AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48069

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48069.t1 MTR_5g060780 70.642 327 43 4 40 323 17 333 3.75e-150 431
MS.gene48069.t1 MTR_5g061890 69.412 340 45 5 40 330 17 346 8.87e-150 430
MS.gene48069.t1 MTR_5g460920 70.336 327 44 4 40 323 17 333 4.61e-148 426
MS.gene48069.t1 MTR_5g061220 42.373 472 138 13 40 397 24 475 4.59e-101 310
MS.gene48069.t1 MTR_1g058210 45.455 418 144 12 39 402 16 403 1.71e-94 291
MS.gene48069.t1 MTR_2g024430 39.913 461 160 17 40 402 25 466 2.00e-79 254
MS.gene48069.t1 MTR_5g060630 45.187 374 114 11 107 402 163 523 2.61e-75 243
MS.gene48069.t1 MTR_5g060630 69.444 72 22 0 41 112 18 89 1.13e-23 103
MS.gene48069.t1 MTR_5g460740 45.187 374 114 11 107 402 163 523 2.61e-75 243
MS.gene48069.t1 MTR_5g460740 69.444 72 22 0 41 112 18 89 1.13e-23 103
MS.gene48069.t1 MTR_5g040880 36.596 470 175 12 40 402 16 469 6.85e-74 239
MS.gene48069.t1 MTR_2g006380 39.628 376 147 16 40 360 25 375 2.14e-64 213
MS.gene48069.t1 MTR_5g082140 40.432 324 125 9 41 308 18 329 8.06e-64 211
MS.gene48069.t1 MTR_5g040740 42.173 313 109 10 40 295 17 314 3.34e-60 201
MS.gene48069.t1 MTR_5g060770 47.015 268 52 7 84 307 7 228 3.07e-57 191
MS.gene48069.t1 MTR_2g006270 38.133 375 161 14 40 360 18 375 1.30e-55 190
MS.gene48069.t1 MTR_5g062960 51.064 188 66 4 207 372 1 184 2.57e-46 158
MS.gene48069.t1 MTR_3g073420 29.352 494 191 18 41 394 25 500 1.96e-42 157
MS.gene48069.t1 MTR_5g460910 37.609 343 101 15 74 348 13 310 2.64e-41 149
MS.gene48069.t1 MTR_2g094570 30.556 360 146 11 40 305 16 365 1.54e-38 147
MS.gene48069.t1 MTR_2g094570 30.556 360 146 11 40 305 16 365 1.57e-38 147
MS.gene48069.t1 MTR_2g094570 30.556 360 146 11 40 305 72 421 1.72e-38 147
MS.gene48069.t1 MTR_1g094960 29.888 358 150 10 40 305 24 372 1.63e-36 140
MS.gene48069.t1 MTR_4g058930 31.694 366 140 11 40 305 17 372 4.67e-35 137
MS.gene48069.t1 MTR_4g058930 31.694 366 140 11 40 305 17 372 5.14e-35 137
MS.gene48069.t1 MTR_7g101280 26.303 403 169 11 41 333 37 421 5.57e-30 122
MS.gene48069.t1 MTR_0041s0110 26.303 403 169 11 41 333 37 421 5.57e-30 122
MS.gene48069.t1 MTR_7g101275 27.476 313 162 6 45 300 2 306 3.94e-27 112
MS.gene48069.t1 MTR_5g062970 72.000 75 18 2 39 113 42 113 4.70e-27 108
MS.gene48069.t1 MTR_8g100050 27.190 331 162 10 40 304 64 381 1.41e-26 112
MS.gene48069.t1 MTR_8g100050 26.554 354 170 11 40 327 64 393 1.64e-26 112
MS.gene48069.t1 MTR_2g018690 28.213 319 163 11 40 298 28 340 2.30e-23 103
MS.gene48069.t1 MTR_1g064430 33.188 229 96 8 127 305 168 389 1.70e-22 100
MS.gene48069.t1 MTR_1g064430 47.945 73 35 1 40 112 32 101 1.34e-13 72.8
MS.gene48069.t1 MTR_1g064430 33.188 229 96 8 127 305 181 402 1.73e-22 100
MS.gene48069.t1 MTR_1g064430 47.945 73 35 1 40 112 45 114 1.38e-13 72.8
MS.gene48069.t1 MTR_5g076270 27.900 319 164 11 40 298 29 341 2.16e-22 100
MS.gene48069.t1 MTR_1g024025 27.114 343 183 12 26 308 34 369 2.34e-22 100
MS.gene48069.t1 MTR_3g064050 26.118 425 217 16 40 395 31 427 7.40e-22 98.6
MS.gene48069.t1 MTR_4g021580 26.513 347 181 11 41 330 25 354 9.05e-22 97.8
MS.gene48069.t1 MTR_4g021580 26.513 347 181 11 41 330 25 354 9.06e-22 97.8
MS.gene48069.t1 MTR_8g079492 27.129 317 168 9 40 298 28 339 1.21e-21 97.8
MS.gene48069.t1 MTR_8g079492 27.129 317 168 9 40 298 28 339 1.32e-21 97.8
MS.gene48069.t1 MTR_4g124900 26.730 318 167 11 40 298 28 338 1.91e-21 97.4
MS.gene48069.t1 MTR_4g124900 26.730 318 167 11 40 298 28 338 2.02e-21 97.4
MS.gene48069.t1 MTR_0041s0120 25.846 325 164 9 45 300 2 318 2.44e-21 95.5
MS.gene48069.t1 MTR_8g027440 25.641 351 180 13 40 330 34 363 7.45e-21 95.1
MS.gene48069.t1 MTR_2g043250 27.273 319 164 13 40 298 28 338 1.82e-20 94.4
MS.gene48069.t1 MTR_2g005240 26.687 326 176 11 40 308 28 347 2.02e-20 94.0
MS.gene48069.t1 MTR_2g014770 24.716 352 187 13 40 330 58 392 4.16e-19 89.7
MS.gene48069.t1 MTR_2g014770 24.716 352 187 13 40 330 58 392 5.04e-19 89.7
MS.gene48069.t1 MTR_4g088210 25.592 211 132 7 128 330 188 381 5.69e-14 73.9
MS.gene48069.t1 MTR_4g088210 25.592 211 132 7 128 330 188 381 6.05e-14 73.9
MS.gene48069.t1 MTR_7g062540 28.910 211 125 8 128 330 156 349 6.39e-14 73.9
MS.gene48069.t1 MTR_8g101360 25.472 318 173 11 40 298 36 348 1.74e-13 72.8
MS.gene48069.t1 MTR_4g060460 29.670 182 109 7 128 298 206 379 2.05e-12 69.3
MS.gene48069.t1 MTR_4g060460 29.670 182 109 7 128 298 206 379 2.14e-12 69.3
MS.gene48069.t1 MTR_8g079492 29.609 179 113 5 128 298 84 257 3.35e-12 68.6
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 221 415 7.78e-12 67.4
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 208 402 9.29e-12 67.4
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 221 415 9.62e-12 67.4
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 208 402 9.97e-12 67.0
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 221 415 1.01e-11 67.4
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 91 285 1.04e-11 67.0
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 208 402 1.13e-11 67.0
