AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47467

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47467.t1 MTR_8g067635 87.245 196 24 1 1 195 253 448 4.22e-121 354
MS.gene47467.t1 MTR_8g067650 87.565 193 23 1 1 192 243 435 1.04e-119 350
MS.gene47467.t1 MTR_2g024380 48.357 213 79 3 1 212 184 366 1.18e-56 186
MS.gene47467.t1 MTR_8g069935 58.182 165 68 1 1 164 234 398 8.54e-56 184
MS.gene47467.t1 MTR_2g024350 60.976 123 48 0 90 212 1 123 5.66e-49 160
MS.gene47467.t1 MTR_6g053200 44.444 216 98 3 1 216 248 441 1.07e-48 167
MS.gene47467.t1 MTR_7g104980 56.566 99 43 0 118 216 18 116 8.18e-29 107
MS.gene47467.t1 MTR_1g099600 48.958 96 48 1 77 172 165 259 4.46e-27 105
MS.gene47467.t1 MTR_7g079040 48.421 95 48 1 75 169 132 225 4.04e-24 97.8
MS.gene47467.t1 MTR_8g028565 49.474 95 47 1 75 169 133 226 4.22e-24 97.8
MS.gene47467.t1 MTR_8g098945 59.722 72 28 1 98 169 130 200 2.57e-22 92.0
MS.gene47467.t1 MTR_7g105000 41.176 119 64 3 1 117 226 340 1.06e-20 89.0
MS.gene47467.t1 MTR_7g109600 43.519 108 56 1 56 158 7 114 2.98e-20 86.3
MS.gene47467.t1 MTR_4g094908 49.398 83 41 1 83 165 253 334 4.18e-19 84.3
MS.gene47467.t1 MTR_2g032030 48.101 79 40 1 95 173 265 342 8.24e-19 84.3
MS.gene47467.t1 MTR_1g070455 52.941 85 35 2 79 159 276 359 1.41e-18 83.2
MS.gene47467.t1 MTR_5g016610 45.361 97 47 2 58 154 166 256 1.43e-18 82.0
MS.gene47467.t1 MTR_2g032030 48.101 79 40 1 95 173 265 342 1.58e-18 83.6
MS.gene47467.t1 MTR_4g127040 52.632 76 35 1 79 154 228 302 2.07e-18 82.0
MS.gene47467.t1 MTR_2g016770 56.667 60 26 0 98 157 36 95 1.11e-17 78.6
MS.gene47467.t1 MTR_2g016770 56.667 60 26 0 98 157 28 87 1.21e-17 78.2
MS.gene47467.t1 MTR_8g087000 53.226 62 29 0 98 159 78 139 1.40e-17 79.0
MS.gene47467.t1 MTR_2g083870 58.333 60 25 0 98 157 81 140 1.45e-17 79.0
MS.gene47467.t1 MTR_8g087000 53.226 62 29 0 98 159 77 138 1.70e-17 78.6
MS.gene47467.t1 MTR_1g009613 46.154 91 45 2 79 166 245 334 4.26e-17 79.0
MS.gene47467.t1 MTR_7g080020 47.191 89 45 2 87 175 270 356 1.06e-16 78.6
MS.gene47467.t1 MTR_7g080020 44.048 84 45 2 86 168 481 563 3.02e-14 71.2
MS.gene47467.t1 MTR_3g108960 48.780 82 37 2 77 154 291 371 2.28e-16 77.0
MS.gene47467.t1 MTR_7g100510 41.964 112 63 2 82 193 174 283 4.09e-16 76.6
MS.gene47467.t1 MTR_7g100510 42.105 76 43 1 98 173 362 436 2.07e-14 71.6
MS.gene47467.t1 MTR_0117s0040 36.522 115 69 2 83 194 61 174 6.83e-16 74.3
MS.gene47467.t1 MTR_7g011820 40.741 108 51 3 64 160 184 289 7.14e-16 75.9
MS.gene47467.t1 MTR_7g011820 53.226 62 28 1 97 158 410 470 7.27e-15 73.2
MS.gene47467.t1 MTR_4g082580 38.776 98 54 2 95 192 276 367 7.52e-16 75.5
MS.gene47467.t1 MTR_4g082580 39.394 99 55 2 88 182 136 233 3.36e-13 67.8
MS.gene47467.t1 MTR_7g080000 42.105 95 53 2 87 181 224 316 1.33e-15 75.5
MS.gene47467.t1 MTR_7g080000 48.438 64 32 1 95 158 430 492 3.47e-13 68.2
MS.gene47467.t1 MTR_7g071120 52.778 72 32 2 89 160 158 227 2.49e-15 74.3
MS.gene47467.t1 MTR_7g071120 46.875 64 33 1 95 158 322 384 3.49e-11 62.4
MS.gene47467.t1 MTR_3g009470 43.689 103 54 4 66 168 251 349 3.01e-15 74.3
MS.gene47467.t1 MTR_3g009470 48.438 64 32 1 95 158 493 555 2.64e-13 68.6
MS.gene47467.t1 MTR_3g009470 43.689 103 54 4 66 168 251 349 5.65e-15 73.6
MS.gene47467.t1 MTR_3g056100 52.941 68 30 2 90 157 236 301 8.62e-15 72.8
MS.gene47467.t1 MTR_3g056100 44.928 69 37 1 95 163 413 480 1.12e-12 66.6
MS.gene47467.t1 MTR_3g056100 52.941 68 30 2 90 157 92 157 8.70e-15 72.8
MS.gene47467.t1 MTR_3g056100 44.928 69 37 1 95 163 269 336 1.14e-12 66.6
