Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45450.t1 | XP_027191036.1 | 88.7 | 53 | 6 | 0 | 9 | 61 | 131 | 183 | 8.30E-18 | 99 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45450.t1 | A0A3Q7Y126 | 88.7 | 53 | 6 | 0 | 9 | 61 | 131 | 183 | 6.0e-18 | 99.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene45450.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45450.t1 | MTR_1g056700 | 77.419 | 62 | 14 | 0 | 1 | 62 | 1 | 62 | 3.82e-28 | 96.3 |
| MS.gene45450.t1 | MTR_4g094762 | 86.275 | 51 | 7 | 0 | 11 | 61 | 272 | 322 | 8.33e-25 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTTACCTTAGTGGGATT+TGG | 0.241916 | 1.2:-44744048 | None:intergenic |
| GATTTACCTTAGTGGGATTT+GGG | 0.322473 | 1.2:-44744047 | None:intergenic |
| CTCCCATTCCTTGAGTTGGT+TGG | 0.408516 | 1.2:-44743894 | None:intergenic |
| AGCACTCCCATTCCTTGAGT+TGG | 0.421213 | 1.2:-44743898 | None:intergenic |
| AGCTAGAGATTTACCTTAGT+GGG | 0.447203 | 1.2:-44744054 | None:intergenic |
| AACTCAAGGAATGGGAGTGC+TGG | 0.459738 | 1.2:+44743900 | MS.gene45450:CDS |
| TGGGAGTGCTGGAATTCAGC+AGG | 0.485582 | 1.2:+44743911 | MS.gene45450:CDS |
| CAGCTAGAGATTTACCTTAG+TGG | 0.488189 | 1.2:-44744055 | None:intergenic |
| CAAAGGAGAGAGCAATTGAA+TGG | 0.490470 | 1.2:+44743997 | MS.gene45450:CDS |
| TTTCTCAGTTGCAAAATGTT+CGG | 0.492744 | 1.2:-44743974 | None:intergenic |
| TGCCAACCAACTCAAGGAAT+GGG | 0.494531 | 1.2:+44743892 | MS.gene45450:CDS |
| GCTTCTGTTGGCAAAGGAAG+AGG | 0.497466 | 1.2:+44743940 | MS.gene45450:CDS |
| AGCAGGGTCACTGCTTCTGT+TGG | 0.504668 | 1.2:+44743928 | MS.gene45450:CDS |
| ATGCCAACCAACTCAAGGAA+TGG | 0.516575 | 1.2:+44743891 | MS.gene45450:CDS |
| TGGAAACTACAAAATCTTGA+GGG | 0.544968 | 1.2:+44744017 | MS.gene45450:CDS |
| TTTCATCCCAAATCCCACTA+AGG | 0.563268 | 1.2:+44744041 | MS.gene45450:CDS |
| GTCACTGCTTCTGTTGGCAA+AGG | 0.564056 | 1.2:+44743934 | MS.gene45450:CDS |
| ATGGAAACTACAAAATCTTG+AGG | 0.571228 | 1.2:+44744016 | MS.gene45450:CDS |
| ATGGCATGCCAACCAACTCA+AGG | 0.607083 | 1.2:+44743886 | MS.gene45450:CDS |
| TCTGTTGGCAAAGGAAGAGG+AGG | 0.650064 | 1.2:+44743943 | MS.gene45450:CDS |
| GGGAGTGCTGGAATTCAGCA+GGG | 0.674436 | 1.2:+44743912 | MS.gene45450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGAAACTACAAAATCTTG+AGG | + | chr1.2:44744016-44744035 | MS.gene45450:CDS | 30.0% | |
| TGGAAACTACAAAATCTTGA+GGG | + | chr1.2:44744017-44744036 | MS.gene45450:CDS | 30.0% | |
| TTTCTCAGTTGCAAAATGTT+CGG | - | chr1.2:44743977-44743996 | None:intergenic | 30.0% | |
| ! | ATTTTGCAACTGAGAAACAA+AGG | + | chr1.2:44743980-44743999 | MS.gene45450:CDS | 30.0% |
| AGATTTACCTTAGTGGGATT+TGG | - | chr1.2:44744051-44744070 | None:intergenic | 35.0% | |
| GATTTACCTTAGTGGGATTT+GGG | - | chr1.2:44744050-44744069 | None:intergenic | 35.0% | |
| CAAAGGAGAGAGCAATTGAA+TGG | + | chr1.2:44743997-44744016 | MS.gene45450:CDS | 40.0% | |
| TTTCATCCCAAATCCCACTA+AGG | + | chr1.2:44744041-44744060 | MS.gene45450:CDS | 40.0% | |
| ATGCCAACCAACTCAAGGAA+TGG | + | chr1.2:44743891-44743910 | MS.gene45450:CDS | 45.0% | |
| TGCCAACCAACTCAAGGAAT+GGG | + | chr1.2:44743892-44743911 | MS.gene45450:CDS | 45.0% | |
| AGCACTCCCATTCCTTGAGT+TGG | - | chr1.2:44743901-44743920 | None:intergenic | 50.0% | |
| ATGGCATGCCAACCAACTCA+AGG | + | chr1.2:44743886-44743905 | MS.gene45450:CDS | 50.0% | |
| GCTTCTGTTGGCAAAGGAAG+AGG | + | chr1.2:44743940-44743959 | MS.gene45450:CDS | 50.0% | |
| GTCACTGCTTCTGTTGGCAA+AGG | + | chr1.2:44743934-44743953 | MS.gene45450:CDS | 50.0% | |
| TCTGTTGGCAAAGGAAGAGG+AGG | + | chr1.2:44743943-44743962 | MS.gene45450:CDS | 50.0% | |
| ! | AACTCAAGGAATGGGAGTGC+TGG | + | chr1.2:44743900-44743919 | MS.gene45450:CDS | 50.0% |
| !! | CTCCCATTCCTTGAGTTGGT+TGG | - | chr1.2:44743897-44743916 | None:intergenic | 50.0% |
| AGCAGGGTCACTGCTTCTGT+TGG | + | chr1.2:44743928-44743947 | MS.gene45450:CDS | 55.0% | |
| !! | GGGAGTGCTGGAATTCAGCA+GGG | + | chr1.2:44743912-44743931 | MS.gene45450:CDS | 55.0% |
| !! | TGGGAGTGCTGGAATTCAGC+AGG | + | chr1.2:44743911-44743930 | MS.gene45450:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 44743886 | 44744074 | 44743886 | ID=MS.gene45450 |
| chr1.2 | mRNA | 44743886 | 44744074 | 44743886 | ID=MS.gene45450.t1;Parent=MS.gene45450 |
| chr1.2 | exon | 44743886 | 44744074 | 44743886 | ID=MS.gene45450.t1.exon1;Parent=MS.gene45450.t1 |
| chr1.2 | CDS | 44743886 | 44744074 | 44743886 | ID=cds.MS.gene45450.t1;Parent=MS.gene45450.t1 |
| Gene Sequence |
| Protein sequence |