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 78 272 1.18e-11 67.0
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 91 285 1.24e-11 67.0
MS.gene48069.t1 MTR_2g093740 28.708 209 128 8 128 329 78 272 1.42e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48069.t1 AT1G77850 30.411 365 155 11 30 305 13 367 2.86e-43 159
MS.gene48069.t1 AT1G77850 30.411 365 155 11 30 305 13 367 3.17e-43 159
MS.gene48069.t1 AT3G61830 28.354 328 164 10 40 307 30 346 2.31e-29 120
MS.gene48069.t1 AT4G30080 29.337 392 173 13 40 338 24 404 4.31e-29 119
MS.gene48069.t1 AT2G46530 28.308 325 168 9 40 307 26 342 4.77e-27 113
MS.gene48069.t1 AT5G37020 29.375 320 158 13 40 298 27 339 3.16e-25 108
MS.gene48069.t1 AT5G37020 29.375 320 158 13 40 298 27 339 3.28e-25 108
MS.gene48069.t1 AT2G46530 28.923 325 166 11 40 307 47 363 2.74e-24 105
MS.gene48069.t1 AT1G59750 26.959 319 170 10 40 301 27 339 4.19e-24 105
MS.gene48069.t1 AT1G59750 26.959 319 170 10 40 301 27 339 4.19e-24 105
MS.gene48069.t1 AT1G59750 26.959 319 170 10 40 301 27 339 4.75e-24 104
MS.gene48069.t1 AT1G59750 26.959 319 170 10 40 301 27 339 4.78e-24 104
MS.gene48069.t1 AT1G30330 28.931 318 162 10 40 298 30 342 1.19e-23 103
MS.gene48069.t1 AT1G30330 28.931 318 162 10 40 298 30 342 1.19e-23 103
MS.gene48069.t1 AT1G30330 28.931 318 162 10 40 298 28 340 1.24e-23 103
MS.gene48069.t1 AT1G34390 26.398 322 154 10 40 298 32 333 1.27e-22 100
MS.gene48069.t1 AT1G34310 27.160 324 151 11 40 298 32 335 2.60e-22 99.4
MS.gene48069.t1 AT1G35540 26.852 324 152 11 40 298 32 335 4.71e-22 98.6
MS.gene48069.t1 AT1G34410 26.543 324 153 11 40 298 32 335 2.51e-21 96.7
MS.gene48069.t1 AT1G35240 27.077 325 150 12 40 298 32 335 3.11e-21 96.3
MS.gene48069.t1 AT1G35520 26.852 324 152 11 40 298 32 335 3.14e-21 96.3
MS.gene48069.t1 AT2G28350 32.287 223 102 8 127 305 154 371 5.81e-21 95.5
MS.gene48069.t1 AT2G28350 32.287 223 102 8 127 305 154 371 8.08e-21 95.1
MS.gene48069.t1 AT1G19850 26.606 327 174 10 40 306 48 368 1.92e-19 91.3
MS.gene48069.t1 AT1G19220 25.625 320 168 11 40 298 28 338 2.03e-19 91.3
MS.gene48069.t1 AT1G19850 26.606 327 174 10 40 306 59 379 2.08e-19 91.3
MS.gene48069.t1 AT5G20730 25.705 319 170 11 40 298 29 340 2.38e-19 90.9
MS.gene48069.t1 AT5G20730 25.705 319 170 11 40 298 29 340 2.71e-19 90.9
MS.gene48069.t1 AT5G20730 26.019 319 168 12 40 298 29 339 6.27e-19 89.7
MS.gene48069.t1 AT2G46530 33.690 187 109 6 128 307 77 255 1.24e-18 88.2
MS.gene48069.t1 AT3G61830 26.531 294 149 9 70 307 13 295 2.93e-18 87.0
MS.gene48069.t1 AT2G33860 30.168 179 111 6 128 298 42 214 2.37e-16 80.9
MS.gene48069.t1 AT2G33860 30.168 179 111 6 128 298 199 371 2.60e-16 81.3
MS.gene48069.t1 AT5G37020 32.222 180 107 8 128 298 71 244 1.28e-14 76.3
MS.gene48069.t1 AT5G62000 26.991 226 123 9 128 334 204 406 2.31e-14 75.5
MS.gene48069.t1 AT5G62000 26.991 226 123 9 128 334 204 406 2.58e-14 75.5
MS.gene48069.t1 AT5G62000 26.991 226 123 9 128 334 204 406 2.58e-14 75.5
MS.gene48069.t1 AT5G62000 26.991 226 123 9 128 334 204 406 2.58e-14 75.5
MS.gene48069.t1 AT5G62000 26.991 226 123 9 128 334 204 406 2.58e-14 75.5
MS.gene48069.t1 AT4G23980 29.947 187 116 7 128 307 156 334 2.85e-14 75.1
MS.gene48069.t1 AT4G23980 29.947 187 116 7 128 307 156 334 3.15e-14 75.1
MS.gene48069.t1 AT5G60450 28.492 179 113 7 128 298 193 364 2.25e-12 69.3
MS.gene48069.t1 AT5G60450 28.492 179 113 7 128 298 217 388 2.41e-12 69.3

Find 110 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5.3:-76789017 MS.gene48069:intron
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.3:+76790979 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.3:-76789788 MS.gene48069:CDS
TTGGCTCATTGGACCTATTA+TGG 0.290349 5.3:+76788544 None:intergenic
GGTTTGGATTCTGTGGGATA+TGG 0.299940 5.3:+76788956 None:intergenic
ACCTCAAGCATGCGCCAATT+AGG 0.307618 5.3:+76789774 None:intergenic
TTTGGATTCTGTGGGATATT+AGG 0.307838 5.3:+76788988 None:intergenic
TCACTTGGTGCTTGGCTCAT+TGG 0.308376 5.3:+76788533 None:intergenic
GGGATATGGTTTGGATGGTT+TGG 0.314165 5.3:+76788970 None:intergenic
TGCCAAGTATTGACTAGGTT+TGG 0.319081 5.3:+76788940 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.321701 5.3:-76790956 MS.gene48069:CDS
CGCCGTTACGCCGTGTATTC+CGG 0.325201 5.3:-76791423 MS.gene48069:intron
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.3:-76789906 MS.gene48069:CDS
CTTGGTCAGTTACGGGAGTT+AGG 0.346501 5.3:+76790800 None:intergenic
CGTTGCCTCATAACCATAAT+AGG 0.348120 5.3:-76788557 MS.gene48069:CDS
ATGGTGATTGTGGCAGCGTT+AGG 0.364809 5.3:+76790916 None:intergenic
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.3:+76789775 None:intergenic
ACAAAAGAGATTGTCCCTTT+AGG 0.373244 5.3:+76789819 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.3:+76790994 None:intergenic
GGATTCTGTGGGATATGGTT+TGG 0.376628 5.3:+76788961 None:intergenic
ATGAATCTGATACAGATAAA+TGG 0.381071 5.3:+76788694 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.3:+76789908 None:intergenic
