AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44420.t1 MTR_4g097940 83.438 320 45 3 1 319 16 328 0.0 537
MS.gene44420.t1 MTR_4g097920 81.875 320 50 3 1 319 1 313 0.0 531
MS.gene44420.t1 MTR_4g097920 81.875 320 50 3 1 319 16 328 0.0 530
MS.gene44420.t1 MTR_5g014640 44.023 343 129 11 1 315 15 322 1.35e-75 235
MS.gene44420.t1 MTR_4g092700 49.091 220 103 5 105 319 118 333 7.60e-57 187
MS.gene44420.t1 MTR_2g010450 48.990 198 89 4 118 311 156 345 5.21e-52 175
MS.gene44420.t1 MTR_5g014600 45.064 233 102 8 105 315 105 333 1.11e-50 171
MS.gene44420.t1 MTR_5g014520 44.583 240 126 4 77 311 87 324 1.52e-47 163
MS.gene44420.t1 MTR_0011s0210 49.123 171 83 2 144 311 181 350 2.30e-47 163
MS.gene44420.t1 MTR_2g104550 46.354 192 96 3 129 315 121 310 5.55e-47 161
MS.gene44420.t1 MTR_8g009020 44.149 188 92 4 139 315 136 321 7.86e-47 161
MS.gene44420.t1 MTR_0011s0260 49.123 171 83 2 144 311 181 350 3.45e-46 160
MS.gene44420.t1 MTR_6g047570 46.821 173 88 3 142 311 155 326 4.43e-46 159
MS.gene44420.t1 MTR_0250s0040 46.512 172 88 3 143 311 85 255 5.62e-46 157
MS.gene44420.t1 MTR_0246s0020 43.128 211 96 6 110 311 174 369 6.37e-44 154
MS.gene44420.t1 MTR_4g009110 46.237 186 89 5 138 315 166 348 1.70e-42 150
MS.gene44420.t1 MTR_4g067010 44.693 179 94 3 143 319 144 319 7.18e-42 148
MS.gene44420.t1 MTR_5g014560 45.506 178 92 3 145 318 130 306 1.67e-41 146
MS.gene44420.t1 MTR_4g009540 41.935 186 98 3 139 315 145 329 1.79e-41 147
MS.gene44420.t1 MTR_4g066460 43.689 206 109 4 114 315 121 323 7.23e-41 145
MS.gene44420.t1 MTR_0246s0050 41.436 181 101 2 131 311 136 311 7.83e-41 145
MS.gene44420.t1 MTR_4g098035 41.436 181 101 2 131 311 157 332 1.37e-40 145
MS.gene44420.t1 MTR_2g104490 42.553 188 106 2 129 315 120 306 3.59e-40 143
MS.gene44420.t1 MTR_0246s0020 42.857 196 88 6 110 296 174 354 2.67e-38 139
MS.gene44420.t1 MTR_4g097950 47.027 185 83 4 146 318 70 251 2.92e-37 134
MS.gene44420.t1 MTR_2g104590 38.462 182 84 3 139 314 62 221 1.99e-35 128
MS.gene44420.t1 MTR_4g066380 39.423 208 122 3 114 319 119 324 2.48e-35 130
MS.gene44420.t1 MTR_7g080780 43.333 180 93 3 137 315 143 314 4.32e-35 130
MS.gene44420.t1 MTR_7g080780 43.333 180 93 3 137 315 156 327 5.03e-35 130
MS.gene44420.t1 MTR_4g009540 40.719 167 89 3 139 296 145 310 1.10e-34 128
MS.gene44420.t1 MTR_8g009010 38.418 177 74 3 139 314 127 269 1.64e-34 128
MS.gene44420.t1 MTR_2g104490 41.379 174 100 2 129 301 120 292 6.44e-34 126
MS.gene44420.t1 MTR_2g104560 36.612 183 110 3 139 318 47 226 2.15e-33 123
MS.gene44420.t1 MTR_8g069740 40.217 184 91 6 120 295 93 265 2.67e-32 121
MS.gene44420.t1 MTR_2g104650 42.308 182 97 5 139 314 87 266 1.16e-30 117
MS.gene44420.t1 MTR_2g104500 38.202 178 99 3 142 315 100 270 1.29e-28 111
