Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45330.t1 | KEH29599.1 | 82.6 | 264 | 29 | 2 | 1 | 258 | 8 | 260 | 1.80E-98 | 369 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45330.t1 | A0A072UIK6 | 82.6 | 264 | 29 | 2 | 1 | 258 | 8 | 260 | 1.3e-98 | 369.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene45330.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45330.t1 | MTR_4g045977 | 85.227 | 264 | 22 | 3 | 1 | 258 | 8 | 260 | 3.28e-159 | 447 |
| MS.gene45330.t1 | MTR_4g107170 | 53.053 | 262 | 97 | 6 | 1 | 259 | 8 | 246 | 2.97e-82 | 247 |
| MS.gene45330.t1 | MTR_2g049610 | 43.290 | 231 | 126 | 3 | 1 | 229 | 8 | 235 | 1.35e-61 | 194 |
| MS.gene45330.t1 | MTR_1g115290 | 55.882 | 170 | 54 | 4 | 31 | 198 | 2 | 152 | 3.32e-56 | 177 |
| MS.gene45330.t1 | MTR_2g049630 | 55.280 | 161 | 69 | 3 | 1 | 159 | 10 | 169 | 9.32e-52 | 172 |
| MS.gene45330.t1 | MTR_2g049530 | 50.299 | 167 | 80 | 3 | 1 | 165 | 10 | 175 | 1.69e-49 | 161 |
| MS.gene45330.t1 | MTR_6g005440 | 35.829 | 187 | 106 | 3 | 1 | 183 | 8 | 184 | 6.51e-33 | 122 |
| MS.gene45330.t1 | MTR_6g018920 | 32.796 | 186 | 122 | 1 | 1 | 183 | 8 | 193 | 4.79e-29 | 112 |
| MS.gene45330.t1 | MTR_1g114730 | 34.021 | 194 | 124 | 2 | 1 | 191 | 8 | 200 | 1.38e-28 | 111 |
| MS.gene45330.t1 | MTR_6g005450 | 35.583 | 163 | 102 | 1 | 1 | 160 | 8 | 170 | 9.23e-27 | 106 |
| MS.gene45330.t1 | MTR_2g016210 | 25.503 | 149 | 101 | 2 | 1 | 141 | 8 | 154 | 3.29e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45330.t1 | AT5G55690 | 31.098 | 164 | 97 | 4 | 1 | 160 | 8 | 159 | 4.17e-13 | 67.8 |
| MS.gene45330.t1 | AT5G55690 | 31.098 | 164 | 97 | 4 | 1 | 160 | 8 | 159 | 4.17e-13 | 67.8 |
Find 37 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCAGATTTCCTTAATA+TGG | 0.236107 | 4.3:+53944915 | MS.gene45330:CDS |
| TTTACAATTGTAGGGTCTTC+TGG | 0.267825 | 4.3:-53944742 | None:intergenic |
| TTGTGGACATCAAATATTTC+AGG | 0.290143 | 4.3:-53944808 | None:intergenic |
| CAATGCTCTCATGCAAAATA+TGG | 0.294005 | 4.3:+53945053 | MS.gene45330:CDS |
| TCTTCCATATTAAGGAAATC+TGG | 0.329357 | 4.3:-53944919 | None:intergenic |
| TCTAAATTTGTTAGCAAGAC+TGG | 0.334083 | 4.3:+53944658 | MS.gene45330:CDS |
| TTTAACCAATCGTCAAAATC+AGG | 0.356658 | 4.3:-53945294 | None:intergenic |
| CTATTTGTTGATGAATTATG+AGG | 0.358221 | 4.3:-53945204 | None:intergenic |
| AAGTTGGATACTTCTTCTTA+AGG | 0.361888 | 4.3:-53944891 | None:intergenic |
| AGTTGGATACTTCTTCTTAA+GGG | 0.378758 | 4.3:-53944890 | None:intergenic |
| GGGTCTTCTGGCCATGTCAT+TGG | 0.398342 | 4.3:-53944730 | None:intergenic |
| TCTTAATTTCAAAGTATTTG+TGG | 0.402565 | 4.3:-53944825 | None:intergenic |
| TCAACAAATAGTGAGATGAT+TGG | 0.421059 | 4.3:+53945216 | MS.gene45330:CDS |
| TGATGTGTTGTTGCATTTGA+GGG | 0.426219 | 4.3:-53945137 | None:intergenic |
| AAGGGTATCTTTCCTCACTT+TGG | 0.441633 | 4.3:-53944872 | None:intergenic |
| AGCATGGAGTTTACAATTGT+AGG | 0.450184 | 4.3:-53944751 | None:intergenic |
