AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46532

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46532.t1 MTR_7g037260 64.596 161 5 1 2 110 197 357 3.03e-61 191
MS.gene46532.t1 MTR_7g037130 62.733 161 8 1 2 110 197 357 1.36e-59 186
MS.gene46532.t1 MTR_7g451170 52.071 169 21 2 2 110 243 411 4.97e-48 158
MS.gene46532.t1 MTR_4g088015 50.296 169 24 2 2 110 273 441 2.47e-46 154
MS.gene46532.t1 MTR_7g035300 46.721 122 45 2 3 110 117 232 3.13e-29 105
MS.gene46532.t1 MTR_3g077650 73.684 57 15 0 2 58 233 289 3.62e-24 95.5
MS.gene46532.t1 MTR_4g105660 71.930 57 16 0 2 58 232 288 5.27e-24 94.7
MS.gene46532.t1 MTR_3g463400 66.071 56 19 0 2 57 185 240 1.81e-23 93.6
MS.gene46532.t1 MTR_5g070020 64.286 56 20 0 2 57 174 229 2.47e-22 89.7
MS.gene46532.t1 MTR_7g450950 78.182 55 11 1 5 58 212 266 5.53e-22 88.6
MS.gene46532.t1 MTR_1g057980 58.974 78 31 1 2 78 132 209 7.30e-22 87.4
MS.gene46532.t1 MTR_7g035350 74.074 54 14 0 2 55 277 330 1.27e-21 88.2
MS.gene46532.t1 MTR_5g049190 66.038 53 18 0 2 54 71 123 4.31e-20 82.0
MS.gene46532.t1 MTR_4g019370 61.224 49 19 0 2 50 90 138 1.02e-16 74.3
MS.gene46532.t1 MTR_3g110028 41.463 82 44 1 2 83 137 214 4.25e-16 72.8
MS.gene46532.t1 MTR_1g026870 51.667 60 29 0 2 61 137 196 6.12e-16 72.4
MS.gene46532.t1 MTR_7g061330 57.143 49 21 0 2 50 165 213 7.39e-15 69.3
MS.gene46532.t1 MTR_7g461410 57.143 49 21 0 2 50 165 213 7.39e-15 69.3
MS.gene46532.t1 MTR_7g061330 57.143 49 21 0 2 50 129 177 9.44e-15 68.9
MS.gene46532.t1 MTR_5g082910 64.286 42 15 0 5 46 74 115 4.55e-14 66.2
MS.gene46532.t1 MTR_5g041570 50.909 55 27 0 2 56 186 240 6.69e-14 66.6
MS.gene46532.t1 MTR_4g073420 54.545 55 22 1 5 59 70 121 1.26e-13 65.1
MS.gene46532.t1 MTR_4g094982 57.447 47 20 0 4 50 59 105 2.18e-13 64.3
MS.gene46532.t1 MTR_2g064160 53.191 47 22 0 4 50 68 114 2.86e-13 64.3
MS.gene46532.t1 MTR_5g010650 60.465 43 17 0 2 44 162 204 4.54e-13 64.3
MS.gene46532.t1 MTR_5g016510 57.447 47 20 0 4 50 59 105 5.12e-13 63.5
MS.gene46532.t1 MTR_3g101290 56.522 46 20 0 5 50 92 137 6.17e-13 62.8
MS.gene46532.t1 MTR_8g095390 52.174 46 22 0 5 50 70 115 7.83e-13 63.2
MS.gene46532.t1 MTR_6g015455 57.447 47 20 0 4 50 59 105 8.22e-13 63.5
MS.gene46532.t1 MTR_4g082040 47.458 59 28 1 2 57 157 215 1.19e-12 62.8
MS.gene46532.t1 MTR_6g074860 52.632 57 24 1 5 58 59 115 1.44e-12 62.0
MS.gene46532.t1 MTR_5g029840 46.939 49 26 0 5 53 70 118 3.12e-12 61.6
MS.gene46532.t1 MTR_2g097910 37.097 62 39 0 4 65 77 138 3.75e-12 61.2
MS.gene46532.t1 MTR_3g097450 51.064 47 23 0 4 50 69 115 3.90e-12 61.2
MS.gene46532.t1 MTR_8g017390 42.373 59 34 0 4 62 70 128 4.46e-12 61.2
MS.gene46532.t1 MTR_8g017440 48.936 47 24 0 4 50 69 115 4.76e-12 61.2
MS.gene46532.t1 MTR_8g017540 48.936 47 24 0 4 50 115 161 4.95e-12 61.2
