AlfalfaGEDB | MS.gene44442

M. sativa cultivar XinJiangDaYe / MS.gene44442

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44442.t1	XP_003608557.1	94.3	174	10	0	1	174	1	174	1.10E-83	319.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44442.t1	G7JF85	94.3	174	10	0	1	174	1	174	7.9e-84	319.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene44442.t1	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44442.t1	MTR_4g097440	94.253	174	10	0	1	174	1	174	8.64e-121	338
MS.gene44442.t1	MTR_5g015090	56.322	174	59	6	1	174	1	157	3.97e-56	174
MS.gene44442.t1	MTR_3g103360	47.619	84	44	0	71	154	52	135	3.54e-18	76.6
MS.gene44442.t1	MTR_1g017570	65.152	66	23	0	74	139	59	124	2.48e-17	74.7
MS.gene44442.t1	MTR_4g070860	41.935	93	54	0	62	154	17	109	5.31e-17	74.3
MS.gene44442.t1	MTR_1g022495	38.739	111	66	1	44	154	1	109	1.03e-15	70.9
MS.gene44442.t1	MTR_3g117120	41.053	95	49	1	67	154	15	109	3.61e-15	69.3
MS.gene44442.t1	MTR_6g016375	43.182	88	50	0	63	150	78	165	6.64e-15	69.7
MS.gene44442.t1	MTR_7g029400	34.091	132	75	2	16	147	37	156	6.76e-15	69.3
MS.gene44442.t1	MTR_8g015250	44.706	85	47	0	63	147	62	146	1.28e-14	68.6
MS.gene44442.t1	MTR_7g017880	41.176	85	50	0	63	147	75	159	1.52e-13	65.9
MS.gene44442.t1	MTR_8g086020	38.554	83	51	0	72	154	32	114	5.50e-13	63.5
MS.gene44442.t1	MTR_1g087040	33.333	96	61	1	72	164	24	119	9.34e-12	60.1
MS.gene44442.t1	MTR_3g112220	37.079	89	55	1	72	160	1	88	4.31e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44442.t1	AT2G22850	34.579	214	86	5	1	160	1	214	8.49e-30	109
MS.gene44442.t1	AT2G22850	34.579	214	86	5	1	160	1	214	8.49e-30	109
MS.gene44442.t1	AT3G49760	37.736	159	88	4	1	155	1	152	1.90e-28	103
MS.gene44442.t1	AT4G37730	35.681	213	72	6	1	148	58	270	5.87e-26	100
MS.gene44442.t1	AT5G38800	35.152	165	78	4	2	163	18	156	2.91e-17	75.1
MS.gene44442.t1	AT2G18160	45.783	83	45	0	72	154	28	110	5.38e-17	74.7
MS.gene44442.t1	AT1G75390	40.860	93	55	0	62	154	28	120	1.35e-15	70.9
MS.gene44442.t1	AT3G30530	34.000	150	88	2	1	150	18	156	1.41e-15	70.9
MS.gene44442.t1	AT5G15830	43.373	83	47	0	71	153	70	152	5.50e-14	67.0
MS.gene44442.t1	AT4G34590	38.372	86	53	0	69	154	21	106	1.81e-13	65.1
MS.gene44442.t1	AT1G75390	43.590	78	44	0	62	139	28	105	2.42e-13	63.9
MS.gene44442.t1	AT1G13600	44.706	85	47	0	63	147	74	158	4.24e-13	64.7
MS.gene44442.t1	AT2G04038	43.299	97	44	2	64	157	60	148	2.03e-12	62.4
MS.gene44442.t1	AT3G62420	37.895	95	57	2	62	154	10	104	8.96e-12	60.1
MS.gene44442.t1	AT5G49450	34.524	84	55	0	72	155	13	96	2.71e-11	58.9

