AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47272

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47272.t1 MTR_6g027460 92.167 383 15 3 1 383 1 368 0.0 683
MS.gene47272.t1 MTR_6g027450 73.569 367 59 6 1 367 1 329 0.0 520
MS.gene47272.t1 MTR_7g086780 50.263 380 136 10 15 383 10 347 1.51e-112 335
MS.gene47272.t1 MTR_7g010950 45.876 194 76 6 56 246 94 261 7.57e-43 156
MS.gene47272.t1 MTR_6g012450 32.951 349 165 10 55 383 96 395 4.48e-42 154
MS.gene47272.t1 MTR_4g082060 34.161 322 147 12 9 289 4 301 3.27e-41 151
MS.gene47272.t1 MTR_3g435480 37.736 265 110 10 50 281 98 340 8.21e-41 150
MS.gene47272.t1 MTR_8g044220 33.459 266 114 8 41 289 67 286 8.55e-37 139
MS.gene47272.t1 MTR_5g041420 53.509 114 44 2 55 162 99 209 3.42e-34 129
MS.gene47272.t1 MTR_5g041530 52.294 109 49 1 54 162 100 205 2.09e-30 120
MS.gene47272.t1 MTR_2g016030 77.419 62 14 0 89 150 39 100 4.16e-30 114
MS.gene47272.t1 MTR_5g041380 62.791 86 27 2 65 150 100 180 2.44e-29 117
MS.gene47272.t1 MTR_5g041400 57.778 90 32 2 61 150 89 172 1.03e-28 115
MS.gene47272.t1 MTR_8g068210 47.872 94 36 2 59 152 7 87 4.62e-24 102
MS.gene47272.t1 MTR_8g027295 60.317 63 25 0 92 154 19 81 1.34e-23 99.4
MS.gene47272.t1 MTR_4g088580 44.762 105 48 2 56 152 8 110 2.33e-23 100
MS.gene47272.t1 MTR_4g022370 60.656 61 24 0 90 150 75 135 2.35e-23 100
MS.gene47272.t1 MTR_4g461080 55.844 77 33 1 74 149 5 81 2.71e-23 98.6
MS.gene47272.t1 MTR_2g093220 55.263 76 33 1 76 150 18 93 5.25e-23 98.2
MS.gene47272.t1 MTR_3g091820 54.930 71 32 0 81 151 27 97 5.72e-23 98.2
MS.gene47272.t1 MTR_8g479350 52.703 74 35 0 97 170 68 141 9.74e-23 98.2
MS.gene47272.t1 MTR_2g096740 56.716 67 29 0 84 150 64 130 1.46e-22 97.1
MS.gene47272.t1 MTR_2g096740 56.716 67 29 0 84 150 49 115 1.52e-22 96.7
MS.gene47272.t1 MTR_4g063780 61.667 60 23 0 90 149 68 127 2.14e-22 97.1
MS.gene47272.t1 MTR_8g079060 42.623 122 63 3 97 218 29 143 2.62e-22 94.7
MS.gene47272.t1 MTR_4g089095 48.485 99 35 2 69 152 7 104 8.65e-22 94.7
MS.gene47272.t1 MTR_2g013370 67.241 58 18 1 95 152 44 100 1.53e-21 93.6
MS.gene47272.t1 MTR_2g059540 69.388 49 15 0 97 145 31 79 1.98e-21 91.7
MS.gene47272.t1 MTR_4g089095 69.643 56 16 1 97 152 16 70 2.56e-21 92.8
MS.gene47272.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.59e-21 92.8
MS.gene47272.t1 MTR_2g014060 54.688 64 29 0 85 148 13 76 2.63e-21 94.0
MS.gene47272.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.89e-21 93.2
MS.gene47272.t1 MTR_7g059400 53.521 71 31 1 82 150 59 129 2.96e-21 94.0
MS.gene47272.t1 MTR_8g015840 65.385 52 18 0 97 148 20 71 3.57e-21 91.3
MS.gene47272.t1 MTR_4g109980 64.151 53 19 0 95 147 38 90 5.97e-21 92.8
MS.gene47272.t1 MTR_7g024670 67.308 52 17 0 98 149 78 129 7.31e-21 93.2
MS.gene47272.t1 MTR_3g090430 54.167 72 31 1 81 150 33 104 2.97e-20 90.9
MS.gene47272.t1 MTR_5g031440 60.000 55 22 0 93 147 39 93 3.40e-20 90.9
MS.gene47272.t1 MTR_2g014170 67.347 49 16 0 97 145 45 93 8.22e-20 89.4
MS.gene47272.t1 MTR_3g077750 56.452 62 26 1 87 147 50 111 1.39e-19 89.4
