Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44792.t1 | XP_013447731.1 | 94.2 | 137 | 6 | 2 | 1 | 136 | 1 | 136 | 1.00E-53 | 219.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44792.t1 | A0A072TXB5 | 94.2 | 137 | 6 | 2 | 1 | 136 | 1 | 136 | 7.4e-54 | 219.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene44792.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44792.t1 | MTR_7g018500 | 92.199 | 141 | 9 | 2 | 1 | 140 | 1 | 140 | 3.19e-66 | 207 |
| MS.gene44792.t1 | MTR_7g018500 | 85.484 | 62 | 8 | 1 | 137 | 197 | 294 | 355 | 2.44e-16 | 76.3 |
| MS.gene44792.t1 | MTR_1g103380 | 40.517 | 116 | 56 | 5 | 42 | 148 | 118 | 229 | 1.35e-12 | 65.9 |
| MS.gene44792.t1 | MTR_6g017055 | 47.619 | 84 | 24 | 3 | 73 | 140 | 67 | 146 | 2.98e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCAACTTATTGCTCTTT+AGG | 0.129653 | 7.4:-81655987 | MS.gene44792:CDS |
| ATCCTGATACACTAACAAAT+TGG | 0.193845 | 7.4:-81655761 | MS.gene44792:CDS |
| GCCCAATTTGTTAGTGTATC+AGG | 0.208804 | 7.4:+81655759 | None:intergenic |
| CTCCAATGTTATCACCAAAT+TGG | 0.217754 | 7.4:-81655032 | MS.gene44792:CDS |
| GTTAGTGTATCAGGATTAAT+TGG | 0.223236 | 7.4:+81655768 | None:intergenic |
| TGTGAAGAAGAAGATGAATT+AGG | 0.330614 | 7.4:+81655945 | None:intergenic |
| TTATTATTAGCATCCCAATT+TGG | 0.341400 | 7.4:+81655018 | None:intergenic |
| TCATGAGAATCACTATTATT+AGG | 0.353829 | 7.4:+81655057 | None:intergenic |
| TTCTTAGCCGGTTTCTTGAC+AGG | 0.362180 | 7.4:+81655654 | None:intergenic |
| TCAGGGGTAAGAAAAGTAAA+AGG | 0.386359 | 7.4:+81655912 | None:intergenic |
| AAAGTTGTTGATGTTGTTGT+TGG | 0.410045 | 7.4:+81655848 | None:intergenic |
| TCCAATGTTATCACCAAATT+GGG | 0.411724 | 7.4:-81655031 | MS.gene44792:CDS |
| CTATGCCTGTCTTTCTTAGC+CGG | 0.420461 | 7.4:+81655642 | None:intergenic |
| TCTTGATATTGCAGAAAAGT+TGG | 0.424180 | 7.4:+81654969 | None:intergenic |
| TCCTGATACACTAACAAATT+GGG | 0.434497 | 7.4:-81655760 | MS.gene44792:CDS |
| GGTGTTGTTAGTGTTGTTGT+TGG | 0.445348 | 7.4:+81655869 | None:intergenic |
| TGATGAGTTGTTTGTGTGTA+GGG | 0.490224 | 7.4:+81655798 | None:intergenic |
| AAAGAAACCTGTCAAGAAAC+CGG | 0.493437 | 7.4:-81655661 | MS.gene44792:CDS |
| AATAACAATGATGCTGTCAC+TGG | 0.496363 | 7.4:-81654997 | MS.gene44792:CDS |
| CCTAAAGAGCAATAAGTTGA+AGG | 0.500471 | 7.4:+81655987 | None:intergenic |
| AGCAAGATTCACACATCTCA+AGG | 0.503482 | 7.4:-81655621 | MS.gene44792:CDS |
| TCAAGAACGAATCTATCATC+AGG | 0.510472 | 7.4:-81654952 | None:intergenic |
| TAGGGAACAGAAATAGCTGC+TGG | 0.529350 | 7.4:+81655816 | None:intergenic |
| GGGGTAAGAAAAGTAAAAGG+AGG | 0.548490 | 7.4:+81655915 | None:intergenic |
| AGAAACCGGCTAAGAAAGAC+AGG | 0.549112 | 7.4:-81655647 | MS.gene44792:CDS |
| TTAGGATTATAACAAGGGTA+AGG | 0.556043 | 7.4:+81655963 | None:intergenic |
| TCCCAATTTGGTGATAACAT+TGG | 0.563066 | 7.4:+81655030 | None:intergenic |
| ATGAATTAGGATTATAACAA+GGG | 0.570279 | 7.4:+81655958 | None:intergenic |
| GGGTAAGAAAAGTAAAAGGA+GGG | 0.575339 | 7.4:+81655916 | None:intergenic |
| ATGATGAGTTGTTTGTGTGT+AGG | 0.581066 | 7.4:+81655797 | None:intergenic |
| GATGAATTAGGATTATAACA+AGG | 0.592158 | 7.4:+81655957 | None:intergenic |
| CTCAAGGGTTGAGAGACAGA+AGG | 0.613193 | 7.4:-81655605 | MS.gene44792:CDS |
