Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47262.t1 | XP_013451507.1 | 95.5 | 267 | 12 | 0 | 1 | 267 | 81 | 347 | 1.80E-141 | 511.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47262.t1 | Q9SEZ4 | 50.5 | 192 | 81 | 4 | 13 | 193 | 100 | 288 | 1.3e-44 | 181.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47262.t1 | A0A072UI90 | 95.5 | 267 | 12 | 0 | 1 | 267 | 81 | 347 | 1.3e-141 | 511.9 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47262.t1 | MTR_6g027370 | 95.506 | 267 | 12 | 0 | 1 | 267 | 81 | 347 | 0.0 | 505 |
| MS.gene47262.t1 | MTR_6g027360 | 61.004 | 259 | 78 | 8 | 11 | 267 | 71 | 308 | 3.80e-94 | 280 |
| MS.gene47262.t1 | MTR_6g027340 | 54.475 | 257 | 57 | 6 | 11 | 267 | 9 | 205 | 1.72e-81 | 244 |
| MS.gene47262.t1 | MTR_7g086960 | 54.945 | 273 | 106 | 4 | 10 | 265 | 80 | 352 | 6.54e-75 | 232 |
| MS.gene47262.t1 | MTR_4g082040 | 68.333 | 120 | 38 | 0 | 12 | 131 | 98 | 217 | 6.63e-57 | 188 |
| MS.gene47262.t1 | MTR_5g041570 | 51.397 | 179 | 78 | 3 | 5 | 183 | 122 | 291 | 4.01e-54 | 181 |
| MS.gene47262.t1 | MTR_6g074860 | 70.192 | 104 | 31 | 0 | 19 | 122 | 4 | 107 | 1.16e-51 | 169 |
| MS.gene47262.t1 | MTR_3g089045 | 70.909 | 110 | 32 | 0 | 13 | 122 | 10 | 119 | 2.36e-51 | 170 |
| MS.gene47262.t1 | MTR_4g019370 | 54.902 | 102 | 46 | 0 | 18 | 119 | 37 | 138 | 1.57e-38 | 138 |
| MS.gene47262.t1 | MTR_4g094982 | 48.062 | 129 | 60 | 1 | 18 | 139 | 4 | 132 | 6.56e-38 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47262.t1 | AT1G69560 | 51.892 | 185 | 76 | 3 | 19 | 193 | 107 | 288 | 4.70e-58 | 188 |
| MS.gene47262.t1 | AT1G69560 | 51.892 | 185 | 76 | 3 | 19 | 193 | 123 | 304 | 6.68e-58 | 188 |
| MS.gene47262.t1 | AT1G26780 | 39.338 | 272 | 128 | 6 | 19 | 265 | 98 | 357 | 1.14e-56 | 186 |
| MS.gene47262.t1 | AT1G17950 | 40.079 | 252 | 135 | 7 | 19 | 267 | 5 | 243 | 2.59e-56 | 181 |
| MS.gene47262.t1 | AT1G26780 | 65.873 | 126 | 42 | 1 | 19 | 144 | 98 | 222 | 2.21e-54 | 177 |
| MS.gene47262.t1 | AT4G33450 | 40.541 | 259 | 129 | 8 | 11 | 265 | 10 | 247 | 1.64e-53 | 174 |
| MS.gene47262.t1 | AT5G17800 | 40.590 | 271 | 107 | 7 | 13 | 265 | 87 | 321 | 2.10e-52 | 173 |
| MS.gene47262.t1 | AT1G73410 | 40.076 | 262 | 118 | 6 | 19 | 267 | 6 | 241 | 1.36e-49 | 164 |
| MS.gene47262.t1 | AT1G73410 | 72.115 | 104 | 29 | 0 | 19 | 122 | 6 | 109 | 3.35e-49 | 161 |
