Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47044.t1 | XP_003618052.1 | 74.6 | 248 | 59 | 2 | 1 | 248 | 27 | 270 | 1.60E-79 | 306.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47044.t1 | G7K0M6 | 74.6 | 248 | 59 | 2 | 1 | 248 | 27 | 270 | 1.1e-79 | 306.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene47044.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47044.t1 | MTR_5g098470 | 75.000 | 248 | 58 | 2 | 1 | 248 | 27 | 270 | 2.16e-129 | 369 |
| MS.gene47044.t1 | MTR_1g046810 | 30.000 | 300 | 132 | 16 | 36 | 288 | 37 | 305 | 6.78e-13 | 68.2 |
| MS.gene47044.t1 | MTR_6g081160 | 28.283 | 198 | 119 | 6 | 40 | 227 | 30 | 214 | 4.23e-12 | 65.5 |
| MS.gene47044.t1 | MTR_1g052185 | 27.055 | 292 | 161 | 10 | 19 | 288 | 9 | 270 | 1.50e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47044.t1 | AT5G56200 | 46.429 | 84 | 41 | 1 | 38 | 117 | 77 | 160 | 7.37e-14 | 72.0 |
Find 45 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACGATGATTCAAAATTT+TGG | 0.150603 | 5.1:+69901010 | MS.gene47044:CDS |
| TCAACATGATGATGACTATT+TGG | 0.187915 | 5.1:-69900643 | None:intergenic |
| ATACCCCTCCATTCCCTTTC+AGG | 0.219356 | 5.1:-69900898 | None:intergenic |
| GTGACCATATAAGGCCTTAT+TGG | 0.260984 | 5.1:-69900863 | None:intergenic |
| CTTCTTCTCTATGGATTTCA+TGG | 0.270549 | 5.1:-69900569 | None:intergenic |
| TTTAGGTGTGGAAAAGCATT+AGG | 0.279524 | 5.1:+69900715 | MS.gene47044:CDS |
| CCATCCAATAAGGCCTTATA+TGG | 0.323281 | 5.1:+69900859 | MS.gene47044:CDS |
| AGATCTCATGTGACCATATA+AGG | 0.346698 | 5.1:-69900872 | None:intergenic |
| TGCAATTCTTCTAATGATAG+TGG | 0.351010 | 5.1:+69900802 | MS.gene47044:CDS |
| GATCCCATCTTCAAGCTCTA+AGG | 0.358194 | 5.1:+69900749 | MS.gene47044:CDS |
| AAAGAATTTCCCATCCAATA+AGG | 0.359792 | 5.1:+69900849 | MS.gene47044:CDS |
| GCAAAGAGATGAATTGTAAT+AGG | 0.364306 | 5.1:+69901346 | MS.gene47044:CDS |
| GAGGGGTATGTATCCTAATA+AGG | 0.367738 | 5.1:+69900912 | MS.gene47044:CDS |
| TTAGGTGTGGAAAAGCATTA+GGG | 0.371642 | 5.1:+69900716 | MS.gene47044:CDS |
| CCTAATAAGGATAAAACTAT+TGG | 0.385116 | 5.1:+69900925 | MS.gene47044:CDS |
| CATCATGTTGAAGGATCAAC+AGG | 0.420630 | 5.1:+69900655 | MS.gene47044:CDS |
| CTTTCCTTAGAGCTTGAAGA+TGG | 0.421663 | 5.1:-69900753 | None:intergenic |
| CCTGGTACAAGTGGTCTTGT+TGG | 0.425426 | 5.1:+69901186 | MS.gene47044:CDS |
| AATAGTCATCATCATGTTGA+AGG | 0.464152 | 5.1:+69900646 | MS.gene47044:CDS |
| TACCCCTCCATTCCCTTTCA+GGG | 0.465209 | 5.1:-69900897 | None:intergenic |
| CCACCAATATGGTTTAAAAC+CGG | 0.477481 | 5.1:+69901033 | MS.gene47044:CDS |
| CCATATAAGGCCTTATTGGA+TGG | 0.492046 | 5.1:-69900859 | None:intergenic |
