 MTR_0246s0050 40.099 202 102 3 24 224 131 314 2.67e-33 128
MS.gene44418.t1 MTR_4g098035 40.099 202 102 3 24 224 152 335 5.30e-33 128
MS.gene44418.t1 MTR_4g097920 41.573 178 91 3 45 221 140 305 1.54e-32 126
MS.gene44418.t1 MTR_4g097920 41.573 178 91 3 45 221 155 320 1.82e-32 126
MS.gene44418.t1 MTR_8g009020 39.252 214 115 4 20 230 125 326 8.35e-32 124
MS.gene44418.t1 MTR_2g104490 37.788 217 122 4 16 231 108 312 1.40e-31 124
MS.gene44418.t1 MTR_4g066380 43.005 193 95 3 34 225 142 320 1.00e-30 122
MS.gene44418.t1 MTR_1g105765 27.459 244 166 4 237 476 35 271 7.31e-30 118
MS.gene44418.t1 MTR_1g105780 27.049 244 167 4 237 476 35 271 1.08e-29 117
MS.gene44418.t1 MTR_5g014640 36.869 198 113 1 28 225 137 322 1.24e-29 118
MS.gene44418.t1 MTR_2g104560 38.421 190 96 4 46 230 55 228 1.39e-29 115
MS.gene44418.t1 MTR_2g104490 41.969 193 97 6 16 206 108 287 1.63e-29 117
MS.gene44418.t1 MTR_2g104500 34.286 210 123 2 13 222 73 267 5.84e-28 112
MS.gene44418.t1 MTR_1g084880 27.160 243 168 3 237 474 34 272 2.07e-26 108
MS.gene44418.t1 MTR_4g032560 27.572 243 170 2 233 474 59 296 3.27e-26 108
MS.gene44418.t1 MTR_4g032580 27.572 243 170 2 233 474 59 296 5.08e-26 108
MS.gene44418.t1 MTR_3g048970 26.749 243 169 3 237 474 34 272 8.70e-26 106
MS.gene44418.t1 MTR_2g104530 36.517 178 98 2 46 222 106 269 3.25e-25 105
MS.gene44418.t1 MTR_7g080780 34.653 202 114 1 21 222 128 311 3.80e-25 105
MS.gene44418.t1 MTR_7g080780 34.653 202 114 1 21 222 141 324 4.61e-25 105
MS.gene44418.t1 MTR_2g104650 37.688 199 113 4 28 223 75 265 4.92e-24 102
MS.gene44418.t1 MTR_2g104590 36.869 198 96 6 28 223 50 220 5.56e-24 100
MS.gene44418.t1 MTR_3g070250 27.039 233 169 1 237 468 39 271 4.97e-23 99.0
MS.gene44418.t1 MTR_8g069740 37.430 179 101 4 33 208 98 268 6.47e-23 98.6
MS.gene44418.t1 MTR_2g100360 28.111 217 143 4 239 448 36 246 8.38e-23 98.2
MS.gene44418.t1 MTR_8g009010 36.275 204 76 4 45 239 134 292 9.81e-23 99.0
MS.gene44418.t1 MTR_8g064700 31.527 203 129 3 25 227 224 416 2.06e-22 99.8
MS.gene44418.t1 MTR_6g047550 56.322 87 38 0 26 112 154 240 3.59e-22 96.3
MS.gene44418.t1 MTR_5g014550 45.038 131 63 2 75 205 54 175 6.70e-22 93.6
MS.gene44418.t1 MTR_6g465600 26.016 246 154 5 242 474 45 275 1.29e-20 92.8
MS.gene44418.t1 MTR_2g038740 27.143 210 148 2 237 443 34 241 1.28e-19 92.4
MS.gene44418.t1 MTR_1g052720 26.638 229 154 4 240 463 40 259 1.62e-19 89.4
MS.gene44418.t1 MTR_7g110090 24.268 239 175 2 237 474 34 267 1.64e-19 89.0
MS.gene44418.t1 MTR_1g087870 29.146 199 136 2 268 463 13 209 5.03e-19 87.0
MS.gene44418.t1 MTR_5g066660 25.106 235 139 4 239 470 36 236 2.41e-18 85.5
MS.gene44418.t1 MTR_3g085990 30.469 128 84 1 234 361 32 154 6.27e-18 83.6
MS.gene44418.t1 MTR_4g062370 24.658 219 159 2 237 454 38 251 8.03e-18 87.0
MS.gene44418.t1 MTR_4g009510 32.597 181 100 4 44 223 42 201 3.50e-17 80.5
MS.gene44418.t1 MTR_7g451650 33.544 158 86 4 68 225 57 195 4.57e-17 80.1
MS.gene44418.t1 MTR_4g009420 30.588 170 90 3 56 225 54 195 9.86e-17 79.0
MS.gene44418.t1 MTR_7g050350 32.895 152 83 4 74 225 1 133 2.24e-16 76.3
MS.gene44418.t1 MTR_7g451650 32.895 152 83 4 74 225 1 133 2.24e-16 76.3
MS.gene44418.t1 MTR_1g047540 25.114 219 143 3 237 454 34 232 2.34e-16 81.6
MS.gene44418.t1 MTR_0246s0040 25.249 301 191 7 176 474 6 274 2.78e-16 80.1
MS.gene44418.t1 MTR_8g024790 30.481 187 111 6 30 211 276 448 6.15e-15 77.4
MS.gene44418.t1 MTR_8g067280 33.714 175 106 3 21 189 438 608 7.50e-15 77.4
MS.gene44418.t1 MTR_7g117670 31.944 144 81 2 45 188 402 528 4.02e-14 75.1
MS.gene44418.t1 MTR_1g069260 24.291 247 147 4 237 470 61 280 1.34e-13 71.6
MS.gene44418.t1 MTR_8g027495 34.146 123 64 2 45 167 458 563 2.53e-13 72.8
MS.gene44418.t1 MTR_8g024770 31.646 158 98 3 5 162 244 391 3.41e-13 72.0
MS.gene44418.t1 MTR_7g096350 53.571 56 26 0 45 100 355 410 4.17e-13 71.6
MS.gene44418.t1 MTR_5g030430 41.573 89 52 0 10 98 458 546 4.68e-13 72.0
MS.gene44418.t1 MTR_7g451650 34.532 139 72 4 68 206 57 176 5.19e-13 68.2
MS.gene44418.t1 MTR_1g072320 30.000 150 96 4 45 190 470 614 1.06e-12 70.9
MS.gene44418.t1 MTR_4g009420 31.126 151 76 3 56 206 54 176 1.67e-12 67.0
MS.gene44418.t1 MTR_4g117740 21.547 181 103 3 250 425 53 199 2.00e-12 66.6
MS.gene44418.t1 MTR_2g084280 27.907 172 111 3 47 217 135 294 2.63e-12 68.2
MS.gene44418.t1 MTR_3g030700 30.247 162 103 3 47 205 198 352 4.05e-12 68.2
MS.gene44418.t1 MTR_0091s0090 25.773 194 123 4 283 473 2 177 4.94e-12 66.6
MS.gene44418.t1 MTR_3g498695 27.273 198 116 6 13 200 275 454 6.37e-12 67.8
MS.gene44418.t1 MTR_3g498695 27.273 198 116 6 13 200 275 454 6.53e-12 67.8
MS.gene44418.t1 MTR_2g081040 30.435 161 99 4 47 200 179 333 8.91e-12 67.0
MS.gene44418.t1 MTR_0521s0010 23.958 192 124 3 237 424 28 201 2.71e-11 63.9
MS.gene44418.t1 MTR_3g104100 27.950 161 103 3 47 200 197 351 3.79e-11 65.1
MS.gene44418.t1 MTR_2g038040 24.201 219 135 6 27 231 37 238 4.58e-11 63.5
MS.gene44418.t1 MTR_5g075200 24.000 125 90 1 250 374 60 179 5.53e-11 62.4