MS.gene44420.t1 MTR_2g104530 35.577 208 114 5 115 315 78 272 8.28e-28 109
MS.gene44420.t1 MTR_6g047550 58.333 72 30 0 142 213 169 240 2.84e-21 91.7
MS.gene44420.t1 MTR_4g009510 33.333 168 102 5 149 315 45 203 5.64e-21 89.4
MS.gene44420.t1 MTR_7g451650 36.879 141 81 3 171 311 59 191 9.10e-21 88.6
MS.gene44420.t1 MTR_5g014550 36.154 130 73 3 176 299 54 179 6.46e-19 83.6
MS.gene44420.t1 MTR_4g009420 36.170 141 74 3 171 311 67 191 1.25e-18 82.8
MS.gene44420.t1 MTR_7g451650 36.923 130 74 3 171 300 59 180 3.76e-18 81.3
MS.gene44420.t1 MTR_7g050350 35.036 137 81 3 175 311 1 129 6.60e-18 79.3
MS.gene44420.t1 MTR_7g451650 35.036 137 81 3 175 311 1 129 6.60e-18 79.3
MS.gene44420.t1 MTR_4g009420 36.154 130 67 3 171 300 67 180 4.13e-16 75.9
MS.gene44420.t1 MTR_8g024790 28.713 202 122 7 127 313 270 464 1.45e-15 77.4
MS.gene44420.t1 MTR_8g027495 34.821 112 68 1 142 253 454 560 1.43e-14 74.7
MS.gene44420.t1 MTR_8g067280 31.928 166 95 5 129 280 448 609 1.78e-14 74.3
MS.gene44420.t1 MTR_7g096350 48.750 80 36 2 129 206 341 417 2.33e-14 73.9
MS.gene44420.t1 MTR_8g024770 31.159 138 78 4 127 251 258 391 6.03e-14 72.4
MS.gene44420.t1 MTR_1g072320 57.407 54 23 0 146 199 470 523 7.09e-14 72.8
MS.gene44420.t1 MTR_8g064700 25.581 258 147 6 44 293 178 398 1.45e-13 71.2
MS.gene44420.t1 MTR_7g117670 53.571 56 26 0 146 201 402 457 5.28e-13 69.7
MS.gene44420.t1 MTR_5g030430 31.214 173 87 7 129 280 479 640 8.48e-13 69.3
MS.gene44420.t1 MTR_7g083900 27.222 180 115 3 147 311 287 465 3.36e-12 67.4
MS.gene44420.t1 MTR_7g083900 29.560 159 96 3 147 290 287 444 8.19e-12 66.2
MS.gene44420.t1 MTR_8g098275 40.299 67 40 0 143 209 305 371 1.09e-11 65.9
MS.gene44420.t1 MTR_8g098275 40.299 67 40 0 143 209 398 464 1.12e-11 65.9
MS.gene44420.t1 MTR_8g098275 40.299 67 40 0 143 209 241 307 1.15e-11 65.5
MS.gene44420.t1 MTR_3g498695 29.936 157 96 4 147 290 299 454 1.20e-11 65.5
MS.gene44420.t1 MTR_3g498695 29.936 157 96 4 147 290 299 454 1.25e-11 65.5
MS.gene44420.t1 MTR_4g057270 28.082 146 99 3 148 288 151 295 4.58e-11 63.2
MS.gene44420.t1 MTR_3g030700 28.365 208 118 7 115 303 165 360 6.23e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44420.t1 AT2G22760 50.000 180 82 5 139 311 112 290 3.98e-52 174
MS.gene44420.t1 AT4G37850 43.426 251 114 7 88 319 87 328 1.11e-51 173
MS.gene44420.t1 AT4G37850 43.426 251 114 7 88 319 87 328 1.11e-51 173
MS.gene44420.t1 AT2G22770 47.964 221 93 6 116 315 99 318 5.49e-49 166
MS.gene44420.t1 AT2G22750 43.825 251 108 9 89 318 66 304 2.01e-47 162
MS.gene44420.t1 AT2G22750 43.825 251 108 9 89 318 94 332 2.57e-47 162
MS.gene44420.t1 AT2G22750 43.825 251 108 9 89 318 90 328 2.70e-47 162
MS.gene44420.t1 AT2G22760 50.000 164 75 4 139 296 112 274 8.51e-47 159
MS.gene44420.t1 AT2G22750 43.825 251 107 10 89 318 66 303 5.71e-46 158