MS.gene47467.t1 MTR_3g056100 52.941 68 30 2 90 157 236 301 9.23e-15 72.8
MS.gene47467.t1 MTR_3g056100 44.928 69 37 1 95 163 400 467 1.12e-12 66.6
MS.gene47467.t1 MTR_3g056100 52.941 68 30 2 90 157 92 157 9.46e-15 72.8
MS.gene47467.t1 MTR_3g056100 44.928 69 37 1 95 163 256 323 1.19e-12 66.6
MS.gene47467.t1 MTR_5g042920 33.065 124 65 2 66 172 272 394 1.62e-14 72.0
MS.gene47467.t1 MTR_5g042920 38.400 125 66 4 88 212 142 255 1.27e-13 69.3
MS.gene47467.t1 MTR_5g091390 51.613 62 29 1 97 158 157 217 1.83e-14 71.2
MS.gene47467.t1 MTR_3g031220 39.167 120 55 5 47 160 152 259 2.23e-14 71.2
MS.gene47467.t1 MTR_3g031220 39.167 120 55 5 47 160 152 259 2.36e-14 71.6
MS.gene47467.t1 MTR_3g031220 46.875 64 33 1 95 158 363 425 1.01e-11 63.9
MS.gene47467.t1 MTR_5g097490 49.275 69 31 3 93 160 88 153 5.39e-14 70.1
MS.gene47467.t1 MTR_5g097490 43.836 73 40 1 86 158 241 312 1.02e-11 63.5
MS.gene47467.t1 MTR_4g130900 49.206 63 31 1 95 157 357 418 6.95e-14 70.1
MS.gene47467.t1 MTR_4g094588 38.947 95 52 3 83 177 165 253 7.81e-14 69.7
MS.gene47467.t1 MTR_1g086790 37.778 90 54 2 77 166 139 226 8.40e-14 69.3
MS.gene47467.t1 MTR_5g074400 53.226 62 27 2 96 157 242 301 2.59e-13 68.6
MS.gene47467.t1 MTR_5g074400 41.176 85 49 1 74 158 397 480 7.77e-13 67.0
MS.gene47467.t1 MTR_4g007060 34.783 115 67 2 95 209 126 232 3.32e-13 67.4
MS.gene47467.t1 MTR_7g062220 46.053 76 40 1 79 154 106 180 3.81e-13 67.4
MS.gene47467.t1 MTR_7g109800 38.202 89 53 2 77 165 126 212 5.01e-13 67.0
MS.gene47467.t1 MTR_4g007060 34.783 115 67 2 95 209 160 266 5.05e-13 67.0
MS.gene47467.t1 MTR_5g094430 46.875 64 33 1 95 158 362 424 7.40e-13 67.4
MS.gene47467.t1 MTR_5g094430 53.226 62 27 2 96 157 199 258 1.23e-12 66.6
MS.gene47467.t1 MTR_5g094430 46.875 64 33 1 95 158 228 290 8.48e-13 67.0
MS.gene47467.t1 MTR_5g094430 53.226 62 27 2 96 157 65 124 1.26e-12 66.2
MS.gene47467.t1 MTR_5g094430 46.875 64 33 1 95 158 250 312 8.54e-13 67.0
MS.gene47467.t1 MTR_5g094430 53.226 62 27 2 96 157 87 146 1.25e-12 66.2
MS.gene47467.t1 MTR_1g077780 32.353 102 62 3 83 184 49 143 8.79e-13 63.9
MS.gene47467.t1 MTR_4g122530 40.789 76 43 2 83 158 195 268 9.03e-13 66.6
MS.gene47467.t1 MTR_2g105060 35.514 107 60 1 95 192 92 198 1.35e-12 65.1
MS.gene47467.t1 MTR_7g079010 36.667 90 54 2 67 154 109 197 1.38e-12 64.7
MS.gene47467.t1 MTR_3g095040 42.647 68 38 1 94 161 115 181 1.52e-12 64.3
MS.gene47467.t1 MTR_2g105060 35.514 107 60 1 95 192 167 273 3.02e-12 64.7
MS.gene47467.t1 MTR_2g033820 46.774 62 32 1 97 158 203 263 3.35e-12 65.1
MS.gene47467.t1 MTR_2g045360 45.000 60 33 0 98 157 212 271 3.79e-12 65.1
MS.gene47467.t1 MTR_4g013260 47.619 63 32 1 95 157 334 395 5.18e-12 64.7
MS.gene47467.t1 MTR_4g013260 34.677 124 74 4 39 157 122 243 5.23e-12 64.7
MS.gene47467.t1 MTR_8g007270 44.928 69 36 2 95 163 152 218 6.88e-12 62.8
MS.gene47467.t1 MTR_5g043880 36.275 102 61 3 55 154 81 180 7.55e-12 63.5
MS.gene47467.t1 MTR_3g106060 31.884 138 72 4 29 154 68 195 1.06e-11 63.2
MS.gene47467.t1 MTR_3g053330 36.264 91 55 2 83 171 238 327 1.76e-11 63.2
MS.gene47467.t1 MTR_3g093830 35.345 116 55 4 66 168 103 211 1.84e-11 62.8
MS.gene47467.t1 MTR_1g013790 43.284 67 38 0 95 161 99 165 2.85e-11 61.6
MS.gene47467.t1 MTR_5g067680 50.000 58 27 1 97 152 202 259 3.19e-11 62.0
MS.gene47467.t1 MTR_8g099350 46.552 58 29 1 97 152 132 189 5.59e-11 61.2
MS.gene47467.t1 MTR_1g013760 47.368 57 30 0 98 154 141 197 6.74e-11 60.8
MS.gene47467.t1 MTR_7g028415 37.662 77 45 2 95 169 91 166 7.29e-11 59.3