| TCAAGGGTTGAGAGACAGAA+GGG | 0.623957 | 7.4:-81655604 | MS.gene44792:intron |
| GCAAGATTCACACATCTCAA+GGG | 0.635283 | 7.4:-81655620 | MS.gene44792:CDS |
| ATGTTGAAACAAGATCTAAG+TGG | 0.698091 | 7.4:-81655717 | MS.gene44792:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATTAGGATTATAACAA+GGG | + | chr7.4:81654991-81655010 | None:intergenic | 20.0% |
| !! | TAAAGATAGAAAGATGAAAA+GGG | - | chr7.4:81655585-81655604 | MS.gene44792:intron | 20.0% |
| ! | CTAAAGATAGAAAGATGAAA+AGG | - | chr7.4:81655584-81655603 | MS.gene44792:intron | 25.0% |
| ! | GATGAATTAGGATTATAACA+AGG | + | chr7.4:81654992-81655011 | None:intergenic | 25.0% |
| ! | TCATGAGAATCACTATTATT+AGG | + | chr7.4:81655892-81655911 | None:intergenic | 25.0% |
| ! | TTATTATTAGCATCCCAATT+TGG | + | chr7.4:81655931-81655950 | None:intergenic | 25.0% |
| !! | TGATATCTTTAACTTCATTG+AGG | - | chr7.4:81655795-81655814 | MS.gene44792:CDS | 25.0% |
| !!! | TGTTGATTTTGATCAATTTC+AGG | + | chr7.4:81655718-81655737 | None:intergenic | 25.0% |
| AAAGTTGTTGATGTTGTTGT+TGG | + | chr7.4:81655101-81655120 | None:intergenic | 30.0% | |
| ATCCTGATACACTAACAAAT+TGG | - | chr7.4:81655185-81655204 | MS.gene44792:intron | 30.0% | |
| ATGTTGAAACAAGATCTAAG+TGG | - | chr7.4:81655229-81655248 | MS.gene44792:intron | 30.0% | |
| GTTAGTGTATCAGGATTAAT+TGG | + | chr7.4:81655181-81655200 | None:intergenic | 30.0% | |
| TCCAATGTTATCACCAAATT+GGG | - | chr7.4:81655915-81655934 | MS.gene44792:CDS | 30.0% | |
| TCCTGATACACTAACAAATT+GGG | - | chr7.4:81655186-81655205 | MS.gene44792:intron | 30.0% | |
| TCTTGATATTGCAGAAAAGT+TGG | + | chr7.4:81655980-81655999 | None:intergenic | 30.0% | |
| TGTGAAGAAGAAGATGAATT+AGG | + | chr7.4:81655004-81655023 | None:intergenic | 30.0% | |
| TTAGGATTATAACAAGGGTA+AGG | + | chr7.4:81654986-81655005 | None:intergenic | 30.0% | |
| ! | GCATCAAATTAGTGTTTTGT+TGG | + | chr7.4:81655870-81655889 | None:intergenic | 30.0% |
| ! | TTTGAAGTTTCATCCTCATT+AGG | + | chr7.4:81655760-81655779 | None:intergenic | 30.0% |
| !!! | TAGAAGAAGCATTTTGATCA+GGG | + | chr7.4:81655054-81655073 | None:intergenic | 30.0% |
| !!! | TTAGAAGAAGCATTTTGATC+AGG | + | chr7.4:81655055-81655074 | None:intergenic | 30.0% |
| AAAGAAACCTGTCAAGAAAC+CGG | - | chr7.4:81655285-81655304 | MS.gene44792:intron | 35.0% | |
| AATAACAATGATGCTGTCAC+TGG | - | chr7.4:81655949-81655968 | MS.gene44792:CDS | 35.0% | |
| ATCTTCTTCATCTCCTTCAT+CGG | + | chr7.4:81655560-81655579 | None:intergenic | 35.0% | |
| ATGATGAGTTGTTTGTGTGT+AGG | + | chr7.4:81655152-81655171 | None:intergenic | 35.0% | |
| CATTAGGCATCAATTGATGA+AGG | + | chr7.4:81655744-81655763 | None:intergenic | 35.0% | |
| CCTTCAACTTATTGCTCTTT+AGG | - | chr7.4:81654959-81654978 | MS.gene44792:CDS | 35.0% | |
| CTCCAATGTTATCACCAAAT+TGG | - | chr7.4:81655914-81655933 | MS.gene44792:CDS | 35.0% | |
| GGGTAAGAAAAGTAAAAGGA+GGG | + | chr7.4:81655033-81655052 | None:intergenic | 35.0% | |
| TCAGGGGTAAGAAAAGTAAA+AGG | + | chr7.4:81655037-81655056 | None:intergenic | 35.0% | |
| TCATCAATTGATGCCTAATG+AGG | - | chr7.4:81655744-81655763 | MS.gene44792:CDS | 35.0% | |
| TCGATCTTCAAGACATGTTA+GGG | - | chr7.4:81655398-81655417 | MS.gene44792:intron | 35.0% | |
| TGATGAGTTGTTTGTGTGTA+GGG | + | chr7.4:81655151-81655170 | None:intergenic | 35.0% | |
