AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46331

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46331.t1 MTR_4g097950 91.266 229 17 2 2 229 26 252 4.30e-135 380
MS.gene46331.t1 MTR_5g014600 53.571 224 94 4 6 225 116 333 8.05e-66 207
MS.gene46331.t1 MTR_4g092700 54.749 179 70 4 45 222 158 326 5.53e-51 169
MS.gene46331.t1 MTR_2g010450 49.730 185 83 4 45 228 177 352 2.30e-50 167
MS.gene46331.t1 MTR_5g014520 47.788 226 102 5 2 224 115 327 3.08e-50 167
MS.gene46331.t1 MTR_0250s0040 44.560 193 96 3 37 228 80 262 4.69e-47 156
MS.gene46331.t1 MTR_0246s0020 46.009 213 105 5 14 224 168 372 4.54e-45 154
MS.gene46331.t1 MTR_4g009540 42.991 214 111 4 15 222 118 326 1.25e-43 149
MS.gene46331.t1 MTR_5g014560 43.655 197 102 3 29 225 115 302 5.47e-43 147
MS.gene46331.t1 MTR_6g047570 44.681 188 93 3 37 223 151 328 6.66e-43 148
MS.gene46331.t1 MTR_4g009540 44.444 198 99 4 15 206 118 310 5.05e-41 142
MS.gene46331.t1 MTR_0246s0020 46.231 199 97 5 14 210 168 358 5.85e-41 143
MS.gene46331.t1 MTR_4g097940 44.149 188 86 4 45 228 155 327 1.09e-40 142
MS.gene46331.t1 MTR_0011s0210 45.810 179 86 3 45 222 183 351 1.25e-40 142
MS.gene46331.t1 MTR_4g067010 42.932 191 89 3 42 228 144 318 1.73e-40 141
MS.gene46331.t1 MTR_0011s0260 46.369 179 85 3 45 222 183 351 2.10e-40 142
MS.gene46331.t1 MTR_4g066460 43.590 195 98 2 34 228 144 326 7.25e-40 140
MS.gene46331.t1 MTR_4g009110 43.575 179 93 3 45 222 174 345 6.78e-39 137
MS.gene46331.t1 MTR_2g104550 41.935 217 111 6 8 222 104 307 1.59e-38 136
MS.gene46331.t1 MTR_4g097920 41.622 185 95 3 45 228 140 312 1.64e-36 130
MS.gene46331.t1 MTR_4g097920 41.622 185 95 3 45 228 155 327 2.76e-36 130
MS.gene46331.t1 MTR_0246s0050 40.099 202 102 3 24 224 131 314 4.79e-36 129
MS.gene46331.t1 MTR_4g098035 40.099 202 102 3 24 224 152 335 7.89e-36 129
MS.gene46331.t1 MTR_8g009020 42.473 186 99 3 45 227 143 323 7.55e-35 127
MS.gene46331.t1 MTR_2g104490 43.229 192 95 6 31 221 124 302 2.14e-34 125
MS.gene46331.t1 MTR_4g066380 41.969 193 99 2 36 228 144 323 1.04e-33 124
MS.gene46331.t1 MTR_2g104490 44.633 177 84 6 31 206 124 287 1.28e-32 120
MS.gene46331.t1 MTR_2g104560 38.830 188 94 4 46 228 55 226 2.76e-32 117
MS.gene46331.t1 MTR_5g014640 36.869 198 113 1 28 225 137 322 7.71e-31 116
MS.gene46331.t1 MTR_2g104500 34.450 209 122 2 14 222 74 267 2.10e-30 114
MS.gene46331.t1 MTR_7g080780 37.705 183 96 2 45 227 152 316 3.22e-28 108
MS.gene46331.t1 MTR_2g104530 36.517 178 98 2 46 222 106 269 3.85e-28 108
MS.gene46331.t1 MTR_7g080780 37.705 183 96 2 45 227 165 329 4.03e-28 108
MS.gene46331.t1 MTR_2g104590 37.569 181 86 5 43 223 67 220 1.45e-26 102
MS.gene46331.t1 MTR_2g104650 37.500 200 112 4 25 223 78 265 1.44e-25 101
MS.gene46331.t1 MTR_8g009010 37.430 179 66 2 45 222 134 267 8.32e-25 100
MS.gene46331.t1 MTR_8g069740 37.725 167 95 3 43 208 110 268 1.49e-24 98.2
MS.gene46331.t1 MTR_5g014550 45.038 131 63 2 75 205 54 175 4.63e-24 95.5