MS.gene47272.t1 MTR_1g056810 65.385 52 18 0 99 150 14 65 1.66e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47272.t1 AT3G47500 56.667 120 46 3 53 172 71 184 2.95e-39 145
MS.gene47272.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.35e-36 136
MS.gene47272.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.35e-36 136
MS.gene47272.t1 AT5G39660 44.828 174 61 6 63 221 92 245 9.35e-36 136
MS.gene47272.t1 AT5G62430 52.137 117 49 2 64 178 26 137 6.64e-35 130
MS.gene47272.t1 AT2G34140 58.065 93 26 1 71 150 19 111 9.86e-34 124
MS.gene47272.t1 AT1G26790 48.951 143 60 4 55 184 67 209 1.12e-33 129
MS.gene47272.t1 AT1G69570 45.385 130 43 2 85 213 120 222 3.38e-31 122
MS.gene47272.t1 AT1G29160 78.333 60 13 0 91 150 56 115 3.80e-31 117
MS.gene47272.t1 AT3G55370 57.143 70 30 0 93 162 72 141 1.91e-24 102
MS.gene47272.t1 AT3G55370 57.143 70 30 0 93 162 72 141 2.19e-24 103
MS.gene47272.t1 AT3G55370 57.143 70 30 0 93 162 117 186 4.62e-24 102
MS.gene47272.t1 AT2G28810 40.000 100 57 1 52 148 29 128 5.04e-23 99.0
MS.gene47272.t1 AT2G28810 40.000 100 57 1 52 148 46 145 5.77e-23 99.0
MS.gene47272.t1 AT5G60200 59.375 64 26 0 91 154 49 112 7.52e-23 97.1
MS.gene47272.t1 AT3G21270 69.811 53 16 0 97 149 29 81 1.10e-22 95.1
MS.gene47272.t1 AT2G37590 62.069 58 22 0 93 150 85 142 1.76e-22 97.4
MS.gene47272.t1 AT3G45610 45.055 91 42 2 93 175 36 126 2.34e-22 95.1
MS.gene47272.t1 AT1G51700 52.857 70 31 1 79 148 17 84 4.09e-22 93.2
MS.gene47272.t1 AT1G28310 64.286 56 20 0 97 152 27 82 1.32e-21 94.7
MS.gene47272.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.33e-21 94.0
MS.gene47272.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.33e-21 94.0
MS.gene47272.t1 AT4G24060 56.923 65 28 0 86 150 42 106 1.45e-21 95.1
MS.gene47272.t1 AT1G28310 64.286 56 20 0 97 152 51 106 1.46e-21 94.7
MS.gene47272.t1 AT3G61850 61.017 59 23 0 92 150 69 127 1.80e-21 94.0
MS.gene47272.t1 AT5G60850 66.667 54 18 0 97 150 51 104 1.91e-21 94.0
MS.gene47272.t1 AT3G61850 61.017 59 23 0 92 150 57 115 2.43e-21 94.0
MS.gene47272.t1 AT1G07640 66.038 53 18 0 97 149 21 73 2.52e-21 93.2
MS.gene47272.t1 AT3G50410 66.038 53 18 0 97 149 30 82 2.73e-21 92.4
MS.gene47272.t1 AT2G28510 66.071 56 19 0 99 154 50 105 2.80e-21 93.2
MS.gene47272.t1 AT3G61850 61.017 59 23 0 92 150 69 127 2.85e-21 94.0
MS.gene47272.t1 AT1G07640 66.038 53 18 0 97 149 77 129 3.81e-21 93.6
MS.gene47272.t1 AT5G02460 59.016 61 25 0 90 150 88 148 4.45e-21 94.4
MS.gene47272.t1 AT5G66940 53.425 73 27 1 84 156 26 91 4.65e-21 91.3
MS.gene47272.t1 AT1G07640 66.038 53 18 0 97 149 85 137 5.17e-21 93.2
MS.gene47272.t1 AT5G65590 64.815 54 19 0 97 150 41 94 6.33e-21 92.8
MS.gene47272.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.93e-20 90.9
MS.gene47272.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.93e-20 90.9
MS.gene47272.t1 AT4G00940 52.778 72 30 1 85 152 52 123 1.93e-20 90.9
MS.gene47272.t1 AT1G21340 42.553 94 53 1 99 191 40 133 4.89e-20 89.4
MS.gene47272.t1 AT5G62940 39.450 109 50 2 55 147 15 123 7.12e-20 90.5