MS.gene44418.t1 MTR_7g083900 27.500 160 109 3 46 200 287 444 8.06e-11 64.3
MS.gene44418.t1 MTR_7g083900 27.500 160 109 3 46 200 287 444 8.15e-11 64.3
MS.gene44418.t1 MTR_4g057270 28.205 156 104 4 47 202 151 298 8.42e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44418.t1 AT4G37850 47.264 201 97 4 25 225 133 324 9.27e-47 165
MS.gene44418.t1 AT4G37850 47.264 201 97 4 25 225 133 324 9.27e-47 165
MS.gene44418.t1 AT2G22750 48.980 196 94 4 30 225 112 301 8.30e-38 140
MS.gene44418.t1 AT2G22750 47.959 196 96 4 30 225 136 325 9.45e-38 141
MS.gene44418.t1 AT2G22750 47.959 196 96 4 30 225 140 329 1.25e-37 140
MS.gene44418.t1 AT2G22750 48.980 196 93 5 30 225 112 300 2.36e-36 136
MS.gene44418.t1 AT2G22750 47.959 196 95 5 30 225 140 328 2.87e-36 137
MS.gene44418.t1 AT2G22760 40.796 201 104 5 33 225 101 294 4.59e-36 135
MS.gene44418.t1 AT2G22770 38.220 191 112 2 41 225 128 318 1.04e-32 127
MS.gene44418.t1 AT2G22760 40.884 181 93 4 33 206 101 274 8.20e-32 123
MS.gene44418.t1 AT4G29930 26.941 219 137 6 27 231 39 248 2.82e-16 79.0
MS.gene44418.t1 AT2G46510 44.211 95 46 1 23 117 373 460 1.86e-15 79.3
MS.gene44418.t1 AT5G46760 34.437 151 82 4 45 189 415 554 5.11e-15 78.2
MS.gene44418.t1 AT4G29930 28.351 194 119 5 27 206 39 226 5.28e-15 75.5
MS.gene44418.t1 AT4G29930 28.351 194 119 5 27 206 39 226 5.28e-15 75.5
MS.gene44418.t1 AT2G16910 28.090 178 115 3 46 210 315 492 1.52e-14 76.6
MS.gene44418.t1 AT4G29930 25.837 209 123 6 27 231 39 219 2.09e-14 72.8
MS.gene44418.t1 AT4G29930 25.243 206 131 5 27 231 39 222 2.61e-14 72.8
MS.gene44418.t1 AT1G01260 28.834 163 79 3 30 189 418 546 1.20e-13 73.6
MS.gene44418.t1 AT1G01260 28.834 163 79 3 30 189 418 546 1.20e-13 73.6
MS.gene44418.t1 AT1G01260 28.834 163 79 3 30 189 418 546 1.20e-13 73.6
MS.gene44418.t1 AT1G32640 31.875 160 101 2 30 189 437 588 1.83e-13 73.2
MS.gene44418.t1 AT4G29930 27.174 184 105 5 27 206 39 197 3.38e-13 69.7
MS.gene44418.t1 AT5G58010 34.459 148 80 4 43 186 107 241 5.21e-13 70.1
MS.gene44418.t1 AT4G17880 32.468 154 77 3 45 189 416 551 1.07e-12 70.9
MS.gene44418.t1 AT5G57150 30.000 150 101 3 46 192 56 204 2.27e-12 67.8
MS.gene44418.t1 AT5G57150 26.776 183 130 3 46 227 55 234 2.68e-12 67.0
MS.gene44418.t1 AT5G57150 30.667 150 100 3 46 192 55 203 3.23e-12 66.6
MS.gene44418.t1 AT4G16430 37.179 78 49 0 25 102 300 377 4.48e-12 68.6
MS.gene44418.t1 AT5G57150 30.667 150 100 3 46 192 56 204 4.65e-12 66.2
MS.gene44418.t1 AT5G57150 30.667 150 100 3 46 192 56 204 5.04e-12 65.9
MS.gene44418.t1 AT1G12860 28.994 169 107 3 46 209 268 428 7.03e-12 67.8
MS.gene44418.t1 AT1G12860 30.000 160 99 3 46 200 268 419 8.20e-12 67.4
MS.gene44418.t1 AT4G29930 32.174 115 73 2 27 141 39 148 2.07e-11 63.2
MS.gene44418.t1 AT1G63650 26.590 173 117 4 39 202 399 570 5.02e-11 65.5
MS.gene44418.t1 AT1G63650 26.590 173 117 4 39 202 399 570 5.02e-11 65.5
MS.gene44418.t1 AT1G63650 26.590 173 117 4 39 202 399 570 5.02e-11 65.5
MS.gene44418.t1 AT2G28160 26.857 175 93 4 32 192 118 271 5.13e-11 64.3
MS.gene44418.t1 AT2G28160 26.857 175 93 4 32 192 118 271 5.43e-11 64.3
MS.gene44418.t1 AT4G09820 27.536 138 71 2 46 183 364 472 5.46e-11 65.1
MS.gene44418.t1 AT5G41315 27.429 175 112 3 42 202 446 619 5.87e-11 65.5
MS.gene44418.t1 AT5G41315 27.429 175 112 3 42 202 443 616 6.56e-11 65.1
MS.gene44418.t1 AT5G41315 27.429 175 112 3 42 202 438 611 6.94e-11 65.1

Find 107 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCCCTCCTTTACGTTTA+AGG 0.071204 8.1:+24672061 MS.gene44418:CDS
AATACAGACACATTTGATAT+TGG 0.242803 8.1:+24671786 MS.gene44418:intron
GCCACCATGCTCTAATTTAA+AGG 0.254341 8.1:-24671953 None:intergenic
TTCATTTGGTTCCTCTGCTT+TGG 0.281353 8.1:+24669555 MS.gene44418:CDS
TGACAAGAATTGCAAAGAAT+TGG 0.323637 8.1:+24672366 MS.gene44418:CDS
ATATAATTGCTGAGAGAATA+AGG 0.332685 8.1:+24668722 MS.gene44418:CDS
GACAAGGACGAAATCAAGTT+TGG 0.334595 8.1:+24672442 MS.gene44418:CDS
AGGCTAGCACAATTAATTTA+CGG 0.346533 8.1:+24672081 MS.gene44418:CDS
TTTGTATAAGATACCTCGTC+TGG 0.350499 8.1:-24671654 None:intergenic
CCTTATTCCAAACCTCAAAA+AGG 0.354140 8.1:+24668783 MS.gene44418:CDS
TCCTGATTCTTTACACGTCT+TGG 0.356377 8.1:-24668643 None:intergenic
GATACTTGATTTCATAGTTA+TGG 0.358339 8.1:-24672032 None:intergenic
ATACAGACACATTTGATATT+GGG 0.363411 8.1:+24671787 MS.gene44418:intron
CGGGGAAGAATGTGCTGATT+AGG 0.377824 8.1:+24669443 MS.gene44418:CDS
AGATAGACAATGAGTTGAAT+AGG 0.378036 8.1:+24671827 MS.gene44418:CDS
ATCTGCAAGTGTTAACCATT+TGG 0.378713 8.1:-24672187 None:intergenic
TGTACGAGTGGAAGGCATTA+AGG 0.384262 8.1:+24672500 MS.gene44418:CDS
GAACATGAGCATCCAGAAAC+TGG 0.387862 8.1:+24670462 MS.gene44418:CDS
GAAGTTAAAGCTAGTTTGTC+GGG 0.404928 8.1:+24669424 MS.gene44418:CDS
AATTTCTTCACAACCGTAAC+CGG 0.405974 8.1:-24669304 None:intergenic
AAGGAGCGGGGTAGTCGATC+TGG 0.413387 8.1:-24672128 None:intergenic
AATAGAGAAACTTGGTCTCT+TGG 0.416449 8.1:+24669513 MS.gene44418:CDS
