AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45087

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45087.t1 MTR_5g035040 79.592 392 56 1 12 403 9 376 0.0 605
MS.gene45087.t1 MTR_5g035060 74.459 231 54 2 178 403 69 299 7.04e-114 335
MS.gene45087.t1 MTR_5g035050 85.227 88 13 0 316 403 29 116 3.87e-47 157
MS.gene45087.t1 MTR_7g033775 72.059 136 35 2 4 137 14 148 1.64e-43 150
MS.gene45087.t1 MTR_4g105200 58.537 82 34 0 36 117 37 118 3.27e-25 102
MS.gene45087.t1 MTR_6g005040 46.847 111 58 1 21 131 45 154 3.51e-16 77.0
MS.gene45087.t1 MTR_3g109190 35.354 99 64 0 39 137 35 133 1.04e-14 71.6
MS.gene45087.t1 MTR_1g090250 42.500 80 46 0 38 117 31 110 1.63e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45087.t1 AT1G24040 60.538 223 81 2 176 391 80 302 1.14e-90 276
MS.gene45087.t1 AT1G24040 60.538 223 81 2 176 391 80 302 1.14e-90 276
MS.gene45087.t1 AT1G24040 60.538 223 81 2 176 391 80 302 1.14e-90 276
MS.gene45087.t1 AT3G01570 46.774 124 64 2 14 137 13 134 3.72e-29 112
MS.gene45087.t1 AT3G27660 50.467 107 53 0 31 137 43 149 1.77e-20 89.0
MS.gene45087.t1 AT3G27660 50.467 107 53 0 31 137 43 149 1.77e-20 89.0
MS.gene45087.t1 AT5G40420 44.444 135 61 1 17 137 17 151 2.58e-19 85.9
MS.gene45087.t1 AT5G51210 44.304 79 44 0 39 117 26 104 4.35e-17 78.2
MS.gene45087.t1 AT4G25140 39.000 100 56 1 17 116 25 119 4.32e-14 70.5

Find 95 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACCAAATCCGGTTCAATT+TGG 0.176975 5.3:-23972661 MS.gene45087:CDS
GTCATTGGCTTGGCTGTTGC+TGG 0.231751 5.3:-23972995 MS.gene45087:CDS
CTTGCGGGACTCTCCCTTCT+TGG 0.248133 5.3:-23973091 MS.gene45087:CDS
ACACATTGATATAACATTTC+GGG 0.269650 5.3:+23972561 None:intergenic
TTGAACCGGATTTGGTGTTT+TGG 0.274567 5.3:+23972666 None:intergenic
AACCCAAATTGAACCGGATT+TGG 0.287146 5.3:+23972658 None:intergenic
CACCAAATCCGGTTCAATTT+GGG 0.288605 5.3:-23972660 MS.gene45087:CDS
ATAAATGTAAGGCGAATCTT+TGG 0.316634 5.3:+23972361 None:intergenic
AACACATTGATATAACATTT+CGG 0.321848 5.3:+23972560 None:intergenic
CAAGTCCTTGCCGTAATTAC+CGG 0.324579 5.3:-23973142 MS.gene45087:CDS
GTTCCAGCCACCATTGTCAT+TGG 0.345015 5.3:-23973010 MS.gene45087:CDS
TGGCACTCTCCTTGTTCTCA+TGG 0.345702 5.3:-23972943 MS.gene45087:CDS
TCTGTAGAGACCCTTGATCC+TGG 0.345748 5.3:+23972903 None:intergenic
GGTGTACTGCTTGCACTTGC+GGG 0.361223 5.3:-23973106 MS.gene45087:CDS
TAATCAAATAATGTTTCATA+AGG 0.370814 5.3:+23972531 None:intergenic
CCGCATGTGGCGACACTGAT+TGG 0.372849 5.3:-23972491 MS.gene45087:CDS
AGCCACCATTGTCATTGGCT+TGG 0.389466 5.3:-23973005 MS.gene45087:CDS
AACAGGAAGGCCGGTAATTA+CGG 0.397877 5.3:+23973132 None:intergenic
GAGGACTCGACAAGTGGTTT+TGG 0.398205 5.3:+23972739 None:intergenic
ATGGTGGCTGGAACAAGAAC+AGG 0.405324 5.3:+23973019 None:intergenic
GTGTTGCAAGTTGGTTAGTT+CGG 0.409520 5.3:+23972709 None:intergenic
TTCGTCTCTGCAACGTCAGC+AGG 0.429890 5.3:+23971289 None:intergenic
CTGTAGAGACCCTTGATCCT+GGG 0.434813 5.3:+23972904 None:intergenic