GATAAGCATGACGGATTCAC+TGG 0.385768 5.3:-76788657 MS.gene48069:CDS
CTCTTTATCATAGGTTAACT+GGG 0.403608 5.3:-76789016 MS.gene48069:intron
GTAATACTCTTGGTCAGTTA+CGG 0.406463 5.3:+76790792 None:intergenic
TAATACTCTTGGTCAGTTAC+GGG 0.414934 5.3:+76790793 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.416879 5.3:+76790076 None:intergenic
TCTGTGGGATATGGTTTGGA+TGG 0.420148 5.3:+76788965 None:intergenic
TTCCTCCTTCATTCCTATGC+AGG 0.431062 5.3:-76788736 MS.gene48069:CDS
TGGTGATTGTGGCAGCGTTA+GGG 0.433150 5.3:+76790917 None:intergenic
TATAAGTGTACCTCCATTCT+CGG 0.441745 5.3:+76790231 None:intergenic
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.3:+76790999 None:intergenic
TCATTCCTATGCAGGGAGTC+AGG 0.445797 5.3:-76788728 MS.gene48069:CDS
TTGGACCTATTATGGTTATG+AGG 0.446632 5.3:+76788552 None:intergenic
TTGGATGGTTTGGATTCTGT+GGG 0.448826 5.3:+76788980 None:intergenic
TACGGGAGTTAGGAGCAGTT+TGG 0.459974 5.3:+76790810 None:intergenic
TTTGGATGGTTTGGATTCTG+TGG 0.470031 5.3:+76788979 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.3:-76789918 MS.gene48069:CDS
AATGAAAACAACAGAATCGT+TGG 0.478070 5.3:+76790142 None:intergenic
ACTGGGAATAATTCAATGGC+AGG 0.481964 5.3:-76788639 MS.gene48069:CDS
CAACCTGCCAAGTATTGACT+AGG 0.486943 5.3:+76788935 None:intergenic
GAAGATTCATCTTTCTTCAA+AGG 0.488166 5.3:+76789867 None:intergenic
CGATGTACATGTAATACTCT+TGG 0.489846 5.3:+76790782 None:intergenic
TGACATATTCTCAGCCTAAA+GGG 0.490948 5.3:-76789833 MS.gene48069:CDS
ATGACATATTCTCAGCCTAA+AGG 0.493756 5.3:-76789834 MS.gene48069:CDS
ATCCAAACCTAGTCAATACT+TGG 0.494257 5.3:-76788942 MS.gene48069:CDS
CAGTTTGGCAAAGACCTCTT+CGG 0.495832 5.3:+76790825 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.500782 5.3:-76789012 MS.gene48069:intron
TTGACTAGGTTTGGATTCTG+TGG 0.502251 5.3:+76788949 None:intergenic
TGACTAGGTTTGGATTCTGT+GGG 0.503890 5.3:+76788950 None:intergenic
TCTGCTGGAAAGATATTTGT+CGG 0.504917 5.3:-76790113 MS.gene48069:CDS
AAGATACCTCACTTGGTGCT+TGG 0.507669 5.3:+76788525 None:intergenic
GACTCCCTGCATAGGAATGA+AGG 0.513722 5.3:+76788731 None:intergenic
AGATATTTGTCGGAATTCGC+AGG 0.516804 5.3:-76790103 MS.gene48069:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.3:-76789775 MS.gene48069:intron
ACAGATGAAGCGGGTAAAGA+AGG 0.527655 5.3:-76790065 MS.gene48069:CDS
TCCTCCTTCATTCCTATGCA+GGG 0.528220 5.3:-76788735 MS.gene48069:CDS
GGCAGAGACGATGCATGGAA+TGG 0.529105 5.3:+76790873 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.532282 5.3:+76790877 None:intergenic
CCAAGGTTTAGCTCTATGTC+AGG 0.539866 5.3:+76788600 None:intergenic
AAGGAGGAAGTTGACGGTGG+AGG 0.544243 5.3:+76788750 None:intergenic
AAACTCAGTAGATTACATAC+TGG 0.547036 5.3:-76790200 MS.gene48069:CDS
CCGACCTTAAAAGCCGAGAA+TGG 0.547731 5.3:-76790244 MS.gene48069:CDS
AAAATATGGTAGAAATGCGC+CGG 0.548670 5.3:-76791018 MS.gene48069:intron
AAACCTAGTCAATACTTGGC+AGG 0.550621 5.3:-76788938 MS.gene48069:intron
AATTCAATGGCAGGGTTGAG+TGG 0.552586 5.3:-76788630 MS.gene48069:CDS
TACACGGCGTAACGGCGGAA+TGG 0.554200 5.3:+76791429 None:intergenic
ATTCACTGGGAATAATTCAA+TGG 0.557749 5.3:-76788643 MS.gene48069:CDS
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.3:-76789914 MS.gene48069:CDS
GTGTATTATCCGACAGCTAG+TGG 0.562344 5.3:-76789972 MS.gene48069:CDS
GCAAAGACCTCTTCGGTGTG+AGG 0.573732 5.3:+76790832 None:intergenic
CATGCTCCAAATGACCTTGT+GGG 0.574839 5.3:+76790950 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.577585 5.3:-76790196 MS.gene48069:CDS
GAATGAGTTCACGAGACAGT+TGG 0.580016 5.3:-76791486 MS.gene48069:CDS
TCATGCCTGACTCCCTGCAT+AGG 0.582427 5.3:+76788723 None:intergenic
CCTGACATAGAGCTAAACCT+TGG 0.585110 5.3:-76788600 MS.gene48069:CDS
TGGACGGTAACTATCAAGAA+TGG 0.587230 5.3:+76790897 None:intergenic
ACTATCAAGAATGGTGATTG+TGG 0.590559 5.3:+76790906 None:intergenic
ATGAAGGAGGAAGTTGACGG+TGG 0.590817 5.3:+76788747 None:intergenic
GGTGATTGTGGCAGCGTTAG+GGG 0.592113 5.3:+76790918 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.598930 5.3:-76790075 MS.gene48069:CDS
TGTTGATCCTCACACCGAAG+AGG 0.601436 5.3:-76790839 MS.gene48069:CDS
TCAACGGCAGAGACGATGCA+TGG 0.604313 5.3:+76790868 None:intergenic
GCCAATGAAGATACCTCACT+TGG 0.608165 5.3:+76788518 None:intergenic
CTGGGAATAATTCAATGGCA+GGG 0.616068 5.3:-76788638 MS.gene48069:CDS
GCAGAGCCTACCGGAATACA+CGG 0.616741 5.3:+76791413 None:intergenic
ATTATCCGACAGCTAGTGGT+TGG 0.618045 5.3:-76789968 MS.gene48069:CDS
CGATGCATGGAATGGATGGA+CGG 0.620709 5.3:+76790881 None:intergenic
GCATGCTCCAAATGACCTTG+TGG 0.621993 5.3:+76790949 None:intergenic
CTAAACCTTGGCAGTCCTAG+AGG 0.624031 5.3:-76788588 MS.gene48069:CDS
TACCGGAATACACGGCGTAA+CGG 0.624639 5.3:+76791421 None:intergenic
GATGAAGCGGGTAAAGAAGG+AGG 0.625313 5.3:-76790062 MS.gene48069:CDS
GGAATGAAGGAGGAAGTTGA+CGG 0.626658 5.3:+76788744 None:intergenic
TAATCTACTGAGTTTCGCGT+AGG 0.626849 5.3:+76790208 None:intergenic
CAGCACCTCTAGGACTGCCA+AGG 0.629336 5.3:+76788583 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.3:+76790993 None:intergenic