MS.gene46532.t1 MTR_8g017340 53.191 47 22 0 4 50 70 116 5.28e-12 61.2
MS.gene46532.t1 MTR_8g027345 42.623 61 34 1 6 65 71 131 5.90e-12 60.8
MS.gene46532.t1 MTR_2g099740 48.936 47 24 0 4 50 69 115 8.19e-12 60.5
MS.gene46532.t1 MTR_6g055910 46.939 49 25 1 8 56 6 53 1.23e-11 58.9
MS.gene46532.t1 MTR_3g052450 52.941 51 23 1 2 51 95 145 1.27e-11 60.1
MS.gene46532.t1 MTR_8g017350 51.064 47 23 0 4 50 70 116 1.51e-11 59.7
MS.gene46532.t1 MTR_3g052430 46.154 65 29 2 2 65 96 155 1.70e-11 59.7
MS.gene46532.t1 MTR_7g076740 53.061 49 22 1 5 52 71 119 1.81e-11 59.3
MS.gene46532.t1 MTR_5g079670 48.980 49 25 0 5 53 69 117 1.83e-11 58.5
MS.gene46532.t1 MTR_7g110830 46.154 52 25 1 4 52 69 120 1.91e-11 59.3
MS.gene46532.t1 MTR_3g028740 44.681 47 26 0 4 50 79 125 1.91e-11 59.3
MS.gene46532.t1 MTR_4g063100 44.000 50 28 0 6 55 2 51 2.07e-11 58.2
MS.gene46532.t1 MTR_5g010020 56.098 41 18 0 5 45 70 110 2.12e-11 59.3
MS.gene46532.t1 MTR_4g091490 53.488 43 20 0 8 50 74 116 2.32e-11 59.3
MS.gene46532.t1 MTR_8g098860 53.659 41 19 0 5 45 70 110 2.46e-11 58.9
MS.gene46532.t1 MTR_4g063100 44.000 50 28 0 6 55 37 86 2.85e-11 58.2
MS.gene46532.t1 MTR_6g009430 51.923 52 24 1 3 54 68 118 3.08e-11 58.9
MS.gene46532.t1 MTR_6g055910 46.000 50 26 1 7 56 46 94 3.09e-11 58.5
MS.gene46532.t1 MTR_1g085640 39.216 51 31 0 4 54 69 119 3.27e-11 58.9
MS.gene46532.t1 MTR_5g079220 43.396 53 30 0 2 54 76 128 3.62e-11 58.5
MS.gene46532.t1 MTR_8g042410 50.000 52 25 1 2 52 92 143 3.77e-11 58.5
MS.gene46532.t1 MTR_8g017500 46.809 47 25 0 4 50 69 115 4.49e-11 58.5
MS.gene46532.t1 MTR_8g031360 50.000 46 23 0 5 50 72 117 4.53e-11 58.2
MS.gene46532.t1 MTR_4g063100 44.000 50 28 0 6 55 66 115 4.56e-11 57.8
MS.gene46532.t1 MTR_4g102380 47.917 48 25 0 6 53 71 118 4.73e-11 58.2
MS.gene46532.t1 MTR_2g011660 53.488 43 20 0 8 50 74 116 5.12e-11 58.2
MS.gene46532.t1 MTR_7g017260 44.444 54 30 0 2 55 82 135 5.26e-11 57.8
MS.gene46532.t1 MTR_5g078860 48.980 49 25 0 2 50 62 110 5.64e-11 58.2
MS.gene46532.t1 MTR_4g485530 47.059 51 27 0 4 54 69 119 5.87e-11 57.0
MS.gene46532.t1 MTR_6g055910 46.000 50 26 1 7 56 75 123 5.99e-11 57.8
MS.gene46532.t1 MTR_4g125520 39.344 61 35 1 6 64 71 131 7.69e-11 57.8
MS.gene46532.t1 MTR_5g079290 43.396 53 30 0 2 54 72 124 8.43e-11 57.4
MS.gene46532.t1 MTR_7g117730 55.000 40 18 0 6 45 71 110 8.44e-11 57.4
MS.gene46532.t1 MTR_4g065017 41.379 58 31 1 4 61 74 128 8.58e-11 57.4
MS.gene46532.t1 MTR_2g089620 47.826 46 24 0 5 50 70 115 8.99e-11 57.4
MS.gene46532.t1 MTR_3g011610 48.077 52 26 1 2 52 92 143 9.63e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46532.t1 AT4G18770 56.322 87 37 1 2 88 269 354 2.56e-25 98.6
MS.gene46532.t1 AT3G27785 51.648 91 39 2 2 87 241 331 3.37e-24 95.5