TACTCGAACCCAAATTGAAC+CGG 0.435802 5.3:+23972652 None:intergenic
TTCGCCTTACATTTATAGCT+TGG 0.438162 5.3:-23972354 MS.gene45087:CDS
TACTCCCAAAACACCAAATC+CGG 0.443113 5.3:-23972671 MS.gene45087:CDS
ATTCTTTCACTCTCCTCCTT+AGG 0.453455 5.3:-23972788 MS.gene45087:CDS
CGCATGTGGCGACACTGATT+GGG 0.458513 5.3:-23972490 MS.gene45087:CDS
ATTCCCTTTAGGAGGGGCAT+TGG 0.469054 5.3:-23971462 MS.gene45087:intron
CAAGTGAGGAACTGATTTCT+CGG 0.471299 5.3:-23971422 MS.gene45087:CDS
GGCACTCTCCTTGTTCTCAT+GGG 0.476509 5.3:-23972942 MS.gene45087:CDS
CCGTGGATAAATCTCTTAGA+AGG 0.481490 5.3:-23972331 MS.gene45087:intron
GGGAGTAAAGGAAGGATTCG+AGG 0.486281 5.3:+23972687 None:intergenic
TAAATGTAAGGCGAATCTTT+GGG 0.489201 5.3:+23972362 None:intergenic
CACCTTCATAACGCTGTTGA+GGG 0.489611 5.3:+23973212 None:intergenic
GCAAGCAGTACACCACCAAC+AGG 0.500602 5.3:+23973115 None:intergenic
TCTTGGAACACTCACCGGAC+TGG 0.502163 5.3:-23973074 MS.gene45087:CDS
TCCCTTCTTGGAACACTCAC+CGG 0.503043 5.3:-23973079 MS.gene45087:CDS
TGGTGTACTGCTTGCACTTG+CGG 0.506312 5.3:-23973107 MS.gene45087:CDS
TGGGATTGTTACCGCCAGTC+CGG 0.516402 5.3:+23973060 None:intergenic
AATTACATACCCCAGGATCA+AGG 0.516935 5.3:-23972914 MS.gene45087:CDS
ATTTCTCGGATGAGTCCATC+AGG 0.518088 5.3:-23971408 MS.gene45087:CDS
ATTACCGGCCTTCCTGTTGG+TGG 0.520998 5.3:-23973127 MS.gene45087:CDS
ACGGCCAAGCTATAAATGTA+AGG 0.524650 5.3:+23972350 None:intergenic
GGCGAATCTTTGGGAGGAGT+GGG 0.529573 5.3:+23972371 None:intergenic
CAAGGACTTGAGAAGTTGAC+AGG 0.532516 5.3:+23973155 None:intergenic
CCACCACGCTGTTACGATAC+CGG 0.533282 5.3:-23973241 MS.gene45087:CDS
CAACAGCGTTATGAAGGTGG+TGG 0.536319 5.3:-23973208 MS.gene45087:CDS
TACACCACCAACAGGAAGGC+CGG 0.537821 5.3:+23973123 None:intergenic
ACCCCTCAACAGCGTTATGA+AGG 0.539761 5.3:-23973214 MS.gene45087:CDS
GCCATCAACCCAAAAGCTGC+CGG 0.540087 5.3:+23972962 None:intergenic
AAGCCAATGACAATGGTGGC+TGG 0.540091 5.3:+23973007 None:intergenic
CCACCTTCATAACGCTGTTG+AGG 0.540213 5.3:+23973211 None:intergenic
GTAATTACCGGCCTTCCTGT+TGG 0.540326 5.3:-23973130 MS.gene45087:CDS
TCCGGTGAGTGTTCCAAGAA+GGG 0.541690 5.3:+23973078 None:intergenic
TTACATTTATAGCTTGGCCG+TGG 0.542063 5.3:-23972348 MS.gene45087:CDS
GTCCGGTGAGTGTTCCAAGA+AGG 0.545489 5.3:+23973077 None:intergenic
TTTCTTCAGTTCTATTACAG+TGG 0.552035 5.3:-23972816 MS.gene45087:CDS
TGCAAGTAAACTTCTCCTGA+TGG 0.554907 5.3:+23971393 None:intergenic
AGGCGAATCTTTGGGAGGAG+TGG 0.555491 5.3:+23972370 None:intergenic
GGTGAAGAAATGCAGTTGGC+TGG 0.557318 5.3:-23972446 MS.gene45087:CDS
AACAGCGTTATGAAGGTGGT+GGG 0.557752 5.3:-23973207 MS.gene45087:CDS
GGATTCGAGGTGTTGCAAGT+TGG 0.564379 5.3:+23972700 None:intergenic
GGGCTGAAGAAGATGAAAAG+TGG 0.572089 5.3:+23973040 None:intergenic
TTTGAAGGCGTAGTTATGAG+AGG 0.572341 5.3:+23971243 None:intergenic
TGTAGAGACCCTTGATCCTG+GGG 0.582357 5.3:+23972905 None:intergenic
TCTACAGAAGCAATTCGAGT+TGG 0.583693 5.3:-23972888 MS.gene45087:CDS