GTGCAAAATAGGAATGCATT+GGG 0.424575 8.1:-24672544 None:intergenic
ACAGATCCAGGTGCTGCATT+AGG 0.428950 8.1:-24672105 None:intergenic
AAGGTTGGTACAACCTTCAT+TGG 0.432513 8.1:-24668583 None:intergenic
CTCTCAGCAATTATATGATC+TGG 0.438168 8.1:-24668715 None:intergenic
ATTCTTTGAGTTCACAACTT+TGG 0.438205 8.1:-24668617 None:intergenic
GACAAACTAGCTTTAACTTC+AGG 0.442233 8.1:-24669421 None:intergenic
GTCTGCGGATGTCAATGTTT+CGG 0.442533 8.1:+24669336 MS.gene44418:CDS
CAGTCGCATATGTGGGGATA+AGG 0.445648 8.1:-24672147 None:intergenic
CAAAAGCAAAAGTGTAGAAC+CGG 0.446629 8.1:+24669285 MS.gene44418:CDS
ATTTAAAACAGTCGCATATG+TGG 0.449053 8.1:-24672155 None:intergenic
TTGACAATAGCGGACATCAT+TGG 0.449537 8.1:+24672395 MS.gene44418:CDS
TTAAATTAGAGCATGGTGGC+TGG 0.454237 8.1:+24671956 MS.gene44418:CDS
ACCAAGACGTGTAAAGAATC+AGG 0.456019 8.1:+24668642 MS.gene44418:CDS
ATGGACAAGGCCTCAGTGTT+GGG 0.461686 8.1:+24669202 MS.gene44418:CDS
GTTTCCATTACATGAATTTG+AGG 0.464190 8.1:-24669365 None:intergenic
AGATGTAACATCTGATCAAC+TGG 0.467401 8.1:+24672206 MS.gene44418:CDS
AAAGTATGTGAAGGAGCTTA+AGG 0.470002 8.1:+24669234 MS.gene44418:CDS
CAAAAGTGTAGAACCGGTTA+CGG 0.471591 8.1:+24669291 MS.gene44418:CDS
ACTTCCTCAAATTCATGTAA+TGG 0.472899 8.1:+24669361 MS.gene44418:CDS
GGCCATAACCATCATTGCTC+AGG 0.477071 8.1:+24669576 MS.gene44418:CDS
AAATCAAGTTTGGAGGGGAT+TGG 0.478344 8.1:+24672452 MS.gene44418:CDS
TGTGTGATGAGTTGTAGATT+TGG 0.488926 8.1:-24668692 None:intergenic
AGCAGAGGAACCAAATGAAA+AGG 0.489144 8.1:-24669551 None:intergenic
AAAATGTACCTGAGCAATGA+TGG 0.489397 8.1:-24669584 None:intergenic
TAGTCGATCTGGACAGATCC+AGG 0.489641 8.1:-24672117 None:intergenic
GGAACCAAATGAAAAGGAAC+TGG 0.490603 8.1:-24669545 None:intergenic
AAAGTTGTGAACTCAAAGAA+TGG 0.494937 8.1:+24668619 MS.gene44418:CDS
TCCAGGACTCAATCAATTTG+AGG 0.495516 8.1:+24671867 MS.gene44418:CDS
GGAAAGCTGTATTTCTCTCA+TGG 0.500298 8.1:+24671910 MS.gene44418:CDS
TTAAATTTAGTGTCACCAAA+TGG 0.501672 8.1:+24672172 MS.gene44418:CDS
TTCAACAGTTGCATAAATGC+AGG 0.507573 8.1:-24671597 None:intergenic
TGCAACTGTTGAAGCTAAAG+TGG 0.509493 8.1:+24671607 MS.gene44418:CDS
CGTAAAGGAGGGAGATAACT+CGG 0.517430 8.1:-24672054 None:intergenic
TATGTCACTTTAATCGATCC+AGG 0.521051 8.1:+24671850 MS.gene44418:CDS
TGAGAAAGATAAGGCAGTGA+TGG 0.525707 8.1:+24669474 MS.gene44418:CDS
TGTGCAAAATAGGAATGCAT+TGG 0.525777 8.1:-24672545 None:intergenic
ACCTCAAATTGATTGAGTCC+TGG 0.525940 8.1:-24671868 None:intergenic
TAAGGAACAAGTAAAACTGT+TGG 0.530262 8.1:+24669252 MS.gene44418:CDS
CTGAAAGAGCTATGAACTGT+TGG 0.532934 8.1:-24668759 None:intergenic
TGAAGTTAAAGCTAGTTTGT+CGG 0.533689 8.1:+24669423 MS.gene44418:CDS
TTTAAAACAGTCGCATATGT+GGG 0.537260 8.1:-24672154 None:intergenic
GAGAAAGATAAGGCAGTGAT+GGG 0.538793 8.1:+24669475 MS.gene44418:CDS
GGAACAAGTAAAACTGTTGG+AGG 0.550452 8.1:+24669255 MS.gene44418:CDS
GTTGTCGTGGATAAATATGA+TGG 0.551953 8.1:+24671889 MS.gene44418:CDS
AGGACGAAATCAAGTTTGGA+GGG 0.554190 8.1:+24672446 MS.gene44418:CDS
TACGGTCCTAATGCAGCACC+TGG 0.556503 8.1:+24672099 MS.gene44418:CDS
CCACTCGTACAGATTTCTTG+TGG 0.562884 8.1:-24672488 None:intergenic
TGTGCTAGCCTTAAACGTAA+AGG 0.563280 8.1:-24672069 None:intergenic
AAGAAATCTGTACGAGTGGA+AGG 0.564490 8.1:+24672492 MS.gene44418:CDS
GAGTGGAAGGCATTAAGGCT+TGG 0.567434 8.1:+24672505 MS.gene44418:CDS
TTGTATAAGATACCTCGTCT+GGG 0.567583 8.1:-24671653 None:intergenic
AAGGACGAAATCAAGTTTGG+AGG 0.568349 8.1:+24672445 MS.gene44418:CDS
TATAGAGAAATAGAGAAACT+TGG 0.574558 8.1:+24669505 MS.gene44418:CDS
CAATCAATTTGAGGTTGTCG+TGG 0.576079 8.1:+24671876 MS.gene44418:CDS
CATATGTGGGGATAAGGAGC+GGG 0.578422 8.1:-24672141 None:intergenic
GCTATCAAATACTTTAGCAT+AGG 0.578440 8.1:-24672338 None:intergenic
TACCTGAGCAATGATGGTTA+TGG 0.585304 8.1:-24669578 None:intergenic
GATGGACAAGGCCTCAGTGT+TGG 0.588121 8.1:+24669201 MS.gene44418:intron
GATCCTCTGTGAGAAAGATA+AGG 0.589750 8.1:+24669465 MS.gene44418:CDS
TGCAAAATAGGAATGCATTG+GGG 0.592222 8.1:-24672543 None:intergenic
GGACGAAATCAAGTTTGGAG+GGG 0.613445 8.1:+24672447 MS.gene44418:CDS
CCACAAGAAATCTGTACGAG+TGG 0.616911 8.1:+24672488 MS.gene44418:CDS
GCTAGCCTTAAACGTAAAGG+AGG 0.620035 8.1:-24672066 None:intergenic
AAGGCCTTTAAATTAGAGCA+TGG 0.622444 8.1:+24671949 MS.gene44418:CDS
GATGGTTATGGCCAAAGCAG+AGG 0.622453 8.1:-24669566 None:intergenic
TGGAATAGAAGAACTGAACA+AGG 0.624967 8.1:+24671930 MS.gene44418:CDS
GCAAAATAGGAATGCATTGG+GGG 0.626883 8.1:-24672542 None:intergenic
CAGGAGAGCAACAAGAAGAA+CGG 0.634139 8.1:+24668661 MS.gene44418:CDS
TTGGCAATTATTGACAATAG+CGG 0.636816 8.1:+24672385 MS.gene44418:CDS
ACATCATTGGTTGTTCAATG+TGG 0.637756 8.1:+24672408 MS.gene44418:CDS
GCATATGTGGGGATAAGGAG+CGG 0.639162 8.1:-24672142 None:intergenic
AAGTTAAAGCTAGTTTGTCG+GGG 0.643559 8.1:+24669425 MS.gene44418:CDS
TTAAAACAGTCGCATATGTG+GGG 0.654118 8.1:-24672153 None:intergenic
GAAATTGTCTGAACTGTCTG+CGG 0.654800 8.1:+24669321 MS.gene44418:CDS
CTAGCCTTAAACGTAAAGGA+GGG 0.662726 8.1:-24672065 None:intergenic