MS.gene46331.t1 MTR_6g047550 56.322 87 38 0 26 112 154 240 1.08e-23 96.3
MS.gene46331.t1 MTR_8g064700 31.313 198 126 3 25 222 224 411 2.23e-22 94.7
MS.gene46331.t1 MTR_7g451650 33.974 156 84 4 71 226 60 196 7.16e-19 81.6
MS.gene46331.t1 MTR_4g009420 30.409 171 91 3 56 226 54 196 2.71e-18 80.1
MS.gene46331.t1 MTR_4g009510 32.597 181 100 4 44 223 42 201 3.31e-18 80.1
MS.gene46331.t1 MTR_7g050350 32.680 153 84 4 74 226 1 134 6.58e-18 77.4
MS.gene46331.t1 MTR_7g451650 32.680 153 84 4 74 226 1 134 6.58e-18 77.4
MS.gene46331.t1 MTR_8g067280 35.762 151 87 3 45 189 462 608 4.93e-16 77.0
MS.gene46331.t1 MTR_8g024790 30.159 189 109 6 30 211 276 448 8.86e-16 75.9
MS.gene46331.t1 MTR_7g117670 54.237 59 27 0 45 103 402 460 1.29e-15 75.5
MS.gene46331.t1 MTR_8g027495 55.357 56 25 0 45 100 458 513 6.33e-15 73.6
MS.gene46331.t1 MTR_7g096350 55.556 54 24 0 45 98 355 408 1.29e-14 72.8
MS.gene46331.t1 MTR_5g030430 42.045 88 42 1 11 98 468 546 1.70e-14 72.4
MS.gene46331.t1 MTR_8g024770 30.435 161 102 3 2 162 241 391 5.43e-14 70.9
MS.gene46331.t1 MTR_7g451650 35.294 136 69 4 71 206 60 176 1.40e-13 67.4
MS.gene46331.t1 MTR_1g072320 46.032 63 34 0 45 107 470 532 1.42e-13 69.7
MS.gene46331.t1 MTR_2g084280 29.348 184 115 4 47 229 135 304 2.11e-13 68.6
MS.gene46331.t1 MTR_4g009420 31.655 139 68 2 68 206 65 176 5.94e-13 65.9
MS.gene46331.t1 MTR_3g030700 30.247 162 103 3 47 205 198 352 6.88e-13 67.4
MS.gene46331.t1 MTR_3g498695 27.273 198 116 6 13 200 275 454 1.49e-12 66.6
MS.gene46331.t1 MTR_3g498695 27.273 198 116 6 13 200 275 454 1.56e-12 66.6
MS.gene46331.t1 MTR_2g081040 30.435 161 99 4 47 200 179 333 2.55e-12 65.5
MS.gene46331.t1 MTR_7g083900 27.673 159 108 3 47 200 288 444 5.56e-12 65.1
MS.gene46331.t1 MTR_7g083900 27.673 159 108 3 47 200 288 444 5.61e-12 64.7
MS.gene46331.t1 MTR_3g104100 27.950 161 103 3 47 200 197 351 7.13e-12 64.3
MS.gene46331.t1 MTR_2g038040 26.943 193 123 6 46 229 53 236 1.94e-11 62.0
MS.gene46331.t1 MTR_1g080890 34.951 103 60 1 43 145 201 296 2.82e-11 62.4
MS.gene46331.t1 MTR_4g057270 26.250 160 102 4 47 202 151 298 5.43e-11 61.6
MS.gene46331.t1 MTR_1g112680 44.068 59 33 0 47 105 319 377 5.50e-11 62.0
MS.gene46331.t1 MTR_4g131160 34.228 149 79 5 43 186 207 341 7.32e-11 61.6
MS.gene46331.t1 MTR_4g131160 34.228 149 79 5 43 186 204 338 7.63e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46331.t1 AT4G37850 49.457 184 85 3 45 228 152 327 1.08e-49 165
MS.gene46331.t1 AT4G37850 49.457 184 85 3 45 228 152 327 1.08e-49 165
MS.gene46331.t1 AT2G22750 49.246 199 95 4 30 228 112 304 1.61e-42 146
MS.gene46331.t1 AT2G22750 49.246 199 95 4 30 228 136 328 2.56e-42 146
MS.gene46331.t1 AT2G22750 49.246 199 95 4 30 228 140 332 2.69e-42 146
MS.gene46331.t1 AT2G22750 49.246 199 94 5 30 228 112 303 4.84e-41 142
MS.gene46331.t1 AT2G22750 49.246 199 94 5 30 228 140 331 5.94e-41 142