MS.gene47467.t1 MTR_7g028710 37.662 77 45 2 95 169 91 166 7.29e-11 59.3
MS.gene47467.t1 MTR_3g090860 40.789 76 39 2 97 170 133 204 7.71e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47467.t1 AT4G01250 40.252 159 78 6 16 174 63 204 1.20e-26 103
MS.gene47467.t1 AT5G52830 46.875 96 50 1 77 172 145 239 2.20e-24 99.0
MS.gene47467.t1 AT4G23550 48.387 93 47 1 81 173 124 215 2.36e-23 95.5
MS.gene47467.t1 AT5G45050 50.000 82 41 0 77 158 1021 1102 1.14e-20 90.1
MS.gene47467.t1 AT5G45050 50.000 82 41 0 77 158 1160 1241 1.15e-20 90.1
MS.gene47467.t1 AT5G45050 50.000 82 41 0 77 158 1132 1213 1.22e-20 90.1
MS.gene47467.t1 AT4G31550 48.315 89 43 1 77 162 222 310 4.34e-19 84.3
MS.gene47467.t1 AT4G31550 46.739 92 48 1 71 162 221 311 9.32e-19 83.2
MS.gene47467.t1 AT2G24570 50.000 84 41 1 71 154 218 300 1.42e-18 82.8
MS.gene47467.t1 AT2G34830 53.968 63 29 0 95 157 213 275 5.72e-18 82.0
MS.gene47467.t1 AT4G24240 46.226 106 49 3 56 154 234 338 6.20e-18 81.3
MS.gene47467.t1 AT2G34830 50.000 72 35 1 95 166 222 292 6.31e-18 81.6
MS.gene47467.t1 AT5G28650 52.564 78 36 1 79 156 245 321 7.33e-18 80.9
MS.gene47467.t1 AT2G23320 53.846 65 30 0 90 154 233 297 8.07e-18 80.5
MS.gene47467.t1 AT3G04670 52.564 78 36 1 79 156 245 321 9.79e-18 80.5
MS.gene47467.t1 AT3G58710 51.515 66 32 0 98 163 71 136 1.01e-17 79.7
MS.gene47467.t1 AT3G58710 51.515 66 32 0 98 163 72 137 1.01e-17 79.7
MS.gene47467.t1 AT1G29280 56.667 60 26 0 98 157 75 134 1.32e-17 79.3
MS.gene47467.t1 AT1G30650 39.623 106 58 1 58 157 172 277 1.80e-17 80.5
MS.gene47467.t1 AT5G07100 39.655 116 63 3 90 199 17 131 1.32e-16 75.9
MS.gene47467.t1 AT5G07100 46.875 64 33 1 95 158 139 201 2.10e-13 67.0
MS.gene47467.t1 AT5G07100 39.655 116 63 3 90 199 17 131 1.32e-16 75.9
MS.gene47467.t1 AT5G07100 46.875 64 33 1 95 158 139 201 2.10e-13 67.0
MS.gene47467.t1 AT5G07100 39.655 116 63 3 90 199 17 131 1.32e-16 75.9
MS.gene47467.t1 AT5G07100 46.875 64 33 1 95 158 139 201 2.10e-13 67.0
MS.gene47467.t1 AT5G07100 39.655 116 63 3 90 199 17 131 1.32e-16 75.9
MS.gene47467.t1 AT5G07100 46.875 64 33 1 95 158 139 201 2.10e-13 67.0
MS.gene47467.t1 AT2G30590 54.545 66 30 0 91 156 307 372 1.48e-16 77.8
MS.gene47467.t1 AT5G07100 39.655 116 63 3 90 199 110 224 3.08e-16 76.3
MS.gene47467.t1 AT5G07100 46.875 64 33 1 95 158 232 294 3.12e-13 67.8
MS.gene47467.t1 AT3G01080 43.820 89 41 3 93 174 201 287 9.24e-16 75.1
MS.gene47467.t1 AT2G30250 39.844 128 57 7 47 157 100 224 9.94e-16 75.5
MS.gene47467.t1 AT2G30250 50.820 61 29 1 98 158 329 388 1.77e-12 65.9
MS.gene47467.t1 AT4G26440 32.143 140 79 2 28 167 318 441 1.34e-15 75.5
MS.gene47467.t1 AT4G26440 50.685 73 34 2 96 168 177 247 4.16e-15 73.9
MS.gene47467.t1 AT3G01080 41.667 96 48 3 93 182 163 256 1.37e-15 75.1
MS.gene47467.t1 AT3G01080 50.794 63 30 1 95 157 304 365 9.81e-15 72.4
MS.gene47467.t1 AT3G01080 43.820 89 41 3 93 174 201 287 1.78e-15 74.7
MS.gene47467.t1 AT3G01080 50.794 63 30 1 95 157 342 403 9.34e-15 72.8
MS.gene47467.t1 AT2G03340 49.254 67 33 1 97 163 415 480 5.27e-15 73.6
MS.gene47467.t1 AT2G03340 46.237 93 42 4 92 178 245 335 1.01e-13 69.7
MS.gene47467.t1 AT5G56270 42.308 104 55 3 96 196 272 373 2.15e-14 72.0
MS.gene47467.t1 AT5G56270 48.438 64 32 1 95 158 485 547 4.01e-13 68.2
MS.gene47467.t1 AT2G38470 44.211 95 48 4 64 157 152 242 2.64e-14 71.6
MS.gene47467.t1 AT2G38470 48.438 64 32 1 95 158 360 422 4.23e-13 67.8
MS.gene47467.t1 AT1G13960 42.222 90 44 4 75 159 203 289 3.11e-14 71.2
MS.gene47467.t1 AT1G13960 47.619 63 32 1 95 157 407 468 2.30e-13 68.6