| MS.gene47262.t1 | AT3G29020 | 63.248 | 117 | 43 | 0 | 14 | 130 | 60 | 176 | 4.23e-49 | 162 |
Find 54 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCTGCTCTATTGATTCT+AGG | 0.313747 | 6.3:-18760790 | None:intergenic |
| AAATGTGAGAGATAAGATTA+AGG | 0.412885 | 6.3:+18761758 | MS.gene47262:CDS |
| AACACCAAGCTTTCCATTAG+AGG | 0.318928 | 6.3:+18760158 | MS.gene47262:CDS |
| AACATTGCTGAACAAATACA+TGG | 0.572771 | 6.3:+18760251 | MS.gene47262:CDS |
| AAGCTAACATTTCAAATCCC+AGG | 0.510479 | 6.3:+18760985 | MS.gene47262:CDS |
| AAGGAAGAAAATGAGAAATT+TGG | 0.160898 | 6.3:+18760134 | MS.gene47262:CDS |
| AAGGGAAAAGTTGCAGATTA+AGG | 0.276686 | 6.3:+18760749 | MS.gene47262:intron |
| AAGGTAGAAGTAGGGGACTA+TGG | 0.428911 | 6.3:+18761573 | MS.gene47262:CDS |
| AGAAAGAAAGGTAGAAGTAG+GGG | 0.536632 | 6.3:+18761566 | MS.gene47262:CDS |
| AGAATTTGCAGTGCAATGAG+AGG | 0.669530 | 6.3:+18761603 | MS.gene47262:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATTGCTGAACAAATACA+TGG | + | chr6.3:18760251-18760270 | MS.gene47262:CDS | 30.0% | |
| AGGCTTACTTATAGAAAGAA+AGG | + | chr6.3:18761554-18761573 | MS.gene47262:intron | 30.0% | |
| ATACACTTCATGGTAACAAA+TGG | + | chr6.3:18760848-18760867 | MS.gene47262:CDS | 30.0% | |
| ATAGAAAGAAAGGTAGAAGT+AGG | + | chr6.3:18761564-18761583 | MS.gene47262:CDS | 30.0% | |
| CATACTCATTTGTGAATCAT+AGG | - | chr6.3:18761118-18761137 | None:intergenic | 30.0% | |
| CATTTGATAAGCTAAAGTGA+AGG | - | chr6.3:18761149-18761168 | None:intergenic | 30.0% | |
| GGTTAACATGACTCAAATAT+AGG | + | chr6.3:18761341-18761360 | MS.gene47262:intron | 30.0% | |
| GTATTACATTGGAAATTGTG+CGG | + | chr6.3:18761234-18761253 | MS.gene47262:intron | 30.0% | |
| GTTGAGTTAGATTCATCAAA+CGG | - | chr6.3:18761048-18761067 | None:intergenic | 30.0% | |
| TAGAAAGAAAGGTAGAAGTA+GGG | + | chr6.3:18761565-18761584 | MS.gene47262:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 18760122 | 18761818 | 18760122 | ID=MS.gene47262 |
| chr6.3 | mRNA | 18760122 | 18761818 | 18760122 | ID=MS.gene47262.t1;Parent=MS.gene47262 |
| chr6.3 | exon | 18760122 | 18760281 | 18760122 | ID=MS.gene47262.t1.exon1;Parent=MS.gene47262.t1 |
| chr6.3 | CDS | 18760122 | 18760281 | 18760122 | ID=cds.MS.gene47262.t1;Parent=MS.gene47262.t1 |
| chr6.3 | exon | 18760752 | 18761132 | 18760752 | ID=MS.gene47262.t1.exon2;Parent=MS.gene47262.t1 |