MS.gene46331.t1 AT2G22760 40.796 201 104 5 33 225 101 294 4.56e-39 137
MS.gene46331.t1 AT2G22770 38.342 193 113 2 41 227 128 320 3.12e-36 130
MS.gene46331.t1 AT2G22760 40.884 181 93 4 33 206 101 274 3.69e-34 124
MS.gene46331.t1 AT2G46510 58.929 56 23 0 45 100 395 450 2.50e-17 80.9
MS.gene46331.t1 AT4G29930 28.311 219 130 7 27 229 39 246 1.25e-16 76.6
MS.gene46331.t1 AT5G46760 34.437 151 82 4 45 189 415 554 1.56e-16 78.6
MS.gene46331.t1 AT4G29930 30.693 202 104 8 27 206 39 226 1.36e-15 73.9
MS.gene46331.t1 AT4G29930 30.693 202 104 8 27 206 39 226 1.36e-15 73.9
MS.gene46331.t1 AT1G01260 29.310 174 77 4 18 188 415 545 1.52e-15 75.5
MS.gene46331.t1 AT1G01260 29.310 174 77 4 18 188 415 545 1.52e-15 75.5
MS.gene46331.t1 AT1G01260 29.310 174 77 4 18 188 415 545 1.52e-15 75.5
MS.gene46331.t1 AT2G16910 28.249 177 114 3 47 210 316 492 3.75e-15 74.3
MS.gene46331.t1 AT1G32640 62.000 50 19 0 45 94 452 501 1.79e-14 72.4
MS.gene46331.t1 AT4G29930 26.699 206 124 6 27 229 39 220 1.86e-14 70.5
MS.gene46331.t1 AT4G29930 28.502 207 116 8 27 229 39 217 2.03e-14 70.1
MS.gene46331.t1 AT4G17880 32.468 154 77 3 45 189 416 551 5.77e-14 70.9
MS.gene46331.t1 AT5G58010 40.741 108 57 2 43 146 107 211 9.25e-14 69.3
MS.gene46331.t1 AT4G29930 29.891 184 100 7 27 206 39 197 1.87e-13 67.8
MS.gene46331.t1 AT4G16430 35.165 91 50 1 8 98 292 373 2.17e-13 68.9
MS.gene46331.t1 AT1G12860 29.167 168 106 3 47 209 269 428 9.64e-13 67.0
MS.gene46331.t1 AT1G12860 30.189 159 98 3 47 200 269 419 1.21e-12 67.0
MS.gene46331.t1 AT5G57150 26.214 206 137 4 34 229 34 234 1.62e-12 65.5
MS.gene46331.t1 AT4G09820 27.536 138 71 2 46 183 364 472 1.64e-12 66.6
MS.gene46331.t1 AT5G57150 29.240 171 108 4 34 192 35 204 2.88e-12 64.3
MS.gene46331.t1 AT5G57150 29.240 171 108 4 34 192 35 204 3.13e-12 64.3
MS.gene46331.t1 AT5G57150 29.240 171 108 4 34 192 34 203 3.48e-12 63.9
MS.gene46331.t1 AT5G57150 28.655 171 109 4 34 192 35 204 4.37e-12 64.3
MS.gene46331.t1 AT5G46830 50.000 54 27 0 45 98 343 396 1.60e-11 63.5
MS.gene46331.t1 AT2G24260 43.299 97 44 2 43 131 43 136 1.82e-11 62.4
MS.gene46331.t1 AT4G29930 34.737 95 60 1 47 141 56 148 2.01e-11 61.2
MS.gene46331.t1 AT2G28160 28.571 189 96 6 47 217 133 300 2.60e-11 62.4
MS.gene46331.t1 AT2G28160 28.571 189 96 6 47 217 133 300 3.17e-11 62.4
MS.gene46331.t1 AT4G00870 33.000 100 65 1 5 102 207 306 3.62e-11 62.4
MS.gene46331.t1 AT1G63650 27.322 183 122 5 29 202 390 570 4.47e-11 62.4
MS.gene46331.t1 AT1G63650 27.322 183 122 5 29 202 390 570 4.47e-11 62.4
MS.gene46331.t1 AT1G63650 27.322 183 122 5 29 202 390 570 4.47e-11 62.4
MS.gene46331.t1 AT2G24260 43.299 97 44 2 43 131 146 239 6.26e-11 61.6
MS.gene46331.t1 AT5G41315 27.586 174 111 3 43 202 444 616 9.41e-11 61.6
MS.gene46331.t1 AT5G41315 27.586 174 111 3 43 202 439 611 9.84e-11 61.2

Find 44 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAATTGCTGAGAGAATA+AGG 0.332685 8.4:+24603891 MS.gene46331:CDS
