AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46828.t1 MTR_3g028520 94.647 411 17 1 1 411 1 406 0.0 804
MS.gene46828.t1 MTR_3g027650 94.647 411 17 1 1 411 1 406 0.0 804
MS.gene46828.t1 MTR_3g027650 90.954 409 28 3 1 408 1 401 0.0 752
MS.gene46828.t1 MTR_3g027650 94.247 365 16 1 1 365 1 360 0.0 703
MS.gene46828.t1 MTR_8g036235 51.111 405 153 10 10 401 18 390 1.22e-107 323
MS.gene46828.t1 MTR_8g036235 49.322 369 142 10 10 365 18 354 1.29e-86 269
MS.gene46828.t1 MTR_1g052470 37.108 415 187 17 14 399 1 370 1.72e-53 182
MS.gene46828.t1 MTR_2g098310 36.009 436 203 19 20 405 1 410 2.45e-52 180
MS.gene46828.t1 MTR_4g065870 33.565 432 209 16 30 406 25 433 4.16e-47 167
MS.gene46828.t1 MTR_2g098310 35.782 422 195 19 20 391 1 396 1.06e-46 165
MS.gene46828.t1 MTR_2g098310 33.333 471 203 20 20 405 1 445 6.80e-45 161
MS.gene46828.t1 MTR_4g065870 33.333 414 199 15 30 388 25 416 9.80e-42 152
MS.gene46828.t1 MTR_7g092540 37.849 251 112 6 166 399 92 315 3.25e-41 148
MS.gene46828.t1 MTR_7g092540 38.430 242 105 6 166 390 92 306 1.43e-38 141
MS.gene46828.t1 MTR_4g033295 68.235 85 27 0 319 403 110 194 3.94e-34 126
MS.gene46828.t1 MTR_2g098310 33.501 397 186 19 20 365 1 370 4.60e-31 123
MS.gene46828.t1 MTR_1g116790 60.577 104 40 1 297 400 236 338 4.84e-31 122
MS.gene46828.t1 MTR_4g033295 73.611 72 19 0 319 390 110 181 4.95e-31 117
MS.gene46828.t1 MTR_4g065870 30.435 391 195 15 30 365 25 393 2.51e-27 112
MS.gene46828.t1 MTR_1g052470 33.241 361 165 17 14 344 1 315 3.97e-26 107
MS.gene46828.t1 MTR_2g098310 31.915 376 178 19 20 344 1 349 5.85e-21 94.0
MS.gene46828.t1 MTR_4g033295 82.979 47 8 0 319 365 110 156 3.53e-20 87.8
MS.gene46828.t1 MTR_6g052280 52.381 84 32 2 290 365 9 92 1.79e-17 78.6
MS.gene46828.t1 MTR_4g131160 54.545 66 29 1 326 391 195 259 5.58e-15 76.6
MS.gene46828.t1 MTR_4g131160 54.545 66 29 1 326 391 198 262 5.98e-15 76.6
MS.gene46828.t1 MTR_3g498825 53.846 65 30 0 327 391 147 211 4.83e-14 73.2
MS.gene46828.t1 MTR_1g080890 47.727 88 38 3 304 391 177 256 2.71e-13 71.2
MS.gene46828.t1 MTR_7g053410 50.769 65 32 0 327 391 317 381 4.64e-13 71.2
MS.gene46828.t1 MTR_8g012290 50.769 65 32 0 327 391 291 355 4.88e-13 70.9
MS.gene46828.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 4.94e-13 70.9
MS.gene46828.t1 MTR_8g012290 50.769 65 32 0 327 391 291 355 5.03e-13 70.9
MS.gene46828.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 5.22e-13 70.5
MS.gene46828.t1 MTR_7g053410 50.769 65 32 0 327 391 317 381 5.28e-13 70.9
MS.gene46828.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 5.70e-13 70.5
MS.gene46828.t1 MTR_1g017350 47.761 67 35 0 325 391 258 324 6.02e-13 70.5
MS.gene46828.t1 MTR_8g065740 50.769 65 32 0 327 391 305 369 7.15e-13 70.5
MS.gene46828.t1 MTR_3g116770 50.769 65 32 0 327 391 191 255 7.48e-13 70.1
MS.gene46828.t1 MTR_3g116770 50.769 65 32 0 327 391 191 255 7.51e-13 70.1
MS.gene46828.t1 MTR_5g048860 43.011 93 48 1 304 391 337 429 2.64e-12 68.9
MS.gene46828.t1 MTR_4g070320 46.053 76 39 1 318 391 132 207 3.63e-12 67.4