| CATGAGATCTCACCCTGAAA+GGG | 0.502988 | 5.1:+69900885 | MS.gene47044:CDS |
| TGCCATCATAAGTTCCACTT+TGG | 0.505420 | 5.1:-69901326 | None:intergenic |
| GATGAAACAAAATTGAAGAA+TGG | 0.505465 | 5.1:+69901216 | MS.gene47044:CDS |
| TTCGGATGACGAGGATAAGA+AGG | 0.520435 | 5.1:+69901152 | MS.gene47044:CDS |
| ACATGAGATCTCACCCTGAA+AGG | 0.521597 | 5.1:+69900884 | MS.gene47044:CDS |
| TGGTTTAAAACCGGCAAAAG+AGG | 0.523461 | 5.1:+69901042 | MS.gene47044:CDS |
| TGACAACTTCAATGCAGATT+CGG | 0.527965 | 5.1:+69901134 | MS.gene47044:CDS |
| AGAATGGCAATAACAAAAGA+AGG | 0.539915 | 5.1:+69901232 | MS.gene47044:CDS |
| TACCAAAGTGGAACTTATGA+TGG | 0.540351 | 5.1:+69901324 | MS.gene47044:CDS |
| CCAACAAGACCACTTGTACC+AGG | 0.547377 | 5.1:-69901186 | None:intergenic |
| TTTCCTTAGAGCTTGAAGAT+GGG | 0.556262 | 5.1:-69900752 | None:intergenic |
| TCTTCAAGCTCTAAGGAAAG+AGG | 0.559146 | 5.1:+69900756 | MS.gene47044:CDS |
| CATATAAGGCCTTATTGGAT+GGG | 0.559243 | 5.1:-69900858 | None:intergenic |
| GATCTCACCCTGAAAGGGAA+TGG | 0.563550 | 5.1:+69900890 | MS.gene47044:CDS |
| TAACAAAAGAAGGAAGAAGC+TGG | 0.580002 | 5.1:+69901242 | MS.gene47044:CDS |
| TCACCCTGAAAGGGAATGGA+GGG | 0.580204 | 5.1:+69900894 | MS.gene47044:CDS |
| CTCACCCTGAAAGGGAATGG+AGG | 0.583418 | 5.1:+69900893 | MS.gene47044:CDS |
| CTTCAAGCTCTAAGGAAAGA+GGG | 0.588989 | 5.1:+69900757 | MS.gene47044:CDS |
| CAATGCAGATTCGGATGACG+AGG | 0.631053 | 5.1:+69901143 | MS.gene47044:CDS |
| AGGTGTGGAAAAGCATTAGG+GGG | 0.631959 | 5.1:+69900718 | MS.gene47044:CDS |
| TAGGTGTGGAAAAGCATTAG+GGG | 0.641738 | 5.1:+69900717 | MS.gene47044:CDS |
| CACCCTGAAAGGGAATGGAG+GGG | 0.655548 | 5.1:+69900895 | MS.gene47044:CDS |
| AGAGGGTGAGTTGAAAACAA+AGG | 0.686503 | 5.1:+69900774 | MS.gene47044:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGTAACAAAATTTTT+AGG | + | chr5.1:69900698-69900717 | MS.gene47044:CDS | 10.0% |
| !!! | TTGTTTTTAGATATTGAACA+TGG | - | chr5.1:69901282-69901301 | None:intergenic | 20.0% |
| ! | ATCTAAAAACAACATCTTGT+TGG | + | chr5.1:69901290-69901309 | MS.gene47044:CDS | 25.0% |
| ! | CCTAATAAGGATAAAACTAT+TGG | + | chr5.1:69900925-69900944 | MS.gene47044:CDS | 25.0% |
| ! | GATGAAACAAAATTGAAGAA+TGG | + | chr5.1:69901216-69901235 | MS.gene47044:CDS | 25.0% |
| !! | CAAATCTTTTGAATTGATTG+CGG | - | chr5.1:69900986-69901005 | None:intergenic | 25.0% |
| !! | TAAACTTTTCTTCTTCTCTA+TGG | - | chr5.1:69900581-69900600 | None:intergenic | 25.0% |
| !! | TTGACGATGATTCAAAATTT+TGG | + | chr5.1:69901010-69901029 | MS.gene47044:CDS | 25.0% |
| !!! | CCAATAGTTTTATCCTTATT+AGG | - | chr5.1:69900928-69900947 | None:intergenic | 25.0% |