TGAAGGTTGTACCAACCTTG+AGG 0.663007 8.1:+24668587 MS.gene44418:CDS
CATATGTTTGTAGATGGACA+AGG 0.664887 8.1:+24669189 MS.gene44418:intron
AGAAAGATAAGGCAGTGATG+GGG 0.666003 8.1:+24669476 MS.gene44418:CDS
CTGCCTTATCTTTCTCACAG+AGG 0.691029 8.1:-24669468 None:intergenic
GCCTTTAAATTAGAGCATGG+TGG 0.693123 8.1:+24671952 MS.gene44418:CDS
TGATGCTATAAAGTATGTGA+AGG 0.702262 8.1:+24669225 MS.gene44418:CDS
TATAAGATACCTCGTCTGGG+TGG 0.727121 8.1:-24671650 None:intergenic
ATATGTGGGGATAAGGAGCG+GGG 0.728179 8.1:-24672140 None:intergenic
TGTGCAGTACCACCCAGACG+AGG 0.739111 8.1:+24671641 MS.gene44418:CDS
TATAGCATCACCCAACACTG+AGG 0.742341 8.1:-24669212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATTTCATATTAAAAAA+TGG + chr8.1:24670238-24670257 MS.gene44418:intron 10.0%
!! AATAAAAATTGCATTAAGAT+TGG - chr8.1:24669820-24669839 None:intergenic 15.0%
!! ATTGTTAAAAAAATGGTATA+AGG - chr8.1:24670070-24670089 None:intergenic 15.0%
!!! ATTTTTAATTAAGGACTATA+TGG + chr8.1:24669602-24669621 MS.gene44418:intron 15.0%
!! AAAAATCAAATGTGCAAAAT+AGG - chr8.1:24672558-24672577 None:intergenic 20.0%
!! AAGTTCAAAGTAATATTACT+CGG + chr8.1:24669985-24670004 MS.gene44418:intron 20.0%
!! ACAACACATTGTTAAAAAAA+TGG - chr8.1:24670077-24670096 None:intergenic 20.0%
!! GTATTTAATGCAAATAAAGT+CGG + chr8.1:24670953-24670972 MS.gene44418:intron 20.0%
!!! AAAAAGTATTTCGACTAAAT+AGG + chr8.1:24671166-24671185 MS.gene44418:intron 20.0%
!!! AAGTCTAGCTTTTATAATAA+AGG - chr8.1:24670745-24670764 None:intergenic 20.0%
!!! AATATTTCTATAAAATAGGC+TGG + chr8.1:24671199-24671218 MS.gene44418:intron 20.0%
!!! ACAGGATCATATTTTTTTTA+AGG - chr8.1:24671359-24671378 None:intergenic 20.0%
!!! ATTTTTTTAACAATGTGTTG+TGG + chr8.1:24670076-24670095 MS.gene44418:intron 20.0%
!!! TTTTAATTTTCGCAATGAAT+GGG + chr8.1:24671233-24671252 MS.gene44418:intron 20.0%
! AAATATGCACCAAAGTTATA+AGG - chr8.1:24671325-24671344 None:intergenic 25.0%
! AATACAGACACATTTGATAT+TGG + chr8.1:24671786-24671805 MS.gene44418:intron 25.0%
! AATATCAAATGTGTCTGTAT+TGG - chr8.1:24671787-24671806 None:intergenic 25.0%
! AGAGGAAGTAATAAAATATG+AGG - chr8.1:24670661-24670680 None:intergenic 25.0%
! ATACAGACACATTTGATATT+GGG + chr8.1:24671787-24671806 MS.gene44418:intron 25.0%
! ATATAATTGCTGAGAGAATA+AGG + chr8.1:24668722-24668741 MS.gene44418:CDS 25.0%
! ATTTGTAGACTTTGAATCAA+CGG - chr8.1:24670897-24670916 None:intergenic 25.0%
! CAACCATATAAAACTCAAAT+AGG - chr8.1:24670700-24670719 None:intergenic 25.0%
! GATACTTGATTTCATAGTTA+TGG - chr8.1:24672035-24672054 None:intergenic 25.0%
! GTATCTTATACAAAAAATCC+TGG + chr8.1:24671663-24671682 MS.gene44418:intron 25.0%
! GTCTAACATCATATAATTAC+AGG - chr8.1:24671377-24671396 None:intergenic 25.0%
! TATAGAGAAATAGAGAAACT+TGG + chr8.1:24669505-24669524 MS.gene44418:CDS 25.0%
! TTAAACATCACAAAAGTTAG+AGG + chr8.1:24670263-24670282 MS.gene44418:intron 25.0%
! TTAAATTTAGTGTCACCAAA+TGG + chr8.1:24672172-24672191 MS.gene44418:CDS 25.0%
! TTAGTCATTAGTACATGTTT+AGG - chr8.1:24670830-24670849 None:intergenic 25.0%
!! TTATAAAAGCTAGACTTGTT+TGG + chr8.1:24670748-24670767 MS.gene44418:intron 25.0%
!! TTGAAAAATGAACCTTTTTG+AGG - chr8.1:24668798-24668817 None:intergenic 25.0%
!!! CTCAGGTACATTTTTAATTA+AGG + chr8.1:24669593-24669612 MS.gene44418:intron 25.0%
!!! CTTTTAATTTTCGCAATGAA+TGG + chr8.1:24671232-24671251 MS.gene44418:intron 25.0%
!!! TGAAAAAGTTTTAGTTTGCA+AGG + chr8.1:24670153-24670172 MS.gene44418:intron 25.0%
!!! TGTCCTATTTGAGTTTTATA+TGG + chr8.1:24670694-24670713 MS.gene44418:intron 25.0%
!!! TTTTTATTGATGTGTTGACT+AGG + chr8.1:24669832-24669851 MS.gene44418:intron 25.0%
AAAGTTGTGAACTCAAAGAA+TGG + chr8.1:24668619-24668638 MS.gene44418:CDS 30.0%
AAATACGCAAACATCATGAA+TGG - chr8.1:24669719-24669738 None:intergenic 30.0%
AATGTAGTGTCAAATTGACT+TGG + chr8.1:24669955-24669974 MS.gene44418:intron 30.0%
AATTGTGTCTTTATGGTGTT+TGG + chr8.1:24670422-24670441 MS.gene44418:intron 30.0%
ACACATGATTGATTGTGATT+TGG + chr8.1:24669072-24669091 MS.gene44418:intron 30.0%
ACACTCTTAAGACATTATGT+TGG - chr8.1:24669927-24669946 None:intergenic 30.0%
ACTTCCTCAAATTCATGTAA+TGG + chr8.1:24669361-24669380 MS.gene44418:CDS 30.0%
AGAGTAATATCTCATCACTA+TGG + chr8.1:24670381-24670400 MS.gene44418:intron 30.0%
AGGCTAGCACAATTAATTTA+CGG + chr8.1:24672081-24672100 MS.gene44418:CDS 30.0%
ATGGAAATCAATTTCTTAGC+GGG + chr8.1:24669869-24669888 MS.gene44418:intron 30.0%
ATTGACCATGATACCATTAT+TGG - chr8.1:24670034-24670053 None:intergenic 30.0%
CATATACTTAGATGAGTGAA+TGG + chr8.1:24670180-24670199 MS.gene44418:intron 30.0%
CTAACTAAATGCTGATCAAA+AGG + chr8.1:24670987-24671006 MS.gene44418:intron 30.0%
GATCGATGTATTAATGCTTT+GGG + chr8.1:24670296-24670315 MS.gene44418:intron 30.0%
GATGGAAATCAATTTCTTAG+CGG + chr8.1:24669868-24669887 MS.gene44418:intron 30.0%
GATTGATTGCGATTTATTGA+CGG + chr8.1:24669132-24669151 MS.gene44418:intron 30.0%
GCTATCAAATACTTTAGCAT+AGG - chr8.1:24672341-24672360 None:intergenic 30.0%
GTTTCCATTACATGAATTTG+AGG - chr8.1:24669368-24669387 None:intergenic 30.0%