ATAAGCATGACGGATTCACT+GGG 0.631573 5.3:-76788656 MS.gene48069:CDS
AAGTTCACTACAGATGAAGC+GGG 0.636021 5.3:-76790074 MS.gene48069:CDS
GGCAACGAGTCAGCACCTCT+AGG 0.637822 5.3:+76788573 None:intergenic
ACCTTAAAAGCCGAGAATGG+AGG 0.638927 5.3:-76790241 MS.gene48069:CDS
TTTAGATGCAGCGGAACTGG+CGG 0.641845 5.3:-76790018 MS.gene48069:CDS
ATGAAGCGGGTAAAGAAGGA+GGG 0.650423 5.3:-76790061 MS.gene48069:CDS
TCACGCCAACCACTAGCTGT+CGG 0.652491 5.3:+76789963 None:intergenic
AATGAGCCAAGCACCAAGTG+AGG 0.655123 5.3:-76788531 None:intergenic
AGGATCAACAAAGAGATCAA+CGG 0.658354 5.3:+76790852 None:intergenic
CGGAATACACGGCGTAACGG+CGG 0.659535 5.3:+76791424 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.3:+76790995 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.678948 5.3:-76790964 MS.gene48069:CDS
TCCCTGCATAGGAATGAAGG+AGG 0.687917 5.3:+76788734 None:intergenic
ACGTATAGCGAAGAAATGCA+CGG 0.718371 5.3:-76791510 MS.gene48069:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATCAAATAATCTTATTA+TGG + chr5.3:76790380-76790399 None:intergenic 15.0%
!!! ATTTTTTATCTCTTTATCAT+AGG - chr5.3:76791023-76791042 MS.gene48069:intron 15.0%
!!! CTTAATTTTCAAATTACATT+TGG + chr5.3:76790495-76790514 None:intergenic 15.0%
!! ATAGCTCACTATATTTATTT+AGG - chr5.3:76789631-76789650 MS.gene48069:intron 20.0%
!! CTAAAATTATGAAACTTAGT+AGG - chr5.3:76790442-76790461 MS.gene48069:intron 20.0%
!! TCTTATTATGGATTCAAATT+TGG + chr5.3:76790368-76790387 None:intergenic 20.0%
! ATAAGAATTTGGTACGTATA+GGG + chr5.3:76789699-76789718 None:intergenic 25.0%
! ATGAATCTGATACAGATAAA+TGG + chr5.3:76791357-76791376 None:intergenic 25.0%
! CACATAATTCTTGTAATCAA+GGG - chr5.3:76789365-76789384 MS.gene48069:intron 25.0%
! CTCATGTTAACTTAAAAACT+TGG - chr5.3:76788821-76788840 MS.gene48069:intron 25.0%
! GGAATTATTAATGTTAGAGA+TGG + chr5.3:76790542-76790561 None:intergenic 25.0%
! TAGGGAGTTACATATAAAAA+CGG + chr5.3:76790656-76790675 None:intergenic 25.0%
! TCACATAATTCTTGTAATCA+AGG - chr5.3:76789364-76789383 MS.gene48069:intron 25.0%
! TTACAAGAATTATGTGACTA+TGG + chr5.3:76789361-76789380 None:intergenic 25.0%
!! ATGGATTAAGCTTATAATTG+AGG + chr5.3:76788856-76788875 None:intergenic 25.0%
!!! GTACTTATGCTATCTTATTT+TGG - chr5.3:76790295-76790314 MS.gene48069:intron 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5.3:76789072-76789091 None:intergenic 25.0%
AAACTCAGTAGATTACATAC+TGG - chr5.3:76789848-76789867 MS.gene48069:CDS 30.0%
AATGAAAACAACAGAATCGT+TGG + chr5.3:76789909-76789928 None:intergenic 30.0%
AGGGAGTTACATATAAAAAC+GGG + chr5.3:76790655-76790674 None:intergenic 30.0%
ATTCACTGGGAATAATTCAA+TGG - chr5.3:76791405-76791424 MS.gene48069:intron 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5.3:76790260-76790279 MS.gene48069:CDS 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr5.3:76791032-76791051 MS.gene48069:intron 30.0%
GAAACTTGGTAGAAGAAATT+AGG - chr5.3:76789588-76789607 MS.gene48069:intron 30.0%
GAAGATTCATCTTTCTTCAA+AGG + chr5.3:76790184-76790203 None:intergenic 30.0%
GATAAGAATTTGGTACGTAT+AGG + chr5.3:76789700-76789719 None:intergenic 30.0%
GCTTATAATTGAGGTAATCT+TGG + chr5.3:76788847-76788866 None:intergenic 30.0%
GTATTATTACTACTACGAAC+CGG - chr5.3:76789653-76789672 MS.gene48069:intron 30.0%
TAAGAATTTGGTACGTATAG+GGG + chr5.3:76789698-76789717 None:intergenic 30.0%
TACAATGAGAAATGCTTAGA+AGG + chr5.3:76790733-76790752 None:intergenic 30.0%
TCAAGATTCTACTTAGCATT+AGG + chr5.3:76790762-76790781 None:intergenic 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr5.3:76791031-76791050 MS.gene48069:intron 30.0%
TGTAATTTAGGCAGTGTAAT+TGG + chr5.3:76791277-76791296 None:intergenic 30.0%
TTAGAGAATGTGGAATTTGT+TGG + chr5.3:76791252-76791271 None:intergenic 30.0%
TTCAGTTATAGATGATTGCA+TGG + chr5.3:76788875-76788894 None:intergenic 30.0%
! GCTAAGTAGAATCTTGATTT+TGG - chr5.3:76790765-76790784 MS.gene48069:CDS 30.0%
! GTTTTCATTAAAAACTCTGC+TGG - chr5.3:76789920-76789939 MS.gene48069:CDS 30.0%
! TAGAGAATGTGGAATTTGTT+GGG + chr5.3:76791251-76791270 None:intergenic 30.0%
!! AAAGTCTAGGACTTTTTGTT+GGG + chr5.3:76791213-76791232 None:intergenic 30.0%
!! GTTTTGAATGATAAGCATGA+CGG - chr5.3:76791382-76791401 MS.gene48069:intron 30.0%
!!! CTTTTTTTTCAACCAATCAG+CGG + chr5.3:76789484-76789503 None:intergenic 30.0%
AAAATATGGTAGAAATGCGC+CGG - chr5.3:76789030-76789049 MS.gene48069:intron 35.0%
AAAGTTTACTACTTCCCACA+AGG - chr5.3:76789084-76789103 MS.gene48069:intron 35.0%
AAATGAATCACCATGAGTTG+AGG - chr5.3:76789527-76789546 MS.gene48069:intron 35.0%
ACAAAAGAGATTGTCCCTTT+AGG + chr5.3:76790232-76790251 None:intergenic 35.0%
ACTATCAAGAATGGTGATTG+TGG + chr5.3:76789145-76789164 None:intergenic 35.0%
AGATTTCCAACCAATCTTCA+TGG + chr5.3:76790143-76790162 None:intergenic 35.0%
AGGATCAACAAAGAGATCAA+CGG + chr5.3:76789199-76789218 None:intergenic 35.0%
ATCCAAACCTAGTCAATACT+TGG - chr5.3:76791106-76791125 MS.gene48069:intron 35.0%
ATCTATCAAGTGCAAGAACA+AGG + chr5.3:76790564-76790583 None:intergenic 35.0%
ATGACATATTCTCAGCCTAA+AGG - chr5.3:76790214-76790233 MS.gene48069:CDS 35.0%
