AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46332

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46332.t1 MTR_0246s0020 92.147 382 28 1 1 382 1 380 0.0 655
MS.gene46332.t1 MTR_0246s0020 89.008 373 37 2 1 373 1 369 0.0 606
MS.gene46332.t1 MTR_6g047570 57.179 390 104 8 1 381 1 336 2.19e-134 387
MS.gene46332.t1 MTR_0011s0260 58.462 390 121 10 1 380 1 359 2.69e-126 368
MS.gene46332.t1 MTR_0011s0210 58.163 392 123 10 1 382 1 361 1.72e-125 366
MS.gene46332.t1 MTR_4g067010 52.835 388 105 10 1 379 1 319 1.34e-114 337
MS.gene46332.t1 MTR_4g098035 50.630 397 125 14 1 381 1 342 1.77e-108 322
MS.gene46332.t1 MTR_4g066460 53.077 390 113 10 1 381 1 329 1.04e-106 317
MS.gene46332.t1 MTR_4g066380 50.765 392 120 9 1 383 1 328 2.59e-104 311
MS.gene46332.t1 MTR_0246s0050 49.465 374 120 12 23 381 2 321 2.59e-99 298
MS.gene46332.t1 MTR_0250s0040 48.611 360 88 9 24 381 1 265 1.62e-90 273
MS.gene46332.t1 MTR_6g047550 57.091 275 73 7 1 265 1 240 3.73e-88 268
MS.gene46332.t1 MTR_5g014520 50.958 261 99 6 121 380 101 333 1.43e-67 217
MS.gene46332.t1 MTR_5g014560 50.206 243 93 7 147 380 84 307 3.33e-61 199
MS.gene46332.t1 MTR_2g010450 49.462 186 85 3 196 379 175 353 4.76e-54 182
MS.gene46332.t1 MTR_5g014600 36.340 377 179 13 13 376 6 334 8.15e-54 181
MS.gene46332.t1 MTR_4g009540 49.474 190 90 3 191 377 145 331 8.12e-53 179
MS.gene46332.t1 MTR_4g009110 40.520 269 122 5 116 377 113 350 3.36e-49 169
MS.gene46332.t1 MTR_2g104490 40.000 265 129 6 108 371 67 302 3.01e-48 166
MS.gene46332.t1 MTR_8g009020 38.971 272 126 8 111 372 77 318 1.94e-45 159
MS.gene46332.t1 MTR_2g104550 38.489 278 136 7 108 380 68 315 1.39e-44 156
MS.gene46332.t1 MTR_4g009540 49.704 169 79 3 191 356 145 310 1.40e-44 156
MS.gene46332.t1 MTR_4g092700 43.781 201 101 5 182 379 142 333 5.10e-44 155
MS.gene46332.t1 MTR_4g097940 46.316 190 88 6 185 371 142 320 3.39e-43 153
MS.gene46332.t1 MTR_4g097950 41.045 268 121 10 120 378 12 251 6.06e-43 150
MS.gene46332.t1 MTR_4g097920 44.737 190 91 6 185 371 127 305 1.04e-42 151
MS.gene46332.t1 MTR_4g097920 44.737 190 91 6 185 371 142 320 1.17e-42 152
MS.gene46332.t1 MTR_2g104490 38.800 250 123 6 108 356 67 287 9.02e-41 146
MS.gene46332.t1 MTR_5g014640 35.135 333 156 13 68 376 27 323 1.06e-39 144
MS.gene46332.t1 MTR_8g069740 38.049 205 114 4 153 355 72 265 3.03e-39 141
MS.gene46332.t1 MTR_2g104650 39.269 219 127 3 159 375 53 267 2.26e-38 139
MS.gene46332.t1 MTR_2g104560 37.443 219 111 7 167 378 27 226 3.69e-38 137
MS.gene46332.t1 MTR_7g080780 31.343 335 178 8 74 384 13 319 2.88e-37 137
MS.gene46332.t1 MTR_7g080780 31.343 335 178 8 74 384 26 332 3.14e-37 137
MS.gene46332.t1 MTR_2g104500 33.929 280 124 8 106 377 46 272 2.19e-32 123
MS.gene46332.t1 MTR_8g009010 33.216 283 121 7 95 372 48 267 1.20e-31 122
MS.gene46332.t1 MTR_2g104530 35.122 205 114 3 169 372 83 269 2.70e-31 120
MS.gene46332.t1 MTR_2g104590 34.071 226 109 7 161 375 26 222 3.77e-30 116
MS.gene46332.t1 MTR_4g009510 40.854 164 62 5 221 374 64 202 3.41e-27 107
MS.gene46332.t1 MTR_7g451650 38.650 163 66 4 221 374 57 194 2.63e-25 102
MS.gene46332.t1 MTR_5g014550 48.062 129 59 2 228 355 54 175 1.48e-24 100