TAAGGAACAAGTAAAACTGT+TGG + chr8.1:24669252-24669271 MS.gene44418:CDS 30.0%
TACGACAGCAAAATATATCA+TGG + chr8.1:24671399-24671418 MS.gene44418:intron 30.0%
TCATTAGTACATGTTTAGGA+GGG - chr8.1:24670826-24670845 None:intergenic 30.0%
TCTCGTTGATGTTGTATTAA+CGG + chr8.1:24670111-24670130 MS.gene44418:intron 30.0%
TGACAAGAATTGCAAAGAAT+TGG + chr8.1:24672366-24672385 MS.gene44418:CDS 30.0%
TGATGCTATAAAGTATGTGA+AGG + chr8.1:24669225-24669244 MS.gene44418:CDS 30.0%
TGTCATAAAATGTGTTTAGC+AGG + chr8.1:24669659-24669678 MS.gene44418:intron 30.0%
TTGACGTAATTGTGTCTTTA+TGG + chr8.1:24670415-24670434 MS.gene44418:intron 30.0%
TTGGCAATTATTGACAATAG+CGG + chr8.1:24672385-24672404 MS.gene44418:CDS 30.0%
TTGTCTTCTCCTTATAACTT+TGG + chr8.1:24671313-24671332 MS.gene44418:intron 30.0%
TTTGATCAGCATTTAGTTAG+TGG - chr8.1:24670987-24671006 None:intergenic 30.0%
TTTGTAGACTTTGAATCAAC+GGG - chr8.1:24670896-24670915 None:intergenic 30.0%
! AGAGAAGTCGAAATACTTTT+TGG - chr8.1:24671138-24671157 None:intergenic 30.0%
! AGATAGACAATGAGTTGAAT+AGG + chr8.1:24671827-24671846 MS.gene44418:CDS 30.0%
! ATTCTTTGAGTTCACAACTT+TGG - chr8.1:24668620-24668639 None:intergenic 30.0%
! ATTTAAAACAGTCGCATATG+TGG - chr8.1:24672158-24672177 None:intergenic 30.0%
! TGAAGTTAAAGCTAGTTTGT+CGG + chr8.1:24669423-24669442 MS.gene44418:CDS 30.0%
! TGTATTAATGCTTTGGGTAA+TGG + chr8.1:24670302-24670321 MS.gene44418:intron 30.0%
! TTTAAAACAGTCGCATATGT+GGG - chr8.1:24672157-24672176 None:intergenic 30.0%
! TTTTCGTAAGGATCCAATAA+TGG + chr8.1:24670018-24670037 MS.gene44418:intron 30.0%
!! GACTCGAACTATATTTTTGT+GGG + chr8.1:24668831-24668850 MS.gene44418:intron 30.0%
!! TGACTCGAACTATATTTTTG+TGG + chr8.1:24668830-24668849 MS.gene44418:intron 30.0%
!! TGATATATTTTGCTGTCGTA+AGG - chr8.1:24671399-24671418 None:intergenic 30.0%
!!! AAATGAACCTTTTTGAGGTT+TGG - chr8.1:24668793-24668812 None:intergenic 30.0%
!!! CTGTTTTTAATTAGCATGCA+AGG - chr8.1:24669900-24669919 None:intergenic 30.0%
!!! CTTTTTGAGGTTTGGAATAA+GGG - chr8.1:24668785-24668804 None:intergenic 30.0%
!!! GGTGTTTTTAGTTTTTCGTA+AGG + chr8.1:24670006-24670025 MS.gene44418:intron 30.0%
AAAAAAACTGAGAGTTGGAC+TGG + chr8.1:24670486-24670505 MS.gene44418:CDS 35.0%
AAAATGTACCTGAGCAATGA+TGG - chr8.1:24669587-24669606 None:intergenic 35.0%
AAAATGTGTTTAGCAGGAGA+GGG + chr8.1:24669665-24669684 MS.gene44418:intron 35.0%
AAAGTATGTGAAGGAGCTTA+AGG + chr8.1:24669234-24669253 MS.gene44418:CDS 35.0%
AACAATGTGTTGTGGTCAAT+TGG + chr8.1:24670084-24670103 MS.gene44418:intron 35.0%
AAGGCCTTTAAATTAGAGCA+TGG + chr8.1:24671949-24671968 MS.gene44418:CDS 35.0%
AATAGAGAAACTTGGTCTCT+TGG + chr8.1:24669513-24669532 MS.gene44418:CDS 35.0%
AATTTCTTCACAACCGTAAC+CGG - chr8.1:24669307-24669326 None:intergenic 35.0%
AGATGTAACATCTGATCAAC+TGG + chr8.1:24672206-24672225 MS.gene44418:CDS 35.0%
AGGATCCAATAATGGTATCA+TGG + chr8.1:24670026-24670045 MS.gene44418:intron 35.0%
AGTGAAATATACCGTAGCTA+TGG + chr8.1:24671507-24671526 MS.gene44418:intron 35.0%
ATCTGCAAGTGTTAACCATT+TGG - chr8.1:24672190-24672209 None:intergenic 35.0%
CATATGTTTGTAGATGGACA+AGG + chr8.1:24669189-24669208 MS.gene44418:intron 35.0%
CCTTATTCCAAACCTCAAAA+AGG + chr8.1:24668783-24668802 MS.gene44418:CDS 35.0%
CGATCGATGTATTAATGCTT+TGG + chr8.1:24670295-24670314 MS.gene44418:intron 35.0%
CTCTCAGCAATTATATGATC+TGG - chr8.1:24668718-24668737 None:intergenic 35.0%
CTTGTCCATCTACAAACATA+TGG - chr8.1:24669191-24669210 None:intergenic 35.0%
GGACACATATTATGAGATAG+AGG - chr8.1:24670679-24670698 None:intergenic 35.0%
GTACTCCATATGTTTGTAGA+TGG + chr8.1:24669183-24669202 MS.gene44418:intron 35.0%
GTCATTAGTACATGTTTAGG+AGG - chr8.1:24670827-24670846 None:intergenic 35.0%
GTGATCAAGTGTGAAAATAG+AGG - chr8.1:24670872-24670891 None:intergenic 35.0%
GTGCAAAATAGGAATGCATT+GGG - chr8.1:24672547-24672566 None:intergenic 35.0%
GTGTGAAAATAGAGGTTGAA+CGG - chr8.1:24670864-24670883 None:intergenic 35.0%
GTTGTCGTGGATAAATATGA+TGG + chr8.1:24671889-24671908 MS.gene44418:CDS 35.0%
TAAAATGTGTTTAGCAGGAG+AGG + chr8.1:24669664-24669683 MS.gene44418:intron 35.0%
TAACAGTATGATTGAGTTCC+AGG - chr8.1:24671684-24671703 None:intergenic 35.0%
TCATGATGTTTGCGTATTTG+TGG + chr8.1:24669720-24669739 MS.gene44418:intron 35.0%
TGCAAAATAGGAATGCATTG+GGG - chr8.1:24672546-24672565 None:intergenic 35.0%
TGGAATAGAAGAACTGAACA+AGG + chr8.1:24671930-24671949 MS.gene44418:CDS 35.0%
TGTGCAAAATAGGAATGCAT+TGG - chr8.1:24672548-24672567 None:intergenic 35.0%
TGTGTGATGAGTTGTAGATT+TGG - chr8.1:24668695-24668714 None:intergenic 35.0%
TTAAAACAGTCGCATATGTG+GGG - chr8.1:24672156-24672175 None:intergenic 35.0%
TTCAACAGTTGCATAAATGC+AGG - chr8.1:24671600-24671619 None:intergenic 35.0%
TTGTATAAGATACCTCGTCT+GGG - chr8.1:24671656-24671675 None:intergenic 35.0%
TTTAGGAGGGAAAAATGCTA+GGG - chr8.1:24670813-24670832 None:intergenic 35.0%
TTTGTATAAGATACCTCGTC+TGG - chr8.1:24671657-24671676 None:intergenic 35.0%