CCTTATTCCAAACCTCAAAA+AGG 0.354140 8.4:+24603952 MS.gene46331:CDS
TTCATTTGGTTCCTCTGCTC+TGG 0.370847 8.4:+24604809 MS.gene46331:CDS
CGGGGAAGAATGTGCTGATT+AGG 0.377824 8.4:+24604697 MS.gene46331:CDS
GAACATGAGCATCCAGAAAC+TGG 0.387862 8.4:+24605728 MS.gene46331:CDS
GAAGTTAAAGCTAGTTTGTC+GGG 0.404928 8.4:+24604678 MS.gene46331:CDS
AATTTCTTCACAACCGTAAC+CGG 0.405974 8.4:-24604558 None:intergenic
AATAGAGAAACTTGGTCTCT+TGG 0.416449 8.4:+24604767 MS.gene46331:CDS
AAGGTTGGTACAACCTTCAT+TGG 0.429134 8.4:-24603752 None:intergenic
CTCTCAGCAATTATATGATC+TGG 0.438168 8.4:-24603884 None:intergenic
ATTCTTTGAGTTCACAACTT+TGG 0.438205 8.4:-24603786 None:intergenic
GACAAACTAGCTTTAACTTC+AGG 0.442233 8.4:-24604675 None:intergenic
GTCTGCGGATGTCAATGTTT+CGG 0.442533 8.4:+24604590 MS.gene46331:CDS
ATGGACAAGGCCTCGGTGTT+GGG 0.445192 8.4:+24604456 MS.gene46331:CDS
CAAAAGCAAAAGTGTAGAAC+CGG 0.446629 8.4:+24604539 MS.gene46331:CDS
GTTTCCATTACATGAATTTG+AGG 0.464190 8.4:-24604619 None:intergenic
AAAGTATGTGAAGGAGCTTA+AGG 0.470002 8.4:+24604488 MS.gene46331:CDS
CAAAAGTGTAGAACCGGTTA+CGG 0.471591 8.4:+24604545 MS.gene46331:CDS
ACTTCCTCAAATTCATGTAA+TGG 0.472899 8.4:+24604615 MS.gene46331:CDS
TGTGTGATGAGTTGTAGATT+TGG 0.488926 8.4:-24603861 None:intergenic
AAAATGTACCTGAGCAATGA+TGG 0.489397 8.4:-24604838 None:intergenic
GATGGACAAGGCCTCGGTGT+TGG 0.491255 8.4:+24604455 MS.gene46331:intron
AAAGTTGTGAACTCAAAGAA+TGG 0.494937 8.4:+24603788 MS.gene46331:CDS
TTTGTAGATGGACAAGGCCT+CGG 0.501079 8.4:+24604449 MS.gene46331:intron
GGCCATAACCATCATTGCTC+AGG 0.505461 8.4:+24604830 MS.gene46331:CDS
TGAGAAAGATAAGGCAGTGA+TGG 0.525707 8.4:+24604728 MS.gene46331:CDS
TAAGGAACAAGTAAAACTGT+TGG 0.530262 8.4:+24604506 MS.gene46331:CDS
CTGAAAGAGCTATGAACTGT+TGG 0.532934 8.4:-24603928 None:intergenic
TGAAGTTAAAGCTAGTTTGT+CGG 0.533689 8.4:+24604677 MS.gene46331:CDS
GAGAAAGATAAGGCAGTGAT+GGG 0.538793 8.4:+24604729 MS.gene46331:CDS
GGAACAAGTAAAACTGTTGG+AGG 0.550452 8.4:+24604509 MS.gene46331:CDS
TATAGAGAAATAGAGAAACT+TGG 0.574558 8.4:+24604759 MS.gene46331:CDS
TACCTGAGCAATGATGGTTA+TGG 0.585304 8.4:-24604832 None:intergenic
GATCCTCTGTGAGAAAGATA+AGG 0.589750 8.4:+24604719 MS.gene46331:CDS
GATGGTTATGGCCAGAGCAG+AGG 0.614054 8.4:-24604820 None:intergenic
CAGGAGAGCAACAAGAAGAA+CGG 0.634139 8.4:+24603830 MS.gene46331:CDS
AAGTTAAAGCTAGTTTGTCG+GGG 0.643559 8.4:+24604679 MS.gene46331:CDS
GAAATTGTCTGAACTGTCTG+CGG 0.654800 8.4:+24604575 MS.gene46331:CDS
TGAAGGTTGTACCAACCTTG+AGG 0.663007 8.4:+24603756 MS.gene46331:CDS
CATATGTTTGTAGATGGACA+AGG 0.664887 8.4:+24604443 MS.gene46331:intron
AGAAAGATAAGGCAGTGATG+GGG 0.666003 8.4:+24604730 MS.gene46331:CDS
CTGCCTTATCTTTCTCACAG+AGG 0.691029 8.4:-24604722 None:intergenic
TGATGCTATAAAGTATGTGA+AGG 0.702262 8.4:+24604479 MS.gene46331:CDS
TATAGCATCACCCAACACCG+AGG 0.755825 8.4:-24604466 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATTTCATATTAAAAAA+TGG + chr8.4:24605504-24605523 MS.gene46331:intron 10.0%