MS.gene46828.t1 MTR_8g099880 49.231 65 33 0 327 391 145 209 4.60e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46828.t1 AT2G42280 44.534 247 112 8 166 405 129 357 1.26e-46 164
MS.gene46828.t1 AT1G51140 31.726 394 232 12 19 401 11 378 6.73e-44 157
MS.gene46828.t1 AT2G42280 43.723 231 105 8 166 389 129 341 1.04e-39 145
MS.gene46828.t1 AT1G05805 34.367 387 193 14 32 404 21 360 1.66e-38 142
MS.gene46828.t1 AT2G43140 50.336 149 59 4 259 405 77 212 1.70e-38 138
MS.gene46828.t1 AT2G43140 50.676 148 60 4 259 405 174 309 1.34e-37 139
MS.gene46828.t1 AT1G35460 67.857 84 27 0 322 405 176 259 1.41e-33 126
MS.gene46828.t1 AT4G09180 49.286 140 59 3 268 405 133 262 4.96e-32 122
MS.gene46828.t1 AT1G35460 72.000 50 14 0 319 368 173 222 4.75e-17 80.1
MS.gene46828.t1 AT2G42280 37.634 186 91 8 166 344 129 296 1.99e-16 79.7
MS.gene46828.t1 AT2G24260 56.061 66 28 1 326 391 34 98 1.81e-15 76.3
MS.gene46828.t1 AT5G58010 48.235 85 33 3 317 391 79 162 5.82e-15 75.5
MS.gene46828.t1 AT2G24260 56.061 66 28 1 326 391 137 201 5.83e-15 76.3
MS.gene46828.t1 AT4G30980 54.545 66 29 1 326 391 129 193 1.23e-14 74.7
MS.gene46828.t1 AT4G30980 54.545 66 29 1 326 391 177 241 1.61e-14 74.7
MS.gene46828.t1 AT4G34530 52.308 65 31 0 327 391 172 236 1.06e-13 72.0
MS.gene46828.t1 AT5G50915 50.769 65 32 0 327 391 136 200 2.94e-13 70.1
MS.gene46828.t1 AT5G50915 50.769 65 32 0 327 391 136 200 2.94e-13 70.1
MS.gene46828.t1 AT5G50915 50.769 65 32 0 327 391 136 200 3.61e-13 69.7
MS.gene46828.t1 AT5G50915 50.769 65 32 0 327 391 136 200 3.61e-13 69.7
MS.gene46828.t1 AT1G25330 47.692 65 34 0 327 391 104 168 5.44e-13 68.6
MS.gene46828.t1 AT1G68920 50.769 65 32 0 327 391 302 366 7.46e-13 70.5
MS.gene46828.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.48e-13 70.5
MS.gene46828.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.48e-13 70.5
MS.gene46828.t1 AT1G68920 50.769 65 32 0 327 391 303 367 7.48e-13 70.5
MS.gene46828.t1 AT3G07340 50.769 65 32 0 327 391 258 322 1.33e-12 69.7
MS.gene46828.t1 AT3G07340 50.769 65 32 0 327 391 258 322 1.35e-12 69.3
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 201 265 1.58e-12 68.9
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.62e-12 68.9
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.62e-12 68.9
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.62e-12 68.9
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 223 287 1.62e-12 68.9
MS.gene46828.t1 AT1G26260 50.769 65 32 0 327 391 172 236 1.70e-12 68.6
MS.gene46828.t1 AT1G10120 41.379 87 43 1 305 391 192 270 2.43e-12 68.2
MS.gene46828.t1 AT1G10120 41.379 87 43 1 305 391 192 270 2.43e-12 68.2
MS.gene46828.t1 AT5G48560 49.231 65 33 0 327 391 301 365 3.82e-12 68.2
MS.gene46828.t1 AT5G48560 49.231 65 33 0 327 391 301 365 4.17e-12 68.2
MS.gene46828.t1 AT4G36540 41.463 82 48 0 310 391 124 205 9.16e-12 65.9
MS.gene46828.t1 AT4G36540 41.463 82 48 0 310 391 124 205 9.40e-12 65.9
MS.gene46828.t1 AT1G73830 47.692 65 34 0 327 391 147 211 1.20e-11 65.1