MS.gene44420.t1 AT2G22750 43.825 251 107 10 89 318 94 331 6.31e-46 159
MS.gene44420.t1 AT2G46510 50.000 82 38 1 120 201 372 450 5.44e-16 79.0
MS.gene44420.t1 AT1G32640 35.849 159 88 6 129 280 438 589 4.26e-14 73.2
MS.gene44420.t1 AT1G01260 35.135 111 53 2 143 253 430 521 6.19e-14 72.8
MS.gene44420.t1 AT1G01260 35.135 111 53 2 143 253 430 521 6.19e-14 72.8
MS.gene44420.t1 AT1G01260 35.135 111 53 2 143 253 430 521 6.19e-14 72.8
MS.gene44420.t1 AT5G46760 28.061 196 113 5 129 300 401 592 1.33e-13 71.6
MS.gene44420.t1 AT4G17880 32.075 159 93 5 129 280 402 552 2.56e-13 70.9
MS.gene44420.t1 AT1G63650 38.202 89 54 1 141 228 400 488 3.06e-13 70.9
MS.gene44420.t1 AT1G63650 38.202 89 54 1 141 228 400 488 3.06e-13 70.9
MS.gene44420.t1 AT1G63650 38.202 89 54 1 141 228 400 488 3.06e-13 70.9
MS.gene44420.t1 AT1G12860 29.448 163 105 4 147 300 268 429 1.10e-12 68.9
MS.gene44420.t1 AT1G12860 30.719 153 96 4 147 290 268 419 1.22e-12 68.6
MS.gene44420.t1 AT4G16430 44.156 77 39 1 127 199 297 373 1.91e-12 68.2
MS.gene44420.t1 AT4G29930 31.410 156 94 6 147 299 55 200 2.06e-12 66.2
MS.gene44420.t1 AT4G29930 31.013 158 95 6 147 299 55 203 3.31e-12 65.5
MS.gene44420.t1 AT4G29930 32.143 140 83 5 147 283 55 185 5.97e-12 65.1
MS.gene44420.t1 AT4G29930 49.091 55 28 0 148 202 56 110 6.01e-12 63.9
MS.gene44420.t1 AT4G09820 49.180 61 31 0 139 199 356 416 6.44e-12 66.6
MS.gene44420.t1 AT4G29930 48.214 56 29 0 147 202 55 110 1.02e-11 64.7
MS.gene44420.t1 AT5G41315 35.294 85 53 1 146 228 449 533 1.03e-11 66.2
MS.gene44420.t1 AT4G29930 48.214 56 29 0 147 202 55 110 1.05e-11 64.7
MS.gene44420.t1 AT4G29930 48.214 56 29 0 147 202 55 110 1.05e-11 64.7
MS.gene44420.t1 AT5G41315 35.294 85 53 1 146 228 441 525 1.06e-11 66.2
MS.gene44420.t1 AT5G41315 35.294 85 53 1 146 228 446 530 1.08e-11 65.9
MS.gene44420.t1 AT5G57150 45.763 59 32 0 144 202 53 111 1.53e-11 63.5
MS.gene44420.t1 AT5G57150 45.763 59 32 0 144 202 53 111 1.65e-11 63.5
MS.gene44420.t1 AT5G57150 45.763 59 32 0 144 202 52 110 1.88e-11 63.2
MS.gene44420.t1 AT5G67110 37.374 99 55 2 106 198 52 149 2.00e-11 63.2
MS.gene44420.t1 AT5G57150 45.763 59 32 0 144 202 52 110 2.37e-11 63.5
MS.gene44420.t1 AT5G57150 45.763 59 32 0 144 202 53 111 2.65e-11 63.5
MS.gene44420.t1 AT3G26744 31.210 157 94 5 147 290 308 463 3.56e-11 64.3
MS.gene44420.t1 AT3G26744 31.210 157 94 5 147 290 308 463 4.42e-11 63.9
MS.gene44420.t1 AT3G26744 31.210 157 94 5 147 290 308 463 4.42e-11 63.9
MS.gene44420.t1 AT3G26744 31.210 157 94 5 147 290 308 463 4.42e-11 63.9
MS.gene44420.t1 AT5G46830 26.087 253 136 8 60 280 244 477 4.72e-11 63.9

Find 48 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTGAAGCAAGGGTTTC+TGG 0.125232 8.1:+24694948 MS.gene44420:CDS
