AlfalfaGEDB | MS.gene46367

M. sativa cultivar XinJiangDaYe / MS.gene46367

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46367.t1	RHN42229.1	78.3	69	15	0	1	69	19	87	2.10E-22	114.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46367.t1	Q32SG3	48.3	60	31	0	9	68	34	93	4.6e-10	64.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46367.t1	G7LGC9	78.3	69	15	0	1	69	1	69	1.5e-22	114.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene46367.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46367.t1	MTR_8g079660	78.261	69	15	0	1	69	1	69	4.15e-34	117
MS.gene46367.t1	MTR_8g079620	65.217	69	24	0	1	69	1	69	4.14e-26	95.5
MS.gene46367.t1	MTR_8g079580	54.286	70	31	1	1	69	1	70	2.40e-19	77.8
MS.gene46367.t1	MTR_3g452660	53.333	60	28	0	9	68	8	67	4.16e-17	72.8
MS.gene46367.t1	MTR_5g017950	46.667	60	32	0	9	68	9	68	3.57e-15	67.8
MS.gene46367.t1	MTR_3g073690	47.541	61	32	0	9	69	10	70	6.64e-15	65.9
MS.gene46367.t1	MTR_4g099230	44.444	63	34	1	8	69	2	64	1.21e-13	61.6
MS.gene46367.t1	MTR_8g040900	46.667	60	32	0	9	68	8	67	2.31e-13	62.0
MS.gene46367.t1	MTR_5g093010	44.615	65	36	0	5	69	2	66	4.55e-13	59.7
MS.gene46367.t1	MTR_7g033800	45.902	61	33	0	9	69	12	72	4.77e-13	60.5
MS.gene46367.t1	MTR_5g083230	47.541	61	32	0	9	69	7	67	5.15e-13	60.1
MS.gene46367.t1	MTR_4g105170	45.902	61	33	0	9	69	12	72	6.73e-13	60.1
MS.gene46367.t1	MTR_3g077240	45.902	61	33	0	9	69	11	71	1.33e-12	59.7
MS.gene46367.t1	MTR_3g031660	42.623	61	35	0	9	69	8	68	1.91e-12	58.9
MS.gene46367.t1	MTR_6g005080	42.623	61	35	0	9	69	11	71	2.40e-12	57.8
MS.gene46367.t1	MTR_3g071590	44.262	61	34	0	9	69	9	69	3.23e-12	58.2
MS.gene46367.t1	MTR_7g028905	42.188	64	37	0	5	68	2	65	6.75e-12	56.2
MS.gene46367.t1	MTR_2g093310	38.806	67	41	0	3	69	28	94	1.00e-11	57.4
MS.gene46367.t1	MTR_6g005070	42.623	61	35	0	9	69	10	70	1.38e-11	56.2
MS.gene46367.t1	MTR_8g017090	37.705	61	38	0	9	69	13	73	4.14e-11	56.6
MS.gene46367.t1	MTR_6g027700	41.935	62	35	1	9	69	8	69	8.28e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46367.t1	AT3G26660	48.333	60	31	0	9	68	6	65	2.49e-15	65.1
MS.gene46367.t1	AT3G26620	48.333	60	31	0	9	68	6	65	2.83e-15	65.1
MS.gene46367.t1	AT1G65620	47.541	61	32	0	9	69	10	70	2.74e-14	64.3
MS.gene46367.t1	AT1G65620	47.541	61	32	0	9	69	10	70	2.74e-14	64.3
MS.gene46367.t1	AT1G65620	47.541	61	32	0	9	69	10	70	2.74e-14	64.3
MS.gene46367.t1	AT1G65620	47.541	61	32	0	9	69	10	70	2.74e-14	64.3
MS.gene46367.t1	AT1G65620	47.541	61	32	0	9	69	10	70	2.74e-14	64.3
MS.gene46367.t1	AT2G23660	48.333	60	31	0	9	68	6	65	3.74e-14	65.1
MS.gene46367.t1	AT2G23660	48.333	60	31	0	9	68	6	65	3.74e-14	65.1
MS.gene46367.t1	AT2G23660	48.333	60	31	0	9	68	6	65	3.74e-14	65.1
MS.gene46367.t1	AT2G30130	47.541	61	32	0	9	69	9	69	3.82e-13	61.2
MS.gene46367.t1	AT3G27650	45.902	61	33	0	9	69	40	100	4.12e-13	60.5
MS.gene46367.t1	AT5G66870	46.667	60	32	0	9	68	8	67	4.87e-13	62.0
MS.gene46367.t1	AT1G72980	39.344	61	37	0	9	69	14	74	2.70e-12	58.9
MS.gene46367.t1	AT1G07900	40.299	67	40	0	3	69	28	94	3.49e-12	58.5
MS.gene46367.t1	AT1G31320	42.623	61	35	0	9	69	14	74	4.85e-12	57.8
MS.gene46367.t1	AT2G28500	40.000	65	39	0	5	69	52	116	5.80e-12	58.5
MS.gene46367.t1	AT5G63090	44.262	61	34	0	9	69	12	72	8.89e-12	57.4
MS.gene46367.t1	AT5G63090	44.262	61	34	0	9	69	12	72	8.89e-12	57.4
MS.gene46367.t1	AT5G63090	44.262	61	34	0	9	69	12	72	8.89e-12	57.4
MS.gene46367.t1	AT5G63090	44.262	61	34	0	9	69	12	72	8.89e-12	57.4
MS.gene46367.t1	AT1G16530	43.548	62	34	1	9	69	15	76	5.09e-11	55.1
MS.gene46367.t1	AT2G40470	43.077	65	37	0	5	69	17	81	6.08e-11	55.5
MS.gene46367.t1	AT2G40470	44.262	61	34	0	9	69	46	106	6.53e-11	55.5
MS.gene46367.t1	AT3G47870	39.344	61	37	0	9	69	37	97	8.60e-11	55.8