GGCTGAAGAAGATGAAAAGT+GGG 0.586539 5.3:+23973041 None:intergenic
AACATTATTTGATTAAACGA+AGG 0.591213 5.3:-23972523 MS.gene45087:CDS
ATTCATGACCCATGAGAACA+AGG 0.593388 5.3:+23972934 None:intergenic
TGGTTAGTTCGGAGTTTGTG+AGG 0.593575 5.3:+23972720 None:intergenic
ATTACATACCCCAGGATCAA+GGG 0.596706 5.3:-23972913 MS.gene45087:CDS
TACTCATAGTCATCGGAACA+TGG 0.601957 5.3:+23971210 None:intergenic
CCTCAACAGCGTTATGAAGG+TGG 0.608029 5.3:-23973211 MS.gene45087:CDS
GGTCATGAATTACATACCCC+AGG 0.611661 5.3:-23972921 MS.gene45087:CDS
TGGAACACTCACCGGACTGG+CGG 0.613621 5.3:-23973071 MS.gene45087:CDS
TGTCTTCTACTCATAGTCAT+CGG 0.623600 5.3:+23971203 None:intergenic
GTTTGTGAGGACTCGACAAG+TGG 0.623742 5.3:+23972733 None:intergenic
CCAATCAGTGTCGCCACATG+CGG 0.627529 5.3:+23972491 None:intergenic
AACAGCCAAGCCAATGACAA+TGG 0.630398 5.3:+23973000 None:intergenic
AAGGACGTTGATGCCGCATG+TGG 0.632685 5.3:-23972504 MS.gene45087:CDS
GTGTATGGTGGAGTGGTGGT+CGG 0.635332 5.3:-23972858 MS.gene45087:CDS
GCAGTACACCACCAACAGGA+AGG 0.638216 5.3:+23973119 None:intergenic
CCTTCTAAGAGATTTATCCA+CGG 0.645724 5.3:+23972331 None:intergenic
TGATGGTGAAGAAATGCAGT+TGG 0.650400 5.3:-23972450 MS.gene45087:CDS
ACCTTCATAACGCTGTTGAG+GGG 0.651369 5.3:+23973213 None:intergenic
AACAGTGTATGGTGGAGTGG+TGG 0.660465 5.3:-23972862 MS.gene45087:CDS
ACAGCGTTATGAAGGTGGTG+GGG 0.661468 5.3:-23973206 MS.gene45087:CDS
GAAGGCCGGTAATTACGGCA+AGG 0.673386 5.3:+23973137 None:intergenic
AGCCAAGCCAATGACAATGG+TGG 0.680365 5.3:+23973003 None:intergenic
ACGCTGTTGAGGGGTAACAC+CGG 0.680408 5.3:+23973222 None:intergenic
ATGTAAGGCGAATCTTTGGG+AGG 0.683729 5.3:+23972365 None:intergenic
TGGTGGCTGGAACAAGAACA+GGG 0.708517 5.3:+23973020 None:intergenic
CCGGTATCGTAACAGCGTGG+TGG 0.714444 5.3:+23973241 None:intergenic
ACACCGGTATCGTAACAGCG+TGG 0.821558 5.3:+23973238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATAATGTTTCATA+AGG + chr5.3:23971937-23971956 None:intergenic 15.0%
!! AACACATTGATATAACATTT+CGG + chr5.3:23971908-23971927 None:intergenic 20.0%
!! AACATTATTTGATTAAACGA+AGG - chr5.3:23971942-23971961 MS.gene45087:intron 20.0%
!! AGCTTAATTATTTAAATTGC+AGG + chr5.3:23972827-23972846 None:intergenic 20.0%
!! TTTCGATTATTCAAAGATTA+AGG - chr5.3:23972314-23972333 MS.gene45087:intron 20.0%
!!! GAGATTTGTTTTGATAAAAA+TGG - chr5.3:23972049-23972068 MS.gene45087:intron 20.0%
! AATAAGCTAAAATCAGCTTA+TGG + chr5.3:23972443-23972462 None:intergenic 25.0%
! ACACATTGATATAACATTTC+GGG + chr5.3:23971907-23971926 None:intergenic 25.0%
! ATAAGAACTAATTTGACCAA+GGG - chr5.3:23972231-23972250 MS.gene45087:intron 25.0%
! GATTAAGTAGGATATTTCTT+AGG - chr5.3:23972692-23972711 MS.gene45087:CDS 25.0%
! GGTTACTTAAATCTTAAACT+TGG + chr5.3:23972851-23972870 None:intergenic 25.0%
! TATTCTTGGTCTAATTTGAT+TGG - chr5.3:23972260-23972279 MS.gene45087:intron 25.0%
! TCTGATATTTAATTCCCTTT+AGG - chr5.3:23972992-23973011 MS.gene45087:CDS 25.0%
!! ATATTTAATTCCCTTTAGGA+GGG - chr5.3:23972996-23973015 MS.gene45087:CDS 25.0%