| TTCGATCTTCAAGACATGTT+AGG | - | chr7.4:81655397-81655416 | MS.gene44792:intron | 35.0% | |
| ! | CCTAAAGAGCAATAAGTTGA+AGG | + | chr7.4:81654962-81654981 | None:intergenic | 35.0% |
| ! | TCCCAATTTGGTGATAACAT+TGG | + | chr7.4:81655919-81655938 | None:intergenic | 35.0% |
| !!! | AGAAGAAGCATTTTGATCAG+GGG | + | chr7.4:81655053-81655072 | None:intergenic | 35.0% |
| AGAAAACAAGAGCAAGAGCA+AGG | - | chr7.4:81655644-81655663 | MS.gene44792:CDS | 40.0% | |
| AGCAAGAACAACATAGCTTC+TGG | - | chr7.4:81655493-81655512 | MS.gene44792:intron | 40.0% | |
| AGCAAGATTCACACATCTCA+AGG | - | chr7.4:81655325-81655344 | MS.gene44792:intron | 40.0% | |
| GAAAACAAGAGCAAGAGCAA+GGG | - | chr7.4:81655645-81655664 | MS.gene44792:CDS | 40.0% | |
| GCAAGATTCACACATCTCAA+GGG | - | chr7.4:81655326-81655345 | MS.gene44792:intron | 40.0% | |
| GGGGTAAGAAAAGTAAAAGG+AGG | + | chr7.4:81655034-81655053 | None:intergenic | 40.0% | |
| TTCGAAGACGAAGGATTCTA+GGG | - | chr7.4:81655621-81655640 | MS.gene44792:CDS | 40.0% | |
| ! | AAAGATGAAAAGGGCACAGA+AGG | - | chr7.4:81655594-81655613 | MS.gene44792:intron | 40.0% |
| !! | GCCCAATTTGTTAGTGTATC+AGG | + | chr7.4:81655190-81655209 | None:intergenic | 40.0% |
| !! | GGTGTTGTTAGTGTTGTTGT+TGG | + | chr7.4:81655080-81655099 | None:intergenic | 40.0% |
| AAGCCAGCAACACACTTGAA+TGG | - | chr7.4:81655428-81655447 | MS.gene44792:intron | 45.0% | |
| AGAAACCGGCTAAGAAAGAC+AGG | - | chr7.4:81655299-81655318 | MS.gene44792:intron | 45.0% | |
| CTATGCCTGTCTTTCTTAGC+CGG | + | chr7.4:81655307-81655326 | None:intergenic | 45.0% | |
| CTTCGAAGACGAAGGATTCT+AGG | - | chr7.4:81655620-81655639 | MS.gene44792:CDS | 45.0% | |
| GAAGGAATCTTCGAAGACGA+AGG | - | chr7.4:81655612-81655631 | MS.gene44792:CDS | 45.0% | |
| TAGGGAACAGAAATAGCTGC+TGG | + | chr7.4:81655133-81655152 | None:intergenic | 45.0% | |
| TCAAGGGTTGAGAGACAGAA+GGG | - | chr7.4:81655342-81655361 | MS.gene44792:intron | 45.0% | |
| TCTTCTTCGTCTTCCGATGA+AGG | - | chr7.4:81655544-81655563 | MS.gene44792:intron | 45.0% | |
| ! | TTCTTAGCCGGTTTCTTGAC+AGG | + | chr7.4:81655295-81655314 | None:intergenic | 45.0% |
| CTCAAGGGTTGAGAGACAGA+AGG | - | chr7.4:81655341-81655360 | MS.gene44792:intron | 50.0% | |
| GAACTTCCTCGCGATCTCAA+TGG | + | chr7.4:81655377-81655396 | None:intergenic | 50.0% | |
| GACTTTCCATTGAGATCGCG+AGG | - | chr7.4:81655368-81655387 | MS.gene44792:intron | 50.0% | |
| GAGCCATTCAAGTGTGTTGC+TGG | + | chr7.4:81655434-81655453 | None:intergenic | 50.0% | |
| GCTTCTGGCGATGACGACGA+TGG | - | chr7.4:81655508-81655527 | MS.gene44792:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 81654953 | 81656015 | 81654953 | ID=MS.gene44792 |
| chr7.4 | mRNA | 81654953 | 81656015 | 81654953 | ID=MS.gene44792.t1;Parent=MS.gene44792 |
| chr7.4 | exon | 81655605 | 81656015 | 81655605 | ID=MS.gene44792.t1.exon1;Parent=MS.gene44792.t1 |
| chr7.4 | CDS | 81655605 | 81656015 | 81655605 | ID=cds.MS.gene44792.t1;Parent=MS.gene44792.t1 |
| chr7.4 | exon | 81654953 | 81655133 | 81654953 | ID=MS.gene44792.t1.exon2;Parent=MS.gene44792.t1 |
| chr7.4 | CDS | 81654953 | 81655133 | 81654953 | ID=cds.MS.gene44792.t1;Parent=MS.gene44792.t1 |
| Gene Sequence |
| Protein sequence |