!! AATAAAAATTGCATTAAGAT+CGG - chr8.4:24605074-24605093 None:intergenic 15.0%
!! ATTGTTAAAAAAATGGTATA+AGG - chr8.4:24605332-24605351 None:intergenic 15.0%
!!! ATTTTTAATTAAGGACTATA+TGG + chr8.4:24604856-24604875 MS.gene46331:intron 15.0%
!! AAGTTCAATGTAATATTACT+CGG + chr8.4:24605247-24605266 MS.gene46331:intron 20.0%
!! ACAACACATTGTTAAAAAAA+TGG - chr8.4:24605339-24605358 None:intergenic 20.0%
!! CATCTAAATAATTGTATACA+TGG + chr8.4:24604012-24604031 MS.gene46331:intron 20.0%
!! TAACGTAAAAGTCATATAAA+TGG + chr8.4:24604208-24604227 MS.gene46331:intron 20.0%
!!! ATATAAATGGTATTTCTGAT+TGG + chr8.4:24604221-24604240 MS.gene46331:intron 20.0%
!!! ATTTTTTTAACAATGTGTTG+TGG + chr8.4:24605338-24605357 MS.gene46331:intron 20.0%
!!! TTTTTAGATTGTATGTCAAT+CGG + chr8.4:24604143-24604162 MS.gene46331:intron 20.0%
! ATATAATTGCTGAGAGAATA+AGG + chr8.4:24603891-24603910 MS.gene46331:CDS 25.0%
! TATAGAGAAATAGAGAAACT+TGG + chr8.4:24604759-24604778 MS.gene46331:CDS 25.0%
!! AGCATTGTTTAATTTCGATA+CGG - chr8.4:24604411-24604430 None:intergenic 25.0%
!! TTGAAAAATGAACCTTTTTG+AGG - chr8.4:24603967-24603986 None:intergenic 25.0%
!!! CTCAGGTACATTTTTAATTA+AGG + chr8.4:24604847-24604866 MS.gene46331:intron 25.0%
!!! TGAAAAAGTTTTAGTTTGCA+AGG + chr8.4:24605415-24605434 MS.gene46331:intron 25.0%
!!! TTTTTATTGATGTGTTGACT+AGG + chr8.4:24605086-24605105 MS.gene46331:intron 25.0%
AAAGTTGTGAACTCAAAGAA+TGG + chr8.4:24603788-24603807 MS.gene46331:CDS 30.0%
AAATACGCAAACATCATGAA+TGG - chr8.4:24604973-24604992 None:intergenic 30.0%
AATGTAGTGTCAAATTGACT+TGG + chr8.4:24605217-24605236 MS.gene46331:intron 30.0%
AATTGTGTCTTTATGGTGTT+TGG + chr8.4:24605688-24605707 MS.gene46331:intron 30.0%
ACACTCTTAAGACATTATGT+TGG - chr8.4:24605187-24605206 None:intergenic 30.0%
ACTTCCTCAAATTCATGTAA+TGG + chr8.4:24604615-24604634 MS.gene46331:CDS 30.0%
AGAGTAATATCTCATCACTA+TGG + chr8.4:24605647-24605666 MS.gene46331:intron 30.0%
ATACAAAAAAACGTGAGAGA+TGG + chr8.4:24605110-24605129 MS.gene46331:intron 30.0%
ATGGAAATCAATTTCTTAGC+GGG + chr8.4:24605129-24605148 MS.gene46331:intron 30.0%
ATTGACCATGATACCATTAT+TGG - chr8.4:24605296-24605315 None:intergenic 30.0%
CATATACTTAGATGAGTGAA+TGG + chr8.4:24605442-24605461 MS.gene46331:intron 30.0%
GATCGATGTATTAATGCTTT+GGG + chr8.4:24605562-24605581 MS.gene46331:intron 30.0%
GATGGAAATCAATTTCTTAG+CGG + chr8.4:24605128-24605147 MS.gene46331:intron 30.0%
GTTTCCATTACATGAATTTG+AGG - chr8.4:24604622-24604641 None:intergenic 30.0%
TAAGGAACAAGTAAAACTGT+TGG + chr8.4:24604506-24604525 MS.gene46331:CDS 30.0%
TCTCGTTGATGTTGTATTAA+CGG + chr8.4:24605373-24605392 MS.gene46331:intron 30.0%
TGATGCTATAAAGTATGTGA+AGG + chr8.4:24604479-24604498 MS.gene46331:CDS 30.0%
TGTCATAAAATGTGTTTAGC+AGG + chr8.4:24604913-24604932 MS.gene46331:intron 30.0%
TTGAACATCACAAAAGTTAG+AGG + chr8.4:24605529-24605548 MS.gene46331:intron 30.0%