Find 0 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTTGATATCATCTGAAAAT+AGG	+	32967:6566-6585	None:intergenic	25.0%
!!	CAACAACTTTACTCAAATTT+TGG	-	32967:6914-6933	MS.gene44442:CDS	25.0%
	AAAACCGGGAAATGAAAAAT+CGG	-	32967:6805-6824	MS.gene44442:CDS	30.0%
	AAAGACATGTTGAGAATCTA+AGG	-	32967:6757-6776	MS.gene44442:CDS	30.0%
	AACTTGAAGTGATAGGTTTA+GGG	+	32967:6596-6615	None:intergenic	30.0%
!!	GAGTAAAGTTGTTGATTCTT+TGG	+	32967:6908-6927	None:intergenic	30.0%
!!	TCATTTTGTTTCACTTGAAC+CGG	-	32967:6835-6854	MS.gene44442:CDS	30.0%
	ACAAGTGTAGAATGGAAAAC+CGG	-	32967:6790-6809	MS.gene44442:CDS	35.0%
	ATGGACATGTAACACTACTA+TGG	-	32967:6968-6987	MS.gene44442:CDS	35.0%
	GAACTTGAAGTGATAGGTTT+AGG	+	32967:6597-6616	None:intergenic	35.0%
	TGGAGAAAGGTTGAAAATGT+TGG	+	32967:6944-6963	None:intergenic	35.0%
!	AAACCTATCACTTCAAGTTC+CGG	-	32967:6597-6616	MS.gene44442:CDS	35.0%
!!	ATTCATTTTCGGTTCGTATC+CGG	+	32967:6857-6876	None:intergenic	35.0%
!!	GTAACCGATTTTTCATTTCC+CGG	+	32967:6812-6831	None:intergenic	35.0%
	AATAGGTCGTGAAAATCCCA+CGG	+	32967:6549-6568	None:intergenic	40.0%
	ACCTCATGAGAAACGTAAAC+CGG	-	32967:6638-6657	MS.gene44442:CDS	40.0%
	AGTGTTACATGTCCATGAAG+TGG	+	32967:6964-6983	None:intergenic	40.0%
	CAAGTGTAGAATGGAAAACC+GGG	-	32967:6791-6810	MS.gene44442:CDS	40.0%
	CCAGTTGAACAAGTGTAGAA+TGG	-	32967:6782-6801	MS.gene44442:CDS	40.0%
	CCATTCTACACTTGTTCAAC+TGG	+	32967:6785-6804	None:intergenic	40.0%
	GAACCGAAAATGAATCGCTT+CGG	-	32967:6862-6881	MS.gene44442:CDS	40.0%
	GACATGTAACACTACTATGG+TGG	-	32967:6971-6990	MS.gene44442:CDS	40.0%
	GTTTACGTTTCTCATGAGGT+TGG	+	32967:6638-6657	None:intergenic	40.0%
	TTCAACCTTTCTCCACTTCA+TGG	-	32967:6949-6968	MS.gene44442:CDS	40.0%
!	AGACCGAAGCGATTCATTTT+CGG	+	32967:6868-6887	None:intergenic	40.0%
!	GTTGATTCTTTGGTTCAGCA+TGG	+	32967:6898-6917	None:intergenic	40.0%
!!	TTGGTTTGGTTCATCTGAAC+CGG	+	32967:6619-6638	None:intergenic	40.0%
	CATGTCCATGAAGTGGAGAA+AGG	+	32967:6957-6976	None:intergenic	45.0%
	GAACCGGAACTTGAAGTGAT+AGG	+	32967:6603-6622	None:intergenic	45.0%
	TCCGGTTTACGTTTCTCATG+AGG	+	32967:6642-6661	None:intergenic	45.0%
!	AGTGATAGGTTTAGGGCTTG+AGG	+	32967:6589-6608	None:intergenic	45.0%
!	CGTTTCTCATGAGGTTGGTT+TGG	+	32967:6633-6652	None:intergenic	45.0%
!	GTGATAGGTTTAGGGCTTGA+GGG	+	32967:6588-6607	None:intergenic	45.0%
!!	AGAATGGGTTGTCGAGAAGA+GGG	+	32967:6503-6522	None:intergenic	45.0%
	ACTCAGACGAACCGAACCAA+GGG	-	32967:6661-6680	MS.gene44442:CDS	50.0%
!!	GAGAATGGGTTGTCGAGAAG+AGG	+	32967:6504-6523	None:intergenic	50.0%
	AACCCATTCTCCGCCTTCCA+CGG	-	32967:6513-6532	MS.gene44442:CDS	55.0%
	AGGTCGTGAAAATCCCACGG+CGG	+	32967:6546-6565	None:intergenic	55.0%
	CCGACGCATGATATCGAACC+GGG	-	32967:6704-6723	MS.gene44442:CDS	55.0%
	CTCAGACGAACCGAACCAAG+GGG	-	32967:6662-6681	MS.gene44442:CDS	55.0%
	CTTACGCATCCGTGACCTTC+GGG	+	32967:6736-6755	None:intergenic	55.0%
	GACTCAGACGAACCGAACCA+AGG	-	32967:6660-6679	MS.gene44442:CDS	55.0%
	GCCGACGCATGATATCGAAC+CGG	-	32967:6703-6722	MS.gene44442:CDS	55.0%
	GCTTACGCATCCGTGACCTT+CGG	+	32967:6737-6756	None:intergenic	55.0%
	TGGGTTGTCGAGAAGAGGGT+CGG	+	32967:6499-6518	None:intergenic	55.0%
	TTGTCGAGAAGAGGGTCGGA+GGG	+	32967:6495-6514	None:intergenic	55.0%
!	CCCGGTTCGATATCATGCGT+CGG	+	32967:6707-6726	None:intergenic	55.0%
!!	TGGTTCGGTTCGTCTGAGTC+CGG	+	32967:6660-6679	None:intergenic	55.0%
	AGACGAACCGAACCAAGGGG+CGG	-	32967:6665-6684	MS.gene44442:CDS	60.0%
	CACCGTGGAAGGCGGAGAAT+GGG	+	32967:6518-6537	None:intergenic	60.0%
	GGGAATCAGCCCGAAGGTCA+CGG	-	32967:6724-6743	MS.gene44442:CDS	60.0%
	GGTCGTGAAAATCCCACGGC+GGG	+	32967:6545-6564	None:intergenic	60.0%
	GTTGTCGAGAAGAGGGTCGG+AGG	+	32967:6496-6515	None:intergenic	60.0%
	TCATCGACCGCCCCTTGGTT+CGG	+	32967:6675-6694	None:intergenic	60.0%
!	GTGACCTTCGGGCTGATTCC+CGG	+	32967:6725-6744	None:intergenic	60.0%
	AAGAGGGTCGGAGGGAGGGA+GGG	+	32967:6487-6506	None:intergenic	65.0%
	CCACCGTGGAAGGCGGAGAA+TGG	+	32967:6519-6538	None:intergenic	65.0%
	CCATTCTCCGCCTTCCACGG+TGG	-	32967:6516-6535	MS.gene44442:CDS	65.0%
	CGAACCGGGAATCAGCCCGA+AGG	-	32967:6718-6737	MS.gene44442:CDS	65.0%
	CGAGAAGAGGGTCGGAGGGA+GGG	+	32967:6491-6510	None:intergenic	65.0%
	CGGGAAACCACCGTGGAAGG+CGG	+	32967:6526-6545	None:intergenic	65.0%
	TCGAGAAGAGGGTCGGAGGG+AGG	+	32967:6492-6511	None:intergenic	65.0%
	TTCGCTCATCGACCGCCCCT+TGG	+	32967:6680-6699	None:intergenic	65.0%
	CCACGGTGGTTTCCCGCCGT+GGG	-	32967:6530-6549	MS.gene44442:CDS	70.0%
	CGGCGGGAAACCACCGTGGA+AGG	+	32967:6529-6548	None:intergenic	70.0%
	GAAGAGGGTCGGAGGGAGGG+AGG	+	32967:6488-6507	None:intergenic	70.0%
	TCCACGGTGGTTTCCCGCCG+TGG	-	32967:6529-6548	MS.gene44442:CDS	70.0%
	AGGGTCGGAGGGAGGGAGGG+TGG	+	32967:6484-6503	None:intergenic	75.0%
	CCCACGGCGGGAAACCACCG+TGG	+	32967:6533-6552	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
32967	gene	6474	6998	6474	ID=MS.gene44442
32967	mRNA	6474	6998	6474	ID=MS.gene44442.t1;Parent=MS.gene44442
32967	exon	6474	6998	6474	ID=MS.gene44442.t1.exon1;Parent=MS.gene44442.t1
32967	CDS	6474	6998	6474	ID=cds.MS.gene44442.t1;Parent=MS.gene44442.t1