MS.gene47272.t1 AT4G21050 47.436 78 39 1 77 152 2 79 7.91e-20 87.4
MS.gene47272.t1 AT4G38000 49.367 79 38 2 96 172 40 118 1.00e-19 87.8
MS.gene47272.t1 AT3G52440 63.462 52 19 0 99 150 27 78 2.11e-19 87.0
MS.gene47272.t1 AT1G64620 53.333 60 28 0 86 145 38 97 2.50e-19 88.6
MS.gene47272.t1 AT1G47655 65.306 49 17 0 97 145 29 77 3.37e-19 85.5
MS.gene47272.t1 AT3G52440 63.462 52 19 0 99 150 46 97 3.39e-19 87.0
MS.gene47272.t1 AT2G46590 58.491 53 22 0 92 144 63 115 1.30e-18 86.7
MS.gene47272.t1 AT2G46590 58.491 53 22 0 92 144 75 127 1.54e-18 86.7
MS.gene47272.t1 AT4G21040 48.611 72 37 0 99 170 27 98 9.35e-16 76.3
MS.gene47272.t1 AT4G21080 50.877 57 28 0 99 155 27 83 1.09e-14 73.6

Find 82 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTATTGTCCTAAAATT+TGG 0.197738 6.3:-18972988 MS.gene47272:CDS
CAATCTTGTCTGGTTTCTTT+AGG 0.212759 6.3:+18973310 None:intergenic
TAGAAACCAACGACTCTTCT+AGG 0.239536 6.3:-18973065 MS.gene47272:CDS
TTGGAACAATGTTACTTCTA+TGG 0.251370 6.3:-18972722 MS.gene47272:CDS
GGTAACAATTGTAGAGAAAA+TGG 0.277928 6.3:-18972886 MS.gene47272:CDS
GGTGAGTTCTTCTCAGATTC+AGG 0.283209 6.3:-18974450 MS.gene47272:CDS
AAGATTAAGCATTGAACCTT+TGG 0.298547 6.3:+18972939 None:intergenic
TCTTCTAGTTTCATGCTCTT+TGG 0.299226 6.3:-18974485 MS.gene47272:CDS
TTTAGGTGTTCAAATGATAA+TGG 0.318424 6.3:-18973006 MS.gene47272:CDS
GTCGGAGTTGTCACAGATAT+TGG 0.320960 6.3:-18973206 MS.gene47272:CDS
TTGTTCCAACTTTGATTCAT+TGG 0.340788 6.3:+18972736 None:intergenic
ATTTACCATGGTTGTAGAAT+TGG 0.341653 6.3:+18974427 None:intergenic
AAGCACTGTGGAAGATAATA+TGG 0.348391 6.3:-18973361 MS.gene47272:CDS
CCTTTGTATGGTAGTGGAAA+TGG 0.370217 6.3:-18972553 MS.gene47272:CDS
ATGCAGATGTGTAACCCTTA+TGG 0.380909 6.3:-18972688 MS.gene47272:CDS
AGAGTCGTTGGTTTCTAAAC+TGG 0.381811 6.3:+18973071 None:intergenic
GGACATTGAACAATCTTGTC+TGG 0.411323 6.3:+18973300 None:intergenic
ACAAATTGCAAGCCAATGTT+TGG 0.418330 6.3:+18972601 None:intergenic
GCTCTTTGGTAAGAAGATTC+CGG 0.421687 6.3:-18974471 MS.gene47272:CDS
TGCAGTAACTCAAAGCATAA+AGG 0.422749 6.3:-18973462 MS.gene47272:CDS
AGGTTTAGACCTATTCTGTT+CGG 0.445629 6.3:+18972792 None:intergenic
AATGCAACCATTTGAACCTA+TGG 0.446070 6.3:+18972522 None:intergenic
GTGTCACGCGCCGAGGGTTG+CGG 0.447216 6.3:-18972641 MS.gene47272:CDS
AGAGCTACCCTGCACCTTCA+TGG 0.447832 6.3:-18972765 MS.gene47272:CDS
ACTAGTAATAGTTGTATCTC+AGG 0.449615 6.3:-18972481 MS.gene47272:CDS
AATTTGTTCCTGCATCAAAC+AGG 0.461385 6.3:-18972585 MS.gene47272:CDS
AGAAATGTTGCTGTCGGTGC+AGG 0.467099 6.3:-18973165 MS.gene47272:CDS
GTTCCATTCCTGTTTGATGC+AGG 0.467432 6.3:+18972577 None:intergenic
TTCATTGGAATTATCCATGA+AGG 0.472286 6.3:+18972751 None:intergenic
TTGCATCAACATTTCTCTTG+TGG 0.474797 6.3:+18972911 None:intergenic
TATTGTCCTAAAATTTGGTC+GGG 0.484036 6.3:-18972983 MS.gene47272:CDS
TATTCGAATCCGAACAGAAT+AGG 0.487381 6.3:-18972801 MS.gene47272:CDS
GAAGTAGGATCAATGCCATA+AGG 0.489322 6.3:+18972673 None:intergenic
GGAATTATCCATGAAGGTGC+AGG 0.491213 6.3:+18972757 None:intergenic
CATGTGTGTTTGCCAAGGAT+TGG 0.493826 6.3:+18972448 None:intergenic