MS.gene46532.t1 AT5G40360 70.175 57 17 0 2 58 210 266 4.09e-22 89.0
MS.gene46532.t1 AT5G58850 64.286 56 20 0 2 57 157 212 1.29e-21 88.2
MS.gene46532.t1 AT5G11050 66.071 56 19 0 2 57 157 212 2.17e-21 87.4
MS.gene46532.t1 AT2G25230 64.151 53 19 0 2 54 78 130 3.16e-20 82.0
MS.gene46532.t1 AT5G40430 61.538 52 20 0 2 53 105 156 1.05e-17 75.9
MS.gene46532.t1 AT5G11510 46.552 58 31 0 2 59 133 190 4.62e-15 70.1
MS.gene46532.t1 AT5G11510 46.552 58 31 0 2 59 133 190 5.33e-15 69.7
MS.gene46532.t1 AT5G11510 46.552 58 31 0 2 59 133 190 5.33e-15 69.7
MS.gene46532.t1 AT5G11510 46.552 58 31 0 2 59 133 190 5.33e-15 69.7
MS.gene46532.t1 AT1G69560 41.975 81 43 1 2 78 175 255 9.92e-15 68.6
MS.gene46532.t1 AT4G32730 55.102 49 22 0 2 50 139 187 1.16e-14 68.9
MS.gene46532.t1 AT4G32730 55.102 49 22 0 2 50 139 187 1.16e-14 68.9
MS.gene46532.t1 AT4G32730 55.102 49 22 0 2 50 139 187 1.16e-14 68.9
MS.gene46532.t1 AT4G32730 55.102 49 22 0 2 50 139 187 1.16e-14 68.9
MS.gene46532.t1 AT1G69560 41.975 81 43 1 2 78 159 239 1.18e-14 68.6
MS.gene46532.t1 AT3G09230 41.111 90 48 1 1 85 106 195 1.25e-14 68.6
MS.gene46532.t1 AT4G32730 55.102 49 22 0 2 50 139 187 1.31e-14 68.6
MS.gene46532.t1 AT5G11510 46.552 58 31 0 2 59 133 190 1.53e-14 68.6
MS.gene46532.t1 AT5G02320 53.061 49 23 0 2 50 179 227 1.78e-14 68.2
MS.gene46532.t1 AT5G02320 53.061 49 23 0 2 50 179 227 1.78e-14 68.2
MS.gene46532.t1 AT3G55730 50.877 57 28 0 2 58 108 164 2.00e-14 68.2
MS.gene46532.t1 AT5G02320 53.061 49 23 0 2 50 119 167 2.28e-14 67.8
MS.gene46532.t1 AT1G57560 32.632 95 51 1 4 85 69 163 2.70e-14 67.0
MS.gene46532.t1 AT3G09370 55.102 49 22 0 2 50 195 243 4.24e-14 67.0
MS.gene46532.t1 AT3G09370 55.102 49 22 0 2 50 187 235 4.27e-14 67.0
MS.gene46532.t1 AT4G00540 56.000 50 22 0 1 50 157 206 4.73e-14 67.0
MS.gene46532.t1 AT3G09370 55.102 49 22 0 2 50 182 230 4.88e-14 67.0
MS.gene46532.t1 AT4G00540 56.000 50 22 0 1 50 155 204 5.99e-14 66.6
MS.gene46532.t1 AT3G12720 55.319 47 21 0 4 50 79 125 1.01e-13 65.9
MS.gene46532.t1 AT4G38620 54.545 55 22 1 5 59 70 121 1.31e-13 65.1
MS.gene46532.t1 AT1G26780 55.102 49 22 0 2 50 150 198 2.87e-13 64.7
MS.gene46532.t1 AT2G32460 55.769 52 22 1 2 52 73 124 2.88e-13 64.7
MS.gene46532.t1 AT1G26780 55.102 49 22 0 2 50 150 198 3.02e-13 64.3
MS.gene46532.t1 AT5G67300 49.180 61 31 0 5 65 61 121 3.63e-13 63.9
MS.gene46532.t1 AT3G11440 50.000 52 25 1 2 52 96 147 4.27e-13 64.3
MS.gene46532.t1 AT3G11440 50.000 52 25 1 2 52 96 147 4.27e-13 64.3
MS.gene46532.t1 AT3G11440 50.000 52 25 1 2 52 96 147 4.27e-13 64.3
MS.gene46532.t1 AT3G11440 50.000 52 25 1 2 52 96 147 5.28e-13 63.9
MS.gene46532.t1 AT2G26950 44.615 65 32 1 2 66 71 131 5.90e-13 63.9
MS.gene46532.t1 AT1G17950 47.541 61 29 1 2 59 57 117 6.83e-13 62.8