! AAGTTAAAGCTAGTTTGTCG+GGG + chr8.1:24669425-24669444 MS.gene44418:CDS 35.0%
! AATGCCAGTTCCTTTTCATT+TGG + chr8.1:24669541-24669560 MS.gene44418:CDS 35.0%
! AATGCTTTGGGTAATGGTAA+TGG + chr8.1:24670308-24670327 MS.gene44418:intron 35.0%
! ACATCATTGGTTGTTCAATG+TGG + chr8.1:24672408-24672427 MS.gene44418:CDS 35.0%
! CAAAAGCAAAAGTGTAGAAC+CGG + chr8.1:24669285-24669304 MS.gene44418:CDS 35.0%
! GAAGTTAAAGCTAGTTTGTC+GGG + chr8.1:24669424-24669443 MS.gene44418:CDS 35.0%
! TACTACCAATAGAAGCACAA+CGG + chr8.1:24671259-24671278 MS.gene44418:intron 35.0%
! TCCCTCAACTTTTTGTGATT+CGG + chr8.1:24671998-24672017 MS.gene44418:CDS 35.0%
! TGTGGTCATTCTTTTTGACA+AGG + chr8.1:24672426-24672445 MS.gene44418:CDS 35.0%
! TTATTGCTGTTCCATAGCTA+CGG - chr8.1:24671521-24671540 None:intergenic 35.0%
!! GACAAACTAGCTTTAACTTC+AGG - chr8.1:24669424-24669443 None:intergenic 35.0%
!! TATGTCACTTTAATCGATCC+AGG + chr8.1:24671850-24671869 MS.gene44418:CDS 35.0%
!! TCGAAATACTTTTTGGACAG+TGG - chr8.1:24671131-24671150 None:intergenic 35.0%
!!! AACTTTGGTTTTTGACCTCA+AGG - chr8.1:24668605-24668624 None:intergenic 35.0%
!!! CCTTTTTGAGGTTTGGAATA+AGG - chr8.1:24668786-24668805 None:intergenic 35.0%
!!! TCAGTTTTTTTGCCAGTTTC+TGG - chr8.1:24670477-24670496 None:intergenic 35.0%
AAATCAAGTTTGGAGGGGAT+TGG + chr8.1:24672452-24672471 MS.gene44418:CDS 40.0%
AAGAAATCTGTACGAGTGGA+AGG + chr8.1:24672492-24672511 MS.gene44418:CDS 40.0%
AAGGACGAAATCAAGTTTGG+AGG + chr8.1:24672445-24672464 MS.gene44418:CDS 40.0%
AAGGCTTGGAGCAAAAACTT+TGG + chr8.1:24672519-24672538 MS.gene44418:CDS 40.0%
AATGACCGTTGTGCTTCTAT+TGG - chr8.1:24671267-24671286 None:intergenic 40.0%
ACCAAGACGTGTAAAGAATC+AGG + chr8.1:24668642-24668661 MS.gene44418:CDS 40.0%
ACCCGAATCACAAAAAGTTG+AGG - chr8.1:24672003-24672022 None:intergenic 40.0%
ACCTCAAATTGATTGAGTCC+TGG - chr8.1:24671871-24671890 None:intergenic 40.0%
AGAAAGATAAGGCAGTGATG+GGG + chr8.1:24669476-24669495 MS.gene44418:CDS 40.0%
AGCAGAGGAACCAAATGAAA+AGG - chr8.1:24669554-24669573 None:intergenic 40.0%
AGGACGAAATCAAGTTTGGA+GGG + chr8.1:24672446-24672465 MS.gene44418:CDS 40.0%
AGGGAAAAATGCTAGGGATT+AGG - chr8.1:24670807-24670826 None:intergenic 40.0%
ATCTCCCTCCTTTACGTTTA+AGG + chr8.1:24672061-24672080 MS.gene44418:CDS 40.0%
ATGAATGGATGCAATCCAAC+AGG - chr8.1:24669704-24669723 None:intergenic 40.0%
CCCGAATCACAAAAAGTTGA+GGG - chr8.1:24672002-24672021 None:intergenic 40.0%
CTAGGAATACAAGTGAGAGA+TGG + chr8.1:24669850-24669869 MS.gene44418:intron 40.0%
CTGAAAGAGCTATGAACTGT+TGG - chr8.1:24668762-24668781 None:intergenic 40.0%
CTGGCAAAAAAACTGAGAGT+TGG + chr8.1:24670481-24670500 MS.gene44418:CDS 40.0%
CTTCGCAAATGTTGCACTAT+GGG + chr8.1:24671557-24671576 MS.gene44418:intron 40.0%
GAAATCAATTTCTTAGCGGG+AGG + chr8.1:24669872-24669891 MS.gene44418:intron 40.0%
GAAATTGTCTGAACTGTCTG+CGG + chr8.1:24669321-24669340 MS.gene44418:CDS 40.0%
GACAAGGACGAAATCAAGTT+TGG + chr8.1:24672442-24672461 MS.gene44418:CDS 40.0%
GAGAAAGATAAGGCAGTGAT+GGG + chr8.1:24669475-24669494 MS.gene44418:CDS 40.0%
GATCCTCTGTGAGAAAGATA+AGG + chr8.1:24669465-24669484 MS.gene44418:CDS 40.0%
GCAAAATAGGAATGCATTGG+GGG - chr8.1:24672545-24672564 None:intergenic 40.0%
GCCACCATGCTCTAATTTAA+AGG - chr8.1:24671956-24671975 None:intergenic 40.0%
GCCTTTAAATTAGAGCATGG+TGG + chr8.1:24671952-24671971 MS.gene44418:CDS 40.0%
GGAAAGCTGTATTTCTCTCA+TGG + chr8.1:24671910-24671929 MS.gene44418:CDS 40.0%
GGAACAAGTAAAACTGTTGG+AGG + chr8.1:24669255-24669274 MS.gene44418:CDS 40.0%
GGAACCAAATGAAAAGGAAC+TGG - chr8.1:24669548-24669567 None:intergenic 40.0%
GGGAAAAATGCTAGGGATTA+GGG - chr8.1:24670806-24670825 None:intergenic 40.0%
GTTTAGGAGGGAAAAATGCT+AGG - chr8.1:24670814-24670833 None:intergenic 40.0%
TACCTGAGCAATGATGGTTA+TGG - chr8.1:24669581-24669600 None:intergenic 40.0%
TAGATTGTGCGTCAGTCAAT+CGG + chr8.1:24668948-24668967 MS.gene44418:intron 40.0%
TCCAGGACTCAATCAATTTG+AGG + chr8.1:24671867-24671886 MS.gene44418:CDS 40.0%
TCCTGATTCTTTACACGTCT+TGG - chr8.1:24668646-24668665 None:intergenic 40.0%
TCTTTATGGTGTTTGGCAGA+TGG + chr8.1:24670429-24670448 MS.gene44418:intron 40.0%
TGAGAAAGATAAGGCAGTGA+TGG + chr8.1:24669474-24669493 MS.gene44418:CDS 40.0%
TGCAACTGTTGAAGCTAAAG+TGG + chr8.1:24671607-24671626 MS.gene44418:CDS 40.0%
TTAAATTAGAGCATGGTGGC+TGG + chr8.1:24671956-24671975 MS.gene44418:CDS 40.0%
! CAAAAGTGTAGAACCGGTTA+CGG + chr8.1:24669291-24669310 MS.gene44418:CDS 40.0%
! CAATCAATTTGAGGTTGTCG+TGG + chr8.1:24671876-24671895 MS.gene44418:CDS 40.0%
! CCCTCAACTTTTTGTGATTC+GGG + chr8.1:24671999-24672018 MS.gene44418:CDS 40.0%
! GGCTATAAATGCGCATTGAT+CGG - chr8.1:24670640-24670659 None:intergenic 40.0%
! GTACACGCAGTAAACTTGTT+TGG + chr8.1:24670590-24670609 MS.gene44418:intron 40.0%
! TTCATTTGGTTCCTCTGCTT+TGG + chr8.1:24669555-24669574 MS.gene44418:CDS 40.0%
!! CTAGCCTTAAACGTAAAGGA+GGG - chr8.1:24672068-24672087 None:intergenic 40.0%
!! TGTGCTAGCCTTAAACGTAA+AGG - chr8.1:24672072-24672091 None:intergenic 40.0%