AATAGTTGTATCTCAGGTAA+TGG 0.494093 6.3:-18972475 MS.gene47272:CDS
GGAACACCTTTGTATGGTAG+TGG 0.497692 6.3:-18972559 MS.gene47272:CDS
TTGCTGTCGGTGCAGGGAGA+CGG 0.499466 6.3:-18973158 MS.gene47272:CDS
TAATTCCAATGAATCAAAGT+TGG 0.500347 6.3:-18972741 MS.gene47272:CDS
TGCAGTGGTGTCACGCGCCG+AGG 0.505833 6.3:-18972648 MS.gene47272:CDS
ATTTCTCATTGTTCCACCGT+TGG 0.510921 6.3:+18973182 None:intergenic
GCAGTGGTGTCACGCGCCGA+GGG 0.511659 6.3:-18972647 MS.gene47272:CDS
GGTAATGGCACACCAATCCT+TGG 0.524374 6.3:-18972460 MS.gene47272:CDS
AGGAATGGAACACCTTTGTA+TGG 0.533609 6.3:-18972565 MS.gene47272:CDS
TTGGTACCTAGAAGAGTCGT+TGG 0.535519 6.3:+18973059 None:intergenic
GTATTGTCCTAAAATTTGGT+CGG 0.547769 6.3:-18972984 MS.gene47272:CDS
ATTCAACATTTGAGCCATGT+TGG 0.548791 6.3:+18973040 None:intergenic
AATGTGTCCATAGGTTCAAA+TGG 0.549266 6.3:-18972529 MS.gene47272:CDS
GTTCCTGCATCAAACAGGAA+TGG 0.554691 6.3:-18972580 MS.gene47272:CDS
AAGAGAAATGTTGATGCAAG+TGG 0.555436 6.3:-18972907 MS.gene47272:CDS
TGTGTATCGTCTGTGACAAA+CGG 0.557160 6.3:-18972844 MS.gene47272:CDS
ACCTTTGGATTCATCGAGAG+AGG 0.559535 6.3:+18972954 None:intergenic
AAGTAGGATCAATGCCATAA+GGG 0.561604 6.3:+18972674 None:intergenic
GGAAATGGAAATGTGTCCAT+AGG 0.578755 6.3:-18972538 MS.gene47272:CDS
TTGGTTTCTAAACTGGCGGT+CGG 0.586129 6.3:+18973078 None:intergenic
AATCTCATGTGTGTTTGCCA+AGG 0.586652 6.3:+18972443 None:intergenic
TGACACCACTGCATTGAAGT+AGG 0.587068 6.3:+18972658 None:intergenic
ATCAACCTCGGCATTTCTGT+CGG 0.588083 6.3:-18973224 MS.gene47272:CDS
ATGTTTGTAACCGCAACCCT+CGG 0.590311 6.3:+18972631 None:intergenic
TCAGGCCAATTCTACAACCA+TGG 0.595350 6.3:-18974432 MS.gene47272:intron
GGTGCAGGGTAGCTCTGCAT+AGG 0.600830 6.3:+18972772 None:intergenic
CACATGATTGTAGTGTCCGA+TGG 0.602575 6.3:-18973105 MS.gene47272:CDS
GTCGTTGGTTTCTAAACTGG+CGG 0.606694 6.3:+18973074 None:intergenic
CCATTTCCACTACCATACAA+AGG 0.611279 6.3:+18972553 None:intergenic
CTCAAATGTTGAATCTGCTG+CGG 0.611992 6.3:-18973031 MS.gene47272:CDS
GCCTCTCTCGATGAATCCAA+AGG 0.612065 6.3:-18972955 MS.gene47272:CDS
TTACTAGTAGTAGATGAAGA+AGG 0.612171 6.3:+18972496 None:intergenic
CACAGATATTGGACCAACGG+TGG 0.629754 6.3:-18973195 MS.gene47272:CDS
TGAATCTGAGAAGAACTCAC+CGG 0.630634 6.3:+18974452 None:intergenic
GAATTATCCATGAAGGTGCA+GGG 0.632904 6.3:+18972758 None:intergenic
TGTCACAGATATTGGACCAA+CGG 0.638912 6.3:-18973198 MS.gene47272:CDS
ACTGGCGGTCGGAATTCCAT+CGG 0.649455 6.3:+18973089 None:intergenic
TTGATCCTACTTCAATGCAG+TGG 0.649462 6.3:-18972663 MS.gene47272:CDS
GTGACAAACGGTCATACTCG+AGG 0.662893 6.3:-18972832 MS.gene47272:CDS
GAAATGTTGCTGTCGGTGCA+GGG 0.663844 6.3:-18973164 MS.gene47272:CDS
CAACTCCGACAGAAATGCCG+AGG 0.680790 6.3:+18973219 None:intergenic
ACTACAACGTCAATCAACCT+CGG 0.682406 6.3:-18973236 MS.gene47272:CDS
GACTCTTCTAGGTACCAACA+TGG 0.687547 6.3:-18973054 MS.gene47272:CDS
ACAATGAGAAATGTTGCTGT+CGG 0.691886 6.3:-18973171 MS.gene47272:CDS
CATCGGACACTACAATCATG+TGG 0.695881 6.3:+18973106 None:intergenic
CAATTGTAGAGAAAATGGAG+AGG 0.705347 6.3:-18972881 MS.gene47272:CDS