TATGGAGAAAACTTGAATTT+TGG 0.186505 8.1:+24694267 MS.gene44420:CDS
TGCATTCGAGTTTGATGATA+TGG 0.245986 8.1:+24693984 MS.gene44420:CDS
GCTCTTTCATCTCTTCTTCC+TGG 0.251328 8.1:+24694423 MS.gene44420:CDS
GATGGACAAGGCAACTGTTT+TGG 0.280749 8.1:+24694761 MS.gene44420:intron
TCTTCTTCCTGGACTCAAAA+AGG 0.317343 8.1:+24694434 MS.gene44420:CDS
AGAATTGTTCTGTGTGCTTT+CGG 0.323320 8.1:-24694088 None:intergenic
AGAATATTGCTTGACAATAA+AGG 0.327738 8.1:+24695507 MS.gene44420:CDS
TTCACCCTTGTATCCATGTT+GGG 0.338276 8.1:-24694222 None:intergenic
ACATGATCTTGAGCCTGAAT+AGG 0.366395 8.1:-24694354 None:intergenic
CATGATCTTGAGCCTGAATA+GGG 0.371001 8.1:-24694353 None:intergenic
CTTCACCCTTGTATCCATGT+TGG 0.403393 8.1:-24694223 None:intergenic
AAGAAGCCTAAAACCGAGAT+TGG 0.405458 8.1:+24694243 MS.gene44420:CDS
AATAAATAGAAACCCTATTC+AGG 0.429952 8.1:+24694341 MS.gene44420:CDS
TTGATCAGGACTACTTTCAA+TGG 0.442294 8.1:-24694115 None:intergenic
TGATCAGGACTACTTTCAAT+GGG 0.443342 8.1:-24694114 None:intergenic
ATGAAAGAGCAATGAATCTC+TGG 0.459868 8.1:-24694410 None:intergenic
AAGAATAAGTACTCTCTTGT+GGG 0.460152 8.1:-24694032 None:intergenic
TCAAGATAGTTATTTCCAAA+TGG 0.471769 8.1:-24695083 None:intergenic
TTCTAATTCGTTGAGTATTG+CGG 0.481274 8.1:-24695024 None:intergenic
CCTAAAACCGAGATTGGGTA+TGG 0.484824 8.1:+24694249 MS.gene44420:CDS
CGAATGCATATGAGTCCATT+GGG 0.496199 8.1:-24693969 None:intergenic
TCGAATGCATATGAGTCCAT+TGG 0.498731 8.1:-24693970 None:intergenic
GGGTTCAAAAGAAATGATCT+TGG 0.501611 8.1:-24694202 None:intergenic
TGATATAACCATTGTTACTC+AGG 0.506395 8.1:+24695103 MS.gene44420:CDS
GGTGAAGCAACAGATTGATC+AGG 0.522332 8.1:-24694129 None:intergenic
TCTCGTAAGGAGCATAAGTC+AGG 0.525482 8.1:+24695531 MS.gene44420:CDS
GAAGAATAAGTACTCTCTTG+TGG 0.530988 8.1:-24694033 None:intergenic
TGTTCTGTGTGCTTTCGGAA+TGG 0.533620 8.1:-24694083 None:intergenic
TTGAACCCAACATGGATACA+AGG 0.536627 8.1:+24694217 MS.gene44420:CDS
TGAGTATTGCGGTCGCAGAT+CGG 0.551040 8.1:-24695013 None:intergenic
TTTGGAAGTGTGATATCTCA+AGG 0.551675 8.1:+24694285 MS.gene44420:CDS
AGAAGCCTAAAACCGAGATT+GGG 0.552154 8.1:+24694244 MS.gene44420:CDS
ATCCATTGCGACAGATATTG+TGG 0.561083 8.1:+24694990 MS.gene44420:CDS
AAAACATATGAAGCAACTAC+AGG 0.565504 8.1:+24694794 MS.gene44420:CDS
GGCCACAATATCTGTCGCAA+TGG 0.577566 8.1:-24694992 None:intergenic
TGACAATAAAGGATCTCGTA+AGG 0.599743 8.1:+24695518 MS.gene44420:CDS
TTATTATAAACGAGAGAACA+AGG 0.617033 8.1:+24694311 MS.gene44420:CDS
GGAAAGAGTGAAGACATTGG+AGG 0.623302 8.1:+24694815 MS.gene44420:CDS
ACTTTGCCTGCAGATGGACA+AGG 0.625234 8.1:+24694749 MS.gene44420:intron
TATTGCCGAAAGAAAACGAA+GGG 0.632199 8.1:+24694377 MS.gene44420:CDS
AGAATAAGTACTCTCTTGTG+GGG 0.633544 8.1:-24694031 None:intergenic
TAATGATCATGAATACCCAA+TGG 0.643981 8.1:+24693954 MS.gene44420:CDS