MS.gene46332.t1 MTR_7g050350 37.580 157 64 4 227 374 1 132 1.47e-23 95.5
MS.gene46332.t1 MTR_7g451650 37.580 157 64 4 227 374 1 132 1.47e-23 95.5
MS.gene46332.t1 MTR_4g009420 37.037 162 62 4 221 374 65 194 1.92e-23 97.1
MS.gene46332.t1 MTR_1g072320 31.016 187 108 6 198 371 470 648 1.83e-17 84.7
MS.gene46332.t1 MTR_8g067280 33.537 164 95 3 190 341 448 609 2.66e-17 84.0
MS.gene46332.t1 MTR_7g451650 35.862 145 59 4 221 356 57 176 1.86e-16 77.4
MS.gene46332.t1 MTR_8g024790 29.333 150 88 4 198 341 291 428 1.26e-15 78.6
MS.gene46332.t1 MTR_5g030430 30.726 179 112 3 190 357 479 656 3.66e-15 77.4
MS.gene46332.t1 MTR_4g009420 33.784 148 58 4 221 360 65 180 2.83e-14 71.6
MS.gene46332.t1 MTR_8g027495 48.438 64 33 0 198 261 458 521 8.11e-14 73.2
MS.gene46332.t1 MTR_7g096350 48.529 68 29 1 190 251 341 408 2.12e-13 72.0
MS.gene46332.t1 MTR_1g112680 27.011 174 104 5 190 341 309 481 6.96e-13 70.5
MS.gene46332.t1 MTR_8g064700 26.036 169 112 5 191 353 237 398 1.08e-12 69.3
MS.gene46332.t1 MTR_8g024770 45.333 75 40 1 198 271 279 353 2.29e-12 68.6
MS.gene46332.t1 MTR_7g117670 28.302 159 83 2 198 348 402 537 2.77e-12 68.6
MS.gene46332.t1 MTR_3g498695 24.868 189 138 3 183 367 283 471 1.69e-11 65.9
MS.gene46332.t1 MTR_7g110810 46.875 64 34 0 187 250 201 264 4.64e-11 64.3
MS.gene46332.t1 MTR_1g019240 36.538 104 63 2 153 254 49 151 4.67e-11 63.2
MS.gene46332.t1 MTR_3g030700 26.404 178 126 4 180 355 178 352 6.90e-11 63.5
MS.gene46332.t1 MTR_3g498695 25.806 155 112 2 183 334 283 437 7.52e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46332.t1 AT4G37850 38.356 292 142 10 101 379 62 328 1.04e-49 170
MS.gene46332.t1 AT4G37850 38.356 292 142 10 101 379 62 328 1.04e-49 170
MS.gene46332.t1 AT2G22750 45.685 197 104 2 183 378 110 304 7.63e-44 154
MS.gene46332.t1 AT2G22750 36.675 379 184 15 7 378 3 332 7.88e-44 155
MS.gene46332.t1 AT2G22750 45.685 197 104 2 183 378 134 328 8.29e-44 155
MS.gene46332.t1 AT2G22750 45.685 197 103 3 183 378 110 303 1.94e-42 150
MS.gene46332.t1 AT2G22750 36.675 379 183 16 7 378 3 331 1.94e-42 151
MS.gene46332.t1 AT2G22760 41.538 195 104 5 186 376 107 295 9.79e-40 143
MS.gene46332.t1 AT2G22760 40.805 174 94 4 186 356 107 274 3.12e-33 125
MS.gene46332.t1 AT2G22770 33.553 304 161 10 82 374 44 317 8.79e-30 117
MS.gene46332.t1 AT4G17880 32.768 177 100 4 190 357 402 568 5.99e-16 79.7
MS.gene46332.t1 AT5G46760 27.179 195 115 4 190 360 401 592 8.11e-16 79.3
MS.gene46332.t1 AT1G32640 32.692 156 83 4 190 334 438 582 3.91e-15 77.4
MS.gene46332.t1 AT1G63650 30.464 151 88 4 198 334 405 552 9.51e-15 76.3
MS.gene46332.t1 AT1G63650 30.464 151 88 4 198 334 405 552 9.51e-15 76.3
MS.gene46332.t1 AT1G63650 30.464 151 88 4 198 334 405 552 9.51e-15 76.3
MS.gene46332.t1 AT1G01260 47.143 70 37 0 192 261 427 496 1.33e-14 75.9
MS.gene46332.t1 AT1G01260 47.143 70 37 0 192 261 427 496 1.33e-14 75.9
MS.gene46332.t1 AT1G01260 47.143 70 37 0 192 261 427 496 1.33e-14 75.9
MS.gene46332.t1 AT4G16430 50.746 67 33 0 192 258 314 380 1.34e-14 75.5
MS.gene46332.t1 AT2G46510 45.946 74 34 1 190 257 381 454 2.14e-14 75.1
MS.gene46332.t1 AT5G41315 25.628 199 121 6 192 369 435 627 1.08e-12 70.1