MS.gene47467.t1 AT1G13960 42.222 90 44 4 75 159 50 136 3.56e-14 70.9
MS.gene47467.t1 AT1G13960 47.619 63 32 1 95 157 254 315 2.40e-13 68.2
MS.gene47467.t1 AT2G04880 41.860 86 49 1 72 157 258 342 1.03e-13 69.7
MS.gene47467.t1 AT2G04880 46.154 65 33 2 93 157 107 169 8.80e-12 63.9
MS.gene47467.t1 AT2G04880 41.860 86 49 1 72 157 282 366 1.05e-13 69.7
MS.gene47467.t1 AT2G04880 46.154 65 33 2 93 157 107 169 8.91e-12 63.9
MS.gene47467.t1 AT5G41570 35.135 111 67 3 71 177 70 179 1.50e-13 66.6
MS.gene47467.t1 AT4G18170 40.964 83 47 2 77 159 153 233 1.79e-13 68.6
MS.gene47467.t1 AT2G47260 41.975 81 45 2 77 157 155 233 1.86e-13 68.6
MS.gene47467.t1 AT2G21900 51.613 62 29 1 97 158 109 169 1.95e-13 67.0
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 209 309 2.48e-13 68.6
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 379 446 8.35e-12 64.3
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 137 237 2.72e-13 68.6
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 307 374 9.41e-12 63.9
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 33 133 2.80e-13 68.2
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 203 270 9.09e-12 63.9
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 33 133 2.80e-13 68.2
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 203 270 9.09e-12 63.9
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 33 133 2.80e-13 68.2
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 203 270 9.09e-12 63.9
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 33 133 2.80e-13 68.2
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 203 270 9.09e-12 63.9
MS.gene47467.t1 AT4G26640 39.048 105 57 4 95 196 169 269 3.01e-13 68.6
MS.gene47467.t1 AT4G26640 42.029 69 39 1 95 163 339 406 9.20e-12 63.9
MS.gene47467.t1 AT2G37260 42.683 82 41 3 77 154 326 405 3.16e-13 68.2
MS.gene47467.t1 AT2G37260 50.746 67 31 2 92 158 160 224 7.22e-13 67.0
MS.gene47467.t1 AT2G37260 42.683 82 41 3 77 154 244 323 3.38e-13 67.8
MS.gene47467.t1 AT2G37260 50.746 67 31 2 92 158 78 142 8.35e-13 66.6
MS.gene47467.t1 AT2G37260 42.683 82 41 3 77 154 246 325 3.47e-13 67.8
MS.gene47467.t1 AT2G37260 50.746 67 31 2 92 158 80 144 8.10e-13 66.6
MS.gene47467.t1 AT1G64000 39.241 79 47 1 76 154 93 170 5.18e-13 65.5
MS.gene47467.t1 AT4G30935 40.698 86 44 3 74 158 312 391 6.74e-13 67.4
MS.gene47467.t1 AT5G46350 41.558 77 43 2 83 159 170 244 1.02e-12 66.2
MS.gene47467.t1 AT1G55600 51.667 60 28 1 98 157 308 366 1.13e-12 66.6
MS.gene47467.t1 AT5G45260 35.922 103 62 3 75 174 1185 1286 1.47e-12 66.6
MS.gene47467.t1 AT1G29860 39.286 84 49 2 83 166 123 204 2.05e-12 65.1
MS.gene47467.t1 AT2G46130 41.026 78 44 2 77 154 11 86 2.47e-12 61.6
MS.gene47467.t1 AT3G01970 42.466 73 39 2 95 165 63 134 2.63e-12 62.8
MS.gene47467.t1 AT1G69310 38.636 88 52 2 68 155 119 204 2.67e-12 64.7
MS.gene47467.t1 AT1G69310 38.636 88 52 2 68 155 119 204 2.67e-12 64.7
MS.gene47467.t1 AT1G69310 38.636 88 52 2 68 155 119 204 2.67e-12 64.7
MS.gene47467.t1 AT3G62340 36.145 83 50 2 77 157 96 177 3.22e-12 64.7
MS.gene47467.t1 AT5G43290 49.206 63 31 1 92 154 109 170 3.93e-12 64.3
MS.gene47467.t1 AT1G80840 42.188 64 37 0 95 158 144 207 4.03e-12 64.3
MS.gene47467.t1 AT5G26170 45.902 61 32 1 94 154 110 169 5.30e-12 62.4
MS.gene47467.t1 AT5G49520 45.902 61 32 1 97 157 221 280 5.75e-12 64.3
MS.gene47467.t1 AT5G13080 37.037 81 48 2 95 173 65 144 6.65e-12 61.6
MS.gene47467.t1 AT4G31800 40.449 89 49 2 97 185 111 195 6.81e-12 63.2