MS.gene46828.t1 AT1G73830 47.692 65 34 0 327 391 147 211 1.22e-11 65.1
MS.gene46828.t1 AT2G18300 46.154 65 35 0 327 391 185 249 6.62e-11 63.5
MS.gene46828.t1 AT2G18300 46.154 65 35 0 327 391 185 249 6.88e-11 63.5
MS.gene46828.t1 AT2G18300 46.154 65 35 0 327 391 185 249 7.82e-11 63.5

Find 56 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAATACCTTATCTGATTT+TGG 0.030579 3.3:+20139448 None:intergenic
CTAGCTTCACCAATGAATTT+TGG 0.135326 3.3:-20139875 MS.gene46828:CDS
CTTGATTCTGTCACTAATTC+TGG 0.137372 3.3:+20139489 None:intergenic
ACCCTCTCTGCTATGCTTCT+TGG 0.144238 3.3:+20139621 None:intergenic
GACTCTCAAGAAGCAGATTT+TGG 0.193647 3.3:-20139780 MS.gene46828:CDS
CATTTACTAGGTTTGTATTT+TGG 0.237692 3.3:+20139925 None:intergenic
GTACCTGAACTTGTTCTTGT+AGG 0.262088 3.3:+20139062 None:intergenic
TACTTGAAGAATCCATTTCT+TGG 0.275479 3.3:+20141409 None:intergenic
CAGAATGGTAATTATTCTTA+TGG 0.286535 3.3:-20141096 MS.gene46828:CDS
GATTCCAATCAATCTAAAAT+TGG 0.297971 3.3:-20140982 MS.gene46828:CDS
ATGCCACAAATAGCTGAAAA+TGG 0.316152 3.3:-20139975 MS.gene46828:CDS
CATTATCAACAACAAAATTC+TGG 0.321728 3.3:-20141369 MS.gene46828:CDS
TTTGAGCGTTGTTGTCGATT+AGG 0.323950 3.3:+20141301 None:intergenic
CACGGCAGATATGTTGGATT+TGG 0.326350 3.3:-20139104 MS.gene46828:CDS
ACAAACCTAGTAAATGAAAA+TGG 0.347655 3.3:-20139918 MS.gene46828:CDS
GGAATTGCTCCAATCTTATT+AGG 0.352113 3.3:-20140954 MS.gene46828:CDS
GAAGCTAGGTATGTGACTTT+TGG 0.365896 3.3:+20139890 None:intergenic
GCAGATACTCACAGATTGTA+AGG 0.393985 3.3:-20138952 MS.gene46828:intron
TGTAAAATTCGAGCGAAAAG+AGG 0.401428 3.3:-20139657 MS.gene46828:CDS
TCCATTTCTGAACTTGTTGA+TGG 0.404102 3.3:+20141234 None:intergenic
TCCATCAACAAGTTCAGAAA+TGG 0.409930 3.3:-20141235 MS.gene46828:CDS
AAACCTGTGAAGCAAGAAAT+AGG 0.413405 3.3:-20141135 MS.gene46828:CDS
AGAATTGTCCCAAAATTCAT+TGG 0.420165 3.3:+20139866 None:intergenic
TCACCTATTTCTTGCTTCAC+AGG 0.431403 3.3:+20141132 None:intergenic
TTCTACTAAGATGACATCTA+TGG 0.437415 3.3:-20139712 MS.gene46828:CDS
GCTAAGAAGAAACCAAGAAA+TGG 0.440153 3.3:-20141421 MS.gene46828:CDS
ATCAATCTAAAATTGGTGTT+AGG 0.440240 3.3:-20140975 MS.gene46828:CDS
CAGAACCATTTGAAAAGCCT+TGG 0.447461 3.3:+20141055 None:intergenic
AAAGCCAATGGCATCTCAAA+TGG 0.469024 3.3:-20140041 MS.gene46828:CDS
GTACACTTAACTTCTCATCT+AGG 0.475567 3.3:-20140019 MS.gene46828:CDS
CGAAGAATCTCTTACAAATG+AGG 0.481362 3.3:-20141265 MS.gene46828:CDS
TCCAAGAAGCATAGCAGAGA+GGG 0.488800 3.3:-20139622 MS.gene46828:intron
AACAAGCACGGCAGATATGT+TGG 0.496382 3.3:-20139110 MS.gene46828:CDS
GGCCATCTACTAGCTTGAAA+AGG 0.500559 3.3:-20139998 MS.gene46828:CDS
AGACCTACAAGAACAAGTTC+AGG 0.503261 3.3:-20139065 MS.gene46828:intron
TGATATCATCTAACAATGGA+TGG 0.526562 3.3:-20141194 MS.gene46828:CDS
TGTACCATTTGAGATGCCAT+TGG 0.531059 3.3:+20140037 None:intergenic
GTAAAATTCGAGCGAAAAGA+GGG 0.539222 3.3:-20139656 MS.gene46828:CDS
AATGGTAATTATTCTTATGG+TGG 0.553621 3.3:-20141093 MS.gene46828:CDS
CAAAATTCATTGGTGAAGCT+AGG 0.555453 3.3:+20139876 None:intergenic