MS.gene46332.t1 AT5G41315 25.628 199 121 6 192 369 440 632 1.11e-12 70.1
MS.gene46332.t1 AT5G41315 25.628 199 121 6 192 369 443 635 1.26e-12 69.7
MS.gene46332.t1 AT4G09820 45.455 77 35 2 189 258 352 428 1.96e-12 68.9
MS.gene46332.t1 AT4G00870 32.283 127 64 2 199 316 250 363 5.24e-12 67.4
MS.gene46332.t1 AT2G16910 27.711 166 95 4 199 344 315 475 9.40e-12 67.0
MS.gene46332.t1 AT4G29930 26.163 172 113 4 199 356 55 226 1.12e-11 65.1
MS.gene46332.t1 AT4G29930 26.163 172 113 4 199 356 55 226 1.12e-11 65.1
MS.gene46332.t1 AT4G29930 26.437 174 114 4 199 358 55 228 1.14e-11 65.1
MS.gene46332.t1 AT5G57150 28.655 171 106 4 196 357 53 216 2.46e-11 63.5
MS.gene46332.t1 AT5G57150 28.824 170 105 4 196 356 53 215 4.00e-11 62.8
MS.gene46332.t1 AT5G57150 29.070 172 106 4 196 358 52 216 4.28e-11 63.2
MS.gene46332.t1 AT3G06120 24.339 189 118 5 198 367 2 184 4.52e-11 62.4
MS.gene46332.t1 AT5G57150 28.824 170 105 4 196 356 53 215 4.52e-11 63.2
MS.gene46332.t1 AT5G57150 28.824 170 105 4 196 356 52 214 4.66e-11 62.8

Find 65 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8.4:-24598040 None:intergenic
GTGTTGGTAGGAGGAGAATT+TGG 0.206656 8.4:-24597200 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8.4:+24596768 MS.gene46332:CDS
GACATGAATGTTTCTCATTT+GGG 0.281293 8.4:-24597297 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8.4:-24597298 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8.4:-24596920 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8.4:-24596906 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8.4:+24598040 MS.gene46332:CDS
TGTTGGTAGGAGGAGAATTT+GGG 0.316269 8.4:-24597199 None:intergenic
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8.4:+24597472 MS.gene46332:CDS
GTTTCTTCTGATACGTTCAT+TGG 0.319465 8.4:-24596745 None:intergenic
CATCATCATCAATGATATTA+TGG 0.325312 8.4:-24597902 None:intergenic
CAGGAGTGAGTCCACAAAAC+TGG 0.341350 8.4:-24597226 None:intergenic
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8.4:+24598108 MS.gene46332:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8.4:-24596767 None:intergenic
GGCGTTGGCTCGAGACCTTT+TGG 0.371700 8.4:-24597381 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8.4:-24597134 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8.4:+24598107 MS.gene46332:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8.4:-24597409 None:intergenic
CTGAACTCAAACATTGTTGT+TGG 0.389224 8.4:-24596971 None:intergenic
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8.4:-24597448 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8.4:+24597317 MS.gene46332:CDS
GTTGGTGATAGTTGTGGAAT+TGG 0.425475 8.4:-24596997 None:intergenic
TGAACTCAAACATTGTTGTT+GGG 0.428381 8.4:-24596970 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8.4:+24598009 MS.gene46332:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8.4:+24597990 MS.gene46332:CDS
AGGAGTGAGTCCACAAAACT+GGG 0.451243 8.4:-24597225 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8.4:+24597318 MS.gene46332:CDS
TCATATGATGAGAGTATTGA+AGG 0.466240 8.4:+24597948 MS.gene46332:CDS
TGTTGTTGGGTTTGTTCTTG+TGG 0.480560 8.4:-24596957 None:intergenic