MS.gene47467.t1 AT2G44745 46.667 60 31 1 95 154 143 201 7.48e-12 62.8
MS.gene47467.t1 AT2G25000 37.349 83 47 1 80 157 81 163 8.05e-12 62.8
MS.gene47467.t1 AT2G25000 37.349 83 47 1 80 157 81 163 8.05e-12 62.8
MS.gene47467.t1 AT2G25000 37.349 83 47 1 80 157 103 185 1.03e-11 62.8
MS.gene47467.t1 AT4G31800 40.909 88 48 2 98 185 177 260 1.27e-11 63.2
MS.gene47467.t1 AT2G25000 37.349 83 47 1 80 157 124 206 1.70e-11 62.4
MS.gene47467.t1 AT1G80590 47.619 63 31 1 98 158 86 148 2.50e-11 61.6
MS.gene47467.t1 AT1G66550 49.123 57 27 1 98 152 109 165 4.84e-11 60.8
MS.gene47467.t1 AT1G66550 49.123 57 27 1 98 152 104 160 5.36e-11 60.8
MS.gene47467.t1 AT4G04450 37.079 89 50 2 98 180 199 287 7.62e-11 61.2
MS.gene47467.t1 AT5G01900 50.000 60 28 1 95 152 108 167 9.37e-11 60.1

Find 45 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAATTTGATGACATATTC+TGG 0.180052 8.4:+35198058 None:intergenic
CGAAAACAGACACTCGATTT+TGG 0.221712 8.4:+35199603 None:intergenic
ACAGAAAACCAGCAAATAAT+TGG 0.261265 8.4:-35198101 MS.gene47467:CDS
TGGGGTTCTTGTGGTTCTAA+TGG 0.273735 8.4:+35199524 None:intergenic
TGGATGTGGAGACCCTTTAA+TGG 0.287579 8.4:+35199183 None:intergenic
TAAAGTTGTAGAACTGGTTT+GGG 0.305703 8.4:+35199469 None:intergenic
CAGAATATGTCATCAAATTC+AGG 0.324509 8.4:-35198057 MS.gene47467:CDS
GTTGAGTTCAGGATGAAAAT+TGG 0.333089 8.4:+35199550 None:intergenic
TCATGGAGATGGAGAAAATA+TGG 0.338703 8.4:-35199214 MS.gene47467:CDS
ATAAGATTTCAGAAGATTCA+TGG 0.343013 8.4:-35199231 MS.gene47467:CDS
GTAAAGTTGTAGAACTGGTT+TGG 0.359458 8.4:+35199468 None:intergenic
ACGGAATTCCAATTATTTGC+TGG 0.359995 8.4:+35198093 None:intergenic
GTAGTCGATTATGATTTCTC+TGG 0.378001 8.4:+35199504 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.385486 8.4:+35199184 None:intergenic
ACTCGAGCCTTGCTGCTCTC+TGG 0.411380 8.4:+35197949 None:intergenic
TTGAGCAACATCCAGCTTCT+CGG 0.416229 8.4:+35198022 None:intergenic
ATTCTGTAAAGTTGTAGAAC+TGG 0.417920 8.4:+35199463 None:intergenic
TCTAATGGATGGTTGAGTTC+AGG 0.423106 8.4:+35199539 None:intergenic
TTGCTCAAACGTTAGAAACT+TGG 0.430903 8.4:-35198007 MS.gene47467:CDS
TAGTCGATTATGATTTCTCT+GGG 0.436337 8.4:+35199505 None:intergenic
TGCTGCAACATTACCACATT+AGG 0.438405 8.4:+35197979 None:intergenic
ACTTATGAAGTGACATATAT+AGG 0.438664 8.4:-35198135 MS.gene47467:CDS
GTTCTTGTGGTTCTAATGGA+TGG 0.440210 8.4:+35199528 None:intergenic
TCATCAAATTCAGGATTAGA+TGG 0.442631 8.4:-35198048 MS.gene47467:CDS
TGAATTTGATGACATATTCT+GGG 0.443651 8.4:+35198059 None:intergenic
TGTTGTTGTTCAACGAGCAT+TGG 0.450106 8.4:+35199638 None:intergenic
TTAGATGGTGTCCGAGAAGC+TGG 0.454965 8.4:-35198033 MS.gene47467:CDS
AATGCTCGTTGAACAACAAC+AGG 0.486815 8.4:-35199636 MS.gene47467:CDS
TGCTCAAACGTTAGAAACTT+GGG 0.493239 8.4:-35198006 MS.gene47467:CDS
CTGGTTTGGGGTAGTAGAAT+CGG 0.494141 8.4:+35199482 None:intergenic
TGATTTCTCTGGGGTTCTTG+TGG 0.495150 8.4:+35199515 None:intergenic
AATAATTGGAATTCCGTTGT+TGG 0.498386 8.4:-35198087 MS.gene47467:CDS
TTTCAGAAGATTCATGGAGA+TGG 0.509856 8.4:-35199225 MS.gene47467:CDS
AAATAATAACCAAGTGACAT+TGG 0.536349 8.4:-35199272 MS.gene47467:CDS
CATAGCTCACCAATGTCACT+TGG 0.536490 8.4:+35199263 None:intergenic
GGTGGTTATAGTCTTAATGC+AGG 0.545859 8.4:+35199571 None:intergenic
AAACTTGGGTTCTCCTAATG+TGG 0.561663 8.4:-35197992 MS.gene47467:CDS
GAGTTCAGGATGAAAATTGG+TGG 0.568251 8.4:+35199553 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.584498 8.4:-35199180 MS.gene47467:intron
AGCAAGGCTCGAGTTCATGA+TGG 0.606224 8.4:-35197940 MS.gene47467:CDS
ATTCTGGGAGCTACCAACAA+CGG 0.652456 8.4:+35198074 None:intergenic