!! CTACAAACTGAAACATTTTT+GGG - chr5.3:23972378-23972397 MS.gene45087:CDS 25.0%
!! GATATTTCTTAGGTGAATTT+TGG - chr5.3:23972702-23972721 MS.gene45087:CDS 25.0%
!! GCTTTTGTATACATGTTAAA+TGG + chr5.3:23973120-23973139 None:intergenic 25.0%
AAATGCAAATTTCAGCAACT+TGG + chr5.3:23972179-23972198 None:intergenic 30.0%
AGAAAGTTAAAACAGACACT+CGG + chr5.3:23972961-23972980 None:intergenic 30.0%
ATAAATGTAAGGCGAATCTT+TGG + chr5.3:23972107-23972126 None:intergenic 30.0%
ATAATAGCAACGAGAAAATG+TGG + chr5.3:23972527-23972546 None:intergenic 30.0%
CATAAGAACTAATTTGACCA+AGG - chr5.3:23972230-23972249 MS.gene45087:intron 30.0%
CCAAAAATGTTTCAGTTTGT+AGG + chr5.3:23972380-23972399 None:intergenic 30.0%
GATATTTAATTCCCTTTAGG+AGG - chr5.3:23972995-23973014 MS.gene45087:CDS 30.0%
GATTAAGGCTGTATTTAGAT+TGG - chr5.3:23972329-23972348 MS.gene45087:intron 30.0%
TAAATGTAAGGCGAATCTTT+GGG + chr5.3:23972106-23972125 None:intergenic 30.0%
TGCTGAAATTTGCATTTCTT+TGG - chr5.3:23972182-23972201 MS.gene45087:intron 30.0%
TGTGTTCATAAACTATCCTT+GGG + chr5.3:23972476-23972495 None:intergenic 30.0%
TGTTTGAAAATGTGAGCTAT+GGG + chr5.3:23972899-23972918 None:intergenic 30.0%
TTCACATCTAGATACACTTA+GGG + chr5.3:23972597-23972616 None:intergenic 30.0%
TTTCACATCTAGATACACTT+AGG + chr5.3:23972598-23972617 None:intergenic 30.0%
TTTCTTCAGTTCTATTACAG+TGG - chr5.3:23971649-23971668 MS.gene45087:intron 30.0%
! ATGTTGAAGCAATGATTTTG+AGG + chr5.3:23971704-23971723 None:intergenic 30.0%
! CCTACAAACTGAAACATTTT+TGG - chr5.3:23972377-23972396 MS.gene45087:CDS 30.0%
! CTTGGTCAAATTAGTTCTTA+TGG + chr5.3:23972232-23972251 None:intergenic 30.0%
! TATTTAATTCCCTTTAGGAG+GGG - chr5.3:23972997-23973016 MS.gene45087:CDS 30.0%
! TGTTGAAGCAATGATTTTGA+GGG + chr5.3:23971703-23971722 None:intergenic 30.0%
! TTTTATAGAGGAGTTACTGA+TGG - chr5.3:23971998-23972017 MS.gene45087:intron 30.0%
!! TCATCACTAGCAATTTTAGT+TGG - chr5.3:23972796-23972815 MS.gene45087:CDS 30.0%
!!! CATCACTAGCAATTTTAGTT+GGG - chr5.3:23972797-23972816 MS.gene45087:CDS 30.0%
AAAGCTAGAACTACTAGATG+CGG - chr5.3:23972615-23972634 MS.gene45087:CDS 35.0%
ACCAAGAATATGTATTCCCT+TGG + chr5.3:23972250-23972269 None:intergenic 35.0%
ACCAAGGGAATACATATTCT+TGG - chr5.3:23972246-23972265 MS.gene45087:intron 35.0%
ACTCTTCTGCTAGATTAAGT+AGG - chr5.3:23972680-23972699 MS.gene45087:CDS 35.0%
ATTGGCTATATGCATATCCA+GGG - chr5.3:23972278-23972297 MS.gene45087:intron 35.0%
CTGTGTTCATAAACTATCCT+TGG + chr5.3:23972477-23972496 None:intergenic 35.0%
GTGTTTGAAAATGTGAGCTA+TGG + chr5.3:23972900-23972919 None:intergenic 35.0%
TTCGCCTTACATTTATAGCT+TGG - chr5.3:23972111-23972130 MS.gene45087:intron 35.0%
! AGCTCACATTTTCAAACACA+TGG - chr5.3:23972901-23972920 MS.gene45087:CDS 35.0%
! CATTGGACGACATCTTTTAA+AGG - chr5.3:23973020-23973039 MS.gene45087:CDS 35.0%
! CCTTCTAAGAGATTTATCCA+CGG + chr5.3:23972137-23972156 None:intergenic 35.0%