CGATGTACATGTAATACTCT+TGG + chr5.3:76789269-76789288 None:intergenic 35.0%
CTAAATTAGCCCACCTAAAT+TGG - chr5.3:76790808-76790827 MS.gene48069:CDS 35.0%
CTATTGCAGGGAGTAATATT+GGG + chr5.3:76788970-76788989 None:intergenic 35.0%
GTAATACTCTTGGTCAGTTA+CGG + chr5.3:76789259-76789278 None:intergenic 35.0%
GTAATTGGCATTAGAGAATG+TGG + chr5.3:76791262-76791281 None:intergenic 35.0%
TAATACTCTTGGTCAGTTAC+GGG + chr5.3:76789258-76789277 None:intergenic 35.0%
TACAAGCTTAGCAAACGAAA+AGG + chr5.3:76788660-76788679 None:intergenic 35.0%
TATAAGTGTACCTCCATTCT+CGG + chr5.3:76789820-76789839 None:intergenic 35.0%
TCTATCAAGTGCAAGAACAA+GGG + chr5.3:76790563-76790582 None:intergenic 35.0%
TCTGCTGGAAAGATATTTGT+CGG - chr5.3:76789935-76789954 MS.gene48069:CDS 35.0%
TGACATATTCTCAGCCTAAA+GGG - chr5.3:76790215-76790234 MS.gene48069:CDS 35.0%
TTATCATAGGTTAACTGGGA+TGG - chr5.3:76791036-76791055 MS.gene48069:intron 35.0%
TTGGACCTATTATGGTTATG+AGG + chr5.3:76791499-76791518 None:intergenic 35.0%
TTTGGATTCTGTGGGATATT+AGG + chr5.3:76791063-76791082 None:intergenic 35.0%
! ACATTTGGAACTTATCCTAC+AGG + chr5.3:76790480-76790499 None:intergenic 35.0%
! AGACAGTTGGCACATTTTAA+TGG - chr5.3:76788575-76788594 MS.gene48069:CDS 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - chr5.3:76790142-76790161 MS.gene48069:CDS 35.0%
! CATTTGGAACTTATCCTACA+GGG + chr5.3:76790479-76790498 None:intergenic 35.0%
! CGCATTTCTACCATATTTTG+GGG + chr5.3:76789029-76789048 None:intergenic 35.0%
! CGCTGATTGGTTGAAAAAAA+AGG - chr5.3:76789482-76789501 MS.gene48069:intron 35.0%
! GCGCATTTCTACCATATTTT+GGG + chr5.3:76789030-76789049 None:intergenic 35.0%
!! AAGTCTAGGACTTTTTGTTG+GGG + chr5.3:76791212-76791231 None:intergenic 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5.3:76789058-76789077 None:intergenic 35.0%
!! AGAGAATGTGGAATTTGTTG+GGG + chr5.3:76791250-76791269 None:intergenic 35.0%
!! GAAAGTCTAGGACTTTTTGT+TGG + chr5.3:76791214-76791233 None:intergenic 35.0%
!! TTGGCACATTTTAATGGCTT+TGG - chr5.3:76788581-76788600 MS.gene48069:CDS 35.0%
!!! GAAGTTGATTTTTTGTTGGG+GGG + chr5.3:76791188-76791207 None:intergenic 35.0%
!!! GGAAGTTGATTTTTTGTTGG+GGG + chr5.3:76791189-76791208 None:intergenic 35.0%
!!! GGGAAGTTGATTTTTTGTTG+GGG + chr5.3:76791190-76791209 None:intergenic 35.0%
!!! GGGGAAGTTGATTTTTTGTT+GGG + chr5.3:76791191-76791210 None:intergenic 35.0%
AAACCTAGTCAATACTTGGC+AGG - chr5.3:76791110-76791129 MS.gene48069:intron 40.0%
AAGTTCACTACAGATGAAGC+GGG - chr5.3:76789974-76789993 MS.gene48069:CDS 40.0%
ACGTATAGCGAAGAAATGCA+CGG - chr5.3:76788538-76788557 MS.gene48069:CDS 40.0%
ACTATTGCAGCAAGTCAAGT+AGG + chr5.3:76790675-76790694 None:intergenic 40.0%
ACTGGGAATAATTCAATGGC+AGG - chr5.3:76791409-76791428 MS.gene48069:intron 40.0%
AGAAGAACATGAACGAAGAG+TGG - chr5.3:76789558-76789577 MS.gene48069:intron 40.0%
AGAGTGGAGAATGAGAAACT+TGG - chr5.3:76789574-76789593 MS.gene48069:intron 40.0%
AGATATTTGTCGGAATTCGC+AGG - chr5.3:76789945-76789964 MS.gene48069:CDS 40.0%
ATCAAGGGAGAATTGCGAAT+TGG - chr5.3:76789380-76789399 MS.gene48069:intron 40.0%
ATCTCATGTTCGATTCTCTC+CGG + chr5.3:76790836-76790855 None:intergenic 40.0%
CAAGTTCACTACAGATGAAG+CGG - chr5.3:76789973-76789992 MS.gene48069:CDS 40.0%
CGTTGCCTCATAACCATAAT+AGG - chr5.3:76791491-76791510 MS.gene48069:CDS 40.0%
CTATTGCAGCAAGTCAAGTA+GGG + chr5.3:76790674-76790693 None:intergenic 40.0%
CTGGGAATAATTCAATGGCA+GGG - chr5.3:76791410-76791429 MS.gene48069:intron 40.0%
GAATCGAACATGAGATCTCA+AGG - chr5.3:76790840-76790859 MS.gene48069:CDS 40.0%
GAGCATAGTCACATAATTCG+CGG + chr5.3:76789769-76789788 None:intergenic 40.0%
GCTATTGCAGGGAGTAATAT+TGG + chr5.3:76788971-76788990 None:intergenic 40.0%
GTATTCATGATGCTCTCATG+AGG - chr5.3:76788677-76788696 MS.gene48069:CDS 40.0%
TAATCTACTGAGTTTCGCGT+AGG + chr5.3:76789843-76789862 None:intergenic 40.0%
TACGCGACAGGATAAGAATT+TGG + chr5.3:76789710-76789729 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG - chr5.3:76789852-76789871 MS.gene48069:CDS 40.0%
TGACTAGGTTTGGATTCTGT+GGG + chr5.3:76791101-76791120 None:intergenic 40.0%
TGCCAAAATCAAACCCACTT+GGG + chr5.3:76790907-76790926 None:intergenic 40.0%
TGGACGGTAACTATCAAGAA+TGG + chr5.3:76789154-76789173 None:intergenic 40.0%
TTGACTAGGTTTGGATTCTG+TGG + chr5.3:76791102-76791121 None:intergenic 40.0%
TTGGCTCATTGGACCTATTA+TGG + chr5.3:76791507-76791526 None:intergenic 40.0%
! GAAGAACTCTGAGAAAGTCT+AGG + chr5.3:76791226-76791245 None:intergenic 40.0%
! GGCGCATTTCTACCATATTT+TGG + chr5.3:76789031-76789050 None:intergenic 40.0%
! TTGGATGGTTTGGATTCTGT+GGG + chr5.3:76791071-76791090 None:intergenic 40.0%
! TTTGGATGGTTTGGATTCTG+TGG + chr5.3:76791072-76791091 None:intergenic 40.0%
! TTTTGTTGGGGGGAAATTGA+TGG + chr5.3:76791178-76791197 None:intergenic 40.0%
!! AACCCAAGTGGGTTTGATTT+TGG - chr5.3:76790902-76790921 MS.gene48069:CDS 40.0%
!! AGAATGTGGAATTTGTTGGG+GGG + chr5.3:76791248-76791267 None:intergenic 40.0%
!! AGTCTAGGACTTTTTGTTGG+GGG + chr5.3:76791211-76791230 None:intergenic 40.0%