| !!! | TGTAACAAAATTTTTAGGTG+TGG | + | chr5.1:69900703-69900722 | MS.gene47044:CDS | 25.0% |
| AAAGAATTTCCCATCCAATA+AGG | + | chr5.1:69900849-69900868 | MS.gene47044:CDS | 30.0% | |
| AATAGTCATCATCATGTTGA+AGG | + | chr5.1:69900646-69900665 | MS.gene47044:CDS | 30.0% | |
| AGAATGGCAATAACAAAAGA+AGG | + | chr5.1:69901232-69901251 | MS.gene47044:CDS | 30.0% | |
| GCAAAGAGATGAATTGTAAT+AGG | + | chr5.1:69901346-69901365 | MS.gene47044:CDS | 30.0% | |
| TGCAATTCTTCTAATGATAG+TGG | + | chr5.1:69900802-69900821 | MS.gene47044:CDS | 30.0% | |
| ! | AAGAAGGAGAATATTTTACC+TGG | + | chr5.1:69901168-69901187 | MS.gene47044:CDS | 30.0% |
| ! | AATATTTTACCTGGTACAAG+TGG | + | chr5.1:69901177-69901196 | MS.gene47044:CDS | 30.0% |
| ! | TCAACATGATGATGACTATT+TGG | - | chr5.1:69900646-69900665 | None:intergenic | 30.0% |
| !!! | GTAATAGGCATTTTGAGATT+GGG | + | chr5.1:69901361-69901380 | MS.gene47044:CDS | 30.0% |
| !!! | TAATAGGCATTTTGAGATTG+GGG | + | chr5.1:69901362-69901381 | MS.gene47044:CDS | 30.0% |
| !!! | TGTAATAGGCATTTTGAGAT+TGG | + | chr5.1:69901360-69901379 | MS.gene47044:CDS | 30.0% |
| AGATCTCATGTGACCATATA+AGG | - | chr5.1:69900875-69900894 | None:intergenic | 35.0% | |
| GAAAAAGATTGCTACCAAAG+TGG | + | chr5.1:69901312-69901331 | MS.gene47044:CDS | 35.0% | |
| TAACAAAAGAAGGAAGAAGC+TGG | + | chr5.1:69901242-69901261 | MS.gene47044:CDS | 35.0% | |
| TACCAAAGTGGAACTTATGA+TGG | + | chr5.1:69901324-69901343 | MS.gene47044:CDS | 35.0% | |
| TGACAACTTCAATGCAGATT+CGG | + | chr5.1:69901134-69901153 | MS.gene47044:CDS | 35.0% | |
| TTTCCTTAGAGCTTGAAGAT+GGG | - | chr5.1:69900755-69900774 | None:intergenic | 35.0% | |
| ! | CCACCAATATGGTTTAAAAC+CGG | + | chr5.1:69901033-69901052 | MS.gene47044:CDS | 35.0% |
| !! | CAAAATTTTGGCCACCAATA+TGG | + | chr5.1:69901022-69901041 | MS.gene47044:CDS | 35.0% |
| !! | CATATAAGGCCTTATTGGAT+GGG | - | chr5.1:69900861-69900880 | None:intergenic | 35.0% |
| !! | TTAGGTGTGGAAAAGCATTA+GGG | + | chr5.1:69900716-69900735 | MS.gene47044:CDS | 35.0% |
| !! | TTTAGGTGTGGAAAAGCATT+AGG | + | chr5.1:69900715-69900734 | MS.gene47044:CDS | 35.0% |
| !!! | TTGCCGGTTTTAAACCATAT+TGG | - | chr5.1:69901039-69901058 | None:intergenic | 35.0% |
| CATCATGTTGAAGGATCAAC+AGG | + | chr5.1:69900655-69900674 | MS.gene47044:CDS | 40.0% | |
| CTTTCCTTAGAGCTTGAAGA+TGG | - | chr5.1:69900756-69900775 | None:intergenic | 40.0% | |
| TGCCATCATAAGTTCCACTT+TGG | - | chr5.1:69901329-69901348 | None:intergenic | 40.0% | |
| ! | AGAGGGTGAGTTGAAAACAA+AGG | + | chr5.1:69900774-69900793 | MS.gene47044:CDS | 40.0% |