AAAAGTGAACACAAGCACTG+TGG 0.727270 6.3:-18973373 MS.gene47272:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTTGTTGTAT+TGG - chr6.3:18972636-18972655 MS.gene47272:CDS 10.0%
!!! TTTTTTATTTTTGTTGTATT+GGG - chr6.3:18972637-18972656 MS.gene47272:CDS 10.0%
!! AATTTAAATGTTTCAAAGAT+TGG + chr6.3:18972678-18972697 None:intergenic 15.0%
!! ATAAAAAAAACTCAAGAATA+TGG - chr6.3:18972741-18972760 MS.gene47272:CDS 15.0%
!! TAAAAAAACAACATAAACAA+TGG + chr6.3:18972599-18972618 None:intergenic 15.0%
!! TTTCATAAATATAACATGAT+TGG - chr6.3:18973117-18973136 MS.gene47272:CDS 15.0%
!!! TAAATTTGTTTAATTTTGCT+TGG - chr6.3:18973037-18973056 MS.gene47272:CDS 15.0%
!! AATTTAAGAAACTGAGTTTA+AGG - chr6.3:18972889-18972908 MS.gene47272:CDS 20.0%
!!! TTAAGAGTATTTGATCTATA+TGG - chr6.3:18972699-18972718 MS.gene47272:CDS 20.0%
! AAGTCATGAAGAATAAAGTT+TGG + chr6.3:18973447-18973466 None:intergenic 25.0%
! AATGGTATTGTCCTAAAATT+TGG - chr6.3:18973977-18973996 MS.gene47272:intron 25.0%
! ACAACATAAACAATGGTTAT+TGG + chr6.3:18972592-18972611 None:intergenic 25.0%
! CAACATAAACAATGGTTATT+GGG + chr6.3:18972591-18972610 None:intergenic 25.0%
! GATAAAATGAGTATTTACCA+TGG + chr6.3:18972553-18972572 None:intergenic 25.0%
! TAATTCCAATGAATCAAAGT+TGG - chr6.3:18974224-18974243 MS.gene47272:intron 25.0%
! TTTAGGTGTTCAAATGATAA+TGG - chr6.3:18973959-18973978 MS.gene47272:intron 25.0%
!!! TTATTTTTGTTGTATTGGGT+TGG - chr6.3:18972641-18972660 MS.gene47272:CDS 25.0%
AAAAACCATAAAATTCGACG+TGG + chr6.3:18973366-18973385 None:intergenic 30.0%
AATAGTTGTATCTCAGGTAA+TGG - chr6.3:18974490-18974509 MS.gene47272:CDS 30.0%
ACTAGTAATAGTTGTATCTC+AGG - chr6.3:18974484-18974503 MS.gene47272:CDS 30.0%
ATTTACCATGGTTGTAGAAT+TGG + chr6.3:18972541-18972560 None:intergenic 30.0%
GATCTGATTCATGTAAACAA+TGG - chr6.3:18972763-18972782 MS.gene47272:CDS 30.0%
GGTAACAATTGTAGAGAAAA+TGG - chr6.3:18974079-18974098 MS.gene47272:intron 30.0%
GTATTGTCCTAAAATTTGGT+CGG - chr6.3:18973981-18974000 MS.gene47272:intron 30.0%
TAGTGAGTTTGACAAAATCA+CGG - chr6.3:18973226-18973245 MS.gene47272:CDS 30.0%
TATTGTCCTAAAATTTGGTC+GGG - chr6.3:18973982-18974001 MS.gene47272:intron 30.0%
TGTGAAATAGACTGAAATTG+TGG - chr6.3:18973149-18973168 MS.gene47272:CDS 30.0%
TTACTAGTAGTAGATGAAGA+AGG + chr6.3:18974472-18974491 None:intergenic 30.0%
TTCATTGGAATTATCCATGA+AGG + chr6.3:18974217-18974236 None:intergenic 30.0%
TTGTTCCAACTTTGATTCAT+TGG + chr6.3:18974232-18974251 None:intergenic 30.0%
! AACATTTTCACACCAAACAT+TGG - chr6.3:18974352-18974371 MS.gene47272:intron 30.0%
! GATTTTGTCAAACTCACTAT+GGG + chr6.3:18973225-18973244 None:intergenic 30.0%
! TGATTTTGTCAAACTCACTA+TGG + chr6.3:18973226-18973245 None:intergenic 30.0%
! TTGGAACAATGTTACTTCTA+TGG - chr6.3:18974243-18974262 MS.gene47272:intron 30.0%
!! AAGATTAAGCATTGAACCTT+TGG + chr6.3:18974029-18974048 None:intergenic 30.0%
!! TTCTCAATGTTTTGAGTATG+AGG + chr6.3:18973539-18973558 None:intergenic 30.0%
AAGAGAAATGTTGATGCAAG+TGG - chr6.3:18974058-18974077 MS.gene47272:intron 35.0%
AAGTAGGATCAATGCCATAA+GGG + chr6.3:18974294-18974313 None:intergenic 35.0%