!! ATAAGCATGACGGATTCACT+GGG - chr5.3:76791392-76791411 MS.gene48069:intron 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5.3:76789057-76789076 None:intergenic 40.0%
!! GAGAATGTGGAATTTGTTGG+GGG + chr5.3:76791249-76791268 None:intergenic 40.0%
!! TGCCAAGTATTGACTAGGTT+TGG + chr5.3:76791111-76791130 None:intergenic 40.0%
!!! GATTGCAGTTTTAGATGCAG+CGG - chr5.3:76790021-76790040 MS.gene48069:CDS 40.0%
!!! GGGGGAAGTTGATTTTTTGT+TGG + chr5.3:76791192-76791211 None:intergenic 40.0%
AAAGCATGGTTACCGCTGAT+TGG - chr5.3:76789469-76789488 MS.gene48069:intron 45.0%
AATCAGCGGTAACCATGCTT+TGG + chr5.3:76789470-76789489 None:intergenic 45.0%
AATTCAATGGCAGGGTTGAG+TGG - chr5.3:76791418-76791437 MS.gene48069:intron 45.0%
ACAGATGAAGCGGGTAAAGA+AGG - chr5.3:76789983-76790002 MS.gene48069:CDS 45.0%
ACATAATTCGCGGTTCGTTC+CGG + chr5.3:76789759-76789778 None:intergenic 45.0%
ACCTTAAAAGCCGAGAATGG+AGG - chr5.3:76789807-76789826 MS.gene48069:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG - chr5.3:76790134-76790153 MS.gene48069:CDS 45.0%
AGACTCGTCCAAATCAAGCT+TGG + chr5.3:76789333-76789352 None:intergenic 45.0%
ATGAAGCGGGTAAAGAAGGA+GGG - chr5.3:76789987-76790006 MS.gene48069:CDS 45.0%
ATTATCCGACAGCTAGTGGT+TGG - chr5.3:76790080-76790099 MS.gene48069:CDS 45.0%
CAGTTTGGCAAAGACCTCTT+CGG + chr5.3:76789226-76789245 None:intergenic 45.0%
CATGCTCCAAATGACCTTGT+GGG + chr5.3:76789101-76789120 None:intergenic 45.0%
CCTGACATAGAGCTAAACCT+TGG - chr5.3:76791448-76791467 MS.gene48069:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG + chr5.3:76789975-76789994 None:intergenic 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5.3:76789092-76789111 MS.gene48069:intron 45.0%
CTTCTGTGGACCCCAAAATA+TGG - chr5.3:76789016-76789035 MS.gene48069:intron 45.0%
GAATGAGTTCACGAGACAGT+TGG - chr5.3:76788562-76788581 MS.gene48069:CDS 45.0%
GACTCGTCCAAATCAAGCTT+GGG + chr5.3:76789332-76789351 None:intergenic 45.0%
GATCTTGCCCAAGCTTGATT+TGG - chr5.3:76789322-76789341 MS.gene48069:intron 45.0%
GCAGGGAGTAATATTGGGTT+GGG + chr5.3:76788965-76788984 None:intergenic 45.0%
GCATTGCTATATTTCGCGAG+AGG + chr5.3:76788706-76788725 None:intergenic 45.0%
GGAATGAAGGAGGAAGTTGA+CGG + chr5.3:76791307-76791326 None:intergenic 45.0%
GGATTCTGTGGGATATGGTT+TGG + chr5.3:76791090-76791109 None:intergenic 45.0%
GGTTTGGATTCTGTGGGATA+TGG + chr5.3:76791065-76791084 None:intergenic 45.0%
GTGCCAAAATCAAACCCACT+TGG + chr5.3:76790908-76790927 None:intergenic 45.0%
GTGTATTATCCGACAGCTAG+TGG - chr5.3:76790076-76790095 MS.gene48069:CDS 45.0%
GTTGACGATGCCATGAAGAT+TGG - chr5.3:76790130-76790149 MS.gene48069:CDS 45.0%
TAACCATGCTTTGGTACGCT+TGG + chr5.3:76789461-76789480 None:intergenic 45.0%
TCCTCCTTCATTCCTATGCA+GGG - chr5.3:76791313-76791332 MS.gene48069:intron 45.0%
TCGAACATGAGATCTCAAGG+AGG - chr5.3:76790843-76790862 MS.gene48069:CDS 45.0%
TGCAGGGAGTAATATTGGGT+TGG + chr5.3:76788966-76788985 None:intergenic 45.0%
TTCCTCCTTCATTCCTATGC+AGG - chr5.3:76791312-76791331 MS.gene48069:intron 45.0%
! ACCTCCATTCTCGGCTTTTA+AGG + chr5.3:76789811-76789830 None:intergenic 45.0%
! AGTACCGAATTCGGGTACTT+CGG - chr5.3:76789409-76789428 MS.gene48069:intron 45.0%
! ATTCTCTCCGGTGCCAATTT+AGG + chr5.3:76790824-76790843 None:intergenic 45.0%
! CGTATAGGGGTATACAGCTT+CGG + chr5.3:76789685-76789704 None:intergenic 45.0%
! GGGATATGGTTTGGATGGTT+TGG + chr5.3:76791081-76791100 None:intergenic 45.0%
! TAGTGGTTGGCGTGATTTTG+TGG - chr5.3:76790093-76790112 MS.gene48069:CDS 45.0%
! TCTGTGGGATATGGTTTGGA+TGG + chr5.3:76791086-76791105 None:intergenic 45.0%
!! CAACCTGCCAAGTATTGACT+AGG + chr5.3:76791116-76791135 None:intergenic 45.0%
!! CCAAGGTTTAGCTCTATGTC+AGG + chr5.3:76791451-76791470 None:intergenic 45.0%
!! GATAAGCATGACGGATTCAC+TGG - chr5.3:76791391-76791410 MS.gene48069:intron 45.0%
!! GTCTAGGACTTTTTGTTGGG+GGG + chr5.3:76791210-76791229 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG + chr5.3:76789056-76789075 None:intergenic 45.0%
!! TGGTACGTGAGTACCGAATT+CGG - chr5.3:76789400-76789419 MS.gene48069:intron 45.0%
!! TTGCCTAGTGGTATTGGCTT+GGG + chr5.3:76790884-76790903 None:intergenic 45.0%
!!! AGTTTTAGATGCAGCGGAAC+TGG - chr5.3:76790027-76790046 MS.gene48069:CDS 45.0%
!!! CATATTTTGGGGTCCACAGA+AGG + chr5.3:76789018-76789037 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG + chr5.3:76789807-76789826 None:intergenic 45.0%
AACGAAAAGGCAGAGCCTAC+CGG + chr5.3:76788647-76788666 None:intergenic 50.0%
ACCTCAAGCATGCGCCAATT+AGG + chr5.3:76790277-76790296 None:intergenic 50.0%
ATACCACTAGGCAACCCAAG+TGG - chr5.3:76790890-76790909 MS.gene48069:CDS 50.0%
ATGAAGGAGGAAGTTGACGG+TGG + chr5.3:76791304-76791323 None:intergenic 50.0%
ATGGTGATTGTGGCAGCGTT+AGG + chr5.3:76789135-76789154 None:intergenic 50.0%
ATTCGGGTACTTCGGCGTAT+TGG - chr5.3:76789417-76789436 MS.gene48069:intron 50.0%
CCGACCTTAAAAGCCGAGAA+TGG - chr5.3:76789804-76789823 MS.gene48069:CDS 50.0%
CCTCAAGCATGCGCCAATTA+GGG + chr5.3:76790276-76790295 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5.3:76789170-76789189 None:intergenic 50.0%