Find 16 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAAGGTACACTCCATGTT+TGG	0.353035	8.4:-24066109	MS.gene46367:CDS
AGGTTGTTGGGTGGAATGTT+AGG	0.369691	8.4:+24066142	None:intergenic
GACGGAGATGCTTGCACGCT+GGG	0.417388	8.4:+24066194	None:intergenic
GATGAAATGTTGTAGGTTGT+TGG	0.421361	8.4:+24066129	None:intergenic
ATGAAATGTTGTAGGTTGTT+GGG	0.433562	8.4:+24066130	None:intergenic
TGAACAAGCTATTGAATCCT+TGG	0.435375	8.4:-24066038	MS.gene46367:CDS
CACATTGTTCTTTCCAAACA+TGG	0.438190	8.4:+24066096	None:intergenic
GTACCTTGATGAAATGTTGT+AGG	0.450654	8.4:+24066122	None:intergenic
CGACGGAGATGCTTGCACGC+TGG	0.511634	8.4:+24066193	None:intergenic
CAGCGTGCAAGCATCTCCGT+CGG	0.548053	8.4:-24066192	MS.gene46367:CDS
AGCGTGCAAGCATCTCCGTC+GGG	0.559116	8.4:-24066191	MS.gene46367:CDS
AAATGTTGTAGGTTGTTGGG+TGG	0.588074	8.4:+24066133	None:intergenic
CAACCTACAACATTTCATCA+AGG	0.604500	8.4:-24066125	MS.gene46367:CDS
TGGGTGGAATGTTAGGAGCA+AGG	0.620513	8.4:+24066149	None:intergenic
AACTTGAGTCACACTCCCGA+CGG	0.656492	8.4:+24066176	None:intergenic
AGGCTCAGGCACTTACACCA+AGG	0.698849	8.4:+24066021	None:intergenic

CRISPR-GE

sgRNA_sequence	Strand	Position	Region	GC_content
ATGAAATGTTGTAGGTTGTT+GGG	+	chr8.4:24066126-24066145	None:intergenic	30.0%
CAACCTACAACATTTCATCA+AGG	-	chr8.4:24066128-24066147	MS.gene46367:CDS	35.0%
CACATTGTTCTTTCCAAACA+TGG	+	chr8.4:24066160-24066179	None:intergenic	35.0%
GATGAAATGTTGTAGGTTGT+TGG	+	chr8.4:24066127-24066146	None:intergenic	35.0%
GTACCTTGATGAAATGTTGT+AGG	+	chr8.4:24066134-24066153	None:intergenic	35.0%
TGAACAAGCTATTGAATCCT+TGG	-	chr8.4:24066215-24066234	MS.gene46367:CDS	35.0%
AAATGTTGTAGGTTGTTGGG+TGG	+	chr8.4:24066123-24066142	None:intergenic	40.0%
ATCAAGGTACACTCCATGTT+TGG	-	chr8.4:24066144-24066163	MS.gene46367:CDS	40.0%
AGGTTGTTGGGTGGAATGTT+AGG	+	chr8.4:24066114-24066133	None:intergenic	45.0%
AACTTGAGTCACACTCCCGA+CGG	+	chr8.4:24066080-24066099	None:intergenic	50.0%
TGGGTGGAATGTTAGGAGCA+AGG	+	chr8.4:24066107-24066126	None:intergenic	50.0%
AGCGTGCAAGCATCTCCGTC+GGG	-	chr8.4:24066062-24066081	MS.gene46367:CDS	60.0%
CAGCGTGCAAGCATCTCCGT+CGG	-	chr8.4:24066061-24066080	MS.gene46367:CDS	60.0%
GACGGAGATGCTTGCACGCT+GGG	+	chr8.4:24066062-24066081	None:intergenic	60.0%
CGACGGAGATGCTTGCACGC+TGG	+	chr8.4:24066063-24066082	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	24066033	24066242	24066033	ID=MS.gene46367
chr8.4	mRNA	24066033	24066242	24066033	ID=MS.gene46367.t1;Parent=MS.gene46367
chr8.4	exon	24066033	24066242	24066033	ID=MS.gene46367.t1.exon1;Parent=MS.gene46367.t1
chr8.4	CDS	24066033	24066242	24066033	ID=cds.MS.gene46367.t1;Parent=MS.gene46367.t1

Gene Sequence	>MS.gene46367 ATGGCAAGAGCAATAATTCGTCTGTGCCCAGCGTGCAAGCATCTCCGTCGGGAGTGTGACTCAAGTTGTGACCTTGCTCCTAACATTCCACCCAACAACCTACAACATTTCATCAAGGTACACTCCATGTTTGGAAAGAACAATGTGTCGAAGATGTTGAAAAAACTTGATGTTTCACATCATGAACAAGCTATTGAATCCTTGGTGTAA
Protein sequence	>MS.gene46367.t1 MARAIIRLCPACKHLRRECDSSCDLAPNIPPNNLQHFIKVHSMFGKNNVSKMLKKLDVSHHEQAIESLV