TGACATTACTATCATTGCTC+AGG 0.494765 8.4:+24598139 MS.gene46332:CDS
AGCAATGATAGTAATGTCAA+TGG 0.495292 8.4:-24598135 None:intergenic
TTTGACTTTGAGTTTAAGGC+AGG 0.501893 8.4:-24597245 None:intergenic
TCATCAATGATATTATGGTC+TGG 0.514204 8.4:-24597897 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8.4:-24597402 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8.4:-24597396 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8.4:+24596923 MS.gene46332:CDS
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8.4:-24596929 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8.4:+24596755 MS.gene46332:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8.4:-24597293 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8.4:+24597397 MS.gene46332:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8.4:-24597146 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8.4:+24598109 MS.gene46332:CDS
TAAAATTTGGTAGATGGACA+AGG 0.590351 8.4:+24597761 MS.gene46332:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8.4:-24598122 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8.4:+24599115 MS.gene46332:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8.4:-24599151 None:intergenic
TGAAATTGAGAGACCAACCA+TGG 0.603140 8.4:+24597064 MS.gene46332:CDS
TGGTAGGAGGAGAATTTGGG+AGG 0.603292 8.4:-24597196 None:intergenic
GAATGAGTTGGTGATAGTTG+TGG 0.603767 8.4:-24597003 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8.4:+24598052 MS.gene46332:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8.4:+24598031 MS.gene46332:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8.4:+24598033 MS.gene46332:CDS
AATGTCAATGGAATCCCCAA+AGG 0.614554 8.4:-24598123 None:intergenic
TGTTTCATCAGTGAATGAGT+TGG 0.619624 8.4:-24597015 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8.4:-24597296 None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8.4:+24597991 MS.gene46332:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8.4:+24597860 MS.gene46332:CDS
GAGTGAGTCCACAAAACTGG+GGG 0.649560 8.4:-24597223 None:intergenic
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8.4:-24597877 None:intergenic
GGAGTGAGTCCACAAAACTG+GGG 0.660208 8.4:-24597224 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8.4:+24598032 MS.gene46332:CDS
AGAACAAAACAAGAAAACAA+AGG 0.679620 8.4:+24597848 MS.gene46332:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8.4:+24599139 MS.gene46332:CDS
AGTGAGTCCACAAAACTGGG+GGG 0.773710 8.4:-24597222 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAATTTATTATCATAAA+AGG + chr8.4:24598342-24598361 MS.gene46332:intron 15.0%
!! CAAGTATAGAAATAATTTAT+TGG + chr8.4:24598698-24598717 MS.gene46332:intron 15.0%
!! TCAAATACTGTTATAAAATT+TGG + chr8.4:24597748-24597767 MS.gene46332:intron 15.0%
!! TTTAAACAATGAATATTTAG+TGG - chr8.4:24598197-24598216 None:intergenic 15.0%
!!! AAACTTGTGTTTTTTTTTTT+TGG + chr8.4:24598635-24598654 MS.gene46332:intron 15.0%
!!! AACTTGTGTTTTTTTTTTTT+GGG + chr8.4:24598636-24598655 MS.gene46332:intron 15.0%
!!! AAGTATTATTATTTTGTTTG+AGG + chr8.4:24598252-24598271 MS.gene46332:intron 15.0%