Gene Sequence

>MS.gene44442

ATGCTCTCCACCCTCCCTCCCTCCGACCCTCTTCTCGACAACCCATTCTCCGCCTTCCACGGTGGTTTCCCGCCGTGGGATTTTCACGACCTATTTTCAGATGATATCAAACCCTCAAGCCCTAAACCTATCACTTCAAGTTCCGGTTCAGATGAACCAAACCAACCTCATGAGAAACGTAAACCGGACTCAGACGAACCGAACCAAGGGGCGGTCGATGAGCGAAAGCGCCGACGCATGATATCGAACCGGGAATCAGCCCGAAGGTCACGGATGCGTAAGCAAAGACATGTTGAGAATCTAAGGAACCAGTTGAACAAGTGTAGAATGGAAAACCGGGAAATGAAAAATCGGTTACAATTCATTTTGTTTCACTTGAACCGGATACGAACCGAAAATGAATCGCTTCGGTCTGAGCGAACCATGCTGAACCAAAGAATCAACAACTTTACTCAAATTTTGGTTTGCCAACATTTTCAACCTTTCTCCACTTCATGGACATGTAACACTACTATGGTGGAATAA

Protein sequence

>MS.gene44442.t1

MLSTLPPSDPLLDNPFSAFHGGFPPWDFHDLFSDDIKPSSPKPITSSSGSDEPNQPHEKRKPDSDEPNQGAVDERKRRRMISNRESARRSRMRKQRHVENLRNQLNKCRMENREMKNRLQFILFHLNRIRTENESLRSERTMLNQRINNFTQILVCQHFQPFSTSWTCNTTMVE