Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44879.t1 | AES98565.1 | 91.8 | 355 | 27 | 2 | 1 | 353 | 1 | 355 | 1.80E-181 | 645.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44879.t1 | Q9FIM4 | 45.3 | 274 | 115 | 6 | 111 | 352 | 95 | 365 | 6.8e-49 | 196.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44879.t1 | G7K6C7 | 91.8 | 355 | 27 | 2 | 1 | 353 | 1 | 355 | 1.3e-181 | 645.2 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44879.t1 | MTR_5g070020 | 94.366 | 355 | 18 | 2 | 1 | 353 | 1 | 355 | 0.0 | 686 |
| MS.gene44879.t1 | MTR_3g463400 | 47.619 | 420 | 133 | 16 | 11 | 352 | 5 | 415 | 1.74e-107 | 322 |
| MS.gene44879.t1 | MTR_4g105660 | 60.563 | 142 | 49 | 1 | 109 | 250 | 168 | 302 | 2.21e-57 | 192 |
| MS.gene44879.t1 | MTR_3g077650 | 64.394 | 132 | 40 | 1 | 119 | 250 | 179 | 303 | 7.38e-56 | 189 |
| MS.gene44879.t1 | MTR_4g088015 | 65.354 | 127 | 42 | 1 | 119 | 245 | 219 | 343 | 1.37e-54 | 185 |
| MS.gene44879.t1 | MTR_7g037260 | 47.340 | 188 | 93 | 4 | 119 | 305 | 143 | 325 | 2.33e-53 | 180 |
| MS.gene44879.t1 | MTR_5g049190 | 50.000 | 184 | 74 | 3 | 119 | 284 | 17 | 200 | 1.15e-52 | 174 |
| MS.gene44879.t1 | MTR_7g451170 | 63.492 | 126 | 44 | 1 | 120 | 245 | 190 | 313 | 3.50e-52 | 178 |
| MS.gene44879.t1 | MTR_7g037130 | 44.615 | 195 | 88 | 4 | 119 | 305 | 143 | 325 | 1.14e-47 | 165 |
| MS.gene44879.t1 | MTR_1g057980 | 47.500 | 160 | 75 | 3 | 121 | 279 | 80 | 231 | 1.75e-43 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44879.t1 | AT5G58850 | 47.080 | 274 | 110 | 6 | 111 | 352 | 95 | 365 | 6.90e-68 | 219 |
| MS.gene44879.t1 | AT5G11050 | 46.570 | 277 | 108 | 7 | 110 | 352 | 94 | 364 | 8.59e-65 | 211 |
| MS.gene44879.t1 | AT4G18770 | 45.415 | 229 | 101 | 6 | 42 | 263 | 141 | 352 | 8.68e-60 | 198 |
| MS.gene44879.t1 | AT3G27785 | 63.448 | 145 | 48 | 2 | 109 | 252 | 177 | 317 | 2.03e-57 | 192 |
| MS.gene44879.t1 | AT5G40360 | 53.061 | 147 | 61 | 2 | 111 | 257 | 148 | 286 | 2.65e-48 | 167 |
| MS.gene44879.t1 | AT3G09370 | 61.538 | 104 | 40 | 0 | 119 | 222 | 133 | 236 | 3.19e-41 | 151 |
| MS.gene44879.t1 | AT3G09370 | 61.538 | 104 | 40 | 0 | 119 | 222 | 128 | 231 | 3.43e-41 | 151 |
| MS.gene44879.t1 | AT3G09370 | 45.513 | 156 | 71 | 2 | 119 | 261 | 141 | 295 | 3.83e-41 | 151 |
| MS.gene44879.t1 | AT5G02320 | 61.165 | 103 | 40 | 0 | 119 | 221 | 65 | 167 | 1.94e-40 | 149 |
| MS.gene44879.t1 | AT5G02320 | 61.165 | 103 | 40 | 0 | 119 | 221 | 125 | 227 | 2.12e-40 | 149 |
Find 76 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACCAACATTGTGGGA+AGG | 0.651290 | 5.2:-59279383 | MS.gene44879:CDS |