AAAATTCTGGACTACTAATG+AGG 0.563972 3.3:-20141356 MS.gene46828:CDS
AAAGGATTAAATCAGCTACA+AGG 0.569777 3.3:+20138888 None:intergenic
GTAGAAAGCTTTAAAGCCAA+TGG 0.573198 3.3:-20140053 MS.gene46828:CDS
GAATCTGTTGCACAACAGAA+TGG 0.578310 3.3:-20141111 MS.gene46828:CDS
GAAGAATCTCTTACAAATGA+GGG 0.589972 3.3:-20141264 MS.gene46828:CDS
AGGTTTGTAAACAACGAGGT+TGG 0.591197 3.3:+20141321 None:intergenic
GGATCTGAGTTGATTTACCA+AGG 0.594347 3.3:-20141072 MS.gene46828:CDS
GACAGAATCAAGAAACTGCA+AGG 0.604322 3.3:-20139478 MS.gene46828:CDS
TGTAAACAACGAGGTTGGAG+TGG 0.605467 3.3:+20141326 None:intergenic
TGATATCAACTTAGAAAACA+TGG 0.611664 3.3:+20141209 None:intergenic
AATTTGGCAGCAAACAAGCA+CGG 0.620596 3.3:-20139122 MS.gene46828:intron
TGCAACATTGACATTGAGAG+AGG 0.628805 3.3:-20140075 MS.gene46828:CDS
GTCATCTTAGTAGAAGAACT+AGG 0.642089 3.3:+20139720 None:intergenic
AAGTTGATATCATCTAACAA+TGG 0.667564 3.3:-20141198 MS.gene46828:CDS
ATCCAAGAAGCATAGCAGAG+AGG 0.668254 3.3:-20139623 MS.gene46828:CDS
GATTAGGTTTGTAAACAACG+AGG 0.763241 3.3:+20141317 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTTAATCTGAAAATTTC+AGG - chr3.3:20140819-20140838 MS.gene46828:intron 20.0%
!!! AAAATACCTTATCTGATTTT+GGG + chr3.3:20140894-20140913 None:intergenic 20.0%
!!! ATAATGTTTTCTACTTCAAA+TGG - chr3.3:20140533-20140552 MS.gene46828:intron 20.0%
!!! CTTTTTCCTCTTAATAATTA+GGG - chr3.3:20139501-20139520 MS.gene46828:CDS 20.0%
!!! TAAAATACCTTATCTGATTT+TGG + chr3.3:20140895-20140914 None:intergenic 20.0%
!!! TGAATACTTTTATGAATTGA+AGG - chr3.3:20140239-20140258 MS.gene46828:intron 20.0%
!!! TTAGACATTATAGATTTTGA+TGG + chr3.3:20141348-20141367 None:intergenic 20.0%
!!! TTTAAAGTTATTACTGATGT+CGG + chr3.3:20141073-20141092 None:intergenic 20.0%
! AACTTGATTCATCATTAGTA+AGG - chr3.3:20139902-20139921 MS.gene46828:CDS 25.0%
! AATGGTAATTATTCTTATGG+TGG - chr3.3:20139247-20139266 MS.gene46828:intron 25.0%
! ACAAACCTAGTAAATGAAAA+TGG - chr3.3:20140422-20140441 MS.gene46828:intron 25.0%
! ACATAGGCTTAATAATAGTA+TGG + chr3.3:20140782-20140801 None:intergenic 25.0%
! AGAAAAAACAAATGAGCAAT+TGG - chr3.3:20139850-20139869 MS.gene46828:CDS 25.0%
! AGCTTATAAAAACAGCTTAT+AGG - chr3.3:20139656-20139675 MS.gene46828:CDS 25.0%
! ATGAAAAATATAAGCGCTAA+AGG - chr3.3:20139946-20139965 MS.gene46828:CDS 25.0%
! CAGAATGGTAATTATTCTTA+TGG - chr3.3:20139244-20139263 MS.gene46828:intron 25.0%
! CATTATCAACAACAAAATTC+TGG - chr3.3:20138971-20138990 MS.gene46828:intron 25.0%
! GTAACTTAGTACTTCTAATT+TGG - chr3.3:20141202-20141221 MS.gene46828:CDS 25.0%
! TCAAAATAAGTCAAACAGTT+TGG + chr3.3:20140214-20140233 None:intergenic 25.0%
! TCATTCAATGTGTTTATGTA+AGG + chr3.3:20141318-20141337 None:intergenic 25.0%
! TGATATCAACTTAGAAAACA+TGG + chr3.3:20139134-20139153 None:intergenic 25.0%
! TGCGTAGTTTATATACATTT+CGG - chr3.3:20141180-20141199 MS.gene46828:CDS 25.0%
!! AACACCAATTTTAGATTGAT+TGG + chr3.3:20139365-20139384 None:intergenic 25.0%
!! ATCAATCTAAAATTGGTGTT+AGG - chr3.3:20139365-20139384 MS.gene46828:intron 25.0%
!! CATTTACTAGGTTTGTATTT+TGG + chr3.3:20140418-20140437 None:intergenic 25.0%