TTGACGTAATTGTGTCTTTA+TGG + chr8.4:24605681-24605700 MS.gene46331:intron 30.0%
! AATGCTAGTTCGTTTTCATT+TGG + chr8.4:24604795-24604814 MS.gene46331:CDS 30.0%
! ATTCTTTGAGTTCACAACTT+TGG - chr8.4:24603789-24603808 None:intergenic 30.0%
! TGAAGTTAAAGCTAGTTTGT+CGG + chr8.4:24604677-24604696 MS.gene46331:CDS 30.0%
! TGTATTAATGCTTTGGGTAA+TGG + chr8.4:24605568-24605587 MS.gene46331:intron 30.0%
! TTTTCGTAAGGATCCAATAA+TGG + chr8.4:24605280-24605299 MS.gene46331:intron 30.0%
!!! AAATGAACCTTTTTGAGGTT+TGG - chr8.4:24603962-24603981 None:intergenic 30.0%
!!! CTGTTTTTAATTAGCATGCA+AGG - chr8.4:24605160-24605179 None:intergenic 30.0%
!!! CTTTTTGAGGTTTGGAATAA+GGG - chr8.4:24603954-24603973 None:intergenic 30.0%
!!! GGTGTTTTTAGTTTTTCGTA+AGG + chr8.4:24605268-24605287 MS.gene46331:intron 30.0%
AAAAAAACTGAGAGTTGGAC+TGG + chr8.4:24605752-24605771 MS.gene46331:CDS 35.0%
AAAATGTACCTGAGCAATGA+TGG - chr8.4:24604841-24604860 None:intergenic 35.0%
AAAATGTGTTTAGCAGGAGA+GGG + chr8.4:24604919-24604938 MS.gene46331:intron 35.0%
AAAGTATGTGAAGGAGCTTA+AGG + chr8.4:24604488-24604507 MS.gene46331:CDS 35.0%
AACAATGTGTTGTGGTCAAT+TGG + chr8.4:24605346-24605365 MS.gene46331:intron 35.0%
AATAGAGAAACTTGGTCTCT+TGG + chr8.4:24604767-24604786 MS.gene46331:CDS 35.0%
AATTTCTTCACAACCGTAAC+CGG - chr8.4:24604561-24604580 None:intergenic 35.0%
ACCAAGACGTGTAAAAAATC+AGG + chr8.4:24603811-24603830 MS.gene46331:CDS 35.0%
AGGATCCAATAATGGTATCA+TGG + chr8.4:24605288-24605307 MS.gene46331:intron 35.0%
CATATGTTTGTAGATGGACA+AGG + chr8.4:24604443-24604462 MS.gene46331:intron 35.0%
CCTTATTCCAAACCTCAAAA+AGG + chr8.4:24603952-24603971 MS.gene46331:CDS 35.0%
CGATCGATGTATTAATGCTT+TGG + chr8.4:24605561-24605580 MS.gene46331:intron 35.0%
CTCTCAGCAATTATATGATC+TGG - chr8.4:24603887-24603906 None:intergenic 35.0%
CTTGTCCATCTACAAACATA+TGG - chr8.4:24604445-24604464 None:intergenic 35.0%
GTACTCCATATGTTTGTAGA+TGG + chr8.4:24604437-24604456 MS.gene46331:intron 35.0%
TAAAATGTGTTTAGCAGGAG+AGG + chr8.4:24604918-24604937 MS.gene46331:intron 35.0%
TCATGATGTTTGCGTATTTC+TGG + chr8.4:24604974-24604993 MS.gene46331:intron 35.0%
TGTGTGATGAGTTGTAGATT+TGG - chr8.4:24603864-24603883 None:intergenic 35.0%
! AAGTTAAAGCTAGTTTGTCG+GGG + chr8.4:24604679-24604698 MS.gene46331:CDS 35.0%
! AATGCTTTGGGTAATGGTAA+TGG + chr8.4:24605574-24605593 MS.gene46331:intron 35.0%
! CAAAAGCAAAAGTGTAGAAC+CGG + chr8.4:24604539-24604558 MS.gene46331:CDS 35.0%
! GAAGTTAAAGCTAGTTTGTC+GGG + chr8.4:24604678-24604697 MS.gene46331:CDS 35.0%
!! GACAAACTAGCTTTAACTTC+AGG - chr8.4:24604678-24604697 None:intergenic 35.0%
!!! AACTTTGGTTTTTGACCTCA+AGG - chr8.4:24603774-24603793 None:intergenic 35.0%
!!! CCTTTTTGAGGTTTGGAATA+AGG - chr8.4:24603955-24603974 None:intergenic 35.0%
!!! TCAGTTTTTTTGCCAGTTTC+TGG - chr8.4:24605743-24605762 None:intergenic 35.0%
!!! TCCTGATTTTTTACACGTCT+TGG - chr8.4:24603815-24603834 None:intergenic 35.0%