CTAAACCTTGGCAGTCCTAG+AGG - chr5.3:76791460-76791479 MS.gene48069:CDS 50.0%
CTCACTCAGTCCTCAACTCA+TGG + chr5.3:76789540-76789559 None:intergenic 50.0%
CTTGGTCAGTTACGGGAGTT+AGG + chr5.3:76789251-76789270 None:intergenic 50.0%
GAACCAAGCGTACCAAAGCA+TGG - chr5.3:76789455-76789474 MS.gene48069:intron 50.0%
GACGGTGGAGGCTGTAATTT+AGG + chr5.3:76791289-76791308 None:intergenic 50.0%
GACTCCCTGCATAGGAATGA+AGG + chr5.3:76791320-76791339 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5.3:76789174-76789193 None:intergenic 50.0%
GATCCCAAGCCAATACCACT+AGG - chr5.3:76790878-76790897 MS.gene48069:CDS 50.0%
GATGAAGCGGGTAAAGAAGG+AGG - chr5.3:76789986-76790005 MS.gene48069:CDS 50.0%
GCATGCTCCAAATGACCTTG+TGG + chr5.3:76789102-76789121 None:intergenic 50.0%
TACCACTAGGCAACCCAAGT+GGG - chr5.3:76790891-76790910 MS.gene48069:CDS 50.0%
TACCGGAATACACGGCGTAA+CGG + chr5.3:76788630-76788649 None:intergenic 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + chr5.3:76789241-76789260 None:intergenic 50.0%
TAGCCCACCTAAATTGGCAC+CGG - chr5.3:76790814-76790833 MS.gene48069:CDS 50.0%
TATACAGCTTCGGTACGCTC+CGG + chr5.3:76789675-76789694 None:intergenic 50.0%
TCATTCCTATGCAGGGAGTC+AGG - chr5.3:76791320-76791339 MS.gene48069:intron 50.0%
TCCCTGCATAGGAATGAAGG+AGG + chr5.3:76791317-76791336 None:intergenic 50.0%
TGGTGATTGTGGCAGCGTTA+GGG + chr5.3:76789134-76789153 None:intergenic 50.0%
TGTTGATCCTCACACCGAAG+AGG - chr5.3:76789209-76789228 MS.gene48069:intron 50.0%
TGTTGGGGGGAAATTGATGG+TGG + chr5.3:76791175-76791194 None:intergenic 50.0%
TTTAGATGCAGCGGAACTGG+CGG - chr5.3:76790030-76790049 MS.gene48069:CDS 50.0%
! AGACATGGTGGAGCTATTGC+AGG + chr5.3:76788983-76789002 None:intergenic 50.0%
! CTTGGGTTGCCTAGTGGTAT+TGG + chr5.3:76790890-76790909 None:intergenic 50.0%
! GACATGGTGGAGCTATTGCA+GGG + chr5.3:76788982-76789001 None:intergenic 50.0%
! TACGCCGAAGTACCCGAATT+CGG + chr5.3:76789416-76789435 None:intergenic 50.0%
! TCTCCGGTGCCAATTTAGGT+GGG + chr5.3:76790820-76790839 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTGG+TGG - chr5.3:76790096-76790115 MS.gene48069:CDS 50.0%
!! AGTGGTATTGGCTTGGGATC+TGG + chr5.3:76790878-76790897 None:intergenic 50.0%
!! GGTACGTGAGTACCGAATTC+GGG - chr5.3:76789401-76789420 MS.gene48069:intron 50.0%
!! GTGGTATTGGCTTGGGATCT+GGG + chr5.3:76790877-76790896 None:intergenic 50.0%
!! GTTGCCTAGTGGTATTGGCT+TGG + chr5.3:76790885-76790904 None:intergenic 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG + chr5.3:76791518-76791537 None:intergenic 50.0%
!!! ATTTTGGGGTCCACAGAAGG+TGG + chr5.3:76789015-76789034 None:intergenic 50.0%
AAGGAGGAAGTTGACGGTGG+AGG + chr5.3:76791301-76791320 None:intergenic 55.0%
AGGTGGCTGAGGAAGAGACA+TGG + chr5.3:76788998-76789017 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG - chr5.3:76790273-76790292 MS.gene48069:intron 55.0%
GCAGAGCCTACCGGAATACA+CGG + chr5.3:76788638-76788657 None:intergenic 55.0%
GGCAGAGACGATGCATGGAA+TGG + chr5.3:76789178-76789197 None:intergenic 55.0%
GGTGATTGTGGCAGCGTTAG+GGG + chr5.3:76789133-76789152 None:intergenic 55.0%
GTTACGCCGTGTATTCCGGT+AGG - chr5.3:76788629-76788648 MS.gene48069:CDS 55.0%
TCAACGGCAGAGACGATGCA+TGG + chr5.3:76789183-76789202 None:intergenic 55.0%
TCACGCCAACCACTAGCTGT+CGG + chr5.3:76790088-76790107 None:intergenic 55.0%
TCATGCCTGACTCCCTGCAT+AGG + chr5.3:76791328-76791347 None:intergenic 55.0%
TCTTCCTCAGCCACCTTCTG+TGG - chr5.3:76789002-76789021 MS.gene48069:CDS 55.0%
TGGCTGAGGAAGAGACATGG+TGG + chr5.3:76788995-76789014 None:intergenic 55.0%
! AACCCACTTGGGTTGCCTAG+TGG + chr5.3:76790896-76790915 None:intergenic 55.0%
! ATGGTGGAGGAGGAAGTCGA+AGG + chr5.3:76791159-76791178 None:intergenic 55.0%
! CTCTCCGGTGCCAATTTAGG+TGG + chr5.3:76790821-76790840 None:intergenic 55.0%
! CTTTGGTACGCTTGGTTCGC+TGG + chr5.3:76789453-76789472 None:intergenic 55.0%
! GCAAAGACCTCTTCGGTGTG+AGG + chr5.3:76789219-76789238 None:intergenic 55.0%
!! AGGCTAGCTTTGTGCCCTGT+AGG - chr5.3:76790462-76790481 MS.gene48069:intron 55.0%
!! GGGGAAATTGATGGTGGAGG+AGG + chr5.3:76791169-76791188 None:intergenic 55.0%
!! TGGGGGGAAATTGATGGTGG+AGG + chr5.3:76791172-76791191 None:intergenic 55.0%
CGCCGTTACGCCGTGTATTC+CGG - chr5.3:76788625-76788644 MS.gene48069:CDS 60.0%
CGGAATACACGGCGTAACGG+CGG + chr5.3:76788627-76788646 None:intergenic 60.0%
GGCAACGAGTCAGCACCTCT+AGG + chr5.3:76791478-76791497 None:intergenic 60.0%
GTTCGTTCCGGTACGCGGTA+CGG + chr5.3:76789747-76789766 None:intergenic 60.0%
TACACGGCGTAACGGCGGAA+TGG + chr5.3:76788622-76788641 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG + chr5.3:76789052-76789071 None:intergenic 60.0%
TTCGTTCCGGTACGCGGTAC+GGG + chr5.3:76789746-76789765 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG + chr5.3:76791153-76791172 None:intergenic 60.0%
!! CAGCACCTCTAGGACTGCCA+AGG + chr5.3:76791468-76791487 None:intergenic 60.0%
GGGTCCACAGAAGGTGGCTG+AGG + chr5.3:76789009-76789028 None:intergenic 65.0%