!! CGCTTATATTTTTCATTTAC+AGG + chr3.3:20139942-20139961 None:intergenic 25.0%
!! GAGAATTTTCTTCAAATTCA+AGG - chr3.3:20140650-20140669 MS.gene46828:intron 25.0%
!! GAGTTTTTCTCTAATGAAAA+TGG - chr3.3:20139427-20139446 MS.gene46828:intron 25.0%
!! GATTCCAATCAATCTAAAAT+TGG - chr3.3:20139358-20139377 MS.gene46828:intron 25.0%
!!! AAGTTGATATCATCTAACAA+TGG - chr3.3:20139142-20139161 MS.gene46828:intron 25.0%
!!! ATTGCTCATTTGTTTTTTCT+TGG + chr3.3:20139850-20139869 None:intergenic 25.0%
!!! GCTTTTTCCTCTTAATAATT+AGG - chr3.3:20139500-20139519 MS.gene46828:CDS 25.0%
!!! TAATATATGAAGTACTGTCA+TGG - chr3.3:20140031-20140050 MS.gene46828:CDS 25.0%
!!! TTTGTATTTTGGTCACAATT+TGG + chr3.3:20140407-20140426 None:intergenic 25.0%
AAAATTCTGGACTACTAATG+AGG - chr3.3:20138984-20139003 MS.gene46828:intron 30.0%
AACAGACCCTAATTATTAAG+AGG + chr3.3:20139510-20139529 None:intergenic 30.0%
AATAACTCAATATCCGAACA+GGG - chr3.3:20139756-20139775 MS.gene46828:CDS 30.0%
AGAATTGTCCCAAAATTCAT+TGG + chr3.3:20140477-20140496 None:intergenic 30.0%
AGCTTATTGAGCTTATCTAT+TGG - chr3.3:20139536-20139555 MS.gene46828:intron 30.0%
ATGAAACTGTTGAATCACAT+AGG + chr3.3:20140798-20140817 None:intergenic 30.0%
CGGATATTGAGTTATTACAT+AGG + chr3.3:20139752-20139771 None:intergenic 30.0%
CTTAATAATTAGGGTCTGTT+TGG - chr3.3:20139510-20139529 MS.gene46828:intron 30.0%
GAAGAATCTCTTACAAATGA+GGG - chr3.3:20139076-20139095 MS.gene46828:CDS 30.0%
TAATAACTCAATATCCGAAC+AGG - chr3.3:20139755-20139774 MS.gene46828:CDS 30.0%
TACTTGAAGAATCCATTTCT+TGG + chr3.3:20138934-20138953 None:intergenic 30.0%
TGATATCATCTAACAATGGA+TGG - chr3.3:20139146-20139165 MS.gene46828:intron 30.0%
TTCATCATTAGTAAGGTTTC+AGG - chr3.3:20139909-20139928 MS.gene46828:CDS 30.0%
TTCTACTAAGATGACATCTA+TGG - chr3.3:20140628-20140647 MS.gene46828:intron 30.0%
! AATGGTTTTTATGGCTCTTT+TGG - chr3.3:20139310-20139329 MS.gene46828:intron 30.0%
! ACTCTTTTGCCTAATAAGAT+TGG + chr3.3:20139398-20139417 None:intergenic 30.0%
! AGCTACCATTTTCATTTACT+AGG + chr3.3:20140430-20140449 None:intergenic 30.0%
! ATGTCTTCAATCTAAAGTGT+CGG - chr3.3:20141154-20141173 MS.gene46828:CDS 30.0%
! ATTCCATTTTCAGCTATTTG+TGG + chr3.3:20140371-20140390 None:intergenic 30.0%
! ATTTACCAAGGCTTTTCAAA+TGG - chr3.3:20139280-20139299 MS.gene46828:intron 30.0%
! GTTTTCTACTTCAAATGGTT+TGG - chr3.3:20140538-20140557 MS.gene46828:intron 30.0%
! TAGCTTCACCAATGAATTTT+GGG - chr3.3:20140466-20140485 MS.gene46828:intron 30.0%
! TCTTTTCCCAAAATCAGATA+AGG - chr3.3:20140885-20140904 MS.gene46828:intron 30.0%
!! CTTTTGAGAAAGTTAAAGTC+TGG + chr3.3:20140983-20141002 None:intergenic 30.0%
!!! GATTTTGGCTATCAAAATCT+TGG - chr3.3:20140575-20140594 MS.gene46828:intron 30.0%
!!! GCTGTTTTTATAAGCTATCT+AGG + chr3.3:20139652-20139671 None:intergenic 30.0%
AAACCTGTGAAGCAAGAAAT+AGG - chr3.3:20139205-20139224 MS.gene46828:intron 35.0%
AATCTGTGAGTATCTGCAAA+AGG + chr3.3:20141385-20141404 None:intergenic 35.0%
AGTAACGTAGACACAGATAT+TGG - chr3.3:20141033-20141052 MS.gene46828:CDS 35.0%