!!! ATTAAATGCACAAAATTTTA+AGG + chr8.4:24598759-24598778 MS.gene46332:intron 15.0%
!!! ATTATTACATTTTTTTGTCT+AGG + chr8.4:24598486-24598505 MS.gene46332:intron 15.0%
!!! TTATTACATTTTTTTGTCTA+GGG + chr8.4:24598487-24598506 MS.gene46332:intron 15.0%
!! ATAATTAATGAAATGCTAAC+CGG - chr8.4:24598459-24598478 None:intergenic 20.0%
!! ATATTTAGTGGCAATTATTA+AGG - chr8.4:24598185-24598204 None:intergenic 20.0%
!!! AACTTGTTAAGCATAAAAAA+AGG + chr8.4:24598323-24598342 MS.gene46332:intron 20.0%
!!! ACACTACTTTTTTTTATATC+AGG + chr8.4:24598832-24598851 MS.gene46332:intron 20.0%
!!! ATTGTTATACTTTTGTAGAT+GGG + chr8.4:24599089-24599108 MS.gene46332:intron 20.0%
!!! ATTTTTTTTTTTGTTGTTGC+AGG + chr8.4:24596860-24596879 MS.gene46332:intron 20.0%
!!! TATTACATTTTTTTGTCTAG+GGG + chr8.4:24598488-24598507 MS.gene46332:intron 20.0%
!!! TATTGTTATACTTTTGTAGA+TGG + chr8.4:24599088-24599107 MS.gene46332:intron 20.0%
!!! TCTTTTTAGGAATTTTGTTT+AGG + chr8.4:24598996-24599015 MS.gene46332:intron 20.0%
!!! TGATTTGGTAAGAAAATTAA+AGG + chr8.4:24596783-24596802 MS.gene46332:intron 20.0%
!!! TTAAGGAATATTTCTTCTTT+TGG + chr8.4:24598776-24598795 MS.gene46332:intron 20.0%
!!! TTTTCTATCTGTTTCTTTTT+AGG + chr8.4:24598983-24599002 MS.gene46332:intron 20.0%
! AATACAATTAAACAACTCGT+GGG - chr8.4:24599061-24599080 None:intergenic 25.0%
! AGAACAAAACAAGAAAACAA+AGG + chr8.4:24597848-24597867 MS.gene46332:CDS 25.0%
! AGTAGTGAAAAAATGTTTAG+TGG - chr8.4:24598228-24598247 None:intergenic 25.0%
! ATTACACGTATTCATGAAAA+GGG + chr8.4:24598937-24598956 MS.gene46332:intron 25.0%
! CATCATCATCAATGATATTA+TGG - chr8.4:24597905-24597924 None:intergenic 25.0%
! CTGTTATAAAATTTGGTAGA+TGG + chr8.4:24597755-24597774 MS.gene46332:intron 25.0%
! TAATACAATTAAACAACTCG+TGG - chr8.4:24599062-24599081 None:intergenic 25.0%
! TAATTAATGAAATGCTAACC+GGG - chr8.4:24598458-24598477 None:intergenic 25.0%
! TATTACACGTATTCATGAAA+AGG + chr8.4:24598936-24598955 MS.gene46332:intron 25.0%
!! TGTTGGCTGTTATAATTAAT+TGG - chr8.4:24598891-24598910 None:intergenic 25.0%
!!! AAAGTATCGTCAATAATTGT+TGG - chr8.4:24598908-24598927 None:intergenic 25.0%
!!! AATAATTAACTTGTGCCTTA+AGG - chr8.4:24598678-24598697 None:intergenic 25.0%
!!! ATAATTAACTTGTGCCTTAA+GGG - chr8.4:24598677-24598696 None:intergenic 25.0%
AAAGATGAGTTAGATGATGA+TGG - chr8.4:24597149-24597168 None:intergenic 30.0%
AATATCATTAATCAATGCCC+CGG + chr8.4:24598437-24598456 MS.gene46332:intron 30.0%
AATTAATGAAATGCTAACCG+GGG - chr8.4:24598457-24598476 None:intergenic 30.0%
ACATGAATGTTTCTCATTTG+GGG - chr8.4:24597299-24597318 None:intergenic 30.0%
AGACATGAATGTTTCTCATT+TGG - chr8.4:24597301-24597320 None:intergenic 30.0%
AGCAATGATAGTAATGTCAA+TGG - chr8.4:24598138-24598157 None:intergenic 30.0%
CAAAAACATCTCAAACCAAA+AGG + chr8.4:24597366-24597385 MS.gene46332:CDS 30.0%
GACATGAATGTTTCTCATTT+GGG - chr8.4:24597300-24597319 None:intergenic 30.0%
TAAAATTTGGTAGATGGACA+AGG + chr8.4:24597761-24597780 MS.gene46332:intron 30.0%
TCATCAATGATATTATGGTC+TGG - chr8.4:24597900-24597919 None:intergenic 30.0%
TCTCAACTCAAAATCCTAAA+GGG + chr8.4:24597318-24597337 MS.gene46332:CDS 30.0%
TTAGTGGCAATTATTAAGGT+AGG - chr8.4:24598181-24598200 None:intergenic 30.0%
