Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47797.t1 | RHN45637.1 | 86.5 | 96 | 13 | 0 | 1 | 96 | 181 | 276 | 1.70E-41 | 178.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47797.t1 | Q6AV21 | 42.0 | 69 | 39 | 1 | 14 | 81 | 457 | 525 | 5.1e-07 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47797.t1 | G7KVV4 | 86.5 | 96 | 13 | 0 | 1 | 96 | 208 | 303 | 1.2e-41 | 178.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene47797.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47797.t1 | MTR_7g050560 | 86.458 | 96 | 13 | 0 | 1 | 96 | 208 | 303 | 1.38e-56 | 177 |
| MS.gene47797.t1 | MTR_7g050580 | 74.227 | 97 | 24 | 1 | 1 | 96 | 207 | 303 | 2.56e-48 | 155 |
| MS.gene47797.t1 | MTR_7g050590 | 76.531 | 98 | 21 | 2 | 1 | 96 | 196 | 293 | 9.39e-47 | 151 |
| MS.gene47797.t1 | MTR_3g098790 | 71.875 | 96 | 26 | 1 | 2 | 96 | 217 | 312 | 1.97e-44 | 146 |
| MS.gene47797.t1 | MTR_7g050710 | 68.687 | 99 | 28 | 2 | 1 | 96 | 222 | 320 | 6.28e-41 | 137 |
| MS.gene47797.t1 | MTR_7g050720 | 61.111 | 90 | 30 | 5 | 1 | 85 | 228 | 317 | 1.33e-26 | 99.8 |
| MS.gene47797.t1 | MTR_1g108780 | 36.145 | 83 | 45 | 1 | 1 | 83 | 216 | 290 | 1.64e-11 | 58.9 |
| MS.gene47797.t1 | MTR_1g035460 | 40.625 | 96 | 45 | 4 | 2 | 95 | 215 | 300 | 1.93e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATTTGTTGTTAAATGA+AGG | - | 51859:17822-17841 | MS.gene47797:CDS | 15.0% |
| ! | TGTTGTTAAATGAAGGAATT+TGG | - | 51859:17829-17848 | MS.gene47797:CDS | 25.0% |
| AAGGACAATAACTTGAAAGT+TGG | - | 51859:17927-17946 | MS.gene47797:CDS | 30.0% | |
| ATCAAACTGTAGTCCTTTAA+GGG | + | 51859:17880-17899 | MS.gene47797:intergenic | 30.0% | |
| TCTAAAGATGTGAACAATGA+AGG | + | 51859:17994-18013 | MS.gene47797:intergenic | 30.0% | |
| !! | TTTGATTTGGATAAGAAGAG+AGG | - | 51859:17795-17814 | MS.gene47797:CDS | 30.0% |
| GATCAAACTGTAGTCCTTTA+AGG | + | 51859:17881-17900 | MS.gene47797:intergenic | 35.0% | |
| TGGTAAGACACACTTTGATT+TGG | - | 51859:17782-17801 | MS.gene47797:CDS | 35.0% | |
| TGTGTCTTACCAAACTTAAC+TGG | + | 51859:17774-17793 | MS.gene47797:intergenic | 35.0% | |
| AGTTTGATCTCACAACTGGA+TGG | - | 51859:17892-17911 | MS.gene47797:CDS | 40.0% | |
| ATCCTGATTAGGTACTTTGC+AGG | + | 51859:17855-17874 | MS.gene47797:intergenic | 40.0% | |
| CTAATCAGGATGACCCTTAA+AGG | - | 51859:17864-17883 | MS.gene47797:CDS | 40.0% | |
| CTACAGTTTGATCTCACAAC+TGG | - | 51859:17888-17907 | MS.gene47797:CDS | 40.0% | |
| ! | CTTTAAGGGTCATCCTGATT+AGG | + | 51859:17866-17885 | MS.gene47797:intergenic | 40.0% |
| TGATCTCACAACTGGATGGA+AGG | - | 51859:17896-17915 | MS.gene47797:CDS | 45.0% | |
| !! | TGGATGGAAGGCATTTGCAA+AGG | - | 51859:17908-17927 | MS.gene47797:CDS | 45.0% |
| ! | GGCCTGCAAAGTACCTAATC+AGG | - | 51859:17850-17869 | MS.gene47797:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51859 | gene | 17765 | 18053 | 17765 | ID=MS.gene47797 |
| 51859 | mRNA | 17765 | 18053 | 17765 | ID=MS.gene47797.t1;Parent=MS.gene47797 |
| 51859 | exon | 17765 | 18053 | 17765 | ID=MS.gene47797.t1.exon1;Parent=MS.gene47797.t1 |
| 51859 | CDS | 17765 | 18053 | 17765 | ID=cds.MS.gene47797.t1;Parent=MS.gene47797.t1 |
| Gene Sequence |
| Protein sequence |
