Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47261.t1 | XP_013451506.1 | 82.5 | 326 | 34 | 3 | 1 | 321 | 1 | 308 | 8.90E-140 | 506.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47261.t1 | Q9SEZ4 | 57.6 | 118 | 50 | 0 | 89 | 206 | 104 | 221 | 6.7e-35 | 149.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47261.t1 | A0A072U6Z3 | 82.5 | 326 | 34 | 3 | 1 | 321 | 1 | 308 | 6.4e-140 | 506.5 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47261.t1 | MTR_6g027360 | 84.049 | 326 | 29 | 3 | 1 | 321 | 1 | 308 | 0.0 | 529 |
| MS.gene47261.t1 | MTR_6g027340 | 73.061 | 245 | 26 | 1 | 77 | 321 | 1 | 205 | 3.03e-121 | 347 |
| MS.gene47261.t1 | MTR_6g027370 | 57.923 | 366 | 90 | 10 | 1 | 321 | 1 | 347 | 1.79e-116 | 340 |
| MS.gene47261.t1 | MTR_7g086960 | 45.149 | 268 | 105 | 7 | 91 | 319 | 88 | 352 | 2.04e-49 | 168 |
| MS.gene47261.t1 | MTR_3g089045 | 64.423 | 104 | 37 | 0 | 92 | 195 | 16 | 119 | 1.02e-42 | 149 |
| MS.gene47261.t1 | MTR_4g082040 | 57.895 | 114 | 48 | 0 | 90 | 203 | 103 | 216 | 1.77e-42 | 152 |
| MS.gene47261.t1 | MTR_6g074860 | 55.752 | 113 | 50 | 0 | 90 | 202 | 2 | 114 | 2.28e-42 | 147 |
| MS.gene47261.t1 | MTR_5g041570 | 51.111 | 135 | 62 | 2 | 79 | 213 | 124 | 254 | 6.63e-42 | 150 |
| MS.gene47261.t1 | MTR_4g019370 | 51.579 | 95 | 46 | 0 | 92 | 186 | 38 | 132 | 9.15e-31 | 119 |
| MS.gene47261.t1 | MTR_3g463400 | 45.133 | 113 | 62 | 0 | 77 | 189 | 118 | 230 | 1.59e-27 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47261.t1 | AT4G33450 | 37.607 | 234 | 135 | 4 | 92 | 319 | 19 | 247 | 2.02e-48 | 162 |
| MS.gene47261.t1 | AT1G69560 | 57.627 | 118 | 50 | 0 | 90 | 207 | 105 | 222 | 1.44e-43 | 152 |
| MS.gene47261.t1 | AT1G69560 | 57.143 | 119 | 51 | 0 | 89 | 207 | 120 | 238 | 1.57e-43 | 153 |
| MS.gene47261.t1 | AT1G26780 | 55.469 | 128 | 56 | 1 | 90 | 217 | 96 | 222 | 2.08e-42 | 148 |
| MS.gene47261.t1 | AT1G26780 | 55.469 | 128 | 56 | 1 | 90 | 217 | 96 | 222 | 7.58e-42 | 149 |
| MS.gene47261.t1 | AT1G17950 | 58.491 | 106 | 44 | 0 | 90 | 195 | 3 | 108 | 2.57e-41 | 144 |
| MS.gene47261.t1 | AT5G17800 | 53.659 | 123 | 56 | 1 | 74 | 195 | 74 | 196 | 7.49e-38 | 137 |
| MS.gene47261.t1 | AT1G73410 | 59.048 | 105 | 43 | 0 | 91 | 195 | 5 | 109 | 3.85e-37 | 133 |
| MS.gene47261.t1 | AT1G73410 | 59.048 | 105 | 43 | 0 | 91 | 195 | 5 | 109 | 4.02e-37 | 132 |
| MS.gene47261.t1 | AT3G29020 | 53.097 | 113 | 53 | 0 | 91 | 203 | 64 | 176 | 1.21e-36 | 132 |
Find 68 sgRNAs with CRISPR-Local
Find 176 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTCCAAAATACCCTTT+GGG | 0.428010 | 6.3:+18735124 | MS.gene47261:CDS |
| AAAAGTTCAAAATACCCTTT+GGG | 0.385798 | 6.3:+18735070 | MS.gene47261:CDS |
| AAACAACTTATCGCTGACCA+TGG | 0.667532 | 6.3:+18735264 | MS.gene47261:CDS |