ATGCCACAAATAGCTGAAAA+TGG - chr3.3:20140365-20140384 MS.gene46828:intron 35.0%
ATTAGAGAAAAACTCAGCAG+GGG + chr3.3:20139422-20139441 None:intergenic 35.0%
CAAAATTCATTGGTGAAGCT+AGG + chr3.3:20140467-20140486 None:intergenic 35.0%
CATTAGAGAAAAACTCAGCA+GGG + chr3.3:20139423-20139442 None:intergenic 35.0%
CGAAGAATCTCTTACAAATG+AGG - chr3.3:20139075-20139094 MS.gene46828:CDS 35.0%
CTTGATTCTGTCACTAATTC+TGG + chr3.3:20140854-20140873 None:intergenic 35.0%
CTTTAGATTGAAGACATGAC+CGG + chr3.3:20141151-20141170 None:intergenic 35.0%
GATTAGGTTTGTAAACAACG+AGG + chr3.3:20139026-20139045 None:intergenic 35.0%
GCTAAGAAGAAACCAAGAAA+TGG - chr3.3:20138919-20138938 MS.gene46828:CDS 35.0%
GGAATTGCTCCAATCTTATT+AGG - chr3.3:20139386-20139405 MS.gene46828:intron 35.0%
GTAAAATTCGAGCGAAAAGA+GGG - chr3.3:20140684-20140703 MS.gene46828:intron 35.0%
GTACACTTAACTTCTCATCT+AGG - chr3.3:20140321-20140340 MS.gene46828:intron 35.0%
GTCATCTTAGTAGAAGAACT+AGG + chr3.3:20140623-20140642 None:intergenic 35.0%
TCATTAGAGAAAAACTCAGC+AGG + chr3.3:20139424-20139443 None:intergenic 35.0%
TCCATCAACAAGTTCAGAAA+TGG - chr3.3:20139105-20139124 MS.gene46828:CDS 35.0%
TGAAAACATTAAGCCCTGTT+CGG + chr3.3:20139772-20139791 None:intergenic 35.0%
TGTAAAATTCGAGCGAAAAG+AGG - chr3.3:20140683-20140702 MS.gene46828:intron 35.0%
! CTAGCTTCACCAATGAATTT+TGG - chr3.3:20140465-20140484 MS.gene46828:intron 35.0%
! CTTTTCGCTCGAATTTTACA+AGG + chr3.3:20140683-20140702 None:intergenic 35.0%
!! AGTTTTGTCCGTTGTATGAA+CGG - chr3.3:20139969-20139988 MS.gene46828:CDS 35.0%
!! ATCCTTTTCAAGCTAGTAGA+TGG + chr3.3:20140347-20140366 None:intergenic 35.0%
!! GTAGAAAGCTTTAAAGCCAA+TGG - chr3.3:20140287-20140306 MS.gene46828:intron 35.0%
!! TATGAAGTACTGTCATGGAT+AGG - chr3.3:20140036-20140055 MS.gene46828:CDS 35.0%
!! TCCATTTCTGAACTTGTTGA+TGG + chr3.3:20139109-20139128 None:intergenic 35.0%
!!! TTTTCAAATGGTTCTGGCAA+TGG - chr3.3:20139292-20139311 MS.gene46828:intron 35.0%
!!! TTTTGGTGGAGTTGATACTA+TGG - chr3.3:20139327-20139346 MS.gene46828:intron 35.0%
AAAGCCAATGGCATCTCAAA+TGG - chr3.3:20140299-20140318 MS.gene46828:intron 40.0%
AAGCTATCTAGGAGAGCTTA+AGG + chr3.3:20139641-20139660 None:intergenic 40.0%
AATTTGGCAGCAAACAAGCA+CGG - chr3.3:20141218-20141237 MS.gene46828:CDS 40.0%
AGACCTACAAGAACAAGTTC+AGG - chr3.3:20141275-20141294 MS.gene46828:CDS 40.0%
AGGTTTGTAAACAACGAGGT+TGG + chr3.3:20139022-20139041 None:intergenic 40.0%
CAGAACCATTTGAAAAGCCT+TGG + chr3.3:20139288-20139307 None:intergenic 40.0%
GAATCTGTTGCACAACAGAA+TGG - chr3.3:20139229-20139248 MS.gene46828:intron 40.0%
GACAGAATCAAGAAACTGCA+AGG - chr3.3:20140862-20140881 MS.gene46828:intron 40.0%
GCAATTGGACCAATGTTTGT+TGG - chr3.3:20139865-20139884 MS.gene46828:CDS 40.0%
GCAGATACTCACAGATTGTA+AGG - chr3.3:20141388-20141407 MS.gene46828:CDS 40.0%
TATTGGCACACACACTTGTT+AGG - chr3.3:20139553-20139572 MS.gene46828:intron 40.0%
TGCAACATTGACATTGAGAG+AGG - chr3.3:20140265-20140284 MS.gene46828:intron 40.0%
! CAAGGCTTTTCAAATGGTTC+TGG - chr3.3:20139286-20139305 MS.gene46828:intron 40.0%