TGAACCCAACATGGATACAA+GGG 0.649172 8.1:+24694218 MS.gene44420:CDS
ACAGGAAAGAGTGAAGACAT+TGG 0.652927 8.1:+24694812 MS.gene44420:CDS
GATCAGGACTACTTTCAATG+GGG 0.707589 8.1:-24694113 None:intergenic
TAGAAGTGCTCTGAACAGTG+AGG 0.733048 8.1:-24695055 None:intergenic
TTATTGCCGAAAGAAAACGA+AGG 0.770641 8.1:+24694376 MS.gene44420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTAAAAATTTTAAGT+TGG - chr8.1:24695250-24695269 None:intergenic 10.0%
!!! ATTTTAATTAGGTTTTAAGT+TGG - chr8.1:24695278-24695297 None:intergenic 15.0%
!!! CAGATTTTATTTAATTTTCA+TGG + chr8.1:24695454-24695473 MS.gene44420:intron 15.0%
!! TCATGTTGATATATTCTATT+TGG + chr8.1:24695360-24695379 MS.gene44420:intron 20.0%
!!! CTCTTATGATCATTTTAATT+AGG - chr8.1:24695289-24695308 None:intergenic 20.0%
!!! GTTTATTTTAAGTTTCTAGT+TGG + chr8.1:24695191-24695210 MS.gene44420:intron 20.0%
! AATAAATAGAAACCCTATTC+AGG + chr8.1:24694341-24694360 MS.gene44420:CDS 25.0%
! AGAATATTGCTTGACAATAA+AGG + chr8.1:24695507-24695526 MS.gene44420:CDS 25.0%
! TTATTATAAACGAGAGAACA+AGG + chr8.1:24694311-24694330 MS.gene44420:CDS 25.0%
!! AAATTTTACCTGAGTAACAA+TGG - chr8.1:24695114-24695133 None:intergenic 25.0%
!! TAAGCCTCTATATTTTATCT+CGG + chr8.1:24695419-24695438 MS.gene44420:intron 25.0%
!! TATGGAGAAAACTTGAATTT+TGG + chr8.1:24694267-24694286 MS.gene44420:CDS 25.0%
!! TCAAGATAGTTATTTCCAAA+TGG - chr8.1:24695086-24695105 None:intergenic 25.0%
!! TTTCAAAGTGAGTAATGTTT+TGG + chr8.1:24695223-24695242 MS.gene44420:intron 25.0%
!! TTTTCCGAGATAAAATATAG+AGG - chr8.1:24695426-24695445 None:intergenic 25.0%
AAAACATATGAAGCAACTAC+AGG + chr8.1:24694794-24694813 MS.gene44420:CDS 30.0%
AACTAGGCTAGAACATAAAA+TGG - chr8.1:24695313-24695332 None:intergenic 30.0%
AAGAATAAGTACTCTCTTGT+GGG - chr8.1:24694035-24694054 None:intergenic 30.0%
AATCTCACACAATTTGATTG+TGG + chr8.1:24694533-24694552 MS.gene44420:intron 30.0%
ACTTGTCTTAATTGCATGTT+TGG + chr8.1:24695156-24695175 MS.gene44420:intron 30.0%
AGTTAGTATAGTTGCAATTG+AGG + chr8.1:24694684-24694703 MS.gene44420:intron 30.0%
ATATTGCATGTTATATGCAG+GGG - chr8.1:24694589-24694608 None:intergenic 30.0%
CATATTGCATGTTATATGCA+GGG - chr8.1:24694590-24694609 None:intergenic 30.0%
CATGAACAAAGAATTCTACT+CGG - chr8.1:24695345-24695364 None:intergenic 30.0%
TAATGATCATGAATACCCAA+TGG + chr8.1:24693954-24693973 MS.gene44420:CDS 30.0%
TGATATAACCATTGTTACTC+AGG + chr8.1:24695103-24695122 MS.gene44420:CDS 30.0%
! AAACCCTTGCTTCAATTTTT+GGG - chr8.1:24694947-24694966 None:intergenic 30.0%
! TTCTAATTCGTTGAGTATTG+CGG - chr8.1:24695027-24695046 None:intergenic 30.0%
!! TTACTCACTTTGAAACAAGT+TGG - chr8.1:24695218-24695237 None:intergenic 30.0%
!!! TGCTGATTCGACTTTTTTAT+CGG - chr8.1:24694850-24694869 None:intergenic 30.0%
