AlfalfaGEDB | MS.gene45174

M. sativa cultivar XinJiangDaYe / MS.gene45174

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45174.t1	AES96405.2	67.5	80	26	0	1	80	6	85	4.20E-22	113.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45174.t1	Q9XIK6	41.8	67	39	0	11	77	164	230	6.4e-11	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45174.t1	G7K6X3	67.5	80	26	0	1	80	6	85	3.0e-22	113.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene45174.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45174.t1	MTR_5g036790	67.500	80	26	0	1	80	6	85	1.83e-35	115
MS.gene45174.t1	MTR_5g036820	62.025	79	28	1	1	77	6	84	4.47e-29	99.4
MS.gene45174.t1	MTR_7g013525	48.148	81	41	1	1	80	1	81	4.40e-16	66.6
MS.gene45174.t1	MTR_7g013410	45.588	68	37	0	10	77	74	141	5.49e-15	65.1
MS.gene45174.t1	MTR_6g008970	41.429	70	41	0	8	77	142	211	1.09e-14	67.4
MS.gene45174.t1	MTR_6g008995	41.429	70	41	0	11	80	117	186	1.21e-14	67.4
MS.gene45174.t1	MTR_6g008995	41.429	70	41	0	11	80	60	129	1.23e-14	67.0
MS.gene45174.t1	MTR_5g036670	42.029	69	40	0	11	79	125	193	1.87e-13	63.9
MS.gene45174.t1	MTR_8g062700	40.299	67	40	0	11	77	124	190	3.27e-11	57.4
MS.gene45174.t1	MTR_8g062700	40.299	67	40	0	11	77	124	190	3.27e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45174.t1	AT1G10450	41.791	67	39	0	11	77	164	230	5.03e-15	68.6
MS.gene45174.t1	AT1G10450	41.791	67	39	0	11	77	113	179	5.22e-15	68.6
MS.gene45174.t1	AT1G10450	41.791	67	39	0	11	77	113	179	5.22e-15	68.6
MS.gene45174.t1	AT3G01320	40.000	70	42	0	8	77	135	204	1.79e-13	63.9
MS.gene45174.t1	AT3G01320	40.000	70	42	0	8	77	135	204	1.86e-13	63.9
MS.gene45174.t1	AT5G15020	41.429	70	41	0	8	77	129	198	6.57e-13	62.4
MS.gene45174.t1	AT5G15020	41.429	70	41	0	8	77	129	198	6.76e-13	62.4
MS.gene45174.t1	AT1G70060	41.667	72	42	0	6	77	92	163	8.30e-13	62.0
MS.gene45174.t1	AT1G70060	41.667	72	42	0	6	77	92	163	8.30e-13	62.0
MS.gene45174.t1	AT1G24190	43.284	67	38	0	11	77	96	162	1.49e-12	61.2
MS.gene45174.t1	AT1G24190	43.284	67	38	0	11	77	96	162	1.58e-12	61.2
MS.gene45174.t1	AT1G24190	43.284	67	38	0	11	77	96	162	1.60e-12	61.2
MS.gene45174.t1	AT1G59890	37.313	67	42	0	11	77	125	191	5.55e-12	59.7
MS.gene45174.t1	AT1G59890	37.313	67	42	0	11	77	125	191	5.60e-12	59.7
MS.gene45174.t1	AT1G59890	37.313	67	42	0	11	77	125	191	5.77e-12	59.7
MS.gene45174.t1	AT1G59890	37.313	67	42	0	11	77	125	191	5.77e-12	59.7
MS.gene45174.t1	AT1G70030	36.000	75	47	1	6	80	87	160	8.34e-11	54.7