TGATCATCCAGAGAGCAGCA+AGG 0.696293 8.4:-35197956 MS.gene47467:CDS
GACATTGGTGAGCTATGTGA+AGG 0.706817 8.4:-35199257 MS.gene47467:CDS
AGTCGATTATGATTTCTCTG+GGG 0.721816 8.4:+35199506 None:intergenic
AAAGTTGTAGAACTGGTTTG+GGG 0.728163 8.4:+35199470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAATTATATATTTTTA+GGG - chr8.4:35199069-35199088 MS.gene47467:intron 0.0%
!!! TTAAAAAATTATATATTTTT+AGG - chr8.4:35199068-35199087 MS.gene47467:intron 0.0%
!! AAAAAATGATGTAAATGTAA+GGG + chr8.4:35199183-35199202 None:intergenic 15.0%
!! AGGGTTAAAATTAATATTAA+AGG + chr8.4:35198920-35198939 None:intergenic 15.0%
!! TAAAAAATGATGTAAATGTA+AGG + chr8.4:35199184-35199203 None:intergenic 15.0%
!!! CAATATGAATAAATTTTGTA+GGG - chr8.4:35198968-35198987 MS.gene47467:intron 15.0%
!!! TATATGTATCATTTTTTGTT+TGG - chr8.4:35198758-35198777 MS.gene47467:intron 15.0%
!! AAAAATGATGTAAATGTAAG+GGG + chr8.4:35199182-35199201 None:intergenic 20.0%
!! AAAAATTTAAACCAGGAAAA+AGG - chr8.4:35198144-35198163 MS.gene47467:CDS 20.0%
!! AAACAACAAAAATTTAAACC+AGG - chr8.4:35198137-35198156 MS.gene47467:CDS 20.0%
!! CAAAAAATGATACATATACA+AGG + chr8.4:35198756-35198775 None:intergenic 20.0%
!! CCTACAAAATTTATTCATAT+TGG + chr8.4:35198970-35198989 None:intergenic 20.0%
!! TCATATAGACACATATAAAT+AGG + chr8.4:35199269-35199288 None:intergenic 20.0%
!! TTTGAATGAATAAAAAACCT+TGG + chr8.4:35198408-35198427 None:intergenic 20.0%
!!! AATTTTAACCCTTATATTGA+TGG - chr8.4:35198928-35198947 MS.gene47467:intron 20.0%
!!! ATTTTAACCCATCAATATAA+GGG + chr8.4:35198939-35198958 None:intergenic 20.0%
!!! ATTTTAACCCTTATATTGAT+GGG - chr8.4:35198929-35198948 MS.gene47467:intron 20.0%
!!! ATTTTTTCTACAAGAAAAGA+AGG + chr8.4:35198280-35198299 None:intergenic 20.0%
!!! CCAATATGAATAAATTTTGT+AGG - chr8.4:35198967-35198986 MS.gene47467:intron 20.0%
!!! GGTTTAAATTTTTGTTGTTT+CGG + chr8.4:35198137-35198156 None:intergenic 20.0%
!!! TTTTTTCTACAAGAAAAGAA+GGG + chr8.4:35198279-35198298 None:intergenic 20.0%
! AAATAATAACCAAGTGACAT+TGG - chr8.4:35198296-35198315 MS.gene47467:intron 25.0%
! ACTTATGAAGTGACATATAT+AGG - chr8.4:35199433-35199452 MS.gene47467:CDS 25.0%
! ATAAGATTTCAGAAGATTCA+TGG - chr8.4:35198337-35198356 MS.gene47467:intron 25.0%
! CAAACACTGAAATAAAAATG+TGG - chr8.4:35199005-35199024 MS.gene47467:intron 25.0%
! TCAAAAACAAAAGATCAAAG+AGG + chr8.4:35198814-35198833 None:intergenic 25.0%
! TTGTTTAGCAAAAAAACTAG+TGG - chr8.4:35199387-35199406 MS.gene47467:intron 25.0%
!! AAGAAGGGTAAGATAATTTT+CGG + chr8.4:35198264-35198283 None:intergenic 25.0%
!! ACTAATTAAAACCATTGGTT+TGG + chr8.4:35198507-35198526 None:intergenic 25.0%
!! TGAATTTGATGACATATTCT+GGG + chr8.4:35199512-35199531 None:intergenic 25.0%
!!! CTAATTAAAACCATTGGTTT+GGG + chr8.4:35198506-35198525 None:intergenic 25.0%
!!! GATTTTAACCCATCAATATA+AGG + chr8.4:35198940-35198959 None:intergenic 25.0%
AAGAATGATCAAAATGCATG+TGG + chr8.4:35198532-35198551 None:intergenic 30.0%
AATAATTGGAATTCCGTTGT+TGG - chr8.4:35199481-35199500 MS.gene47467:CDS 30.0%
ACAGAAAACCAGCAAATAAT+TGG - chr8.4:35199467-35199486 MS.gene47467:CDS 30.0%
ACCAAGCATTAAAGAACAAA+GGG + chr8.4:35198853-35198872 None:intergenic 30.0%
ATGGACAAAAACCCATTAAA+GGG - chr8.4:35198373-35198392 MS.gene47467:intron 30.0%
ATTCTGTAAAGTTGTAGAAC+TGG + chr8.4:35198108-35198127 None:intergenic 30.0%
CAGAATATGTCATCAAATTC+AGG - chr8.4:35199511-35199530 MS.gene47467:CDS 30.0%
CTAGTGGAGAAAATAAAAAC+TGG - chr8.4:35199403-35199422 MS.gene47467:intron 30.0%