AATGGATGCAATCCAACAGA+AGG - chr8.4:24604955-24604974 None:intergenic 40.0%
AGAAAGATAAGGCAGTGATG+GGG + chr8.4:24604730-24604749 MS.gene46331:CDS 40.0%
ATGGATGCAATCCAACAGAA+GGG - chr8.4:24604954-24604973 None:intergenic 40.0%
CTGAAAGAGCTATGAACTGT+TGG - chr8.4:24603931-24603950 None:intergenic 40.0%
CTGGCAAAAAAACTGAGAGT+TGG + chr8.4:24605747-24605766 MS.gene46331:CDS 40.0%
GAAATCAATTTCTTAGCGGG+AGG + chr8.4:24605132-24605151 MS.gene46331:intron 40.0%
GAAATTGTCTGAACTGTCTG+CGG + chr8.4:24604575-24604594 MS.gene46331:CDS 40.0%
GAGAAAGATAAGGCAGTGAT+GGG + chr8.4:24604729-24604748 MS.gene46331:CDS 40.0%
GATCCTCTGTGAGAAAGATA+AGG + chr8.4:24604719-24604738 MS.gene46331:CDS 40.0%
GGAACAAGTAAAACTGTTGG+AGG + chr8.4:24604509-24604528 MS.gene46331:CDS 40.0%
TACCTGAGCAATGATGGTTA+TGG - chr8.4:24604835-24604854 None:intergenic 40.0%
TCTTTATGGTGTTTGGCAGA+TGG + chr8.4:24605695-24605714 MS.gene46331:intron 40.0%
TGAGAAAGATAAGGCAGTGA+TGG + chr8.4:24604728-24604747 MS.gene46331:CDS 40.0%
! CAAAAGTGTAGAACCGGTTA+CGG + chr8.4:24604545-24604564 MS.gene46331:CDS 40.0%
! TCTGATTGGTTGACACAGTA+AGG + chr8.4:24604235-24604254 MS.gene46331:intron 40.0%
!! TTGGTTTTTGACCTCAAGGT+TGG - chr8.4:24603770-24603789 None:intergenic 40.0%
CAGGAGAGCAACAAGAAGAA+CGG + chr8.4:24603830-24603849 MS.gene46331:CDS 45.0%
CTGCCTTATCTTTCTCACAG+AGG - chr8.4:24604725-24604744 None:intergenic 45.0%
GAACATGAGCATCCAGAAAC+TGG + chr8.4:24605728-24605747 MS.gene46331:CDS 45.0%
GGATATGGTTTCCCTTCTGT+TGG + chr8.4:24604940-24604959 MS.gene46331:intron 45.0%
GTCTGCGGATGTCAATGTTT+CGG + chr8.4:24604590-24604609 MS.gene46331:CDS 45.0%
TGAAGGTTGTACCAACCTTG+AGG + chr8.4:24603756-24603775 MS.gene46331:CDS 45.0%
TGTTTAGCAGGAGAGGGATA+TGG + chr8.4:24604925-24604944 MS.gene46331:intron 45.0%
TTTGTAGATGGACAAGGCCT+CGG + chr8.4:24604449-24604468 MS.gene46331:intron 45.0%
! GTGAATGGACTCACACTTGA+AGG + chr8.4:24605457-24605476 MS.gene46331:intron 45.0%
! TTCATTTGGTTCCTCTGCTC+TGG + chr8.4:24604809-24604828 MS.gene46331:CDS 45.0%
!! GCTTTGGGTAATGGTAATGG+TGG + chr8.4:24605577-24605596 MS.gene46331:intron 45.0%
!! GGGTAATGGTAATGGTGGTA+AGG + chr8.4:24605582-24605601 MS.gene46331:intron 45.0%
!!! AAATTTTAAACTTAATATTT+TGG + chr8.4:24605022-24605041 MS.gene46331:intron 5.0%
GGCCATAACCATCATTGCTC+AGG + chr8.4:24604830-24604849 MS.gene46331:CDS 50.0%
TATAGCATCACCCAACACCG+AGG - chr8.4:24604469-24604488 None:intergenic 50.0%
!! CGGGGAAGAATGTGCTGATT+AGG + chr8.4:24604697-24604716 MS.gene46331:CDS 50.0%
GATGGTTATGGCCAGAGCAG+AGG - chr8.4:24604823-24604842 None:intergenic 55.0%
!! ATGGACAAGGCCTCGGTGTT+GGG + chr8.4:24604456-24604475 MS.gene46331:CDS 55.0%
! GATGGACAAGGCCTCGGTGT+TGG + chr8.4:24604455-24604474 MS.gene46331:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 24603755 24605788 24603755 ID=MS.gene46331
chr8.4 mRNA 24603755 24605788 24603755 ID=MS.gene46331.t1;Parent=MS.gene46331