Find 18 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGTGGTGGATTATGAATA+TGG	0.290334	5.1:+27319755	MS.gene45174:CDS
AGAGTCTTGATGATGTCATT+TGG	0.357491	5.1:+27319985	MS.gene45174:CDS
GAGTCTTGATGATGTCATTT+GGG	0.414208	5.1:+27319986	MS.gene45174:CDS
GGCCATGATGATCTTATTGA+TGG	0.460349	5.1:+27320110	MS.gene45174:CDS
GCCATGATGATCTTATTGAT+GGG	0.565009	5.1:+27320111	MS.gene45174:CDS
GAGATATATGATGAAGGTGA+AGG	0.585513	5.1:+27319781	MS.gene45174:CDS
TTTATTGATTTCCTTCCGCA+AGG	0.586502	5.1:+27320134	MS.gene45174:CDS
ATTCATAATCCACCACTGGC+CGG	0.587375	5.1:-27319751	None:intergenic
TGAAAAGAACATATGCTGAC+CGG	0.588434	5.1:+27319732	MS.gene45174:CDS
ATATGCTGACCGGCCAGTGG+TGG	0.590440	5.1:+27319742	MS.gene45174:CDS
ATCACTAGCCACCTTGCGGA+AGG	0.593474	5.1:-27320145	None:intergenic
ACCCATCAATAAGATCATCA+TGG	0.602513	5.1:-27320112	None:intergenic
CCATATTCATAATCCACCAC+TGG	0.614448	5.1:-27319755	None:intergenic
AACATATGCTGACCGGCCAG+TGG	0.637326	5.1:+27319739	MS.gene45174:CDS
ATTGATTTCCTTCCGCAAGG+TGG	0.643769	5.1:+27320137	MS.gene45174:CDS
TGGTTTGAGATATATGATGA+AGG	0.650047	5.1:+27319775	MS.gene45174:CDS
ATATATCACTAGCCACCTTG+CGG	0.662180	5.1:-27320149	None:intergenic
TCTTGATGATGTCATTTGGG+CGG	0.663888	5.1:+27319989	MS.gene45174:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTATGGTTTTAATTCTTC+AGG	+	chr5.1:27320070-27320089	MS.gene45174:intron	20.0%
!	AAGAGGAAAGAAAAATAAAG+AGG	-	chr5.1:27320034-27320053	None:intergenic	25.0%
!!!	TGGGATACTCTTTTTAATTA+TGG	+	chr5.1:27320055-27320074	MS.gene45174:intron	25.0%
!!!	TTATTTTTCTTTCCTCTTGT+GGG	+	chr5.1:27320036-27320055	MS.gene45174:intron	25.0%
!!!	TTTATTTTTCTTTCCTCTTG+TGG	+	chr5.1:27320035-27320054	MS.gene45174:intron	25.0%
	GGTGAAGGTAAGTTATATTA+TGG	+	chr5.1:27319796-27319815	MS.gene45174:intron	30.0%
!	TGGTTTGAGATATATGATGA+AGG	+	chr5.1:27319775-27319794	MS.gene45174:CDS	30.0%
!!!	CAGGTTTCTTTACTTTTTGA+AGG	+	chr5.1:27320089-27320108	MS.gene45174:intron	30.0%
	ACCCATCAATAAGATCATCA+TGG	-	chr5.1:27320115-27320134	None:intergenic	35.0%
	GAGATATATGATGAAGGTGA+AGG	+	chr5.1:27319781-27319800	MS.gene45174:CDS	35.0%
	GCCATGATGATCTTATTGAT+GGG	+	chr5.1:27320111-27320130	MS.gene45174:CDS	35.0%
	TGAAAAGAACATATGCTGAC+CGG	+	chr5.1:27319732-27319751	MS.gene45174:CDS	35.0%
!!	AGAGTCTTGATGATGTCATT+TGG	+	chr5.1:27319985-27320004	MS.gene45174:CDS	35.0%
!!	GAGTCTTGATGATGTCATTT+GGG	+	chr5.1:27319986-27320005	MS.gene45174:CDS	35.0%
!!	TAAAAAGAGTATCCCACAAG+AGG	-	chr5.1:27320051-27320070	None:intergenic	35.0%
!!	TTTATTGATTTCCTTCCGCA+AGG	+	chr5.1:27320134-27320153	MS.gene45174:CDS	35.0%
	CCATATTCATAATCCACCAC+TGG	-	chr5.1:27319758-27319777	None:intergenic	40.0%
	GGCCATGATGATCTTATTGA+TGG	+	chr5.1:27320110-27320129	MS.gene45174:CDS	40.0%
!!	CCAGTGGTGGATTATGAATA+TGG	+	chr5.1:27319755-27319774	MS.gene45174:CDS	40.0%
!!	TCTTGATGATGTCATTTGGG+CGG	+	chr5.1:27319989-27320008	MS.gene45174:CDS	40.0%
	ATTCATAATCCACCACTGGC+CGG	-	chr5.1:27319754-27319773	None:intergenic	45.0%
!	ATTGATTTCCTTCCGCAAGG+TGG	+	chr5.1:27320137-27320156	MS.gene45174:CDS	45.0%
	AACATATGCTGACCGGCCAG+TGG	+	chr5.1:27319739-27319758	MS.gene45174:CDS	55.0%
	ATATGCTGACCGGCCAGTGG+TGG	+	chr5.1:27319742-27319761	MS.gene45174:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	27319731	27320163	27319731	ID=MS.gene45174
chr5.1	mRNA	27319731	27320163	27319731	ID=MS.gene45174.t1;Parent=MS.gene45174
chr5.1	exon	27319731	27319802	27319731	ID=MS.gene45174.t1.exon1;Parent=MS.gene45174.t1
chr5.1	CDS	27319731	27319802	27319731	ID=cds.MS.gene45174.t1;Parent=MS.gene45174.t1
chr5.1	exon	27319912	27320010	27319912	ID=MS.gene45174.t1.exon2;Parent=MS.gene45174.t1
chr5.1	CDS	27319912	27320010	27319912	ID=cds.MS.gene45174.t1;Parent=MS.gene45174.t1
chr5.1	exon	27320092	27320163	27320092	ID=MS.gene45174.t1.exon3;Parent=MS.gene45174.t1
chr5.1	CDS	27320092	27320163	27320092	ID=cds.MS.gene45174.t1;Parent=MS.gene45174.t1

Gene Sequence

>MS.gene45174

ATGAAAAGAACATATGCTGACCGGCCAGTGGTGGATTATGAATATGGTTTGAGATATATGATGAAGGTGAAGGTAAGTTATATTATGGATTCGATTCTTTCACACATATATGTTTTCAATGTAGAATTAATCAATGAAATATTTCAGTTTTGACAAGTCTAATAGATGTTTTTTCATGCAGACTCGATTTCAAGATGCTCGTCATGTGTATTCGTCGTTTCAAGACATGCTGAAAATGTACAAAGAAAGAGAAAAGAGTCTTGATGATGTCATTTGGGCGGTATATGCTGAAAATATATGCCTCTTTATTTTTCTTTCCTCTTGTGGGATACTCTTTTTAATTATGGTTTTAATTCTTCAGGTTTCTTTACTTTTTGAAGGCCATGATGATCTTATTGATGGGTTTATTGATTTCCTTCCGCAAGGTGGCTAG

Protein sequence

>MS.gene45174.t1

MKRTYADRPVVDYEYGLRYMMKVKTRFQDARHVYSSFQDMLKMYKEREKSLDDVIWAVSLLFEGHDDLIDGFIDFLPQGG