GACAAATATGATCTTGATAG+TGG - chr8.4:35198577-35198596 MS.gene47467:intron 30.0%
TACCAAGCATTAAAGAACAA+AGG + chr8.4:35198854-35198873 None:intergenic 30.0%
TAGTCGATTATGATTTCTCT+GGG + chr8.4:35198066-35198085 None:intergenic 30.0%
TATGGACAAAAACCCATTAA+AGG - chr8.4:35198372-35198391 MS.gene47467:intron 30.0%
TCATCAAATTCAGGATTAGA+TGG - chr8.4:35199520-35199539 MS.gene47467:CDS 30.0%
TGAATAAAAAACCTTGGATG+TGG + chr8.4:35198402-35198421 None:intergenic 30.0%
TGGTCACTAATTAAAACCAT+TGG + chr8.4:35198512-35198531 None:intergenic 30.0%
TGTAAATGTAAGGGGAAAAT+AGG + chr8.4:35199174-35199193 None:intergenic 30.0%
! CTGAATTTGATGACATATTC+TGG + chr8.4:35199513-35199532 None:intergenic 30.0%
! TAAAGTTGTAGAACTGGTTT+GGG + chr8.4:35198102-35198121 None:intergenic 30.0%
! TCAATTCTTTACCTTTTTCC+TGG + chr8.4:35198158-35198177 None:intergenic 30.0%
ACGGAATTCCAATTATTTGC+TGG + chr8.4:35199478-35199497 None:intergenic 35.0%
AGTCGATTATGATTTCTCTG+GGG + chr8.4:35198065-35198084 None:intergenic 35.0%
ATGTATTATTTCACGTGCGT+GGG - chr8.4:35199206-35199225 MS.gene47467:CDS 35.0%
CAATCAATGAATGACTTTGC+AGG - chr8.4:35199335-35199354 MS.gene47467:intron 35.0%
CCAAGCATTAAAGAACAAAG+GGG + chr8.4:35198852-35198871 None:intergenic 35.0%
GGAGAAAATAAAAACTGGAG+AGG - chr8.4:35199408-35199427 MS.gene47467:intron 35.0%
GTAAAGTTGTAGAACTGGTT+TGG + chr8.4:35198103-35198122 None:intergenic 35.0%
GTAGTCGATTATGATTTCTC+TGG + chr8.4:35198067-35198086 None:intergenic 35.0%
GTTGAGTTCAGGATGAAAAT+TGG + chr8.4:35198021-35198040 None:intergenic 35.0%
TATGTATTATTTCACGTGCG+TGG - chr8.4:35199205-35199224 MS.gene47467:CDS 35.0%
TCATGGAGATGGAGAAAATA+TGG - chr8.4:35198354-35198373 MS.gene47467:intron 35.0%
TGCTCAAACGTTAGAAACTT+GGG - chr8.4:35199562-35199581 MS.gene47467:CDS 35.0%
TTGCTCAAACGTTAGAAACT+TGG - chr8.4:35199561-35199580 MS.gene47467:CDS 35.0%
TTTCAGAAGATTCATGGAGA+TGG - chr8.4:35198343-35198362 MS.gene47467:intron 35.0%
!! AAAGTTGTAGAACTGGTTTG+GGG + chr8.4:35198101-35198120 None:intergenic 35.0%
AAACTTGGGTTCTCCTAATG+TGG - chr8.4:35199576-35199595 MS.gene47467:CDS 40.0%
CCCCTTTGTTCTTTAATGCT+TGG - chr8.4:35198849-35198868 MS.gene47467:intron 40.0%
GAGTTCAGGATGAAAATTGG+TGG + chr8.4:35198018-35198037 None:intergenic 40.0%
TGCTGCAACATTACCACATT+AGG + chr8.4:35199592-35199611 None:intergenic 40.0%
! CGAAAACAGACACTCGATTT+TGG + chr8.4:35197968-35197987 None:intergenic 40.0%
! GGTGGTTATAGTCTTAATGC+AGG + chr8.4:35198000-35198019 None:intergenic 40.0%
! TCTAATGGATGGTTGAGTTC+AGG + chr8.4:35198032-35198051 None:intergenic 40.0%
!! AAAACCATTGGTTTGGGTGT+GGG + chr8.4:35198500-35198519 None:intergenic 40.0%
!! GTTCTTGTGGTTCTAATGGA+TGG + chr8.4:35198043-35198062 None:intergenic 40.0%
!! TAAAACCATTGGTTTGGGTG+TGG + chr8.4:35198501-35198520 None:intergenic 40.0%
ATTATTTCACGTGCGTGGGA+CGG - chr8.4:35199210-35199229 MS.gene47467:CDS 45.0%
CATAGCTCACCAATGTCACT+TGG + chr8.4:35198308-35198327 None:intergenic 45.0%
GACATTGGTGAGCTATGTGA+AGG - chr8.4:35198311-35198330 MS.gene47467:intron 45.0%
GGATGTGGAGACCCTTTAAT+GGG + chr8.4:35198387-35198406 None:intergenic 45.0%
TGGATGTGGAGACCCTTTAA+TGG + chr8.4:35198388-35198407 None:intergenic 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr8.4:35198388-35198407 MS.gene47467:intron 45.0%
TTGAGCAACATCCAGCTTCT+CGG + chr8.4:35199549-35199568 None:intergenic 45.0%
! ATTCTGGGAGCTACCAACAA+CGG + chr8.4:35199497-35199516 None:intergenic 45.0%
!! CTGGTTTGGGGTAGTAGAAT+CGG + chr8.4:35198089-35198108 None:intergenic 45.0%
!! TGATTTCTCTGGGGTTCTTG+TGG + chr8.4:35198056-35198075 None:intergenic 45.0%