chr8.4 exon 24603755 24603973 24603755 ID=MS.gene46331.t1.exon1;Parent=MS.gene46331.t1
chr8.4 CDS 24603755 24603973 24603755 ID=cds.MS.gene46331.t1;Parent=MS.gene46331.t1
chr8.4 exon 24604456 24604851 24604456 ID=MS.gene46331.t1.exon2;Parent=MS.gene46331.t1
chr8.4 CDS 24604456 24604851 24604456 ID=cds.MS.gene46331.t1;Parent=MS.gene46331.t1
chr8.4 exon 24605714 24605788 24605714 ID=MS.gene46331.t1.exon3;Parent=MS.gene46331.t1
chr8.4 CDS 24605714 24605788 24605714 ID=cds.MS.gene46331.t1;Parent=MS.gene46331.t1
Gene Sequence

>MS.gene46331

ATGAAGGTTGTACCAACCTTGAGGTCAAAAACCAAAGTTGTGAACTCAAAGAATGGACCAAGACGTGTAAAAAATCAGGAGAGCAACAAGAAGAACGGTTCACTTTCCAAATCTACAACTCATCACACACCAGATCATATAATTGCTGAGAGAATAAGGAGAGAAAAGATTAGCCAACAGTTCATAGCTCTTTCAGCCCTTATTCCAAACCTCAAAAAGGTTCATTTTTCAATTTATACACATTAATTTGACTTAAACATCTAAATAATTGTATACATGGTACTATATTATTCTTGTGCGTATACTAATATGATTCAATTCATATACAATTTGATTACTAAGTTTCTTTTTTTGAGATTTAATTTATATTCATTCATAAAGTAAATATTTTTTAGATTGTATGTCAATCGGATCAATCATAATCGTTCGATAATTAAAAAAGTTTAACTTTCATAACGTAAAAGTCATATAAATGGTATTTCTGATTGGTTGACACAGTAAGGAGTTCAAAACTATTAGTTCATCAGCTTTTGTTATATTGTCAATCAATTTTAGTCGTATCATTAGAAACATTTGACTTTATTCATAATTACTTCTAAAATCACGCTAACACGCGTGATTGATTGTGATGTAATGACGTTCAAAAATTGATGCCGTATCGAAATTAAACAATGCTAATAATGTACTCCATATGTTTGTAGATGGACAAGGCCTCGGTGTTGGGTGATGCTATAAAGTATGTGAAGGAGCTTAAGGAACAAGTAAAACTGTTGGAGGAGCAAAGCAAAAGCAAAAGTGTAGAACCGGTTACGGTTGTGAAGAAATTGTCTGAACTGTCTGCGGATGTCAATGTTTCGGACACTTCCTCAAATTCATGTAATGGAAACTCTGATGAAACATCAAAAACAAATCTGTCACTACCTGAAGTTAAAGCTAGTTTGTCGGGGAAGAATGTGCTGATTAGGATCCTCTGTGAGAAAGATAAGGCAGTGATGGGGAATGTATATAGAGAAATAGAGAAACTTGGTCTCTTGGTTATCAATGCTAGTTCGTTTTCATTTGGTTCCTCTGCTCTGGCCATAACCATCATTGCTCAGGTACATTTTTAATTAAGGACTATATGGTTATGATAATGATACTACTTTCGATAAATAAAAATGTCATAAAATGTGTTTAGCAGGAGAGGGATATGGTTTCCCTTCTGTTGGATTGCATCCATTCATGATGTTTGCGTATTTCTGGTTGTTTGATCAAGATTGAACGATTCAAATTTTAAACTTAATATTTTGGATTTTACGAAAATATTTTTTTACTGTCCGATCTTAATGCAATTTTTATTGATGTGTTGACTAGGAATACAAAAAAACGTGAGAGATGGAAATCAATTTCTTAGCGGGAGGAACCTTGCATGCTAATTAAAAACAGTTGACCAACATAATGTCTTAAGAGTGTTTTTTGTTAAAATGTAGTGTCAAATTGACTTGGTTGCTTAAAGTTCAATGTAATATTACTCGGTGTTTTTAGTTTTTCGTAAGGATCCAATAATGGTATCATGGTCAATAGTTTGATTGAGTCCTTATACCATTTTTTTAACAATGTGTTGTGGTCAATTGGTGTGTCTCGTTGATGTTGTATTAACGGTGTTGCAAATGAATGTGAATGAAAAAGTTTTAGTTTGCAAGGAAATCATATACTTAGATGAGTGAATGGACTCACACTTGAAGGAGAGATAGATTGTTAAGTGTGAGTAGTAATTTCATATTAAAAAATGGATTTGAACATCACAAAAGTTAGAGGACTCATATACGATCGATGTATTAATGCTTTGGGTAATGGTAATGGTGGTAAGGAGAGCAATGTCTCATCATTTGTTGTGTATATTTTTTTATAAGAGAGTAATATCTCATCACTATGGACTCAGAATAATTGACGTAATTGTGTCTTTATGGTGTTTGGCAGATGGAGAATGAATTGAACATGAGCATCCAGAAACTGGCAAAAAAACTGAGAGTTGGACTGGCGCAATTGAATTAG
Protein sequence

>MS.gene46331.t1

MKVVPTLRSKTKVVNSKNGPRRVKNQESNKKNGSLSKSTTHHTPDHIIAERIRREKISQQFIALSALIPNLKKMDKASVLGDAIKYVKELKEQVKLLEEQSKSKSVEPVTVVKKLSELSADVNVSDTSSNSCNGNSDETSKTNLSLPEVKASLSGKNVLIRILCEKDKAVMGNVYREIEKLGLLVINASSFSFGSSALAITIIAQMENELNMSIQKLAKKLRVGLAQLN