| ATGAGGCAAATCAATATGAT+TGG | 0.457882 | 4.3:-53945187 | None:intergenic |
| TGCTATGCATGACATTAACT+TGG | 0.461959 | 4.3:-53945107 | None:intergenic |
| GCATGGAGTTTACAATTGTA+GGG | 0.478326 | 4.3:-53944750 | None:intergenic |
| TTGATGTGTTGTTGCATTTG+AGG | 0.480724 | 4.3:-53945138 | None:intergenic |
| GATTGGTTAAATCAACTAGA+TGG | 0.481740 | 4.3:+53945306 | MS.gene45330:CDS |
| AGGAAATCTGGATGCCAAGT+TGG | 0.499410 | 4.3:-53944907 | None:intergenic |
| AGTTGCTTTGTAGATGAACT+AGG | 0.501324 | 4.3:-53945264 | None:intergenic |
| TTGATTGTGTATGATGATGA+TGG | 0.536425 | 4.3:+53944694 | MS.gene45330:CDS |
| ATATGAGCATAAGAAGATTG+AGG | 0.538047 | 4.3:+53944780 | MS.gene45330:CDS |
| CAAATGCAACAACACATCAA+TGG | 0.552382 | 4.3:+53945141 | MS.gene45330:CDS |
| ATGCTCATATTCTTGAAGCA+TGG | 0.569236 | 4.3:-53944767 | None:intergenic |
| TGATGATGATGGTAATAATG+TGG | 0.569879 | 4.3:+53944705 | MS.gene45330:CDS |
| CATCAATGGTACTAATGAGA+TGG | 0.585095 | 4.3:+53945155 | MS.gene45330:CDS |
| ATTAACTTGGCTTGAATGTG+AGG | 0.596037 | 4.3:-53945094 | None:intergenic |
| CAATGGTACTAATGAGATGG+TGG | 0.610016 | 4.3:+53945158 | MS.gene45330:CDS |
| GATGATGATGGTAATAATGT+GGG | 0.631187 | 4.3:+53944706 | MS.gene45330:CDS |
| TTAAGAAGAAGTATCCAACT+TGG | 0.637255 | 4.3:+53944893 | MS.gene45330:CDS |
| ATAATGTGGGACCAATGACA+TGG | 0.658982 | 4.3:+53944719 | MS.gene45330:CDS |
| AAGCTAAGATAACCAAAGTG+AGG | 0.659175 | 4.3:+53944860 | MS.gene45330:CDS |
| TGGTTAAATCAACTAGATGG+TGG | 0.663107 | 4.3:+53945309 | MS.gene45330:CDS |
| TGCTTTGTAGATGAACTAGG+AGG | 0.674710 | 4.3:-53945261 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTAATTTCAAAGTATTTG+TGG | - | chr4.3:53944828-53944847 | None:intergenic | 20.0% |
| ! | GCTTTCAAAGAAAGAAAAAA+TGG | + | chr4.3:53944619-53944638 | MS.gene45330:CDS | 25.0% |
| !! | CTATTTGTTGATGAATTATG+AGG | - | chr4.3:53945207-53945226 | None:intergenic | 25.0% |
| !!! | AAAGAAAAAATGGATTAGCA+AGG | + | chr4.3:53944629-53944648 | MS.gene45330:CDS | 25.0% |
| !!! | GTTGATTTTCTTCCATATTA+AGG | - | chr4.3:53944930-53944949 | None:intergenic | 25.0% |
| AAGTTGGATACTTCTTCTTA+AGG | - | chr4.3:53944894-53944913 | None:intergenic | 30.0% | |
| AGTTGGATACTTCTTCTTAA+GGG | - | chr4.3:53944893-53944912 | None:intergenic | 30.0% | |
| ATATGAGCATAAGAAGATTG+AGG | + | chr4.3:53944780-53944799 | MS.gene45330:CDS | 30.0% | |
| ATGAGGCAAATCAATATGAT+TGG | - | chr4.3:53945190-53945209 | None:intergenic | 30.0% | |
| TCAACAAATAGTGAGATGAT+TGG | + | chr4.3:53945216-53945235 | MS.gene45330:CDS | 30.0% | |
| TCTTCCATATTAAGGAAATC+TGG | - | chr4.3:53944922-53944941 | None:intergenic | 30.0% | |
| TTGTGGACATCAAATATTTC+AGG | - | chr4.3:53944811-53944830 | None:intergenic | 30.0% | |
| TTTAACCAATCGTCAAAATC+AGG | - | chr4.3:53945297-53945316 | None:intergenic | 30.0% | |
| ! | GATTGGTTAAATCAACTAGA+TGG | + | chr4.3:53945306-53945325 | MS.gene45330:CDS | 30.0% |
| ! | TTGATTGTGTATGATGATGA+TGG | + | chr4.3:53944694-53944713 | MS.gene45330:CDS | 30.0% |