MS.gene46532.t1 AT1G22640 54.348 46 21 0 5 50 13 58 7.01e-13 62.0
MS.gene46532.t1 AT2G32460 55.769 52 22 1 2 52 61 112 7.89e-13 63.5
MS.gene46532.t1 AT5G14750 44.068 59 27 1 4 56 12 70 1.57e-12 60.1
MS.gene46532.t1 AT5G14750 44.068 59 27 1 4 56 12 70 1.57e-12 60.1
MS.gene46532.t1 AT3G28470 51.163 43 21 0 3 45 68 110 1.58e-12 62.4
MS.gene46532.t1 AT2G23290 46.875 64 34 0 5 68 68 131 1.85e-12 62.0
MS.gene46532.t1 AT1G22640 54.348 46 21 0 5 50 70 115 2.44e-12 61.6
MS.gene46532.t1 AT2G39880 43.396 53 30 0 2 54 102 154 2.61e-12 62.0
MS.gene46532.t1 AT4G33450 54.000 50 23 0 2 51 71 120 2.91e-12 61.2
MS.gene46532.t1 AT4G37260 51.020 49 24 0 4 52 67 115 3.20e-12 61.6
MS.gene46532.t1 AT4G09460 52.174 46 22 0 5 50 70 115 3.27e-12 60.8
MS.gene46532.t1 AT3G13890 48.936 47 24 0 4 50 78 124 3.61e-12 61.6
MS.gene46532.t1 AT3G53200 49.057 53 26 1 6 58 35 86 3.70e-12 60.5
MS.gene46532.t1 AT5G14750 44.068 59 27 1 4 56 39 97 3.95e-12 59.7
MS.gene46532.t1 AT1G57560 40.000 65 38 1 4 67 69 133 4.01e-12 61.2
MS.gene46532.t1 AT4G34990 49.020 51 26 0 4 54 69 119 4.27e-12 60.8
MS.gene46532.t1 AT5G17800 46.774 62 32 1 2 62 145 206 5.09e-12 60.8
MS.gene46532.t1 AT5G06100 48.077 52 26 1 2 52 87 138 5.36e-12 61.2
MS.gene46532.t1 AT3G13890 48.936 47 24 0 4 50 69 115 5.37e-12 61.2
MS.gene46532.t1 AT3G27920 41.538 65 37 1 2 65 69 133 5.71e-12 60.1
MS.gene46532.t1 AT5G06100 48.077 52 26 1 2 52 87 138 5.79e-12 61.2
MS.gene46532.t1 AT5G06100 48.077 52 26 1 2 52 87 138 5.79e-12 61.2
MS.gene46532.t1 AT5G06100 48.077 52 26 1 2 52 87 138 5.79e-12 61.2
MS.gene46532.t1 AT5G06100 48.077 52 26 1 2 52 87 138 5.79e-12 61.2
MS.gene46532.t1 AT3G53200 49.057 53 26 1 6 58 68 119 7.42e-12 60.1
MS.gene46532.t1 AT4G28110 48.936 47 24 0 8 54 73 119 8.02e-12 60.1
MS.gene46532.t1 AT5G59780 45.455 55 29 1 6 60 2 55 8.95e-12 58.9
MS.gene46532.t1 AT4G26930 43.548 62 33 1 2 63 74 133 9.01e-12 60.5
MS.gene46532.t1 AT2G16720 47.059 51 27 0 4 54 69 119 1.22e-11 59.7
MS.gene46532.t1 AT5G35550 42.308 52 30 0 6 57 76 127 1.33e-11 59.3
MS.gene46532.t1 AT5G14750 52.381 42 20 0 4 45 73 114 1.35e-11 58.9
MS.gene46532.t1 AT2G26960 52.083 48 23 0 2 49 75 122 1.66e-11 59.7
MS.gene46532.t1 AT3G46130 45.455 55 29 1 6 60 2 55 1.99e-11 58.2
MS.gene46532.t1 AT4G05100 52.174 46 22 0 8 53 74 119 2.12e-11 59.3
MS.gene46532.t1 AT1G09540 42.857 56 29 1 4 59 69 121 2.27e-11 59.3
MS.gene46532.t1 AT1G18570 40.909 66 34 1 5 65 71 136 2.53e-11 59.3
MS.gene46532.t1 AT5G59780 45.455 55 29 1 6 60 46 99 2.63e-11 58.2
MS.gene46532.t1 AT5G40330 51.220 41 20 0 5 45 70 110 2.90e-11 58.2
MS.gene46532.t1 AT5G12870 44.000 50 28 0 4 53 75 124 2.98e-11 58.5
MS.gene46532.t1 AT5G35550 42.308 52 30 0 6 57 73 124 3.04e-11 58.5
MS.gene46532.t1 AT3G08500 42.857 49 28 0 2 50 85 133 3.31e-11 58.9