! TCGCGACGAGAATATATTTT+CGG + chr5.3:23972872-23972891 None:intergenic 35.0%
!! ACACTGATTGGGTTTTATAG+AGG - chr5.3:23971986-23972005 MS.gene45087:intron 35.0%
!! ACATCTTTTAAAGGCAAGTG+AGG - chr5.3:23973029-23973048 MS.gene45087:CDS 35.0%
!!! ATTTGGTGTTTTGGGAGTAA+AGG + chr5.3:23971793-23971812 None:intergenic 35.0%
AACCCAAATTGAACCGGATT+TGG + chr5.3:23971810-23971829 None:intergenic 40.0%
AACTATCCTTGGGAGTTTGT+TGG + chr5.3:23972466-23972485 None:intergenic 40.0%
AAGCTAGAACTACTAGATGC+GGG - chr5.3:23972616-23972635 MS.gene45087:CDS 40.0%
AATTACATACCCCAGGATCA+AGG - chr5.3:23971551-23971570 MS.gene45087:intron 40.0%
ACACCAAATCCGGTTCAATT+TGG - chr5.3:23971804-23971823 MS.gene45087:intron 40.0%
ACGGCCAAGCTATAAATGTA+AGG + chr5.3:23972118-23972137 None:intergenic 40.0%
ACTACTAGATGCGGGTAAAA+TGG - chr5.3:23972624-23972643 MS.gene45087:CDS 40.0%
ATTACATACCCCAGGATCAA+GGG - chr5.3:23971552-23971571 MS.gene45087:intron 40.0%
ATTCATGACCCATGAGAACA+AGG + chr5.3:23971534-23971553 None:intergenic 40.0%
ATTCTTTCACTCTCCTCCTT+AGG - chr5.3:23971677-23971696 MS.gene45087:intron 40.0%
CACCAAATCCGGTTCAATTT+GGG - chr5.3:23971805-23971824 MS.gene45087:intron 40.0%
CCGTGGATAAATCTCTTAGA+AGG - chr5.3:23972134-23972153 MS.gene45087:intron 40.0%
CTTATTCCAACAAACTCCCA+AGG - chr5.3:23972457-23972476 MS.gene45087:CDS 40.0%
GGCTGAAGAAGATGAAAAGT+GGG + chr5.3:23971427-23971446 None:intergenic 40.0%
GTTGGAAAAACAGTGTATGG+TGG - chr5.3:23971595-23971614 MS.gene45087:intron 40.0%
TACTCATAGTCATCGGAACA+TGG + chr5.3:23973258-23973277 None:intergenic 40.0%
TACTCCCAAAACACCAAATC+CGG - chr5.3:23971794-23971813 MS.gene45087:intron 40.0%
TACTCGAACCCAAATTGAAC+CGG + chr5.3:23971816-23971835 None:intergenic 40.0%
TCTACAGAAGCAATTCGAGT+TGG - chr5.3:23971577-23971596 MS.gene45087:intron 40.0%
TGCAAGTAAACTTCTCCTGA+TGG + chr5.3:23973075-23973094 None:intergenic 40.0%
TTACATTTATAGCTTGGCCG+TGG - chr5.3:23972117-23972136 MS.gene45087:intron 40.0%
TTGGCTATATGCATATCCAG+GGG - chr5.3:23972279-23972298 MS.gene45087:intron 40.0%
! AAAAACAGTGTATGGTGGAG+TGG - chr5.3:23971600-23971619 MS.gene45087:intron 40.0%
! CAAGTGAGGAACTGATTTCT+CGG - chr5.3:23973043-23973062 MS.gene45087:CDS 40.0%
! CGAGTTGGAAAAACAGTGTA+TGG - chr5.3:23971592-23971611 MS.gene45087:intron 40.0%
! GATTGGCTATATGCATATCC+AGG - chr5.3:23972277-23972296 MS.gene45087:intron 40.0%
! TGATGGTGAAGAAATGCAGT+TGG - chr5.3:23972015-23972034 MS.gene45087:intron 40.0%
! TTTGAAGGCGTAGTTATGAG+AGG + chr5.3:23973225-23973244 None:intergenic 40.0%
!! GTGTTGCAAGTTGGTTAGTT+CGG + chr5.3:23971759-23971778 None:intergenic 40.0%
!!! TGAACCGGATTTGGTGTTTT+GGG + chr5.3:23971801-23971820 None:intergenic 40.0%
!!! TTGAACCGGATTTGGTGTTT+TGG + chr5.3:23971802-23971821 None:intergenic 40.0%
AACAGCCAAGCCAATGACAA+TGG + chr5.3:23971468-23971487 None:intergenic 45.0%
AACAGCGTTATGAAGGTGGT+GGG - chr5.3:23971258-23971277 MS.gene45087:CDS 45.0%
AACAGGAAGGCCGGTAATTA+CGG + chr5.3:23971336-23971355 None:intergenic 45.0%