!! TTGACAATAGCGGACATCAT+TGG + chr8.1:24672395-24672414 MS.gene44418:CDS 40.0%
!! TTGGTTTTTGACCTCAAGGT+TGG - chr8.1:24668601-24668620 None:intergenic 40.0%
AATGGATGCAATCCAACAGG+AGG - chr8.1:24669701-24669720 None:intergenic 45.0%
AGGGCAAAGAAGGGTGAATT+AGG - chr8.1:24670787-24670806 None:intergenic 45.0%
ATGGATGCAATCCAACAGGA+GGG - chr8.1:24669700-24669719 None:intergenic 45.0%
CAGGAGAGCAACAAGAAGAA+CGG + chr8.1:24668661-24668680 MS.gene44418:CDS 45.0%
CCACAAGAAATCTGTACGAG+TGG + chr8.1:24672488-24672507 MS.gene44418:CDS 45.0%
CCACTCGTACAGATTTCTTG+TGG - chr8.1:24672491-24672510 None:intergenic 45.0%
CCATAGTGCAACATTTGCGA+AGG - chr8.1:24671559-24671578 None:intergenic 45.0%
CCTTCGCAAATGTTGCACTA+TGG + chr8.1:24671556-24671575 MS.gene44418:intron 45.0%
CGTAAAGGAGGGAGATAACT+CGG - chr8.1:24672057-24672076 None:intergenic 45.0%
CTAGGGATTAGGGCAAAGAA+GGG - chr8.1:24670796-24670815 None:intergenic 45.0%
CTGCCTTATCTTTCTCACAG+AGG - chr8.1:24669471-24669490 None:intergenic 45.0%
GAACATGAGCATCCAGAAAC+TGG + chr8.1:24670462-24670481 MS.gene44418:CDS 45.0%
GGACGAAATCAAGTTTGGAG+GGG + chr8.1:24672447-24672466 MS.gene44418:CDS 45.0%
GGGCAAAGAAGGGTGAATTA+GGG - chr8.1:24670786-24670805 None:intergenic 45.0%
GTCTGCGGATGTCAATGTTT+CGG + chr8.1:24669336-24669355 MS.gene44418:CDS 45.0%
TATAAGATACCTCGTCTGGG+TGG - chr8.1:24671653-24671672 None:intergenic 45.0%
TATAGCATCACCCAACACTG+AGG - chr8.1:24669215-24669234 None:intergenic 45.0%
TCAGTGATAAGTTGCGACGT+GGG + chr8.1:24670554-24670573 MS.gene44418:intron 45.0%
TGAAGGTTGTACCAACCTTG+AGG + chr8.1:24668587-24668606 MS.gene44418:CDS 45.0%
TGGACCAGTTCATACCTTGA+GGG + chr8.1:24670610-24670629 MS.gene44418:intron 45.0%
TGTTTAGCAGGAGAGGGATA+TGG + chr8.1:24669671-24669690 MS.gene44418:intron 45.0%
TTCAGTGATAAGTTGCGACG+TGG + chr8.1:24670553-24670572 MS.gene44418:intron 45.0%
TTGGACCAGTTCATACCTTG+AGG + chr8.1:24670609-24670628 MS.gene44418:intron 45.0%
! GTGAATGGACTCACACTTGA+AGG + chr8.1:24670195-24670214 MS.gene44418:intron 45.0%
!! GCTAGCCTTAAACGTAAAGG+AGG - chr8.1:24672069-24672088 None:intergenic 45.0%
!! GCTTTGGGTAATGGTAATGG+TGG + chr8.1:24670311-24670330 MS.gene44418:intron 45.0%
!! GGGTAATGGTAATGGTGGTA+AGG + chr8.1:24670316-24670335 MS.gene44418:intron 45.0%
!! TGTACGAGTGGAAGGCATTA+AGG + chr8.1:24672500-24672519 MS.gene44418:CDS 45.0%
!!! AAATTTTAAACTTAATATTT+TGG + chr8.1:24669768-24669787 MS.gene44418:intron 5.0%
!!! TTTAAATATTTCTATAAAAT+AGG + chr8.1:24671195-24671214 MS.gene44418:intron 5.0%
ATAAGTTGCGACGTGGGAAG+AGG + chr8.1:24670560-24670579 MS.gene44418:intron 50.0%
ATATGTGGGGATAAGGAGCG+GGG - chr8.1:24672143-24672162 None:intergenic 50.0%
ATGGACAAGGCCTCAGTGTT+GGG + chr8.1:24669202-24669221 MS.gene44418:CDS 50.0%
CAGTCGCATATGTGGGGATA+AGG - chr8.1:24672150-24672169 None:intergenic 50.0%
CATATGTGGGGATAAGGAGC+GGG - chr8.1:24672144-24672163 None:intergenic 50.0%
GATGGTTATGGCCAAAGCAG+AGG - chr8.1:24669569-24669588 None:intergenic 50.0%
GCATATGTGGGGATAAGGAG+CGG - chr8.1:24672145-24672164 None:intergenic 50.0%
GCTAGGGATTAGGGCAAAGA+AGG - chr8.1:24670797-24670816 None:intergenic 50.0%
GGATATGGATTCCCTCCTGT+TGG + chr8.1:24669686-24669705 MS.gene44418:intron 50.0%
GGCCATAACCATCATTGCTC+AGG + chr8.1:24669576-24669595 MS.gene44418:CDS 50.0%
TAGTCGATCTGGACAGATCC+AGG - chr8.1:24672120-24672139 None:intergenic 50.0%
! ACAGATCCAGGTGCTGCATT+AGG - chr8.1:24672108-24672127 None:intergenic 50.0%
!! CGGGGAAGAATGTGCTGATT+AGG + chr8.1:24669443-24669462 MS.gene44418:CDS 50.0%
!! GAGTGGAAGGCATTAAGGCT+TGG + chr8.1:24672505-24672524 MS.gene44418:CDS 50.0%
CTCGCCCTCAAGGTATGAAC+TGG - chr8.1:24670617-24670636 None:intergenic 55.0%
GATGGACAAGGCCTCAGTGT+TGG + chr8.1:24669201-24669220 MS.gene44418:intron 55.0%
TACGGTCCTAATGCAGCACC+TGG + chr8.1:24672099-24672118 MS.gene44418:CDS 55.0%
AAGGAGCGGGGTAGTCGATC+TGG - chr8.1:24672131-24672150 None:intergenic 60.0%
TGTGCAGTACCACCCAGACG+AGG + chr8.1:24671641-24671660 MS.gene44418:CDS 60.0%
!! CATTGATCGGCTCGCCCTCA+AGG - chr8.1:24670627-24670646 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 24668586 24672579 24668586 ID=MS.gene44418
chr8.1 mRNA 24668586 24672579 24668586 ID=MS.gene44418.t1;Parent=MS.gene44418
chr8.1 exon 24668586 24668804 24668586 ID=MS.gene44418.t1.exon1;Parent=MS.gene44418.t1
chr8.1 CDS 24668586 24668804 24668586 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
chr8.1 exon 24669202 24669597 24669202 ID=MS.gene44418.t1.exon2;Parent=MS.gene44418.t1
chr8.1 CDS 24669202 24669597 24669202 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
chr8.1 exon 24670448 24670507 24670448 ID=MS.gene44418.t1.exon3;Parent=MS.gene44418.t1
chr8.1 CDS 24670448 24670507 24670448 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
chr8.1 exon 24671582 24671662 24671582 ID=MS.gene44418.t1.exon4;Parent=MS.gene44418.t1
chr8.1 CDS 24671582 24671662 24671582 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