TCCGGTACGCGGTACGGGTA+CGG + chr5.3:76789741-76789760 None:intergenic 65.0%
TCGCGGTTCGTTCCGGTACG+CGG + chr5.3:76789752-76789771 None:intergenic 65.0%
ACGGGTTCGCGGTACGCGAC+AGG + chr5.3:76789722-76789741 None:intergenic 70.0%
CCCGTACCCGTACCGCGTAC+CGG - chr5.3:76789737-76789756 MS.gene48069:intron 70.0%
CCGGTACGCGGTACGGGTAC+GGG + chr5.3:76789740-76789759 None:intergenic 70.0%
GCGGTACGGGTACGGGTTCG+CGG + chr5.3:76789733-76789752 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 76788533 76791537 76788533 ID=MS.gene48069
chr5.3 mRNA 76788533 76791537 76788533 ID=MS.gene48069.t1;Parent=MS.gene48069
chr5.3 exon 76791424 76791537 76791424 ID=MS.gene48069.t1.exon1;Parent=MS.gene48069.t1
chr5.3 CDS 76791424 76791537 76791424 ID=cds.MS.gene48069.t1;Parent=MS.gene48069.t1
chr5.3 exon 76790765 76791028 76790765 ID=MS.gene48069.t1.exon2;Parent=MS.gene48069.t1
chr5.3 CDS 76790765 76791028 76790765 ID=cds.MS.gene48069.t1;Parent=MS.gene48069.t1
chr5.3 exon 76789776 76790279 76789776 ID=MS.gene48069.t1.exon3;Parent=MS.gene48069.t1
chr5.3 CDS 76789776 76790279 76789776 ID=cds.MS.gene48069.t1;Parent=MS.gene48069.t1
chr5.3 exon 76788939 76789025 76788939 ID=MS.gene48069.t1.exon4;Parent=MS.gene48069.t1
chr5.3 CDS 76788939 76789025 76788939 ID=cds.MS.gene48069.t1;Parent=MS.gene48069.t1
chr5.3 exon 76788533 76788772 76788533 ID=MS.gene48069.t1.exon5;Parent=MS.gene48069.t1
chr5.3 CDS 76788533 76788772 76788533 ID=cds.MS.gene48069.t1;Parent=MS.gene48069.t1
Gene Sequence

>MS.gene48069

ATGCTACGTATAGCGAAGAAATGCACGGCGAATGAGTTCACGAGACAGTTGGCACATTTTAATGGCTTTGGCTTCTGTGTTTTTCGCCATTCCGCCGTTACGCCGTGTATTCCGGTAGGCTCTGCCTTTTCGTTTGCTAAGCTTGTATTCATGATGCTCTCATGAGGATTCCTCTCGCGAAATATAGCAATGCTCTATCATTAAAGAAGTTCAAATATGTCAATTTAAACATGCTTATGATACACATTTATAAGCTTAAGATTACTTTCATACATGATAACTAACAAACTCATGTTAACTTAAAAACTTGGCCAAGATTACCTCAATTATAAGCTTAATCCATGCAATCATCTATAACTGAAACACTACAAATAAACTTAAAACATTTTGTAACTAACTACTTCTCGCTCTTTTTTTCACTATCATACACCCAACCCAATATTACTCCCTGCAATAGCTCCACCATGTCTCTTCCTCAGCCACCTTCTGTGGACCCCAAAATATGGTAGAAATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAGCATGCATGTTCTTCCCCTAACGCTGCCACAATCACCATTCTTGATAGTTACCGTCCATCCATTCCATGCATCGTCTCTGCCGTTGATCTCTTTGTTGATCCTCACACCGAAGAGGTCTTTGCCAAACTGCTCCTAACTCCCGTAACTGACCAAGAGTATTACATGTACATCGAGTATTACATGTACATCGTGAATGCTCTGACTTGATCTTGCCCAAGCTTGATTTGGACGAGTCTCAGAGCCATAGTCACATAATTCTTGTAATCAAGGGAGAATTGCGAATTGGTACGTGAGTACCGAATTCGGGTACTTCGGCGTATTGGTACGCACAAACCAGCGAACCAAGCGTACCAAAGCATGGTTACCGCTGATTGGTTGAAAAAAAAGGTTTAGACTACAAAGTAAATATAAAATGAATCACCATGAGTTGAGGACTGAGTGAGAAGAACATGAACGAAGAGTGGAGAATGAGAAACTTGGTAGAAGAAATTAGGTAAAAAAATACTTTTTTTTTATAGCTCACTATATTTATTTAGGTATTATTACTACTACGAACCGGAGCGTACCGAAGCTGTATACCCCTATACGTACCAAATTCTTATCCTGTCGCGTACCGCGAACCCGTACCCGTACCGCGTACCGGAACGAACCGCGAATTATGTGACTATGCTCAGAGCATCACAGTCACCGACCTTAAAAGCCGAGAATGGAGGTACACTTATACCTACGCGAAACTCAGTAGATTACATACTGGCTGGTCTTACTTTGTACAAGAGAAGAAACTCGTCACCAACGATTCTGTTGTTTTCATTAAAAACTCTGCTGGAAAGATATTTGTCGGAATTCGCAGGAATACCAAGTTCACTACAGATGAAGCGGGTAAAGAAGGAGGGAATTTAACTGAGATTGCAGTTTTAGATGCAGCGGAACTGGCGGAGAAGAATACAGTGTTTGAAGTTGTGTATTATCCGACAGCTAGTGGTTGGCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAGCTTCCTTTGAAGAAAGATGAATCTTCAAATTCAAAGATGACATATTCTCAGCCTAAAGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACTTATGCTATCTTATTTTGGTCTTGTTATATTTCAAGTGCATATTCTTATCAAGTTTATAGTTTTTACCAAATTTGAATCCATAATAAGATTATTTGATTGATAAATATGAGTGTTGAAGTTAATACATGATTCATTTAGATATCTAAAATTATGAAACTTAGTAGGCTAGCTTTGTGCCCTGTAGGATAAGTTCCAAATGTAATTTGAAAATTAAGAGACAGAAAATTGTTACAGAATCTCCATCTCTAACATTAATAATTCCCTTGTTCTTGCACTTGATAGATATCTCATGACACTTAATGTATACTTTATGTAATCAGATTACATTTTAGAATCGATTGTTTGTATCACACCCGTTTTTATATGTAACTCCCTACTTGACTTGCTGCAATAGTGATATTCACTAAAAAATAGTTATGAAGTTTAAGAACCTTCTAAGCATTTCTCATTGTAAATACTCCTAATGCTAAGTAGAATCTTGATTTTGGCACATGTTTTTTTGAAGAAGCTAAATTAGCCCACCTAAATTGGCACCGGAGAGAATCGAACATGAGATCTCAAGGAGGAGCACACTCCCAGATCCCAAGCCAATACCACTAGGCAACCCAAGTGGGTTTGATTTTGGCACATGTTATTAATCGATAAGAAACTTTTGATGTCTAAATACATATATACTATATATAATTTATCAAATATTTCAAATGATTGATATTGATTCTTACAATTTTTTATCTCTTTATCATAGGTTAACTGGGATGGACCTAATATCCCACAGAATCCAAACCATCCAAACCATATCCCACAGAATCCAAACCTAGTCAATACTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCATCAATTTCCCCCCAACAAAAAATCAACTTCCCCCCAACAAAAAGTCCTAGACTTTCTCAGAGTTCTTCCCCCCAACAAATTCCACATTCTCTAATGCCAATTACACTGCCTAAATTACAGCCTCCACCGTCAACTTCCTCCTTCATTCCTATGCAGGGAGTCAGGCATGAGCATGACCATTTATCTGTATCAGATTCATCAAAAGTTTTGAATGATAAGCATGACGGATTCACTGGGAATAATTCAATGGCAGGGTTGAGTGGTACTTTGCCTGACATAGAGCTAAACCTTGGCAGTCCTAGAGGTGCTGACTCGTTGCCTCATAACCATAATAGGTCCAATGAGCCAAGCACCAAGTGA
Protein sequence

>MS.gene48069.t1

MLRIAKKCTANEFTRQLAHFNGFGFCVFRHSAVTPCIPKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNAATITILDSYRPSIPCIVSAVDLFVDPHTEEVFAKLLLTPVTDQEYYMYIEYYMYISITVTDLKSREWRYTYTYAKLSRLHTGWSYFVQEKKLVTNDSVVFIKNSAGKIFVGIRRNTKFTTDEAGKEGGNLTEIAVLDAAELAEKNTVFEVVYYPTASGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKDESSNSKMTYSQPKGTISFVFNDSSNVPNWRMLEVNWDGPNIPQNPNHPNHIPQNPNLVNTWQPPPSTSSFIPMQGVRHEHDHLSVSDSSKVLNDKHDGFTGNNSMAGLSGTLPDIELNLGSPRGADSLPHNHNRSNEPSTK