| chr6.3 | CDS | 18760752 | 18761132 | 18760752 | ID=cds.MS.gene47262.t1;Parent=MS.gene47262.t1 |
| chr6.3 | exon | 18761556 | 18761818 | 18761556 | ID=MS.gene47262.t1.exon3;Parent=MS.gene47262.t1 |
| chr6.3 | CDS | 18761556 | 18761818 | 18761556 | ID=cds.MS.gene47262.t1;Parent=MS.gene47262.t1 |
| Gene Sequence |
>MS.gene47262 ATGAAAGTTGACAAGGAAGAAAATGAGAAATTTGGGAACACCAAGCTTTCCATTAGAGGTCATTGGAGACCATCTGAAGATGCTAAGCTTAAAAAACTTGTTGATGAATTTGGTCCTCACAATTGGAACAACATTGCTGAACAAATACATGGAAGATCGGGTATAATCCTAAACTAGAACATAACAAATTTTATTTATTGAAATTTCAAAAAGATTACTAATTTTTTTTTTTTATATCAAAGTTGTCTTAATAATGTAAATAGTTAACTTTCATTTATTTATTTTTTTGACAAGAAGTTTTATTTAAAAAAAAAATTGAATATTTTCAAAAGGTGCTTTTAATATATTTATTAAAATTGCATTAGTATTGTTTTTTGAATGAAATTCTCTTTAATTTGAATTATTTTTTTATATCGATTCTAACGCAATTGGTTGGTGCACAGTGATGATTTTGTTTTTTTTTTTTTTATGACAGTGTTATAATCATTTTTTTAATTTATTTTTTAAACATCTTGATGTTAGATTTAAATTGTGTTTTCAAGATTTATATTTAATTCATTTGTAATATTTTTTGAAGGAGTTTATGTAATGTAACATTTGAGTTTCCTTTTGTGATAATGGTAATTAAAGGGAAAAGTTGCAGATTAAGGTGGTGTAATCAGCTAGATCCTAGAATCAATAGAGCAGCTTTTAGTGAGGAAGAAGAGGAGAAGCTTTTGCATGCTCATACACTTCATGGTAACAAATGGTCACACATTTGCAAATTCTTTCCTGGAAGGACAGACAATGCACTGAAGAATCATTTTCATGTGATCATGGCTAGGAAGCAAAGGGAACTATTATCATCCATTTCAAGAAAGAGAAAGCTAACATTTCAAATCCCAGGAAAGAATAATGTAGCTAGCAAATCAGCCATCACAAATCCGTTTGATGAATCTAACTCAACTTGCACCAATCTCAACCTCACTACTAGGCCTTTGCTTAATTACCAAGCCTATGATTCACAAATGAGTATGCGTTGAATCCTTCACTTTAGCTTATCAAATGATTTTAAATATGAGAAATGCTAACTAGTGTCACAGGGATATTGATTAAGAAAGCCAAATATAGTAGTATTACATTGGAAATTGTGCGGTCAATGCCACAAAAGTGCAAAAAGAGTAATTTTCAACTTAAAACTTCATCTTTTGACTTCTTCAACCAGTGCTCCGGGGACACTGGTTAACATGACTCAAATATAGGTCTGCGGCCACGATATTCAATTGAAGTTGCATCGACCGCATTTAACAGCGATTATAATTTAAAACCTTTAAATTGCTTCAAAATTTTGTTATTTTCTCTCATGGCTTAATATACACTAAATATCCTATATTAATTTATTTGACATATGCTATATATGACCATGTTGTTGATTTTTGTGTTTTATTTTTTTAGGCTTACTTATAGAAAGAAAGGTAGAAGTAGGGGACTATGGTTTTGCCAGAATTTGCAGTGCAATGAGAGGTTGTTCTCAAGGTCACATGGGAAAGCTTAAGAGTGTGGACCAATCCAATCATTCATATTCAAACTCAGAAGTTTCAGTATCTGAGTCAGTTGCAACAAACATGAACAATGAATCAATTTTGGGAGAAAGTGAAAATGTGAGAGATAAGATTAAGGTGTCATTTATTGACTTTCTTGGAGTAGGTGATACATAG |
| Protein sequence |
>MS.gene47262.t1 MKVDKEENEKFGNTKLSIRGHWRPSEDAKLKKLVDEFGPHNWNNIAEQIHGRSGKSCRLRWCNQLDPRINRAAFSEEEEEKLLHAHTLHGNKWSHICKFFPGRTDNALKNHFHVIMARKQRELLSSISRKRKLTFQIPGKNNVASKSAITNPFDESNSTCTNLNLTTRPLLNYQAYDSQMSLLIERKVEVGDYGFARICSAMRGCSQGHMGKLKSVDQSNHSYSNSEVSVSESVATNMNNESILGESENVRDKIKVSFIDFLGVGDT |