| ! | CCATCCAATAAGGCCTTATA+TGG | + | chr5.1:69900859-69900878 | MS.gene47044:CDS | 40.0% |
| ! | GAGGGGTATGTATCCTAATA+AGG | + | chr5.1:69900912-69900931 | MS.gene47044:CDS | 40.0% |
| ! | GTGACCATATAAGGCCTTAT+TGG | - | chr5.1:69900866-69900885 | None:intergenic | 40.0% |
| ! | TGGTTTAAAACCGGCAAAAG+AGG | + | chr5.1:69901042-69901061 | MS.gene47044:CDS | 40.0% |
| !! | CCATATAAGGCCTTATTGGA+TGG | - | chr5.1:69900862-69900881 | None:intergenic | 40.0% |
| !! | CTTCAAGCTCTAAGGAAAGA+GGG | + | chr5.1:69900757-69900776 | MS.gene47044:CDS | 40.0% |
| !! | TAGGTGTGGAAAAGCATTAG+GGG | + | chr5.1:69900717-69900736 | MS.gene47044:CDS | 40.0% |
| !! | TCTTCAAGCTCTAAGGAAAG+AGG | + | chr5.1:69900756-69900775 | MS.gene47044:CDS | 40.0% |
| !!! | CCGGTTTTAAACCATATTGG+TGG | - | chr5.1:69901036-69901055 | None:intergenic | 40.0% |
| ACATGAGATCTCACCCTGAA+AGG | + | chr5.1:69900884-69900903 | MS.gene47044:CDS | 45.0% | |
| CATGAGATCTCACCCTGAAA+GGG | + | chr5.1:69900885-69900904 | MS.gene47044:CDS | 45.0% | |
| GATCCCATCTTCAAGCTCTA+AGG | + | chr5.1:69900749-69900768 | MS.gene47044:CDS | 45.0% | |
| TTCGGATGACGAGGATAAGA+AGG | + | chr5.1:69901152-69901171 | MS.gene47044:CDS | 45.0% | |
| !! | AGGTGTGGAAAAGCATTAGG+GGG | + | chr5.1:69900718-69900737 | MS.gene47044:CDS | 45.0% |
| ATACCCCTCCATTCCCTTTC+AGG | - | chr5.1:69900901-69900920 | None:intergenic | 50.0% | |
| CAATGCAGATTCGGATGACG+AGG | + | chr5.1:69901143-69901162 | MS.gene47044:CDS | 50.0% | |
| CCAACAAGACCACTTGTACC+AGG | - | chr5.1:69901189-69901208 | None:intergenic | 50.0% | |
| GATCTCACCCTGAAAGGGAA+TGG | + | chr5.1:69900890-69900909 | MS.gene47044:CDS | 50.0% | |
| TACCCCTCCATTCCCTTTCA+GGG | - | chr5.1:69900900-69900919 | None:intergenic | 50.0% | |
| TCACCCTGAAAGGGAATGGA+GGG | + | chr5.1:69900894-69900913 | MS.gene47044:CDS | 50.0% | |
| ! | GCTAGAACGTCCTCTTTTGC+CGG | - | chr5.1:69901055-69901074 | None:intergenic | 50.0% |
| !! | CCTGGTACAAGTGGTCTTGT+TGG | + | chr5.1:69901186-69901205 | MS.gene47044:CDS | 50.0% |
| CACCCTGAAAGGGAATGGAG+GGG | + | chr5.1:69900895-69900914 | MS.gene47044:CDS | 55.0% | |
| CTCACCCTGAAAGGGAATGG+AGG | + | chr5.1:69900893-69900912 | MS.gene47044:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 69900571 | 69901437 | 69900571 | ID=MS.gene47044 |
| chr5.1 | mRNA | 69900571 | 69901437 | 69900571 | ID=MS.gene47044.t1;Parent=MS.gene47044 |
| chr5.1 | exon | 69900571 | 69901437 | 69900571 | ID=MS.gene47044.t1.exon1;Parent=MS.gene47044.t1 |
| chr5.1 | CDS | 69900571 | 69901437 | 69900571 | ID=cds.MS.gene47044.t1;Parent=MS.gene47044.t1 |
| Gene Sequence |
| Protein sequence |