! TGAACTCAAACATTGTTGTT+GGG - chr8.4:24596973-24596992 None:intergenic 30.0%
!! TCATATGATGAGAGTATTGA+AGG + chr8.4:24597948-24597967 MS.gene46332:CDS 30.0%
!!! AACATTGATTTTGAAAGCGA+GGG - chr8.4:24598535-24598554 None:intergenic 30.0%
!!! ACTTTTGACTCTTTTGACTT+TGG + chr8.4:24597038-24597057 MS.gene46332:CDS 30.0%
!!! ATTTTTTAACATGTGCCCTA+AGG + chr8.4:24598799-24598818 MS.gene46332:intron 30.0%
!!! CTGTTTTGACTTTGAGTTTA+AGG - chr8.4:24597252-24597271 None:intergenic 30.0%
!!! TAAAAAGCGTTTGGAAGTTT+TGG + chr8.4:24597824-24597843 MS.gene46332:CDS 30.0%
!!! TGTTGCAAAGTTTTGAGTTT+TGG - chr8.4:24597348-24597367 None:intergenic 30.0%
!!! TTTTTTAACATGTGCCCTAA+GGG + chr8.4:24598800-24598819 MS.gene46332:intron 30.0%
!!! TTTTTTGTTGTTGCAGGAAA+TGG + chr8.4:24596866-24596885 MS.gene46332:intron 30.0%
AAACTTCCTTGATGCTAATG+AGG + chr8.4:24596923-24596942 MS.gene46332:CDS 35.0%
AATGTGTATTAGTGTCGTGT+TGG - chr8.4:24597553-24597572 None:intergenic 35.0%
ATTATTAGGGTCATGCTAAC+CGG + chr8.4:24598290-24598309 MS.gene46332:intron 35.0%
CACTTCACACTTATGTTTGA+AGG + chr8.4:24597498-24597517 MS.gene46332:intron 35.0%
CATTAGCATCAAGGAAGTTT+AGG - chr8.4:24596923-24596942 None:intergenic 35.0%
CTGAACTCAAACATTGTTGT+TGG - chr8.4:24596974-24596993 None:intergenic 35.0%
GTCTCAACTCAAAATCCTAA+AGG + chr8.4:24597317-24597336 MS.gene46332:CDS 35.0%
GTTTCTTCTGATACGTTCAT+TGG - chr8.4:24596748-24596767 None:intergenic 35.0%
TAACAAGTTTCTCAGAGTAC+CGG - chr8.4:24598312-24598331 None:intergenic 35.0%
TACTATCATTGCTCAGGTAA+AGG + chr8.4:24598145-24598164 MS.gene46332:intron 35.0%
TATCAGAAGAAACCAGCAAA+TGG + chr8.4:24596755-24596774 MS.gene46332:CDS 35.0%
TCTTGTTCCTAACCTGAAAA+AGG + chr8.4:24597472-24597491 MS.gene46332:CDS 35.0%
TGACATTACTATCATTGCTC+AGG + chr8.4:24598139-24598158 MS.gene46332:CDS 35.0%
TGTTTCATCAGTGAATGAGT+TGG - chr8.4:24597018-24597037 None:intergenic 35.0%
! AACAATTGTTTTCAGCTCCA+TGG - chr8.4:24597084-24597103 None:intergenic 35.0%
! ATACTTTTGTAGATGGGTGA+AGG + chr8.4:24599095-24599114 MS.gene46332:intron 35.0%
! TACTTTTGTAGATGGGTGAA+GGG + chr8.4:24599096-24599115 MS.gene46332:intron 35.0%
! TTTGACTTTGAGTTTAAGGC+AGG - chr8.4:24597248-24597267 None:intergenic 35.0%
!! AAGTTTAGGTTGCATTCTTC+AGG - chr8.4:24596909-24596928 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG + chr8.4:24598660-24598679 MS.gene46332:intron 35.0%
!! GAAAGAGCTTAAAAAGCGTT+TGG + chr8.4:24597815-24597834 MS.gene46332:CDS 35.0%
!! TGGAGAAAGTTTTGGTGAAA+TGG - chr8.4:24597129-24597148 None:intergenic 35.0%
!! TGTGAAGTGTTACCTTTTTC+AGG - chr8.4:24597487-24597506 None:intergenic 35.0%
!! TTGAGTGCTATTTAGCTAGT+GGG - chr8.4:24597683-24597702 None:intergenic 35.0%
!! TTTGAGTGCTATTTAGCTAG+TGG - chr8.4:24597684-24597703 None:intergenic 35.0%
!!! AGTGTTACCTTTTTCAGGTT+AGG - chr8.4:24597482-24597501 None:intergenic 35.0%
!!! CAACATTGATTTTGAAAGCG+AGG - chr8.4:24598536-24598555 None:intergenic 35.0%
!!! GATGATGATGGAGAAAGTTT+TGG - chr8.4:24597137-24597156 None:intergenic 35.0%
!!! TGAGTTTTGGATAACCCTTT+AGG - chr8.4:24597335-24597354 None:intergenic 35.0%
AATAGCAGTGTCTTACCCTT+TGG + chr8.4:24598107-24598126 MS.gene46332:CDS 40.0%