AATGCAACCATTTGAACCTA+TGG + chr6.3:18974446-18974465 None:intergenic 35.0%
AATGTGTCCATAGGTTCAAA+TGG - chr6.3:18974436-18974455 MS.gene47272:CDS 35.0%
ACAAATTGCAAGCCAATGTT+TGG + chr6.3:18974367-18974386 None:intergenic 35.0%
ACAATGAGAAATGTTGCTGT+CGG - chr6.3:18973794-18973813 MS.gene47272:intron 35.0%
AGCTGTCTTATGAAAGTAGT+TGG - chr6.3:18972919-18972938 MS.gene47272:CDS 35.0%
AGGTTTAGACCTATTCTGTT+CGG + chr6.3:18974176-18974195 None:intergenic 35.0%
ATTCAACATTTGAGCCATGT+TGG + chr6.3:18973928-18973947 None:intergenic 35.0%
CAATTGTAGAGAAAATGGAG+AGG - chr6.3:18974084-18974103 MS.gene47272:intron 35.0%
TATGGGTAATTCCATAAACC+AGG + chr6.3:18973208-18973227 None:intergenic 35.0%
TATTCGAATCCGAACAGAAT+AGG - chr6.3:18974164-18974183 MS.gene47272:intron 35.0%
TGATTCATGTAAACAATGGC+TGG - chr6.3:18972767-18972786 MS.gene47272:CDS 35.0%
TGCAGTAACTCAAAGCATAA+AGG - chr6.3:18973503-18973522 MS.gene47272:intron 35.0%
TTGCATCAACATTTCTCTTG+TGG + chr6.3:18974057-18974076 None:intergenic 35.0%
! AAGCACTGTGGAAGATAATA+TGG - chr6.3:18973604-18973623 MS.gene47272:intron 35.0%
! AATTTGTTCCTGCATCAAAC+AGG - chr6.3:18974380-18974399 MS.gene47272:intron 35.0%
! ATGTACCACGTCGAATTTTA+TGG - chr6.3:18973358-18973377 MS.gene47272:CDS 35.0%
! CAATCTTGTCTGGTTTCTTT+AGG + chr6.3:18973658-18973677 None:intergenic 35.0%
! CATTTTCCCGACCAAATTTT+AGG + chr6.3:18973991-18974010 None:intergenic 35.0%
!! TCTTCTAGTTTCATGCTCTT+TGG - chr6.3:18972480-18972499 MS.gene47272:CDS 35.0%
ACTACAACGTCAATCAACCT+CGG - chr6.3:18973729-18973748 MS.gene47272:intron 40.0%
AGGAATGGAACACCTTTGTA+TGG - chr6.3:18974400-18974419 MS.gene47272:intron 40.0%
ATGCAGATGTGTAACCCTTA+TGG - chr6.3:18974277-18974296 MS.gene47272:intron 40.0%
ATTTCTCATTGTTCCACCGT+TGG + chr6.3:18973786-18973805 None:intergenic 40.0%
CCATTTCCACTACCATACAA+AGG + chr6.3:18974415-18974434 None:intergenic 40.0%
CTCAAATGTTGAATCTGCTG+CGG - chr6.3:18973934-18973953 MS.gene47272:intron 40.0%
GAAGTAGGATCAATGCCATA+AGG + chr6.3:18974295-18974314 None:intergenic 40.0%
GCTCTTTGGTAAGAAGATTC+CGG - chr6.3:18972494-18972513 MS.gene47272:CDS 40.0%
GGAAATGGAAATGTGTCCAT+AGG - chr6.3:18974427-18974446 MS.gene47272:intron 40.0%
GGACATTGAACAATCTTGTC+TGG + chr6.3:18973668-18973687 None:intergenic 40.0%
GGTAATTCCATAAACCAGGA+GGG + chr6.3:18973204-18973223 None:intergenic 40.0%
TAGAAACCAACGACTCTTCT+AGG - chr6.3:18973900-18973919 MS.gene47272:intron 40.0%
TGAATCTGAGAAGAACTCAC+CGG + chr6.3:18972516-18972535 None:intergenic 40.0%
TGTCACAGATATTGGACCAA+CGG - chr6.3:18973767-18973786 MS.gene47272:intron 40.0%
TGTGTATCGTCTGTGACAAA+CGG - chr6.3:18974121-18974140 MS.gene47272:intron 40.0%
TTGATCCTACTTCAATGCAG+TGG - chr6.3:18974302-18974321 MS.gene47272:intron 40.0%
! AAAAGTGAACACAAGCACTG+TGG - chr6.3:18973592-18973611 MS.gene47272:intron 40.0%
! GAATTATCCATGAAGGTGCA+GGG + chr6.3:18974210-18974229 None:intergenic 40.0%
! GTGTCGAAACTTTTGCATCT+CGG + chr6.3:18973689-18973708 None:intergenic 40.0%
! TTGAATCTGCTGCGGTTTTT+AGG - chr6.3:18973942-18973961 MS.gene47272:intron 40.0%