AAAATTGAAGCAAGGGTTTC+TGG + chr8.1:24694948-24694967 MS.gene44420:CDS 35.0%
ACAATTTGATTGTGGAGAAG+TGG + chr8.1:24694541-24694560 MS.gene44420:intron 35.0%
ACTCCCAAAAATTGAAGCAA+GGG + chr8.1:24694941-24694960 MS.gene44420:CDS 35.0%
AGAATAAGTACTCTCTTGTG+GGG - chr8.1:24694034-24694053 None:intergenic 35.0%
AGAATTGTTCTGTGTGCTTT+CGG - chr8.1:24694091-24694110 None:intergenic 35.0%
ATGAAAGAGCAATGAATCTC+TGG - chr8.1:24694413-24694432 None:intergenic 35.0%
GAAGAATAAGTACTCTCTTG+TGG - chr8.1:24694036-24694055 None:intergenic 35.0%
GCATATTGCATGTTATATGC+AGG - chr8.1:24694591-24694610 None:intergenic 35.0%
GGGTTCAAAAGAAATGATCT+TGG - chr8.1:24694205-24694224 None:intergenic 35.0%
TATTGCCGAAAGAAAACGAA+GGG + chr8.1:24694377-24694396 MS.gene44420:CDS 35.0%
TGACAATAAAGGATCTCGTA+AGG + chr8.1:24695518-24695537 MS.gene44420:CDS 35.0%
TGATCAGGACTACTTTCAAT+GGG - chr8.1:24694117-24694136 None:intergenic 35.0%
TTATTGCCGAAAGAAAACGA+AGG + chr8.1:24694376-24694395 MS.gene44420:CDS 35.0%
TTGATCAGGACTACTTTCAA+TGG - chr8.1:24694118-24694137 None:intergenic 35.0%
! AGCACTTCTATTTTGCCATT+TGG + chr8.1:24695068-24695087 MS.gene44420:CDS 35.0%
! CATTTCTTTTGAACCCAACA+TGG + chr8.1:24694209-24694228 MS.gene44420:CDS 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG - chr8.1:24694385-24694404 None:intergenic 35.0%
! GAAACCCTTGCTTCAATTTT+TGG - chr8.1:24694948-24694967 None:intergenic 35.0%
! TGCATTCGAGTTTGATGATA+TGG + chr8.1:24693984-24694003 MS.gene44420:CDS 35.0%
! TTTGGAAGTGTGATATCTCA+AGG + chr8.1:24694285-24694304 MS.gene44420:CDS 35.0%
!! CTTCAATTTTTGGGAGTGAT+TGG - chr8.1:24694938-24694957 None:intergenic 35.0%
!!! AGTCTTAGTTTTGTTGGTGT+AGG - chr8.1:24694153-24694172 None:intergenic 35.0%
!!! GACTTGAGTCTTAGTTTTGT+TGG - chr8.1:24694159-24694178 None:intergenic 35.0%
!!! TATAGTACCTTTTTGAGTCC+AGG - chr8.1:24694444-24694463 None:intergenic 35.0%
!!! TTTTTTGTCAAGTAGCCTAG+TGG + chr8.1:24694507-24694526 MS.gene44420:intron 35.0%
AAGAAGCCTAAAACCGAGAT+TGG + chr8.1:24694243-24694262 MS.gene44420:CDS 40.0%
ACAGGAAAGAGTGAAGACAT+TGG + chr8.1:24694812-24694831 MS.gene44420:CDS 40.0%
ACATGATCTTGAGCCTGAAT+AGG - chr8.1:24694357-24694376 None:intergenic 40.0%
AGAAGCCTAAAACCGAGATT+GGG + chr8.1:24694244-24694263 MS.gene44420:CDS 40.0%
ATCCATTGCGACAGATATTG+TGG + chr8.1:24694990-24695009 MS.gene44420:CDS 40.0%
ATGTTTACTTTGCCTGCAGA+TGG + chr8.1:24694743-24694762 MS.gene44420:intron 40.0%
CACTCCCAAAAATTGAAGCA+AGG + chr8.1:24694940-24694959 MS.gene44420:CDS 40.0%
CATGATCTTGAGCCTGAATA+GGG - chr8.1:24694356-24694375 None:intergenic 40.0%
CGAATGCATATGAGTCCATT+GGG - chr8.1:24693972-24693991 None:intergenic 40.0%
GATCAGGACTACTTTCAATG+GGG - chr8.1:24694116-24694135 None:intergenic 40.0%
TACTCGGCACAAGAAAAACT+AGG - chr8.1:24695329-24695348 None:intergenic 40.0%