MS.gene46532.t1 AT5G26660 41.176 51 30 0 4 54 38 88 3.41e-11 58.5
MS.gene46532.t1 AT5G59780 45.455 55 29 1 6 60 67 120 3.45e-11 58.2
MS.gene46532.t1 AT3G50060 54.348 46 21 0 5 50 61 106 3.79e-11 58.5
MS.gene46532.t1 AT1G74430 44.000 50 28 0 5 54 70 119 4.12e-11 58.2
MS.gene46532.t1 AT5G16770 50.000 46 23 0 5 50 70 115 4.32e-11 58.5
MS.gene46532.t1 AT5G16770 50.000 46 23 0 5 50 70 115 4.32e-11 58.5
MS.gene46532.t1 AT5G55020 47.917 48 25 0 2 49 81 128 4.34e-11 58.5
MS.gene46532.t1 AT4G12350 43.750 48 27 0 6 53 71 118 4.71e-11 58.2
MS.gene46532.t1 AT3G46130 45.455 55 29 1 6 60 46 99 5.18e-11 57.8
MS.gene46532.t1 AT3G46130 45.455 55 29 1 6 60 36 89 5.19e-11 57.4
MS.gene46532.t1 AT3G46130 45.455 55 29 1 6 60 68 121 5.75e-11 57.8
MS.gene46532.t1 AT5G16770 48.936 47 24 0 4 50 69 115 6.49e-11 57.8
MS.gene46532.t1 AT5G16770 48.936 47 24 0 4 50 69 115 6.49e-11 57.8
MS.gene46532.t1 AT5G26660 41.176 51 30 0 4 54 69 119 7.56e-11 57.8
MS.gene46532.t1 AT4G22680 45.833 48 26 0 6 53 71 118 7.92e-11 57.4

Find 40 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTCTTCTTGGCTTTC+TGG 0.037710 7.1:+59732068 None:intergenic
TCCTGCTTTATAGGAATTTC+AGG 0.183391 7.1:+59731781 None:intergenic
GTTACCCCGATTCTTCTTCT+TGG 0.263788 7.1:+59732060 None:intergenic
TAGCCACCAATTTCATCTTC+TGG 0.310919 7.1:+59731874 None:intergenic
ACTACTCCAGAAGATGAAAT+TGG 0.319338 7.1:-59731880 MS.gene46532:CDS
GGCTTTCTGGCGGCGCTTAG+TGG 0.348988 7.1:+59732081 None:intergenic
GGAAATTGCAAGGAGGTTGC+CGG 0.361526 7.1:-59732140 MS.gene46532:CDS
GTGGTTCTTGACGGAGTTCT+CGG 0.386546 7.1:+59732111 None:intergenic
GATGGAATGGCAAGTGGGTT+TGG 0.392878 7.1:-59731826 MS.gene46532:CDS
CCCTGAAATTCCTATAAAGC+AGG 0.433661 7.1:-59731782 MS.gene46532:CDS
GGCGTTCCAGTGGTTCTTGA+CGG 0.436794 7.1:+59732102 None:intergenic
GAAATTGCAAGGAGGTTGCC+GGG 0.440844 7.1:-59732139 MS.gene46532:CDS
TCATCATCATTGAACATCAT+TGG 0.444693 7.1:+59731847 None:intergenic
AGAAAGCCAAGAAGAAGAAT+CGG 0.447063 7.1:-59732066 MS.gene46532:CDS
AAGATATATAGAAACTCTTA+AGG 0.471628 7.1:-59731742 None:intergenic
CCTGCTTTATAGGAATTTCA+GGG 0.476507 7.1:+59731782 None:intergenic
GAAAGCCAAGAAGAAGAATC+GGG 0.489419 7.1:-59732065 MS.gene46532:CDS
ATCGAAGCTCACAAGATAGT+TGG 0.496519 7.1:-59732175 MS.gene46532:CDS
AATGATTAAAGAAGGAGTCA+TGG 0.536087 7.1:-59732222 MS.gene46532:intron
ACGGAGTTCTCGGTGCGCCC+CGG 0.540891 7.1:+59732121 None:intergenic
GCAAGTGGGTTTGGATCGAA+TGG 0.547112 7.1:-59731817 MS.gene46532:CDS
AAAGCAGGAAAATGACCTCA+TGG 0.557021 7.1:-59731767 MS.gene46532:CDS
GGCGCTTAGTGGCGTTCCAG+TGG 0.575961 7.1:+59732092 None:intergenic
AGAACTCCGTCAAGAACCAC+TGG 0.577545 7.1:-59732108 MS.gene46532:CDS
GTTCAATGATGATGATGGAA+TGG 0.583601 7.1:-59731839 MS.gene46532:CDS
ACAAGATAGTTGGTAACAAG+TGG 0.585557 7.1:-59732165 MS.gene46532:CDS
TGGGTTTGGATCGAATGGAA+TGG 0.596652 7.1:-59731812 MS.gene46532:CDS
ATGATGTTCAATGATGATGA+TGG 0.603439 7.1:-59731844 MS.gene46532:CDS
ACAAGTGGGCGGAAATTGCA+AGG 0.615105 7.1:-59732150 MS.gene46532:CDS
ACTCCAGAAGATGAAATTGG+TGG 0.615237 7.1:-59731877 MS.gene46532:CDS
ATGATGATGGAATGGCAAGT+GGG 0.639684 7.1:-59731831 MS.gene46532:CDS
TTCTATATATCTTCTCCATG+AGG 0.643428 7.1:+59731752 None:intergenic
GATGATGATGGAATGGCAAG+TGG 0.644418 7.1:-59731832 MS.gene46532:CDS
GAAGGAGTCATGGACCGAAG+AGG 0.656279 7.1:-59732212 MS.gene46532:CDS
CAAGATAGTTGGTAACAAGT+GGG 0.659165 7.1:-59732164 MS.gene46532:CDS
AAATTGCAAGGAGGTTGCCG+GGG 0.666053 7.1:-59732138 MS.gene46532:CDS
TCTTCTTCTTGGCTTTCTGG+CGG 0.705181 7.1:+59732071 None:intergenic
GATAGTTGGTAACAAGTGGG+CGG 0.706945 7.1:-59732161 MS.gene46532:CDS
AAAGCCAAGAAGAAGAATCG+GGG 0.727248 7.1:-59732064 MS.gene46532:intron
AGTGGGCGGAAATTGCAAGG+AGG 0.792906 7.1:-59732147 MS.gene46532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATCTAAATATATTATAT+AGG - chr7.1:59732087-59732106 MS.gene46532:CDS 10.0%
!! ATTAAATTAATTCACCAATT+TGG + chr7.1:59732308-59732327 None:intergenic 15.0%
!! CATTCAAAAAAGTTAATAAA+TGG + chr7.1:59732349-59732368 None:intergenic 15.0%
!! TAAAATTAAAATAGTGTACA+AGG - chr7.1:59732251-59732270 MS.gene46532:intron 15.0%
!! GAAAACTATGATATAAATGA+GGG - chr7.1:59731786-59731805 MS.gene46532:CDS 20.0%
!! TGAAAACTATGATATAAATG+AGG - chr7.1:59731785-59731804 MS.gene46532:CDS 20.0%
!!! AAATTTTGTCAATGTTACAA+CGG + chr7.1:59732049-59732068 None:intergenic 20.0%
!!! ACTACTTTTGAAATACATAA+TGG - chr7.1:59732564-59732583 MS.gene46532:intron 20.0%
!!! CTTTTTTGAATGATTAAAGA+AGG - chr7.1:59732357-59732376 MS.gene46532:intron 20.0%
! AAGTACTCAATTTCAATTGT+TGG + chr7.1:59731860-59731879 None:intergenic 25.0%
! AGAATAAGCTATTACCAAAT+TGG - chr7.1:59732291-59732310 MS.gene46532:intron 25.0%
!! ATTTTCACACAAGTAAATGA+TGG - chr7.1:59731758-59731777 MS.gene46532:CDS 25.0%
AAACAAAACTTCAGATTGCT+TGG - chr7.1:59732192-59732211 MS.gene46532:CDS 30.0%
AACTATGATATAAATGAGGG+TGG - chr7.1:59731789-59731808 MS.gene46532:CDS 30.0%
AATGATTAAAGAAGGAGTCA+TGG - chr7.1:59732365-59732384 MS.gene46532:intron 30.0%
ACTCGTGATGTATATAACAT+CGG + chr7.1:59732149-59732168 None:intergenic 30.0%
CTCTCAGAACTTTCATAATT+TGG + chr7.1:59732664-59732683 None:intergenic 30.0%
TCACGAGTATTATACATGAA+TGG - chr7.1:59732161-59732180 MS.gene46532:CDS 30.0%
TCATCATCATTGAACATCAT+TGG + chr7.1:59732743-59732762 None:intergenic 30.0%
TTCTACCAAGAAAGTTGAAA+AGG - chr7.1:59732594-59732613 MS.gene46532:intron 30.0%
TTCTATATATCTTCTCCATG+AGG + chr7.1:59732838-59732857 None:intergenic 30.0%
! ATGATGTTCAATGATGATGA+TGG - chr7.1:59732743-59732762 MS.gene46532:intron 30.0%
! CAATATTTTGTCTTCCTCTT+CGG + chr7.1:59732392-59732411 None:intergenic 30.0%
! TCTAAGAGCATGATGAATAT+CGG - chr7.1:59732631-59732650 MS.gene46532:intron 30.0%
!!! AGAGTGATTTTTTTCTCTGA+AGG - chr7.1:59732680-59732699 MS.gene46532:intron 30.0%
ACTACTCCAGAAGATGAAAT+TGG - chr7.1:59732707-59732726 MS.gene46532:intron 35.0%
AGAAAGCCAAGAAGAAGAAT+CGG - chr7.1:59732521-59732540 MS.gene46532:intron 35.0%
CCTGCTTTATAGGAATTTCA+GGG + chr7.1:59732808-59732827 None:intergenic 35.0%
CTCGTGATGTATATAACATC+GGG + chr7.1:59732148-59732167 None:intergenic 35.0%
TCCTGCTTTATAGGAATTTC+AGG + chr7.1:59732809-59732828 None:intergenic 35.0%
! CATCTCCTTTTCAACTTTCT+TGG + chr7.1:59732602-59732621 None:intergenic 35.0%
! CTAAGAGCATGATGAATATC+GGG - chr7.1:59732632-59732651 MS.gene46532:intron 35.0%
!! ACAAGATAGTTGGTAACAAG+TGG - chr7.1:59732422-59732441 MS.gene46532:intron 35.0%
!! AGGTCATTTTCCTGCTTTAT+AGG + chr7.1:59732818-59732837 None:intergenic 35.0%
!! CAAGATAGTTGGTAACAAGT+GGG - chr7.1:59732423-59732442 MS.gene46532:intron 35.0%
!! GTTCAATGATGATGATGGAA+TGG - chr7.1:59732748-59732767 MS.gene46532:intron 35.0%
AAAGCAGGAAAATGACCTCA+TGG - chr7.1:59732820-59732839 MS.gene46532:intron 40.0%
AAAGCCAAGAAGAAGAATCG+GGG - chr7.1:59732523-59732542 MS.gene46532:intron 40.0%
AATCGGGGTAACAGTTCAAA+GGG - chr7.1:59732538-59732557 MS.gene46532:intron 40.0%
ACTCCAGAAGATGAAATTGG+TGG - chr7.1:59732710-59732729 MS.gene46532:intron 40.0%
ATCGAAGCTCACAAGATAGT+TGG - chr7.1:59732412-59732431 MS.gene46532:intron 40.0%
CCCTGAAATTCCTATAAAGC+AGG - chr7.1:59732805-59732824 MS.gene46532:intron 40.0%
GAAAGCCAAGAAGAAGAATC+GGG - chr7.1:59732522-59732541 MS.gene46532:intron 40.0%
TAAATGAGGGTGGATCAAGT+TGG - chr7.1:59731799-59731818 MS.gene46532:CDS 40.0%
TAGCCACCAATTTCATCTTC+TGG + chr7.1:59732716-59732735 None:intergenic 40.0%
! GATTCTTCTTCTTGGCTTTC+TGG + chr7.1:59732522-59732541 None:intergenic 40.0%
!! ATGATGATGGAATGGCAAGT+GGG - chr7.1:59732756-59732775 MS.gene46532:intron 40.0%
GAATCGGGGTAACAGTTCAA+AGG - chr7.1:59732537-59732556 MS.gene46532:intron 45.0%
GTTACCCCGATTCTTCTTCT+TGG + chr7.1:59732530-59732549 None:intergenic 45.0%
TGGGTTTGGATCGAATGGAA+TGG - chr7.1:59732775-59732794 MS.gene46532:intron 45.0%
! TCTTCTTCTTGGCTTTCTGG+CGG + chr7.1:59732519-59732538 None:intergenic 45.0%
!! GATAGTTGGTAACAAGTGGG+CGG - chr7.1:59732426-59732445 MS.gene46532:intron 45.0%
!! GATGATGATGGAATGGCAAG+TGG - chr7.1:59732755-59732774 MS.gene46532:intron 45.0%
AAATTGCAAGGAGGTTGCCG+GGG - chr7.1:59732449-59732468 MS.gene46532:intron 50.0%
ACAAGTGGGCGGAAATTGCA+AGG - chr7.1:59732437-59732456 MS.gene46532:intron 50.0%
AGAACTCCGTCAAGAACCAC+TGG - chr7.1:59732479-59732498 MS.gene46532:intron 50.0%
GAAATTGCAAGGAGGTTGCC+GGG - chr7.1:59732448-59732467 MS.gene46532:intron 50.0%