!! AGAGTCGTTGGTTTCTAAAC+TGG + chr6.3:18973897-18973916 None:intergenic 40.0%
!! CCTTTGTATGGTAGTGGAAA+TGG - chr6.3:18974412-18974431 MS.gene47272:intron 40.0%
AACATAAGAGATAGCCCTCC+TGG - chr6.3:18973187-18973206 MS.gene47272:CDS 45.0%
ACCTTTGGATTCATCGAGAG+AGG + chr6.3:18974014-18974033 None:intergenic 45.0%
ATCAACCTCGGCATTTCTGT+CGG - chr6.3:18973741-18973760 MS.gene47272:intron 45.0%
ATGTTTGTAACCGCAACCCT+CGG + chr6.3:18974337-18974356 None:intergenic 45.0%
CACATGATTGTAGTGTCCGA+TGG - chr6.3:18973860-18973879 MS.gene47272:intron 45.0%
CATCGGACACTACAATCATG+TGG + chr6.3:18973862-18973881 None:intergenic 45.0%
CATGTGTGTTTGCCAAGGAT+TGG + chr6.3:18974520-18974539 None:intergenic 45.0%
GACTCTTCTAGGTACCAACA+TGG - chr6.3:18973911-18973930 MS.gene47272:intron 45.0%
GGAACACCTTTGTATGGTAG+TGG - chr6.3:18974406-18974425 MS.gene47272:intron 45.0%
GGGTAATTCCATAAACCAGG+AGG + chr6.3:18973205-18973224 None:intergenic 45.0%
GGTGAGTTCTTCTCAGATTC+AGG - chr6.3:18972515-18972534 MS.gene47272:CDS 45.0%
GTCGGAGTTGTCACAGATAT+TGG - chr6.3:18973759-18973778 MS.gene47272:intron 45.0%
GTTCCATTCCTGTTTGATGC+AGG + chr6.3:18974391-18974410 None:intergenic 45.0%
GTTCCTGCATCAAACAGGAA+TGG - chr6.3:18974385-18974404 MS.gene47272:intron 45.0%
TCAGGCCAATTCTACAACCA+TGG - chr6.3:18972533-18972552 MS.gene47272:CDS 45.0%
TGACACCACTGCATTGAAGT+AGG + chr6.3:18974310-18974329 None:intergenic 45.0%
TTGGTACCTAGAAGAGTCGT+TGG + chr6.3:18973909-18973928 None:intergenic 45.0%
TTGGTTTCTAAACTGGCGGT+CGG + chr6.3:18973890-18973909 None:intergenic 45.0%
! GGAATTATCCATGAAGGTGC+AGG + chr6.3:18974211-18974230 None:intergenic 45.0%
!! GTCGTTGGTTTCTAAACTGG+CGG + chr6.3:18973894-18973913 None:intergenic 45.0%
!!! TAAAAAAATGTTTTTTTTTT+TGG - chr6.3:18972811-18972830 MS.gene47272:CDS 5.0%
AGAAATGTTGCTGTCGGTGC+AGG - chr6.3:18973800-18973819 MS.gene47272:intron 50.0%
CACAGATATTGGACCAACGG+TGG - chr6.3:18973770-18973789 MS.gene47272:intron 50.0%
GAAATGTTGCTGTCGGTGCA+GGG - chr6.3:18973801-18973820 MS.gene47272:intron 50.0%
GAGATAGCCCTCCTGGTTTA+TGG - chr6.3:18973194-18973213 MS.gene47272:CDS 50.0%
GCCTCTCTCGATGAATCCAA+AGG - chr6.3:18974010-18974029 MS.gene47272:intron 50.0%
GTGACAAACGGTCATACTCG+AGG - chr6.3:18974133-18974152 MS.gene47272:intron 50.0%
! GGTAATGGCACACCAATCCT+TGG - chr6.3:18974505-18974524 MS.gene47272:CDS 50.0%
ACTGGCGGTCGGAATTCCAT+CGG + chr6.3:18973879-18973898 None:intergenic 55.0%
AGAGCTACCCTGCACCTTCA+TGG - chr6.3:18974200-18974219 MS.gene47272:intron 55.0%
CAACTCCGACAGAAATGCCG+AGG + chr6.3:18973749-18973768 None:intergenic 55.0%
TTGCTGTCGGTGCAGGGAGA+CGG - chr6.3:18973807-18973826 MS.gene47272:intron 60.0%
! GGTGCAGGGTAGCTCTGCAT+AGG + chr6.3:18974196-18974215 None:intergenic 60.0%
GTGTCACGCGCCGAGGGTTG+CGG - chr6.3:18974324-18974343 MS.gene47272:intron 70.0%
!! GCAGTGGTGTCACGCGCCGA+GGG - chr6.3:18974318-18974337 MS.gene47272:intron 70.0%
!! TGCAGTGGTGTCACGCGCCG+AGG - chr6.3:18974317-18974336 MS.gene47272:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 18972447 18974540 18972447 ID=MS.gene47272