TCGAATGCATATGAGTCCAT+TGG - chr8.1:24693973-24693992 None:intergenic 40.0%
TCTTCTTCCTGGACTCAAAA+AGG + chr8.1:24694434-24694453 MS.gene44420:CDS 40.0%
TGAACCCAACATGGATACAA+GGG + chr8.1:24694218-24694237 MS.gene44420:CDS 40.0%
TTCACCCTTGTATCCATGTT+GGG - chr8.1:24694225-24694244 None:intergenic 40.0%
TTGAACCCAACATGGATACA+AGG + chr8.1:24694217-24694236 MS.gene44420:CDS 40.0%
TTGTGTGAGATTTAGCCACT+AGG - chr8.1:24694525-24694544 None:intergenic 40.0%
! GTTTTCTCCATACCCAATCT+CGG - chr8.1:24694259-24694278 None:intergenic 40.0%
CCTAAAACCGAGATTGGGTA+TGG + chr8.1:24694249-24694268 MS.gene44420:CDS 45.0%
CTTCACCCTTGTATCCATGT+TGG - chr8.1:24694226-24694245 None:intergenic 45.0%
GCTCTTTCATCTCTTCTTCC+TGG + chr8.1:24694423-24694442 MS.gene44420:CDS 45.0%
GGAAAGAGTGAAGACATTGG+AGG + chr8.1:24694815-24694834 MS.gene44420:CDS 45.0%
GGTGAAGCAACAGATTGATC+AGG - chr8.1:24694132-24694151 None:intergenic 45.0%
GTTTATATCCCCGTGAGCTT+AGG - chr8.1:24694625-24694644 None:intergenic 45.0%
TGTTCTGTGTGCTTTCGGAA+TGG - chr8.1:24694086-24694105 None:intergenic 45.0%
TTGCATGTTATATGCAGGGG+CGG - chr8.1:24694586-24694605 None:intergenic 45.0%
! CCATACCCAATCTCGGTTTT+AGG - chr8.1:24694252-24694271 None:intergenic 45.0%
! GATGGACAAGGCAACTGTTT+TGG + chr8.1:24694761-24694780 MS.gene44420:intron 45.0%
! GGAGCATAAGTCAGGTTTTG+AGG + chr8.1:24695539-24695558 MS.gene44420:CDS 45.0%
! GTCTGAATTTTCGTCGCTAG+AGG - chr8.1:24694916-24694935 None:intergenic 45.0%
! TCTCGTAAGGAGCATAAGTC+AGG + chr8.1:24695531-24695550 MS.gene44420:CDS 45.0%
! TGTGGAGAAGTGGAGTGTTA+GGG + chr8.1:24694551-24694570 MS.gene44420:intron 45.0%
! TTGTGGAGAAGTGGAGTGTT+AGG + chr8.1:24694550-24694569 MS.gene44420:intron 45.0%
!! TAGAAGTGCTCTGAACAGTG+AGG - chr8.1:24695058-24695077 None:intergenic 45.0%
ACTTTGCCTGCAGATGGACA+AGG + chr8.1:24694749-24694768 MS.gene44420:intron 50.0%
GGCCACAATATCTGTCGCAA+TGG - chr8.1:24694995-24695014 None:intergenic 50.0%
TCCCAACTGACCTAAGCTCA+CGG + chr8.1:24694612-24694631 MS.gene44420:intron 50.0%
TGCATGTTATATGCAGGGGC+GGG - chr8.1:24694585-24694604 None:intergenic 50.0%
! GTGGAGAAGTGGAGTGTTAG+GGG + chr8.1:24694552-24694571 MS.gene44420:intron 50.0%
! TGAGTATTGCGGTCGCAGAT+CGG - chr8.1:24695016-24695035 None:intergenic 50.0%
CAGTTGCCTTGTCCATCTGC+AGG - chr8.1:24694758-24694777 None:intergenic 55.0%
CCAACTGACCTAAGCTCACG+GGG + chr8.1:24694614-24694633 MS.gene44420:intron 55.0%
CCCAACTGACCTAAGCTCAC+GGG + chr8.1:24694613-24694632 MS.gene44420:intron 55.0%
CCCGTGAGCTTAGGTCAGTT+GGG - chr8.1:24694616-24694635 None:intergenic 55.0%
GCATGTTATATGCAGGGGCG+GGG - chr8.1:24694584-24694603 None:intergenic 55.0%
GTGAGCTTAGGTCAGTTGGG+AGG - chr8.1:24694613-24694632 None:intergenic 55.0%