chr8.1 exon 24671793 24672227 24671793 ID=MS.gene44418.t1.exon5;Parent=MS.gene44418.t1
chr8.1 CDS 24671793 24672227 24671793 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
chr8.1 exon 24672331 24672579 24672331 ID=MS.gene44418.t1.exon6;Parent=MS.gene44418.t1
chr8.1 CDS 24672331 24672579 24672331 ID=cds.MS.gene44418.t1;Parent=MS.gene44418.t1
Gene Sequence

>MS.gene44418

ATGAAGGTTGTACCAACCTTGAGGTCAAAAACCAAAGTTGTGAACTCAAAGAATGGACCAAGACGTGTAAAGAATCAGGAGAGCAACAAGAAGAACGGTTCATTTTCCAAATCTACAACTCATCACACACCAGATCATATAATTGCTGAGAGAATAAGGAGAGAAAAGATTAGCCAACAGTTCATAGCTCTTTCAGCCCTTATTCCAAACCTCAAAAAGGTTCATTTTTCAATTTATACACATTTGACTCGAACTATATTTTTGTGGGTATGCTAATATGATTCAATTCATATACAATTTGATTACTAAGTTTTTTTTTCTTTAAGATTTAATTTATATTCATTCATAATGTAAAGATTTTTTAGATTGTGCGTCAGTCAATCGGATCAATCATAATCGTTCGATAATTAAAAAAGTTTGACTTTTAATCTTATCATCAGAAACATTTGACTTTATTCTCGTGATTACTTCTAAAATCACGTTGACACACATGATTGATTGTGATTTGGCGTATCAAAATTAAACTACAATCACATTGACACATGTGATTGATTGCGATTTATTGACGGCGTAACAAAATTAAACTATACTAATAATGTACTCCATATGTTTGTAGATGGACAAGGCCTCAGTGTTGGGTGATGCTATAAAGTATGTGAAGGAGCTTAAGGAACAAGTAAAACTGTTGGAGGAGCAAAGCAAAAGCAAAAGTGTAGAACCGGTTACGGTTGTGAAGAAATTGTCTGAACTGTCTGCGGATGTCAATGTTTCGGACACTTCCTCAAATTCATGTAATGGAAACTCTGATGAAACATCAAAAACAAATCTGTCACTACCTGAAGTTAAAGCTAGTTTGTCGGGGAAGAATGTGCTGATTAGGATCCTCTGTGAGAAAGATAAGGCAGTGATGGGGAATGTATATAGAGAAATAGAGAAACTTGGTCTCTTGGTTATCAATGCCAGTTCCTTTTCATTTGGTTCCTCTGCTTTGGCCATAACCATCATTGCTCAGGTACATTTTTAATTAAGGACTATATGGTTATGATAATGATACTACTTTCGATAAATAAAAATGTCATAAAATGTGTTTAGCAGGAGAGGGATATGGATTCCCTCCTGTTGGATTGCATCCATTCATGATGTTTGCGTATTTGTGGTTGTTTGATCAAAATTGAACGATTCAAATTTTAAACTTAATATTTTGGATTTTACGAAAATATTTTTTTACTGTCCAATCTTAATGCAATTTTTATTGATGTGTTGACTAGGAATACAAGTGAGAGATGGAAATCAATTTCTTAGCGGGAGGAACCTTGCATGCTAATTAAAAACAGTTGACCAACATAATGTCTTAAGAGTGTTTTGTTAAAATGTAGTGTCAAATTGACTTGGTTGCTTAAAGTTCAAAGTAATATTACTCGGTGTTTTTAGTTTTTCGTAAGGATCCAATAATGGTATCATGGTCAATAGTTTGATTGAGTCCTTATACCATTTTTTTAACAATGTGTTGTGGTCAATTGGTGTGTCTCGTTGATGTTGTATTAACGGTGTTGCAAATGAATGTGAATGAAAAAGTTTTAGTTTGCAAGGAAATCATATACTTAGATGAGTGAATGGACTCACACTTGAAGGAGAGATTGTTAAGTGTGAGTAGTAATTTCATATTAAAAAATGGATTTAAACATCACAAAAGTTAGAGGACTCATATACGATCGATGTATTAATGCTTTGGGTAATGGTAATGGTGGTAAGGAGAGCAATGTCTCATCATTTGTTGTGTATATTTTTTTATAAGAGAGTAATATCTCATCACTATGGACTCAGAATAATTGACGTAATTGTGTCTTTATGGTGTTTGGCAGATGGAGAATGAATTGAACATGAGCATCCAGAAACTGGCAAAAAAACTGAGAGTTGGACTGGTGCAATTGAATTAGATTGTTGTGTGTGTAAGATGAGATCATGAATTCAGTGATAAGTTGCGACGTGGGAAGAGGAAATCATGTACACGCAGTAAACTTGTTTGGACCAGTTCATACCTTGAGGGCGAGCCGATCAATGCGCATTTATAGCCTCATATTTTATTACTTCCTCTATCTCATAATATGTGTCCTATTTGAGTTTTATATGGTTGTTAAAGAAATGATCAGTGATATCCTTTATTATAAAAGCTAGACTTGTTTGGCTATCATGTTTGCCCTAATTCACCCTTCTTTGCCCTAATCCCTAGCATTTTTCCCTCCTAAACATGTACTAATGACTAATCTAATCTCATCCGTTCAACCTCTATTTTCACACTTGATCACACCCGTTGATTCAAAGTCTACAAATTTTCTCTCATATTACTTAGTTAATGCAATTGAAGACGTATTTAATGCAAATAAAGTCGGTCAACAATCCACTAACTAAATGCTGATCAAAAGGTGTTCTTCGTACTGACATTACAATGTTTCACACATACATTTTTTAAATAAAGTCGAAATTATATGTCATTAACTATAATTAAATTGCTATAACTAATTGCTTTAAATAAAATGATATGCCACTGTCCAAAAAGTATTTCGACTTCTCTCAAAATAAAAAAAGTATTTCGACTAAATAGGAATTACTTTAAATATTTCTATAAAATAGGCTGGTAATTAATTGCTTTTAATTTTCGCAATGAATGGGAAATACTACCAATAGAAGCACAACGGTCATTCACATTAATTAACATTCTATAAAATGTTGTCTTCTCCTTATAACTTTGGTGCATATTTTCGAATCAAAGCCTTAAAAAAAATATGATCCTGTAATTATATGATGTTAGACCTTACGACAGCAAAATATATCATGGATTTCGCTCTATACTCATGCTCATGTATATTTTGTTTAATAGAAACTTTATGTTAATATCATCTGATTACTCTTATTTTTTGATGAGTGAAATATACCGTAGCTATGGAACAGCAATAATATATTAACATTGTTCCTTCGCAAATGTTGCACTATGGGAGTATCACGTTGAAGATCCTGCATTTATGCAACTGTTGAAGCTAAAGTGGCGCACATATCATGTGCAGTACCACCCAGACGAGGTATCTTATACAAAAAATCCTGGAACTCAATCATACTGTTAGAAAGTAAAATAGAAGAAAGAAAATATTTTGCTATTTAGTTGCTATAAAGAGCATAATTGATTTATTTTGATACATGTTTCCAATACAGACACATTTGATATTGGGTACAACTCTCAACAAAGAGATAGACAATGAGTTGAATAGGTATGTCACTTTAATCGATCCAGGACTCAATCAATTTGAGGTTGTCGTGGATAAATATGATGGAAAGCTGTATTTCTCTCATGGAATAGAAGAACTGAACAAGGCCTTTAAATTAGAGCATGGTGGCTGGATATCTCTGATCTATGCTTTCCCTCAACTTTTTGTGATTCGGGTTCACGATCGCCATAACTATGAAATCAAGTATCCGAGTTATCTCCCTCCTTTACGTTTAAGGCTAGCACAATTAATTTACGGTCCTAATGCAGCACCTGGATCTGTCCAGATCGACTACCCCGCTCCTTATCCCCACATATGCGACTGTTTTAAATTTAGTGTCACCAAATGGTTAACACTTGCAGATGTAACATCTGATCAACTGGTATACAATCTTTATAGATTTAGTCACATATTTCAGTTTGTTATCTGTCAATGTCGATTTATTTATAAGATCTCATATGTGTCTGTTTTCTTATTTTACATAGACTTTCTCCTATGCTAAAGTATTTGATAGCATTGTTGACAAGAATTGCAAAGAATTGGCAATTATTGACAATAGCGGACATCATTGGTTGTTCAATGTGGTCATTCTTTTTGACAAGGACGAAATCAAGTTTGGAGGGGATTGGAAAATGTTTTGCACCACAAGAAATCTGTACGAGTGGAAGGCATTAAGGCTTGGAGCAAAAACTTTGGCCCCCAATGCATTCCTATTTTGCACATTTGATTTTTAG
Protein sequence

>MS.gene44418.t1

MKVVPTLRSKTKVVNSKNGPRRVKNQESNKKNGSFSKSTTHHTPDHIIAERIRREKISQQFIALSALIPNLKKMDKASVLGDAIKYVKELKEQVKLLEEQSKSKSVEPVTVVKKLSELSADVNVSDTSSNSCNGNSDETSKTNLSLPEVKASLSGKNVLIRILCEKDKAVMGNVYREIEKLGLLVINASSFSFGSSALAITIIAQMENELNMSIQKLAKKLRVGLYHVEDPAFMQLLKLKWRTYHVQYHPDETHLILGTTLNKEIDNELNRYVTLIDPGLNQFEVVVDKYDGKLYFSHGIEELNKAFKLEHGGWISLIYAFPQLFVIRVHDRHNYEIKYPSYLPPLRLRLAQLIYGPNAAPGSVQIDYPAPYPHICDCFKFSVTKWLTLADVTSDQLTFSYAKVFDSIVDKNCKELAIIDNSGHHWLFNVVILFDKDEIKFGGDWKMFCTTRNLYEWKALRLGAKTLAPNAFLFCTFDF