chr6.3 mRNA 18972447 18974540 18972447 ID=MS.gene47272.t1;Parent=MS.gene47272
chr6.3 exon 18974433 18974540 18974433 ID=MS.gene47272.t1.exon1;Parent=MS.gene47272.t1
chr6.3 CDS 18974433 18974540 18974433 ID=cds.MS.gene47272.t1;Parent=MS.gene47272.t1
chr6.3 exon 18972447 18973490 18972447 ID=MS.gene47272.t1.exon2;Parent=MS.gene47272.t1
chr6.3 CDS 18972447 18973490 18972447 ID=cds.MS.gene47272.t1;Parent=MS.gene47272.t1
Gene Sequence

>MS.gene47272

ATGAATGGTGAAGGTGACATGTTTGTTAAAGATTCTTCTAGTTTCATGCTCTTTGGTAAGAAGATTCCGGTGAGTTCTTCTCAGATTCAGGCCAATTCTACAACCATGGTAAATACTCATTTTATCATATTATATCATATACCCAATAACCATTGTTTATGTTGTTTTTTTATTTGAAAAAATTTAAGTATTTTTTATTTTTGTTGTATTGGGTTGGTATAATATTTTCCAATCTTTGAAACATTTAAATTTTTAAGAGTATTTGATCTATATGGTTTCTTTTTTAATATATTGATAAAAAAAACTCAAGAATATGGATCTGATTCATGTAAACAATGGCTGGTCTTAATTGTATGAATTCTAATAAAAAAATGTTTTTTTTTTTGGAAATTTATAGTTGAGAATATATGTTTATGTCAATACATACATACATATATAGATGAATTTAAGAAACTGAGTTTAAGGTTTGTTAAGCTGTCTTATGAAAGTAGTTGGTTTTTTAAATAAAATTAGATCTGTTGCATTTTTTATGATACACTTATATTATTTTTGTCTGAAATTGAGTTGTACTTTTGAAAGAAATTTAACTTTAAATTTGTTTAATTTTGCTTGGAGTATTAATCAAATCTTAAAAACGTTCAATGTTTAATCAATTACAAATAGATTTGTTTTTCATAAATATAACATGATTGGTTGAATGAATGTGAAATAGACTGAAATTGTGGCAGATTAGTTTTAGTAACATAAGAGATAGCCCTCCTGGTTTATGGAATTACCCATAGTGAGTTTGACAAAATCACGGTGACATGTTTGTTGAAGCTCGAAAGAGTAAATTTTTCTAAATTACAGTGACACGTGATTATGTCAAACTCATCGTCAATCTAAACATACGAAATGAAAGTTCTCATGTTATGTACCACGTCGAATTTTATGGTTTTTTTATGTGAAAAATGAATATAACATTGATTTTGTTTCTTTTTTATTGTCAAATTTGTTGCCAAACTTTATTCTTCATGACTTATCACTGATTATATAGTTTTTATTTTACAGATTTCTTGCAGTAACTCAAAGCATAAAGGAAATGATATGCCTCATACTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTGAACACAAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTAAAGAAACCAGACAAGATTGTTCAATGTCCGAGATGCAAAAGTTTCGACACAAAATTTTGTTATTTCAACAACTACAACGTCAATCAACCTCGGCATTTCTGTCGGAGTTGTCACAGATATTGGACCAACGGTGGAACAATGAGAAATGTTGCTGTCGGTGCAGGGAGACGGAAGAACAAACACATAGCTTCTCAATATCGCCACATGATTGTAGTGTCCGATGGAATTCCGACCGCCAGTTTAGAAACCAACGACTCTTCTAGGTACCAACATGGCTCAAATGTTGAATCTGCTGCGGTTTTTAGGTGTTCAAATGATAATGGTATTGTCCTAAAATTTGGTCGGGAAAATGCCTCTCTCGATGAATCCAAAGGTTCAATGCTTAATCTTATGAACCACAAGAGAAATGTTGATGCAAGTGGTAACAATTGTAGAGAAAATGGAGAGGAAGAATCATCGTTATGTGTATCGTCTGTGACAAACGGTCATACTCGAGGAAACGAGTTATTCGAATCCGAACAGAATAGGTCTAAACCTATGCAGAGCTACCCTGCACCTTCATGGATAATTCCAATGAATCAAAGTTGGAACAATGTTACTTCTATGGTTCAATCATCAATGCAGATGTGTAACCCTTATGGCATTGATCCTACTTCAATGCAGTGGTGTCACGCGCCGAGGGTTGCGGTTACAAACATTTTCACACCAAACATTGGCTTGCAATTTGTTCCTGCATCAAACAGGAATGGAACACCTTTGTATGGTAGTGGAAATGGAAATGTGTCCATAGGTTCAAATGGTTGCATTTCACCTTCTTCATCTACTACTAGTAATAGTTGTATCTCAGGTAATGGCACACCAATCCTTGGCAAACACACATGA
Protein sequence

>MS.gene47272.t1

MNGEGDMFVKDSSSFMLFGKKIPVSSSQIQANSTTMISCSNSKHKGNDMPHTQNIEKSKNSSNEENESKVNTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCKSFDTKFCYFNNYNVNQPRHFCRSCHRYWTNGGTMRNVAVGAGRRKNKHIASQYRHMIVVSDGIPTASLETNDSSRYQHGSNVESAAVFRCSNDNGIVLKFGRENASLDESKGSMLNLMNHKRNVDASGNNCRENGEEESSLCVSSVTNGHTRGNELFESEQNRSKPMQSYPAPSWIIPMNQSWNNVTSMVQSSMQMCNPYGIDPTSMQWCHAPRVAVTNIFTPNIGLQFVPASNRNGTPLYGSGNGNVSIGSNGCISPSSSTTSNSCISGNGTPILGKHT