AATGTCAATGGAATCCCCAA+AGG - chr8.4:24598126-24598145 None:intergenic 40.0%
ACACGTATTCATGAAAAGGG+AGG + chr8.4:24598940-24598959 MS.gene46332:intron 40.0%
ACTCTTCCTCATTAGCATCA+AGG - chr8.4:24596932-24596951 None:intergenic 40.0%
AGGGTACAACTTGAGCATAA+AGG + chr8.4:24599115-24599134 MS.gene46332:CDS 40.0%
ATAGCAGTGTCTTACCCTTT+GGG + chr8.4:24598108-24598127 MS.gene46332:CDS 40.0%
ATGTCAATGGAATCCCCAAA+GGG - chr8.4:24598125-24598144 None:intergenic 40.0%
CAAATCAGAAAGCCATTTGC+TGG - chr8.4:24596770-24596789 None:intergenic 40.0%
CACGTATTCATGAAAAGGGA+GGG + chr8.4:24598941-24598960 MS.gene46332:intron 40.0%
CAGCAAATGGCTTTCTGATT+TGG + chr8.4:24596768-24596787 MS.gene46332:CDS 40.0%
GCTTTCTTTCAGCCATGATA+TGG - chr8.4:24597412-24597431 None:intergenic 40.0%
TGAAATTGAGAGACCAACCA+TGG + chr8.4:24597064-24597083 MS.gene46332:CDS 40.0%
TGAATGTTTCTCATTTGGGG+TGG - chr8.4:24597296-24597315 None:intergenic 40.0%
TGGTTTCTTCAGAACAACCA+CGG - chr8.4:24597880-24597899 None:intergenic 40.0%
TTACTGTCGATGTTGTGTCT+TGG + chr8.4:24597622-24597641 MS.gene46332:intron 40.0%
TTCAGCCATGATATGGTCAT+CGG - chr8.4:24597405-24597424 None:intergenic 40.0%
! ATTGTTTTCAGCTCCATGGT+TGG - chr8.4:24597080-24597099 None:intergenic 40.0%
! CACTTTTGCTTGCACTTGAA+AGG - chr8.4:24597988-24598007 None:intergenic 40.0%
! TGTTGGTAGGAGGAGAATTT+GGG - chr8.4:24597202-24597221 None:intergenic 40.0%
! TGTTGTTGGGTTTGTTCTTG+TGG - chr8.4:24596960-24596979 None:intergenic 40.0%
!! AAGTGCAAGCAAAAGTGTCA+GGG + chr8.4:24597991-24598010 MS.gene46332:CDS 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG - chr8.4:24598817-24598836 None:intergenic 40.0%
!! GAATGAGTTGGTGATAGTTG+TGG - chr8.4:24597006-24597025 None:intergenic 40.0%
!! GTTGGTGATAGTTGTGGAAT+TGG - chr8.4:24597000-24597019 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG - chr8.4:24598818-24598837 None:intergenic 40.0%
!! TGAGTGCTATTTAGCTAGTG+GGG - chr8.4:24597682-24597701 None:intergenic 40.0%
ACTTGTCAAGAACCTACGCA+TGG + chr8.4:24599139-24599158 MS.gene46332:CDS 45.0%
AGGAGTGAGTCCACAAAACT+GGG - chr8.4:24597228-24597247 None:intergenic 45.0%
CTGCAAGAGCAATGAAGCTT+TGG - chr8.4:24597451-24597470 None:intergenic 45.0%
GAAAACAAAGGTAGAGTCCG+TGG + chr8.4:24597860-24597879 MS.gene46332:CDS 45.0%
GGTCATCTGAGTGACACATT+TGG - chr8.4:24598514-24598533 None:intergenic 45.0%
TAGCAGTGTCTTACCCTTTG+GGG + chr8.4:24598109-24598128 MS.gene46332:CDS 45.0%
! ATTCACTGCCAGAAGCACAA+GGG + chr8.4:24598032-24598051 MS.gene46332:CDS 45.0%
! CAGGGAAAGAGATGCTGATT+CGG + chr8.4:24598009-24598028 MS.gene46332:CDS 45.0%
! GTGTTGGTAGGAGGAGAATT+TGG - chr8.4:24597203-24597222 None:intergenic 45.0%
!! CAAGTGCAAGCAAAAGTGTC+AGG + chr8.4:24597990-24598009 MS.gene46332:CDS 45.0%
!! GGGAGGGCGTATTTTATTCT+AGG + chr8.4:24598957-24598976 MS.gene46332:intron 45.0%
!! ACTAATTATTATTAATTATT+AGG + chr8.4:24598276-24598295 MS.gene46332:intron 5.0%
!! CTAATTATTATTAATTATTA+GGG + chr8.4:24598277-24598296 MS.gene46332:intron 5.0%
ACTTCAATGCTGCCATGCGT+AGG - chr8.4:24599154-24599173 None:intergenic 50.0%
AGTGAGTCCACAAAACTGGG+GGG - chr8.4:24597225-24597244 None:intergenic 50.0%
CAACGCCGATGACCATATCA+TGG + chr8.4:24597397-24597416 MS.gene46332:CDS 50.0%