| AAATGTCAATGACCCTTTCA+TGG | 0.400644 | 5.2:-59279628 | MS.gene44879:CDS |
| AACTAAGAAAGAGACTTCCG+AGG | 0.693403 | 5.2:-59279358 | MS.gene44879:CDS |
| AACTTCCACATTGTGCCTTA+AGG | 0.380633 | 5.2:-59279424 | MS.gene44879:CDS |
| AAGAATGAAACTAATTCCAA+GGG | 0.598750 | 5.2:-59277904 | MS.gene44879:CDS |
| AAGCTTGATTCTTGAGTATT+TGG | 0.147400 | 5.2:+59279504 | None:intergenic |
| AAGTGTTGCATGAGAGGAAG+AGG | 0.420763 | 5.2:+59277804 | None:intergenic |
| AATATCCTTAAGGCACAATG+TGG | 0.654859 | 5.2:+59279419 | None:intergenic |
| ACATATTCAACAATTGAGAG+AGG | 0.676560 | 5.2:+59277551 | None:intergenic |
| ACTTCCGAGGTCTTGATCAA+AGG | 0.601817 | 5.2:-59279345 | MS.gene44879:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCTTGATTCTTGAGTATT+TGG | + | chr5.2:59277735-59277754 | None:intergenic | 30.0% | |
| ACATATTCAACAATTGAGAG+AGG | + | chr5.2:59279688-59279707 | None:intergenic | 30.0% | |
| AGGAAATTATTCATAGAGGT+TGG | + | chr5.2:59279650-59279669 | None:intergenic | 30.0% | |
| ATCTAGATCATCGGATAAAT+AGG | + | chr5.2:59278441-59278460 | None:intergenic | 30.0% | |
| CATAACCTTGAATATCCTTA+AGG | + | chr5.2:59277830-59277849 | None:intergenic | 30.0% | |
| CATAAGAATCTATCCATGAA+AGG | + | chr5.2:59277624-59277643 | None:intergenic | 30.0% | |
| CCAAATGCATGTTGTAATTT+GGG | + | chr5.2:59279622-59279641 | None:intergenic | 30.0% | |
| CTTAAGGATATTCAAGGTTA+TGG | - | chr5.2:59277828-59277847 | MS.gene44879:CDS | 30.0% | |
| CTTTCATGGATAGATTCTTA+TGG | - | chr5.2:59277622-59277641 | MS.gene44879:CDS | 30.0% | |
| GAAGAATGAAACTAATTCCA+AGG | - | chr5.2:59279331-59279350 | MS.gene44879:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 59277549 | 59279709 | 59277549 | ID=MS.gene44879 |
| chr5.2 | mRNA | 59277549 | 59279709 | 59277549 | ID=MS.gene44879.t1;Parent=MS.gene44879 |
| chr5.2 | exon | 59279321 | 59279709 | 59279321 | ID=MS.gene44879.t1.exon1;Parent=MS.gene44879.t1 |
| chr5.2 | CDS | 59279321 | 59279709 | 59279321 | ID=cds.MS.gene44879.t1;Parent=MS.gene44879.t1 |
| chr5.2 | exon | 59279009 | 59279135 | 59279009 | ID=MS.gene44879.t1.exon2;Parent=MS.gene44879.t1 |
| chr5.2 | CDS | 59279009 | 59279135 | 59279009 | ID=cds.MS.gene44879.t1;Parent=MS.gene44879.t1 |
| chr5.2 | exon | 59277549 | 59278094 | 59277549 | ID=MS.gene44879.t1.exon3;Parent=MS.gene44879.t1 |
| chr5.2 | CDS | 59277549 | 59278094 | 59277549 | ID=cds.MS.gene44879.t1;Parent=MS.gene44879.t1 |
| Gene Sequence |
>MS.gene44879 ATGGTAAGAGGAGGAAATCATGGGGTCAATGGCGTTAAAATCTTTGGCAATGAAAATATAAATGTCAATGACCCTTTCATGGATAGATTCTTATGGGGTCAAATGATTCACTACCCTAACCATCAACAAAACAATGCAAGTATGTTCCCTTACTCTTATGGTTCCAACCATGAGTTTATGTGGCCAAATACTCAAGAATCAAGCTTTGTTGTTGATGGTGTCTTAGCAAATGAAGAGGCTTTGAAGTGGACTAACTTCAACCAAACTTCCACATTGTGCCTTAAGGATATTCAAGGTTATGGTGAAAACACCAACATTGTGGGAAGGATAACTAAGAAAGAGACTTCCGAGGTCTTGATCAAAGGACAGTGGACAAACGAAGAAGACAGGTTAAATTCATATCTTATATCGGGTTTGAGTACCTCTTATATTCTTTTTTAATCGTATATCTATATATTTTGTAACTTTAAAAAAAAGGTTCTTTATATAGAAAAAGCTCCATTTTTTATAAAGAACTATGATTTGTACTAAACTTTTATTTATTAATGTGTTTTATTTGTTATGATGATTAAAGGAAACTGATAAAGTTGGTTAAGCAATATGGCGAAAGAAAATGGGCTCAAATAGCTGAGAAGTTAGAAGGAAGGGTTGGCAAGCAGTGTAGAGAGCGATGGCATAATCATTTGCGTCCTGATATTAAGGTTAGATATATATGTACCCATTTTTTGATTTCATATAATTTTTTTTTACAAGTTTTGTGCTTGAATTTGACCCTAGGATGATATAGTAGCACAACGATAAATTTTGCTTCTACATAGTTGCATGTAATTGATTTCTATTTAATTTCAGCAACTATGTTTTTCGAGATTATTCATTGAAATAATATTTTCCTATTTATCCGATGATCTAGATTTAGATTCATATTTTTCAAGGATCTGTGTCTTTTAGGGAGAATCTATGTTTGTTATCTGCATACATAGGTTGACTAGAGATCTAATTTCTTTGTTTTCTTTATAGTTTTTTTACAGTTATTTTCTTGTTAGTTTGTTTTCTTCTTATAGTTAGAACATATGTCTTGTTAATATAAATCATCAATCTTGATGTGGGCTTCATCCATTATTAATTTAGCACAAAATAAAAATGTAAAATTTTGTTCTAATCTACCCTTTGATAAATGATACTTTTTGTCTCTCAACAATTTTTTTGACAAAATTGTTCCTTAACATTTGAAATATGTCATTTTTGATTGGTGAAATTCAATTTTATTAATCAATTCAATTTCTTCCAAAATAAACAACATATTTTCAAGATACACAAAAACCATAAACAACATATTTCAAAGTAAGAGAACATCACTTTTCAAAAAATGTCTAAAACCAAATTTTAAACATTTTTAAGGACGAAAACATTCTTAACCTTACAGCTTTAGAACTTGGCATTGAACCTCATATGCAATACTTATATTTTTAATATTTTTTTCCTCTCCAAATAATTACATACATTTTTATTTACAGATTTTTAATTAGTTTAGTTTCAATAAAATTTTTTTAAGTAACTTTCAAAATTGTAATACTAATTATTTTACTTGGGAAACTTATAATTAATATTTTCACAGAAAGATAGTTGGAGTGAAGAAGAAGAGAAGATATTAGTAGCTACACATGCCAAAATTGGAAACCGTTGGGCTGAGATAGCAAAGAAGATTCCAGGAAGAACTGAAAATGCCATAAAAAATCATTGGAATGCCACTAAAAGGAGACAAAATTCAAGAAGAAAGAACAAGAAGAATGAAACTAATTCCAAGGGATCAAAACCTAAGTCCTATATACTTGAAAACTATATCAAAACCAACACCTCCATCACTACAAATATTACCAACATACCCTCTTCCTCTCATGCAACACTTTCCTTAAACTTTGAAGAAAATCAATCAAATCCTTTTTTCAATGAATTACCTTCTGAATCTTTTTCCAATGAATCACACTTCATACAACAAATTTTGTTGGATGATAATAATCAAAATGTTGTTGATGATGTGAATGAGAGTGAGCTTGTTCATTCAATCCAATACCCAAATTACAACATGCATTTGGATACTCCAACCTCTATGAATAATTTCCTTCCCTATGATCTTTACCTCTCTCAATTGTTGAATATGTAG |
| Protein sequence |
>MS.gene44879.t1 MVRGGNHGVNGVKIFGNENINVNDPFMDRFLWGQMIHYPNHQQNNASMFPYSYGSNHEFMWPNTQESSFVVDGVLANEEALKWTNFNQTSTLCLKDIQGYGENTNIVGRITKKETSEVLIKGQWTNEEDRKLIKLVKQYGERKWAQIAEKLEGRVGKQCRERWHNHLRPDIKKDSWSEEEEKILVATHAKIGNRWAEIAKKIPGRTENAIKNHWNATKRRQNSRRKNKKNETNSKGSKPKSYILENYIKTNTSITTNITNIPSSSHATLSLNFEENQSNPFFNELPSESFSNESHFIQQILLDDNNQNVVDDVNESELVHSIQYPNYNMHLDTPTSMNNFLPYDLYLSQLLNM |