!! TGGGGTTCTTGTGGTTCTAA+TGG + chr8.4:35198047-35198066 None:intergenic 45.0%
!! TATAAAATATATTAAATAGT+AGG + chr8.4:35198183-35198202 None:intergenic 5.0%
!!! ATTTTGTTTTATATTAAAAT+TGG - chr8.4:35198892-35198911 MS.gene47467:intron 5.0%
AGCAAGGCTCGAGTTCATGA+TGG - chr8.4:35199628-35199647 MS.gene47467:CDS 50.0%
TGATCATCCAGAGAGCAGCA+AGG - chr8.4:35199612-35199631 MS.gene47467:CDS 50.0%
TGGGTGTGGGTGTGTGTATT+AGG + chr8.4:35198487-35198506 None:intergenic 50.0%
TTAGATGGTGTCCGAGAAGC+TGG - chr8.4:35199535-35199554 MS.gene47467:CDS 50.0%
CACACCCACACCCAAACCAA+TGG - chr8.4:35198493-35198512 MS.gene47467:intron 55.0%
ACTCGAGCCTTGCTGCTCTC+TGG + chr8.4:35199622-35199641 None:intergenic 60.0%
TCACGTGCGTGGGACGGAGT+TGG - chr8.4:35199216-35199235 MS.gene47467:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 35197933 35199657 35197933 ID=MS.gene47467
chr8.4 mRNA 35197933 35199657 35197933 ID=MS.gene47467.t1;Parent=MS.gene47467
chr8.4 exon 35199425 35199657 35199425 ID=MS.gene47467.t1.exon1;Parent=MS.gene47467.t1
chr8.4 CDS 35199425 35199657 35199425 ID=cds.MS.gene47467.t1;Parent=MS.gene47467.t1
chr8.4 exon 35199181 35199297 35199181 ID=MS.gene47467.t1.exon2;Parent=MS.gene47467.t1
chr8.4 CDS 35199181 35199297 35199181 ID=cds.MS.gene47467.t1;Parent=MS.gene47467.t1
chr8.4 exon 35197933 35198233 35197933 ID=MS.gene47467.t1.exon3;Parent=MS.gene47467.t1
chr8.4 CDS 35197933 35198233 35197933 ID=cds.MS.gene47467.t1;Parent=MS.gene47467.t1
Gene Sequence

>MS.gene47467

ATGCTCGTTGAACAACAACAGGTTCAAGCAAACCAAAATCGAGTGTCTGTTTTCGACTCTATTTCCTGCATTAAGACTATAACCACCAATTTTCATCCTGAACTCAACCATCCATTAGAACCACAAGAACCCCAGAGAAATCATAATCGACTACCGATTCTACTACCCCAAACCAGTTCTACAACTTTACAGAATACAAAACCGAAACAACAAAAATTTAAACCAGGAAAAAGGTAAAGAATTGATTCCTACTATTTAATATATTTTATATGTAAATATATTTCATTTTATTGAAATTATTAAAATACTTTTACATAACTCATTTTTACCGAAAATTATCTTACCCTTCTTTTCTTGTAGAAAAAATAATAACCAAGTGACATTGGTGAGCTATGTGAAGGCAGATAAGATTTCAGAAGATTCATGGAGATGGAGAAAATATGGACAAAAACCCATTAAAGGGTCTCCACATCCAAGGTTTTTTATTCATTCAAAACTCTAACATTATCATAATTTTCTTTTTTTCTAATATTAACACAATATTTTTGTTACCTAATACACACACCCACACCCAAACCAATGGTTTTAATTAGTGACCACATGCATTTTGATCATTCTTATTGAAAAGAGTTTTAGTTAAATGTGACAAATATGATCTTGATAGTGGTTGCGTTTTCTTTTCAATTTCGATAAAAAATTGTTACTCTCATCTTTTCAATGAGAGTCATGTTTGCAAAATATTTTTTTTTTTACAAAGTAAATGTCACTTTTAATTTTCTATGTAATGAGATTAGTTTTTACTTTAATATATGTTTTAAGACCTTGTATATGTATCATTTTTTGTTTGGTAAAAAAAATTATCATTTTTTTGCTTTTAACCTCTTTGATCTTTTGTTTTTGACTATCATAAATATTACCCCTTTGTTCTTTAATGCTTGGTATTTGTTTTAGTTCATGTAATTTTGTTTTATATTAAAATTGGTTCCTTTAATATTAATTTTAACCCTTATATTGATGGGTTAAAATCAAATATTCCAATATGAATAAATTTTGTAGGGACTAAACAAAACAACAAACACTGAAATAAAAATGTGGTATATTTGAAAAAGCTAAACAAATTAGAAAGACTAAATTTTTAAAAAATTATATATTTTTAGGGAACAAAAACATATTTAAATCTCACTTATTTTTCACAACTGTAGAATTGTCTATATCTTTCACTTTAGAATTGTAAAACTCCTATTTTCCCCTTACATTTACATCATTTTTTAATATGTATTATTTCACGTGCGTGGGACGGAGTTGGATTTTAAATATTTTTTTGAAAGCATCGCCTATTTATATGTGTCTATATGATTTTAGATATTAAAATGTTACGATTTAGTGATGTTTGATATAAAAACAATCAATGAATGACTTTGCAGGAGCTACTTCAAATGTAGCAGTTTCAACGATTGTTTAGCAAAAAAACTAGTGGAGAAAATAAAAACTGGAGAGGACACTTATGAAGTGACATATATAGGAGAGCACAATCACAGAAAACCAGCAAATAATTGGAATTCCGTTGTTGGTAGCTCCCAGAATATGTCATCAAATTCAGGATTAGATGGTGTCCGAGAAGCTGGATGTTGCTCAAACGTTAGAAACTTGGGTTCTCCTAATGTGGTAATGTTGCAGCATGATCATCCAGAGAGCAGCAAGGCTCGAGTTCATGATGGTGAATAA
Protein sequence

>MS.gene47467.t1

MLVEQQQVQANQNRVSVFDSISCIKTITTNFHPELNHPLEPQEPQRNHNRLPILLPQTSSTTLQNTKPKQQKFKPGKRKNNNQVTLVSYVKADKISEDSWRWRKYGQKPIKGSPHPRSYFKCSSFNDCLAKKLVEKIKTGEDTYEVTYIGEHNHRKPANNWNSVVGSSQNMSSNSGLDGVREAGCCSNVRNLGSPNVVMLQHDHPESSKARVHDGE