ACCTTCATAACGCTGTTGAG+GGG + chr5.3:23971255-23971274 None:intergenic 45.0%
ATGTAAGGCGAATCTTTGGG+AGG + chr5.3:23972103-23972122 None:intergenic 45.0%
ATTTCTCGGATGAGTCCATC+AGG - chr5.3:23973057-23973076 MS.gene45087:CDS 45.0%
CAAGGACTTGAGAAGTTGAC+AGG + chr5.3:23971313-23971332 None:intergenic 45.0%
CAAGTCCTTGCCGTAATTAC+CGG - chr5.3:23971323-23971342 MS.gene45087:CDS 45.0%
CACCTTCATAACGCTGTTGA+GGG + chr5.3:23971256-23971275 None:intergenic 45.0%
GAAACTACACATTTAGCCCC+TGG + chr5.3:23972298-23972317 None:intergenic 45.0%
GGGCTGAAGAAGATGAAAAG+TGG + chr5.3:23971428-23971447 None:intergenic 45.0%
GGTCATGAATTACATACCCC+AGG - chr5.3:23971544-23971563 MS.gene45087:intron 45.0%
! CATGGCACGTCTGATTTTGA+AGG + chr5.3:23973240-23973259 None:intergenic 45.0%
! TGGTTAGTTCGGAGTTTGTG+AGG + chr5.3:23971748-23971767 None:intergenic 45.0%
!! ATGATTTTGAGGGCCTAAGG+AGG + chr5.3:23971693-23971712 None:intergenic 45.0%
!! GCAATGATTTTGAGGGCCTA+AGG + chr5.3:23971696-23971715 None:intergenic 45.0%
!!! GGTGTTTTGGGAGTAAAGGA+AGG + chr5.3:23971789-23971808 None:intergenic 45.0%
AAGCCAATGACAATGGTGGC+TGG + chr5.3:23971461-23971480 None:intergenic 50.0%
ACAGCGTTATGAAGGTGGTG+GGG - chr5.3:23971259-23971278 MS.gene45087:CDS 50.0%
ACCCCTCAACAGCGTTATGA+AGG - chr5.3:23971251-23971270 MS.gene45087:CDS 50.0%
AGCCAAGCCAATGACAATGG+TGG + chr5.3:23971465-23971484 None:intergenic 50.0%
ATGGTGGCTGGAACAAGAAC+AGG + chr5.3:23971449-23971468 None:intergenic 50.0%
CAACAGCGTTATGAAGGTGG+TGG - chr5.3:23971257-23971276 MS.gene45087:CDS 50.0%
CCACCTTCATAACGCTGTTG+AGG + chr5.3:23971257-23971276 None:intergenic 50.0%
CCTCAACAGCGTTATGAAGG+TGG - chr5.3:23971254-23971273 MS.gene45087:CDS 50.0%
CTGTAGAGACCCTTGATCCT+GGG + chr5.3:23971564-23971583 None:intergenic 50.0%
GGCACTCTCCTTGTTCTCAT+GGG - chr5.3:23971523-23971542 MS.gene45087:intron 50.0%
GGGAGTAAAGGAAGGATTCG+AGG + chr5.3:23971781-23971800 None:intergenic 50.0%
GGTGAAGAAATGCAGTTGGC+TGG - chr5.3:23972019-23972038 MS.gene45087:intron 50.0%
GTAATTACCGGCCTTCCTGT+TGG - chr5.3:23971335-23971354 MS.gene45087:CDS 50.0%
GTTCCAGCCACCATTGTCAT+TGG - chr5.3:23971455-23971474 MS.gene45087:intron 50.0%
GTTTGTGAGGACTCGACAAG+TGG + chr5.3:23971735-23971754 None:intergenic 50.0%
TCCCTTCTTGGAACACTCAC+CGG - chr5.3:23971386-23971405 MS.gene45087:CDS 50.0%
TCCGGTGAGTGTTCCAAGAA+GGG + chr5.3:23971390-23971409 None:intergenic 50.0%
TCTGTAGAGACCCTTGATCC+TGG + chr5.3:23971565-23971584 None:intergenic 50.0%
TGGCACTCTCCTTGTTCTCA+TGG - chr5.3:23971522-23971541 MS.gene45087:intron 50.0%
TGGTGGCTGGAACAAGAACA+GGG + chr5.3:23971448-23971467 None:intergenic 50.0%
TGGTGTACTGCTTGCACTTG+CGG - chr5.3:23971358-23971377 MS.gene45087:CDS 50.0%
TGTAGAGACCCTTGATCCTG+GGG + chr5.3:23971563-23971582 None:intergenic 50.0%
! AACAGTGTATGGTGGAGTGG+TGG - chr5.3:23971603-23971622 MS.gene45087:intron 50.0%
! AGCCACCATTGTCATTGGCT+TGG - chr5.3:23971460-23971479 MS.gene45087:intron 50.0%