CAGGAGTGAGTCCACAAAAC+TGG - chr8.4:24597229-24597248 None:intergenic 50.0%
CATGATATGGTCATCGGCGT+TGG - chr8.4:24597399-24597418 None:intergenic 50.0%
GAGTGAGTCCACAAAACTGG+GGG - chr8.4:24597226-24597245 None:intergenic 50.0%
GGAGTGAGTCCACAAAACTG+GGG - chr8.4:24597227-24597246 None:intergenic 50.0%
GGGGATTCTGGTGAAAGTCA+TGG + chr8.4:24598052-24598071 MS.gene46332:CDS 50.0%
TGGTAGGAGGAGAATTTGGG+AGG - chr8.4:24597199-24597218 None:intergenic 50.0%
! GATTCACTGCCAGAAGCACA+AGG + chr8.4:24598031-24598050 MS.gene46332:CDS 50.0%
! TTCACTGCCAGAAGCACAAG+GGG + chr8.4:24598033-24598052 MS.gene46332:CDS 50.0%
CCAGAATCCCCTTGTGCTTC+TGG - chr8.4:24598043-24598062 None:intergenic 55.0%
! ACCAACACCCCCCAGTTTTG+TGG + chr8.4:24597215-24597234 MS.gene46332:CDS 55.0%
! CCAGAAGCACAAGGGGATTC+TGG + chr8.4:24598040-24598059 MS.gene46332:CDS 55.0%
! TCCACAAAACTGGGGGGTGT+TGG - chr8.4:24597219-24597238 None:intergenic 55.0%
!! CAAAACTGGGGGGTGTTGGT+AGG - chr8.4:24597215-24597234 None:intergenic 55.0%
!! AACTGGGGGGTGTTGGTAGG+AGG - chr8.4:24597212-24597231 None:intergenic 60.0%
!!! GGCGTTGGCTCGAGACCTTT+TGG - chr8.4:24597384-24597403 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 24596724 24599196 24596724 ID=MS.gene46332
chr8.4 mRNA 24596724 24599196 24596724 ID=MS.gene46332.t1;Parent=MS.gene46332
chr8.4 exon 24596724 24596789 24596724 ID=MS.gene46332.t1.exon1;Parent=MS.gene46332.t1
chr8.4 CDS 24596724 24596789 24596724 ID=cds.MS.gene46332.t1;Parent=MS.gene46332.t1
chr8.4 exon 24596882 24597493 24596882 ID=MS.gene46332.t1.exon2;Parent=MS.gene46332.t1
chr8.4 CDS 24596882 24597493 24596882 ID=cds.MS.gene46332.t1;Parent=MS.gene46332.t1
chr8.4 exon 24597774 24598160 24597774 ID=MS.gene46332.t1.exon3;Parent=MS.gene46332.t1
chr8.4 CDS 24597774 24598160 24597774 ID=cds.MS.gene46332.t1;Parent=MS.gene46332.t1
chr8.4 exon 24599107 24599196 24599107 ID=MS.gene46332.t1.exon4;Parent=MS.gene46332.t1
chr8.4 CDS 24599107 24599196 24599107 ID=cds.MS.gene46332.t1;Parent=MS.gene46332.t1
Gene Sequence

>MS.gene46332

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGTAAGAAAATTAAAGGCAAATTAACATATTATTGATTATATAAGTAAATTCTTTATCATTTTAAATTTTAATTTTTTTTTTTGTTGTTGCAGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAATATCATTGATGATGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCTTACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTCATGCTAACCGGTACTCTGAGAAACTTGTTAAGCATAAAAAAAGGAATTTATTATCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTTGTCTAGGGGCCAAATGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTTTTTTTTTTTGGGAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTCAAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAAATTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATATTTCTTCTTTTGGATTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTATATCAGGATATTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>MS.gene46332.t1

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTFDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHNIIDDDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSLQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE