Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46796.t1 | XP_003599126.2 | 99.4 | 345 | 2 | 0 | 1 | 345 | 1 | 345 | 1.10E-188 | 669.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46796.t1 | E0X9N4 | 66.4 | 345 | 110 | 2 | 1 | 345 | 1 | 339 | 1.6e-119 | 430.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46796.t1 | G7IWL8 | 99.4 | 345 | 2 | 0 | 1 | 345 | 1 | 345 | 8.1e-189 | 669.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene46796.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46796.t1 | MTR_3g028190 | 99.420 | 345 | 2 | 0 | 1 | 345 | 1 | 345 | 0.0 | 715 |
| MS.gene46796.t1 | MTR_3g028170 | 52.599 | 327 | 130 | 7 | 1 | 322 | 1 | 307 | 4.00e-101 | 306 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46796.t1 | AT3G08505 | 63.188 | 345 | 93 | 3 | 1 | 345 | 1 | 311 | 6.53e-160 | 450 |
| MS.gene46796.t1 | AT3G08505 | 63.188 | 345 | 93 | 3 | 1 | 345 | 1 | 311 | 6.53e-160 | 450 |
| MS.gene46796.t1 | AT3G08505 | 63.188 | 345 | 93 | 3 | 1 | 345 | 1 | 311 | 6.53e-160 | 450 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATGTCTTTGGCAATTA+TGG | 0.200868 | 3.3:-20718153 | None:intergenic |
| TTACTTGAGCTTTGCCTTGT+AGG | 0.248432 | 3.3:-20712478 | None:intergenic |
| CAGAAAGGAGTTTGTGCTTA+TGG | 0.262469 | 3.3:+20711733 | MS.gene46796:CDS |
| GACAATGCTTGCATCCTTTC+CGG | 0.265577 | 3.3:+20712121 | MS.gene46796:CDS |
| GCGTATCGTGTATTAGAAAT+TGG | 0.271742 | 3.3:+20712316 | MS.gene46796:CDS |
| TTGCGCAGATTCTTGATTCC+AGG | 0.326851 | 3.3:-20711950 | None:intergenic |
| AGGTAGCTTTGCGTCATCTT+GGG | 0.329256 | 3.3:+20718114 | MS.gene46796:CDS |
| TTGTCGTTCCAAGTGTTATT+TGG | 0.331364 | 3.3:+20712418 | MS.gene46796:CDS |
| CGTAGTAGTAACCCAACATT+AGG | 0.336048 | 3.3:+20712339 | MS.gene46796:CDS |
| GAGGTAGCTTTGCGTCATCT+TGG | 0.340448 | 3.3:+20718113 | MS.gene46796:CDS |
| GTTGTATCACCAACATTATT+CGG | 0.348521 | 3.3:-20711606 | None:intergenic |
| ATTTGTTCATTTGCTGCTGC+TGG | 0.363377 | 3.3:+20712036 | MS.gene46796:CDS |
| CTTTGTAAGTTCTTTGCTCA+TGG | 0.382149 | 3.3:+20711531 | MS.gene46796:CDS |
| ATATGTTCTTCTCTTTCTTC+AGG | 0.386226 | 3.3:-20712144 | None:intergenic |
| TTGATTCCAGGCTTGTTCAC+TGG | 0.388041 | 3.3:-20711938 | None:intergenic |
| GGATGTTAATAGCACATTGC+GGG | 0.414993 | 3.3:+20712365 | MS.gene46796:CDS |
| AGAGAGAAACCTGATGTCTT+TGG | 0.415002 | 3.3:-20718162 | None:intergenic |
| TGATTCCAGGCTTGTTCACT+GGG | 0.416070 | 3.3:-20711937 | None:intergenic |
| GGTGAAGGAAACTGCCCTTT+CGG | 0.424070 | 3.3:+20713124 | MS.gene46796:CDS |
| CGTTCGGTTATTGAAGAAGA+TGG | 0.433534 | 3.3:-20711808 | None:intergenic |
| GTTTCAGAATCGGCTGTACT+TGG | 0.435930 | 3.3:+20711841 | MS.gene46796:CDS |
| ACGGCAGCTGAGCGTAAGTT+TGG | 0.437819 | 3.3:+20712264 | MS.gene46796:CDS |
| GTGCTTATGGTAGTCGCTGC+AGG | 0.448946 | 3.3:+20711746 | MS.gene46796:CDS |
| CTGCGCAACTTGACTTCCTA+AGG | 0.449537 | 3.3:+20711965 | MS.gene46796:CDS |
| CCAGCCCCTTGTTTCAGAAT+CGG | 0.450994 | 3.3:+20711831 | MS.gene46796:CDS |
| CTCATGGAGCGTGTCTGAAA+GGG | 0.457791 | 3.3:+20711547 | MS.gene46796:CDS |
| TGAAACAAGGGGCTGGCGTT+CGG | 0.460548 | 3.3:-20711824 | None:intergenic |
| TACTTCCCAGTGAACAAGCC+TGG | 0.463921 | 3.3:+20711932 | MS.gene46796:CDS |
| TAACATCCATCCCTAATGTT+GGG | 0.466091 | 3.3:-20712350 | None:intergenic |
| CGTCATCTTGGGGCTGCAGA+TGG | 0.471622 | 3.3:+20718125 | MS.gene46796:CDS |
| GGAAGATGATGTTGTTCAAT+CGG | 0.474976 | 3.3:+20711987 | MS.gene46796:CDS |
| CCATAATTGCCAAAGACATC+AGG | 0.475198 | 3.3:+20718153 | MS.gene46796:CDS |
| GAACAACATCATCTTCCCTT+AGG | 0.486955 | 3.3:-20711981 | None:intergenic |
| TTAACATCCATCCCTAATGT+TGG | 0.488690 | 3.3:-20712351 | None:intergenic |
| TAAGATAGCTTGCGACAGAT+AGG | 0.489220 | 3.3:-20712393 | None:intergenic |
| CGGTACTAGCTGCTTCTACA+AGG | 0.489509 | 3.3:+20713144 | MS.gene46796:CDS |
| GCAGCAGCAAATGAACAAAT+CGG | 0.492646 | 3.3:-20712033 | None:intergenic |
| GCTCATGGAGCGTGTCTGAA+AGG | 0.521682 | 3.3:+20711546 | MS.gene46796:CDS |
| TCTCTTTCTTCAGGCCGGAA+AGG | 0.522386 | 3.3:-20712135 | None:intergenic |
| CAACACCATTCGATGTAACT+CGG | 0.526387 | 3.3:-20711870 | None:intergenic |
| GTTCTTCTCTTTCTTCAGGC+CGG | 0.528004 | 3.3:-20712140 | None:intergenic |
| GAACAAATCGGAAGCTCAGA+AGG | 0.538088 | 3.3:-20712021 | None:intergenic |
| CCGATTCTGAAACAAGGGGC+TGG | 0.540796 | 3.3:-20711831 | None:intergenic |
| TACAGCCGATTCTGAAACAA+GGG | 0.542302 | 3.3:-20711836 | None:intergenic |
| GAACAATGTCCTCATGTACA+TGG | 0.543220 | 3.3:+20712075 | MS.gene46796:CDS |
| AGAAGCAGCTAGTACCGAAA+GGG | 0.547432 | 3.3:-20713138 | None:intergenic |
| GTAGTAGTAACCCAACATTA+GGG | 0.548465 | 3.3:+20712340 | MS.gene46796:CDS |
| GCGTGTTCTTTCAAAGCCTA+CGG | 0.552472 | 3.3:+20712245 | MS.gene46796:CDS |
| GGTAGCTTTGCGTCATCTTG+GGG | 0.557694 | 3.3:+20718115 | MS.gene46796:CDS |
| TGTTCACTGGGAAGTACAAA+GGG | 0.566097 | 3.3:-20711925 | None:intergenic |
| TTGTTCACTGGGAAGTACAA+AGG | 0.584820 | 3.3:-20711926 | None:intergenic |
| TATCACCAACATTATTCGGA+GGG | 0.587301 | 3.3:-20711602 | None:intergenic |
| ATATCGTGATGGCCGTCTTG+AGG | 0.591471 | 3.3:+20718091 | MS.gene46796:CDS |
| CAAGCATTGTCTTCCACATG+AGG | 0.592492 | 3.3:-20712109 | None:intergenic |
| ATCACCAACATTATTCGGAG+GGG | 0.593920 | 3.3:-20711601 | None:intergenic |
| TAGTAACCCAACATTAGGGA+TGG | 0.597655 | 3.3:+20712344 | MS.gene46796:CDS |
| AATACCCGAGTTACATCGAA+TGG | 0.599856 | 3.3:+20711865 | MS.gene46796:CDS |
| TATTGAAGAAGATGGAGTAG+AGG | 0.608961 | 3.3:-20711800 | None:intergenic |
| GTAGCATACCAAATAACACT+TGG | 0.613633 | 3.3:-20712426 | None:intergenic |
| AAGCATTGTCTTCCACATGA+GGG | 0.614385 | 3.3:-20712108 | None:intergenic |
| TCGTGATGGCCGTCTTGAGG+AGG | 0.617371 | 3.3:+20718094 | MS.gene46796:CDS |
| AACACCATTCGATGTAACTC+GGG | 0.620122 | 3.3:-20711869 | None:intergenic |
| TAGAAGCAGCTAGTACCGAA+AGG | 0.624207 | 3.3:-20713139 | None:intergenic |
| TGCGCAACTTGACTTCCTAA+GGG | 0.626387 | 3.3:+20711966 | MS.gene46796:CDS |
| GGAGATTTATGCCCCTCATG+TGG | 0.626843 | 3.3:+20712096 | MS.gene46796:CDS |
| GTATCACCAACATTATTCGG+AGG | 0.630350 | 3.3:-20711603 | None:intergenic |
| AGCATTGTCTTCCACATGAG+GGG | 0.631001 | 3.3:-20712107 | None:intergenic |
| GGAAATCATTGATACCTACA+AGG | 0.631452 | 3.3:+20712464 | MS.gene46796:CDS |
| ATAATTTCGCCATGTCAAAG+AGG | 0.631521 | 3.3:+20710510 | None:intergenic |
| AACTTACGCTCAGCTGCCGT+AGG | 0.632534 | 3.3:-20712261 | None:intergenic |
| TGAGGACATTGTTCCCCTCG+TGG | 0.637218 | 3.3:-20712066 | None:intergenic |
| GTACAGCCGATTCTGAAACA+AGG | 0.638170 | 3.3:-20711837 | None:intergenic |
| CTGCTGGAAACTGTCCACGA+GGG | 0.638932 | 3.3:+20712052 | MS.gene46796:CDS |
| TGCTGGAAACTGTCCACGAG+GGG | 0.639094 | 3.3:+20712053 | MS.gene46796:CDS |
| CATAAATCTCCATGTACATG+AGG | 0.639832 | 3.3:-20712084 | None:intergenic |
| CTAATACACGATACGCAGAA+CGG | 0.640856 | 3.3:-20712309 | None:intergenic |
| TCATGGAGCGTGTCTGAAAG+GGG | 0.647946 | 3.3:+20711548 | MS.gene46796:CDS |
| TCTATCTTACCTCTTTGACA+TGG | 0.648394 | 3.3:-20710519 | None:intergenic |
| TTGCAGCATGCATATCGTGA+TGG | 0.670081 | 3.3:+20718080 | MS.gene46796:intron |
| AAATAAGCAAAAGCATCTCG+AGG | 0.674626 | 3.3:+20712179 | MS.gene46796:CDS |
| GCTGCTGGAAACTGTCCACG+AGG | 0.694625 | 3.3:+20712051 | MS.gene46796:CDS |
| TGGATGTTAATAGCACATTG+CGG | 0.695653 | 3.3:+20712364 | MS.gene46796:CDS |
| GCTTTGAAAGAACACGCTCG+AGG | 0.700611 | 3.3:-20712239 | None:intergenic |
| ACAGCCGATTCTGAAACAAG+GGG | 0.720447 | 3.3:-20711835 | None:intergenic |
| GCAAAGCTACCTCCTCAAGA+CGG | 0.724386 | 3.3:-20718103 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 20710521 | 20718174 | 20710521 | ID=MS.gene46796 |
| chr3.3 | mRNA | 20710521 | 20718174 | 20710521 | ID=MS.gene46796.t1;Parent=MS.gene46796 |
| chr3.3 | exon | 20710521 | 20710531 | 20710521 | ID=MS.gene46796.t1.exon1;Parent=MS.gene46796.t1 |
| chr3.3 | CDS | 20710521 | 20710531 | 20710521 | ID=cds.MS.gene46796.t1;Parent=MS.gene46796.t1 |
| chr3.3 | exon | 20711527 | 20711614 | 20711527 | ID=MS.gene46796.t1.exon2;Parent=MS.gene46796.t1 |
| chr3.3 | CDS | 20711527 | 20711614 | 20711527 | ID=cds.MS.gene46796.t1;Parent=MS.gene46796.t1 |
| chr3.3 | exon | 20711718 | 20712496 | 20711718 | ID=MS.gene46796.t1.exon3;Parent=MS.gene46796.t1 |
| chr3.3 | CDS | 20711718 | 20712496 | 20711718 | ID=cds.MS.gene46796.t1;Parent=MS.gene46796.t1 |
| chr3.3 | exon | 20713096 | 20713165 | 20713096 | ID=MS.gene46796.t1.exon4;Parent=MS.gene46796.t1 |
| chr3.3 | CDS | 20713096 | 20713165 | 20713096 | ID=cds.MS.gene46796.t1;Parent=MS.gene46796.t1 |
| chr3.3 | exon | 20718086 | 20718174 | 20718086 | ID=MS.gene46796.t1.exon5;Parent=MS.gene46796.t1 |
| chr3.3 | CDS | 20718086 | 20718174 | 20718086 | ID=cds.MS.gene46796.t1;Parent=MS.gene46796.t1 |
| Gene Sequence |
| Protein sequence |