! ATTCCCTTTAGGAGGGGCAT+TGG - chr5.3:23973003-23973022 MS.gene45087:CDS 50.0%
! GAGGACTCGACAAGTGGTTT+TGG + chr5.3:23971729-23971748 None:intergenic 50.0%
!! GGATTCGAGGTGTTGCAAGT+TGG + chr5.3:23971768-23971787 None:intergenic 50.0%
!!! CTTGGCTGTTGCTGGCTTTT+TGG - chr5.3:23971478-23971497 MS.gene45087:intron 50.0%
!!! TGTTGCTGGCTTTTTGGCAC+CGG - chr5.3:23971484-23971503 MS.gene45087:intron 50.0%
!!! TTTTGGCACCGGCAGCTTTT+GGG - chr5.3:23971495-23971514 MS.gene45087:intron 50.0%
!!! TTTTTGGCACCGGCAGCTTT+TGG - chr5.3:23971494-23971513 MS.gene45087:intron 50.0%
AAGGACGTTGATGCCGCATG+TGG - chr5.3:23971961-23971980 MS.gene45087:intron 55.0%
ACACCGGTATCGTAACAGCG+TGG + chr5.3:23971230-23971249 None:intergenic 55.0%
ACGCTGTTGAGGGGTAACAC+CGG + chr5.3:23971246-23971265 None:intergenic 55.0%
ATTACCGGCCTTCCTGTTGG+TGG - chr5.3:23971338-23971357 MS.gene45087:CDS 55.0%
CCAATCAGTGTCGCCACATG+CGG + chr5.3:23971977-23971996 None:intergenic 55.0%
CCACCACGCTGTTACGATAC+CGG - chr5.3:23971224-23971243 MS.gene45087:CDS 55.0%
CGTCCAATGCCCCTCCTAAA+GGG + chr5.3:23973009-23973028 None:intergenic 55.0%
GAAGGCCGGTAATTACGGCA+AGG + chr5.3:23971331-23971350 None:intergenic 55.0%
GCAAGCAGTACACCACCAAC+AGG + chr5.3:23971353-23971372 None:intergenic 55.0%
GCAGTACACCACCAACAGGA+AGG + chr5.3:23971349-23971368 None:intergenic 55.0%
GCCATCAACCCAAAAGCTGC+CGG + chr5.3:23971506-23971525 None:intergenic 55.0%
GGTGTACTGCTTGCACTTGC+GGG - chr5.3:23971359-23971378 MS.gene45087:CDS 55.0%
GTCCGGTGAGTGTTCCAAGA+AGG + chr5.3:23971391-23971410 None:intergenic 55.0%
TACACCACCAACAGGAAGGC+CGG + chr5.3:23971345-23971364 None:intergenic 55.0%
TCGTCCAATGCCCCTCCTAA+AGG + chr5.3:23973010-23973029 None:intergenic 55.0%
TCTTGGAACACTCACCGGAC+TGG - chr5.3:23971391-23971410 MS.gene45087:CDS 55.0%
TGGGATTGTTACCGCCAGTC+CGG + chr5.3:23971408-23971427 None:intergenic 55.0%
TTCGTCTCTGCAACGTCAGC+AGG + chr5.3:23973179-23973198 None:intergenic 55.0%
! AGGCGAATCTTTGGGAGGAG+TGG + chr5.3:23972098-23972117 None:intergenic 55.0%
! CGCATGTGGCGACACTGATT+GGG - chr5.3:23971975-23971994 MS.gene45087:intron 55.0%
! GGCGAATCTTTGGGAGGAGT+GGG + chr5.3:23972097-23972116 None:intergenic 55.0%
! GTGTATGGTGGAGTGGTGGT+CGG - chr5.3:23971607-23971626 MS.gene45087:intron 55.0%
!! GTCATTGGCTTGGCTGTTGC+TGG - chr5.3:23971470-23971489 MS.gene45087:intron 55.0%
!!! ACCGGCAGCTTTTGGGTTGA+TGG - chr5.3:23971502-23971521 MS.gene45087:intron 55.0%
CCGCATGTGGCGACACTGAT+TGG - chr5.3:23971974-23971993 MS.gene45087:intron 60.0%
CCGGTATCGTAACAGCGTGG+TGG + chr5.3:23971227-23971246 None:intergenic 60.0%
CTTGCGGGACTCTCCCTTCT+TGG - chr5.3:23971374-23971393 MS.gene45087:CDS 60.0%
! TGGAACACTCACCGGACTGG+CGG - chr5.3:23971394-23971413 MS.gene45087:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 23971209 23973278 23971209 ID=MS.gene45087
chr5.3 mRNA 23971209 23973278 23971209 ID=MS.gene45087.t1;Parent=MS.gene45087
chr5.3 exon 23972332 23973278 23972332 ID=MS.gene45087.t1.exon1;Parent=MS.gene45087.t1
chr5.3 CDS 23972332 23973278 23972332 ID=cds.MS.gene45087.t1;Parent=MS.gene45087.t1
chr5.3 exon 23971209 23971473 23971209 ID=MS.gene45087.t1.exon2;Parent=MS.gene45087.t1
chr5.3 CDS 23971209 23971473 23971209 ID=cds.MS.gene45087.t1;Parent=MS.gene45087.t1
Gene Sequence

>MS.gene45087

ATGTCAGAACGTACACCACCACGCTGTTACGATACCGGTGTTACCCCTCAACAGCGTTATGAAGGTGGTGGGGTTACTACTCACTACTCTGAGAAAAGAAGCCTGTCAACTTCTCAAGTCCTTGCCGTAATTACCGGCCTTCCTGTTGGTGGTGTACTGCTTGCACTTGCGGGACTCTCCCTTCTTGGAACACTCACCGGACTGGCGGTAACAATCCCACTTTTCATCTTCTTCAGCCCTGTTCTTGTTCCAGCCACCATTGTCATTGGCTTGGCTGTTGCTGGCTTTTTGGCACCGGCAGCTTTTGGGTTGATGGCACTCTCCTTGTTCTCATGGGTCATGAATTACATACCCCAGGATCAAGGGTCTCTACAGAAGCAATTCGAGTTGGAAAAACAGTGTATGGTGGAGTGGTGGTCGGAGTGCTGTAACATGAAAGTTTTCTTCAGTTCTATTACAGTGGCAGCAATTCTTTCACTCTCCTCCTTAGGCCCTCAAAATCATTGCTTCAACATCACCAAAACCACTTGTCGAGTCCTCACAAACTCCGAACTAACCAACTTGCAACACCTCGAATCCTTCCTTTACTCCCAAAACACCAAATCCGGTTCAATTTGGGTTCGAGTAATGAGAGACAACGAAATTGACACAATAGTCTGTTTACTCGCAGATGCGTTCGCTGAATTGATGTTGTTCCCGAAATGTTATATCAATGTGTTGAGATTCCTTATGAAACATTATTTGATTAAACGAAGGACGTTGATGCCGCATGTGGCGACACTGATTGGGTTTTATAGAGGAGTTACTGATGGTGAAGAAATGCAGTTGGCTGGAACTGTTGAGATTTGTTTTGATAAAAATGGTGCTAATTCTTCTCGTGCTTCGCCCACTCCTCCCAAAGATTCGCCTTACATTTATAGCTTGGCCGTGGATAAATCTCTTAGAAGGTAAACATTATTTACTGTGTCCAAGTTGCTGAAATTTGCATTTCTTTGGTAAAATTAGCTCAAGTGTCATGAACCATAAGAACTAATTTGACCAAGGGAATACATATTCTTGGTCTAATTTGATTGGCTATATGCATATCCAGGGGCTAAATGTGTAGTTTCGATTATTCAAAGATTAAGGCTGTATTTAGATTGGTTTATGAATAACTTATTTGAGCTTACCTACAAACTGAAACATTTTTGGGTGTGTTTGTTAGAGCTTATGAAAATAGTTCAAAACATGTCCATAAGCTGATTTTAGCTTATTCCAACAAACTCCCAAGGATAGTTTATGAACACAGTTTGCAAATTATATGAAAACAGCTTAACCACATTTTCTCGTTGCTATTATAAGTAGCTTATACATAAGCGCTAAATTAAGTTGTTTATTCAAACATACCCTAAGTGTATCTAGATGTGAAAGCTAGAACTACTAGATGCGGGTAAAATGGATATTCAGAAACTAGTTTGATTATTAATTGTTAACTCTTCTGCTAGATTAAGTAGGATATTTCTTAGGTGAATTTTGGAGACAGTCTTGTCAGTATCATATACTTACTATGCTTTTAACTTTTCTGATCTTACAGCAAGATAAATATTTTCATCACTAGCAATTTTAGTTGGGAAAACCTGCAATTTAAATAATTAAGCTACCAAGTTTAAGATTTAAGTAACCGAAAATATATTCTCGTCGCGAAATTCCCATAGCTCACATTTTCAAACACATGGTATGTCTTCTCATTAAGCAAAAGAAAAAACGAAACCGAGTGTCTGTTTTAACTTTCTAAAAATGTCTTTCTGATATTTAATTCCCTTTAGGAGGGGCATTGGACGACATCTTTTAAAGGCAAGTGAGGAACTGATTTCTCGGATGAGTCCATCAGGAGAAGTTTACTTGCATTGCAGAATGATTGATGAAGCTCCATTTAACATGTATACAAAAGCTGATTACAAAATTGTTACATCAGATAGTATATTAGTCCTGCTGACGTTGCAGAGACGAAAATACTTGATGTGCAAGAAACTTCCTCTCATAACTACGCCTTCAAAATCAGACGTGCCATGTTCCGATGACTATGAGTAG
Protein sequence

>MS.gene45087.t1

MSERTPPRCYDTGVTPQQRYEGGGVTTHYSEKRSLSTSQVLAVITGLPVGGVLLALAGLSLLGTLTGLAVTIPLFIFFSPVLVPATIVIGLAVAGFLAPAAFGLMALSLFSWVMNYIPQDQGSLQKQFELEKQCMVEWWSECCNMKVFFSSITVAAILSLSSLGPQNHCFNITKTTCRVLTNSELTNLQHLESFLYSQNTKSGSIWVRVMRDNEIDTIVCLLADAFAELMLFPKCYINVLRFLMKHYLIKRRTLMPHVATLIGFYRGVTDGEEMQLAGTVEICFDKNGANSSRASPTPPKDSPYIYSLAVDKSLRRRGIGRHLLKASEELISRMSPSGEVYLHCRMIDEAPFNMYTKADYKIVTSDSILVLLTLQRRKYLMCKKLPLITTPSKSDVPCSDDYE