| AACTAGGTAAAGGCCTAGTG+CGG | 0.675780 | 6.3:-18735965 | None:intergenic |
| AACTTGAACAATGATGGTTA+TGG | 0.427382 | 6.3:+18734967 | MS.gene47261:CDS |
| AATCCAATCTCAACCGCACT+AGG | 0.519569 | 6.3:+18735952 | MS.gene47261:CDS |
| ACCATGACTCGAAATCCCAA+AGG | 0.646290 | 6.3:-18735085 | None:intergenic |
| ACCTTTAAGTAAGGAAGAAG+AGG | 0.519621 | 6.3:+18735722 | MS.gene47261:CDS |
| ACTATCATCTATTGCAAGAA+AGG | 0.451301 | 6.3:+18735875 | MS.gene47261:CDS |
| ACTCGCCTTCAGGGTCATGT+TGG | 0.594916 | 6.3:+18735033 | MS.gene47261:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGTCCAAAATACCCTTT+GGG | + | chr6.3:18735124-18735143 | MS.gene47261:CDS | 30.0% | |
| AACATTGCTGAAAAACTACA+AGG | + | chr6.3:18735303-18735322 | MS.gene47261:CDS | 30.0% | |
| AACTTGAACAATGATGGTTA+TGG | + | chr6.3:18734967-18734986 | MS.gene47261:CDS | 30.0% | |
| ACTATCATCTATTGCAAGAA+AGG | + | chr6.3:18735875-18735894 | MS.gene47261:CDS | 30.0% | |
| AGGCTTACTTCAAAAAAGAA+TGG | + | chr6.3:18737551-18737570 | MS.gene47261:intron | 30.0% | |
| ATAAACTAAGACATGAGAGA+AGG | - | chr6.3:18736187-18736206 | None:intergenic | 30.0% | |
| ATACACTTCATGGTAACAAA+TGG | + | chr6.3:18735763-18735782 | MS.gene47261:CDS | 30.0% | |
| ATGAGAATCGTTGTTAAATG+AGG | - | chr6.3:18736132-18736151 | None:intergenic | 30.0% | |
| CAAAAGAGAACCTTTAAGTA+AGG | + | chr6.3:18735713-18735732 | MS.gene47261:CDS | 30.0% | |
| CATGAAAATTGCAATCCATA+TGG | - | chr6.3:18737032-18737051 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 18734955 | 18737809 | 18734955 | ID=MS.gene47261 |
| chr6.3 | mRNA | 18734955 | 18737809 | 18734955 | ID=MS.gene47261.t1;Parent=MS.gene47261 |
| chr6.3 | exon | 18734955 | 18735333 | 18734955 | ID=MS.gene47261.t1.exon1;Parent=MS.gene47261.t1 |
| chr6.3 | CDS | 18734955 | 18735333 | 18734955 | ID=cds.MS.gene47261.t1;Parent=MS.gene47261.t1 |
| chr6.3 | exon | 18735667 | 18735996 | 18735667 | ID=MS.gene47261.t1.exon2;Parent=MS.gene47261.t1 |
| chr6.3 | CDS | 18735667 | 18735996 | 18735667 | ID=cds.MS.gene47261.t1;Parent=MS.gene47261.t1 |
| chr6.3 | exon | 18737553 | 18737809 | 18737553 | ID=MS.gene47261.t1.exon3;Parent=MS.gene47261.t1 |
| chr6.3 | CDS | 18737553 | 18737809 | 18737553 | ID=cds.MS.gene47261.t1;Parent=MS.gene47261.t1 |
| Gene Sequence |
>MS.gene47261 ATGAGTTCATTAAACTTGAACAATGATGGTTATGGTTTGTCTTTGGACTTGAAAATTTCTCTTCCATCATCATCTTCTACTCGCCTTCAGGGTCATGTTGGTAACAATGGTTTTGAAAAGTTCAAAATACCCTTTGGGATTTCGAGTCATGGTGGAATCAATGAGTCTGAAAAGTCCAAAATACCCTTTGGGATTTCAAACCCTCTGGTGATCCAACCTAATGAGAGCATGGTCCAAAAAAATGGGAGGAGTTTTAGGAACACTAACCTTTCCAAAGCTCATTGGAAACCATCTGAAGATGATAAACTTAAACAACTTATCGCTGACCATGGTGCTAAAGATTGGAACAACATTGCTGAAAAACTACAAGGAAGATCGGGTCTGATCCTACACTACCTAACAAATTTTTATTTATCAAAATTTCAATAAGATTATTAATTTATTTTTTAACCAAATTGTTTTTTTTAATATTGTTAATAATTTATATTGAATTTATTTATTTTTTACAAAAGTAAATATTCAAAAAACTGAACATTTTTCTCTCAAAAAAAAAAAAAAAAAAAAAACTGAACATTTTCAAGCCGTACGCTTAGTATTTTCATTAAAATTGCATAAGTATTGTATTTTGATTATAAATTTCTTTAATTTCAATATTTGTTTGTAATTTAACATTTAGTTTAGTTTTTTTTTTTTGTGATAATGGTAATTGCAGGGAAAATTTGTATATTAAGGTGGCTTAATCAACTAGATCCTAGCATCAAAAGAGAACCTTTAAGTAAGGAAGAAGAGGAGATGCTTTTGCAGCTTCATACACTTCATGGTAACAAATGGGCACACATTTGCAAACATTTTCCTGGAAGGACAGACAATGCACTAAAGAATAATTTCCATGTGATCAAGGCTAGCAGGCAAAGGGAACTACTATCATCTATTGCAAGAAAGGAAAAGCTAACATATGAAATCCCAGCAAAATTTGCCATCACAAGCCCCATTGATGAATCCAATCTCAACCGCACTAGGCCTTTACCTAGTTCTCAAATGGGTATACTTTGAATCCTTTATTTTATCTTATCAAGCAATTTTAAATATTGGTGCGCGGCTAGAACAATTATTGCAATATCATATTTTTTTATTTTTTTTTATGTCTTTGCATCTTTGATTGAAGCTGCATCGGCCTCATTTAACAACGATTCTCATTTAAACCTTTAAATTGCTTGAAAATTTTGTTACCTTCTCTCATGTCTTAGTTTATGTTAAATATCATATATTAATTTACTTGACATATGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGGGGGCTGTTAACTTGCACCCATCTAAAAACACTAAGGTGCAAGTTAACAAGCTGCACCTACTTGTACTTTACTAAAACACTATTTTGTCTCTATTAAACTTTATTTTAATTAAAAGGACATTTTTGTAAATAGCATAAATAATATTTGTTTATAATAGAAAAACATGGGTGTTTTGGTAAATAACTAGTAGGTGCTGCTTGTTAACTTGCACCTACTTGTACTTTACTAAAACACCATTTTGTCTCTATTAAACTTTATTTTAATTAATAGGACATTTTTGTAAATAGCATAAATAATATTTGTTTATAATAGAAAAACATGGGTGTTTTAGTAAATAACTAGTAGGTGCAGCTTGTTAACTTGCACCTTAGTGTTTTTAGGTGGGTGCAAGTTAACAGCCCCCATATATGAGACAGGATCCGTTGACACCTGTGTCAACGGATTCGTTGACACCAAATCTCAACCGTACATTATATTTAATCAAAGGCTCACAATAGTTTTTAAATTAACTCAGTGTGTGCACCCTTTCTCTCAATCTCACACTATTATGGTTTTCAACCATTCAAAAACTCAGATTGCATACATGATGCATCATGATTTATTCTTGTTCACATCTCCATATATTCTCTCTTGGTTAATTCCTCAAATTGAATTGCTGCCAATTCCTGGTGTACAAACAAAAAATCAAGAGTTATCTATCCCATCCCATATGGATTGCAATTTTCATGAGATCGATCCCAATTTGAGGAAGCTAGTGAAACTAGGGTAATTTAGATAAGTTTTTTTTACTACCGAATGGATTTTTGGTATCGAAATTGAAACAAGTGAGAAAAAACATCATTTCGAGGAATCTGATTTTGAATTTTAGCATATGATTTGTTTCAATTGATCACATAGAAAACCATTTAATTAATAACATAGAAAACCCTTTAAATTAAATTAAGTAGAAACGTGATTTCATGGAACTTTGGTGTGTGTTGATGGCAGATGGAGTATAAAAACGAGTTGCAATCAGATGAGTTGCTGGGAAAAATCACACAAATTAATTTAGCAAATAATTACTAACCATTAGATTAAATAAAATAAACGGCTCAGATTTGGTGTCAACGAATCCATTGACACCTGGTGTCAACGGATCCTGACTATATATATATATATATATATATATATATATATATATATATATATATGACCATATTGTTGATTTTTTTATTGTGTTATATTTTTAGGCTTACTTCAAAAAAGAATGGTAGAAGCTGAATTTGCCAAAGCTTGCAGTGCTATGAGAGAGCTTGACAATGTGTACCAGATGGAAAAGCTTAAGATTGCGGATCAATCCAATCTTTCAGATTCAAACTTAGAAGGTTCAACATCTGAGTCAGCTGCAACAAACATGAACAATGGATCAATTTTGGGGGATAGTGAAAATGTGGGAGATAAGATTAAGATGTCATTTTTTGACTTTCTTGGAGTAGGTGATACATAG |
| Protein sequence |
>MS.gene47261.t1 MSSLNLNNDGYGLSLDLKISLPSSSSTRLQGHVGNNGFEKFKIPFGISSHGGINESEKSKIPFGISNPLVIQPNESMVQKNGRSFRNTNLSKAHWKPSEDDKLKQLIADHGAKDWNNIAEKLQGRSGKICILRWLNQLDPSIKREPLSKEEEEMLLQLHTLHGNKWAHICKHFPGRTDNALKNNFHVIKASRQRELLSSIARKEKLTYEIPAKFAITSPIDESNLNRTRPLPSSQMGLLQKRMVEAEFAKACSAMRELDNVYQMEKLKIADQSNLSDSNLEGSTSESAATNMNNGSILGDSENVGDKIKMSFFDFLGVGDT |