GGAAATTGCAAGGAGGTTGC+CGG - chr7.1:59732447-59732466 MS.gene46532:intron 50.0%
GTGGTTCTTGACGGAGTTCT+CGG + chr7.1:59732479-59732498 None:intergenic 50.0%
! GATGGAATGGCAAGTGGGTT+TGG - chr7.1:59732761-59732780 MS.gene46532:intron 50.0%
! GCAAGTGGGTTTGGATCGAA+TGG - chr7.1:59732770-59732789 MS.gene46532:intron 50.0%
AGTGGGCGGAAATTGCAAGG+AGG - chr7.1:59732440-59732459 MS.gene46532:intron 55.0%
GAAGGAGTCATGGACCGAAG+AGG - chr7.1:59732375-59732394 MS.gene46532:intron 55.0%
GGCGTTCCAGTGGTTCTTGA+CGG + chr7.1:59732488-59732507 None:intergenic 55.0%
GGCGCTTAGTGGCGTTCCAG+TGG + chr7.1:59732498-59732517 None:intergenic 65.0%
! GGCTTTCTGGCGGCGCTTAG+TGG + chr7.1:59732509-59732528 None:intergenic 65.0%
! ACGGAGTTCTCGGTGCGCCC+CGG + chr7.1:59732469-59732488 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 59731744 59732865 59731744 ID=MS.gene46532
chr7.1 mRNA 59731744 59732865 59731744 ID=MS.gene46532.t1;Parent=MS.gene46532
chr7.1 exon 59732863 59732865 59732863 ID=MS.gene46532.t1.exon1;Parent=MS.gene46532.t1
chr7.1 CDS 59732863 59732865 59732863 ID=cds.MS.gene46532.t1;Parent=MS.gene46532.t1
chr7.1 exon 59732065 59732233 59732065 ID=MS.gene46532.t1.exon2;Parent=MS.gene46532.t1
chr7.1 CDS 59732065 59732233 59732065 ID=cds.MS.gene46532.t1;Parent=MS.gene46532.t1
chr7.1 exon 59731744 59731907 59731744 ID=MS.gene46532.t1.exon3;Parent=MS.gene46532.t1
chr7.1 CDS 59731744 59731907 59731744 ID=cds.MS.gene46532.t1;Parent=MS.gene46532.t1
Gene Sequence

>MS.gene46532

ATGGTGTGTCATTAATTTTCACACAAGTAAATGATGGATGTTGAAAACTATGATATAAATGAGGGTGGATCAAGTTGGTTTATGCAAGATTTTCATAAAATTATGTTGTCAATCCAACAATTGAAATTGAGTACTTGTACATAGCTATACAAGCATATCGTTGATGCATAAATTATATTTTAATAATTATTTTATACATTTAATTTAAGTAAATTGATAAAATTAAACATTTTTTAAATTGTAATATAAGTTATTAATTTTACGTTTGCATATGTAAAATACAAAATTTGCAGATTTATTAACCGTTGTAACATTGACAAAATTTTATTTTAGTCTTGCATATTCTATCTAAATATATTATATAGGTGTTTGATTTCGTATCATGTTTACAACTCTCACGTCCCGATGTTATATACATCACGAGTATTATACATGAATGGATGCAAGAAAACAAAACTTCAGATTGCTTGGAAACAAACAAATGCAATGTCTAGATAGATATATAATTAAAATTAAAATAGTGTACAAGGATGCAAAAATTTACTTGAGAATAAGCTATTACCAAATTGGTGAATTAATTTAATCATATTTATATAACTAATCCATTTATTAACTTTTTTGAATGATTAAAGAAGGAGTCATGGACCGAAGAGGAAGACAAAATATTGATCGAAGCTCACAAGATAGTTGGTAACAAGTGGGCGGAAATTGCAAGGAGGTTGCCGGGGCGCACCGAGAACTCCGTCAAGAACCACTGGAACGCCACTAAGCGCCGCCAGAAAGCCAAGAAGAAGAATCGGGGTAACAGTTCAAAGGGAACACTACTTTTGAAATACATAATGGAAGTCACTTCTACCAAGAAAGTTGAAAAGGAGATGATGAATAATTCTAAGAGCATGATGAATATCGGGAATCAGCCAAATTATGAAAGTTCTGAGAGTGATTTTTTTCTCTGAAGGTTTGACTACTCCAGAAGATGAAATTGGTGGCTATGTGCCAATGATGTTCAATGATGATGATGGAATGGCAAGTGGGTTTGGATCGAATGGAATGGAGTTTTTCCCTGAAATTCCTATAAAGCAGGAAAATGACCTCATGGAGAAGATATATAGAAACTCTTAA
Protein sequence

>MS.gene46532.t1

MKESWTEEEDKILIEAHKIVGNKWAEIARRLPGRTENSVKNHWNATKRRQKAKKKNRGLTTPEDEIGGYVPMMFNDDDGMASGFGSNGMEFFPEIPIKQENDLMEKIYRNS