CCCCGTGAGCTTAGGTCAGT+TGG - chr8.1:24694617-24694636 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 24693934 24695573 24693934 ID=MS.gene44420
chr8.1 mRNA 24693934 24695573 24693934 ID=MS.gene44420.t1;Parent=MS.gene44420
chr8.1 exon 24693934 24694455 24693934 ID=MS.gene44420.t1.exon1;Parent=MS.gene44420.t1
chr8.1 CDS 24693934 24694455 24693934 ID=cds.MS.gene44420.t1;Parent=MS.gene44420.t1
chr8.1 exon 24694762 24695124 24694762 ID=MS.gene44420.t1.exon2;Parent=MS.gene44420.t1
chr8.1 CDS 24694762 24695124 24694762 ID=cds.MS.gene44420.t1;Parent=MS.gene44420.t1
chr8.1 exon 24695499 24695573 24695499 ID=MS.gene44420.t1.exon3;Parent=MS.gene44420.t1
chr8.1 CDS 24695499 24695573 24695499 ID=cds.MS.gene44420.t1;Parent=MS.gene44420.t1
Gene Sequence

>MS.gene44420

ATGGAGGACACTACTTTGTTTAATGATCATGAATACCCAATGGACTCATATGCATTCGAGTTTGATGATATGGCATACTTCAAGTCTTTCTCAGCATCCCCACAAGAGAGTACTTATTCTTCTCATAACAACAACAGCAGCAAGCGAATCCATTCCGAAAGCACACAGAACAATTCTTTCCCCATTGAAAGTAGTCCTGATCAATCTGTTGCTTCACCTACACCAACAAAACTAAGACTCAAGTCTTCTTCATCTTCATCTTCATCTTCCAAGATCATTTCTTTTGAACCCAACATGGATACAAGGGTGAAGAAGCCTAAAACCGAGATTGGGTATGGAGAAAACTTGAATTTTGGAAGTGTGATATCTCAAGGTGATTATTATAAACGAGAGAACAAGGTTGCTGCAATAAATAGAAACCCTATTCAGGCTCAAGATCATGTTATTGCCGAAAGAAAACGAAGGGAAAAGCTTAACCAGAGATTCATTGCTCTTTCATCTCTTCTTCCTGGACTCAAAAAGGTACTATATATTATAATGAAATCTCATGCACATAAATATGCGATCTTTTTTTTTTTTGTCAAGTAGCCTAGTGGCTAAATCTCACACAATTTGATTGTGGAGAAGTGGAGTGTTAGGGGTTCAATCCCCGCCCCTGCATATAACATGCAATATGCCTCCCAACTGACCTAAGCTCACGGGGATATAAACATGTGTTCTTTAATATTGAGAGAAATTACATGCAAATTAAGTTAGTATAGTTGCAATTGAGGTCTATCAAGTATACAAATATTATTTAATATTAATCAATGTTTACTTTGCCTGCAGATGGACAAGGCAACTGTTTTGGAAGATGCTATAAAACATATGAAGCAACTACAGGAAAGAGTGAAGACATTGGAGGAACAAGTAGCCGATAAAAAAGTCGAATCAGCAGTTTTTGTGAAGAGATCTATTCTCTTCGACGAAGATGAAAGCTCCTCTAGCGACGAAAATTCAGACCAATCACTCCCAAAAATTGAAGCAAGGGTTTCTGGAAAAGACATGCTCATCAGAATCCATTGCGACAGATATTGTGGCCGATCTGCGACCGCAATACTCAACGAATTAGAAAAGCTTCGCCTCACTGTTCAGAGCACTTCTATTTTGCCATTTGGAAATAACTATCTTGATATAACCATTGTTACTCAGGTAAAATTTATTCAAATAGACATGAGTTTATACTTGTCTTAATTGCATGTTTGGTTCGTTCTTGATGTTTATTTTAAGTTTCTAGTTGGTCCAACTTGTTTCAAAGTGAGTAATGTTTTGGACCAACTTAAAATTTTTAAAACTTAAAGACCAACTTAAAACCTAATTAAAATGATCATAAGAGACCATTTTATGTTCTAGCCTAGTTTTTCTTGTGCCGAGTAGAATTCTTTGTTCATGTTGATATATTCTATTTGGCTCGTTAAAAAAAACATAAGAAATCAAACATGCAATTAAGCCTCTATATTTTATCTCGGAAAAATTAGTGACAGATTTTATTTAATTTTCATGGTTAATTTAATTTTTTCTTGCAGATGAATAAAGAATATTGCTTGACAATAAAGGATCTCGTAAGGAGCATAAGTCAGGTTTTGAGGCAGCTCATTTGA
Protein sequence

>MS.gene44420.t1

MEDTTLFNDHEYPMDSYAFEFDDMAYFKSFSASPQESTYSSHNNNSSKRIHSESTQNNSFPIESSPDQSVASPTPTKLRLKSSSSSSSSSKIISFEPNMDTRVKKPKTEIGYGENLNFGSVISQGDYYKRENKVAAINRNPIQAQDHVIAERKRREKLNQRFIALSSLLPGLKKMDKATVLEDAIKHMKQLQERVKTLEEQVADKKVESAVFVKRSILFDEDESSSSDENSDQSLPKIEARVSGKDMLIRIHCDRYCGRSATAILNELEKLRLTVQSTSILPFGNNYLDITIVTQMNKEYCLTIKDLVRSISQVLRQLI