| !! | GATGATGATGGTAATAATGT+GGG | + | chr4.3:53944706-53944725 | MS.gene45330:CDS | 30.0% |
| !! | TCTAAATTTGTTAGCAAGAC+TGG | + | chr4.3:53944658-53944677 | MS.gene45330:CDS | 30.0% |
| !! | TGATGATGATGGTAATAATG+TGG | + | chr4.3:53944705-53944724 | MS.gene45330:CDS | 30.0% |
| !! | TTAAGAAGAAGTATCCAACT+TGG | + | chr4.3:53944893-53944912 | MS.gene45330:CDS | 30.0% |
| AAGCTAAGATAACCAAAGTG+AGG | + | chr4.3:53944860-53944879 | MS.gene45330:CDS | 35.0% | |
| AGCATGGAGTTTACAATTGT+AGG | - | chr4.3:53944754-53944773 | None:intergenic | 35.0% | |
| ATGCTCATATTCTTGAAGCA+TGG | - | chr4.3:53944770-53944789 | None:intergenic | 35.0% | |
| CAAATGCAACAACACATCAA+TGG | + | chr4.3:53945141-53945160 | MS.gene45330:CDS | 35.0% | |
| CAATGCTCTCATGCAAAATA+TGG | + | chr4.3:53945053-53945072 | MS.gene45330:CDS | 35.0% | |
| CATCAATGGTACTAATGAGA+TGG | + | chr4.3:53945155-53945174 | MS.gene45330:CDS | 35.0% | |
| GCATCCAGATTTCCTTAATA+TGG | + | chr4.3:53944915-53944934 | MS.gene45330:CDS | 35.0% | |
| GCATGGAGTTTACAATTGTA+GGG | - | chr4.3:53944753-53944772 | None:intergenic | 35.0% | |
| TGCTATGCATGACATTAACT+TGG | - | chr4.3:53945110-53945129 | None:intergenic | 35.0% | |
| TGGTTAAATCAACTAGATGG+TGG | + | chr4.3:53945309-53945328 | MS.gene45330:CDS | 35.0% | |
| TTTACAATTGTAGGGTCTTC+TGG | - | chr4.3:53944745-53944764 | None:intergenic | 35.0% | |
| ! | AGTTGCTTTGTAGATGAACT+AGG | - | chr4.3:53945267-53945286 | None:intergenic | 35.0% |
| ! | ATTAACTTGGCTTGAATGTG+AGG | - | chr4.3:53945097-53945116 | None:intergenic | 35.0% |
| ! | TGATGTGTTGTTGCATTTGA+GGG | - | chr4.3:53945140-53945159 | None:intergenic | 35.0% |
| ! | TTGATGTGTTGTTGCATTTG+AGG | - | chr4.3:53945141-53945160 | None:intergenic | 35.0% |
| ATAATGTGGGACCAATGACA+TGG | + | chr4.3:53944719-53944738 | MS.gene45330:CDS | 40.0% | |
| CAATGGTACTAATGAGATGG+TGG | + | chr4.3:53945158-53945177 | MS.gene45330:CDS | 40.0% | |
| TGCTTTGTAGATGAACTAGG+AGG | - | chr4.3:53945264-53945283 | None:intergenic | 40.0% | |
| ! | TGATTTTGTGATCACACGCT+TGG | - | chr4.3:53944987-53945006 | None:intergenic | 40.0% |
| !! | AAGGGTATCTTTCCTCACTT+TGG | - | chr4.3:53944875-53944894 | None:intergenic | 40.0% |
| !! | AGATGGTGGTGTTTTGAATC+AGG | + | chr4.3:53945323-53945342 | MS.gene45330:CDS | 40.0% |
| !! | GTCAACCTGATTTTGACGAT+TGG | + | chr4.3:53945289-53945308 | MS.gene45330:CDS | 40.0% |
| AGGAAATCTGGATGCCAAGT+TGG | - | chr4.3:53944910-53944929 | None:intergenic | 45.0% | |
| GGGTCTTCTGGCCATGTCAT+TGG | - | chr4.3:53944733-53944752 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 53944583 | 53945362 | 53944583 | ID=MS.gene45330 |
| chr4.3 | mRNA | 53944583 | 53945362 | 53944583 | ID=MS.gene45330.t1;Parent=MS.gene45330 |
| chr4.3 | exon | 53944583 | 53945362 | 53944583 | ID=MS.gene45330.t1.exon1;Parent=MS.gene45330.t1 |
| chr4.3 | CDS | 53944583 | 53945362 | 53944583 | ID=cds.MS.gene45330.t1;Parent=MS.gene45330.t1 |
| Gene Sequence |
| Protein sequence |