! GAAGCTAGGTATGTGACTTT+TGG + chr3.3:20140453-20140472 None:intergenic 40.0%
! GACTCTCAAGAAGCAGATTT+TGG - chr3.3:20140560-20140579 MS.gene46828:intron 40.0%
! GGTTTTTATGGCTCTTTTGG+TGG - chr3.3:20139313-20139332 MS.gene46828:intron 40.0%
! GTACCTGAACTTGTTCTTGT+AGG + chr3.3:20141281-20141300 None:intergenic 40.0%
! TCACCTATTTCTTGCTTCAC+AGG + chr3.3:20139211-20139230 None:intergenic 40.0%
! TGTACCATTTGAGATGCCAT+TGG + chr3.3:20140306-20140325 None:intergenic 40.0%
! TTTGAGCGTTGTTGTCGATT+AGG + chr3.3:20139042-20139061 None:intergenic 40.0%
!! GGATCTGAGTTGATTTACCA+AGG - chr3.3:20139268-20139287 MS.gene46828:intron 40.0%
!! GGTTCTGGCAATGGTTTTTA+TGG - chr3.3:20139301-20139320 MS.gene46828:intron 40.0%
AACAAGCACGGCAGATATGT+TGG - chr3.3:20141230-20141249 MS.gene46828:CDS 45.0%
AGACACCACTTGTCATACAC+CGG - chr3.3:20141129-20141148 MS.gene46828:CDS 45.0%
CACGGCAGATATGTTGGATT+TGG - chr3.3:20141236-20141255 MS.gene46828:CDS 45.0%
CTCCTTGGACCAACAAACAT+TGG + chr3.3:20139877-20139896 None:intergenic 45.0%
GAATCAAGTTCCTAGCTCCT+TGG + chr3.3:20139892-20139911 None:intergenic 45.0%
GACCATAGCCGTTCATACAA+CGG + chr3.3:20139980-20139999 None:intergenic 45.0%
TGTAAACAACGAGGTTGGAG+TGG + chr3.3:20139017-20139036 None:intergenic 45.0%
! ATCCAAGAAGCATAGCAGAG+AGG - chr3.3:20140717-20140736 MS.gene46828:intron 45.0%
! GACCAATGTTTGTTGGTCCA+AGG - chr3.3:20139872-20139891 MS.gene46828:CDS 45.0%
! GGCCATCTACTAGCTTGAAA+AGG - chr3.3:20140342-20140361 MS.gene46828:intron 45.0%
! TCCAAGAAGCATAGCAGAGA+GGG - chr3.3:20140718-20140737 MS.gene46828:intron 45.0%
ACCCTCTCTGCTATGCTTCT+TGG + chr3.3:20140722-20140741 None:intergenic 50.0%
CATGACCGGTGTATGACAAG+TGG + chr3.3:20141137-20141156 None:intergenic 50.0%
GTCCGTTGTATGAACGGCTA+TGG - chr3.3:20139975-20139994 MS.gene46828:CDS 50.0%
! GTTTGTTGGTCCAAGGAGCT+AGG - chr3.3:20139879-20139898 MS.gene46828:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 20138899 20141463 20138899 ID=MS.gene46828
chr3.3 mRNA 20138899 20141463 20138899 ID=MS.gene46828.t1;Parent=MS.gene46828
chr3.3 exon 20140914 20141463 20140914 ID=MS.gene46828.t1.exon1;Parent=MS.gene46828.t1
chr3.3 CDS 20140914 20141463 20140914 ID=cds.MS.gene46828.t1;Parent=MS.gene46828.t1
chr3.3 exon 20139623 20140101 20139623 ID=MS.gene46828.t1.exon2;Parent=MS.gene46828.t1
chr3.3 CDS 20139623 20140101 20139623 ID=cds.MS.gene46828.t1;Parent=MS.gene46828.t1
chr3.3 exon 20139456 20139521 20139456 ID=MS.gene46828.t1.exon3;Parent=MS.gene46828.t1
chr3.3 CDS 20139456 20139521 20139456 ID=cds.MS.gene46828.t1;Parent=MS.gene46828.t1
chr3.3 exon 20139066 20139134 20139066 ID=MS.gene46828.t1.exon4;Parent=MS.gene46828.t1
chr3.3 CDS 20139066 20139134 20139066 ID=cds.MS.gene46828.t1;Parent=MS.gene46828.t1
chr3.3 exon 20138899 20138970 20138899 ID=MS.gene46828.t1.exon5;Parent=MS.gene46828.t1
chr3.3 CDS 20138899 20138970 20138899 ID=cds.MS.gene46828.t1;Parent=MS.gene46828.t1
Gene Sequence

>MS.gene46828

ATGGGTGCTATGTATGAAGAGCTAAGAAGAAACCAAGAAATGGATTCTTCAAGTATTATTCATTCAAAACAACATTATCAACAACAAAATTCTGGACTACTAATGAGGTATAATTCCACTCCAACCTCGTTGTTTACAAACCTAATCGACAACAACGCTCAAAACTATATCAACAACGAAGAATCTCTTACAAATGAGGGTTATCTTCCATCAACAAGTTCAGAAATGGATACCATGTTTTCTAAGTTGATATCATCTAACAATGGATGGAACAATTCTGAAGAAAATTTGCAAGAGTTTGATGTGAAACCTGTGAAGCAAGAAATAGGTGAATCTGTTGCACAACAGAATGGTAATTATTCTTATGGTGGATCTGAGTTGATTTACCAAGGCTTTTCAAATGGTTCTGGCAATGGTTTTTATGGCTCTTTTGGTGGAGTTGATACTATGGAATCAGATGATTCCAATCAATCTAAAATTGGTGTTAGGAATTGCTCCAATCTTATTAGGCAAAAGAGTTCCCCTGCTGAGTTTTTCTCTAATGAAAATGGTATGAATTTTTTTCTTATTTGCAAAGTTTTATAGATTTTAGTTACTGTGTGCTTTTTCCTCTTAATAATTAGGGTCTGTTTGGATTAGCTTATTGAGCTTATCTATTGGCACACACACTTGTTAGGCTATTTTGACAAGCTTATGAAAATATCTTATGACATGTTTTTAAGTTGTTTTCATCTTTTTTCCTTAAGCTCTCCTAGATAGCTTATAAAAACAGCTTATAGGAAAACAAATTTGATTTTATTCTATCTTTTATTGTAGAAATAGCTTATGCATAAGAATTTATAGATTAACTCCTATGTAATAACTCAATATCCGAACAGGGCTTAATGTTTTCATATAGTTGAAAGATAAATGTTATGATTTAAATTGAAATACTTCTCTTGTATGAAACCAAGAAAAAACAAATGAGCAATTGGACCAATGTTTGTTGGTCCAAGGAGCTAGGAACTTGATTCATCATTAGTAAGGTTTCAGGTTTGAGTCCTGTAAATGAAAAATATAAGCGCTAAAGGAGTTTTGTCCGTTGTATGAACGGCTATGGTCATCTCTGTTAAATTATGAAAATGTTCAAAATTAATATATGAAGTACTGTCATGGATAGGACACTTGACATGACAATAACACTGATGAACGTGATTGAATGTAAGCACATGTGTTGACGTCGTGTCAGTATGAGATATTGACATGTGTCAGTGCTACACGTGAATTAACTACACTTTGAAAGTTATTTAGCATTAAATCAAATCAAATCAAACCAAACTGTTTGACTTATTTTGATATTTTGAATACTTTTATGAATTGAAGGACTTGCAACATTGACATTGAGAGAGGTAGAAAGCTTTAAAGCCAATGGCATCTCAAATGGTACACTTAACTTCTCATCTAGGCCATCTACTAGCTTGAAAAGGATGCCACAAATAGCTGAAAATGGAATTCAAAGCTTAGAACCAAATTGTGACCAAAATACAAACCTAGTAAATGAAAATGGTAGCTCCAAAAGTCACATACCTAGCTTCACCAATGAATTTTGGGACAATTCTGCATTCAATGCACACAAAACAGAGAGTGAAGATGAAATAATGTTTTCTACTTCAAATGGTTTGGACTCTCAAGAAGCAGATTTTGGCTATCAAAATCTTGGTTTGACTAGTCATCTGAGTCTTCCTAGTTCTTCTACTAAGATGACATCTATGGAGAATTTTCTTCAAATTCAAGGTTCTGTTCCTTGTAAAATTCGAGCGAAAAGAGGGTTTGCAACTCATCCAAGAAGCATAGCAGAGAGGGTAAAATTTACTAACAACTATAAATCTATCGTTACTCGACCATACTATTATTAAGCCTATGTGATTCAACAGTTTCATATATGTTAATCTGAAAATTTCAGGTCAGAAGAACCAGAATTAGTGACAGAATCAAGAAACTGCAAGGTCTTTTCCCAAAATCAGATAAGGTATTTTAAAGTCTGTTTATTTTCTAAACAAATTCGTAGTTTTTGTTTTTTTGAAATCGATGTTCATTTTAAACCAGACTTTAACTTTCTCAAAAGCATATCATATTGAAACTTCATATGCATTTTAGTAACGTAGACACAGATATTGGACATGACACTGATACCGACATCAGTAATAACTTTAAAAAATGAAAGTAATTGAATGTCACATGTGTCTGTCTCAGACACCACTTGTCATACACCGGTCATGTCTTCAATCTAAAGTGTCGGTGCTGCGTAGTTTATATACATTTCGGTAACTTAGTACTTCTAATTTGGCAGCAAACAAGCACGGCAGATATGTTGGATTTGGCAGTTGAGTACATTAAAGACCTACAAGAACAAGTTCAGGTACAAGAAAATTATATACCTTACATAAACACATTGAATGATTAAATTCCATCAAAATCTATAATGTCTAACTAAATTTCTTTCACCTTTTGCAGATACTCACAGATTGTAAGGAAAAATGCAAATGTGCAAGTCATGAGAAGAAACACTGTAAACCTTGTAGCTGA
Protein sequence

>MS.gene46828.t1

MGAMYEELRRNQEMDSSSIIHSKQHYQQQNSGLLMRYNSTPTSLFTNLIDNNAQNYINNEESLTNEGYLPSTSSEMDTMFSKLISSNNGWNNSEENLQEFDVKPVKQEIGESVAQQNGNYSYGGSELIYQGFSNGSGNGFYGSFGGVDTMESDDSNQSKIGVRNCSNLIRQKSSPAEFFSNENGLATLTLREVESFKANGISNGTLNFSSRPSTSLKRMPQIAENGIQSLEPNCDQNTNLVNENGSSKSHIPSFTNEFWDNSAFNAHKTESEDEIMFSTSNGLDSQEADFGYQNLGLTSHLSLPSSSTKMTSMENFLQIQGSVPCKIRAKRGFATHPRSIAERVRRTRISDRIKKLQGLFPKSDKQTSTADMLDLAVEYIKDLQEQVQILTDCKEKCKCASHEKKHCKPCS